Bioscience Reports最新文献_第3页

Retraction: HER2 decreases drug sensitivity of ovarian cancer cells via inducing stem cell-like property in an NFκB-dependent way. 撤回：HER2通过nf κ b依赖性诱导干细胞样特性降低卵巢癌细胞的药物敏感性。

IF 4.7 3区生物学

Bioscience Reports Pub Date : 2025-08-13 DOI: 10.1042/BSR20180829_RET

引用次数: 0

Retraction: The effect of copy number on α-synuclein's toxicity and its protective role in Bax-induced apoptosis, in yeast. 撤回：拷贝数对酵母α-突触核蛋白毒性的影响及其在bax诱导的细胞凋亡中的保护作用。

IF 4.7 3区生物学

Bioscience Reports Pub Date : 2025-08-13 DOI: 10.1042/BSR20201912_RET

引用次数: 0

Unlocking the potential: m6A-RNA methylation in severe epidermolysis bullosa simplex. 释放潜力：严重单纯大疱性表皮松解症中的m6A-RNA甲基化。

IF 4.7 3区生物学

Bioscience Reports Pub Date : 2025-07-22 DOI: 10.1042/BSR20253141

Dario Leonardo Balacco, Benjamin J Hewitt, Ajoy Bardhan, Lisa M Shriane, Manrup Hunjan, Robyn Hickerson, Adrian H M Heagerty, Iain L Chapple

{"title":"Unlocking the potential: m6A-RNA methylation in severe epidermolysis bullosa simplex.","authors":"Dario Leonardo Balacco, Benjamin J Hewitt, Ajoy Bardhan, Lisa M Shriane, Manrup Hunjan, Robyn Hickerson, Adrian H M Heagerty, Iain L Chapple","doi":"10.1042/BSR20253141","DOIUrl":"10.1042/BSR20253141","url":null,"abstract":"Epidermolysis bullosa simplex (EBS) is a rare genetic disorder, resulting from mutations in keratin 5 and keratin 14 (KRT14), and is characterised by skin fragility, herpetiform blistering, and the development of confluent palmoplantar keratoderma and nail dystrophy. Inflammation, pain and itch are the most common complications of severe EBS. However, pathophysiological mechanisms remain poorly characterised at a molecular level. Recently, RNA N6-methyladenosine (m6A) nucleotide modification has been implicated in several cutaneous physiological processes, including epidermal differentiation, inflammation, adaptive immune responses, host-pathogen interactions, wound healing and tissue repair. Nevertheless, the role of m6A in EBS has yet to be defined. In this pilot study, we investigated the gene expression of key regulators of m6A, such as writers Methyltransferase-like 3 and 4 (METTL3 and METTL14), readers YTH domain-containing proteins (YTHDC1, YTHDC2, YTHDC3) and YTH domain-containing family proteins ( YTHDF1 and YTHDF2) and erasers fat mass and obesity-associated (FTO) and alkB homolog 5 (ALKBH5), as well as total RNA m6A levels in the EB keratinocites cell line (KEB-7) derived from a patient with severe EBS, carrying the KRT14 R125P mutation. NEB-1 cells, derived from a healthy donor, were employed as controls. RNAseq and quantitative RT-PCR demonstrated up-regulation of the writer METTL14, while FTO was down-regulated. Moreover, the total RNA m6A colorimetric assay reported higher levels of m6A in severe EBS cells (KEB-7). Additionally, increased expression of the reader of YTHDC1 suggests a dysregulation of downstream pathways. These findings suggest a potential role for m6A in determining complications in severe EBS; however, its role and effects need to be fully elucidated.","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":"45 7","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411834/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction: NANOG regulates epithelial mesenchymal transition and chemoresistance in ovarian cancer. 缩回：NANOG调节卵巢癌上皮间质转化和化疗耐药。

