Bioscience Reports最新文献

{"title":"Toward safer and sustainable food preservation: a comprehensive review of bacteriocins in the food industry.","authors":"José Carlos Parada Fabián, Ana Karen Álvarez Contreras, Iván Natividad Bonifacio, Marcos Francisco Hernández Robles, Carlos Ramón Vázquez Quiñones, Elsa Irma Quiñones Ramírez, Carlos Vázquez Salinas","doi":"10.1042/BSR20241594","DOIUrl":"10.1042/BSR20241594","url":null,"abstract":"<p><p>Bacteriocins are considered promising natural biopreservatives in the food industry because of their broad spectrum of antimicrobial activity against Gram-positive bacteria and foodborne pathogens. This review provides information on several bacteriocins (nisin, pediocin, Micocin®, lacticin 3147, and enterocin AS-48), their mechanisms of action, applications, and discussion of regulatory requirements for their approval as food additives by the Food and Drug Administration (FDA) and the European Union to improve food safety. Nisin (the most studied bacteriocin), recognized as generally regarded as safe by the FDA, is used as a food preservative. Pediocin, derived from Pediococcus acidilactici, shows efficacy against Listeria species and is used in vegetable and meat products. Micocin®, a mixture of bacteriocins produced by Carnobacterium maltaromaticum CB1, is effective against Clostridium botulinum and Listeria monocytogenes. Lacticin 3147, composed of two peptides: Ltnα and Ltnβ, shows synergistic antibacterial activity with potential applications in the control of pathogens in dairy products. Enterococcin AS-48, produced by Enterococcus faecalis subsp. liquefaciens S-48, exhibits broad-spectrum antimicrobial activity against several Gram-positive bacteria and has been studied for biopreservation in a number of food products. For regulatory approval, the following criteria must be met: determination of identity, chemical composition, safety assessments, and recommended concentrations for use. Despite the difficulties posed by their large-scale production and purification, bacteriocins hold enormous potential for improving food safety and shelf life; however, further research is required to harness bacteriocins as future food preservation strategies.</p>","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":"45 4","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The final proteolytic step in transmembrane signaling of multiple RsgI anti-σ factors in Clostridium thermocellum.","authors":"Wen Wen, Chao Chen, Qiu Cui, Jinsong Xuan, Yingang Feng","doi":"10.1042/BSR20253055","DOIUrl":"10.1042/BSR20253055","url":null,"abstract":"<p><p>In Clostridium thermocellum, there are nine RsgI factors responsible for sensing different types of substrates and regulating the transcription and expression of cellulosome genes. Within the signaling pathway of RsgI, the membrane protease RseP cleaves RsgI in its transmembrane helix, thus releasing the N-terminal fragment of RsgI from the membrane. This released RsgI N-terminal fragment is subsequently recognized and degraded by a cytoplasmic protease complex consisting of an AAA+ ATPase and ClpP protease. Previous research showed that the ClpXP complex, comprising ClpX and ClpP, is capable of recognizing and degrading the N-terminal fragment of RsgI6. However, due to the low conservation of the transmembrane helical region of RsgI, it remains unclear whether other RsgIs are similarly recognized and degraded by the same unfoldase. In this study, we employed in vitro protease assays to examine the recognition and degradation of the N-terminal fragment of each RsgI by various ClpP-unfoldase complexes. Results confirm that ClpXP is responsible for degrading the N-terminal fragments of all RsgI proteins in C. thermocellum, suggesting a degree of sequence promiscuity in substrate recognition by ClpXP. ClpXP can recognize multiple XAA sites in the transmembrane helix region of RsgI. Moreover, we unexpectedly discovered that the cytoplasmic domain influences the degradation of RsgI2-NF by ClpXP in our in vitro assay. This study provides new insights into understanding the complex regulatory mechanisms of cellulosome genes and the role of AAA+ proteases in C. thermocellum, thereby offering critical clues for unraveling the internal regulatory networks of bacteria.