Biotechnology advances最新文献_第4页

Chimeric enzymes in the pulp and paper making industry: Current developments 纸浆和造纸工业中的嵌合酶：最新进展

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-02-09 DOI: 10.1016/j.biotechadv.2025.108530

Abdullah Arsalan , Yuvaraj Ravikumar , Xinrui Tang , Zijing Cao , Mei Zhao , Wenjing Sun , Xianghui Qi

{"title":"Chimeric enzymes in the pulp and paper making industry: Current developments","authors":"Abdullah Arsalan , Yuvaraj Ravikumar , Xinrui Tang , Zijing Cao , Mei Zhao , Wenjing Sun , Xianghui Qi","doi":"10.1016/j.biotechadv.2025.108530","DOIUrl":"10.1016/j.biotechadv.2025.108530","url":null,"abstract":"<div><div>The pulp and paper (P&P) industry plays a vital role in supporting the social and economic progress of a country by supplying essential commodities. Conventional P&P processing often consumes significant energy and use chemical agents to produce hazardous intermediates. The use of enzymes in the P&P industry has significantly reduced both the chemical and energy demands during processing. A variety of enzyme combination cocktails are used to perform multiple functions in a single step, but often fail to operate synergistically because of significant differences in operational conditions. This lack of synergy under various operating conditions highlights the need for engineered chimeric enzymes. Moreover, enzyme engineering approaches enable enzymes to perform catalysis in sub-optimal environment. Enzymes have been engineered to improve their catalytic properties and enhance operational stability. Designing multifunctional or chimeric enzymes can function simultaneously across diverse operational conditions. Chimeric enzymes enable effective synergistic action of multiple enzymes in the P&P industry. This review aims to provide clear insights into the selective development of chimeric enzymes using enzyme engineering approaches for their effective use in the P&P industry.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"79 ","pages":"Article 108530"},"PeriodicalIF":12.1,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Post-translational assembly of multi-functional antibody 多功能抗体的翻译后组装。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-02-08 DOI: 10.1016/j.biotechadv.2025.108533

Baizhen Gao , Qing Sun

{"title":"Post-translational assembly of multi-functional antibody","authors":"Baizhen Gao , Qing Sun","doi":"10.1016/j.biotechadv.2025.108533","DOIUrl":"10.1016/j.biotechadv.2025.108533","url":null,"abstract":"<div><div>The advent of multi-specific antibodies has introduced a significant advantage over traditional monoclonal antibody therapeutics by engaging multiple targets and pathways. This review delves into the post-translational assembly techniques for multi-specific antibodies, highlighting the innovations and challenges associated with approaches of chemical conjugation, oligonucleotide-mediated assembly, and protein-protein interactions. Chemical conjugation methods have evolved to enhance the assembly process's specificity and flexibility, enabling transient engagement and versatile antibody formats. Meanwhile, oligonucleotide-mediated assembly leverages the precision of Watson–Crick base pairing, granting unmatched control over the antibody's structure and functional orientation. Additionally, protein-protein interaction strategies, notably through SpyTag/SpyCatcher systems, present a direct assembly approach without necessitating ancillary modifications, streamlining the production process. This review summarizes the significance of these methodologies in generating antibodies with diverse structures and multi-target engagement capabilities, underscoring their potential in improving therapeutic efficacy and reducing production complexity.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"80 ","pages":"Article 108533"},"PeriodicalIF":12.1,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143390059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational advances in biosynthetic gene cluster discovery and prediction 生物合成基因簇发现与预测的计算研究进展。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-02-07 DOI: 10.1016/j.biotechadv.2025.108532

Sisi Zhu , Hongquan Xu , Yuhong Liu , Yanfeng Hong , Haowen Yang , Changli Zhou , Lin Tao

引用次数: 0

Polyhydroxyalkanoates production from microalgae for sustainable bioplastics: A review 微藻生产聚羟基烷酸酯（PHA）用于可持续生物塑料研究进展

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-02-06 DOI: 10.1016/j.biotechadv.2025.108529

Syarifa Ilhami , Siti Nur Syaza Abdul Rahman , Muhammad Iqhrammullah , Zhafran Hamid , Yee Ho Chai , Man Kee Lam

{"title":"Polyhydroxyalkanoates production from microalgae for sustainable bioplastics: A review","authors":"Syarifa Ilhami , Siti Nur Syaza Abdul Rahman , Muhammad Iqhrammullah , Zhafran Hamid , Yee Ho Chai , Man Kee Lam","doi":"10.1016/j.biotechadv.2025.108529","DOIUrl":"10.1016/j.biotechadv.2025.108529","url":null,"abstract":"<div><div>Microalgae have emerged as a promising and sustainable source for polyhydroxyalkanoates (PHA), which are increasingly recognized for their potential in bioplastics production. However, the widespread application of microalgae-derived PHA faces challenges related to economic feasibility and scalability. This review provides a comprehensive analysis of recent advancements in the cultivation and optimization of microalgae for PHA production, highlighting the critical role of nutrient limitation, particularly nitrogen and phosphorus, in enhancing PHA accumulation. This review also explores the effectiveness of various cultivation systems, including autotrophic, heterotrophic, and mixotrophic approaches, in maximizing PHA yields. Environmental factors such as light intensity, salinity, and pH are examined for their influence on PHA synthesis pathways. Additionally, it identifies key technical and economic challenges that must be addressed to commercialize microalgae-based bioplastics to fully harness the potential of microalgae in sustainable bioplastic production.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"79 ","pages":"Article 108529"},"PeriodicalIF":12.1,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heparin and heparin-like modifications in hemodialysis membranes: Current innovations and future directions 血液透析膜中的肝素和肝素样修饰：当前的创新和未来的方向。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-02-06 DOI: 10.1016/j.biotechadv.2025.108527