IF 4.7 3区生物学

Bioscience Reports Pub Date : 2025-07-17 DOI: 10.1042/BSR20160247_RET

引用次数: 0

Development and validation of direct assay for cholesterol content of erythrocytes. 红细胞胆固醇含量直接测定法的建立与验证。

IF 4.7 3区生物学

Bioscience Reports Pub Date : 2025-07-17 DOI: 10.1042/BSR20253335

Azusa Yamazaki, Yuna Hakii, Akira Yoshimoto, Takahiro Kameda, Naoya Ichimura, Shuji Tohda, Ryunosuke Ohkawa

{"title":"Development and validation of direct assay for cholesterol content of erythrocytes.","authors":"Azusa Yamazaki, Yuna Hakii, Akira Yoshimoto, Takahiro Kameda, Naoya Ichimura, Shuji Tohda, Ryunosuke Ohkawa","doi":"10.1042/BSR20253335","DOIUrl":"10.1042/BSR20253335","url":null,"abstract":"Erythrocytes contain a significant amount of membrane cholesterol, which is continuously exchanged with lipoproteins. Recent studies suggest that erythrocyte cholesterol content correlates positively with atherosclerotic cardiovascular disease (ASCVD) severity independent of low-density lipoprotein levels, potentially reflecting residual ASCVD risk. However, conventional methods for measuring erythrocyte cholesterol content require labor-intensive lipid extraction procedures, limiting their clinical applicability. In this study, we developed a novel enzymatic assay that enables direct quantification of erythrocyte total cholesterol content using two denaturants to eliminate hemoglobin interference. This simple method demonstrated high accuracy and precision, as confirmed by intra-assay repeatability, between-day precision, linearity, and spike-recovery tests. Using this assay, we determined the erythrocyte cholesterol content per volume (154.8 ± 2.9 mg/dl in men, 155.9 ± 6.9 mg/dl in women) and per cell (139.0 ± 5.2 fg/cell in men, 140.8 ± 5.3 fg/cell in women) (n = 12, healthy subjects). While erythrocyte cholesterol content per volume correlated with the conventional method, the erythrocyte cholesterol content per cell showed no such correlation. Moreover, neither measure was associated with serum lipid levels, suggesting their potential as independent biomarkers for ASCVD. Additionally, we evaluated erythrocyte cholesterol content across different maturation stages and found that older erythrocytes had significantly lower cholesterol content, consistent with mass spectrometry results. These findings further validated the physiological relevance of the proposed method. In conclusion, we successfully established a simple and clinically applicable enzymatic method for measuring erythrocyte cholesterol content, providing novel insights into erythrocyte cholesterol metabolism and its potential role in ASCVD risk assessment.","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crystal structures and low-affinity complex formation of halogenase CtcP and FAD reductase CtcQ from the chlortetracycline biosynthetic pathway. 氯四环素生物合成途径中卤素酶CtcP和FAD还原酶CtcQ的晶体结构和低亲和力复合物的形成。

