Biotechnology advances最新文献

{"title":"Tracking inflammation status for improving patient prognosis: A review of current methods, unmet clinical needs and opportunities","authors":"Vidya Raju ,&nbsp;Revanth Reddy ,&nbsp;Arzhang Cyrus Javan ,&nbsp;Behnam Hajihossainlou ,&nbsp;Ralph Weissleder ,&nbsp;Anthony Guiseppi-Elie ,&nbsp;Katsuo Kurabayashi ,&nbsp;Simon A. Jones ,&nbsp;Rose T. Faghih","doi":"10.1016/j.biotechadv.2025.108592","DOIUrl":"10.1016/j.biotechadv.2025.108592","url":null,"abstract":"<div><div>Inflammation is the body's response to infection, trauma or injury and is activated in a coordinated fashion to ensure the restoration of tissue homeostasis and healthy physiology. This process requires communication between stromal cells resident to the tissue compartment and infiltrating immune cells which is dysregulated in disease. Clinical innovations in patient diagnosis and stratification include measures of inflammatory activation that support the assessment of patient prognosis and response to therapy. We propose that (i) the recent advances in fast, dynamic monitoring of inflammatory markers (e.g., cytokines) and (ii) data-dependent theoretical and computational modeling of inflammatory marker dynamics will enable the quantification of the inflammatory response, identification of optimal, disease-specific biomarkers and the design of personalized interventions to improve patient outcomes - multidisciplinary efforts in which biomedical engineers may potentially contribute. To illustrate these ideas, we describe the actions of cytokines, acute phase proteins and hormones in the inflammatory response and discuss their role in local wounds, COVID-19, cancer, autoimmune diseases, neurodegenerative diseases and aging, with a central focus on cardiac surgery. We also discuss the challenges and opportunities involved in tracking and modulating inflammation in clinical settings.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"82 ","pages":"Article 108592"},"PeriodicalIF":12.1,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guidelines toward ecologically-informed bioprospecting for microbial plastic degradation","authors":"Feng M. Cai ,&nbsp;Siqi Jiang ,&nbsp;Paul Daly ,&nbsp;Mounes Bakhshi ,&nbsp;Kai Cartwright ,&nbsp;Irina S. Druzhinina","doi":"10.1016/j.biotechadv.2025.108590","DOIUrl":"10.1016/j.biotechadv.2025.108590","url":null,"abstract":"<div><div>Biological degradation of plastics by microbial enzymes offers a sustainable alternative to traditional waste management methods that often pollute the environment. This review explores ecologically-informed bioprospecting for microorganisms possessing enzymes suitable for biological plastic waste treatment. Natural habitats enriched in plastic-like polymers, such as insect-derived polyesters, epicuticular microbial biofilms in the phyllosphere of plants in extreme environments, or aquatic ecosystems, are highlighted as promising reservoirs for bioprospecting. Anthropogenic habitats, including plastic-polluted soils and the plastisphere, have yielded potent enzymes such as PETases and cutinases, which are being exploited in biotechnology. However, bioprospecting in plastispheres and artificial environments frequently leads to the isolation of environmental opportunistic microorganisms, such as <em>Pseudomonas aeruginosa</em>, <em>Aspergillus fumigatus</em>, <em>Parengyodontium album</em>, or species of <em>Fusarium</em>, which are capable of becoming human and/or plant pathogens. These cases necessitate stringent biosecurity measures, including accurate molecular identification, ecological assessment, and containment protocols. Beyond advancing bioprospecting approaches toward a broader scope of relevant habitats, this review underscores the educational value of such screenings, specifically, in understudied natural habitats, emphasizing its potential to uncover novel enzymes and microorganisms and engage the next generation of researchers in interdisciplinary study integrating environmental microbiology, molecular biology, enzymology, polymer chemistry, and bioinformatics. Finally, we offer guidelines for microbial bioprospecting in various laboratory settings, ranging from standard environmental microbiology facilities to high-biosecurity facilities, thereby maximizing the diversity of scientists who may contribute to addressing urgent environmental challenges associated with plastic waste.