Biotechnology advances最新文献

{"title":"Enzyme miniaturization: Revolutionizing future biocatalysts.","authors":"Ning Ding, Yaoyukun Jiang, Sangsin Lee, Zihao Cheng, Xinchun Ran, Yujing Ding, Robbie Ge, Yifei Zhang, Zhongyue J Yang","doi":"10.1016/j.biotechadv.2025.108598","DOIUrl":"https://doi.org/10.1016/j.biotechadv.2025.108598","url":null,"abstract":"<p><p>Enzyme miniaturization offers a transformative approach to overcome limitations posed by the large size of conventional enzymes in industrial, therapeutic, and diagnostic applications. However, the evolutionary optimization of enzymes for activity and stability has not inherently favored compact structures, creating challenges for modern applications requiring smaller and more efficient catalysts. In this review, we surveyed the advantages of miniature enzymes, including enhanced expressivity, folding efficiency, thermostability, and resistance to proteolysis. We described the applications of miniature enzymes as biosensors, therapeutic agents, and industrial catalysts. We highlighted strategies such as genome mining, rational design, random deletion, and de novo design for achieving enzyme miniaturization, integrating both computational and experimental techniques. By investigating these approaches, we aim to provide a framework for advancing enzyme engineering, emphasizing the unique potential of smaller enzymes to revolutionize biocatalysis, gene therapy, and biosensing technologies.</p>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":" ","pages":"108598"},"PeriodicalIF":12.1,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rich analytic toolbox for the exploration, characterization, screening, and application studies of ω-transaminases","authors":"Guan Zhou ,&nbsp;Zewei Zhou ,&nbsp;Dandan Feng ,&nbsp;Wenrui Fan ,&nbsp;Quan Luo ,&nbsp;Xuefeng Lu","doi":"10.1016/j.biotechadv.2025.108597","DOIUrl":"10.1016/j.biotechadv.2025.108597","url":null,"abstract":"<div><div>Omega-transaminases (ωTAs) constitute an important class of biocatalysts in the pharmaceutical, agrochemical, and fine chemical industries, because of their generally good performance in the efficient, enantiospecific, and environment-friendly synthesis of chiral amines that possess diverse chemical structures and biological activities. However, their practical applications are often hindered by unfavorable reaction equilibria, product inhibition, limited robustness, and relatively small accommodation for substrates. Many efforts, including the exploration of novel enzymes from various environments and the targeted engineering of identified enzymes, have been made to develop more specific and efficient ωTA catalysts. A simple, rapid, and accurate evaluation of enzyme activity is important. In addition to the classic chromatography-based methods, to date, at least 18 analytic methods, which are based on cell growth or colorimetry/spectrophotometry, pH, fluorescence and conductivity changes, have been developed and applied in both qualitative and quantitative analyses of ωTAs. These methods differ in terms of their principles, accuracy, throughput, simplicity, and cost-effectiveness. Here, we present a detailed examination of the advantages and drawbacks of these methods. Guidance for method selection from the perspective of practical applications is proposed to assist investigators in choosing appropriate methods according to different research purposes and existing conditions.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"82 ","pages":"Article 108597"},"PeriodicalIF":12.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myxobacteria: Versatile cell factories of novel commercial enzymes for bio-manufacturing","authors":"Zhoukun Li ,&nbsp;Lei Zhang ,&nbsp;Xianfeng Ye ,&nbsp;Yan Huang ,&nbsp;Yanling Ji ,&nbsp;Yuezhong Li ,&nbsp;Daniel Wall ,&nbsp;Zhongli Cui","doi":"10.1016/j.biotechadv.2025.108594","DOIUrl":"10.1016/j.biotechadv.2025.108594","url":null,"abstract":"<div><div>Microbial cell factories for the production of high<strong>-</strong>quality commercial-grade enzymes have accelerated the development of advanced bio-manufacturing approaches, which in turn are environmentally friendly and sustainable. Myxobacteria, a term commonly used to refer to a group within the <em>Myxococcota</em> phylum, are of great interest for their biotechnological applications due to their ability to synthesize a wide range of natural products and lytic enzymes. These traits are essential for the development of robust expression systems. However, myxobacteria have remained an underexploited resource with industrial relevance. Nevertheless, a growing number of food and industrial enzymes have been identified, highlighting myxobacteria as suitable platforms for exploring enzymes with commercial applications, including biomass conversion. Yet, the discovered lytic enzymes are just the tip of the iceberg given their large genomes and diversity across myxobacteria taxa. Despite holding much promise, challenges in genetic engineering, slow growth, and limitations in metabolic remodeling and expression strategies have limited the construction of myxobacterial cell factories. In this review, we highlight recent advances in the discovery of new myxobacterial enzymes and biomass conversion resources, focusing on their potential applications in agriculture and industry. We describe how myxobacteria and their enzymes can be identified through bioprospecting and computational approaches and summarize current biotechnological applications and synthetic biology strategies for bio-manufacturing. Finally, we discuss the promising potential of myxobacteria as industrial cell factories and address open research questions and future directions.