</p>","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203970/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143794298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of an epigenetic signature of allostatic load.","authors":"Jonviea D Chamberlain, Daniel Ackermann, Murielle Bochud, Tom Booth, Laurence Chapatte, Janie Corley, Simon R Cox, Sarah E Harris, Cassandre Kinnaer, Robert-Paul Juster, Isabella Locatelli, David Nanchen, Belène Ponte, Menno Pruijm, Sylvain Pradervand, Paul G Shiels, Silvia Stringhini, Sébastien Nusslé, Semira Gonseth-Nusslé","doi":"10.1042/BSR20241663","DOIUrl":"10.1042/BSR20241663","url":null,"abstract":"<p><p>The allostatic load (AL) concept measures physiological dysregulation in response to internal and external stressors that accumulate across the life course. AL has been consistently linked to chronic disease risk across studies. However, there is considerable variation in its operationalization. In the present study, DNA methylation (DNAm) data (using the Illumina Infinium MethylationEPIC BeadChip array) from the Swiss Kidney Project on Genes in Hypertension (SKIPOGH) cohort, a Swiss-based family cohort study, were used in a discovery epigenome-wide association study to identify cytosine-guanine nucleotide sites associated with phenotypic measures of AL. Elastic net linear regression models were used to estimate an epigenetic signature of AL (methAL), including an Illumina HumanMethylation450K (HM450K) assay-compatible signature (methALT). The methALT signature was validated in the 1936 Lothian Birth Cohort (LBC1936), population-based prospective cohort study. We found that the methAL signature was positively associated with the clinical phenotype of AL in both the SKIPOGH (R2 = 0.59) and LBC1936 (R2 = 0.16) cohorts. In the validation cohort, a one standard deviation increase in methALT signature was associated with 25% higher odds of reported history of cardiovascular disease (CVD) (odd ratio [OR] = 1.25, 95% confidence interval [CI] = 1.05-1.50), and a nearly two-fold increase in all-cause mortality rate at the beginning of follow-up (hazard ratio = 1.68, 95% CI = 1.33-2.13) when adjusting for all potential confounders. In conclusion, the epigenetic signature for AL not only correlated well with phenotype-based AL scores but also exhibited a stronger association with the history of CVD and all-cause mortality compared with AL scores. The methAL signature could help assuage issues of comparison across studies.</p>","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and functional validation of ACSL1 as a biomarker regulating ferroptosis in nucleus pulposus cell.","authors":"Yichi Zhou, Ke Wang, Min Ren, Jiebin Wang, Fanglin Wang, Bingbing Zhuang, Lin Chen, Zhiqiang Zhang, Changsheng Wang","doi":"10.1042/BSR20241414","DOIUrl":"10.1042/BSR20241414","url":null,"abstract":"<p><p>Intervertebral disc degeneration (IVDD) is a prevalent musculoskeletal disorder characterized by the deterioration of nucleus pulposus (NP) cells, leading to significant impairments in patients' quality of life. Elucidating the molecular mechanisms underlying IVDD is essential for developing effective therapeutic strategies. In this study, we utilized weighted gene co-expression network analysis to identify key module eigengenes (MEs) from the GSE124272 dataset, combined with differential gene expression analysis to pinpoint differentially expressed genes (DEGs). Functional enrichment analysis revealed that MEs were primarily associated with lipid metabolism and immune response, while DEGs were enriched in immune response and cell proliferation pathways. By integrating MEs, DEGs, and ferroptosis-related genes, we identified six hub genes (acyl-CoA synthetase long-chain family member 1 [ACSL1], BACH1, CBS, CP, AKR1C1, and AKR1C3). Consensus clustering analysis classified samples into two immune-related subgroups, C1 and C2, with single-sample gene set enrichment analysis demonstrating distinct immune scores between the subgroups. Notably, ACSL1 showed the strongest correlation with immune cell infiltration and was significantly up-regulated in the C1 subgroup, which exhibited higher immune scores. In vitro experiments confirmed elevated ACSL1 expression in an IL-1β-induced degenerative NP cell model. Silencing ACSL1 improved cell viability, reduced apoptosis, and restored mitochondrial membrane potential, alongside significant changes in intracellular Fe2+, malondialdehyde, and glutathione levels. In vivo experiments further validated increased ACSL1 expression in intervertebral disc tissues of IVDD rats. Collectively, these findings highlight ACSL1 as a potential biomarker for the early diagnosis of IVDD and a promising therapeutic target.