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"82 ","pages":"Article 108590"},"PeriodicalIF":12.1,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on the expanding biotechnological frontier of Pedobacter","authors":"Gonçalo Figueiredo ,&nbsp;Hugo Osório ,&nbsp;Marta V. Mendes ,&nbsp;Sónia Mendo","doi":"10.1016/j.biotechadv.2025.108588","DOIUrl":"10.1016/j.biotechadv.2025.108588","url":null,"abstract":"<div><div>The genus <em>Pedobacter</em> consists of Gram-negative bacteria with a broad geographic distribution, isolated from diverse habitats, including water, soil, plants, wood, rocks and animals. However, characterization efforts have been limited to a small number of species. Likewise, in the context of natural products (NP), only a small fraction of <em>Pedobacter</em> -derived NPs have been characterized so far. In contrast, in silico analysis of the increasing number of available genomes in the databases, suggests a wealth of yet to be discovered compounds. Notable biotechnological applications described so far include the production of heparinases and chondroitinases for therapeutic purposes, phytases and galactosidases as aquaculture feed supplements, alginate lyases for biofuel production, and secondary metabolites such as pedopeptins and isopedopeptins with antimicrobial properties. Further research integrating synthetic biology approaches, holds great promise for unlocking the hidden potential of members of this genus, thus expanding its industrial applications.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"82 ","pages":"Article 108588"},"PeriodicalIF":12.1,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143881542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selecting optimal algal strains for robust photosynthetic upgrading of biogas under temperate oceanic climates","authors":"Muhammad Nabeel Haider ,&nbsp;Linda O'Higgins ,&nbsp;Richard O'Shea ,&nbsp;Lorraine Archer ,&nbsp;David M. Wall ,&nbsp;Nikita Verma ,&nbsp;María del Rosario Rodero ,&nbsp;Muhammad Aamer Mehmood ,&nbsp;Jerry D. Murphy ,&nbsp;Archishman Bose","doi":"10.1016/j.biotechadv.2025.108581","DOIUrl":"10.1016/j.biotechadv.2025.108581","url":null,"abstract":"<div><div>Biogas generated from anaerobic digestion can be upgraded to biomethane by photosynthetic biogas upgrading, using CO₂ as a bioresource for algal (cyanobacteria and microalgae) cultivation. This allows the upgrading technology to offer economic and environmental benefits to conventional physiochemical upgrading techniques (which can be energy-intensive and costly) by co-generating biomethane with high-value biomass. However, a critical challenge in implementing this technology in temperate oceanic climatic conditions (as found in Japan, and the northwest coasts of Europe and of North America, with average temperatures ranging between 5 and 20 °C) is the selection of algal strains that must be capable of sustained growth under lower ambient temperatures. Accordingly, this paper investigated the selection of algae that met seven key criteria: optimal growth at high pH (9–11); at alkalinity of 1.5–2.5 g inorganic carbon per litre; operation at low temperature (5–20 °C); tolerance to high CO₂ concentrations (above 20 %); capability for mixotrophic cultivation; ability to accumulate high-value metabolites such as photosynthetic pigments and bioactive fatty acids; and ease of harvesting. Of the twenty-six algal species assessed and ranked using a Pugh Matrix, <em>Anabaena</em> sp. and <em>Phormidium</em> sp. were assessed as the most favourable species, followed by <em>Oscillatoria</em> sp., <em>Spirulina subsalsa</em>, and <em>Leptolyngbya</em> sp. Adaptive laboratory evolution together with manipulation of abiotic factors could be effectively utilised to increase the efficiency and economic feasibility of the use of the selected strain in a photosynthetic biogas upgrading system, through improvement of growth and yield of high-value compounds.