Biotechnology advances最新文献_第6页

Plasticity and adaptive architecture of roots for enhanced salinity tolerance in crops 提高作物耐盐性的根系可塑性和适应性结构

IF 12.5 1区工程技术

Biotechnology advances Pub Date : 2026-03-01 Epub Date: 2025-12-02 DOI: 10.1016/j.biotechadv.2025.108773

Faheem Tariq , Linmao Zhao , Saddam Hussain , Muhammad Waheed Riaz , Chenglai Wu , Jiwang Zhang , Pinghua Li , Manje Gowda , Sudha K. Nair , Boddupalli M. Prasanna , Xuecai Zhang , Xianglan Wang , Sunil S. Gangurde

{"title":"Plasticity and adaptive architecture of roots for enhanced salinity tolerance in crops","authors":"Faheem Tariq , Linmao Zhao , Saddam Hussain , Muhammad Waheed Riaz , Chenglai Wu , Jiwang Zhang , Pinghua Li , Manje Gowda , Sudha K. Nair , Boddupalli M. Prasanna , Xuecai Zhang , Xianglan Wang , Sunil S. Gangurde","doi":"10.1016/j.biotechadv.2025.108773","DOIUrl":"10.1016/j.biotechadv.2025.108773","url":null,"abstract":"<div><div>Soil salinization poses a major challenge to global food security, affecting over one billion hectares of arable land and severely constraining crop productivity. As the primary interface between plants and soil, roots play a pivotal role in sensing and adapting to salinity stress through remarkable structural and functional plasticity. This review integrates recent advances in root system architecture (RSA) dynamics, suberin biosynthesis, hormonal regulation, and microbiome interactions to elucidate how plants achieve salinity resilience. We discuss key genes and regulatory modules controlling primary root elongation, lateral root patterning, and barrier formation, emphasizing transcriptional networks involving <em>MYB</em>, <em>NAC</em>, and <em>WRKY</em> families and their coordination with ABA, auxin, and ethylene signaling. Special attention is given to the biosynthesis and deposition of suberin as a dynamic ion-selective barrier governed by hormonal crosstalk and lipid metabolism. We further highlight how beneficial microbes such as <em>Azospirillum</em>, <em>Bacillus</em>, and arbuscular mycorrhizal fungi enhance salt tolerance by modulating phytohormones, antioxidant systems, and ionic homeostasis. Integrating multi-omics and CRISPR-based tools with microbiome engineering offers new avenues to design salt-resilient root ideotypes. We propose a conceptual framework linking molecular regulation, hormonal dynamics, and rhizosphere ecology to root system plasticity, providing a blueprint for engineering next-generation crops capable of maintaining growth and productivity in saline environments.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"87 ","pages":"Article 108773"},"PeriodicalIF":12.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145657155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering strategies for microbial synthesis, customized modification, and application of hemoglobin 血红蛋白的微生物合成、定制修饰和应用的工程策略

IF 12.5 1区工程技术

Biotechnology advances Pub Date : 2026-03-01 Epub Date: 2025-11-14 DOI: 10.1016/j.biotechadv.2025.108752

Fan Liu , Chunxiang Feng , Zirui Yin , Jingwen Zhou , Jianghua Li , Jian Chen , Guocheng Du , Xinrui Zhao

{"title":"Engineering strategies for microbial synthesis, customized modification, and application of hemoglobin","authors":"Fan Liu , Chunxiang Feng , Zirui Yin , Jingwen Zhou , Jianghua Li , Jian Chen , Guocheng Du , Xinrui Zhao","doi":"10.1016/j.biotechadv.2025.108752","DOIUrl":"10.1016/j.biotechadv.2025.108752","url":null,"abstract":"<div><div>Hemoglobin is a functional protein with heme as a cofactor, playing a crucial role in transporting oxygen and maintaining nitric oxide metabolic balance. Besides its physiological functions, hemoglobin has broad potential applications in medicine and biotechnology. However, the widespread use of hemoglobin is constrained by limited natural sources, challenges in heterologous synthesis, and functional restrictions that hinder efficient application. In this review, we discuss the key challenges and solutions associated with microbial synthesis of hemoglobin. We systematically elucidate the engineering strategies to improve the stability, autoxidation rate, heme-binding capacity, oxygen transport efficiency, and nitric oxide scavenging rate of hemoglobin, with particular emphasis on the use of artificial intelligence algorithms to customize the function modification of hemoglobin. Also, we provide a comprehensive overview of the various applications of hemoglobin, including artificial oxygen carriers, medical treatments requiring enhanced oxygen supply, synthesis of high-value products, biocatalysis, artificial foods, agriculture, functional substance testing, and bioactive peptide production, with a special focus on the potential of hemoglobin mutants and derivatives in expanding its use across various fields. Finally, we explore the prospects for accelerating the resolution of hemoglobin synthesis and overcoming the application challenges by integrating Pareto-optimal and iterative bioengineering frameworks, deep learning, synthetic biology, and other advanced technologies.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"87 ","pages":"Article 108752"},"PeriodicalIF":12.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145531338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The advances of strategies and technologies in high-throughput microbial culturomics 高通量微生物培养策略与技术进展

