Biomedical Chromatography最新文献_第2页

Development and Validation of a HPLC-MS/MS Method for the Determination of Compound 13b in Rat Plasma and Its Application to a Pharmacokinetic Study

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-12 DOI: 10.1002/bmc.70044

Guofang Bi, Qingqing Yu, Jiayin Guo, Shuqing Zhang, Peng Wang, Xiaowen Jiang, Chenghua Wu, Shuang Hu, Xiao Yang, Jian-Hong Fang, Yuhua Long, Huichang Bi

{"title":"Development and Validation of a HPLC-MS/MS Method for the Determination of Compound 13b in Rat Plasma and Its Application to a Pharmacokinetic Study","authors":"Guofang Bi, Qingqing Yu, Jiayin Guo, Shuqing Zhang, Peng Wang, Xiaowen Jiang, Chenghua Wu, Shuang Hu, Xiao Yang, Jian-Hong Fang, Yuhua Long, Huichang Bi","doi":"10.1002/bmc.70044","DOIUrl":"https://doi.org/10.1002/bmc.70044","url":null,"abstract":"<div>\u0000 \u0000 <p>Compound 13b, a newly identified anthraquinone derivative, has demonstrated potent efficacy against the Zika virus. To explore the bioavailability and pharmacokinetic properties of compound 13b, a robust and sensitive HPLC-MS/MS method was established and verified to determine its plasma concentration in rats. Sample preparation involved protein precipitation using acetonitrile with testosterone as an internal standard. A Phenomenex Kinetex XB-C18 column (2.1 × 100 mm, 2.6 μm) was utilized for sample separation with gradient elution. The mobile phase, consisting of water and acetonitrile, was dispensed at a flow rate of 1 mL/min. The multiple reaction monitoring mode (MRM) approach was used to detect compound 13b and testosterone, characterized by their respective ionization transitions: m/z 410.2 → 91.1 and m/z 289.2 → 109.2. The method demonstrated superior linearity within rat plasma samples, ranging from 2 to 400 ng/mL, and met all FDA guidelines for bioanalytical method validation. The pharmacokinetic properties were calculated by non-compartmental approach following administration of compound 13b at 14 mg/kg in rats, and the absolute oral bioavailability was found to be 15.45%. Hence, a highly sensitive and rapid HPLC-MS/MS method for measuring plasma concentration of compound 13b in rats has been successfully developed, rigorously validated, and subsequently utilized in a bioavailability and pharmacokinetic study.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution and Pharmacokinetic Characteristics of Cordycepin in Rat: Investigated by UHPLC-HRMS/MS and Blood–Brain Synchronous Microdialysis

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-12 DOI: 10.1002/bmc.70038

Mengjiao Li, Fuqiang Liu, Lulu Guo, Wu Fan, Jiazhong Wang, Binbin Lu, Guangfeng Hong, Wenjuan Zhang, Shu Tian, Jian Mao, Jianping Xie

{"title":"Distribution and Pharmacokinetic Characteristics of Cordycepin in Rat: Investigated by UHPLC-HRMS/MS and Blood–Brain Synchronous Microdialysis","authors":"Mengjiao Li, Fuqiang Liu, Lulu Guo, Wu Fan, Jiazhong Wang, Binbin Lu, Guangfeng Hong, Wenjuan Zhang, Shu Tian, Jian Mao, Jianping Xie","doi":"10.1002/bmc.70038","DOIUrl":"https://doi.org/10.1002/bmc.70038","url":null,"abstract":"<div>\u0000 \u0000 <p>Cordycepin, a natural adenosine derivative, exhibits multiple pharmacological effects on organisms. However, its distribution and metabolic characteristics have not been fully elucidated in vivo. In this study, ultra-high liquid chromatography tandem high-resolution mass spectrometry (UHPLC-HRMS/MS) was used to investigate the pharmacokinetic characteristics and effects of cordycepin on endogenous adenosine and inosine. Microdialysis was used for real-time monitoring of unbound drug in brain and blood, whereas conventional tissue homogenate methods assessed distribution in various tissues. Results showed that the distribution pattern of cordycepin was as follows: kidney > liver > heart > lung > spleen > brain. Cordycepin administration significantly altered the levels of adenosine and inosine in heart and liver. Synchronous microdialysis sampling for the pharmacokinetic profile indicated that cordycepin was rapidly consumed and 3′-deoxyinosine was generated as the main metabolite. The <i>C</i><sub>max</sub> values of cordycepin in the rat blood and brain after exposure (10 mg/kg, i.p.) were 7.8 and 5.4 ng/mL, respectively. Mean residence time in blood and brain was 102.2 and 137.0 min, respectively. Inhibition of adenosine deaminase by racemic 9-(2-hydroxy-3-nonyl) adenine hydrochloride (EHNA) enhanced cordycepin levels in the blood. This work provides a solid basis for understanding the metabolism of cordycepin and its pharmacological effects.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comprehensive Analysis of Market Forms and Analytical Methods for Metformin Formulations