</p>","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143555800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Comparative assessment of the bactericidal effect of nanoparticles of copper oxide, silver, and chitosan-silver against Escherichia coli infection in broilers.","authors":"","doi":"10.1042/BSR20204091_COR","DOIUrl":"10.1042/BSR20204091_COR","url":null,"abstract":"","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":"45 3","pages":"211"},"PeriodicalIF":3.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12225058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: The effect of different concentrations of gold nanoparticles on growth performance, toxicopathological and immunological parameters of broiler chickens.","authors":"","doi":"10.1042/BSR20194296_COR","DOIUrl":"10.1042/BSR20194296_COR","url":null,"abstract":"","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":"45 3","pages":"213"},"PeriodicalIF":3.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12225062/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of UDP-glucose pyrophosphorylases from different organisms.","authors":"Siqi Zhang, Xin Song, Yuqi Qin","doi":"10.1042/BSR20241494","DOIUrl":"https://doi.org/10.1042/BSR20241494","url":null,"abstract":"<p><p>UDP-glucose pyrophosphorylases (UGPases) catalyze the conversion of UTP and glucose-1-phosphate (Glc1P) to UDP-glucose and pyrophosphate, playing crucial roles in cell metabolism. The UGPases are related to the biosynthesis of glycans in various organisms and linked to bacterial survival, plant programmed cell death, and even human cancers. Eleven UGPases from the bacterium Escherichia coli; fungi Saccharomyces cerevisiae (ScUGP) and Aspergillus niger (AnUGP); plants Hordeum vulgare (barley) (HvUGP), Arabidopsis thaliana (AtUGP), Solanum tuberosum (potato) (StUGP), Manihot esculenta (cassava) (MeUGP), Ipomoea batatas (sweet potato) (IbUGP), and Zea mays (maize) (ZmUGP); and animals Drosophila melanogaster (fruit fly) (DmUGP) and Homo sapiens (human) (HsUGP) were expressed in E. coli and assayed. MeUGP and StUGP have the highest and second-highest specific activities, respectively. The second-order rate constant kcat/KM values of 11 UGPases are ranked from high to low in the following order: MeUGP > StUGP > ZmUGP > IbUGP > AtUGP > AnUGP > HvUGP > HsUGP > DmUGP > ScUGP > EcUGP. EcUGP, ScUGP, AnUGP, HvUGP, AtUGP, DmUGP, and HsUGP show a temperature optimum of 37℃. MeUGP, IbUGP, and ZmUGP showed a temperature optimum of 50℃. Overall, recombinant UGPases were not thermally stable. Ten UGPases were rapidly inactivated at 60℃ except for IbUGP. The recombinant UGPases use Glc1P with high activities. UGPases exhibit variations in NTP utilization efficiency. The results improve the knowledge of the characteristics of UGPase from various organisms and provide the potential to use MeUGP or StUGP as the engineering target of cell factories.</p>","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143771242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural analysis of genetic variants of the human tumor suppressor PALB2 coiled-coil domain.","authors":"Pothula Purushotham Reddy, Apurva Phale, Ranabir Das","doi":"10.1042/BSR20241173","DOIUrl":"10.1042/BSR20241173","url":null,"abstract":"<p><p>The tumor suppressor PALB2 is a key player in the homologous recombination (HR) pathway, functionally connecting BRCA proteins at the DNA damage site. PALB2 forms homodimers via its coiled-coil domain, and during HR, it forms a heterodimeric complex with BRCA1 using the same domain. However, the structural details of the human PALB2 coiled-coil domain are unknown. Several missense variants have been reported in the coiled-coil domain. The structure-function relationship of these variants is poorly understood, posing a challenge to genetic counseling. In this study, we present the solution structure of the human PALB2 coiled-coil domain, which forms an antiparallel homodimer. We then use this structure to investigate the impact of a few well-characterized missense mutations on the fold and interactions of the PALB2 coiled-coil domain. Our findings reveal a strong correlation between the structural impact of mutations and their efficiency in homologous recombination, suggesting that our approach can be applied to study other genetic variations in PALB2. These findings hold promise for improving genetic counseling and advancing cancer research.