Biotechnology advances最新文献

筛选
英文 中文
Engineering cyclized biomolecules for advanced diagnostic and therapeutic applications 工程循环生物分子用于先进的诊断和治疗应用
IF 12.5 1区 工程技术
Biotechnology advances Pub Date : 2025-08-22 DOI: 10.1016/j.biotechadv.2025.108694
Yeonju Lee , Bohyun Oh , Sumin Kang , Sumin Kim , Young-Pil Kim
{"title":"Engineering cyclized biomolecules for advanced diagnostic and therapeutic applications","authors":"Yeonju Lee ,&nbsp;Bohyun Oh ,&nbsp;Sumin Kang ,&nbsp;Sumin Kim ,&nbsp;Young-Pil Kim","doi":"10.1016/j.biotechadv.2025.108694","DOIUrl":"10.1016/j.biotechadv.2025.108694","url":null,"abstract":"<div><div>Nucleic acids, peptides, and proteins demonstrate remarkable structural diversity and play essential roles in various biological processes. These biomolecules function as ligands, diagnostic agents, and therapeutic cargoes. However, their practical applications are often limited by inherent instability and inefficient delivery for targeted diagnosis and therapy. Cyclization has emerged as a promising solution, endowing these biomolecules with enhanced conformational rigidity, resistance to degradation, and a broader range of biological activities. Cyclic architectures not only enhance antitumor, anti-inflammatory, and anti-infective properties, but also eliminate the need for auxiliary carriers in some applications. This deliberate cyclization further enables precise control over binding affinity, stability, and membrane permeability. In this review, we cover diverse engineering methods for creating cyclized biomolecules and explore their applications in biosensing, targeted imaging, and delivery. Moving beyond naturally occurring forms, we highlight rationally engineered cyclized constructs that substantially expand their theranostic landscape in biology and biotechnology.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"84 ","pages":"Article 108694"},"PeriodicalIF":12.5,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144900411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multidimensional strategies for efficient heterologous protein expression in Aspergillus niger 黑曲霉高效异源蛋白表达的多维策略
IF 12.5 1区 工程技术
Biotechnology advances Pub Date : 2025-08-20 DOI: 10.1016/j.biotechadv.2025.108690
Zehan Shi , Jiaxuan Li , Fengmei Zhu , Xiaojing Liu , Jiefang Zhou , Jun Li , Wentao Xu
{"title":"Multidimensional strategies for efficient heterologous protein expression in Aspergillus niger","authors":"Zehan Shi ,&nbsp;Jiaxuan Li ,&nbsp;Fengmei Zhu ,&nbsp;Xiaojing Liu ,&nbsp;Jiefang Zhou ,&nbsp;Jun Li ,&nbsp;Wentao Xu","doi":"10.1016/j.biotechadv.2025.108690","DOIUrl":"10.1016/j.biotechadv.2025.108690","url":null,"abstract":"<div><div>Microbial protein is a promising alternative to animal and plant proteins. <em>Aspergillus niger</em>, a generally recognized as safe (GRAS) microorganism, is frequently used for heterologous protein production, although its expression efficiency is constrained by multiple factors, including gene transcription, metabolic flux distribution, protein folding, and secretion pathways. However, constructing universal <em>Aspergillus niger</em> chassis cells for efficient protein production remains challenging due to the diverse properties of different proteins. With advancements in synthetic biology, numerous molecular biology tools and metabolic engineering strategies have been employed to address these issues. This article summarizes and discusses the latest progress in enhancing heterologous protein production from five dimensions: expression systems, secretion pathways, metabolic flux, intelligent fermentation, and systematic optimization through multi-omics integration. Additionally, it prospects the efficient and sustainable production of heterologous proteins by <em>Aspergillus niger</em>.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"84 ","pages":"Article 108690"},"PeriodicalIF":12.5,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phage therapy against Klebsiella pneumoniae: An evolving perspective 噬菌体治疗肺炎克雷伯菌:一个不断发展的观点