IF 12.5 1区工程技术

Biotechnology advances Pub Date : 2026-03-01 Epub Date: 2025-12-20 DOI: 10.1016/j.biotechadv.2025.108783

Zheng-Hui Li , Shuang Li , Hai-Bo Chen , Yi-Zhi Ji , Chong Zhang

{"title":"The advances of strategies and technologies in high-throughput microbial culturomics","authors":"Zheng-Hui Li , Shuang Li , Hai-Bo Chen , Yi-Zhi Ji , Chong Zhang","doi":"10.1016/j.biotechadv.2025.108783","DOIUrl":"10.1016/j.biotechadv.2025.108783","url":null,"abstract":"<div><div>The growing demand for novel antibiotics, industrial enzymes, and environmentally sustainable biotechnological solutions is drawing increasing attention to more efficient strategies for microbial resource discovery across biomedical, industrial, and ecological domains. To meet this need, high-throughput microbial culturomics has emerged as a powerful strategy that integrates advanced cultivation platforms, diverse growth conditions, and rapid identification technologies. This review explores two principal paradigms within high-throughput microbial culturomics: the function-driven screening-first strategy and the enrichment-based cultivation-first strategy. Their technical foundations, application scenarios, and inherent limitations are examined in detail, providing a comparative analysis of their respective advantages and challenges. We further emphasize the importance of high-throughput identification methods, which play a crucial role in classifying isolates and revealing their functional potential. Ultimately, the review explores future directions for the development of automated, fully integrated cultivation platforms that integrate large-scale experimentation with data-driven optimization. By offering a structured comparison of culturomics strategies, integration pathways, and key obstacles, this review serves as a methodological reference for advancing microbial isolation, functional screening, and biotechnological innovation.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"87 ","pages":"Article 108783"},"PeriodicalIF":12.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145796178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing single and cascade microbial enzyme systems through site-directed mutagenesis for enhancing mycotoxin detoxification 优化单级和级联微生物酶系统通过定点诱变增强霉菌毒素解毒

IF 12.5 1区工程技术

Biotechnology advances Pub Date : 2026-03-01 Epub Date: 2025-11-10 DOI: 10.1016/j.biotechadv.2025.108751

Tosin Victor Adegoke , Sifan Lu , Ogedegbe Gloria Adegoke , Yufei Wang , Yan Wang

{"title":"Optimizing single and cascade microbial enzyme systems through site-directed mutagenesis for enhancing mycotoxin detoxification","authors":"Tosin Victor Adegoke , Sifan Lu , Ogedegbe Gloria Adegoke , Yufei Wang , Yan Wang","doi":"10.1016/j.biotechadv.2025.108751","DOIUrl":"10.1016/j.biotechadv.2025.108751","url":null,"abstract":"<div><div>Mycotoxins, among the most extensively studied biological toxins, pose significant health risks to humans and animals, causing substantial economic losses in the agricultural sector. Numerous conventional enzymes isolated from microorganisms have been reported to detoxify mycotoxin, but their stability is questionable for detoxifying mycotoxin and the direct industrial production of enzymes. Currently, few commercial enzymes are available for the detoxification of mycotoxins. Enhancing enzyme stability is essential to ensure effective detoxification under feed-appropriate temperature and pH conditions. To overcome this challenge, the amalgamation of numerous fields, such as bioinformatics and protein engineering, is crucial for improving the enzyme for industrial production. Computational tools are crucial for determining the nucleotides of the sequence for modification using site-directed mutagenesis (SDM) for the existing conventional enzyme. The SDM technique offers a promising approach for modifying conventional enzymes for commercial purposes. Therefore, focusing on identifying, modifying, and producing enzymes that effectively detoxify mycotoxins is crucial for mitigating their effects on animals and preventing economic losses. Also, a fusion of modified enzymes involved in the cascade detoxification of mycotoxin and its derivatives should be focused on. This review provides an overview of the computational tools and protein engineering approaches, focusing on SDM and cascade catalysis for enhanced mycotoxin detoxification. We also discuss the future directions for incorporating these engineered enzyme systems on a commercial scale.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"87 ","pages":"Article 108751"},"PeriodicalIF":12.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145485498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Building an expanded bio-based economy through synthetic biology 通过合成生物学建立一个扩展的生物经济

