Biotechnology advances最新文献_第6页

Functional roles and engineering strategies to improve the industrial functionalities of lactic acid bacteria during food fermentation 在食品发酵过程中改善乳酸菌工业功能的功能作用和工程策略。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-06-21 DOI: 10.1016/j.biotechadv.2024.108397

Huan Yang , Liying Hao , Yao Jin , Jun Huang , Rongqing Zhou , Chongde Wu

{"title":"Functional roles and engineering strategies to improve the industrial functionalities of lactic acid bacteria during food fermentation","authors":"Huan Yang , Liying Hao , Yao Jin , Jun Huang , Rongqing Zhou , Chongde Wu","doi":"10.1016/j.biotechadv.2024.108397","DOIUrl":"10.1016/j.biotechadv.2024.108397","url":null,"abstract":"<div><p>In order to improve the flavor profiles, food security, probiotic effects and shorten the fermentation period of traditional fermented foods, lactic acid bacteria (LAB) were often considered as the ideal candidate to participate in the fermentation process. In general, LAB strains possessed the ability to develop flavor compounds via carbohydrate metabolism, protein hydrolysis and amino acid metabolism, lipid hydrolysis and fatty acid metabolism. Based on the functional properties to inhibit spoilage microbes, foodborne pathogens and fungi, those species could improve the safety properties and prolong the shelf life of fermented products. Meanwhile, influence of LAB on texture and functionality of fermented food were also involved in this review. As for the adverse effect carried by environmental challenges during fermentation process, engineering strategies based on exogenous addition, cross protection, and metabolic engineering to improve the robustness and of LAB were also discussed in this review. Besides, this review also summarized the potential strategies including microbial co-culture and metabolic engineering for improvement of fermentation performance in LAB strains. The authors hope this review could contribute to provide an understanding and insight into improving the industrial functionalities of LAB.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":null,"pages":null},"PeriodicalIF":12.1,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cordyceps militaris: A novel mushroom platform for metabolic engineering 冬虫夏草：用于代谢工程的新型蘑菇平台

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-06-19 DOI: 10.1016/j.biotechadv.2024.108396

Jiapeng Zeng , Yue Zhou , Mengdi Lyu , Xinchang Huang , Muyun Xie , Mingtao Huang , Bai-Xiong Chen , Tao Wei

{"title":"Cordyceps militaris: A novel mushroom platform for metabolic engineering","authors":"Jiapeng Zeng , Yue Zhou , Mengdi Lyu , Xinchang Huang , Muyun Xie , Mingtao Huang , Bai-Xiong Chen , Tao Wei","doi":"10.1016/j.biotechadv.2024.108396","DOIUrl":"https://doi.org/10.1016/j.biotechadv.2024.108396","url":null,"abstract":"<div><p><em>Cordyceps militaris</em>, widely recognized as a medicinal and edible mushroom in East Asia, contains a variety of bioactive compounds, including cordycepin (COR), pentostatin (PTN) and other high-value compounds. This review explores the potential of developing <em>C. militaris</em> as a cell factory for the production of high-value chemicals and nutrients. This review comprehensively summarizes the fermentation advantages, metabolic networks, expression elements, and genome editing tools specific to <em>C. militaris</em> and discusses the challenges and barriers to further research on <em>C. militaris</em> across various fields, including computational biology, existing DNA elements, and genome editing approaches. This review aims to describe specific and promising opportunities for the in-depth study and development of <em>C. militaris</em> as a new chassis cell. Additionally, to increase the practicability of this review, examples of the construction of cell factories are provided, and promising strategies for synthetic biology development are illustrated.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":null,"pages":null},"PeriodicalIF":12.1,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0734975024000909/pdfft?md5=bdc99791c7ea807eb642b82e4f245805&pid=1-s2.0-S0734975024000909-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141434589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and applications of lipid hydrophilic headgroups for nucleic acid therapy 用于核酸治疗的脂质亲水头基的开发和应用。

IF 12.1 1区工程技术

Biotechnology advances Pub Date : 2024-06-19 DOI: 10.1016/j.biotechadv.2024.108395