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"82 ","pages":"Article 108581"},"PeriodicalIF":12.1,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143886206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomics and plant-microbe symbioses: Microbial community dynamics, functional roles in carbon sequestration, nitrogen transformation, sulfur and phosphorus mobilization for sustainable soil health","authors":"Atif Khurshid Wani ,&nbsp;Fayzan Qadir ,&nbsp;Noureddine Elboughdiri ,&nbsp;Farida Rahayu ,&nbsp;Saefudin ,&nbsp;Dibyo Pranowo ,&nbsp;Chaireni Martasari ,&nbsp;Mia Kosmiatin ,&nbsp;Cece Suhara ,&nbsp;Tri Sudaryono ,&nbsp;Yusmani Prayogo ,&nbsp;Krishna Kumar Yadav ,&nbsp;Khursheed Muzammil ,&nbsp;Lienda Bashier Eltayeb ,&nbsp;Maha Awjan Alreshidi ,&nbsp;Reena Singh","doi":"10.1016/j.biotechadv.2025.108580","DOIUrl":"10.1016/j.biotechadv.2025.108580","url":null,"abstract":"<div><div>Biogeochemical cycles are fundamental processes that regulate the flow of essential elements such as carbon, nitrogen, and phosphorus, sustaining ecosystem productivity and global biogeochemical equilibrium. These cycles are intricately influenced by plant-microbe symbioses, which facilitate nutrient acquisition, organic matter decomposition, and the transformation of soil nutrients. Through mutualistic interactions, plants and microbes co-regulate nutrient availability and promote ecosystem resilience, especially under environmental stress. Metagenomics has emerged as a transformative tool for deciphering the complex microbial communities and functional genes driving these cycles. By enabling the high-throughput sequencing and annotation of microbial genomes, metagenomics provides unparalleled insights into the taxonomic diversity, metabolic potential, and functional pathways underlying microbial contributions to biogeochemical processes. Unlike previous reviews, this work integrates recent advancements in metagenomics with complementary omics approaches to provide a comprehensive perspective on how plant-microbe interactions modulate biogeochemical cycles at molecular, genetic, and ecosystem levels. By highlighting novel microbial processes and potential biotechnological applications, this review aims to guide future research in leveraging plant-microbe symbioses for sustainable agriculture, ecosystem restoration, and climate change mitigation.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"82 ","pages":"Article 108580"},"PeriodicalIF":12.1,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthetic biology of Fusarium for the sustainable production of valuable bioproducts","authors":"Rima Gnaim ,&nbsp;Rodrigo Ledesma-Amaro","doi":"10.1016/j.biotechadv.2025.108579","DOIUrl":"10.1016/j.biotechadv.2025.108579","url":null,"abstract":"<div><div>Synthetic biology offers transformative opportunities to optimise <em>Fusarium</em> species as efficient platforms for the sustainable production of diverse bioproducts. Advanced engineering techniques, including CRISPR/Cas9, RNA interference and synthetic promoters, have enhanced the manipulation of metabolic pathways, enabling higher yields of industrially relevant compounds. Recent insights from next-generation sequencing and omics technologies have significantly expanded our understanding of <em>Fusarium's</em> metabolic networks, leading to more precise strain engineering. Despite these advances, challenges such as metabolic bottlenecks, regulatory complexities and strain stability remain significant barriers to industrial-scale applications. The development of efficient genetic tools, together with the expansion of our knowledge of <em>Fusarium</em> physiology and genetics thanks to systems biology approaches, holds promise to unlock <em>Fusarium's</em> full potential as a sustainable cell factory. This review focuses on the genetic and metabolic tools available to enhance <em>Fusarium's</em> capacity to produce biofuels, pharmaceuticals, enzymes and other valuable compounds. It also highlights key innovations and discusses future directions for leveraging <em>Fusarium</em> as an environmentally friendly bioproduction system.