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-12 DOI: 10.1002/bmc.70028

Saravana Muthu Kumar Pandian, Punniyakoti V. Thanikachalam, Deviga Kaliyappan, Anandarajagopal Kalusalingam

{"title":"A Comprehensive Analysis of Market Forms and Analytical Methods for Metformin Formulations","authors":"Saravana Muthu Kumar Pandian, Punniyakoti V. Thanikachalam, Deviga Kaliyappan, Anandarajagopal Kalusalingam","doi":"10.1002/bmc.70028","DOIUrl":"https://doi.org/10.1002/bmc.70028","url":null,"abstract":"<div>\u0000 \u0000 <p>Metformin has been a cornerstone in the management of type 2 diabetes mellitus (T2DM) for more than 50 years, either alone or in combination with other therapies. This oral antihyperglycemic agent, also known as dimethylbiguanide, plays a crucial role in regulating noninsulin-dependent diabetes mellitus and is widely prescribed globally for various medical conditions. Recent advancements in its formulations have aimed to increase its effectiveness, tolerance, and nonglycemic effects. This review critically evaluates the analytical methods used to assess metformin formulations, including chromatographic and spectroscopic techniques, with a focus on sensitivity, specificity, and reliability. Comprehensive literature from various scientific databases was searched to gather information on metformin. Various formulations of metformin HCl, including hydrogels, solid dosage forms, mucoadhesive patches, lipospheres, and topical preparations, offer advantages like sustained release, improved bioavailability, and wound-healing potential. Analytical methods like RP-HPLC and UV–visible spectroscopy ensure the safety, stability, and accurate quantification of these formulations. These approaches support personalized treatment of T2DM, enhancing blood sugar control, therapeutic efficacy, and patient outcomes. This review offers valuable insights into metformin formulations and analytical aspects that researchers and pharmaceutical experts benefit in the further development and evaluation of metformin formulations.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effects and Mechanisms of Active Fractions of Ixeris sonchifolia on Rats With Toxic Heat and Blood Stasis Syndrome Revealed by Pharmacodynamics and Metabonomics

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-12 DOI: 10.1002/bmc.70042

Zhaowei Liu, Yuanyuan Zhao, Yiran Ren, Yifei Liu, Zhenqing Liu, Jingxuan Zhang, Ying Liu

{"title":"Protective Effects and Mechanisms of Active Fractions of Ixeris sonchifolia on Rats With Toxic Heat and Blood Stasis Syndrome Revealed by Pharmacodynamics and Metabonomics","authors":"Zhaowei Liu, Yuanyuan Zhao, Yiran Ren, Yifei Liu, Zhenqing Liu, Jingxuan Zhang, Ying Liu","doi":"10.1002/bmc.70042","DOIUrl":"https://doi.org/10.1002/bmc.70042","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Ixeris sonchifolia</i> (IS) has been demonstrated to have beneficial effects on clearing heat and detoxifying, promoting blood circulation and removing blood stasis. However, the protective effects of active fractions and the underlying mechanisms of IS against toxic heat and blood stasis syndrome (THBSS) remain unclear. This study aimed to investigate this. In this study, the protective effects of 30%, 50%, and 70% ethanol fractions of IS on rats with THBSS were evaluated by observing myocardial pathology, analyzing blood rheology and blood routine indices, and the general conditions of the rats. Comprehensive metabolomic analysis of plasma and myocardium samples was conducted using ultra-high-performance liquid chromatography linear ion trap quadrupole orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap MS/MS). The results showed that IS-30% had a significant protective effect on rats with THBSS, and it could play a protective role by modulating 35 differential metabolites associated with key metabolic pathways, including glycerophospholipid metabolism, sphingosine metabolism, alpha-linolenic acid metabolism, and nicotinate and nicotinamide metabolism. Analysis of different metabolites and pathways showed that they are closely associated with inflammatory responses; therefore, it is hypothesized that IS-30% may protect against THBSS by exerting an anti-inflammatory effect. This study provides valuable data supporting the druggability of IS-30% and offers new insights into its potential therapeutic applications.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stability-Indicating RP-HPLC Method Development and Validation for Determination of Impurities in Loperamide Hydrochloride Capsules Dosage Form