Wanting Ma , Xingxing Fu , Tianyi Zhao , Yanfei Qi , Shubiao Zhang , Yinan Zhao

{"title":"Development and applications of lipid hydrophilic headgroups for nucleic acid therapy","authors":"Wanting Ma , Xingxing Fu , Tianyi Zhao , Yanfei Qi , Shubiao Zhang , Yinan Zhao","doi":"10.1016/j.biotechadv.2024.108395","DOIUrl":"10.1016/j.biotechadv.2024.108395","url":null,"abstract":"<div><p>Nucleic acid therapy is currently the most promising method for treating tumors and genetic diseases and for preventing infectious diseases. However, the biggest obstacle to this therapy is delivery of the nucleic acids to the target site, which requires overcoming problems such as capture by the immune system, the need to penetrate biofilms, and degradation of nucleic acid performance. Designing suitable delivery vectors is key to solving these problems. Lipids—which consist of a hydrophilic headgroup, a linker, and a hydrophobic tail—are crucial components for the construction of vectors. The headgroup is particularly important because it affects the drug encapsulation rate, the vector cytotoxicity, and the transfection efficiency. Herein, we focus on various headgroup structures (tertiary amines, quaternary ammonium salts, peptides, piperazines, dendrimers, and several others), and we summarize and classify important lipid-based carriers that have been developed in recent years. We also discuss applications of cationic lipids with various headgroups for delivery of nucleic acid drugs, and we analyze how headgroup structure affects transport efficiency and carrier toxicity. Finally, we briefly describe the challenges of developing novel lipid carriers, as well as their prospects.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":null,"pages":null},"PeriodicalIF":12.1,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141436625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme-mediated green synthesis of glycosaminoglycans and catalytic process intensification 酶介导的糖胺聚糖绿色合成及催化过程强化。

IF 16 1区工程技术

Biotechnology advances Pub Date : 2024-06-08 DOI: 10.1016/j.biotechadv.2024.108394

Jie Zheng , Xiao-jun Lin , Han Xu , Muhammad Sohail , Liang-an Chen , Xing Zhang

{"title":"Enzyme-mediated green synthesis of glycosaminoglycans and catalytic process intensification","authors":"Jie Zheng , Xiao-jun Lin , Han Xu , Muhammad Sohail , Liang-an Chen , Xing Zhang","doi":"10.1016/j.biotechadv.2024.108394","DOIUrl":"10.1016/j.biotechadv.2024.108394","url":null,"abstract":"<div><p>Glycosaminoglycans (GAGs) are a family of structurally complex heteropolysaccharides that play pivotal roles in biological functions, including the regulation of cell proliferation, enzyme inhibition, and activation of growth factor receptors. Therefore, the synthesis of GAGs is a hot research topic in drug development. The enzymatic synthesis of GAGs has received widespread attention due to their eco-friendly nature, high regioselectivity, and stereoselectivity. The enhancement of the enzymatic synthesis process is the key to its industrial applications. In this review, we overviewed the construction of more efficient <em>in vitro</em> biomimetic synthesis systems of glycosaminoglycans and presented the different strategies to improve enzyme catalysis, including the combination of chemical and enzymatic methods, solid-phase synthesis, and protein engineering to solve the problems of enzyme stability, separation and purification of the product, preparation of structurally defined sugar chains, <em>etc.</em>, and discussed the challenges and opportunities in large-scale green synthesis of GAGs.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":null,"pages":null},"PeriodicalIF":16.0,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141299954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances and opportunities in process analytical technologies for viral vector manufacturing 病毒载体生产过程分析技术的进步与机遇。

IF 16 1区工程技术

Biotechnology advances Pub Date : 2024-06-05 DOI: 10.1016/j.biotechadv.2024.108391

Sobhana A. Sripada , Mahshid Hosseini , Srivatsan Ramesh , Junhyeong Wang , Kimberly Ritola , Stefano Menegatti , Michael A. Daniele

{"title":"Advances and opportunities in process analytical technologies for viral vector manufacturing","authors":"Sobhana A. Sripada , Mahshid Hosseini , Srivatsan Ramesh , Junhyeong Wang , Kimberly Ritola , Stefano Menegatti , Michael A. Daniele","doi":"10.1016/j.biotechadv.2024.108391","DOIUrl":"10.1016/j.biotechadv.2024.108391","url":null,"abstract":"<div><p>Viral vectors are an emerging, exciting class of biologics whose application in vaccines, oncology, and gene therapy has grown exponentially in recent years. Following first regulatory approval, this class of therapeutics has been vigorously pursued to treat monogenic disorders including orphan diseases, entering hundreds of new products into pipelines. Viral vector manufacturing supporting clinical efforts has spurred the introduction of a broad swath of analytical techniques dedicated to assessing the diverse and evolving panel of Critical Quality Attributes (CQAs) of these products. Herein, we provide an overview of the current state of analytics enabling measurement of CQAs such as capsid and vector identities, product titer, transduction efficiency, impurity clearance etc. We highlight orthogonal methods and discuss the advantages and limitations of these techniques while evaluating their adaptation as process analytical technologies. Finally, we identify gaps and propose opportunities in enabling existing technologies for real-time monitoring from hardware, software, and data analysis viewpoints for technology development within viral vector biomanufacturing.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":null,"pages":null},"PeriodicalIF":16.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141287685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nucleic acid-responsive smart systems for controlled cargo delivery 用于控制货物运输的核酸响应智能系统。

