Biotechnology advances最新文献

{"title":"Hydroxybenzoic acids: Microbial metabolism, pathway engineering and products","authors":"Ingrida Kutraite ,&nbsp;Ernesta Augustiniene ,&nbsp;Naglis Malys","doi":"10.1016/j.biotechadv.2025.108571","DOIUrl":"10.1016/j.biotechadv.2025.108571","url":null,"abstract":"<div><div>Hydroxybenzoic acids (HBAs) are plant secondary metabolites exhibiting antioxidant, antiviral, anticancer and antibacterial activities. A high and constantly increasing demand for these compounds underlines the need for novel and efficient production methods, as commonly applied plant extraction and chemical synthesis approaches are susceptible to low yields and are environmentally hazardous. Switching to biotechnology and replacing petroleum-based chemicals has potential to improve eco-efficiency in sustainable bioeconomy. With the increased focus on the production of materials using renewable resources and bio-based feedstocks, microbial fermentation and engineering drives the development and optimization of sustainable bioproduction. This systematic review summarizes current knowledge of microbial HBAs metabolism and biosynthesis. Here, the existing challenges are highlighted and the potential strategies for improved microbial production of HBAs are identified. Key aspects of HBAs metabolism and complexity of the factors related to bacterial strain selection, titer, and bioprocess strategy are examined. The opportunities of HBAs bioproduction using engineered microbial cell factories are discussed in detail and insights for synthesis improvement are presented.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108571"},"PeriodicalIF":12.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in gene editing-led route for hybrid breeding in crops","authors":"Muhammad Jawad Akbar Awan ,&nbsp;Muhammad Awais Farooq ,&nbsp;Muhammad Ismail Buzdar ,&nbsp;Asma Zia ,&nbsp;Aiman Ehsan ,&nbsp;Muhammad Abu Bakar Waqas ,&nbsp;Goetz Hensel ,&nbsp;Imran Amin ,&nbsp;Shahid Mansoor","doi":"10.1016/j.biotechadv.2025.108569","DOIUrl":"10.1016/j.biotechadv.2025.108569","url":null,"abstract":"<div><div>With the global demand for sustainable agriculture on the rise, RNA-guided nuclease technology offers transformative applications in crop breeding. Traditional hybrid breeding methods, like three-line and two-line systems, are often labor-intensive, transgenic, and economically burdensome. While chemical mutagens facilitate these systems, they not only generate weak alleles but also produce strong alleles that induce permanent sterility through random mutagenesis. In contrast, RNA-guided nuclease system, such as clustered regularly interspaced short palindromic repeats (CRISPR)- associated protein (Cas) system, facilitates more efficient hybrid production by inducing male sterility through targeted genome modifications in male sterility genes, such as <em>MS8</em>, <em>MS10</em>, <em>MS26</em>, and <em>MS45</em> which allows precise manipulation of pollen development or pollen abortion in various crops. Moreover, this approach allows haploid induction for the rapid generation of recombinant and homozygous lines from hybrid parents by editing essential genes, like <em>CENH3</em>, <em>MTL</em>/<em>NLD</em>/<em>PLA</em>, and <em>DMP</em>, resulting in high-yield, transgene-free hybrids. Additionally, this system supports synthetic apomixis induction by employing the <em>MiMe</em> (<em>Mitosis instead of Meiosis</em>) strategy, coupled with parthenogenesis in hybrid plants, to create heterozygous lines and retain hybrid vigor in subsequent generations. RNA-guided nuclease-induced synthetic apomixis also enables genome stacking for autopolyploid progressive heterosis <em>via</em> clonal gamete production for trait maintenance to enhance crop adaptability without compromising yield. Additionally, CRISPR-Cas-mediated <em>de novo</em> domestication of wild relatives, along with recent advances to circumvent tissue culture- recalcitrance and -dependency through heterologous expression of morphogenic regulators, holds great promise for incorporating diversity-enriched germplasm into the breeding programs. These approaches aim to generate elite hybrids adapted to dynamic environments and address the anticipated challenges of food insecurity.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108569"},"PeriodicalIF":12.