Biomedical Chromatography最新文献

Novel LC-MS/MS Method for Quantification of Famotidine and Metoprolol and Application to Pharmacokinetic Interaction 新型LC-MS/MS法莫替丁和美托洛尔的定量分析及药动学相互作用研究

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-19 DOI: 10.1002/bmc.70114

Ran Xu, En-Fu Feng, Sun-Jun Yin, Ping Wang, Rui Meng, Xue-Sha Zhang, Jian-Mei Pan, Gong-Hao He

{"title":"Novel LC-MS/MS Method for Quantification of Famotidine and Metoprolol and Application to Pharmacokinetic Interaction","authors":"Ran Xu, En-Fu Feng, Sun-Jun Yin, Ping Wang, Rui Meng, Xue-Sha Zhang, Jian-Mei Pan, Gong-Hao He","doi":"10.1002/bmc.70114","DOIUrl":"https://doi.org/10.1002/bmc.70114","url":null,"abstract":"<div>\u0000 \u0000 <p>A rapid and sensitive LC-MS/MS method was developed and validated to simultaneously determine famotidine (FAM) and metoprolol (MET) in rat plasma and applied to study the pharmacokinetic drug–drug interaction between these two drugs in rats. In this method, D4-famotine (D4-FAM) and D6-metoprolol (D6-MET) were used as the internal standard and methanol protein precipitation method was used for sample preparation. After extraction, the samples were carried on an Agilent Gemini-NX C<sub>18</sub> column and subjected to a gradient elution process using a mixture of methanol and water containing 0.1% formic acid at a flow rate of 0.4 mL/min within 8 min. The monitored transitions were m/z 338.1 → 189.1 for FAM, 268.2 → 116.1 for MET, 342.1 → 190 for D4-FAM, and 274.2 → 122.1 for D6-MET. The analytes had good linearity in the range of 1–200 ng/mL for FAM and 1–400 ng/mL for MET, with the lower limit of quantitation of 1 ng/mL for both drugs. The validated method was verified to meet the determination requirements of biological samples. It was the first time to study the pharmacokinetics interaction between FAM and MET successfully, which would be necessary and beneficial to explore the clinical safety and efficacy of the combination of these two drugs in the treatment of HF.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validated HPLC Method for the Pharmacokinetic Study of Linezolid in Rats 高效液相色谱法研究利奈唑胺在大鼠体内的药动学

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-19 DOI: 10.1002/bmc.70107

Jin-yao Liu, Ying-ying Pang, Piao-piao Wang, Wen-yan Li, Jing Dong

{"title":"Validated HPLC Method for the Pharmacokinetic Study of Linezolid in Rats","authors":"Jin-yao Liu, Ying-ying Pang, Piao-piao Wang, Wen-yan Li, Jing Dong","doi":"10.1002/bmc.70107","DOIUrl":"https://doi.org/10.1002/bmc.70107","url":null,"abstract":"<div>\u0000 \u0000 <p>Linezolid is an oxazolidinone with potent antibacterial activity against multidrug resistant Gram-positive bacteria. This study aimed to develop and validate a sensitive, rapid and reliable high-performance liquid chromatography-photodiode array detector method for determining linezolid concentrations in the plasma and urine and to further characterize the pharmacokinetics of linezolid in rats. Chromatographic separation was performed using a SinoPak BEH C<sub>18</sub> column with isocratic elution (acetonitrile: water, 28:72, v/v). The retention times of linezolid and the internal standard are 3.30 and 5.20 min, respectively. The calibration curve for linezolid was linear over the concentration range of 0.25–50.0 μg/mL in rat plasma and 1.00–100.0 μg/mL in rat urine. Intra- and inter-assay precision values were below 9.00%, with accuracy ranging from 0.67% to 8.34%. Plasma samples were extracted using acetonitrile-mediated protein precipitation, and the recovery was > 93%. Following intragastric (63 mg/kg) and intravenous (25 mg/kg) administration of linezolid to rats, the area under the plasma concentration–time profile from time 0 to infinity were 224.5 and 86.4 μg·h/mL, total clearance were 0.29 and 0.30 L/h/kg, respectively. The cumulative urinary excretion rates of linezolid were 26.2%–27.5% for both administration routes. The presented method can certainly be used for routine analysis of linezolid in plasma and urine.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Quantification of Methylene Blue and Evaluation of Its Pharmacokinetics in ICR Mice by Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry Using Difluoroacetic Acid” 对“二氟乙酸液相色谱-四极杆飞行时间质谱法测定亚甲基蓝及其在ICR小鼠体内药代动力学评价”的修正

