Bioscience Reports最新文献

Unlocking the potential: m6A-RNA methylation in severe epidermolysis bullosa simplex. 释放潜力：严重单纯大疱性表皮松解症中的m6A-RNA甲基化。

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2025-07-22 DOI: 10.1042/BSR20253141

Dario Leonardo Balacco, Benjamin J Hewitt, Ajoy Bardhan, Lisa M Shriane, Manrup Hunjan, Robyn Hickerson, Adrian H M Heagerty, Iain L Chapple

{"title":"Unlocking the potential: m6A-RNA methylation in severe epidermolysis bullosa simplex.","authors":"Dario Leonardo Balacco, Benjamin J Hewitt, Ajoy Bardhan, Lisa M Shriane, Manrup Hunjan, Robyn Hickerson, Adrian H M Heagerty, Iain L Chapple","doi":"10.1042/BSR20253141","DOIUrl":"https://doi.org/10.1042/BSR20253141","url":null,"abstract":"Epidermolysis bullosa simplex (EBS) is a rare genetic disorder, resulting from mutations in keratin 5 and keratin 14 (KRT14), and is characterised by skin fragility, herpetiform blistering, and the development of confluent palmoplantar keratoderma and nail dystrophy. Inflammation, pain and itch are the most common complications of severe EBS. However, pathophysiological mechanisms remain poorly characterised at a molecular level. Recently, RNA N6-methyladenosine (m6A) nucleotide modification has been implicated in several cutaneous physiological processes, including epidermal differentiation, inflammation, adaptive immune responses, host-pathogen interactions, wound healing and tissue repair. Nevertheless, the role of m6A in EBS has yet to be defined. In this pilot study, we investigated the gene expression of key regulators of m6A, such as writers Methyltransferase-like 3 and 4 (METTL3 and METTL14), readers YTH domain-containing proteins (YTHDC1, YTHDC2, YTHDC3) and YTH domain-containing family proteins ( YTHDF1 and YTHDF2) and erasers fat mass and obesity-associated (FTO) and alkB homolog 5 (ALKBH5), as well as total RNA m6A levels in the EB keratinocites cell line (KEB-7) derived from a patient with severe EBS, carrying the KRT14 R125P mutation. NEB-1 cells, derived from a healthy donor, were employed as controls. RNAseq and quantitative RT-PCR demonstrated up-regulation of the writer METTL14, while FTO was down-regulated. Moreover, the total RNA m6A colorimetric assay reported higher levels of m6A in severe EBS cells (KEB-7). Additionally, increased expression of the reader of YTHDC1 suggests a dysregulation of downstream pathways. These findings suggest a potential role for m6A in determining complications in severe EBS; however, its role and effects need to be fully elucidated.","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":"45 7","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144697472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction: NANOG regulates epithelial mesenchymal transition and chemoresistance in ovarian cancer. 缩回：NANOG调节卵巢癌上皮间质转化和化疗耐药。

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2025-07-17 DOI: 10.1042/BSR20160247_RET

引用次数: 0

Development and validation of direct assay for cholesterol content of erythrocytes. 红细胞胆固醇含量直接测定法的建立与验证。

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2025-07-17 DOI: 10.1042/BSR20253335

Azusa Yamazaki, Yuna Hakii, Akira Yoshimoto, Takahiro Kameda, Naoya Ichimura, Shuji Tohda, Ryunosuke Ohkawa

{"title":"Development and validation of direct assay for cholesterol content of erythrocytes.","authors":"Azusa Yamazaki, Yuna Hakii, Akira Yoshimoto, Takahiro Kameda, Naoya Ichimura, Shuji Tohda, Ryunosuke Ohkawa","doi":"10.1042/BSR20253335","DOIUrl":"10.1042/BSR20253335","url":null,"abstract":"Erythrocytes contain a significant amount of membrane cholesterol, which is continuously exchanged with lipoproteins. Recent studies suggest that erythrocyte cholesterol content correlates positively with atherosclerotic cardiovascular disease (ASCVD) severity independent of low-density lipoprotein levels, potentially reflecting residual ASCVD risk. However, conventional methods for measuring erythrocyte cholesterol content require labor-intensive lipid extraction procedures, limiting their clinical applicability. In this study, we developed a novel enzymatic assay that enables direct quantification of erythrocyte total cholesterol content using two denaturants to eliminate hemoglobin interference. This simple method demonstrated high accuracy and precision, as confirmed by intra-assay repeatability, between-day precision, linearity, and spike-recovery tests. Using this assay, we determined the erythrocyte cholesterol content per volume (154.8 ± 2.9 mg/dl in men, 155.9 ± 6.9 mg/dl in women) and per cell (139.0 ± 5.2 fg/cell in men, 140.8 ± 5.3 fg/cell in women) (n = 12, healthy subjects). While erythrocyte cholesterol content per volume correlated with the conventional method, the erythrocyte cholesterol content per cell showed no such correlation. Moreover, neither measure was associated with serum lipid levels, suggesting their potential as independent biomarkers for ASCVD. Additionally, we evaluated erythrocyte cholesterol content across different maturation stages and found that older erythrocytes had significantly lower cholesterol content, consistent with mass spectrometry results. These findings further validated the physiological relevance of the proposed method. In conclusion, we successfully established a simple and clinically applicable enzymatic method for measuring erythrocyte cholesterol content, providing novel insights into erythrocyte cholesterol metabolism and its potential role in ASCVD risk assessment.","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crystal structures and low-affinity complex formation of halogenase CtcP and FAD reductase CtcQ from the chlortetracycline biosynthetic pathway. 氯四环素生物合成途径中卤素酶CtcP和FAD还原酶CtcQ的晶体结构和低亲和力复合物的形成。