IF 12.5 1区工程技术

Biotechnology advances Pub Date : 2026-03-01 Epub Date: 2025-12-06 DOI: 10.1016/j.biotechadv.2025.108775

Andrea M. Garza Elizondo , Ilenne del Valle Kessra , Erica Teixeira Prates , Evan Komp , Elise K. Phillips , Nandhini Ashok , Daniel A. Jacobson , Erin G. Webb , Yannick J. Bomble , William G. Alexander , Joanna Tannous , Chung-Jui Tsai , Wayne A. Parrott , Xiaohan Yang , Breeanna R. Urbanowicz , Laura E. Bartley , Costas D. Maranas , Gerald A. Tuskan , Adam M. Guss , Carrie A. Eckert

{"title":"Building an expanded bio-based economy through synthetic biology","authors":"Andrea M. Garza Elizondo , Ilenne del Valle Kessra , Erica Teixeira Prates , Evan Komp , Elise K. Phillips , Nandhini Ashok , Daniel A. Jacobson , Erin G. Webb , Yannick J. Bomble , William G. Alexander , Joanna Tannous , Chung-Jui Tsai , Wayne A. Parrott , Xiaohan Yang , Breeanna R. Urbanowicz , Laura E. Bartley , Costas D. Maranas , Gerald A. Tuskan , Adam M. Guss , Carrie A. Eckert","doi":"10.1016/j.biotechadv.2025.108775","DOIUrl":"10.1016/j.biotechadv.2025.108775","url":null,"abstract":"<div><div>The field of synthetic biology is essential to the continued development of a bio-based economy, creating mechanisms to supply carbon needed in the economy by both converting existing end-of-life wastes as well as by creating novel, purpose-grown and sustainable feedstocks. Here, we first discuss the near- and long-term resources available for use as feedstocks for bioconversion as well as the output molecules needed for building the foundation of an expanded bio-based economy. We then outline the organisms and phenotypic traits that are needed for the performance-advantaged chassis organisms of the future. Furthermore, we detail the advances, challenges, and opportunities in both microbial and plant synthetic biology relevant to expanding the bio-based economy. Finally, we explore technologies that have and will further enable advances in synthetic biology and the greater bio-based economy.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"87 ","pages":"Article 108775"},"PeriodicalIF":12.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145689942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Droplet microfluidics for single-cell analysis: From improved cell encapsulation and sorting technologies to innovative applications 用于单细胞分析的微滴微流体：从改进的细胞封装和分选技术到创新应用

IF 12.5 1区工程技术

Biotechnology advances Pub Date : 2026-03-01 Epub Date: 2025-12-09 DOI: 10.1016/j.biotechadv.2025.108777

Shuman Song , Jiaran Lu , Xiangyun Jiang , Jiaqi Zhao , Xinran Xiang , Yuting Shang

