Biomedical Chromatography最新文献

Mechanism of Baxian Huazhuo Decoction in the Treatment of Gouty Arthritis Based on Network Pharmacology, Molecular Docking, and Experimental Verification 八仙化浊汤治疗痛风性关节炎的机制——基于网络药理学、分子对接及实验验证

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-04-16 DOI: 10.1002/bmc.70456

Liting Mu, Xin Guo, Shiyuan Sun, Chen Ma, Jiayao Wang, Changhai Sun, Hongbin Qiu

{"title":"Mechanism of Baxian Huazhuo Decoction in the Treatment of Gouty Arthritis Based on Network Pharmacology, Molecular Docking, and Experimental Verification","authors":"Liting Mu, Xin Guo, Shiyuan Sun, Chen Ma, Jiayao Wang, Changhai Sun, Hongbin Qiu","doi":"10.1002/bmc.70456","DOIUrl":"https://doi.org/10.1002/bmc.70456","url":null,"abstract":"<div>\u0000 \u0000 <p>The global prevalence of gout continues to rise. Baxian Huazhuo Decoction (BHD) has demonstrated significant efficacy in the clinical treatment of acute gouty arthritis (AGA); however, its mechanism of action remains unclear. This study first employed network pharmacology analysis to identify the key components, targets, and pathways of BHD against AGA. Molecular docking studies validated the binding affinity between the components of BHD and their potential targets. Ultrahigh-performance liquid chromatography–high-resolution mass spectrometry (UHPLC–HRMS) was utilized to identify the active components in BHD and elucidate their fragmentation pathways. Subsequently, a monosodium urate crystal–induced AGA rabbit model was established to evaluate the in vivo therapeutic efficacy of BHD. The results revealed 62 predicted active components and 268 target molecules in BHD, identifying core constituents such as gentiopicroside, limonin, and indirubin, which exhibited high affinity for targets including MAPK1, PPARG, and IL-6. In vivo experiments confirmed that BHD significantly suppressed the phosphorylation of MAPK1, reduced the levels of pro-inflammatory factors such as TNF-α and IL-6, mitigated synovial damage, and inhibited the activation of the PI3K-Akt signaling pathway. This study systematically elucidates the pharmacological basis and mechanisms of action of BHD in the treatment of AGA, providing a scientific basis for its clinical application.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 6","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147686007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Untargeted Metabolomic Study of the Anti-HBV Effects of Erythrocentaurin In Vitro and In Vivo Using UPLC-Q-TOF/MS UPLC-Q-TOF/MS非靶向代谢组学研究红centaurin体外和体内抗hbv作用

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-04-16 DOI: 10.1002/bmc.70447

Yige Wang, Shuhan Tang, Pengyu Li, Yidan Sun, Yaqi Xu, Hao Li, HongYing Xu, Ying Wang, Hailong Zhang, Xianna Li, Zhigang Wang

{"title":"Untargeted Metabolomic Study of the Anti-HBV Effects of Erythrocentaurin In Vitro and In Vivo Using UPLC-Q-TOF/MS","authors":"Yige Wang, Shuhan Tang, Pengyu Li, Yidan Sun, Yaqi Xu, Hao Li, HongYing Xu, Ying Wang, Hailong Zhang, Xianna Li, Zhigang Wang","doi":"10.1002/bmc.70447","DOIUrl":"https://doi.org/10.1002/bmc.70447","url":null,"abstract":"<div>\u0000 \u0000 <p>Erythrocentaurin is a key bioactive metabolite derived from the intestinal biotransformation of swertiamarin and gentiopicroside, two predominant iridoid glycosides in <i>Swertia</i> herbs that have antihepatitis B virus activity. HBV-Tg mice and HepG2.2.15 cells were used as in vivo and in vitro models to detect HBsAg and HBeAg, and also determine HBV DNA levels, by conducting ELISA and qPCR. We also performed untargeted metabolomics via UPLC-Q-TOF/MS. Moreover, the contents of NF-κB pathway–related genes and proteins were measured through conducting qRT-PCR and Western blotting assays, and P65 nuclear translocation was examined via immunofluorescence assays. The results revealed that erythrocentaurin significantly inhibited the replication of HBV, reversed 55 differentially abundant metabolites, core-regulated glutathione and arachidonic acid metabolism, and suppressed the NF-κB pathway by downregulating p-IκK/p-P65, upregulating IκB, and inhibiting the nuclear translocation of P65. This study provided theoretical support and broad prospects for developing new anti-HBV monomer drugs and offered a novel model that can be used for identifying pharmacodynamic substances and the secondary development of traditional Chinese medicine formulae and single herbs.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 6","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147686048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A High-Throughput LC–MS/MS Bioanalytical Method for Simultaneous Determination of Mirabegron and Solifenacin in Pharmacokinetic Investigations 高通量LC-MS /MS生物分析方法同时测定Mirabegron和Solifenacin的药动学研究

