Biotechnology advances最新文献_第3页

Droplet-based single-cell sequencing: Strategies and applications 基于液滴的单细胞测序：策略与应用

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-11 DOI: 10.1016/j.biotechadv.2024.108454

{"title":"Droplet-based single-cell sequencing: Strategies and applications","authors":"","doi":"10.1016/j.biotechadv.2024.108454","DOIUrl":"10.1016/j.biotechadv.2024.108454","url":null,"abstract":"<div><p>Notable advancements in single-cell omics technologies have not only addressed longstanding challenges but also enabled unprecedented studies of cellular heterogeneity with unprecedented resolution and scale. These strides have led to groundbreaking insights into complex biological systems, paving the way for a more profound comprehension of human biology and diseases. The droplet microfluidic technology has become a crucial component in many single-cell sequencing workflows in terms of throughput, cost-effectiveness, and automation. Utilizing a microfluidic chip to encapsulate and profile individual cells within droplets has significantly improved single-cell research. Therefore, this review aims to comprehensively elaborate the droplet microfluidics-assisted omics methods from a single-cell perspective. The strategies for using droplet microfluidics in the realms of genomics, epigenomics, transcriptomics, and proteomics analyses are first introduced. On this basis, the focus then turns to the latest applications of this technology in different sequencing patterns, including mono- and multi-omics. Finally, the challenges and further perspectives of droplet-based single-cell sequencing in both foundational research and commercial applications are discussed.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":null,"pages":null},"PeriodicalIF":12.1,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142242996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in engineering synthetic biomolecular condensates 合成生物分子凝聚态工程的最新进展

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-11 DOI: 10.1016/j.biotechadv.2024.108452

{"title":"Recent advances in engineering synthetic biomolecular condensates","authors":"","doi":"10.1016/j.biotechadv.2024.108452","DOIUrl":"10.1016/j.biotechadv.2024.108452","url":null,"abstract":"<div><p>Biomolecular condensates are intriguing entities found within living cells. These structures possess the ability to selectively concentrate specific components through phase separation, thereby playing a crucial role in the spatiotemporal regulation of a wide range of cellular processes and metabolic activities. To date, extensive studies have been dedicated to unraveling the intricate connections between molecular features, physical properties, and cellular functions of condensates. This collective effort has paved the way for deliberate engineering of tailor-made condensates with specific applications. In this review, we comprehensively examine the underpinnings governing condensate formation. Next, we summarize the material states of condensates and delve into the design of synthetic intrinsically disordered proteins with tunable phase behaviors and physical properties. Subsequently, we review the diverse biological functions demonstrated by synthetic biomolecular condensates, encompassing gene regulation, cellular behaviors, modulation of biochemical reactions, and manipulation of endogenous protein activities. Lastly, we discuss future challenges and opportunities in constructing synthetic condensates with tunable physical properties and customized cellular functions, which may shed light on the development of new types of sophisticated condensate systems with distinct functions applicable to various scenarios.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":null,"pages":null},"PeriodicalIF":12.1,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142228987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of marine-derived bioactive peptide molecules for industrial applications: A reverse engineering approach 为工业应用生产海洋生物活性肽分子：逆向工程方法

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-10 DOI: 10.1016/j.biotechadv.2024.108449

引用次数: 0

Advance computational tools for multiomics data learning 用于多组学数据学习的先进计算工具

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-07 DOI: 10.1016/j.biotechadv.2024.108447

引用次数: 0

From natural to synthetic: Promoter engineering in yeast expression systems 从天然到合成：酵母表达系统中的启动子工程。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-07 DOI: 10.1016/j.biotechadv.2024.108446

