Biomedical Chromatography最新文献_第8页

Simultaneous Quantification of Filgotinib and Its Active Metabolite in Human Plasma Using Liquid Chromatography–Tandem Mass Spectrometry: Validation and Clinical Application 液相色谱-串联质谱法同时定量人血浆中非戈替尼及其活性代谢物：验证和临床应用

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-19 DOI: 10.1002/bmc.70030

Takahiro Ito, Manabu Suno, Minae Shintani, Ayaka Iwata, Takao Fujii, Kazuo Matsubara

{"title":"Simultaneous Quantification of Filgotinib and Its Active Metabolite in Human Plasma Using Liquid Chromatography–Tandem Mass Spectrometry: Validation and Clinical Application","authors":"Takahiro Ito, Manabu Suno, Minae Shintani, Ayaka Iwata, Takao Fujii, Kazuo Matsubara","doi":"10.1002/bmc.70030","DOIUrl":"https://doi.org/10.1002/bmc.70030","url":null,"abstract":"<p>Filgotinib (FLG) is a Janus kinase 1 inhibitor and is metabolized to an active metabolite, GS-829845. There is no report on the method for simultaneous quantification of FLG and GS-829845 in clinical samples. We developed a liquid chromatography–tandem mass spectrometry method for simultaneous determination of FLG and GS-829845 in patient plasma. FLG and GS-829845 were extracted from an aliquot of 50 μL of human plasma by simple deproteinization using methanol. Chromatographic separation was performed using a Shim-pack Scepter C18-120 column with a combined mobile phase of water and methanol containing 0.1% formic acid and gradient elution at a flow rate of 0.2 mL/min. Detection was performed by positive electrospray ionization using a QTRAP 4500 mass spectrometer. The method was validated in the concentration range of 2.5–50 ng/mL for FLG and 250–5000 ng/mL for GS-829845. Intra- and inter-day assay accuracy and precision were within 11.4% and 13.9%, respectively. Recoveries and matrix effects were consistent and reproducible. This developed and fully validated method is simple, rapid, and cost-effective and was used successfully for therapeutic drug monitoring in a patient with rheumatoid arthritis.</p>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bmc.70030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of Pectolinarin in Rat Plasma Using UPLC-MS/MS and Its Pharmacokinetic Analysis UPLC-MS/MS定量大鼠血浆中Pectolinarin及其药动学分析

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-19 DOI: 10.1002/bmc.70032

Mengmeng Shao, Runrun Wang, Congcong Wen, Xianqin Wang, Yongxi Jin, Saiya Chen

{"title":"Quantification of Pectolinarin in Rat Plasma Using UPLC-MS/MS and Its Pharmacokinetic Analysis","authors":"Mengmeng Shao, Runrun Wang, Congcong Wen, Xianqin Wang, Yongxi Jin, Saiya Chen","doi":"10.1002/bmc.70032","DOIUrl":"https://doi.org/10.1002/bmc.70032","url":null,"abstract":"<div>\u0000 \u0000 <p>Pectolinarin is a flavonoid compound known for its wound-healing properties, including anti-inflammatory and antibacterial effects. In this study, we employed UPLC-MS/MS to quantify pectolinarin in rat plasma and investigate its pharmacokinetics. Plasma samples were processed using an acetonitrile precipitation method. Chromatographic separation was performed on a UPLC BEH column with a gradient mobile phase of acetonitrile-water (containing 0.1% formic acid). Detection was carried out using electrospray ionization (ESI) tandem mass spectrometry in multiple reaction monitoring (MRM) mode with positive ionization, targeting transitions of <i>m</i>/<i>z</i> 623.3 → 315.3 for pectolinarin and <i>m</i>/<i>z</i> 370.5 → 125.0 for the IS. The results demonstrated that pectolinarin exhibited acceptable linearity in rat plasma within the concentration range of 1.2 to 2300 ng/mL (<i>r</i> > 0.995). The intraday and interday precision, expressed as relative standard deviation (RSD), was below 9.2%. Accuracy ranged from 97.3% to 108.3%, with average recovery exceeding 94.7%. The matrix effect was between 97.8% and 105.3%. The method was successfully applied to evaluate the pharmacokinetics of pectolinarin in rats following both oral and intravenous administration. The absolute bioavailability of pectolinarin in rats was determined to be 0.28%.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolite Profiling of Danatinib in Mice Using Ultra-High-Performance Liquid Chromatography/Tandem Mass Spectrometry In Vitro and In Vivo 用超高效液相色谱/串联质谱法分析丹纳替尼在小鼠体内和体外的代谢产物

