LC-MS/MS同时测定丁螺环酮、阿普唑仑、氯硝西泮、地西泮和劳拉西泮:使用AGREE工具评价绿色度

IF 1.8 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Aykut Kul, Selen Al, Demet Dincel, Olcay Sagirli
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引用次数: 0

摘要

抗焦虑药物如丁螺环酮、阿普唑仑、氯硝西泮、地西泮和劳拉西泮用于治疗焦虑症。药物血药浓度的测定对患者的治疗很重要。本研究建立了一种快速、可靠、具有成本效益的LC-MS/MS方法,用于定量人血浆中丁螺环酮、阿普唑仑、氯硝西泮、地西泮和劳拉西泮的治疗药物监测。此外,符合国际统一委员会(ICH) M10生物分析方法验证和研究样本分析确保了该方法的可靠性。在进入LC-MS/MS系统前,通过盐辅助液-液微萃取(salme)从血浆中提取分析物和内标物。测定丁螺环酮、阿普唑仑、氯硝西泮、地西泮和劳拉西泮的定量下限(LLOQ)分别为0.5、2.5、2、30和10 ng/mL。丁螺环酮的校准曲线范围为0.5 ~ 50 ng/mL,阿普唑仑为2.5 ~ 250 ng/mL,氯硝西泮为2 ~ 200 ng/mL,地西泮为30 ~ 3000 ng/mL,劳拉西泮为10 ~ 1000 ng/mL,相关系数为0.99。该方法用于实际患者体内药物浓度的测定,证明了其对治疗药物监测的适用性。开发的方法已被观察到实现高分析绿色度量方法和软件(同意)得分的绿色概况。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Simultaneous Determination of Buspirone, Alprazolam, Clonazepam, Diazepam, and Lorazepam by LC-MS/MS: Evaluation of Greenness Using AGREE Tool

Anxiolytic drugs such as buspirone, alprazolam, clonazepam, diazepam, and lorazepam are used to treat anxiety disorders. Measurement of drug plasma concentrations is important in the treatment of the patient. This research developed a rapid, reliable, and cost-effective LC-MS/MS method to quantify buspirone, alprazolam, clonazepam, diazepam, and lorazepam in human plasma for therapeutic drug monitoring. Furthermore, compliance with the International Council for Harmonisation (ICH) M10 Bioanalytical Method Validation and Study Sample Analysis ensured the method's reliability. Before injection into the LC-MS/MS system, the analytes and an internal standard were extracted from plasma through salt-assisted liquid–liquid microextraction (SALLME). The lower limit of quantification (LLOQ) was determined as 0.5, 2.5, 2, 30, and 10 ng/mL for buspirone, alprazolam, clonazepam, diazepam, and lorazepam, respectively. The calibration curve ranges are 0.5–50 ng/mL for buspirone, 2.5–250 ng/mL for alprazolam, 2–200 ng/mL for clonazepam, 30–3000 ng/mL for diazepam, and 10–1000 ng/mL for lorazepam, respectively, with correlation coefficients > 0.99. Its suitability for therapeutic drug monitoring was demonstrated using the method to determine drug concentration levels in real patients. The developed method has been observed to achieve a high analytical greenness metric approach and software (AGREE) score for the greenness profile.

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来源期刊
Biomedical Chromatography
Biomedical Chromatography 生物-分析化学
CiteScore
3.60
自引率
5.60%
发文量
268
审稿时长
2.3 months
期刊介绍: Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.
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