IF 12.5 1区 工程技术
Biotechnology advances Pub Date : 2025-08-19 DOI: 10.1016/j.biotechadv.2025.108689
Jiabao Xing , Hang Zhang , Leyin Zheng , Jinxin Zhao , Yuying Zhang , Zhiying Xu , Yajun Zhai , Gongzheng Hu , Jian Li , Hua Wu
{"title":"Phage therapy against Klebsiella pneumoniae: An evolving perspective","authors":"Jiabao Xing ,&nbsp;Hang Zhang ,&nbsp;Leyin Zheng ,&nbsp;Jinxin Zhao ,&nbsp;Yuying Zhang ,&nbsp;Zhiying Xu ,&nbsp;Yajun Zhai ,&nbsp;Gongzheng Hu ,&nbsp;Jian Li ,&nbsp;Hua Wu","doi":"10.1016/j.biotechadv.2025.108689","DOIUrl":"10.1016/j.biotechadv.2025.108689","url":null,"abstract":"<div><div><em>Klebsiella pneumoniae</em> represents one of the most concerning ESKAPE pathogens, with multidrug-resistant strains driving urgent clinical interest in phage therapy as a viable alternative to antibiotics. However, the evolutionary arms race between phages and bacteria has equipped <em>K. pneumoniae</em> with sophisticated anti-phage immune defenses, posing a substantial barrier to durable therapeutic success. Through systematic analysis of <em>K. pneumoniae</em>-phage co-evolutionary dynamics, we identify predominant resistance mechanisms and discuss why these mechanisms primarily concentrate on adsorption blocking pathways. We then integrate clinical case studies with preclinical research to evaluate combination strategies against phage resistance, particularly highlighting synergistic approaches using antibiotics-phage or phage cocktails/phage serial therapy that increase selective pressure while reducing bacterial host adaptability and pathogenicity. Finally, we propose a computational roadmap leveraging machine learning for phage characterization, host-interaction prediction and <em>de novo</em> genome engineering, with particular emphasis on minimizing resistance emergence. This interdisciplinary review provides both immediate clinical guidance and a forward-looking vision for rational phage design, applicable beyond not only to <em>K. pneumoniae</em> but also to other high-priority pathogens. We also highlight that integrations of synthetic biology, computational science, and microbiology will be essential for transitioning phage therapy from experimental treatments to standardized interventions addressing antimicrobial resistance.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"84 ","pages":"Article 108689"},"PeriodicalIF":12.5,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144878249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advances in molecular tools for parasitic nematodes of animals – From genetic markers to metabarcoding and genomics 动物寄生线虫分子工具研究进展——从遗传标记到元条形码和基因组学。
IF 12.5 1区 工程技术
Biotechnology advances Pub Date : 2025-08-12 DOI: 10.1016/j.biotechadv.2025.108688
Robin B. Gasser
{"title":"Advances in molecular tools for parasitic nematodes of animals – From genetic markers to metabarcoding and genomics","authors":"Robin B. Gasser","doi":"10.1016/j.biotechadv.2025.108688","DOIUrl":"10.1016/j.biotechadv.2025.108688","url":null,"abstract":"<div><div>Parasitic nematodes are globally important pathogens of animals, humans and plants. The accurate identification and characterisation of these nematodes are essential for diagnostics, systematics and epidemiological surveillance. Over the past decades, molecular technologies have progressed from targeted PCR-based assays using nuclear ribosomal markers – particularly the first and second internal transcribed spacers (ITS-1 and ITS-2) – to high-throughput metabarcoding and genome-enabled approaches. These advances have transformed species discovery, facilitated the detection of cryptic taxa and supported the development of sensitive diagnostic tools for parasitic nematodes of veterinary, medical and agricultural importance. This review traces the progression of molecular technologies used to study parasitic nematodes, with emphasis on the definition and validation of ribosomal and mitochondrial