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-04-15 DOI: 10.1002/bmc.70425

Sadakar Tungapati, Srinivasu Nuvuluri, M. Ramakrishna

{"title":"A High-Throughput LC–MS/MS Bioanalytical Method for Simultaneous Determination of Mirabegron and Solifenacin in Pharmacokinetic Investigations","authors":"Sadakar Tungapati, Srinivasu Nuvuluri, M. Ramakrishna","doi":"10.1002/bmc.70425","DOIUrl":"https://doi.org/10.1002/bmc.70425","url":null,"abstract":"<div>\u0000 \u0000 <p>Mirabegron, a Beta-3 adrenoceptor agonist drug, and Solifenacin succinate, an antimuscarinics drug, are used to treat overactive bladder in combination. Till today, no bioanalytical method has been reported to estimate Mirabegron and Solifenacin succinate in type of matrix. Mirabegron and Solifenacin succinate were measured in rat plasma using Darifenacin as the internal standard, a newly developed and validated bioanalytical LC–MS/MS technique. The analytes were separated using a Luna C18 column and a mobile phase combination of acetonitrile: 0.1% formic acid in HPLC water (50:50 v/v). The flow rate was maintained at an isocratic level of 1.0 mL/min, and the runtime was about 5 min. Mirabegron (m/z 397.51 to 83.78) and Solifenacin succinate (m/z 481.56 to 148.79) were determined by using the mass spectra with positive mode of multiple reaction monitoring. The strategy was approved in accordance with USFDA regulations. Accuracy with mean % recovery of 96.25% to 98.45% and 96.46% to 98.05% and strong linearity with <i>r</i><sup>2</sup> value of 0.9998 in the ranges of Mirabegron (2.5–100 ng/mL) and Solifenacin succinate (0.5–20 ng/mL) are the outcomes. All other parameters fall within the approved range. Indicators of medication effectiveness and safety, pharmacokinetic parameters, may be determined using the proposed technique.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 6","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147686314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of Encapsulated and Unencapsulated Amphotericin B in Dog Plasma by LC–MS/MS Coupled With a Simple and Efficient Solid-Phase Extraction: Application to a Pharmacokinetic Study of Liposomal Amphotericin B 高效液相色谱-质谱联用固相萃取法测定犬血浆中两性霉素B包封和未包封的含量：两性霉素B脂质体药动学研究

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-04-09 DOI: 10.1002/bmc.70444

Haiyan Xu, Jing Zhuo, Huanyu Wei, Ruihan Ma, Xingyue Zhang, Aiping Yu, Yanan Zhang, Xiumei Lu

{"title":"Determination of Encapsulated and Unencapsulated Amphotericin B in Dog Plasma by LC–MS/MS Coupled With a Simple and Efficient Solid-Phase Extraction: Application to a Pharmacokinetic Study of Liposomal Amphotericin B","authors":"Haiyan Xu, Jing Zhuo, Huanyu Wei, Ruihan Ma, Xingyue Zhang, Aiping Yu, Yanan Zhang, Xiumei Lu","doi":"10.1002/bmc.70444","DOIUrl":"10.1002/bmc.70444","url":null,"abstract":"<div>\u0000 \u0000 <p>A convenient and reliable SPE method coupled with a specific and sensitive LC–MS/MS technique was developed and validated for the separation and determination of unencapsulated amphotericin B (F-AMB) and encapsulated amphotericin B (L-AMB) in dog plasma. L-AMB and F-AMB in the biomatrix were simultaneously separated by Oasis HLB SPE column using natamycin as the internal standard (IS). Chromatographic separation was achieved on a ZORBAX Eclipse XDB C18 column with gradient elution at a flow rate of 0.5 mL/min. The mobile phase consisted of methanol (0.1% formic acid) and a 5-mM ammonium acetate solution (0.1% formic acid). Mass spectrometry detection was performed in positive ion mode with an electrospray ionization source. Apart from regular quality control (QC) samples, a series of cross-QCs was adopted to verify the specificity and reproducibility of the quantification. We showed that F-AMB and L-AMB were completely separated without mutual interference in the quantitative linearity ranges of F-AMB and L-AMB, which were 10.0–800 and 100–25,000 ng/mL, respectively. The method was then applied to a pharmacokinetic study of liposomal amphotericin B in beagle dogs, and excellent ISR results were obtained for both L-AMB and F-AMB assays.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 5","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147637904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Validation of an LC–MS/MS Method for Quantitative Determination of Tacrolimus in Mouse Serum LC-MS/MS法测定小鼠血清中他克莫司含量的建立与验证。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-04-08 DOI: 10.1002/bmc.70437