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in antimicrobial peptides: From mechanistic insights to chemical modifications","authors":"Ren Yang ,&nbsp;Xiaohan Ma ,&nbsp;Feng Peng ,&nbsp;Jin Wen ,&nbsp;Latifa W. Allahou ,&nbsp;Gareth R. Williams ,&nbsp;Jonathan C. Knowles ,&nbsp;Alessandro Poma","doi":"10.1016/j.biotechadv.2025.108570","DOIUrl":"10.1016/j.biotechadv.2025.108570","url":null,"abstract":"<div><div>This review provides a comprehensive analysis of antimicrobial peptides (AMPs), exploring their diverse sources, secondary structures, and unique characteristics. The review explores into the mechanisms underlying the antibacterial, immunomodulatory effects, antiviral, antiparasitic and antitumour of AMPs. Furthermore, it discusses the three principal synthesis pathways for AMPs and assesses their current clinical applications and preclinical research status. The paper also addresses the limitations of AMPs, including issues related to stability, resistance, and toxicity, while offering insights into strategies for their enhancement. Recent advancements in AMP research, such as chemical modifications (including amino acid sequence optimisation, terminal and side-chain modifications, PEGylation, conjugation with small molecules, conjugation with photosensitisers, metal ligands, polymerisation, cyclisation and specifically targeted antimicrobial peptides) are highlighted. The goal is to provide a foundation for the future design and optimisation of AMPs.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108570"},"PeriodicalIF":12.1,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systems biology of Haemonchus contortus – Advancing biotechnology for parasitic nematode control","authors":"Yuanting Zheng ,&nbsp;Neil D. Young ,&nbsp;Tao Wang ,&nbsp;Bill C.H. Chang ,&nbsp;Jiangning Song ,&nbsp;Robin B. Gasser","doi":"10.1016/j.biotechadv.2025.108567","DOIUrl":"10.1016/j.biotechadv.2025.108567","url":null,"abstract":"<div><div>Parasitic nematodes represent a substantial global burden, impacting animal health, agriculture and economies worldwide. Of these worms, <em>Haemonchus contortus</em> – a blood-feeding nematode of ruminants – is a major pathogen and a model for molecular and applied parasitology research. This review synthesises some key advances in understanding the molecular biology, genetic diversity and host-parasite interactions of <em>H. contortus</em>, highlighting its value for comparative studies with the free-living nematode <em>Caenorhabditis elegans</em>. Key themes include recent developments in genomic, transcriptomic and proteomic technologies and resources, which are illuminating critical molecular pathways, including the ubiquitination pathway, protease/protease inhibitor systems and the secretome of <em>H. contortus</em>. Some of these insights are providing a foundation for identifying essential genes and exploring their potential as targets for novel anthelmintics or vaccines, particularly in the face of widespread anthelmintic resistance. Advanced bioinformatic tools, such as machine learning (ML) algorithms and artificial intelligence (AI)-driven protein structure prediction, are enhancing annotation capabilities, facilitating and accelerating analyses of gene functions, and biological pathways and processes. This review also discusses the integration of these tools with cutting-edge single-cell sequencing and spatial transcriptomics to dissect host-parasite interactions at the cellular level. The discussion emphasises the importance of curated databases, improved culture systems and functional genomics platforms to translate molecular discoveries into practical outcomes, such as novel interventions. New research findings and resources not only advance research on <em>H. contortus</em> and related nematodes but may also pave the way for innovative solutions to the global challenges with anthelmintic resistance.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108567"},"PeriodicalIF":12.1,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymatic polymerization: Recent advances toward sustainable polymer synthesis","authors":"Bo Xia ,&nbsp;Honghao Chen ,&nbsp;Juntao Wang ,&nbsp;Yan Liu ,&nbsp;Qi Wu ,&nbsp;Xiaocheng Pan","doi":"10.1016/j.biotechadv.2025.108566","DOIUrl":"10.1016/j.biotechadv.2025.108566","url":null,"abstract":"<div><div>Enzymatic polymerization has emerged as a sustainable strategy for synthesizing biodegradable, biocompatible polymers, addressing critical environmental challenges posed by conventional petroleum-based materials. This review comprehensively explores advancements from the past five years, spotlighting six pivotal enzymes lipase, horseradish peroxidase, laccase, glucose oxidase, glucosyltransferase, and phosphorylase-alongside synergistic multi-enzymatic systems that enable complex polymerization cascades. Diverging from prior reviews focused on individual enzymes or specific polymer classes (e.g., polyesters, polyamides), our work provides a systematic classification of enzymatic polymerization mechanisms, emphasizing substrate specificity, reaction efficiency, and product diversity. Integrating advances in enzyme engineering, cascade catalysis, and green chemistry, this analysis outlines strategies to customize polymer architectures, identifies challenges in scaling enzymatic processes, and underscores opportunities for industrial applications. It advocates interdisciplinary innovation to advance sustainable polymer synthesis aligned with circular economy principles, emphasizing enzymatic methods' transformative potential for eco-friendly manufacturing paradigms.