微流体技术在生物制造过程中的应用。

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances Pub Date : 2025-09-16 DOI:10.1016/j.biotechadv.2025.108715

Federico Moreno-Sibaja, Da Zou, Sha Liu, Chun-Xia Zhao, Lukas Gerstweiler

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引用次数: 0

摘要

由于对高价值产品、化学品、生物燃料和食品的需求不断增加，生物制造工艺正在扩大其能力。生物工艺开发的最新趋势侧重于在早期阶段缩小系统的规模，以实现快速和具有成本效益的实验。微流体系统通过实现动态和高通量实验，增强过程控制，减少操作体积和时间，是生物制造过程开发的强大替代方案。微流体装置应用于整个生物处理工作流程，从上游任务（如菌株发育和细胞生长）到下游过程（包括细胞裂解和纯化）。传感器和自动化的集成对于获得高质量数据至关重要，这是过程优化和扩大的关键。本文综述了微流体的基本原理，强调了该技术在生物制造工艺开发中的主要特点。然后讨论了微流控系统在上下游工艺优化和规模化中的应用。最后，提出了挑战和未来的展望，特别是制造技术，自动化和人工智能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Microfluidics in biomanufacturing process development

Biomanufacturing processes are expanding their capabilities due to an increasing demand for high-value products, chemicals, biofuels, and food. Recent trends in bioprocess development focus on scale down systems during early-stage to enable rapid and cost-effective experimentation. Microfluidic systems are powerful alternatives for biomanufacturing process development by enabling dynamic and high-throughput experiments, enhanced process control, and reduced operational volumes and times. Microfluidic setups are applied across the entire bioprocessing workflow, from upstream tasks such as strain development and cell growth to downstream processes including cell lysis and purification. The integration of sensors and automation are critical for obtaining high-quality data, which is key for process optimization and scale-up. This review summarizes the fundamentals of microfluidics, highlighting key features of this technology for biomanufacturing process development. The application of microfluidic systems for upstream and downstream processing optimization and scale-up are then discussed. Finally, challenges and future perspectives are presented, specifically fabrication technologies, automation, and artificial intelligence.

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来源期刊

Biotechnology advances 工程技术-生物工程与应用微生物

CiteScore

25.50

自引率

2.50%

发文量

167

审稿时长

37 days

期刊介绍： Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.