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-12 DOI: 10.1002/bmc.70022

Meng-Yuan Zhang, Yuan-Wei Zhang, Sheng-Nan Yu, Nuo Qiao, Xing-Feng Ni, Qian Liu, Ming-Yu Bai, Qing-Qing Li, Zi-Xuan Wang, Yun Zhou, Ning Ding, Shan-Liang Sun, Xin Xue, Chen-Xiao Shan, Zhi-Hao Shi, Jin Wang, Nian-Guang Li

{"title":"Metabolite Profiling of Danatinib in Mice Using Ultra-High-Performance Liquid Chromatography/Tandem Mass Spectrometry In Vitro and In Vivo","authors":"Meng-Yuan Zhang, Yuan-Wei Zhang, Sheng-Nan Yu, Nuo Qiao, Xing-Feng Ni, Qian Liu, Ming-Yu Bai, Qing-Qing Li, Zi-Xuan Wang, Yun Zhou, Ning Ding, Shan-Liang Sun, Xin Xue, Chen-Xiao Shan, Zhi-Hao Shi, Jin Wang, Nian-Guang Li","doi":"10.1002/bmc.70022","DOIUrl":"https://doi.org/10.1002/bmc.70022","url":null,"abstract":"<div>\u0000 \u0000 <p>Danatinib, a brand-new compound synthesized in our laboratory for the treatment of acute myeloid leukemia (AML), demonstrates remarkable antitumor activity. However, the pharmacokinetic profile of Danatinib in mice was short with its half-life was only 0.476 h. To address this issue and optimize its therapeutic efficacy, this study investigated the metabolic profiles of Danatinib in mice, both in vitro and in vivo, using ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF MS) technology. In the in vitro study, Danatinib was analyzed using mouse liver microsomes, resulting in the identification of eight metabolites. In the in vivo study, Danatinib was administered orally to mice at 20 mg/kg; the samples of plasma, bile, feces, and urine were collected and analyzed, leading to the identification of 34 metabolites. The results showed that those metabolites were formed through various metabolic reactions including hydroxylation, carboxylation, acetylation, hydrogenation, and glucuronidation. This study provides a systematic investigation of the metabolism of Danatinib, offering valuable information for further structural modification to improve its pharmacokinetic profiles.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systematic Investigation of Metabolism and Potential Pharmacological Mechanism of Tanreqing Injection to Human Lower Respiratory Tract Infection Based on UPLC–Q–TOF–MSE and Network Pharmacology 基于UPLC-Q-TOF-MSE和网络药理学的痰热清注射液对人下呼吸道感染代谢及潜在药理机制的系统研究

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-12 DOI: 10.1002/bmc.70024

Huanlu Wang, Xi Mai, Yan Jing, Shuhao Liu

{"title":"Systematic Investigation of Metabolism and Potential Pharmacological Mechanism of Tanreqing Injection to Human Lower Respiratory Tract Infection Based on UPLC–Q–TOF–MSE and Network Pharmacology","authors":"Huanlu Wang, Xi Mai, Yan Jing, Shuhao Liu","doi":"10.1002/bmc.70024","DOIUrl":"https://doi.org/10.1002/bmc.70024","url":null,"abstract":"<div>\u0000 \u0000 <p>Tanreqing injection (TRQI), a well-known traditional Chinese medicine, has a marked curative effect on lower respiratory tract infections. A strategy using ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC–Q–TOF–MS<sup>E</sup>) for rapid identification of metabolites from TRQI was proposed by UNIFI informatics platform combined with multiple data processing techniques. Target prediction was then performed based on the original compounds in vivo, and a network between the active compounds and common targets was established using Cytoscape v3.9.0. As a result, 64 original compounds were characterized in TRQI, and 54 original compounds and 76 metabolites of TRQI were detected in human plasma and urine, two of which (M28 and M45) were novel metabolites. A novel metabolic pathway for lonicerin was identified. The compound-target-pathway network identified 22 target genes and 20 signaling pathways that were linked to these mechanisms. The key mechanism is related to the JAK-STAT signaling pathway and PI3K-Akt signaling pathway. The bioactive ingredients and mechanisms of action of TQRI against lower respiratory tract infections based on original compounds in vivo were explored through network pharmacology and molecular docking. This is the first study in which the mechanism of action of TRQI in humans has been clarified.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Blood Absorption Components by UPLC-Q-TOF-MS/MS From Anemarrhenae Rhizoma Before and After Salt Processing UPLC-Q-TOF-MS/MS法分析海参盐处理前后血液吸收成分