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-12 DOI: 10.1002/bmc.70027

Sreenivas Pippalla, Vaishnavi Chintala

{"title":"Stability-Indicating RP-HPLC Method Development and Validation for Determination of Impurities in Loperamide Hydrochloride Capsules Dosage Form","authors":"Sreenivas Pippalla, Vaishnavi Chintala","doi":"10.1002/bmc.70027","DOIUrl":"https://doi.org/10.1002/bmc.70027","url":null,"abstract":"<div>\u0000 \u0000 <p>A quality by design (QbD)-based high-resolution, stability-indicating high-performance liquid chromatography (HPLC) method was developed for determining impurities in loperamide hydrochloride (LPH) tablet dosage forms. Using this method, eight known impurities were qualified, and three degradants were quantified with excellent peak resolution. Mobile Phase-A consisted of 0.05-M tetrabutylammonium hydrogen phosphate buffer and acetonitrile (80:20, v/v), while Mobile Phase-B contained the same buffer and acetonitrile (20:80, v/v). The gradient program was as follows: 0 min, MP-A 95%, MP-B 5%; 15 min, MP-A 30%, MP-B 70%; 17 min, MP-A 30%, MP-B 70%; 19 min, MP-A 95%, MP-B 5%; and 24 min, MP-A 95%, MP-B 5%. Chromatographic separation was performed using a column zodiac C18 (100 mm × 4.6 mm, 3.0 μm) with a flow rate of 1.5 mL/min, detection at 220 nm, an injection volume of 10 μL, and a column temperature of 35°C.</p>\u0000 <p>Stress studies revealed LPH's sensitivity to acidic, oxidative, and thermal conditions. Under all forced degradation conditions, the purity angle was found to be less than the purity threshold, demonstrating the robustness of the method. Validation confirmed its suitability for quality analysis and stability studies in routine manufacturing processes.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on Rapid Detection Technology of Illegal Chemical Compounds Added to External Poultices

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-10 DOI: 10.1002/bmc.70053

Xiaoan Li, Yaning Cui, Li Liu, Longjiang Guo, Yudong Liu, Xuegao Song, Na Song, Jianzhong Jia, Zhuanping Zhang

{"title":"Study on Rapid Detection Technology of Illegal Chemical Compounds Added to External Poultices","authors":"Xiaoan Li, Yaning Cui, Li Liu, Longjiang Guo, Yudong Liu, Xuegao Song, Na Song, Jianzhong Jia, Zhuanping Zhang","doi":"10.1002/bmc.70053","DOIUrl":"https://doi.org/10.1002/bmc.70053","url":null,"abstract":"<div>\u0000 \u0000 <p>Topical poultices and applied medical devices that can relieve a variety of cervical vertebrae and lumbar vertebrae pain may have illegal additives of pharmaceutical drugs or analogues have additional health risks, but customers may not be aware of what they are using. National Medical Products Administration reviewed and approved “Supplementary test method for identification and content determination of 17 chemicals in affixed medical devices” drafted by Shandong Provincial Institute of Medical Devices and Drug Packaging Inspection in January 2022, but the test method is simple in sample processing and difficult to extract the chemical components in the paste that may cause the phenomenon of missing detection of most illegal added chemical drugs. In this work, the sample handling and extraction methods have been improved and optimized, the content of illegal added chemicals in 47 batches of affixed medical devices was determined by HPLC-MS/MS method and was detected in 37 batches, and the non-steroidal anti-inflammatory drug nimesulide and aspirin were detected in most samples. It shows that there are illegal chemical drugs added in the medical devices and many people may take several different supplements daily and encounter quite high levels of combined exposure to toxic compounds.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pioneering Human Plasma Detection of Haloperidol With 5-pg/mL Sensitivity via Refined Sample Preparation and Advanced LC–MS/MS