Xiaoming Wang, Madhuri Tatiparthy, Tilu Jain Thomas, Vinoshana Sama, Ramkumar Menon, Ananth Kumar Kammala

{"title":"Development and Validation of an LC–MS/MS Method for Quantitative Determination of Tacrolimus in Mouse Serum","authors":"Xiaoming Wang, Madhuri Tatiparthy, Tilu Jain Thomas, Vinoshana Sama, Ramkumar Menon, Ananth Kumar Kammala","doi":"10.1002/bmc.70437","DOIUrl":"10.1002/bmc.70437","url":null,"abstract":"<div>\u0000 \u0000 <p>Tacrolimus, a potent immunosuppressant with a narrow therapeutic index, is a known substrate of P-glycoprotein (P-gp), a key efflux transporter involved in drug disposition. Accurate and sensitive quantification of tacrolimus in small-volume mouse serum is essential not only for pharmacokinetic studies but also for evaluating in vivo P-gp functional activity, particularly in pregnancy-related drug transport research. Tacrolimus was extracted using methyl tert-butyl ether after alkalization of the serum with NH₄OH. Chromatographic separation was achieved on a C<sub>18</sub> HPLC column with a mobile phase of acetonitrile and water containing 0.2% NH₄OH. Detection was performed in negative ion mode using multiple reaction monitoring (MRM) transitions of <i>m</i>/<i>z</i> 802.5 → 560.6 for tacrolimus and <i>m</i>/<i>z</i> 805.5 → 563.6 for the internal standard, tacrolimus-<sup>13</sup><i>C</i>,<i>d</i><sub><i>2</i></sub>. The calibration range for tacrolimus was 0.26–44.8 ng/mL. The method demonstrated good precision, with intra- and inter-day relative standard deviations below 12%, and accuracy ranging from 94% to 103%. Compared to previously published methods, this approach requires only 200 μL of mouse serum and provides high sensitivity, with a lower limit of quantification of 0.26 ng/mL. The assay showed high sensitivity, minimal matrix interference, and good stability across tested conditions.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 5","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147637964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomics Reveals the Mechanism of American Ginseng in Alleviating Insulin Resistance by Reversing Metabolic Disorders in HepG2 Cells 代谢组学揭示西洋参通过逆转HepG2细胞代谢紊乱缓解胰岛素抵抗的机制

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-04-05 DOI: 10.1002/bmc.70441

Ye Jing, Sixuan Wang, Yu Geng, Hao Lu, Yulin Dai, Fei Zheng, Yang Wang, Xin Huang, Hao Yue, Yunlong Guo

{"title":"Metabolomics Reveals the Mechanism of American Ginseng in Alleviating Insulin Resistance by Reversing Metabolic Disorders in HepG2 Cells","authors":"Ye Jing, Sixuan Wang, Yu Geng, Hao Lu, Yulin Dai, Fei Zheng, Yang Wang, Xin Huang, Hao Yue, Yunlong Guo","doi":"10.1002/bmc.70441","DOIUrl":"10.1002/bmc.70441","url":null,"abstract":"<div>\u0000 \u0000 <p>American ginseng (<i>Panax quinquefolium</i> L.) is a traditional Chinese medicinal herb that has been used in China for hundreds of years. The significant hypoglycemic activity of American ginseng has made it widely used in health food as a valuable medicinal herb with homologous origin. However, its effect and mechanism of improving insulin resistance (IR) remain unclear. In the present study, an IR-HepG2 cells model induced by high concentrations of insulin was constructed and treated with ethanol extract of dried American ginseng (EDAG) at different concentrations to reveal the in vitro ameliorative effect of EDAG on IR by monitoring glucose consumption, glycogen content, triglyceride (TG) content, and total cholesterol (TC) content of the cells. The cell metabolite changes were tracked by liquid chromatography-mass spectrometry (LC-MS)-based metabolomics combined with multivariate statistical analysis, the metabolic biomarkers and related metabolic pathways were analyzed, and further elucidate its mechanism of action. The results showed that treatment with EDAG increased glucose consumption and glycogen content, and decreased intracellular TC and TG content in IR-HepG2 cells to different degrees. The results of cell metabolomics indicated that EDAG treatment reversed metabolic disorders by regulating sphingolipid metabolism, linoleic acid metabolism, and arginine and proline metabolism, etc. The present study explored the in vitro IR improvement mechanism of EDAG based on the LC-MS cell metabolomics approach, which confirms the great potential of LC-MS technology in the evaluation of drug efficacy and can be an effective tool for the related analysis of other Chinese herbal medicines.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 5","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analytical Challenges and Emerging Detection Strategies of Synthetic Cannabinoids in Horse Doping Control 合成大麻素在赛马兴奋剂控制中的分析挑战和新兴检测策略。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-04-05 DOI: 10.1002/bmc.70432