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-12 DOI: 10.1002/bmc.70025

Wang Ya-zhu, Wang Xiao-ting, Tian Shu-qing, Gao Hui

引用次数: 0

Bridging the Gap: Eco-Friendly HPLC Analysis of Metformin and Lobeglitazone Sulfate Despite Polarity and Dosage Differences 弥合差距：尽管极性和剂量不同，二甲双胍和硫酸洛贝列酮的生态友好型HPLC分析

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-11 DOI: 10.1002/bmc.70013

Honey Kevat, Ayushi Prajapati, Kiran Parmar, Bhumika Maheriya, Hardi Joshi, Jigna Vadalia, Kashyap Thummar

{"title":"Bridging the Gap: Eco-Friendly HPLC Analysis of Metformin and Lobeglitazone Sulfate Despite Polarity and Dosage Differences","authors":"Honey Kevat, Ayushi Prajapati, Kiran Parmar, Bhumika Maheriya, Hardi Joshi, Jigna Vadalia, Kashyap Thummar","doi":"10.1002/bmc.70013","DOIUrl":"https://doi.org/10.1002/bmc.70013","url":null,"abstract":"<div>\u0000 \u0000 <p>A precise and stability-indicating high-performance liquid chromatographic (HPLC) method was developed and validated for the simultaneous estimation of lobeglitazone sulfate (0.5 mg) and metformin hydrochloride (500 mg) in pharmaceutical formulations. This method effectively addresses the analytical challenge posed by the significantly different dosage strengths and contrasting polarities of lobeglitazone (water insoluble) and metformin (water soluble). Utilizing a Phenomenex Strong Cation Exchange (250 mm × 4.6 mm, 10 μm) column, the method employed a mobile phase of ammonium dihydrogen phosphate (pH 3.0) and a premix of methanol and acetonitrile (80:20), delivered at a flow rate of 1.0 mL/min. The PDA detector was set at 251 nm to monitor eluents, and the column temperature was maintained at 30°C for optimal separation. The method was validated as per ICH guidelines, covering specificity, linearity, accuracy, precision, LOD, LOQ, solution stability, and robustness. Forced degradation studies under various stress conditions (acidic, basic, oxidative, photolytic, and thermal) revealed no significant degradation products. The method was also assessed for greenness using a AGREE tool, achieving a score of 0.68, demonstrating its eco-friendly nature. Overall, this method is suitable for the routine analysis of these drugs in pharmaceutical formulations.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Separation and Quantitative Estimation of Pharmacopeial Organic Impurities, Process-Related Impurities, and Degradation Products of the Anti-Schizophrenia Drug Clozapine in Pharmaceutical Formulations by Using Stability-Indicating HPLC Method 稳定性指示高效液相色谱法分离和定量评价抗精神分裂症药物氯氮平制剂中药典有机杂质、工艺相关杂质和降解产物

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-10 DOI: 10.1002/bmc.70021

Abhishek Sharma, Narayana Reddy Pedavenkatagari, Bhujanga Rao Nagulancha, Narasimha Swamy Lakka