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108579"},"PeriodicalIF":12.1,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological production and emerging applications of betalains: A review","authors":"Mohammad Imtiyaj Khan ,&nbsp;Guy Polturak","doi":"10.1016/j.biotechadv.2025.108576","DOIUrl":"10.1016/j.biotechadv.2025.108576","url":null,"abstract":"<div><div>Betalains are food-grade hydrophilic pigments with antioxidant and biological activities, predominantly found in plants. Betanin is a red-violet betalain synthesized from tyrosine through L-DOPA formation, its subsequent aromatic ring-opening, spontaneous cyclization to betalamic acid, and then pH-dependent condensation with i) cyclo‐DOPA-5-<em>O</em>-glucoside or ii) cyclo‐DOPA followed by 5-<em>O</em>-glucosylation. This short pathway in plants for betanin biosynthesis has been heterologously expressed in other organisms (e.g. non-betalainic plants, yeasts, and fungi) using <em>CYP76AD1</em>, <em>DOD1</em>, and <em>cDOPA5GT</em> or <em>B5GT</em>, corresponding to the enzymatic steps mentioned above. For the red-violet color formation through heterologous expression of the pathway genes in non-betalainic plants, a simplified reporter gene called <em>RUBY</em> has been developed recently. Without any systems engineering, expression of <em>RUBY</em> in non-betalainic plants resulted in accumulation of up to 203 mg betalains/100 g fresh weight of peanut leaves. In yeasts, <em>Saccharomyces cerevisiae</em> and <em>Yarrowia lipolytica</em>, and fungus <em>Fusarium venenatum</em>, betanin production has been achieved through overexpression of the pathway genes, with productivity reaching up to 0.62 mg/L/h, 26 mg/L/h, and 26.4 mg/L/h from <span>d</span>-glucose as carbon source, respectively, after considerable systems engineering and gene copy number augmentation. This review critically analyzes recent biotechnological production of betalains to highlight the advancements and strategies for improvement in the technology. Also, emerging applications of betalain biosynthetic gene products or betalains as biosensors, fluorescent probes, meat analog colors, and others are discussed to strengthen the need for systems engineering and process optimization for large-scale industrial production of these pigments.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108576"},"PeriodicalIF":12.1,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143815023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal ergot alkaloids: Metabolic pathways, biological functions, and advances in synthetic reprogramming","authors":"Wanling Jiang ,&nbsp;Xingyu Hou ,&nbsp;Guoxiong Peng ,&nbsp;Yuxian Xia ,&nbsp;Yueqing Cao","doi":"10.1016/j.biotechadv.2025.108578","DOIUrl":"10.1016/j.biotechadv.2025.108578","url":null,"abstract":"<div><div>Ergot alkaloids (EAs) are a class of secondary metabolites produced by fungi. These compounds are predominantly synthesized by Ascomycota, with variations in types and biosynthetic pathways among different fungal species. The EA synthesis has minimal impact on the normal growth and development of most EA-producing fungi, but serves as a virulence factor that influences the biocontrol functions of entomopathogenic fungi and symbiotic fungi in plants. In the medical field, EAs have been widely used for treating neurological disorders such as Parkinson's disease. However, the biosynthetic pathways of EAs are highly complex and significantly influenced by environmental factors, resulting in low yields from field production or chemical synthesis. To address the global demand for EAs, various strategies have been developed to reprogram the biosynthetic pathways in some chassis strains, aiming to simplify the process and increase EA production. This review summarizes the biosynthetic pathways and regulatory mechanisms of EAs in fungi, their biological functions, and recent advances in strategies for synthetic reprogramming.