Biomedical Chromatography最新文献_第2页

A Novel Polarity Switching LC-MS/MS Method for Simultaneous Quantification of Drospirenone and Estetrol: Application to Pharmacokinetic Studies in Human Plasma 一种新型极性开关LC-MS/MS同时定量drosprenone和esteol的方法：在人血浆药代动力学研究中的应用。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-10-02 DOI: 10.1002/bmc.70633

Pravesh Singh, Sajad Khaliq Dar, Faisal Hamid Khan, Asjad Nabi Khan Sherwani, Sanjay Jagannath Gurule, Onkar Singh, Sudershan Kumar, Arshad H. Khuroo

{"title":"A Novel Polarity Switching LC-MS/MS Method for Simultaneous Quantification of Drospirenone and Estetrol: Application to Pharmacokinetic Studies in Human Plasma","authors":"Pravesh Singh, Sajad Khaliq Dar, Faisal Hamid Khan, Asjad Nabi Khan Sherwani, Sanjay Jagannath Gurule, Onkar Singh, Sudershan Kumar, Arshad H. Khuroo","doi":"10.1002/bmc.70633","DOIUrl":"10.1002/bmc.70633","url":null,"abstract":"<div>\u0000 \u0000 A sensitive, selective, and rapid liquid chromatography–tandem mass spectrometry (LC-MS/MS) method using a polarity switch technique was developed and validated for the simultaneous determination of drospirenone (DSP) and estetrol (E4) in human plasma for the first time. Solid phase extraction (SPE) with Bond Elut PLEXA cartridges was performed. Chromatographic separation was obtained on Eclipse XDB-C8 (150 mm × 4.6 mm, 3.5 μm) column using 2-mM ammonium bicarbonate solution:methanol (30:70, v/v) as the mobile phase at a flow rate of 0.750 mL/min. A polarity switching method was performed in a triple quadrupole mass spectrometer using multiple reaction monitoring (MRM). MRM was performed in positive mode for DSP transitions m/z 367.2 → 171.1 and DSP-D4 transitions m/z 371.2 → 171.1, and in negative mode for E4 transitions m/z 303.1 → 241.2, 273.0 and E4-d3 transitions m/z 306.2 → 243.2, 275.0. Calibration curves were linear over the concentration ranges of 0.351–100.446 and 0.351–50.134 ng/mL for DSP and E4, respectively. The accuracy measured for both analytes ranged from −2.74% to 4.21%. Both the analytes showed consistent recoveries ranged from 77.25% to 81.34% with no significant matrix effect. The method was successfully applied in a pharmacokinetic study of DSP and E4 tablets (3 mg + 14.2 mg).\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 11","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A One-Min, FDA-Validated UPLC–MS/MS Method for the AML Smoothened Inhibitor Glasdegib Quantification and Metabolic Stability Assessment in Human Liver Microsomes: In Silico Metabolic Lability and Toxicity Screening 用于AML平滑抑制剂Glasdegib定量和人肝微粒体代谢稳定性评估的1分钟、fda验证的UPLC-MS/MS方法：计算机代谢不稳定性和毒性筛选

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-10-01 DOI: 10.1002/bmc.70631