{"title":"Separation and Quantitative Estimation of Pharmacopeial Organic Impurities, Process-Related Impurities, and Degradation Products of the Anti-Schizophrenia Drug Clozapine in Pharmaceutical Formulations by Using Stability-Indicating HPLC Method","authors":"Abhishek Sharma, Narayana Reddy Pedavenkatagari, Bhujanga Rao Nagulancha, Narasimha Swamy Lakka","doi":"10.1002/bmc.70021","DOIUrl":"https://doi.org/10.1002/bmc.70021","url":null,"abstract":"<div>\u0000 \u0000 <p>Clozapine is an antipsychotic medication primarily used to treat severe schizophrenia symptoms. This research aimed to develop and validate a reliable, rapid, and sensitive reverse-phase high-performance liquid chromatography (RP-HPLC) method for measuring organic impurities in clozapine drug substances and products. The chromatographic separation was achieved using an Agilent Poroshell 120 EC-C<sub>18</sub> column with gradient elution, using mobile phases consisting of acetonitrile, methanol, pH 2.4 phosphate buffer, and ethanol. The method operated at a flow rate of 1.0 mL/min, with a 10-μL injection volume, a column temperature of 35°C, and detection at 257 nm. Validation was performed according to ICH Q2(R2) guidelines and USP <1225> general chapter. The method demonstrated optimal resolution between adjacent peaks, with values greater than 1.5, and provided accurate estimations free from interference. Recovery and regression values ranged from 80.0% to 120.0%, with <i>R</i><sup>2</sup> values exceeding 0.9995. The quantification range spanned from the limit of quantification to 150% of the specification level. The stability-indicating capability of the RP-HPLC method was confirmed through forced degradation studies. This method has been successfully implemented in a quality control laboratory for real-time analysis of clozapine drug substances and products.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143380143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Continued Mismeasurement of Plasma Serotonin: A Systematic Review 血浆血清素的持续错误测量：一项系统综述

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-10 DOI: 10.1002/bmc.70016

Saketh Pillalamarri, George M. Anderson

{"title":"The Continued Mismeasurement of Plasma Serotonin: A Systematic Review","authors":"Saketh Pillalamarri, George M. Anderson","doi":"10.1002/bmc.70016","DOIUrl":"https://doi.org/10.1002/bmc.70016","url":null,"abstract":"<div>\u0000 \u0000 <p>Studies reporting on the analysis of free plasma (platelet-poor plasma, PPP) serotonin (5-hydroxytryptamine, 5-HT) in plasma obtained from healthy humans have been systematically reviewed. The review covered the period from 2010 to July 2024 and is a follow-up of a similar review published in 2011 which found that nearly all published reported of PPP 5-HT were clearly and markedly erroneously high. This problem has persisted unabated with nearly all retrieved 47 reports from the past 14 years also apparently being erroneously high. Possible causes and consequences of the problem are discussed along with potential approaches to improving the analysis and reporting of plasma 5-HT. Most of the errors appear to arise from pre-analytical problems that occur during the preparation of PPP due to residual platelets and/or due to the release of platelet 5-HT. The large number of fields interested in plasma 5-HT and the disparate publication venues appear to have contributed to a general lack of awareness of the difficulties with analyzing plasma 5-HT. The reporting of erroneous plasma 5-HT values has led to unsupported conclusions about possible alterations in plasma 5-HT and about the role of plasma 5-HT across a wide range of biomedical research areas.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143380141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research Progress on the Material Basis of Traditional Chinese Medicines Based on Chemical Biology Methods 基于化学生物学方法的中药物质基础研究进展

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-07 DOI: 10.1002/bmc.70004

Xinyue Bai, Huimin Lin, Ziqi Yang, Fan He, Jianru Chen, Hua Zhou, Yufeng Huang

引用次数: 0

Chiral Lead Halide Perovskites in Action: Unlocking Enantiomer Separation Puzzle 手性卤化铅钙钛矿的作用：解开对映体分离难题

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-07 DOI: 10.1002/bmc.6085

Pallavi Singh, Rudra Mukherjee, Anil Kumar

{"title":"Chiral Lead Halide Perovskites in Action: Unlocking Enantiomer Separation Puzzle","authors":"Pallavi Singh, Rudra Mukherjee, Anil Kumar","doi":"10.1002/bmc.6085","DOIUrl":"https://doi.org/10.1002/bmc.6085","url":null,"abstract":"<div>\u0000 \u0000 <p>Effective enantiomer separation is vital in many important sectors like pharmaceuticals, agrochemicals, food safety, and biomedical imaging, yet conventional methods are costly, slow, and chemical intensive. This has sparked interest in exploring novel materials like chiral lead halide perovskite nanocrystals to address these challenges. This newly emerging chiral material combines the superior properties of traditional halide perovskites with the unique attributes of chirality, resulting in distinct optoelectronic behaviors. This perspective provides a discussion on future research opportunities in the usage of these chiral LHP NCs for enantiomeric recognition and separation. LHPs exhibit extraordinary photophysical properties, easier surface functionalization, range of bonding interactions, and high surface area to volume ratio that can be used for detecting enantiomers. To the best of our knowledge, the use of chiral halide perovskites for the chiral discrimination of enantiomers has been scarcely reported, presenting a novel opportunity to explore their potential in enantiomeric recognition and separation.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0