IF 4.7 3区生物学

Bioscience Reports Pub Date : 2025-07-04 DOI: 10.1042/BSR20253185

Caixia Hou, Sylvie Garneau-Tsodikova, Oleg V Tsodikov

{"title":"Crystal structures and low-affinity complex formation of halogenase CtcP and FAD reductase CtcQ from the chlortetracycline biosynthetic pathway.","authors":"Caixia Hou, Sylvie Garneau-Tsodikova, Oleg V Tsodikov","doi":"10.1042/BSR20253185","DOIUrl":"10.1042/BSR20253185","url":null,"abstract":"Enzymatic halogenation in natural products has been intensely investigated due to its potential utility as a tool to improve pharmacological and pharmaceutical properties of drug leads. Chlortetracycline (CTC), the first tetracycline (TC) antibiotic discovered nearly eight decades ago, contains a chlorine group. This chlorine is installed enzymatically by the flavin adenine dinucleotide (FAD)-dependent halogenase CtcP. CtcP and the FAD reductase CtcQ, which is also encoded in the CTC biosynthetic gene cluster, function as a two-component system. Structural information on CtcP and CtcQ has been lacking. In this study, we determined crystal structures of CtcP from Kitasatospora aureofaciens in a complex with polyethylene glycol and sulfate ions and in a complex with FAD, and a crystal structure of CtcQ in a complex with FAD and NAD. The structures of CtcP revealed a close similarity of this enzyme to the phenolic halogenase PltM, despite a large difference in the sizes of their respective substrates, presumably TC and phloroglucinol. The CtcP structure showed a conserved dimeric organization also found in PltM crystals. We showed that dimerization of CtcP is allosterically influenced by a distant C-terminal helical hairpin. A closed substrate-binding cavity of CtcP suggested that conformational changes were required to allow a substrate, likely not TC, to bind CtcP. We demonstrated that CtcP and CtcQ weakly bound each other. The dimeric structures of CtcP and CtcQ prompted us to propose approximate models of a 2:2/CtcP:CtcQ complex, where FAD(H2) would shuttle between the two enzymes for chlorination and reduction.","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12400496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction: Up-regulation of long non-coding RNA SNHG20 promotes ovarian cancer progression via Wnt/β-catenin signaling. 撤回：长链非编码RNA SNHG20上调通过Wnt/β-catenin信号传导促进卵巢癌进展。

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2025-06-23 DOI: 10.1042/BSR20170681_RET

引用次数: 0

Modulating adipose-derived stromal cells' secretomes by culture conditions: effects on angiogenesis, collagen deposition, and immunomodulation. 通过培养条件调节脂肪源性基质细胞的分泌：对血管生成、胶原沉积和免疫调节的影响。

IF 4.7 3区生物学

Bioscience Reports Pub Date : 2025-05-30 DOI: 10.1042/BSR20241389

Erika Pinheiro-Machado, Claudia C Koster, Alexandra M Smink

{"title":"Modulating adipose-derived stromal cells' secretomes by culture conditions: effects on angiogenesis, collagen deposition, and immunomodulation.","authors":"Erika Pinheiro-Machado, Claudia C Koster, Alexandra M Smink","doi":"10.1042/BSR20241389","DOIUrl":"10.1042/BSR20241389","url":null,"abstract":"The secretome of adipose-derived stromal cells (ASCs) presents a promising avenue for cell-free therapies due to its rich mixture of bioactive molecules. Different culture conditions can modulate the composition of this mixture, but how this affects the functional properties of the secretome remains to be investigated. This study investigated the in vitro effects of normoxia, cytokines, high glucose, hypoxia, and hypoxia + high glucose-derived ASC secretomes on angiogenesis (tube formation assay), collagen deposition (Picrosirius red staining), and immunomodulation (one-way mixed lymphocyte reaction in combination with an antibody-mediated cell-dependent cytotoxicity assay). The data showed that normoxia- and hypoxiaderived secretomes consistently exhibited potent proangiogenic effects in both human and rat models. These secretomes also demonstrated positive influences on collagen deposition and immunomodulation. Interestingly, the human ASC hypoxia + high glucose-derived secretome emerged as a stimulator of collagen deposition and modulator of the immune system. Conversely, cytokines and high glucose-derived secretomes have shown less strong effects in almost all functional parameters. In conclusion, our findings indicate that modulating culturing conditions results in secretomes with different functional properties and emphasizes the multifaceted role of ASC secretomes in regenerative processes.","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":" ","pages":"325-342"},"PeriodicalIF":4.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential role of polydatin in treating diabetes mellitus and diabetes-related chronic complications. 多聚糖在治疗糖尿病及糖尿病相关慢性并发症中的潜在作用。