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-16 DOI: 10.1002/bmc.70116

引用次数: 0

Gut Microbiota Combined With Metabolomics to Reveal the Mechanism of Tang Wang Ming Mu Granule in the Treatment of Diabetic Retinopathy in Mice 肠道菌群联合代谢组学揭示汤王明目颗粒治疗小鼠糖尿病视网膜病变的机制

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-16 DOI: 10.1002/bmc.70112

Hong Xing, Yu-Jie Ding, Yuan Zhang, Ning-Ning Li, Shu-Zhen Hou, Er-Wei Liu, Xiao-Peng Chen

{"title":"Gut Microbiota Combined With Metabolomics to Reveal the Mechanism of Tang Wang Ming Mu Granule in the Treatment of Diabetic Retinopathy in Mice","authors":"Hong Xing, Yu-Jie Ding, Yuan Zhang, Ning-Ning Li, Shu-Zhen Hou, Er-Wei Liu, Xiao-Peng Chen","doi":"10.1002/bmc.70112","DOIUrl":"https://doi.org/10.1002/bmc.70112","url":null,"abstract":"<div>\u0000 \u0000 <p>Diabetes retinopathy (DR) is one of the serious complications of diabetes. Clinical practice has proved that Tang Wang Ming Mu Granule (TWMM) can improve symptoms of DR patients. The mechanism of TWMM in treating DR in mice was studied, combining gut microbiota with metabolomics. A high-fat and high-sugar diet combined with streptozotocin (STZ) injection was used to create a mouse model of DR. The C57BL6/J wild-type mice were divided into five groups, including normal control, DR model, TWMM (2.7 and 10.8 g/kg) treatment, and the positive control treatment groups. Based on urine metabolomics and 16S rDNA sequencing of fecal samples, the effects of TWMM on host metabolism and intestinal microbiota were studied. The results showed that TWMM reverses the disordered intestinal flora to normal. In addition, the pathway prediction of intestinal microorganisms was related to the metabolic pathways. Meanwhile, the metabolomics analysis found that the differential metabolites were mainly concentrated in amino acids and their metabolites, carbohydrates, and their metabolites. The Shigellosis pathway attracted attention, and <i>Shigella</i> shows good indication in the treatment. The research provides a method for metabolic disease study with gut microbiota combined with metabolomics and treatment targets and pathways of DR.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Active Ingredients of Hepatoprotective Effect of Raw and Stir-Baked Gardeniae Fructus Based on Spectrum–Effect Relationship Analysis 基于谱效关系分析的生炒栀子保肝活性成分研究

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-16 DOI: 10.1002/bmc.70115

Yangang Cao, Shujing Li, Peiyang Zhang, Hongwei Li, Zhiyou Hao, Xiaoke Zheng, Weisheng Feng