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108578"},"PeriodicalIF":12.1,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyethylene biodegradation: A multifaceted approach","authors":"Anjali Purohit ,&nbsp;Bastien Cochereau ,&nbsp;Omprakash Sarkar ,&nbsp;Ulrika Rova ,&nbsp;Paul Christakopoulos ,&nbsp;Io Antonopoulou ,&nbsp;Silas Villas-Boas ,&nbsp;Leonidas Matsakas","doi":"10.1016/j.biotechadv.2025.108577","DOIUrl":"10.1016/j.biotechadv.2025.108577","url":null,"abstract":"<div><div>The inert nature, durability, low cost, and wide applicability of plastics have made this material indispensable in our lives. This dependency has resulted in a growing number of plastic items, of which a substantial part is disposed in landfills or dumped in the environment, thereby affecting terrestrial and aquatic ecosystems. Among plastic materials, polyolefins are the most abundant and are impervious to biodegradation, owing to the presence of strong C<img>C and C<img>H bonds. Nevertheless, naturally occurring biodegradation of polyolefins, albeit limited, has been reported. This observation has sparked research on microbial polyolefin degradation. More efficient and targeted versions of this process could be developed also in the laboratory by designing synthetic microbial consortia with engineered enzymes. In this review, we discuss strategies for the development of such microbial consortia and identification of novel polyolefin-degrading microorganisms, as well as the engineering of polyethylene-oxidizing enzymes with greater catalytic efficacy. Finally, different techniques for the design of synthetic microbial consortia capable of successful polyolefin bioremediation will be outlined.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"82 ","pages":"Article 108577"},"PeriodicalIF":12.1,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143787693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taking the 3Rs to a higher level: replacement and reduction of animal testing in life sciences in space research","authors":"Mathieu Vinken ,&nbsp;Daniela Grimm ,&nbsp;Sarah Baatout ,&nbsp;Bjorn Baselet ,&nbsp;Afshin Beheshti ,&nbsp;Markus Braun ,&nbsp;Anna Catharina Carstens ,&nbsp;James A. Casaletto ,&nbsp;Ben Cools ,&nbsp;Sylvain V. Costes ,&nbsp;Phoebe De Meulemeester ,&nbsp;Bartu Doruk ,&nbsp;Sara Eyal ,&nbsp;Miguel J.S. Ferreira ,&nbsp;Silvana Miranda ,&nbsp;Christiane Hahn ,&nbsp;Sinem Helvacıoğlu Akyüz ,&nbsp;Stefan Herbert ,&nbsp;Dmitriy Krepkiy ,&nbsp;Yannick Lichterfeld ,&nbsp;Danilo A. Tagle","doi":"10.1016/j.biotechadv.2025.108574","DOIUrl":"10.1016/j.biotechadv.2025.108574","url":null,"abstract":"<div><div>Human settlements on the Moon, crewed missions to Mars and space tourism will become a reality in the next few decades. Human presence in space, especially for extended periods of time, will therefore steeply increase. However, despite more than 60 years of spaceflight, the mechanisms underlying the effects of the space environment on human physiology are still not fully understood. Animals, ranging in complexity from flies to monkeys, have played a pioneering role in understanding the (patho)physiological outcome of critical environmental factors in space, in particular altered gravity and cosmic radiation. The use of animals in biomedical research is increasingly being criticized because of ethical reasons and limited human relevance. Driven by the 3Rs concept, calling for replacement, reduction and refinement of animal experimentation, major efforts have been focused in the past decades on the development of alternative methods that fully bypass animal testing or so-called new approach methodologies. These new approach methodologies range from simple monolayer cultures of individual primary or stem cells all up to bioprinted 3D organoids and microfluidic chips that recapitulate the complex cellular architecture of organs. Other approaches applied in life sciences in space research contribute to the reduction of animal experimentation. These include methods to mimic space conditions on Earth, such as microgravity and radiation simulators, as well as tools to support the processing, analysis or application of testing results obtained in life sciences in space research, including systems biology, live-cell, high-content and real-time analysis, high-throughput analysis, artificial intelligence and digital twins. The present paper provides an in-depth overview of such methods to replace or reduce animal testing in life sciences in space research.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"81 ","pages":"Article 108574"},"PeriodicalIF":12.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}