Mohamed W. Attwa, Haitham AlRabiah, Ahmed H. Bakheit, Adnan A. Kadi

{"title":"A One-Min, FDA-Validated UPLC–MS/MS Method for the AML Smoothened Inhibitor Glasdegib Quantification and Metabolic Stability Assessment in Human Liver Microsomes: In Silico Metabolic Lability and Toxicity Screening","authors":"Mohamed W. Attwa, Haitham AlRabiah, Ahmed H. Bakheit, Adnan A. Kadi","doi":"10.1002/bmc.70631","DOIUrl":"10.1002/bmc.70631","url":null,"abstract":"<div>\u0000 \u0000 Glasdegib (GSB) is an orally bioavailable Smoothened (Hedgehog pathway) inhibitor approved, in combination with low-dose cytarabine, for the treatment of newly diagnosed acute myeloid leukemia (AML) in patients who are unfit for intensive induction chemotherapy. Although GSB is predominantly cleared by hepatic CYP3A4-mediated oxidation, no human liver microsomes (HLM) metabolic stability method has been reported; existing assays are limited to plasma matrices. Here, we describe an ultra-fast validated UPLC–MS/MS method for GSB and apply it to the first assessment of metabolic stability in HLM. StarDrop WhichP450 (CSL = 0.8928) and DEREK alerts were performed to localize metabolic soft spots and safety liabilities. Chromatography was performed on an SB-C18 column in 1 min. The method was linear over 1–4000 ng/mL (y = 1.2753x + 0.4567; r2 = 0.9992), with accuracy (−3.10 to +10.25 %E) and precision (≤ 3.56 %RSD). GSB exhibited an in vitro t1/2 of 44.15 min and an intrinsic clearance of 18.37 mL/min/kg (moderate-clearance compound). The predicted soft spots (the N-methylpiperidine ring [65%] and the benzimidazole moiety [29%]) are consistent with the known human N-desmethyl metabolite and CYP3A4 dominance. The combined dataset provides a soft-spot-guided rationale for the metabolic optimization of future Hedgehog-pathway inhibitors.\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 11","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the Chemical Constituents in Shenyanshu Tablets by Ultra-High-Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry 超高效液相色谱-四极杆飞行时间质谱法表征肾炎舒片中化学成分。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-09-30 DOI: 10.1002/bmc.70606

Xing Wang, Jiao Guan, Chunfu Zhang, Yanwu Guan, Lihe Wang, Yan Yang, Shuangyu Li, Yang Liu, Lele Li, Yue Cui, Heyun Zhu

{"title":"Characterization of the Chemical Constituents in Shenyanshu Tablets by Ultra-High-Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry","authors":"Xing Wang, Jiao Guan, Chunfu Zhang, Yanwu Guan, Lihe Wang, Yan Yang, Shuangyu Li, Yang Liu, Lele Li, Yue Cui, Heyun Zhu","doi":"10.1002/bmc.70606","DOIUrl":"10.1002/bmc.70606","url":null,"abstract":"<div>\u0000 \u0000 Shenyanshu tablets (SYST), a widely prescribed traditional Chinese patent medicine, which is clinically applied to treat chronic glomerulonephritis in China and other Asian countries. However, there is still no detailed report existing on its chemical composition at present, which hinders further investigation into its pharmacological activities and mechanism of action. To solve this difficult problem, an ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry method was established to separate and identify the chemical constituents of SYST. A total of 130 compounds were identified or characterized, among which 19 compounds were clearly confirmed by comparing their retention time and mass spectrometry data with those of reference standards. Based on the mass spectrometry data and relevant literature, the remaining compounds were preliminarily characterized. Results revealed that flavonoids, triterpenoid glycosides, organic acids, and alkaloids were the primary chemical components of SYST. The established analytical method has been proven to effectively characterize the chemical constituents of SYST and also provides important reference for clarifying the pharmacodynamic material basis and mechanism of action of SYST.\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 11","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical Profiling, Antioxidant Activity and Cytotoxic Potential of Chloroxylon swietenia DC. Stem Extracts Against Bladder Cancer Cell Lines 甜叶兰的植物化学特征、抗氧化活性和细胞毒潜能。干细胞提取物对膀胱癌细胞系的抑制作用

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-09-29 DOI: 10.1002/bmc.70616