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2025-07-04 DOI: 10.1042/BSR20253185

Caixia Hou, Sylvie Garneau-Tsodikova, Oleg V Tsodikov

{"title":"Crystal structures and low-affinity complex formation of halogenase CtcP and FAD reductase CtcQ from the chlortetracycline biosynthetic pathway.","authors":"Caixia Hou, Sylvie Garneau-Tsodikova, Oleg V Tsodikov","doi":"10.1042/BSR20253185","DOIUrl":"10.1042/BSR20253185","url":null,"abstract":"Enzymatic halogenation in natural products has been intensely investigated due to its potential utility as a tool to improve pharmacological and pharmaceutical properties of drug leads. Chlortetracycline (CTC), the first tetracycline (TC) antibiotic discovered nearly eight decades ago, contains a chlorine group. This chlorine is installed enzymatically by the flavin adenine dinucleotide (FAD)-dependent halogenase CtcP. CtcP and the FAD reductase CtcQ, which is also encoded in the CTC biosynthetic gene cluster, function as a two-component system. Structural information on CtcP and CtcQ has been lacking. In this study, we determined crystal structures of CtcP from Kitasatospora aureofaciens in a complex with polyethylene glycol and sulfate ions and in a complex with FAD, and a crystal structure of CtcQ in a complex with FAD and NAD. The structures of CtcP revealed a close similarity of this enzyme to the phenolic halogenase PltM, despite a large difference in the sizes of their respective substrates, presumably TC and phloroglucinol. The CtcP structure showed a conserved dimeric organization also found in PltM crystals. We showed that dimerization of CtcP is allosterically influenced by a distant C-terminal helical hairpin. A closed substrate-binding cavity of CtcP suggested that conformational changes were required to allow a substrate, likely not TC, to bind CtcP. We demonstrated that CtcP and CtcQ weakly bound each other. The dimeric structures of CtcP and CtcQ prompted us to propose approximate models of a 2:2/CtcP:CtcQ complex, where FAD(H2) would shuttle between the two enzymes for chlorination and reduction.","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction: Up-regulation of long non-coding RNA SNHG20 promotes ovarian cancer progression via Wnt/β-catenin signaling. 撤回：长链非编码RNA SNHG20上调通过Wnt/β-catenin信号传导促进卵巢癌进展。

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2025-06-23 DOI: 10.1042/BSR20170681_RET

引用次数: 0

Single-cell RNAseq of Angiotensin II-induced abdominal aortic tissue identifies aneurysm-associated cell clusters in C57BL/6J mice. 血管紧张素ii诱导的腹主动脉组织单细胞RNAseq鉴定C57BL/6J小鼠动脉瘤相关细胞簇。

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2025-05-28 DOI: 10.1042/BSR20241235

