Bioscience Reports最新文献_第3页

Retraction: Resveratrol attenuates mechanical compression-induced nucleus pulposus cell apoptosis through regulating the ERK1/2 signaling pathway in a disc organ culture. 撤回：在椎间盘器官培养中，白藜芦醇通过调节ERK1/2信号通路减轻机械压缩诱导的髓核细胞凋亡

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2024-08-28 DOI: 10.1042/BSR-2017-1703_RET

引用次数: 0

Characterization of human aquaporin ion channels in a yeast expression system as a tool for novel ion channel discovery. 在酵母表达系统中表征人类 Aquaporin 离子通道，作为发现新型离子通道的工具。

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2024-08-28 DOI: 10.1042/BSR20240542

Saeed Nourmohammadi, Sam W Henderson, Sunita A Ramesh, Andrea J Yool

{"title":"Characterization of human aquaporin ion channels in a yeast expression system as a tool for novel ion channel discovery.","authors":"Saeed Nourmohammadi, Sam W Henderson, Sunita A Ramesh, Andrea J Yool","doi":"10.1042/BSR20240542","DOIUrl":"10.1042/BSR20240542","url":null,"abstract":"Aquaporin (AQP) channels found in all domains of life are transmembrane proteins which mediate passive transport of water, glycerol, signaling molecules, metabolites, and charged solutes. Discovery of new classes of ion-conducting AQP channels has been slow, likely reflecting time- and labor-intensive methods required for traditional electrophysiology. Work here defines a sensitive mass-throughput system for detecting AQP ion channels, identified by rescue of cell growth in the K+-transport-defective yeast strain CY162 following genetic complementation with heterologously expressed cation-permeable channels, using the well characterized human AQP1 channel for proof of concept. Results showed AQP1 conferred transmembrane permeability to cations which rescued survival in CY162 yeast. Comprehensive testing showed that growth response properties fully recapitulated AQP1 pharmacological agonist and antagonist profiles for activation, inhibition, dose-dependence, and structure-function relationships, demonstrating validity of the yeast screening tool for AQP channel identification and drug discovery efforts. This method also provided new information on divalent cation blockers of AQP1, pH sensitivity of antagonists, and ion permeability of human AQP6. Site-directed mutagenesis of AQP1 channel regulatory domains confirmed that yeast growth rescue was mediated by the introduced channels. Optical monitoring with a lithium-sensitive photoswitchable probe in living cells independently demonstrated monovalent cation permeability of AQP1 channels in yeast plasma membrane. Ion channel properties of AQP1 expressed in yeast were consistent with those of AQP1 expressed in Xenopus laevis oocyte and K+-transport defective Escherichia coli. Outcomes here establish a powerful new approach for efficient screening of phylogenetically diverse AQPs for yet untested functions as cation channels.","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11358751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141787235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction: Mechano growth factor attenuates mechanical overload-induced nucleus pulposus cell apoptosis through inhibiting the p38 MAPK pathway. 撤回：机械生长因子通过抑制 p38 MAPK 通路减轻机械过载诱导的髓核细胞凋亡。

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2024-08-28 DOI: 10.1042/BSR-2018-2462_RET

引用次数: 0

Retraction: Nucleus pulposus cell apoptosis is attenuated by CDMP-2 through regulating oxidative damage under the hyper-osmotic environment. 撤回：CDMP-2通过调节高渗透环境下的氧化损伤来减轻髓核细胞凋亡。

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2024-08-28 DOI: 10.1042/BSR-2018-1176_RET

引用次数: 0

Retraction: Osteogenic protein-1 attenuates nucleus pulposus cell apoptosis through activating the PI3K/Akt/mTOR pathway in a hyperosmotic culture. 撤回：骨生成蛋白-1在高渗培养中通过激活PI3K/Akt/mTOR途径减轻髓核细胞凋亡

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2024-08-28 DOI: 10.1042/BSR-2018-1708_RET

引用次数: 0

Retraction: Role of p38-MAPK pathway in the effects of high-magnitude compression on nucleus pulposus cell senescence in a disc perfusion culture. 撤回：在椎间盘灌注培养中，p38-MAPK通路在高强度挤压对髓核细胞衰老的影响中的作用。

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2024-08-28 DOI: 10.1042/BSR-2017-0718_RET

引用次数: 0

Retraction: The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture. 撤回：椎间盘器官培养中髓核细胞衰老对静态和动态挤压的反应

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2024-08-28 DOI: 10.1042/BSR-2018-0064_RET

引用次数: 0

Transglutaminase 2-mediated histone monoaminylation and its role in cancer. 转谷氨酰胺酶 2 介导的组蛋白单氨酰化及其在癌症中的作用

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2024-08-28 DOI: 10.1042/BSR20240493

Huapeng Li, Jinghua Wu, Nan Zhang, Qingfei Zheng

引用次数: 0

Structural characterization of DNA-binding domain of essential mammalian protein TTF 1. 哺乳动物必需蛋白 TTF 1 DNA 结合域的结构特征。

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2024-08-28 DOI: 10.1042/BSR20240800

Gajender Singh, Abhinetra Jagdish Bhopale, Saloni Khatri, Prashant Prakash, Rajnish Kumar, Sukh Mahendra Singh, Samarendra Kumar Singh

{"title":"Structural characterization of DNA-binding domain of essential mammalian protein TTF 1.","authors":"Gajender Singh, Abhinetra Jagdish Bhopale, Saloni Khatri, Prashant Prakash, Rajnish Kumar, Sukh Mahendra Singh, Samarendra Kumar Singh","doi":"10.1042/BSR20240800","DOIUrl":"10.1042/BSR20240800","url":null,"abstract":"Transcription Termination Factor 1 (TTF1) is a multifunctional mammalian protein with vital roles in various cellular processes, including Pol I-mediated transcription initiation and termination, pre-rRNA processing, chromatin remodelling, DNA damage repair, and polar replication fork arrest. It comprises two distinct functional regions; the N-terminal regulatory region (1-445 aa), and the C-terminal catalytic region (445-859 aa). The Myb domain located at the C-terminal region is a conserved DNA binding domain spanning from 550 to 732 aa (183 residues). Despite its critical role in various cellular processes, the physical structure of TTF1 remains unsolved. Attempts to purify the functional TTF1 protein have been unsuccessful till date. Therefore, we focused on characterizing the Myb domain of this essential protein. We started with predicting a 3-D model of the Myb domain using homology modelling, and ab-initio method. We then determined its stability through MD simulation in an explicit solvent. The model predicted is highly stable, which stabilizes at 200ns. To experimentally validate the computational model, we cloned and expressed the codon optimized Myb domain into a bacterial expression vector and purified the protein to homogeneity. Further, characterization of the protein shows that, Myb domain is predominantly helical (65%) and is alone sufficient to bind the Sal Box DNA. This is the first-ever study to report a complete in silico model of the Myb domain, which is physically characterized. The above study will pave the way towards solving the atomic structure of this essential mammalian protein.","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11358750/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141900877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction: Acidic pH promotes nucleus pulposus cell senescence through activating the p38 MPAK pathway. 撤回：酸性 pH 通过激活 p38 MPAK 通路促进细胞核衰老

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2024-08-28 DOI: 10.1042/BSR-2018-1451_RET

引用次数: 0