{"title":"From natural to synthetic: Promoter engineering in yeast expression systems","authors":"","doi":"10.1016/j.biotechadv.2024.108446","DOIUrl":"10.1016/j.biotechadv.2024.108446","url":null,"abstract":"<div><p>Synthetic promoters are particularly relevant for application not only in yeast expression systems designed for high-level heterologous protein production but also in other applications such as metabolic engineering, cell biological research, and stage-specific gene expression control. By designing synthetic promoters, researcher can create customized expression systems tailored to specific needs, whether it is maximizing protein production or precisely controlling gene expression at different stages of a process. While recognizing the limitations of endogenous promoters, they also provide important information needed to design synthetic promoters. In this review, emphasis will be placed on some key approaches to identify endogenous, and to generate synthetic promoters in yeast expression systems. It shows the connection between endogenous and synthetic promoters, highlighting how their interplay contributes to promoter development. Furthermore, this review illustrates recent developments in biotechnological advancements and discusses how this field will evolve in order to develop custom-made promoters for diverse applications. This review offers detailed information, explores the transition from endogenous to synthetic promoters, and presents valuable perspectives on the next generation of promoter design strategies.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":null,"pages":null},"PeriodicalIF":12.1,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S073497502400140X/pdfft?md5=81914edd11ad9cc6e44d7ac302f9a97f&pid=1-s2.0-S073497502400140X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142153113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An overview on polyurethane-degrading enzymes 聚氨酯降解酶概述。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-09-04 DOI: 10.1016/j.biotechadv.2024.108439

引用次数: 0

Pathway to industrial application of heterotrophic organisms in critical metals recycling from e-waste 异养生物在电子垃圾关键金属回收中的工业应用途径。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-08-30 DOI: 10.1016/j.biotechadv.2024.108438

{"title":"Pathway to industrial application of heterotrophic organisms in critical metals recycling from e-waste","authors":"","doi":"10.1016/j.biotechadv.2024.108438","DOIUrl":"10.1016/j.biotechadv.2024.108438","url":null,"abstract":"<div><p>The transition to renewable energies and electric vehicles has triggered an unprecedented demand for metals. Sustainable development of these technologies relies on effectively managing the lifecycle of critical raw materials, including their responsible sourcing, efficient use, and recycling. Metal recycling from electronic waste (e-waste) is of paramount importance owing to ore-exceeding amounts of critical elements and high toxicity of heavy metals and organic pollutants in e-waste to the natural ecosystem and human body. Heterotrophic microbes secrete numerous metal-binding biomolecules such as organic acids, amino acids, cyanide, siderophores, peptides, and biosurfactants which can be utilized for eco-friendly and profitable metal recycling. In this review paper, we presented a critical review of heterotrophic organisms in biomining, and current barriers hampering the industrial application of organic acid bioleaching and biocyanide leaching. We also discussed how these challenges can be surmounted with simple methods (e.g., culture media optimization, separation of microbial growth and metal extraction process) and state-of-the-art biological approaches (e.g., artificial microbial community, synthetic biology, metabolic engineering, advanced fermentation strategies, and biofilm engineering). Lastly, we showcased emerging technologies (e.g., artificially synthesized peptides, siderophores, and biosurfactants) derived from heterotrophs with the potential for inexpensive, low-impact, selective and advanced metal recovery from bioleaching solutions.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":null,"pages":null},"PeriodicalIF":12.1,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0734975024001320/pdfft?md5=2a7edaf45f27d2d6647833907e78532e&pid=1-s2.0-S0734975024001320-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142104016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational strategies in Klebsiella pneumoniae vaccine design: navigating the landscape of in silico insights. 肺炎克雷伯氏菌疫苗设计中的计算策略：浏览硅学洞察的全貌。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-08-30 DOI: 10.1016/j.biotechadv.2024.108437

{"title":"Computational strategies in Klebsiella pneumoniae vaccine design: navigating the landscape of in silico insights.","authors":"","doi":"10.1016/j.biotechadv.2024.108437","DOIUrl":"10.1016/j.biotechadv.2024.108437","url":null,"abstract":"<div><p>The emergence of multidrug-resistant <em>Klebsiella pneumoniae</em> poses a grave threat to global public health, necessitating urgent strategies for vaccine development. In this context, computational tools have emerged as indispensable assets, offering unprecedented insights into klebsiellal biology and facilitating the design of effective vaccines. Here, a review of the application of computational methods in the development of <em>K. pneumoniae</em> vaccines is presented, elucidating the transformative impact of in silico approaches. Through a systematic exploration of bioinformatics, structural biology, and immunoinformatics techniques, the complex landscape of <em>K. pneumoniae</em> pathogenesis and antigenicity was unravelled. Key insights into virulence factors, antigen discovery, and immune response mechanisms are discussed, highlighting the pivotal role of computational tools in accelerating vaccine development efforts. Advancements in epitope prediction, antigen selection, and vaccine design optimisation are examined, highlighting the potential of in silico approaches to update vaccine development pipelines. Furthermore, challenges and future directions in leveraging computational tools to combat <em>K. pneumoniae</em> are discussed, emphasizing the importance of multidisciplinary collaboration and data integration. This review provides a comprehensive overview of the current state of computational contributions to <em>K. pneumoniae</em> vaccine development, offering insights into innovative strategies for addressing this urgent global health challenge.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":null,"pages":null},"PeriodicalIF":12.1,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142095178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms 转基因植物和重组微生物在生产特殊脂肪酸方面的生物技术进步