{"title":"Exploring Active Ingredients of Hepatoprotective Effect of Raw and Stir-Baked Gardeniae Fructus Based on Spectrum–Effect Relationship Analysis","authors":"Yangang Cao, Shujing Li, Peiyang Zhang, Hongwei Li, Zhiyou Hao, Xiaoke Zheng, Weisheng Feng","doi":"10.1002/bmc.70115","DOIUrl":"https://doi.org/10.1002/bmc.70115","url":null,"abstract":"<div>\u0000 \u0000 <p>Gardeniae Fructus (GF) has been used as a hepatoprotective medicine; however, the active ingredients of GF against cholestatic liver injury (CLI) remain unclear. This study aims to explore active ingredients of the hepatoprotective effects of raw GF (RGF) and stir-baked GF (SGF) by spectrum–effect relationship analysis. A total of 32 common peaks were recorded in RGF and SGF HPLC fingerprints, and nine of them were structurally characterized. Both RGF and SGF demonstrated hepatoprotective effects in an ANIT-induced CLI rat model. The spectrum–effect relationship analysis results showed that peaks 3 (gardenoside), 4, 5 (jasminoside B), 6 (genipin 1-gentiobioside), 8 (geniposide), 9, 16, 17, 19, and 22 were determined as the potential active ingredients of GF against CLI. Notably, gardenoside, jasminoside B, and genipin 1-gentiobioside exhibited good hepatoprotective effects. The research establishes a research foundation for the future quality control and medicinal application of GF.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-Targeted Metabolomics Analysis of Metabolite Differences in Inocutis tamaricis Under Different Culture Conditions 不同培养条件下柽柳链球菌代谢物差异的非靶向代谢组学分析

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-16 DOI: 10.1002/bmc.70113

Na Zhang, Qi Cui, Xiao-Feng Ma, Kai Huang, Qin Tian, Tian-Li Zhang, Guo-Chao Wu, Shu-De Yang, Xian Hao Cheng, Rui Zhang, Yong-Fei Ming

{"title":"Non-Targeted Metabolomics Analysis of Metabolite Differences in Inocutis tamaricis Under Different Culture Conditions","authors":"Na Zhang, Qi Cui, Xiao-Feng Ma, Kai Huang, Qin Tian, Tian-Li Zhang, Guo-Chao Wu, Shu-De Yang, Xian Hao Cheng, Rui Zhang, Yong-Fei Ming","doi":"10.1002/bmc.70113","DOIUrl":"https://doi.org/10.1002/bmc.70113","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Inocutis tamaricis</i> is a medicinal fungus with significant pharmacological activity. In this study, ultra-high performance liquid chromatography-high resolution mass spectrometry (UPLC/MS) was used to conduct a comprehensive non-targeted metabolomics analysis of the metabolites of wild fruiting body (WF), cultured fruiting body (CF), and liquid cultured mycelium (LCM). Orthogonal partial least square discriminant analysis (OPLS-DA) was used to compare the three culture methods, to describe the regulatory effect of specific culture methods on metabolite distribution and yield of <i>I. tamaricis</i>, and the differential metabolites were enriched and analyzed to identify key metabolic pathways. The results showed significant differences in the types and abundances of active substances produced in different culture methods. There were 35 compounds in common, the WF, CF, and LCM had their unique metabolites, which were 39, 16, and 29 respectively, confirming the significant differences in the metabolites in different cultures, six key metabolic pathways were identified, including unsaturated fatty acid biosynthesis, linoleic acid, and <i>α</i>-linolenic acid metabolism, etc.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comparative Research of the Flavonoid Metabolites From Viscum coloratum in Normal and RA Rats by an Integrated Analytical Strategy

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-13 DOI: 10.1002/bmc.70105

Yu-Qing Wang, Wei Guan, Yan-Ying Li, Bo Wen, Zhi-Jiang Chen, Shuang Liu, Yan-Fu Wang, Zhi-Chao Hao, Qing-Shan Chen, Li-li Zhang, Shu Liu, Anam Naseem, Yao-Xin Sui, Si-Tong Liu, Hai-Xue Kuang, Bing-You Yang, Yan Liu