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108566"},"PeriodicalIF":12.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of nanomaterials on microbial metabolism and their applications in fermentative hydrogen production: A review","authors":"Yanjing Li,&nbsp;Minmin Wang,&nbsp;Qiushi Jiang,&nbsp;Sihu Zhang,&nbsp;Xueying Yang,&nbsp;Wen Cao,&nbsp;Wenwen Wei,&nbsp;Liejin Guo","doi":"10.1016/j.biotechadv.2025.108563","DOIUrl":"10.1016/j.biotechadv.2025.108563","url":null,"abstract":"<div><div>Recent developments in nanomaterial-microbe hybrid systems have combined the unique physicochemical properties of nanomaterials with the biocatalytic capabilities of microorganisms. These hybrid systems have seen extensive use in energy production, particularly in enhancing hydrogen generation. Researchers have incorporated nanomaterials into microbial cultures, achieving significant improvements in the hydrogen production efficiency of microbes across various environments and bacterial strains. However, challenges such as the biological toxicity of nanomaterials pose obstacles to their broader application in microbial energy production. This review examines the effects of nanomaterials on microorganisms, focusing on both their positive and negative effects on microbial growth and metabolism. It also summarizes the applications of nanomaterials in microbial fermentation for hydrogen production. Additionally, it highlights the importance of understanding and balancing these effects when introducing nanomaterials, offering guidance for developing more efficient nanomaterial-microbial hybrid hydrogen production systems.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108563"},"PeriodicalIF":12.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-replicating nanomaterials as a new generation of smart nanostructures","authors":"Akbar Hasanzadeh ,&nbsp;Sara Saeedi ,&nbsp;Lida Dastanpour ,&nbsp;Zahra S. Biabanaki ,&nbsp;Leili Asadi ,&nbsp;Hamid Noori ,&nbsp;Michael R. Hamblin ,&nbsp;Yong Liu ,&nbsp;Mahdi Karimi","doi":"10.1016/j.biotechadv.2025.108565","DOIUrl":"10.1016/j.biotechadv.2025.108565","url":null,"abstract":"<div><div>Self-replication is the process by which a system or entity autonomously reproduces or generates copies of itself, transmitting hereditary information through its molecular structure. Self-replication can be attractive for various researchers, ranging from biologists focused on uncovering the origin of life, to synthetic chemists and nanotechnologists studying synthetic machines and nanorobots. The capability of a single structure to act as a template to produce multiple copies of itself could allow the bottom-up engineering of progressively complex reaction networks and nanoarchitectures from simple building blocks. Herein, we review nucleic acid-based and amino acid-based self-replicating systems and completely synthetic artificial systems and specially focused on specific aspects of self-replicating nanomaterials. We describe their mechanisms of action and provide a full discussion of the principal requirements for achieving nanostructures capable of self-replication.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108565"},"PeriodicalIF":12.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143661856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism and improvement of yeast tolerance to biomass-derived inhibitors: A review","authors":"Jinling Yu ,&nbsp;Cuili Li ,&nbsp;Yajie Cheng ,&nbsp;Shaobo Guo ,&nbsp;Hongzhao Lu ,&nbsp;Xiuchao Xie ,&nbsp;Hao Ji ,&nbsp;Yanming Qiao","doi":"10.1016/j.biotechadv.2025.108562","DOIUrl":"10.1016/j.biotechadv.2025.108562","url":null,"abstract":"<div><div>Lignocellulosic biomass is regarded as a potentially valuable second-generation biorefinery feedstock. Yeast has the ability to metabolize this substrate and convert it into fuel ethanol and an array of other chemical products. Nevertheless, during the pretreatment of lignocellulosic biomass, inhibitors (furanaldehydes, carboxylic acids, phenolic compounds, etc.) are generated, which impede the growth and metabolic activities of yeast cells. Consequently, developing yeast strains with enhanced tolerance to these inhibitors is a crucial technological objective, as it can significantly enhance the efficiency of lignocellulosic biorefineries. This review provides a concise overview of the process of inhibitor generation and the detrimental effects of these inhibitors on yeast. It also summarizes the current state of research on the mechanisms of yeast tolerance to these inhibitors, focusing specifically on recent advances in enhancing yeast tolerance to these inhibitors by rational and non-rational strategies. Finally, it discusses the current challenges and future research directions.