Selecting optimal algal strains for robust photosynthetic upgrading of biogas under temperate oceanic climates

Abstract

Biogas generated from anaerobic digestion can be upgraded to biomethane by photosynthetic biogas upgrading, using CO₂ as a bioresource for algal (cyanobacteria and microalgae) cultivation. This allows the upgrading technology to offer economic and environmental benefits to conventional physiochemical upgrading techniques (which can be energy-intensive and costly) by co-generating biomethane with high-value biomass. However, a critical challenge in implementing this technology in temperate oceanic climatic conditions (as found in Japan, and the northwest coasts of Europe and of North America, with average temperatures ranging between 5 and 20 °C) is the selection of algal strains that must be capable of sustained growth under lower ambient temperatures. Accordingly, this paper investigated the selection of algae that met seven key criteria: optimal growth at high pH (9–11); at alkalinity of 1.5–2.5 g inorganic carbon per litre; operation at low temperature (5–20 °C); tolerance to high CO₂ concentrations (above 20 %); capability for mixotrophic cultivation; ability to accumulate high-value metabolites such as photosynthetic pigments and bioactive fatty acids; and ease of harvesting. Of the twenty-six algal species assessed and ranked using a Pugh Matrix, Anabaena sp. and Phormidium sp. were assessed as the most favourable species, followed by Oscillatoria sp., Spirulina subsalsa, and Leptolyngbya sp. Adaptive laboratory evolution together with manipulation of abiotic factors could be effectively utilised to increase the efficiency and economic feasibility of the use of the selected strain in a photosynthetic biogas upgrading system, through improvement of growth and yield of high-value compounds.