Putu Teta Prihartini Aryanti , Febrianto Adi Nugroho , Yudith Yunia Kusmala

{"title":"Heparin and heparin-like modifications in hemodialysis membranes: Current innovations and future directions","authors":"Putu Teta Prihartini Aryanti , Febrianto Adi Nugroho , Yudith Yunia Kusmala","doi":"10.1016/j.biotechadv.2025.108527","DOIUrl":"10.1016/j.biotechadv.2025.108527","url":null,"abstract":"<div><div>Heparinized hemodialysis membranes represent a significant advancement in improving the biocompatibility and anticoagulant properties of dialysis treatments. This review explores the current challenges and innovations in developing these membranes, focusing on the incorporation of heparin and heparin-like substances to reduce protein adsorption, platelet adhesion, and clot formation. The methods for heparin immobilization, including covalent bonding, layer-by-layer assembly, and blending, offer promising results in enhancing membrane performance. However, issues such as long-term stability, large-scale production, and cost-effectiveness remain critical barriers to their widespread adoption. The review also highlights the role of surface activation techniques and nanotechnology in improving the functionality of heparinized membranes. Advanced methods like plasma treatment and polymer grafting provide better heparin attachment, while nanomaterial integration allows for improved blood compatibility and controlled heparin release. Despite these innovations, challenges such as heparin degradation, uneven coating, and the complexity of scaling up remain unresolved. Future research should focus on optimizing heparin distribution, enhancing durability, and making the production process more cost-efficient. This paper outlines potential interdisciplinary approaches, such as bioinspired materials and nanotechnology applications, to address these challenges and pave the way for next-generation hemodialysis membranes that are safer, more effective, and more accessible.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"80 ","pages":"Article 108527"},"PeriodicalIF":12.1,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unleashing the potential of microbial biosynthesis of monoterpenes via enzyme and metabolic engineering 利用酶和代谢工程释放微生物合成单萜烯的潜力。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-02-05 DOI: 10.1016/j.biotechadv.2025.108525

Dianqi Yang , Hong Liang , Xuxu Li, Chenyu Zhang, Zewei Lu, Xiaoqiang Ma

{"title":"Unleashing the potential of microbial biosynthesis of monoterpenes via enzyme and metabolic engineering","authors":"Dianqi Yang , Hong Liang , Xuxu Li, Chenyu Zhang, Zewei Lu, Xiaoqiang Ma","doi":"10.1016/j.biotechadv.2025.108525","DOIUrl":"10.1016/j.biotechadv.2025.108525","url":null,"abstract":"<div><div>Monoterpenes (MTPs) are valuable isoprenoids widely used in cosmetics, food flavorings, pharmaceuticals, etc. Compared to plant extraction and chemical synthesis, microbial biosynthesis offers superior sustainability and efficiency in producing natural MTPs, overcoming the limitations of raw material dependency, environmental impact, and racemic mixtures inherent in these methods. This review comprehensively discusses the development of natural or non-natural biosynthetic pathways for producing regular and irregular MTPs, emphasizing the importance of enzyme and metabolic engineering to optimize monoterpene synthases (MTPSs) in various engineered microbial cell factories (MCFs). The advances in functional expression of MTPS to enhance enzyme activity, substrate channeling of MTPS with critical biosynthesis enzymes, protein engineering of MTPS, targeted localization of MTPS in the subcellular organelle, and other favorable engineering strategies are discussed in detail. Leveraging these technologies, the engineered microbes will achieve the production of the defined product profile with higher titer/yield/productivity and improved industrial adaptability. Furthermore, we highlight the important development direction for optimizing MTPS performance and biosynthetic pathways, ensuring the microbial production of natural MTPs in a more efficient and application-specific manner.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"79 ","pages":"Article 108525"},"PeriodicalIF":12.1,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143373637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the full potential of plant cell-based production for valuable proteins: Challenges and innovative strategies 释放植物细胞生产有价值蛋白质的全部潜力：挑战和创新策略

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-02-04 DOI: 10.1016/j.biotechadv.2025.108526