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-08-28 DOI: 10.1016/j.biotechadv.2024.108435

{"title":"Biotechnological advances in the production of unusual fatty acids in transgenic plants and recombinant microorganisms","authors":"","doi":"10.1016/j.biotechadv.2024.108435","DOIUrl":"10.1016/j.biotechadv.2024.108435","url":null,"abstract":"<div><p>Certain plants and microorganisms can produce high amounts of unusual fatty acids (UFAs) such as hydroxy, conjugated, cyclic, and very long-chain polyunsaturated fatty acids, which have distinct physicochemical properties and significant applications in the food, feed, and oleochemical industries. Since many natural sources of UFAs are not ideal for large-scale agricultural production or fermentation, it is attractive to produce them through synthetic biology. Although several UFAs have been commercially or pre-commercially produced in transgenic plants and microorganisms, their contents in transgenic hosts are generally much lower than in natural sources. Moreover, reproducing this success for a wider spectrum of UFAs has remained challenging. This review discusses recent advancements in our understanding of the biosynthesis, accumulation, and heterologous production of UFAs, and addresses the challenges and potential strategies for achieving high UFA content in engineered plants and microorganisms.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":null,"pages":null},"PeriodicalIF":12.1,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0734975024001290/pdfft?md5=d382e77cfb86a2151f88191de72ef94b&pid=1-s2.0-S0734975024001290-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142095355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in the construction strategy, functional properties, and biosensing application of self-assembled triangular unit-based DNA nanostructures 基于自组装三角形单元的 DNA 纳米结构的构建策略、功能特性和生物传感应用的最新进展。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-08-28 DOI: 10.1016/j.biotechadv.2024.108436

{"title":"Recent advances in the construction strategy, functional properties, and biosensing application of self-assembled triangular unit-based DNA nanostructures","authors":"","doi":"10.1016/j.biotechadv.2024.108436","DOIUrl":"10.1016/j.biotechadv.2024.108436","url":null,"abstract":"<div><p>Research on self-assembled deoxyribonucleic acid (DNA) nanostructures with different shapes, sizes, and functions has recently made rapid progress owing to its biocompatibility, programmability, and stability. Among these, triangular unit-based DNA nanostructures, which are typically multi-arm DNA tiles, have been widely applied because of their unique structural rigidity, spatial flexibility, and cell permeability. Triangular unit-based DNA nanostructures are folded from multiple single-stranded DNA using the principle of complementary base pairing. Its shape and size can be determined using pre-set scaffold strands, segmented base complementary regions, and sequence lengths. The resulting DNA nanostructures retain the desired sequence length to serve as binding sites for other molecules and obtain satisfactory results in molecular recognition, spatial orientation, and target acquisition. Therefore, extensive research on triangular unit-based DNA nanostructures has shown that they can be used as powerful tools in the biosensing field to improve specificity, sensitivity, and accuracy. Over the past few decades, various design strategies and assembly techniques have been established to improve the stability, complexity, functionality, and practical applications of triangular unit-based DNA nanostructures in biosensing. In this review, we introduce the structural design strategies and principles of typical triangular unit-based DNA nanostructures, including triangular, tetrahedral, star, and net-shaped DNA. We then summarize the functional properties of triangular unit-based DNA nanostructures and their applications in biosensing. Finally, we critically discuss the existing challenges and future trends.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":null,"pages":null},"PeriodicalIF":12.1,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142104017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0