DNA markers, metabarcoding frameworks, such as the “nemabiome”, and the expanding availability of genomic resources. Particular attention is given to the emergence of workflows and bioinformatic pipelines supporting primer evaluation, variant detection, taxonomic assignment and analyses of genetic diversity. The integration of curated reference databases, multi-marker strategies and advanced informatics has allowed for scalable, reproducible and high-resolution investigations of nematode diversity. Recent innovations in long-read sequencing and CRISPR-based enrichment, which may enable amplification-free, multi-locus profiling of nematode communities, are also highlighted. Collectively, these advances in molecular and genomic technologies – underpinned by reproducible informatic frameworks – are transforming the systematics, diagnostics and epidemiological surveillance of parasitic nematodes of animals.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"85 ","pages":"Article 108688"},"PeriodicalIF":12.5,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144854396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Understanding low-phosphate stress responses in plants: Opportunities for genome editing to improve phosphorous use efficiency (PUE) 了解植物低磷胁迫反应:基因组编辑提高磷利用效率(PUE)的机会
IF 12.5 1区 工程技术
Biotechnology advances Pub Date : 2025-08-11 DOI: 10.1016/j.biotechadv.2025.108686
Stanislaus Antony Ceasar , Heba T. Ebeed , Muthusamy Ramakrishnan , Pedro García-Caparrós , Savarimuthu Ignacimuthu
{"title":"Understanding low-phosphate stress responses in plants: Opportunities for genome editing to improve phosphorous use efficiency (PUE)","authors":"Stanislaus Antony Ceasar ,&nbsp;Heba T. Ebeed ,&nbsp;Muthusamy Ramakrishnan ,&nbsp;Pedro García-Caparrós ,&nbsp;Savarimuthu Ignacimuthu","doi":"10.1016/j.biotechadv.2025.108686","DOIUrl":"10.1016/j.biotechadv.2025.108686","url":null,"abstract":"<div><div>Phosphorus (P) is a critical macronutrient essential for plant growth, yet its availability in soil is often limited due to poor mobility and fixation with metal ions due to acidic or alkaline soils. Plants have evolved complex adaptive responses to overcome phosphate (Pi) deficiency. Recent advancements in genome editing, particularly CRISPR/Cas tools, offer opportunities to enhance these adaptive traits for sustainable agriculture. This review consolidates current understanding of low Pi stress signaling pathways, including morphological (root architecture changes), biochemical (hormone regulation, lipid modification, organic acid exudation), and molecular (transcription factors (TFs), phosphate transporters, and microRNAs), and identifies prime candidate genes for genome editing applications. Key regulators such as phosphate transporter (PHT, PHO1), TFs (PHR1, WRKYs) and microRNAs (miR399/827) manage Pi uptake, redistribution, and signaling. Genome editing strategies targeting root-specific traits, hormonal integration, lipid remodeling, and transcriptional regulation are discussed as viable ways for improving phosphorous use efficiency (PUE). Harnessing CRISPR/Cas tools can lead to the development of crops with optimized PUE, reduced dependency on synthetic fertilizers, and improved adaptability to Pi-deficient soils. The review provides a comprehensive roadmap for researchers and breeders to apply CRISPR/Cas technology toward building next-generation crops capable of thriving under low Pi conditions.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"84 ","pages":"Article 108686"},"PeriodicalIF":12.5,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144830143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Harnessing biological nitrogen fixation: Multi-scale engineering for self-sustaining agroecosystems 利用生物固氮:自我维持农业生态系统的多尺度工程
IF 12.5 1区 工程技术
Biotechnology advances Pub Date : 2025-08-11 DOI: 10.1016/j.biotechadv.2025.108687
Wanjing Wu, Haiyang Hu, Weiwei Wang, Ping Xu, Hongzhi Tang