IF 16 1区工程技术

Biotechnology advances Pub Date : 2024-05-31 DOI: 10.1016/j.biotechadv.2024.108393

Akbar Hasanzadeh , Arefeh Ebadati , Sara Saeedi , Babak Kamali , Hamid Noori , Behnam Jamei , Michael R. Hamblin , Yong Liu , Mahdi Karimi

引用次数: 0

Toward biomanufacturing of next-generation bacterial nanocellulose (BNC)-based materials with tailored properties: A review on genetic engineering approaches 实现具有定制特性的下一代细菌纳米纤维素（BNC）材料的生物制造：基因工程方法综述。

IF 16 1区工程技术

Biotechnology advances Pub Date : 2024-05-31 DOI: 10.1016/j.biotechadv.2024.108390

Dariela Núñez , Patricio Oyarzún , Sebastián González , Irene Martínez

{"title":"Toward biomanufacturing of next-generation bacterial nanocellulose (BNC)-based materials with tailored properties: A review on genetic engineering approaches","authors":"Dariela Núñez , Patricio Oyarzún , Sebastián González , Irene Martínez","doi":"10.1016/j.biotechadv.2024.108390","DOIUrl":"10.1016/j.biotechadv.2024.108390","url":null,"abstract":"<div><p>Bacterial nanocellulose (BNC) is a biopolymer that is drawing significant attention for a wide range of applications thanks to its unique structure and excellent properties, such as high purity, mechanical strength, high water holding capacity and biocompatibility. Nevertheless, the biomanufacturing of BNC is hindered due to its low yield, the instability of microbial strains and cost limitations that prevent it from being mass-produced on a large scale. Various approaches have been developed to address these problems by genetically modifying strains and to produce BNC-based biomaterials with added value. These works are summarized and discussed in the present article, which include the overexpression and knockout of genes related and not related with the nanocellulose biosynthetic operon, the application of synthetic biology approaches and CRISPR/Cas techniques to modulate BNC biosynthesis. Further discussion is provided on functionalized BNC-based biomaterials with tailored properties that are incorporated <em>in-vivo</em> during its biosynthesis using genetically modified strains either in single or co-culture systems (<em>in-vivo</em> manufacturing). This novel strategy holds potential to open the road toward cost-effective production processes and to find novel applications in a variety of technology and industrial fields.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":null,"pages":null},"PeriodicalIF":16.0,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141186173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The bioproduction of astaxanthin: A comprehensive review on the microbial synthesis and downstream extraction 虾青素的生物生产：关于微生物合成和下游提取的全面综述。

IF 16 1区工程技术

Biotechnology advances Pub Date : 2024-05-31 DOI: 10.1016/j.biotechadv.2024.108392

Dawei Zhou , Zhengyue Fei , Guannan Liu , Yujia Jiang , Wankui Jiang , Carol Sze Ki Lin , Wenming Zhang , Fengxue Xin , Min Jiang

{"title":"The bioproduction of astaxanthin: A comprehensive review on the microbial synthesis and downstream extraction","authors":"Dawei Zhou , Zhengyue Fei , Guannan Liu , Yujia Jiang , Wankui Jiang , Carol Sze Ki Lin , Wenming Zhang , Fengxue Xin , Min Jiang","doi":"10.1016/j.biotechadv.2024.108392","DOIUrl":"10.1016/j.biotechadv.2024.108392","url":null,"abstract":"<div><p>Astaxanthin is a valuable orange-red carotenoid with wide applications in agriculture, food, cosmetics, pharmaceuticals and nutraceuticals areas. At present, the biological synthesis of astaxanthin mainly relies on <em>Haematococcus pluvialis</em> and <em>Xanthophyllomyces dendrorhous</em>. With the rapid development of synthetic biology, more recombinant microbial hosts have been genetically constructed for astaxanthin production including <em>Escherichia coli</em>, <em>Saccharomyces cerevisiae</em> and <em>Yarrowia lipolytica</em>. As multiple genes (15) were involved in the astaxanthin synthesis, it is particularly important to adopt different strategies to balance the metabolic flow towards the astaxanthin synthesis. Furthermore, astaxanthin is a fat-soluble compound stored intracellularly, hence efficient extraction methods are also essential for the economical production of astaxanthin. Several efficient and green extraction methods of astaxanthin have been reported in recent years, including the superfluid extraction, ionic liquid extraction and microwave-assisted extraction. Accordingly, this review will comprehensively introduce the advances on the astaxanthin production and extraction by using different microbial hosts and strategies to improve the astaxanthin synthesis and extraction efficiency.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":null,"pages":null},"PeriodicalIF":16.0,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141199116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The type V effectors for CRISPR/Cas-mediated genome engineering in plants 用于 CRISPR/Cas 介导的植物基因组工程的 V 型效应器。