{"title":"A Comparative Research of the Flavonoid Metabolites From Viscum coloratum in Normal and RA Rats by an Integrated Analytical Strategy","authors":"Yu-Qing Wang, Wei Guan, Yan-Ying Li, Bo Wen, Zhi-Jiang Chen, Shuang Liu, Yan-Fu Wang, Zhi-Chao Hao, Qing-Shan Chen, Li-li Zhang, Shu Liu, Anam Naseem, Yao-Xin Sui, Si-Tong Liu, Hai-Xue Kuang, Bing-You Yang, Yan Liu","doi":"10.1002/bmc.70105","DOIUrl":"https://doi.org/10.1002/bmc.70105","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Viscum coloratum</i> (Kom.) Nakai has been demonstrated to be an effective treatment for rheumatoid arthritis (RA), but the pharmacodynamic substances are still unclear. In this study, a four-step strategy integrated nontargeted metabolomics, multivariate statistical analysis, and UNIFI software. An ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) method was employed to characterize 56 flavonoids from <i>V. coloratum</i>, 25 prototypes and 133 metabolites in biological samples of rats following oral administration of <i>V. coloratum</i>. The endogenous interference peaks in plasma, urine, and feces were reduced by 83.79%, 91.60%, and 86.02%, respectively, through the application of nontargeted metabolomics approaches. The distinctions and commonalities in the flavonoid metabolic pathways of <i>V. coloratum</i> under normal and RA conditions were summarized. Phase II metabolism was significantly affected in the RA rats, especially the prototype exposure and its metabolites in plasma and excreted by urine and feces. Utilizing the aforementioned methods, we identified 109 differential metabolites, including 17 RA-specific metabolites. Twenty-two flavonoid prototypes and their metabolites were identified as potential pharmacodynamic substances in plasma. All the information gained from this study will significantly contribute to elucidating the potential biological and pharmacological mechanisms of flavonoids in <i>V. coloratum</i>, thereby opening new avenues for drug development.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of Schaftoside and Isoschaftoside in Rat Plasma Utilizing UPLC-MS/MS 高效液相色谱-质谱联用法测定大鼠血浆中禾甲苷和异禾甲苷的含量

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-12 DOI: 10.1002/bmc.70106

Jianbo Li, Runrun Wang, Mengmeng Shao, Yongxi Jin, Saiya Chen, Xianqin Wang, Fang Chen

{"title":"Determination of Schaftoside and Isoschaftoside in Rat Plasma Utilizing UPLC-MS/MS","authors":"Jianbo Li, Runrun Wang, Mengmeng Shao, Yongxi Jin, Saiya Chen, Xianqin Wang, Fang Chen","doi":"10.1002/bmc.70106","DOIUrl":"https://doi.org/10.1002/bmc.70106","url":null,"abstract":"<div>\u0000 \u0000 <p>To evaluate the pharmacokinetics, absolute bioavailability, and plasma concentrations of schaftoside and isoschaftoside in rats, an UPLC-MS/MS method was employed. For sample preparation, plasma proteins were precipitated using chilled methanol. The separation was achieved on a UPLC HSS T3 column with a mobile phase consisting of methanol and water (with 0.1% formic acid in water), at a flow rate of 0.4 mL/min. Detection was performed using electrospray ionization (ESI) in positive ion mode, coupled with multiple reaction monitoring (MRM) for quantitative analysis. Rats received oral doses of schaftoside (1 mg/kg) and isoschaftoside (5 mg/kg), and the pharmacokinetic profiles of both compounds were compared. The calibration curve for the method demonstrated excellent linearity within the concentration range of 1–2000 ng/mL, with correlation coefficients (<i>r</i> values) exceeding 0.99. Following intravenous and oral administration, significant differences were observed in the AUC<sub>(0–t)</sub> between schaftoside and isoschaftoside, whereas their half-lives (t<sub>1/2</sub>) remained comparable. The absolute bioavailability of schaftoside and isoschaftoside in rat plasma was determined to be 0.95% and 0.22%, respectively.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Chemical Profiling of Moringa oleifera Leaves Extracts by LC–MS/MS Followed by In Silico ADMET Prediction Using SwissADME 用LC-MS /MS分析辣木叶提取物的化学成分，并用SwissADME进行ADMET预测

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-12 DOI: 10.1002/bmc.70110

Abdelhadi Seghir, Meriem Mokhtar, Khaldun M. Al Azzam, Bounoua Nadia, Erdi Can Aytar, Amel Saad, Touati Boumediene