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"82 ","pages":"Article 108594"},"PeriodicalIF":12.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Technological advancement spurs Komagataella phaffii as a next-generation platform for sustainable biomanufacturing","authors":"Le Gao ,&nbsp;Jie Yuan ,&nbsp;Kai Hong ,&nbsp;Nyuk Ling Ma ,&nbsp;Shuguang Liu ,&nbsp;Xin Wu","doi":"10.1016/j.biotechadv.2025.108593","DOIUrl":"10.1016/j.biotechadv.2025.108593","url":null,"abstract":"<div><div>Biomanufacturing stands as a cornerstone of sustainable industrial development, necessitating a shift toward non-food carbon feedstocks to alleviate agricultural resource competition and advance a circular bioeconomy. Methanol, a renewable one‑carbon substrate, has emerged as a pivotal candidate due to its abundance, cost-effectiveness, and high reduction potential, further bolstered by breakthroughs in CO₂ hydrogenation-based synthesis. Capitalizing on this momentum, the methylotrophic yeast <em>Komagataella phaffii</em> has undergone transformative technological upgrades, evolving from a conventional protein expression workhorse into an intelligent bioproduction chassis. This paradigm shift is fundamentally driven by converging innovations across CRISPR-empowered advancement in genome editing and AI-powered metabolic pathway design in <em>K. phaffii.</em> The integration of CRISPR systems with droplet microfluidics high-throughput screening has redefined strain engineering efficiency, achieving much higher editing precision than traditional homologous recombination while compressing the “design-build-test-learn” cycle. Concurrently, machine learning-enhanced genome-scale metabolic models facilitate dynamic flux balancing, enabling simultaneous improvements in product titers, carbon yields, and volumetric productivity. Finally, technological advancement promotes the application of <em>K. phaffii</em>, including directing more efficiently metabolic flux toward nutrient products, and strengthening efficient synthesis of excreted proteins. As DNA synthesis automation and robotic experimentation platforms mature, next-generation breakthroughs in genome modification, cofactor engineering, and AI-guided autonomous evolution will further cement <em>K. phaffii</em> as a next-generation platform for decarbonizing global manufacturing paradigms. This technological trajectory positions methanol-based biomanufacturing as a cornerstone of the low-carbon circular economy.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"82 ","pages":"Article 108593"},"PeriodicalIF":12.1,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteria-microalgae interactions from an evolutionary perspective and their biotechnological significance","authors":"Ahmad Abd-El-Aziz ,&nbsp;Sherif M. Elnagdy ,&nbsp;Jichang Han ,&nbsp;Rok Mihelič ,&nbsp;Xulei Wang ,&nbsp;Spiros N. Agathos ,&nbsp;Jian Li","doi":"10.1016/j.biotechadv.2025.108591","DOIUrl":"10.1016/j.biotechadv.2025.108591","url":null,"abstract":"<div><div>Interactions between bacteria and microalgae have been studied in natural environments and in industrial consortia. As results of co-evolution for millions of years in nature, they have developed complex symbiotic relationships, including mutualism, commensalism and parasitism, the nature of which is decided by mechanisms of the interaction. There are two main types of molecular interactions between microalgae and bacteria: exchange of nutrients and release of signalling molecules. Nutrient exchange includes transport of organic carbon from microalgae to bacteria and nutrient nitrogen released from nitrogen-fixing bacteria to microalgae, as well as reciprocal supply of micronutrients such as B vitamins and iron. Signalling molecules such as phytohormones secreted by microalgae and quorum sensing molecules secreted by bacteria have been shown to positively affect growth and metabolism of the symbiotic partner. However, there are still a number of potential microalgae-bacteria interactions that have not been well explored, including cyclic peptides, other quorum signalling molecules, and extracellular vesicles involved in exchange of genetic materials. A more thorough understanding of these interactions may not only result in a deeper understanding of the relationships between these symbiotic organisms but also have potential biotechnological applications. Upon new mechanisms of interaction being identified and characterized, novel bioprocesses of synthetic ecology might be developed especially for wastewater treatment and production of biofertilizers and biofuels.