Huimin Li, Xueyu Hao, Peng Zhang, Jun Guo, Wei Li

{"title":"Single-cell RNAseq of Angiotensin II-induced abdominal aortic tissue identifies aneurysm-associated cell clusters in C57BL/6J mice.","authors":"Huimin Li, Xueyu Hao, Peng Zhang, Jun Guo, Wei Li","doi":"10.1042/BSR20241235","DOIUrl":"10.1042/BSR20241235","url":null,"abstract":"Abdominal aortic aneurysms (AAAs) are life-threatening due to the rupture of aorta. Different vascular cell types are known to be involved in AAA development. However, whether any specific cell cluster plays a critical role during AAA formation is unknown. Angiotensin II (Ang II) infused mouse AAA models are commonly used to study the development and progression of AAA. We here investigate the incidence of AAA at different ages or different doses of Ang II in C57BL/6J mice. There was no AAA formation at a concentration of 1.44 mg/kg/day or 2.16 mg/kg/day at the age of 14 weeks. At the age of 20 weeks and 32 weeks, the incidence of AAA was 18.2% (6/21) and 57.1% (4/7), respectively, with a concentration of 1.44 mg/kg/day. Using single-cell RNA sequencing, we found that increased clusters of monocytes and neutrophils, macrophages, T cells, and B cells were the typical changes in AAA. A special cluster transformed from endothelial cells (malignant ECs) was identified, in which genesinvolved in lipid metabolism, including Cd36, Lpl, Gpihbp1, Fabp4, and Pparg, were highly expressed. Mice receiving Ang II treatment without AAA development showed increased fibroblasts, which may prevent the occurrence of AAA. Through cell-cell interaction analysis, we found that the Cxcl12-Cxcr4/Ackr3 axis, which functions in inflammatory ligand- receptor binding, may play a role in AAA formation. Our results reveal that specific cell clusters may contribute to the progression or prevention of AAA formation. These findings provide new clues for the pathogenesis and intervention of AAA.","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":"45 5","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of protein or glutamine biosynthesis affect the light-induced dephosphorylation of the SBiP1 chaperone in Symbiodiniaceae. 抑制蛋白质或谷氨酰胺的生物合成会影响共生菌科中SBiP1伴侣蛋白的光诱导去磷酸化。

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2025-05-20 DOI: 10.1042/BSR20241085

Raúl Eduardo Castillo-Medina, Tania Islas-Flores, Estefanía Morales-Ruiz, Marco Villanueva

{"title":"Inhibition of protein or glutamine biosynthesis affect the light-induced dephosphorylation of the SBiP1 chaperone in Symbiodiniaceae.","authors":"Raúl Eduardo Castillo-Medina, Tania Islas-Flores, Estefanía Morales-Ruiz, Marco Villanueva","doi":"10.1042/BSR20241085","DOIUrl":"10.1042/BSR20241085","url":null,"abstract":"Phosphorylation/dephosphorylation is fundamental for transduction of external stimuli into physiological responses. In photosynthetic dinoflagellates Symbiodinium microadriaticum CassKB8, Thr-phosphorylated SBiP1 under dark conditions, undergoes significant dephosphorylation upon light stimuli. We evaluated the effect of protein synthesis inhibitors on light modulated Thr phosphorylation of SBiP1. Inhibition of cytoplasmic protein synthesis by cycloheximide but not of chloroplastic protein synthesis by chloramphenicol, prevented Thr dephosphorylation of the protein under the light. Additionally, inhibition of glutamine synthetase by glufosinate produced a delay in the light induced dephosphorylation of the chaperone. Heat shock reverted the effect in cycloheximide-treated cells suggesting that heat stress overrides the cycloheximide-induced inhibition of SBiP1 dephosphorylation to hypothetically restore chaperone activity. These results suggest that light and stress are critical switches of SBiP1 chaperone activity that function along with common pathways of protein synthesis and ammonia assimilation, and further confirm that the light induced SBiP1 Thr dephosphorylation is independent of photosynthesis.","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The PAD4 inhibitor GSK484 diminishes neutrophil extracellular trap in the colon mucosa but fails to improve inflammatory biomarkers in experimental colitis. PAD4抑制剂GSK484可以减少结肠粘膜中的中性粒细胞胞外陷阱，但不能改善实验性结肠炎的炎症生物标志物。

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2025-05-20 DOI: 10.1042/BSR20253205

Kangzhe Xie, Jordan Hunter, Aaron Lee, Gulfam Ahmad, Paul K Witting, Tamara Ortiz-Cerda

{"title":"The PAD4 inhibitor GSK484 diminishes neutrophil extracellular trap in the colon mucosa but fails to improve inflammatory biomarkers in experimental colitis.","authors":"Kangzhe Xie, Jordan Hunter, Aaron Lee, Gulfam Ahmad, Paul K Witting, Tamara Ortiz-Cerda","doi":"10.1042/BSR20253205","DOIUrl":"10.1042/BSR20253205","url":null,"abstract":"Inflammatory bowel disease (IBD) is a gastrointestinal disorder characterised by elevated colonic neutrophil extracellular traps (NETs), which are associated with disease severity. Formation of NETs is primarily driven by peptidyl arginine deaminase IV (PAD4) and other enzymes including myeloperoxidase (MPO) and neutrophil elastase. The present study evaluated the effect of MPO and PAD4 inhibition in dextran sodium sulfate (DSS)-induced colitis. Experimental colitis was induced in male C57BL/6 mice by 2% w/v DSS in drinking water ad libitum. Treatment groups received daily oral administration of MPO inhibitor (AZD3241; 30 mg/kg) and/or intraperitoneal injection of PAD4 inhibitor (GSK484; 4 mg/kg) 4 times over 9 Inhibition of PAD4 significantly diminished NET density in the colonic mucosa of mice insulted with DSS, reaching levels similar to that detected in control mice. Both inhibitors offered limited improvement in disease-activity-index, a scoring system that considers the extent of weight loss, stool consistency and rectal bleeding. Histology showed that MPO and/or PAD4 inhibition did not recover DSS-induced colon histoarchitectural damage whilst Alcian blue staining demonstrated that PAD4 failed to reduce goblet cell loss. The selected dosage of PAD4 inhibition also yielded no effect on inflammatory markers and antioxidant protein levels. These data sets suggest that other mechanisms may be involved in the pathogenesis of IBD, and the appropriate dosage of GSK484 requires thorough investigation.","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12236107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of a surfactin-like biosurfactant produced by Bacillus subtilis 3KP isolated from oil-contaminated soil in Indonesia. 印尼石油污染土壤中枯草芽孢杆菌3KP类表面活性剂的分离与表征