{"title":"Harnessing biological nitrogen fixation: Multi-scale engineering for self-sustaining agroecosystems","authors":"Wanjing Wu,&nbsp;Haiyang Hu,&nbsp;Weiwei Wang,&nbsp;Ping Xu,&nbsp;Hongzhi Tang","doi":"10.1016/j.biotechadv.2025.108687","DOIUrl":"10.1016/j.biotechadv.2025.108687","url":null,"abstract":"<div><div>Certain prokaryotic microorganisms possess the extraordinary ability to convert atmospheric nitrogen gas into ammonia in a process known as biological nitrogen fixation. Harnessing this process as a substitute for chemical nitrogen fertilizers offers substantial benefits for agricultural productivity. Improving the efficiency of nitrogen fixation and enabling crops to fix nitrogen biologically are crucial research goals. This review explores molecular mechanisms governing nitrogenase activity, engineering strategies for enhancing nitrogen fixation efficiency in non-diazotrophic hosts, and evaluates synthetic biology approaches for establishing robust nitrogen-fixing systems. We emphasize the integration of multi-scale engineering – from nitrogen fixation circuit design in microbial chassis to rhizosphere microbiome reprogramming to engineered nitrogen-fixing crops and field applications at the ecosystem level – as critical pathways toward creating self-sustaining agroecosystems. We conclude by discussing key challenges and identifying research priorities to expand the practical applications of biological nitrogen fixation. This review provides a framework for developing multi-layered bioengineering solutions that enhance crop productivity while reducing ecological impacts, ultimately advancing the realization of self-regulating agricultural ecosystems.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"84 ","pages":"Article 108687"},"PeriodicalIF":12.5,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144842595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bio-upcycling PET waste: Advances in enzymatic hydrolysis and biosynthesis of value-added products PET废弃物的生物升级利用:酶解和生物合成增值产品的研究进展。
IF 12.5 1区 工程技术
Biotechnology advances Pub Date : 2025-08-09 DOI: 10.1016/j.biotechadv.2025.108685
Yu Zhou , Jiaxing Zhang , Shengping You , Congqiang Zhang , Wei Qi
{"title":"Bio-upcycling PET waste: Advances in enzymatic hydrolysis and biosynthesis of value-added products","authors":"Yu Zhou ,&nbsp;Jiaxing Zhang ,&nbsp;Shengping You ,&nbsp;Congqiang Zhang ,&nbsp;Wei Qi","doi":"10.1016/j.biotechadv.2025.108685","DOIUrl":"10.1016/j.biotechadv.2025.108685","url":null,"abstract":"<div><div>With excellent mechanical properties and chemical stability, poly (ethylene terephthalate) (PET), an engineering plastic, is widely applied in textiles and packaging. However, the widespread use and low biodegradability of PET have resulted in significant environmental pollution. Recent advances in PET hydrolase discovery and engineering have driven the rapid advancement of PET bio-recycling, while efficient PET hydrolases can depolymerize PET into monomers under mild conditions, providing a sustainable approach to potentially addressing the plastic pollution issue. However, PET enzymatic hydrolysis still faces some technical challenges, such as poor stability of the hydrolases and low efficiency in degrading high-crystalline PET. Thus, this review summarizes recent advances in strategies to enhance the efficiency of PET enzymatic hydrolysis and explores the interplay of factors affecting PET hydrolysis efficiency. Furthermore, we highlight the progress in metabolic engineering approaches for the biotransformation of PET degradation products into higher value chemicals, providing insights into achieving efficient PET bio-recycling. This review systematically integrates key factors for enhancing the PET enzymatic hydrolysis efficiency and showcases successful examples of PET waste further valorization, providing valuable references and insights for the industrialization of PET bio-upcycling.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"84 ","pages":"Article 108685"},"PeriodicalIF":12.5,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Specialized pro-resolving mediators: Biosynthetic pathways, biocatalytic synthesis, and applications 专门的促分解介质:生物合成途径,生物催化合成及其应用。