</p>","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interferon-induced ADP-ribosylation: technical developments driving ICAB discovery.","authors":"Victoria Chaves Ribeiro, Lilian Cristina Russo, Dulce María González Duré, Nícolas Carlos Hoch","doi":"10.1042/BSR20240986","DOIUrl":"10.1042/BSR20240986","url":null,"abstract":"<p><p>Cells respond to a variety of internal and external stimuli by regulating the activities of different signalling cascades and cellular processes, often via chemical modifications of biological macromolecules that modulate their overall levels, biochemical activities or biophysical interactions. One such modification, termed ADP-ribosylation (ADPr), is emerging as an important player in the interferon (IFN) response, but the molecular targets and functions of ADP-ribosyltransferases within this core component of innate immunity still remains unclear. We and others have recently identified that stimulation of IFN signalling cascades promotes the formation of a novel cytosolic structure in human cells that is enriched in ADP-ribosyl modifications. Here, we propose to name these structures 'interferon-induced cytosolic ADPr bodies' (ICABs) and discuss their known components and potential functions. We also review methods to detect ICABs (and cellular ADPr in general) using a range of recently developed reagents. This lays the foundation for future studies aimed at elucidating the molecular functions of ICABs and ADPr in innate immune responses, which is a central unanswered question in the field.</p>","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096948/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginger extract promotes pancreatic islets regeneration in streptozotocin-induced diabetic rats.","authors":"Manal S Abbood, Amani M Al-Adsani, Suzanne A Al-Bustan","doi":"10.1042/BSR20241510","DOIUrl":"10.1042/BSR20241510","url":null,"abstract":"<p><p>Ginger (Zingiber officinale) exerts an antidiabetic effect by restoring pancreatic β-cells. The present study aimed to investigate the mechanism by which ginger extract induces the regeneration of functional β-cells in diabetic rats. Sprague-Dawley rats (n=27) were divided into three groups: normal rats given double distilled water (ddH2O) (NC, n=11), diabetic rats (injected with 60 mg/kg streptozotocin) given ddH2O (DC, n=8), and diabetic rats treated with aqueous ginger extract (DG, n=8). The effect of ginger extract intake on the differential expression of neurogenin-3 (Neurog3), V-maf musculoaponeurotic fibrosarcoma oncogene homolog B (Mafb), insulin 2 (Ins2), and glucagon (Gcg) was assessed using quantitative real-time PCR after one and eight weeks of treatment. The pancreatic insulin source was determined using immunohistochemical analysis. After one week, ginger treatment significantly up-regulated the expression of both Neurog3 and Mafb in the DG rats compared with the DC rats. However, after eight weeks, the mRNA levels of these genes dropped significantly in parallel with the up-regulation of Ins2 and Gcg expression, resulting in increased serum insulin levels, weight, and lowered fasting blood glucose levels. Immunohistochemical analysis revealed a restored β-cell mass and islet architecture in the DG group. Ginger extract exerts an antidiabetic effect by acting on pancreatic progenitors and α-cells to restore β-cell mass in streptozotocininduced diabetic rats. These findings suggest that ginger extract could be a potential stimulator of β-cell neogenesis, which provides an alternative to meet the increasing demand for exogenous insulin in patients with diabetes.</p>","PeriodicalId":8926,"journal":{"name":"Bioscience Reports","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12096951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}