Umashri Jambale, Shivasharana Chandrabanda Thimmappa

{"title":"Phytochemical Profiling, Antioxidant Activity and Cytotoxic Potential of Chloroxylon swietenia DC. Stem Extracts Against Bladder Cancer Cell Lines","authors":"Umashri Jambale, Shivasharana Chandrabanda Thimmappa","doi":"10.1002/bmc.70616","DOIUrl":"10.1002/bmc.70616","url":null,"abstract":"<div>\u0000 \u0000 Chloroxylon swietenia DC. is traditionally used in herbal medicine and is known to contain various secondary metabolites with pharmacological activities. This study includes qualitative and quantitative analysis of phytochemicals, antioxidant and anticancer potential of C. swietenia stem extracts. Stem extracts were prepared with chloroform, acetone, methanol and water. Phytochemical analysis revealed secondary metabolites, extracts were further analysed for total phenolic and flavonoid content, and bioactive compounds were identified by GC–MS analysis. Antioxidant activity was tested using total antioxidant capacity, DPPH and FRAP assays. Cytotoxicity was assessed on T24 cells using MTT. Methanol extract revealed the presence of flavonoids, glycosides, phenolics, saponins and tannins; acetone extract revealed the same presence of same secondary metabolites except saponins, leaving methanol extract with the highest diversity. Acetone had higher TPC (90.876 ± 0.964 mgGAE/g), whereas methanol had higher TFC (122.115 ± 0.962 mgQE/g). GC–MS identified the highest compounds in acetone. The highest total antioxidant capacity was 1.33 ± 0.02 (acetone). The methanol extract exhibited significant DPPH-scavenging activity with an IC50 of 119.080 μg/mL. Ferric reducing power was highest for methanol (0.75 ± 0.01). Acetone was most cytotoxic to T24 cells (IC50 17.131 μg/mL), followed by chloroform, aqueous and methanol. This study finds the presence of bioactive compounds in C. swietenia stem, which are potential natural sources with antioxidant and anticancer properties for further research.\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 11","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149073416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of Amlexanox in Beagle Dog Plasma by LC–MS/MS and Its Application in a Comparative Pharmacokinetic Study of Conventional and Sustained-Release Tablets LC-MS/MS法测定比格犬血浆中氨lexanox含量及其在常规与缓释片药动学比较研究中的应用

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-09-25 DOI: 10.1002/bmc.70625

Yanmei Yi, Wei He, Xiaochen Niu, Jianchun Zhao

{"title":"Quantification of Amlexanox in Beagle Dog Plasma by LC–MS/MS and Its Application in a Comparative Pharmacokinetic Study of Conventional and Sustained-Release Tablets","authors":"Yanmei Yi, Wei He, Xiaochen Niu, Jianchun Zhao","doi":"10.1002/bmc.70625","DOIUrl":"10.1002/bmc.70625","url":null,"abstract":"<div>\u0000 \u0000 Amlexanox, a 5H-benzopyranopyridine derivative with emerging therapeutic potential in metabolic and inflammatory diseases, lacks a validated analytical method for pharmacokinetic studies in beagle dogs. A sensitive LC–MS/MS method was developed and validated for amlexanox in beagle dog plasma according to ICH M10 guidelines. Propranolol served as internal standard. Samples were processed by protein precipitation, and separation was achieved on a CAPCELL PAK C18 column using gradient elution with water and acetonitrile (both containing 0.2% formic acid) at 400 μL/min. Detection used positive electrospray ionization with MRM of m/z 299.0 → 281.0 for amlexanox and m/z 260.0 → 116.1 for IS. The method showed good linearity over 40–2000 ng/mL (R2 > 0.99). All validation parameters met acceptance criteria. The method was applied to a three-period fixed-sequence study in beagle dogs (n = 3, male) comparing intravenous injection (1 mg/kg), conventional tablets (75 mg), and sustained-release tablets (150 mg). The sustained-release formulation prolonged Tmax (3.67 vs. 1.00 h) and residence time (two-fold increase in t1/2 and MRT), and improved absolute bioavailability from 25.36% to 33.09% (relative bioavailability: 151.30%). These findings support the clinical development of a sustained-release amlexanox formulation with reduced dosing frequency and improved patient compliance.\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 11","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Metabolic Profiling of Brucea javanica Oil Oral Emulsion in Rat Serum by Ultrahigh Performance Liquid Chromatography Time-of-Flight Mass Spectrometry and Its Correlation With Anti-Glioblastoma Activity 超高效液相色谱-飞行时间-质谱法分析鸦胆子油口服乳剂在大鼠血清中的综合代谢特征及其与抗胶质母细胞瘤活性的相关性