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108562"},"PeriodicalIF":12.1,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143637183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Re-imagining human cell culture media: Challenges, innovations, and future directions","authors":"Akila Wijerathna-Yapa ,&nbsp;Kathy Sharon Isaac ,&nbsp;Michelle Combe ,&nbsp;Samuel Hume ,&nbsp;Stanislav Sokolenko","doi":"10.1016/j.biotechadv.2025.108564","DOIUrl":"10.1016/j.biotechadv.2025.108564","url":null,"abstract":"<div><div>The development of optimized culture media is pivotal to advancements in human cell culture, underpinning progress in regenerative medicine, cell therapies, and personalized medicine. While foundational formulations like Eagle's Minimum Essential Medium (MEM) and Dulbecco's Modified Eagle Medium (DMEM) have historically enabled significant biological research, these media were primarily designed for non-human cells and do not adequately address the unique metabolic and functional requirements of human cells. This review examines the evolution of cell culture media, identifying persistent challenges in reproducibility, scalability, and ethical concerns, particularly regarding the reliance on animal-derived components such as fetal bovine serum (FBS).</div><div>We highlight innovations in serum-free and chemically defined media that offer promising alternatives by enhancing consistency, aligning with Good Manufacturing Practices, and addressing ethical concerns. Emerging approaches, including omics-based profiling, high-throughput screening, and artificial intelligence (AI)-driven media design, are reshaping media optimization by enabling precise tailoring to the needs of specific human cell types and patient-derived cells. Furthermore, we discuss economic and regulatory challenges, emphasizing the need for cost-effective and scalable solutions to facilitate clinical translation.</div><div>Looking forward, integrating advanced biotechnological tools such as 3D bioprinting, organ-on-a-chip systems, and personalized media formulations presents a transformative opportunity for human cell culture. These innovations, aligned with ethical and clinical standards, can drive the development of human-specific media systems that ensure reproducibility, scalability, and enhanced therapeutic potential, thereby advancing both research and clinical applications.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108564"},"PeriodicalIF":12.1,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in the application of CRISPR/Cas-based gene editing technology in Filamentous Fungi","authors":"Xu-Hong Li,&nbsp;Hui-Zhi Lu,&nbsp;Ji-Bao Yao,&nbsp;Chi Zhang,&nbsp;Tian-Qiong Shi,&nbsp;He Huang","doi":"10.1016/j.biotechadv.2025.108561","DOIUrl":"10.1016/j.biotechadv.2025.108561","url":null,"abstract":"<div><div>Filamentous fungi are essential industrial microorganisms that can serve as sources of enzymes, organic acids, terpenoids, and other bioactive compounds with significant applications in food, medicine, and agriculture. However, the underdevelopment of gene editing tools limits the full exploitation of filamentous fungi, which still present numerous untapped potential applications. In recent years, the CRISPR/Cas (clustered regularly interspaced short palindromic repeats) system, a versatile genome-editing tool, has advanced significantly and been widely applied in filamentous fungi, showcasing considerable research potential. This review examines the development and mechanisms of genome-editing tools in filamentous fungi, and contrasts the CRISPR/Cas9 and CRISPR/Cpf1 systems. The transformation and delivery strategies of the CRISPR/Cas system in filamentous fungi are also examined. Additionally, recent applications of CRISPR/Cas systems in filamentous fungi are summarized, such as gene disruption, base editing, and gene regulation. Strategies to enhance editing efficiency and reduce off-target effects are also highlighted, with the aim of providing insights for the future construction and optimization of CRISPR/Cas systems in filamentous fungi.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108561"},"PeriodicalIF":12.1,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143620599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}