通过培养条件调节脂肪源性基质细胞的分泌:对血管生成、胶原沉积和免疫调节的影响。

IF 3.8 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Erika Pinheiro-Machado, Claudia Koster, Alexandra Smink
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引用次数: 0

摘要

脂肪源性基质细胞(ASC)分泌组由于其丰富的生物活性分子混合物而为无细胞治疗提供了一条有前途的途径。不同的培养条件可以调节这种混合物的组成,但这如何影响分泌组的功能特性仍有待研究。本研究探讨了常氧、细胞因子、高糖、低氧和低氧+高糖衍生的ASC分泌组对血管生成(管形成实验)、胶原沉积(Picrosirius-Red染色)和免疫调节(单向混合淋巴细胞反应结合抗体介导的细胞依赖性细胞毒性实验)的体外影响。数据显示,在人类和大鼠模型中,常氧和低氧衍生的分泌组一致表现出强大的促血管生成作用。这些分泌组对胶原沉积和免疫调节也有积极影响。有趣的是,人类ASC缺氧+高糖衍生分泌组作为胶原沉积的刺激因子和免疫系统的调节剂出现。相反,细胞因子和高糖衍生的分泌组在几乎所有功能参数中都显示出较弱的作用。总之,我们的研究结果表明,调节培养条件可以产生具有不同功能特性的分泌体,并强调了ASC分泌体在再生过程中的多方面作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Modulating adipose-derived stromal cells' secretomes by culture conditions: effects on angiogenesis, collagen deposition, and immunomodulation.

The secretome of adipose-derived stromal cells (ASC) presents a promising avenue for cell-free therapies due to their rich mixture of bioactive molecules. Different culture conditions can modulate the composition of this mixture, but how this affects the functional properties of the secretome remains to be investigated. This study investigated the in vitro effects of normoxia, cytokines, high glucose, hypoxia, and hypoxia + high glucose-derived ASC secretomes on angiogenesis (tube formation assay), collagen deposition (Picrosirius-Red staining), and immunomodulation (One-way Mixed Lymphocyte Reaction in combination with an antibody-mediated cell-dependent cytotoxicity assay). The data showed that normoxia and hypoxia-derived secretomes consistently exhibited potent proangiogenic effects in both human and rat models. These secretomes also demonstrated positive influences on collagen deposition and immunomodulation. Interestingly, the human ASC hypoxia + high glucose-derived secretome emerged as a stimulator of collagen deposition and modulator of the immune system. Conversely, cytokines and high glucose-derived secretomes have shown less strong effects in almost all functional parameters. In conclusion, our findings indicate that modulating culturing conditions results in secretomes with different functional properties and emphasizes the multifaceted role of ASC secretomes in regenerative processes.

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来源期刊
Bioscience Reports
Bioscience Reports 生物-细胞生物学
CiteScore
8.50
自引率
0.00%
发文量
380
审稿时长
6-12 weeks
期刊介绍: Bioscience Reports provides a home for sound scientific research in all areas of cell biology and molecular life sciences. Since 2012, Bioscience Reports has been fully Open Access and publishes all papers under the liberal CC BY licence, giving the life science community quality research to share and discuss.Content before 2012 is subscription-only, and is accessible via archive purchase. Articles are assessed on soundness, providing a home for valid findings and data. We welcome papers that span disciplines (e.g. chemistry, medicine), including papers describing: -new methodologies -tools and reagents to probe biological questions -mechanistic details -disease mechanisms -metabolic processes and their regulation -structure and function -bioenergetics
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