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-09-25 DOI: 10.1002/bmc.70628

Guangliang Zhang, Wentao Shao, Junbin Shu, Jianfeng Tong, Jing Zhang, Yi Tao

{"title":"Comprehensive Metabolic Profiling of Brucea javanica Oil Oral Emulsion in Rat Serum by Ultrahigh Performance Liquid Chromatography Time-of-Flight Mass Spectrometry and Its Correlation With Anti-Glioblastoma Activity","authors":"Guangliang Zhang, Wentao Shao, Junbin Shu, Jianfeng Tong, Jing Zhang, Yi Tao","doi":"10.1002/bmc.70628","DOIUrl":"https://doi.org/10.1002/bmc.70628","url":null,"abstract":"<div>\u0000 \u0000 Brucea javanica oil oral emulsion (BJOE) serves as an adjunctive therapeutic agent for glioblastoma multiforme (GBM); however, the inhibitory effects of its serum metabolites remain unexplored. In this study, ultrahigh performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS) was employed to analyze serum metabolites in rats following BJOE administration. The inhibitory effects of the serum on GBM were assessed using U251 cell bioassays, and the relationship between serum metabolites and cellular inhibitory activity was examined. Six prototype components and six metabolites were identified in the serum, including linoleic acid, linoleic acid-M1, linoleic acid-M2, linoleic acid-M3, oleic acid, oleic acid-M1, oleic acid-M2, oleic acid-M3, sphingosine, palmitoleic acid, oleamide, and 1-stearoyl-rac-glycerol. Rat serum collected 2 h postdosing significantly inhibited U251 cell proliferation. Gray relationship analysis indicated that oleic acid, oleic acid-M1, linoleic acid, linoleic acid-M1, linoleic acid-M2, sphingosine, palmitoleic acid, oleamide, and 1-stearoyl-rac-glycerol were highly correlated with the inhibition rate of U251 cell proliferation, with oleic acid-M1 showing the highest correlation coefficient (0.9806). These findings suggest that BJOE and its metabolites may possess therapeutic potential against GBM, particularly in inhibiting glioma cell proliferation, with oleic acid and its metabolites likely playing a significant role.\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 11","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149048447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UHPLC–HRMS-Based Tissue Pharmacochemistry and Metabolomics Reveal the Effective Components and Potential Mechanism of Huoxue Huayu Gel Plaster Against Oxaliplatin-Induced Peripheral Neuropathy 基于uhplc - hrms的组织药物化学和代谢组学揭示活血化瘀凝胶膏抗奥沙利铂诱导的周围神经病变的有效成分和潜在机制。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-09-24 DOI: 10.1002/bmc.70626