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2025-04-29 DOI: 10.1042/BSR20241227

Hanif Yuliani, Nimatuzahroh Nimatuzahroh, Handoko Darmokoesoemo, Hery Suwito, Eko Agus Nugroho, Moch Affandi, Agus Supriyanto, Fatimah Fatimah, Silvia Kurnia Sari, Ana Mariatul Khiftiyah

{"title":"Isolation and characterization of a surfactin-like biosurfactant produced by Bacillus subtilis 3KP isolated from oil-contaminated soil in Indonesia.","authors":"Hanif Yuliani, Nimatuzahroh Nimatuzahroh, Handoko Darmokoesoemo, Hery Suwito, Eko Agus Nugroho, Moch Affandi, Agus Supriyanto, Fatimah Fatimah, Silvia Kurnia Sari, Ana Mariatul Khiftiyah","doi":"10.1042/BSR20241227","DOIUrl":"https://doi.org/10.1042/BSR20241227","url":null,"abstract":"Biosurfactants are surface-active compounds that have many benefits. This research aims to isolate and identify biosurfactants produced by B. subtilis 3KP when cultured on a molasses substrate. The isolation and purification procedure included gradual ammonium sulfate precipitation, ion exchange and adsorption chromatography. Identification and characterization using high-pressure liquid chromatography, gas chromatography-mass spectrometry, amino acid analyzer, UV-V is spectroscopy, and infrared spectroscopy confirmed that B. subtilis 3KP biosurfactant is a lipopeptide. The biosurfactant produced by B. subtilis 3KP was determined to be a surfactin isoform, characterized by a 16-carbon fatty acid chain. The purified biosurfactant successfully decreased the surface tension from 72 mN/m to 27 mN/m and reached the critical micelle concentration at a precise value of 20.01 mg/L. This biosurfactant product is stable at 30°C-65°C, pH 4-8, and salinity 0-4 M. Therefore, the presented isolate has tremendous potential to produce surfactin-type biosurfactants which can be developed and utilized for various industrial fields.","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulating adipose-derived stromal cells' secretomes by culture conditions: effects on angiogenesis, collagen deposition, and immunomodulation. 通过培养条件调节脂肪源性基质细胞的分泌：对血管生成、胶原沉积和免疫调节的影响。

IF 3.8 3区生物学

Bioscience Reports Pub Date : 2025-04-23 DOI: 10.1042/BSR20241389

Erika Pinheiro-Machado, Claudia Koster, Alexandra Smink

{"title":"Modulating adipose-derived stromal cells' secretomes by culture conditions: effects on angiogenesis, collagen deposition, and immunomodulation.","authors":"Erika Pinheiro-Machado, Claudia Koster, Alexandra Smink","doi":"10.1042/BSR20241389","DOIUrl":"10.1042/BSR20241389","url":null,"abstract":"The secretome of adipose-derived stromal cells (ASC) presents a promising avenue for cell-free therapies due to their rich mixture of bioactive molecules. Different culture conditions can modulate the composition of this mixture, but how this affects the functional properties of the secretome remains to be investigated. This study investigated the in vitro effects of normoxia, cytokines, high glucose, hypoxia, and hypoxia + high glucose-derived ASC secretomes on angiogenesis (tube formation assay), collagen deposition (Picrosirius-Red staining), and immunomodulation (One-way Mixed Lymphocyte Reaction in combination with an antibody-mediated cell-dependent cytotoxicity assay). The data showed that normoxia and hypoxia-derived secretomes consistently exhibited potent proangiogenic effects in both human and rat models. These secretomes also demonstrated positive influences on collagen deposition and immunomodulation. Interestingly, the human ASC hypoxia + high glucose-derived secretome emerged as a stimulator of collagen deposition and modulator of the immune system. Conversely, cytokines and high glucose-derived secretomes have shown less strong effects in almost all functional parameters. In conclusion, our findings indicate that modulating culturing conditions results in secretomes with different functional properties and emphasizes the multifaceted role of ASC secretomes in regenerative processes.","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0