IF 4.7 3区生物学

Bioscience Reports Pub Date : 2025-05-30 DOI: 10.1042/BSR20241307

Farjana Yasmin, Kim San Tang

{"title":"Potential role of polydatin in treating diabetes mellitus and diabetes-related chronic complications.","authors":"Farjana Yasmin, Kim San Tang","doi":"10.1042/BSR20241307","DOIUrl":"10.1042/BSR20241307","url":null,"abstract":"Diabetes mellitus is a complex metabolic disorder associated with severe complications affecting various organs, including the kidneys, nerves, heart, and blood vessels. Managing these complications remains a significant clinical challenge, necessitating the exploration of novel therapeutic approaches. This review focuses on polydatin, a naturally occurring glycoside from Polygonum cuspidatum, highlighting its potential as a multitargeted therapeutic agent against diabetic complications. Evidence indicates that polydatin effectively improves insulin sensitivity, lowers blood glucose levels, and exhibits antioxidant properties. In diabetic nephropathy, polydatin has been shown to reduce oxidative stress, inflammation, and podocyte apoptosis, thereby preserving renal function. Furthermore, it enhances mitochondrial function and Sirt1 expression in diabetic neuropathy, promoting nerve regeneration and alleviating pain. In cardiac studies, polydatin protects against diabetic cardiomyopathy by enhancing autophagy and reducing oxidative stress, ultimately improving cardiac function. Additionally, polydatin restores endothelial function in vascular complications associated with diabetes. Polydatin presents a promising natural therapy with the potential to mitigate multiple complications of diabetes through its antioxidant, anti-inflammatory, and cytoprotective effects. Although findings from animal models and in vitro studies are promising, further clinical research is essential to validate its efficacy and safety in human subjects. By integrating polydatin into diabetes management strategies, there is potential for improved health outcomes and quality of life for individuals affected by this chronic condition.","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":"45 5","pages":"303-323"},"PeriodicalIF":4.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell RNAseq of Angiotensin II-induced abdominal aortic tissue identifies aneurysm-associated cell clusters in C57BL/6J mice. 血管紧张素ii诱导的腹主动脉组织单细胞RNAseq鉴定C57BL/6J小鼠动脉瘤相关细胞簇。

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2025-05-28 DOI: 10.1042/BSR20241235

Huimin Li, Xueyu Hao, Peng Zhang, Jun Guo, Wei Li

{"title":"Single-cell RNAseq of Angiotensin II-induced abdominal aortic tissue identifies aneurysm-associated cell clusters in C57BL/6J mice.","authors":"Huimin Li, Xueyu Hao, Peng Zhang, Jun Guo, Wei Li","doi":"10.1042/BSR20241235","DOIUrl":"10.1042/BSR20241235","url":null,"abstract":"Abdominal aortic aneurysms (AAAs) are life-threatening due to the rupture of aorta. Different vascular cell types are known to be involved in AAA development. However, whether any specific cell cluster plays a critical role during AAA formation is unknown. Angiotensin II (Ang II) infused mouse AAA models are commonly used to study the development and progression of AAA. We here investigate the incidence of AAA at different ages or different doses of Ang II in C57BL/6J mice. There was no AAA formation at a concentration of 1.44 mg/kg/day or 2.16 mg/kg/day at the age of 14 weeks. At the age of 20 weeks and 32 weeks, the incidence of AAA was 18.2% (6/21) and 57.1% (4/7), respectively, with a concentration of 1.44 mg/kg/day. Using single-cell RNA sequencing, we found that increased clusters of monocytes and neutrophils, macrophages, T cells, and B cells were the typical changes in AAA. A special cluster transformed from endothelial cells (malignant ECs) was identified, in which genesinvolved in lipid metabolism, including Cd36, Lpl, Gpihbp1, Fabp4, and Pparg, were highly expressed. Mice receiving Ang II treatment without AAA development showed increased fibroblasts, which may prevent the occurrence of AAA. Through cell-cell interaction analysis, we found that the Cxcl12-Cxcr4/Ackr3 axis, which functions in inflammatory ligand- receptor binding, may play a role in AAA formation. Our results reveal that specific cell clusters may contribute to the progression or prevention of AAA formation. These findings provide new clues for the pathogenesis and intervention of AAA.","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":"45 5","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0