Yu-jiao Hua, Yan Zhang, Yong-juan Ding, Jing-hua Chen

{"title":"UHPLC–HRMS-Based Tissue Pharmacochemistry and Metabolomics Reveal the Effective Components and Potential Mechanism of Huoxue Huayu Gel Plaster Against Oxaliplatin-Induced Peripheral Neuropathy","authors":"Yu-jiao Hua, Yan Zhang, Yong-juan Ding, Jing-hua Chen","doi":"10.1002/bmc.70626","DOIUrl":"10.1002/bmc.70626","url":null,"abstract":"<div>\u0000 \u0000 Huoxue Huayu Gel Plaster (HXHYGP) is a topical traditional Chinese medicine preparation used for oxaliplatin-induced peripheral neuropathy (OIPN), but its locally exposed bioactive components remain unclear. This study developed an integrated strategy combining pharmacodynamic evaluation, UHPLC-HRMS-based tissue pharmacochemistry, untargeted metabolomics, network pharmacology, molecular docking, and qRT-PCR to identify effective components and explore potential mechanisms. In OIPN rats, HXHYGP improved mechanical and cold sensory dysfunction; reduced plantar tissue injury and inflammatory-cell infiltration; increased nerve growth factor levels; decreased TNF-α, IL-6, and IL-1β levels; and enhanced nerve conduction velocity. A total of 496 constituents were detected in skin tissue, including 20 tissue-migrant constituents attributed to HXHYGP. Untargeted metabolomics identified 53 OIPN-related differential metabolites, of which 42 were significantly reversed after treatment. Correlation analysis and network pharmacology further identified 14 effective components and 98 common targets, with EGFR, PIK3CA, MTOR, RELA, and MAPK14 as key nodes. Molecular docking supported potential component–target interactions, while qRT-PCR confirmed reduced mRNA expression of these targets after HXHYGP treatment. These findings indicate that HXHYGP may alleviate OIPN by modulating the proposed EGFR/PI3K/AKT/mTOR/NF-κB signaling network and MAPK14-associated inflammation. This strategy provides an analytical basis for identifying pharmacodynamic substances and potential quality-marker candidates in topical traditional Chinese medicine preparations.\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 11","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Online HPLC–DPPH-Guided Isolation and Free Radical-Scavenging Evaluation of C-4″-Epimeric Quercetin Diglycosides From Saxifraga tangutica 高效液相色谱- dpph在线分离及体外清除自由基评价藏红花C-4″-表皮槲皮素二糖苷。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-09-24 DOI: 10.1002/bmc.70627

Chuang Liu, Yurong Du, Jianbin Ma, Minxia Zhu, Jun Dang

{"title":"Online HPLC–DPPH-Guided Isolation and Free Radical-Scavenging Evaluation of C-4″-Epimeric Quercetin Diglycosides From Saxifraga tangutica","authors":"Chuang Liu, Yurong Du, Jianbin Ma, Minxia Zhu, Jun Dang","doi":"10.1002/bmc.70627","DOIUrl":"10.1002/bmc.70627","url":null,"abstract":"<div>\u0000 \u0000 Activity-guided separation of medicinal plants requires linking activity signals and chromatographic resolution in complex matrices. In this study, based on a previously established HPLC–DPPH platform, medium-pressure prefractionation and preparative chromatographic purification were applied for the targeted isolation of free radical scavengers from Saxifraga tangutica (S. tangutica). Medium-pressure prefractionation reduced sample complexity and enriched active fractions, while Click XION chromatography concentrated the target fraction. Subsequent purification on the ReproSil-Pur C18 AQ column resolved this fraction into two baseline-separated active peaks, affording compounds with purities greater than 95%. The isolated compounds were identified as quercetin-3-O-β-D-glucoside-7-O-β-D-glucoside and quercetin-3-O-β-D-galactoside-7-O-β-D-glucoside, a pair of C-4″-epimeric quercetin 3,7-di-O-glycosides. Both compounds exhibited DPPH radical-scavenging activity, with IC50 values of 68.94 ± 2.77 and 75.59 ± 2.14 μM, respectively. DFT calculations, molecular docking, and ADMET prediction further supported the comparative interpretation of their electronic features, antioxidant-related interaction potential, and predicted pharmacokinetic profiles. Overall, this study achieved the activity guided isolation and activity evaluation of a pair of high-purity C-4″-epimeric flavonol diglycosides from S. tangutica, providing a practical chromatographic strategy for the targeted characterization of structurally similar bioactive constituents from medicinal plants.\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 11","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UHPLC-MS/MS–Based Simultaneous Quantification and Pharmacokinetic Characterization of 20 Components of Xiangju Rupining Capsule in Rats 香菊乳宁胶囊20种成分在大鼠体内的同时定量及药动学表征。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-09-21 DOI: 10.1002/bmc.70624