Jianfeng Xu , Paula PerezSanchez , Shekoofeh Sadravi

{"title":"Unlocking the full potential of plant cell-based production for valuable proteins: Challenges and innovative strategies","authors":"Jianfeng Xu , Paula PerezSanchez , Shekoofeh Sadravi","doi":"10.1016/j.biotechadv.2025.108526","DOIUrl":"10.1016/j.biotechadv.2025.108526","url":null,"abstract":"<div><div>Plant cell-based bioproduction systems offer a promising platform for the sustainable production of valuable proteins as they provide distinctive advantages over mammalian cell culture and whole plant cultivation. However, significant technical challenges remain, including low productivity, altered efficacy of plant-derived proteins, along with issues in culture process development, such as cell clumping, genetic instability, and difficulties with cryopreservation. To date, the full production potential of this platform remains largely untapped. This review addresses these critical challenges and proposes innovative strategies to unlock the full potential of the production platform. Rather than simply revisiting past advancements or summarizing current progress, it proposes forward-thinking solutions with a particular emphasis on cellular engineering. Key strategies include designing novel protein partners to enhance recombinant protein accumulation and functionality, employing precise gene integration techniques in genome to enhance transgene transcription, implementing cutting-edge methods for screening and maintaining elite cell lines to mitigate genetic instability, and leveraging genome editing tools for cellular engineering to develop new plant cell lines optimized for bioproduction. A key focus is on cell wall engineering to develop cellulose- or pectin-deficient cell lines, facilitating modifications to the morphology of existing plant cell lines. By exploring these innovative approaches, this review aims to foster innovative thinking and inspire future research in plant cell-based bioproduction.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"79 ","pages":"Article 108526"},"PeriodicalIF":12.1,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143277107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular variability as a driver for bioprocess innovation and optimization 细胞变异性作为生物过程创新和优化的驱动因素

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-02-04 DOI: 10.1016/j.biotechadv.2025.108528

M. Eigenfeld , S.P. Schwaminger

{"title":"Cellular variability as a driver for bioprocess innovation and optimization","authors":"M. Eigenfeld , S.P. Schwaminger","doi":"10.1016/j.biotechadv.2025.108528","DOIUrl":"10.1016/j.biotechadv.2025.108528","url":null,"abstract":"<div><div>Cellular heterogeneity plays a crucial role in biotechnological processes, significantly influencing metabolic activity, product yield, and process consistency. This review explores the different dimensions of cellular heterogeneity, focusing on its manifestation at both single-cell and population levels. The study examines how factors such as asymmetric cell division, age, and environmental conditions contribute to functional diversity within cell populations, with an emphasis on microorganisms like yeast. Age-related cellular heterogeneity, in particular, is highlighted for its impact on metabolic pathways, mitochondrial function, and secondary metabolite production, which directly affect bioprocess outcomes. Furthermore, the review discusses advanced techniques for detecting and managing heterogeneity, including surface marker-based approaches, which utilize proteins, polysaccharides, and lipids, and label-free methods that leverage cellular volume and physical properties for separation. Understanding and controlling cellular heterogeneity is essential for optimizing industrial bioprocesses, improving yield, and ensuring product quality. The review also underscores the potential of emerging biotechnological tools, such as real-time single-cell analysis and microfluidic devices, in enhancing separation techniques and managing cellular diversity for better process efficiency and robustness.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"79 ","pages":"Article 108528"},"PeriodicalIF":12.1,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143348428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards the application of nature's catalytic nanomachines: Cellulosomes in 2nd generation biofuel production

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-01-31 DOI: 10.1016/j.biotechadv.2025.108523

Maša Vodovnik , Nataša Lindič

{"title":"Towards the application of nature's catalytic nanomachines: Cellulosomes in 2nd generation biofuel production","authors":"Maša Vodovnik , Nataša Lindič","doi":"10.1016/j.biotechadv.2025.108523","DOIUrl":"10.1016/j.biotechadv.2025.108523","url":null,"abstract":"<div><div>Cellulosomes are highly efficient, complex multi-enzyme assemblies, predominantly found in anaerobic bacteria, which offer substantial potential for second-generation biofuel production through efficient lignocellulosic biomass degradation, thus reducing the need for costly pretreatments. Recent advances in cellulosome research have significantly contributed to developing more efficient consolidated bioprocessing (CBP) platforms for biofuel production. This review highlights the latest progress in designing cellulosomes for optimized enzyme synergy and substrate specificity, as well as advances in engineering cellulosome-producing whole-cell biocatalysts tailored for biofuel applications. Apart from recombinant approaches to the development of CBP platforms, metabolic engineering of cellulosome-producing strains (CPS) and co-culture systems that combine CPS with solvent-producing microbes are also discussed. Current challenges and future directions are outlined that emphasize the role of cellulosomes as powerful tools in advancing the efficiency of lignocellulosic biorefineries.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"79 ","pages":"Article 108523"},"PeriodicalIF":12.1,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidation and biosynthesis of tetrahydroisoquinoline alkaloids: Recent advances and prospects

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2025-01-28 DOI: 10.1016/j.biotechadv.2025.108524

Yue Gao , Fei Li , Zhenbo Yuan , Zhengshan Luo , Yijian Rao

引用次数: 0