{"title":"Comprehensive Chemical Profiling of Moringa oleifera Leaves Extracts by LC–MS/MS Followed by In Silico ADMET Prediction Using SwissADME","authors":"Abdelhadi Seghir, Meriem Mokhtar, Khaldun M. Al Azzam, Bounoua Nadia, Erdi Can Aytar, Amel Saad, Touati Boumediene","doi":"10.1002/bmc.70110","DOIUrl":"https://doi.org/10.1002/bmc.70110","url":null,"abstract":"<div>\u0000 \u0000 <p>This study analyses the nutritional and medicinal properties of <i>Moringa oleifera</i> leaves from sub-Saharan Africa using HPLC–PDA–ESI-MS. A method for simultaneous polyphenol quantification was developed to understand how different habitats influence the quality and polyphenolic profile of <i>M. oleifera</i>. The study specifically aimed to analyze the polyphenolic profile of phenolic compounds extracted from <i>M. oleifera</i> leaves from the Tabelbala region in Bechar, Algeria. The extract's complete polyphenolic profile was determined using liquid chromatography, photodiode array, and mass spectrometry detection via an electrospray ionization interface. A total of 16 compounds were identified, with variations observed between different extracts. The most abundant among these were quercetin-3-<i>O</i>-glucoside (964.43 μg/g dry matter), kaempferol (839.71 μg/g dry matter), and rutin (835.51 μg/g dry matter). The acetonic extract was the only source of gallic acid, which was measured at 496.14 μg/g dry matter. It provides a database for qualitative assessments and clinical applications of <i>M. oleifera</i>, laying the groundwork for future germplasm selection and development research. Quantitative analysis methodology can be applied to quality assessment protocols. Findings show compounds with low gastrointestinal absorption and skin permeability prevent CYP-related medication interactions, but poor bioavailability and efflux transport capabilities limit their therapeutic potential, necessitating formulation strategies.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143938890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Simple, Sensitive, and Stable LC–MS/MS Method for the Simultaneous Determination and Pharmacokinetic Study of Dapagliflozin and Its Metabolite D3OG in Human Plasma 一种简单、灵敏、稳定的LC-MS /MS同时测定人血浆中达格列净及其代谢物D3OG的药动学研究

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-07 DOI: 10.1002/bmc.70108

Ancheng Gu, Chuwen Zhang, Dan Li, Bohong Cen, Wanwen Cao, Zhongyuan Xu

{"title":"A Simple, Sensitive, and Stable LC–MS/MS Method for the Simultaneous Determination and Pharmacokinetic Study of Dapagliflozin and Its Metabolite D3OG in Human Plasma","authors":"Ancheng Gu, Chuwen Zhang, Dan Li, Bohong Cen, Wanwen Cao, Zhongyuan Xu","doi":"10.1002/bmc.70108","DOIUrl":"https://doi.org/10.1002/bmc.70108","url":null,"abstract":"<div>\u0000 \u0000 <p>Dapagliflozin is a widely used sodium-glucose cotransporter 2 inhibitor that has been approved for the treatment of Type 2 diabetes. In this study, we present a simple and sensitive high-performance liquid chromatography tandem mass spectrometry (HPLC–MS/MS) method for the simultaneous quantification of dapagliflozin and its metabolite, dapagliflozin 3-O-glucuronide (D3OG). The experiments were conducted using an Agilent G6495B triple quadrupole mass spectrometer coupled with an Agilent 1290 Infinity II HPLC system, featuring a Poroshell 120 EC-C18 column. Gradient elution was performed with ammonium formate (10 mM) and methanol as the mobile phase. The G6495B was operated in negative ion mode with electrospray ionization and multiple reaction monitoring. The quantitative method was validated according to FDA and EMA guidelines, assessing parameters such as selectivity, linearity, accuracy, precision, dilution integrity, stability, and recovery. Methanol was used as a protein precipitant during sample preparation, resulting in consistent extraction recoveries ranging from 91% to 96% for all analytes. The linear range for the analytes was established at 2–800 μg/L with a sample volume of 100 μL. This validated method is sufficient for the simultaneous quantification of dapagliflozin and D3OG in plasma and has been successfully applied in pharmacokinetic studies, bioequivalence assessments, and clinical therapeutic monitoring.</p>\u0000 <p><b>Trial Registration:</b> Chinese Clinical Trial identifier: ChiCTR2100044600</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143919364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0