Ru Cang, Xue Meng, Jin Li, Kunze Du, Yanxu Chang

{"title":"UHPLC-MS/MS–Based Simultaneous Quantification and Pharmacokinetic Characterization of 20 Components of Xiangju Rupining Capsule in Rats","authors":"Ru Cang, Xue Meng, Jin Li, Kunze Du, Yanxu Chang","doi":"10.1002/bmc.70624","DOIUrl":"10.1002/bmc.70624","url":null,"abstract":"<div>\u0000 \u0000 Xiangju Rupining Capsule (XJRPN) is a Chinese patent medicine clinically used for the treatment of mammary gland hyperplasia. The in vivo pharmacokinetic profiles of its components remain largely unexplored, thereby constraining the elucidation of its pharmacodynamic material basis. Therefore, a rapid and sensitive ultra high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) assay was developed and fully validated for the simultaneous quantification of 20 major prototype components in rat plasma following oral administration of XJRPN. The lower limit of quantification (LLOQ) for all 20 target analytes ranged from 0.02 to 2.5 ng/mL. All components exhibited satisfactory linearity (R2 ≥ 0.9940), and the intraday and interday accuracy, precision, and stability conformed to the standard acceptance criteria for bioanalytical method validation. Systemic exposure (Cmax and AUC) varied substantially across different chemical classes. Application of this method revealed diverse pharmacokinetic behaviors among the 20 constituents, providing the first systematic characterization of the in vivo disposition of XJRPN prototype components in rats. This study establishes an essential foundation for clarifying the material basis underlying XJRPN's pharmacological effects and informs strategies for its rational clinical application.\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 11","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149011830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Quantification of Eight Bioactive Markers Coupled With HPLC Fingerprint and Chemometric Analysis for Quality Discrimination of Saposhnikovia divaricata From Diverse Geographical Origins 8种生物活性标记同时定量结合HPLC指纹图谱和化学计量学分析在不同产地三叶参质量鉴别中的应用

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-09-19 DOI: 10.1002/bmc.70623

Wenping Liu, Meng Liu, Wenlong Zhao, Jin Sun, Xiaofei Wu, Keming Li, Liuyang Fan

{"title":"Simultaneous Quantification of Eight Bioactive Markers Coupled With HPLC Fingerprint and Chemometric Analysis for Quality Discrimination of Saposhnikovia divaricata From Diverse Geographical Origins","authors":"Wenping Liu, Meng Liu, Wenlong Zhao, Jin Sun, Xiaofei Wu, Keming Li, Liuyang Fan","doi":"10.1002/bmc.70623","DOIUrl":"https://doi.org/10.1002/bmc.70623","url":null,"abstract":"<div>\u0000 \u0000 Saposhnikovia divaricata (SD, Chinese name: Fangfeng) is a commonly used herbal medicine in traditional Chinese medicine (TCM), yet unclear chemical profiling and remarkable quality heterogeneity among commercial batches restrict its standardized quality supervision. This study established an integrated strategy combining high-performance liquid chromatography (HPLC) fingerprinting and multivariate chemometrics to quantify bioactive compounds and discriminate geographical origins of SD. Eight bioactive markers including four chromones and four coumarins were unambiguously assigned among 18 shared characteristic fingerprint peaks. Obvious disparities in target compound contents were observed between geographically distinct samples. Fingerprint similarity results confirmed overall chemical consistency across all batches, accompanied by intra-region quality fluctuation. Geographical habitats exerted prominent effects on the accumulation of eight target constituents in SD. Combined hierarchical cluster analysis (HCA) and partial least squares discriminant analysis (PLS-DA) models successfully separated batches from different habitats, and three differential markers (prim-O-glucosylcimifugin, cimifugin, and imperatorin) were screened to interpret regional chemical divergence. In conclusion, this study provides an experimental foundation for drug quality control and the discovery of quality markers for SD. Imperatorin can be supplemented to construct a multi-index comprehensive quality evaluation system for SD.\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 11","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148994638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0