Erol Kabil, Mehmet Nihat Ural, Eylem Funda Göktaş

{"title":"Analytical Challenges and Emerging Detection Strategies of Synthetic Cannabinoids in Horse Doping Control","authors":"Erol Kabil, Mehmet Nihat Ural, Eylem Funda Göktaş","doi":"10.1002/bmc.70432","DOIUrl":"10.1002/bmc.70432","url":null,"abstract":"<div>\u0000 \u0000 <p>Synthetic cannabinoids (SCs) were originally synthesized to advance the understanding of the endocannabinoid system, facilitate disease research, and support the development of novel therapeutic agents. Compared with tetrahydrocannabinol, these compounds exhibit substantially higher psychoactive potency and enhanced receptor-binding affinity. The rapid and continuous evolution of SC derivatives presents significant challenges for analytical laboratories and increases the risk of misuse. Furthermore, SCs may be exploited to alter performance in both human and animal sports. The scarcity of comprehensive data regarding their toxicity, pharmacokinetics, and potential performance-enhancing mechanisms has made SCs a priority concern in recent years. This concern spans public health, forensic science, animal welfare, and sports integrity. This review examines the historical development, pharmacological properties, chemical characteristics, and physiological effects of SCs. It also presents an overview of sample preparation strategies and analytical methodologies applied to various biological matrices. Furthermore, it highlights the limited number of studies on the administration and detection of SCs in racehorses and offers a comprehensive assessment of future perspectives.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 5","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeted LC–MS/MS-Based Serum Nucleoside Metabolomics for Early Detection and Biomarker Discovery in Colorectal Cancer 靶向LC-MS/MS-Based血清核苷代谢组学用于结直肠癌的早期检测和生物标志物发现。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-04-02 DOI: 10.1002/bmc.70439

Ramzy Rashed, Mostafa Abdelglil, Ghada Salama

{"title":"Targeted LC–MS/MS-Based Serum Nucleoside Metabolomics for Early Detection and Biomarker Discovery in Colorectal Cancer","authors":"Ramzy Rashed, Mostafa Abdelglil, Ghada Salama","doi":"10.1002/bmc.70439","DOIUrl":"10.1002/bmc.70439","url":null,"abstract":"<div>\u0000 \u0000 <p>Colorectal cancer (CRC) is a leading cause of cancer-related morbidity and mortality worldwide, and early detection remains essential for improving patient outcomes. This study evaluated the diagnostic potential of targeted serum nucleoside metabolomic profiling, alone and in combination with established tumor markers (CEA and CA19-9). Serum samples from 50 CRC patients and 25 healthy controls were analyzed using a validated LC–MS/MS platform. Sample preparation and analytical conditions were optimized using a design of experiment (DoE) approach. Multivariate analysis was performed using partial least squares–discriminant analysis (PLS-DA), and diagnostic performance was assessed using receiver operating characteristic (ROC) curve analysis. CRC patients showed increased purine metabolites and modified nucleosides (adenosine, inosine, xanthine, hypoxanthine, and N<sup>6</sup>-methyladenosine) and decreased cytidine and uridine (<i>p</i> < 0.01). The metabolomic panel showed strong discrimination (AUC = 0.91; sensitivity = 88%; specificity = 86%). Conventional markers showed moderate performance (CEA: AUC = 0.83; CA19-9: AUC = 0.79). Combined analysis improved performance (AUC = 0.95; sensitivity = 92%; specificity = 89%). Targeted serum nucleoside profiling enhances CRC detection and improves diagnostic accuracy when integrated with conventional tumor markers.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 5","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Multidimensional Evaluation of Quality Markers in Yupingfeng via UPLC-Q-Orbitrap-MS, Network Pharmacology, UPLC-UV Fingerprint, and UPLC-QQQ-MS UPLC-Q-Orbitrap-MS、网络药理学、UPLC-UV指纹图谱和UPLC-QQQ-MS对玉屏风药材质量标记物的鉴定及多维评价