{"title":"Droplet microfluidics for single-cell analysis: From improved cell encapsulation and sorting technologies to innovative applications","authors":"Shuman Song , Jiaran Lu , Xiangyun Jiang , Jiaqi Zhao , Xinran Xiang , Yuting Shang","doi":"10.1016/j.biotechadv.2025.108777","DOIUrl":"10.1016/j.biotechadv.2025.108777","url":null,"abstract":"<div><div>Single-cell analysis affords a novel perspective for deciphering the intricacies of complex biological systems by elucidating the subtle heterogeneities within cellular populations, with particular relevance to cellular development, pathophysiological mechanisms, and therapeutic responsiveness. At present, numerous studies have reported the application of droplet microfluidics in facilitating high-throughput experimentation at the single-cell level through the precise manipulation of individual cells. However, few comprehensive and systematic reviews have focused on the optimization of droplet microfluidic manipulation strategies and the innovative applications of this technology across various fields. This review discusses the ingenious designs for enhancing cell analysis and highlights their applications in the realms of bioassay, immunotherapy, and drug screening. Furthermore, this review summarizes the current research findings on droplet microfluidics and outlines their future development directions.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"87 ","pages":"Article 108777"},"PeriodicalIF":12.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145731760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosynthesis of high-value chorismate derivatives in Escherichia coli: Recent advances and perspectives 高价值氯酸盐衍生物在大肠杆菌中的生物合成：最新进展和展望

IF 12.5 1区工程技术

Biotechnology advances Pub Date : 2026-03-01 Epub Date: 2025-11-20 DOI: 10.1016/j.biotechadv.2025.108762

Xuewei Pan , Shuran Jiang , Mi Tang , Na Wang , Qisheng Sun , Taowei Yang , Zhiming Rao

{"title":"Biosynthesis of high-value chorismate derivatives in Escherichia coli: Recent advances and perspectives","authors":"Xuewei Pan , Shuran Jiang , Mi Tang , Na Wang , Qisheng Sun , Taowei Yang , Zhiming Rao","doi":"10.1016/j.biotechadv.2025.108762","DOIUrl":"10.1016/j.biotechadv.2025.108762","url":null,"abstract":"<div><div>Chorismate serves not only as a critical branch point for aromatic amino acids biosynthesis, but also as an essential precursor for various high-value aromatic compounds. Currently, the sustainable production of chorismate derivatives is attracting escalating interest due to their pivotal roles as vital components in pharmaceuticals, cosmetics, and specialty chemical intermediates. However, these compounds are commonly obtained through plant extraction or chemical synthesis, which suffer from complex extraction processes, low yields, and environmental burdens, often leading to supply constraints. Therefore, to overcome these challenges, an increasing number of studies are focusing on exploring more production routes for chorismate derivatives to achieve green and efficient synthesis. With the advancement of systems metabolic engineering and contemporary synthetic biology, genetically redesigned <em>Escherichia coli</em> has become pivotal microbial platforms for chorismate derivative biosynthesis. This review outlines the significance of chorismate derivatives and their <em>de novo</em> biosynthetic pathways in <em>E. coli</em>, while reviewing the latest metabolic engineering and synthetic biology strategies employed to boost production of these compounds. Additionally, the persisting challenges and emerging opportunities in leveraging engineered <em>E. coli</em> platforms for industrial-scale biosynthesis of these high-value compounds are discussed.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"87 ","pages":"Article 108762"},"PeriodicalIF":12.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145559757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress in photo-enzyme coupling catalysis for carbon dioxide reduction 光-酶偶联催化二氧化碳还原研究进展

IF 12.5 1区工程技术

Biotechnology advances Pub Date : 2026-03-01 Epub Date: 2025-11-26 DOI: 10.1016/j.biotechadv.2025.108769

Ning Liu, Wenfang Liu

{"title":"Progress in photo-enzyme coupling catalysis for carbon dioxide reduction","authors":"Ning Liu, Wenfang Liu","doi":"10.1016/j.biotechadv.2025.108769","DOIUrl":"10.1016/j.biotechadv.2025.108769","url":null,"abstract":"<div><div>By mimicking natural photosynthesis, the photo-enzyme coupling catalysis (PECC) for carbon dioxide conversion integrates the advantages of photocatalysis and enzymatic catalysis, offering an effective and innovative pathway for capture and utilization of greenhouse gas. This review provides a comprehensive overview of recent advancements in this technology, covering the fundamental principles, key components, synergistic mechanisms, compatibility, and future perspectives. A photo-enzyme coupling system (PECS) can be categorized into cofactor-dependent or cofactor-independent system based on the requirement for cofactor mediation. Its main components include photocatalyst and enzyme, which demonstrates unique advantage in the synergism of energy transfer and substrate activation. In order to improve the compatibility of PECS, the strategies including compartmentalized immobilization and process optimization are employed. By developing highly efficient photocatalyst, strengthening interfacial interaction, and optimizing enzyme engineering, PECC holds great promise for transitioning from laboratory research to industrial application, providing robust support for mitigating global climate change and addressing energy crisis.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"87 ","pages":"Article 108769"},"PeriodicalIF":12.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145609473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review on squalene production by engineered yeasts: Current advances and perspectives 工程酵母生产角鲨烯研究进展及展望。