IF 12.5 1区 工程技术
Biotechnology advances Pub Date : 2025-08-07 DOI: 10.1016/j.biotechadv.2025.108682
Tae-Eui Lee , Kyung-Chul Shin , Jin-Byung Park , Deok-Kun Oh
{"title":"Specialized pro-resolving mediators: Biosynthetic pathways, biocatalytic synthesis, and applications","authors":"Tae-Eui Lee ,&nbsp;Kyung-Chul Shin ,&nbsp;Jin-Byung Park ,&nbsp;Deok-Kun Oh","doi":"10.1016/j.biotechadv.2025.108682","DOIUrl":"10.1016/j.biotechadv.2025.108682","url":null,"abstract":"<div><div>Specialized pro-resolving mediators (SPMs), a class of di- and trihydroxy fatty acids derived from C20- and C22-polyunsaturated fatty acids, are endogenously produced by human M2 macrophages, polymorphonuclear leukocytes, and other immune and structural cells. They play crucial roles in the resolution of inflammation and infection. Owing to their potent bioactivities, SPMs have attracted considerable interest for applications in food, cosmetics, and clinical therapeutics. Therefore, achieving high concentrations of these mediators is essential. This review provides a comprehensive overview of the nomenclature, classification, biological functions, biosynthetic pathways, and recent advances in the biocatalytic synthesis of SPMs, including lipoxins, resolvins, protectins, and maresins. We examine the biosynthetic pathways: from arachidonic acid to lipoxins; from eicosapentaenoic acid, docosahexaenoic acid, and n-3 docosapentaenoic acid to resolvin E, D, and T series, respectively; and from docosahexaenoic acid to protectins, and maresins. These pathways are catalyzed by fatty acid oxygenases, such as lipoxygenases (LOXs), aspirin-triggered cyclooxygenases, and cytochrome P450 monooxygenases, in combination with hydrolases and peroxidases. Recent advances in microbial biocatalysis, particularly through the use of recombinant cells expressing microbial LOXs, have enabled the efficient synthesis of SPMs. The discovery of microbial double‑oxygenating LOXs has significantly improved production yields, achieving gram-per-liter scale. To further enhance the biocatalytic synthesis, this review discusses enzyme discovery, protein engineering, and biocatalysis optimization strategies aimed at enhancing SPM production. Notably, computational and artificial intelligence-driven approaches are emerging as powerful tools for the discovery and engineering of high-efficiency LOXs, providing a promising route to improve the biocatalytic synthesis of SPMs.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"84 ","pages":"Article 108682"},"PeriodicalIF":12.5,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advancing approaches to cultivate industrially and ecologically relevant microorganisms from termite guts 推进从白蚁肠道中培养工业和生态相关微生物的方法。
IF 12.5 1区 工程技术
Biotechnology advances Pub Date : 2025-08-05 DOI: 10.1016/j.biotechadv.2025.108676
Farhan Ahmad , Kasun H. Bodawatta , Michael Poulsen , Daochen Zhu
{"title":"Advancing approaches to cultivate industrially and ecologically relevant microorganisms from termite guts","authors":"Farhan Ahmad ,&nbsp;Kasun H. Bodawatta ,&nbsp;Michael Poulsen ,&nbsp;Daochen Zhu","doi":"10.1016/j.biotechadv.2025.108676","DOIUrl":"10.1016/j.biotechadv.2025.108676","url":null,"abstract":"<div><div>The termite gut harbours a remarkably dense and diverse consortium of symbiotic microbes, encompassing archaeal, bacterial, and eukaryotic taxa. These symbiotic communities hold intricate ecological processes and a pronounced potential for exploitation across multifaceted domains, including industrially important enzymes, biofuels, pharmaceuticals, and bioremediation. Despite the conspicuous richness, a substantial portion of microbial assemblages inhabiting the termite gut remains undiscovered and inadequately characterized. Although traditional