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"82 ","pages":"Article 108591"},"PeriodicalIF":12.1,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracking inflammation status for improving patient prognosis: A review of current methods, unmet clinical needs and opportunities","authors":"Vidya Raju ,&nbsp;Revanth Reddy ,&nbsp;Arzhang Cyrus Javan ,&nbsp;Behnam Hajihossainlou ,&nbsp;Ralph Weissleder ,&nbsp;Anthony Guiseppi-Elie ,&nbsp;Katsuo Kurabayashi ,&nbsp;Simon A. Jones ,&nbsp;Rose T. Faghih","doi":"10.1016/j.biotechadv.2025.108592","DOIUrl":"10.1016/j.biotechadv.2025.108592","url":null,"abstract":"<div><div>Inflammation is the body's response to infection, trauma or injury and is activated in a coordinated fashion to ensure the restoration of tissue homeostasis and healthy physiology. This process requires communication between stromal cells resident to the tissue compartment and infiltrating immune cells which is dysregulated in disease. Clinical innovations in patient diagnosis and stratification include measures of inflammatory activation that support the assessment of patient prognosis and response to therapy. We propose that (i) the recent advances in fast, dynamic monitoring of inflammatory markers (e.g., cytokines) and (ii) data-dependent theoretical and computational modeling of inflammatory marker dynamics will enable the quantification of the inflammatory response, identification of optimal, disease-specific biomarkers and the design of personalized interventions to improve patient outcomes - multidisciplinary efforts in which biomedical engineers may potentially contribute. To illustrate these ideas, we describe the actions of cytokines, acute phase proteins and hormones in the inflammatory response and discuss their role in local wounds, COVID-19, cancer, autoimmune diseases, neurodegenerative diseases and aging, with a central focus on cardiac surgery. We also discuss the challenges and opportunities involved in tracking and modulating inflammation in clinical settings.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"82 ","pages":"Article 108592"},"PeriodicalIF":12.1,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guidelines toward ecologically-informed bioprospecting for microbial plastic degradation","authors":"Feng M. Cai ,&nbsp;Siqi Jiang ,&nbsp;Paul Daly ,&nbsp;Mounes Bakhshi ,&nbsp;Kai Cartwright ,&nbsp;Irina S. Druzhinina","doi":"10.1016/j.biotechadv.2025.108590","DOIUrl":"10.1016/j.biotechadv.2025.108590","url":null,"abstract":"<div><div>Biological degradation of plastics by microbial enzymes offers a sustainable alternative to traditional waste management methods that often pollute the environment. This review explores ecologically-informed bioprospecting for microorganisms possessing enzymes suitable for biological plastic waste treatment. Natural habitats enriched in plastic-like polymers, such as insect-derived polyesters, epicuticular microbial biofilms in the phyllosphere of plants in extreme environments, or aquatic ecosystems, are highlighted as promising reservoirs for bioprospecting. Anthropogenic habitats, including plastic-polluted soils and the plastisphere, have yielded potent enzymes such as PETases and cutinases, which are being exploited in biotechnology. However, bioprospecting in plastispheres and artificial environments frequently leads to the isolation of environmental opportunistic microorganisms, such as <em>Pseudomonas aeruginosa</em>, <em>Aspergillus fumigatus</em>, <em>Parengyodontium album</em>, or species of <em>Fusarium</em>, which are capable of becoming human and/or plant pathogens. These cases necessitate stringent biosecurity measures, including accurate molecular identification, ecological assessment, and containment protocols. Beyond advancing bioprospecting approaches toward a broader scope of relevant habitats, this review underscores the educational value of such screenings, specifically, in understudied natural habitats, emphasizing its potential to uncover novel enzymes and microorganisms and engage the next generation of researchers in interdisciplinary study integrating environmental microbiology, molecular biology, enzymology, polymer chemistry, and bioinformatics. Finally, we offer guidelines for microbial bioprospecting in various laboratory settings, ranging from standard environmental microbiology facilities to high-biosecurity facilities, thereby maximizing the diversity of scientists who may contribute to addressing urgent environmental challenges associated with plastic waste.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"82 ","pages":"Article 108590"},"PeriodicalIF":12.1,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on the expanding biotechnological frontier of Pedobacter","authors":"Gonçalo Figueiredo ,&nbsp;Hugo Osório ,&nbsp;Marta V. Mendes ,&nbsp;Sónia Mendo","doi":"10.1016/j.biotechadv.2025.108588","DOIUrl":"10.1016/j.biotechadv.2025.108588","url":null,"abstract":"<div><div>The genus <em>Pedobacter</em> consists of Gram-negative bacteria with a broad geographic distribution, isolated from diverse habitats, including water, soil, plants, wood, rocks and animals. However, characterization efforts have been limited to a small number of species. Likewise, in the context of natural products (NP), only a small fraction of <em>Pedobacter</em> -derived NPs have been characterized so far. In contrast, in silico analysis of the increasing number of available genomes in the databases, suggests a wealth of yet to be discovered compounds. Notable biotechnological applications described so far include the production of heparinases and chondroitinases for therapeutic purposes, phytases and galactosidases as aquaculture feed supplements, alginate lyases for biofuel production, and secondary metabolites such as pedopeptins and isopedopeptins with antimicrobial properties. Further research integrating synthetic biology approaches, holds great promise for unlocking the hidden potential of members of this genus, thus expanding its industrial applications.