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-04-02 DOI: 10.1002/bmc.70435

Shu Li, Xiaoyu Jin, Huasheng Hu, Xiaodan Wang, Hui Chen, Jian Jin, Ao Xu

{"title":"Identification and Multidimensional Evaluation of Quality Markers in Yupingfeng via UPLC-Q-Orbitrap-MS, Network Pharmacology, UPLC-UV Fingerprint, and UPLC-QQQ-MS","authors":"Shu Li, Xiaoyu Jin, Huasheng Hu, Xiaodan Wang, Hui Chen, Jian Jin, Ao Xu","doi":"10.1002/bmc.70435","DOIUrl":"10.1002/bmc.70435","url":null,"abstract":"<div>\u0000 \u0000 <p>Yupingfeng (YPF), a classical traditional Chinese medicine (TCM) formula, is widely used to tonify qi and strengthen the exterior. However, research on the quality control of YPF remains limited. A single indicator is insufficient to comprehensively reflect the quality attributes of this complex TCM system. This study aims to investigate the potential quality markers (Q-markers) of YPF with multidimensional analysis. First, 28 specific components of YPF were preliminarily identified by UPLC-Q-Orbitrap-MS/MS based on ingredient specificity. Next, the targets of specific ingredients were predicted using SwissTargetPrediction and analyzed with protein–protein interaction network analysis to obtain 71 core targets. The effectiveness was prioritized through systematic ranking based on network pharmacological analysis. Then, common components shared by YPF powder, YPF preparations, and rat serum were characterized using UPLC-QQQ-MS and UPLC-UV. Finally, 10 compounds, including prim-O-glucosylcimifugin, calycosin-7-O-glucopyranoside, cimifugin, 5-O-methylvisammioside, ononin, sec-O-glucosylhamaudol, calycosin, astragaloside A, formononetin, and atractylenolide II, were identified as the Q-markers of YPF, and a rapid UPLC-QQQ-MS method was developed for their simultaneous determination. Overall, the multidimensional analysis based on the “five principles” was first applied to identify the Q-markers of YPF, thereby providing guidance for the quality control of YPF and its preparations.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 5","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Establishment of LC–MS/MS Method for Quantitative Analysis of Pharmacokinetics and Tissue Distribution of Trilobatin—Comparison of Three Administration Modes 首次建立三叶草药动学及组织分布定量分析的LC-MS/MS方法——三种给药方式的比较。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-04-01 DOI: 10.1002/bmc.70426

Xiaojing Wang, Cong Hu, Peifang Song, Ling Yang

{"title":"First Establishment of LC–MS/MS Method for Quantitative Analysis of Pharmacokinetics and Tissue Distribution of Trilobatin—Comparison of Three Administration Modes","authors":"Xiaojing Wang, Cong Hu, Peifang Song, Ling Yang","doi":"10.1002/bmc.70426","DOIUrl":"10.1002/bmc.70426","url":null,"abstract":"<div>\u0000 \u0000 <p>Trilobatin is a novel dihydrochalcone natural food additive. It has multiple functions such as anti-inflammatory, antioxidant, and anticancer effects. This study first developed and validated a method based on liquid chromatography–tandem mass spectrometry for the quantitative determination of trilobatin in rat plasma and tissues. The method demonstrated high precision, high accuracy, good extraction recovery, and minimal matrix effects. Subsequently, this method was used to study the pharmacokinetics and tissue distribution of trilobatin after oral, intravenous, and intraperitoneal administration in rats. Pharmacokinetic analysis showed that trilobatin was rapidly absorbed after oral administration with a <i>T</i><sub>max</sub> of 1 h, and <i>T</i><sub>max</sub> was also approximately 1 h after intraperitoneal administration. Compared to intravenous injection, the relative bioavailability of oral administration and intraperitoneal injection is only 0.004% and 0.3%, respectively. Tissue distribution results from the three administration routes indicated that trilobatin exhibits widespread tissue distribution. These findings provide a theoretical basis for further research on trilobatin. This study provides detailed insights into the pharmacokinetic and tissue distribution characteristics of trilobatin in rats for the first time, laying the foundation for further research on trilobatin as a potential new drug candidate.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 5","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0