culture-based and culture-independent molecular-based technologies are broadly used to study termite gut microbiota, they more recent frequently encounter limitations in the isolation, culturing, and characterization of less prevalent microbial lineages and are biased toward certain taxa. The rapid development of molecular techniques has greatly promoted the identification and genomic potential of microbes in termite guts, revealing hidden diversity and application potential. However, limitations in culture-based approaches to build on genomic insights have hampered our understanding of the ecology of most of these microbes and the capitalisation on their properties. To help improve culturomics approaches for termite gut microbes, we provide an overview of past and emerging methodologies for isolation and cultivation of symbiotic microbes. In doing so, we highlight future directions and current challenges that need to be overcome to advance these approaches.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"84 ","pages":"Article 108676"},"PeriodicalIF":12.5,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144788177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Algae-driven bioelectrochemical systems: Recent advances, applications, and prospects 藻类驱动的生物电化学系统:最新进展、应用和前景
IF 12.5 1区 工程技术
Biotechnology advances Pub Date : 2025-08-04 DOI: 10.1016/j.biotechadv.2025.108675
Wilgince Apollon , Manisha Verma , Tatiana Kuleshova , Vishal Mishra , Willis Gwenzi , Yamini Mittal , Dipak A. Jadhav , Abudukeremu Kadier , Soumya Ghosh , Alhadji Malloum , Alejandro Isabel Luna-Maldonado , Chikashi Sato , Sathish Kumar Kamaraj
{"title":"Algae-driven bioelectrochemical systems: Recent advances, applications, and prospects","authors":"Wilgince Apollon ,&nbsp;Manisha Verma ,&nbsp;Tatiana Kuleshova ,&nbsp;Vishal Mishra ,&nbsp;Willis Gwenzi ,&nbsp;Yamini Mittal ,&nbsp;Dipak A. Jadhav ,&nbsp;Abudukeremu Kadier ,&nbsp;Soumya Ghosh ,&nbsp;Alhadji Malloum ,&nbsp;Alejandro Isabel Luna-Maldonado ,&nbsp;Chikashi Sato ,&nbsp;Sathish Kumar Kamaraj","doi":"10.1016/j.biotechadv.2025.108675","DOIUrl":"10.1016/j.biotechadv.2025.108675","url":null,"abstract":"<div><div>Bioelectrochemical systems (BESs) are sustainable biotechnologies that have garnered global interest in recent decades. Since their inception, these systems have evolved through various configurations and modifications to enhance performance, prominently featuring microbial fuel cells (MFCs). Researchers are addressing the scaling challenges of MFCs with studies on algae-assisted MFCs (algae-MFCs), which simultaneously generate bioelectricity and treat wastewater cost-effectively. Algae-MFCs are carbon-neutral and photosynthesize to sequester CO<sub>2</sub> while producing oxygen (O<sub>2</sub>) and biomass. O<sub>2</sub> serves as an effective electron acceptor, and biomass is a biofuel feedstock, making the process economical and eco-friendly. This review highlights recent advances in algae-based MFCs, focusing on bioelectricity generation (up to 26,680 mW/m<sup>2</sup>) and biofuel outputs (200 mL/L/h of biohydrogen, 286 mL/g/VS of biomethane, 3.37 g/L of biobutanol, 73 g/L of bioethanol, and 121,104 kg/ha∙year of biodiesel), along with innovations in biokerosene (bio-jet) technology. The impacts of reactor components and configurations on algae-MFC performance, scaling strategies, real-time applications, and computational studies of algae-based BESs are also examined. Furthermore, this review assesses the technoeconomic viability, challenges, and future prospects of this technology. Overall, the findings suggest that algae-MFCs effectively remove contaminants from wastewater and increase power generation while also outlining directions for future advancements.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"84 ","pages":"Article 108675"},"PeriodicalIF":12.5,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144772980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信