IF 16 1区工程技术

Biotechnology advances Pub Date : 2024-05-25 DOI: 10.1016/j.biotechadv.2024.108382

Ruixiang Zhang , Nan Chai , Taoli Liu , Zhiye Zheng , Qiupeng Lin , Xianrong Xie , Jun Wen , Zi Yang , Yao-Guang Liu , Qinlong Zhu

{"title":"The type V effectors for CRISPR/Cas-mediated genome engineering in plants","authors":"Ruixiang Zhang , Nan Chai , Taoli Liu , Zhiye Zheng , Qiupeng Lin , Xianrong Xie , Jun Wen , Zi Yang , Yao-Guang Liu , Qinlong Zhu","doi":"10.1016/j.biotechadv.2024.108382","DOIUrl":"10.1016/j.biotechadv.2024.108382","url":null,"abstract":"<div><p>A plethora of CRISPR effectors, such as Cas3, Cas9, and Cas12a, are commonly employed as gene editing tools. Among these, Cas12 effectors developed based on Class II type V proteins exhibit distinct characteristics compared to Class II type VI and type II effectors, such as their ability to generate non-allelic DNA double-strand breaks, their compact structures, and the presence of a single RuvC-like nuclease domain. Capitalizing on these advantages, Cas12 family proteins have been increasingly explored and utilized in recent years. However, the characteristics and applications of different subfamilies within the type V protein family have not been systematically summarized. In this review, we focus on the characteristics of type V effector (CRISPR/Cas12) proteins and the current methods used to discover new effector proteins. We also summarize recent modifications based on engineering of type V effectors. In addition, we introduce the applications of type V effectors for gene editing in animals and plants, including the development of base editors, tools for regulating gene expression, methods for gene targeting, and biosensors. We emphasize the prospects for development and application of CRISPR/Cas12 effectors with the goal of better utilizing toolkits based on this protein family for crop improvement and enhanced agricultural production.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":null,"pages":null},"PeriodicalIF":16.0,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141157550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential of Pavlovophyceae species as a source of valuable carotenoids and polyunsaturated fatty acids for human consumption 巴甫洛夫叶藻物种作为供人类食用的珍贵类胡萝卜素和多不饱和脂肪酸来源的潜力。

IF 16 1区工程技术

Biotechnology advances Pub Date : 2024-05-20 DOI: 10.1016/j.biotechadv.2024.108381

Filipe Maciel , Leandro Madureira , Pedro Geada , José António Teixeira , Joana Silva , António Augusto Vicente

{"title":"The potential of Pavlovophyceae species as a source of valuable carotenoids and polyunsaturated fatty acids for human consumption","authors":"Filipe Maciel , Leandro Madureira , Pedro Geada , José António Teixeira , Joana Silva , António Augusto Vicente","doi":"10.1016/j.biotechadv.2024.108381","DOIUrl":"10.1016/j.biotechadv.2024.108381","url":null,"abstract":"<div><p>Microalgae are a group of microorganisms, mostly photoautotrophs with high CO<sub>2</sub> fixation capacity, that have gained increased attention in the last decades due to their ability to produce a wide range of valuable metabolites, such as carotenoids and polyunsaturated fatty acids, for application in food/feed, pharmaceutical, and cosmeceutical industries. Their increasing relevance has highlighted the importance of identifying and culturing new bioactive-rich microalgae species, as well as of a thorough understanding of the growth conditions to optimize the biomass production and master the biochemical composition according to the desired application. Thus, this review intends to describe the main cell processes behind the production of carotenoids and polyunsaturated fatty acids, in order to understand the possible main triggers responsible for the accumulation of those biocompounds. Their economic value and the biological relevance for human consumption are also summarized. In addition, an extensive review of the impact of culture conditions on microalgae growth performance and their biochemical composition is presented, focusing mainly on the studies involving Pavlovophyceae species. A complementary description of the biochemical composition of these microalgae is also presented, highlighting their potential applications as a promising bioresource of compounds for large-scale production and human and animal consumption.</p></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":null,"pages":null},"PeriodicalIF":16.0,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0734975024000752/pdfft?md5=b1511b628897196870b06df8f89dd336&pid=1-s2.0-S0734975024000752-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141079466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0