IF 12.5 1区工程技术

Biotechnology advances Pub Date : 2026-03-01 Epub Date: 2025-12-29 DOI: 10.1016/j.biotechadv.2025.108792

Peng-Cheng Hu , La-Mei Ding , Qiao-Qin Zhao , Mao-Cheng Tang , Pei-Fang Xiao , Chong Wang , Xiang-Yang Lu , Yun Tian , Hu-Hu Liu

{"title":"A review on squalene production by engineered yeasts: Current advances and perspectives","authors":"Peng-Cheng Hu , La-Mei Ding , Qiao-Qin Zhao , Mao-Cheng Tang , Pei-Fang Xiao , Chong Wang , Xiang-Yang Lu , Yun Tian , Hu-Hu Liu","doi":"10.1016/j.biotechadv.2025.108792","DOIUrl":"10.1016/j.biotechadv.2025.108792","url":null,"abstract":"<div><div>Squalene, as a natural triterpenoid exhibiting various physiological activities, is primarily extracted from shark liver oil. However, due to the declining shark populations and conservation concerns, the alternative methods for squalene production are needed. Synthetic biology offers the strategies for engineered yeasts capable of producing squalene. Although the extensive studies have been performed on squalene production by the engineered yeasts, a comprehensive systematic review summarizing these efforts is lack ing. Herein, firstly, this review describes the characteristics of the squalene biosynthesis pathway in yeast cells. Secondly, metabolic strategies for enhancing squalene production in yeasts are summarized. Thirdly, the advanced genetic engineering tools to boost squalene and other terpenoids production are investigated. Fourthly, the potential of emerging other yeasts for squalene synthesis is explored. Finally, the potential technologies applied in yeasts for improving squalene production are discussed. This review will provide comprehensive information on yeasts as chassis for squalene production, laying the foundation for squalene production in yeasts.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"87 ","pages":"Article 108792"},"PeriodicalIF":12.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145877552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthetic biology strategies for engineering probiotics and commensal bacteria for diagnostics and therapeutics 用于诊断和治疗的工程益生菌和共生菌的合成生物学策略

IF 12.5 1区工程技术

Biotechnology advances Pub Date : 2026-03-01 Epub Date: 2025-12-18 DOI: 10.1016/j.biotechadv.2025.108782

Jiwoo Nam , Yuna Lee , Sion Lee , Hyungjun Choi , Sang Yup Lee , Dongsoo Yang

{"title":"Synthetic biology strategies for engineering probiotics and commensal bacteria for diagnostics and therapeutics","authors":"Jiwoo Nam , Yuna Lee , Sion Lee , Hyungjun Choi , Sang Yup Lee , Dongsoo Yang","doi":"10.1016/j.biotechadv.2025.108782","DOIUrl":"10.1016/j.biotechadv.2025.108782","url":null,"abstract":"<div><div>Microorganisms inhabit diverse environments, including nearly every organ in the human body. The human microbiome—a complex community of microorganisms residing in the human body—has gained increasing attention as a key contributor to human health and disease, making it an important target for the development of diagnostic and therapeutic strategies. However, the inherent complexity of microbial communities and the challenges of engineering diverse non-model microorganisms present significant barriers. To address these challenges, synthetic biology has provided powerful tools and strategies to engineer microorganisms capable of sensing disease-specific environments and performing targeted therapeutic functions. In particular, the development of synthetic genetic circuits has significantly improved the precision and reliability of disease diagnosis and treatment, enabling real-time disease monitoring, therapeutic, and even preventive interventions. This review highlights state-of-the-art synthetic biology tools and strategies for engineering the probiotics and commensal bacteria aimed at the diagnosis and treatment of human diseases, with accompanying examples. Future challenges and prospects are also discussed.</div></div>","PeriodicalId":8946,"journal":{"name":"Biotechnology advances","volume":"87 ","pages":"Article 108782"},"PeriodicalIF":12.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145784803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0