酶促CN键形成和裂解反应：进展与展望。

IF 12.5 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology advances Pub Date : 2025-07-22 DOI:10.1016/j.biotechadv.2025.108661

Yaoyun Wu , Wei Song , Wanqing Wei , Jian Wen , Liming Liu , Jing Wu

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引用次数: 0

摘要

辅酶因子依赖酶系统（cnase）在催化CN键的形成和裂解中起着关键作用，CN键是产生多种化学物质的关键反应。本文综述了atp依赖性cnase、烟酰胺依赖性氧化还原酶、黄嘌呤二核苷酸依赖性氧化还原酶、吡哆醛5′-磷酸依赖性cnase、4-甲基咪唑-5- 1依赖性cnase、自由基性cnase、金属性cnase和辅酶非依赖性cnase等关键酶群的分类、结构特征、反应机理以及蛋白质工程对cnase性能的影响。综述了CN键形成和裂解的辅因子依赖性酶系统的最新进展，重点介绍了其关键应用。未来的观点也讨论了关于这些酶系统在建立高效的生物精炼厂的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Enzymatic CN bond formation and cleavage reaction: Advances and perspectives

Cofactor-(in)dependent enzymatic systems (CNases) play a pivotal role in catalyzing the formation and cleavage of CN bonds, a critical reaction for producing a wide range of chemicals. This review provides a comprehensive analysis of the classification, structural characteristics, reaction mechanisms, and the impact of protein engineering on the performance of key enzyme groups, including ATP-dependent CNases, nicotinamide-dependent oxidoreductases, flavin adenine dinucleotide-dependent oxidoreductases, pyridoxal 5′-phosphate dependent CNases, 4-methylideneimidazole-5-one-dependent CNases, radical CNases, metallo-CNases and cofactor-independent CNases. The latest advancements in cofactor-dependent enzymatic systems for CN bond formation and cleavage are summarized, with a focus on key applications. Future perspectives are also discussed regarding the potential of these enzymatic systems in establishing efficient biorefineries.

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来源期刊

Biotechnology advances 工程技术-生物工程与应用微生物

CiteScore

25.50

自引率

2.50%

发文量

167

审稿时长

37 days

期刊介绍： Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.