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"82 ","pages":"Article 108588"},"PeriodicalIF":12.1,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143881542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selecting optimal algal strains for robust photosynthetic upgrading of biogas under temperate oceanic climates","authors":"Muhammad Nabeel Haider ,&nbsp;Linda O'Higgins ,&nbsp;Richard O'Shea ,&nbsp;Lorraine Archer ,&nbsp;David M. Wall ,&nbsp;Nikita Verma ,&nbsp;María del Rosario Rodero ,&nbsp;Muhammad Aamer Mehmood ,&nbsp;Jerry D. Murphy ,&nbsp;Archishman Bose","doi":"10.1016/j.biotechadv.2025.108581","DOIUrl":"10.1016/j.biotechadv.2025.108581","url":null,"abstract":"<div><div>Biogas generated from anaerobic digestion can be upgraded to biomethane by photosynthetic biogas upgrading, using CO₂ as a bioresource for algal (cyanobacteria and microalgae) cultivation. This allows the upgrading technology to offer economic and environmental benefits to conventional physiochemical upgrading techniques (which can be energy-intensive and costly) by co-generating biomethane with high-value biomass. However, a critical challenge in implementing this technology in temperate oceanic climatic conditions (as found in Japan, and the northwest coasts of Europe and of North America, with average temperatures ranging between 5 and 20 °C) is the selection of algal strains that must be capable of sustained growth under lower ambient temperatures. Accordingly, this paper investigated the selection of algae that met seven key criteria: optimal growth at high pH (9–11); at alkalinity of 1.5–2.5 g inorganic carbon per litre; operation at low temperature (5–20 °C); tolerance to high CO₂ concentrations (above 20 %); capability for mixotrophic cultivation; ability to accumulate high-value metabolites such as photosynthetic pigments and bioactive fatty acids; and ease of harvesting. Of the twenty-six algal species assessed and ranked using a Pugh Matrix, <em>Anabaena</em> sp. and <em>Phormidium</em> sp. were assessed as the most favourable species, followed by <em>Oscillatoria</em> sp., <em>Spirulina subsalsa</em>, and <em>Leptolyngbya</em> sp. Adaptive laboratory evolution together with manipulation of abiotic factors could be effectively utilised to increase the efficiency and economic feasibility of the use of the selected strain in a photosynthetic biogas upgrading system, through improvement of growth and yield of high-value compounds.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"82 ","pages":"Article 108581"},"PeriodicalIF":12.1,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143886206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomics and plant-microbe symbioses: Microbial community dynamics, functional roles in carbon sequestration, nitrogen transformation, sulfur and phosphorus mobilization for sustainable soil health","authors":"Atif Khurshid Wani ,&nbsp;Fayzan Qadir ,&nbsp;Noureddine Elboughdiri ,&nbsp;Farida Rahayu ,&nbsp;Saefudin ,&nbsp;Dibyo Pranowo ,&nbsp;Chaireni Martasari ,&nbsp;Mia Kosmiatin ,&nbsp;Cece Suhara ,&nbsp;Tri Sudaryono ,&nbsp;Yusmani Prayogo ,&nbsp;Krishna Kumar Yadav ,&nbsp;Khursheed Muzammil ,&nbsp;Lienda Bashier Eltayeb ,&nbsp;Maha Awjan Alreshidi ,&nbsp;Reena Singh","doi":"10.1016/j.biotechadv.2025.108580","DOIUrl":"10.1016/j.biotechadv.2025.108580","url":null,"abstract":"<div><div>Biogeochemical cycles are fundamental processes that regulate the flow of essential elements such as carbon, nitrogen, and phosphorus, sustaining ecosystem productivity and global biogeochemical equilibrium. These cycles are intricately influenced by plant-microbe symbioses, which facilitate nutrient acquisition, organic matter decomposition, and the transformation of soil nutrients. Through mutualistic interactions, plants and microbes co-regulate nutrient availability and promote ecosystem resilience, especially under environmental stress. Metagenomics has emerged as a transformative tool for deciphering the complex microbial communities and functional genes driving these cycles. By enabling the high-throughput sequencing and annotation of microbial genomes, metagenomics provides unparalleled insights into the taxonomic diversity, metabolic potential, and functional pathways underlying microbial contributions to biogeochemical processes. Unlike previous reviews, this work integrates recent advancements in metagenomics with complementary omics approaches to provide a comprehensive perspective on how plant-microbe interactions modulate biogeochemical cycles at molecular, genetic, and ecosystem levels. By highlighting novel microbial processes and potential biotechnological applications, this review aims to guide future research in leveraging plant-microbe symbioses for sustainable agriculture, ecosystem restoration, and climate change mitigation.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"82 ","pages":"Article 108580"},"PeriodicalIF":12.1,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}