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-10 DOI: 10.1002/bmc.70048

Santosh Tawari, Ujashkumar Shah

{"title":"Pioneering Human Plasma Detection of Haloperidol With 5-pg/mL Sensitivity via Refined Sample Preparation and Advanced LC–MS/MS","authors":"Santosh Tawari, Ujashkumar Shah","doi":"10.1002/bmc.70048","DOIUrl":"https://doi.org/10.1002/bmc.70048","url":null,"abstract":"<div>\u0000 \u0000 <p>The present study develops, refines, and validates an LC–MS/MS technique to detect haloperidol in human plasma with outstanding sensitivity (5 pg/mL), selectivity, specificity, fast analysis time, and low sample volume to enhance mental treatment regimens. Haloperidol and haloperidol D4 were tested on a Kromasil C18 stationary phase with a 35:65 ratio of 10-mM ammonium trifluoroacetate buffer to acetonitrile. A Strata-X PRO cartridge extracted the analyte and IS with improved sample extraction. ESI with multiple reaction monitoring measured haloperidol (Q1/Q3: 376.1/165.0) and D4 (Q1/Q3: 380.1/169.0). This method has high sensitivity (5.03 pg/mL), extraction efficiency (> 95%), rapid analysis (3 min), and a small sample volume (100 μL). The correction coefficient (<i>r</i><sup>2</sup>) > 0.980 linearized the process from 5.03 to 6020.75 pg/mL. Nominal accuracy was 95.40%–102.52%, while intraday precision (% CV) was 0.92%–5.33%. Additionally, interday accuracy ranged from 95.40% to 102.66% and precision from 2.05% to 5.73%. This bioanalytical method for haloperidol detection in human plasma shows great sensitivity, selectivity, and linearity with an LLOQ of 5.03 pg/mL. It improves pharmacokinetics, bioequivalence, and scale-up bioavailability. Being precise, stable, and adaptable are all advantages in clinical and therapeutic monitoring.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Plasma Protein Binding Determination of Lefamulin by Equilibrium Dialysis and High-Performance Liquid Chromatography Coupled With Evaporative Light Scattering Detector

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-10 DOI: 10.1002/bmc.70052

Lin Xin, Hong-Liang Xiao, Mei-Di Li, Yi-Kuan Ji, Wen-Zi Liu, Xiao-Ping Liao, Yang Yu, Dong-Hao Zhao

引用次数: 0

A Novel Derivatization-Free Approach for Analysis of (+)2-Aminobutanol and Ethambutol Hydrochloride in Tablets Preparation Using RP-HPLC-NQAD

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-10 DOI: 10.1002/bmc.70049

Hao Ruan, Ying Luo, Qian Yang, Chao Liu, Yunfeng Shi, Minglong Ye, Liya Hong, Weike Su

{"title":"A Novel Derivatization-Free Approach for Analysis of (+)2-Aminobutanol and Ethambutol Hydrochloride in Tablets Preparation Using RP-HPLC-NQAD","authors":"Hao Ruan, Ying Luo, Qian Yang, Chao Liu, Yunfeng Shi, Minglong Ye, Liya Hong, Weike Su","doi":"10.1002/bmc.70049","DOIUrl":"https://doi.org/10.1002/bmc.70049","url":null,"abstract":"<div>\u0000 \u0000 <p>A novel and rapid RP-HPLC coupled with nano quantity analyte detector (NQAD) method was developed for determination of ethambutol hydrochloride and (+)2-aminobutanol in drug products. NQAD is an aerosol-based detector that can be used for the direct detection of the substance lacking ultraviolet chromophores. The two highly polar analytes were separated on a C<sub>18</sub> column with mobile phase consists of trifluoroacetic acid solution and methanol and detected with NQAD. A thorough investigation was conducted into the experimental and instrumental parameters, including the composition and ratio of mobile phase, the flow-rate of mobile phase, and nebulizer and evaporator temperature, to achieve the highest sensitivity of analytes. The method was validated as per ICH guidelines and proved to be specific, accurate, precise, linear, sensitive, and robust. The method showed adequate performance, with excellent sensitivity for limits of detection (LODs) of 5.6 and 9.8 ng for ethambutol and (+)2-aminobutanol (Impurity I), respectively. Good linearity was obtained for ethambutol and Impurity I in their concentration range both with an <i>R</i><sup>2</sup> value of 0.999. Overall, the method established in this study, utilizing a new detection technique, proved to be accurate and rapid and is applicable for directly detecting Impurity I and ethambutol in quality control.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of Anti-Biofilm Compounds From Paeoniae Radix Alba Based on Oral Biofilm Biochromatography

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-09 DOI: 10.1002/bmc.70019

Xin-Ming Liu, Yi Yu, Heng Jiang, Yu-Fei Wang, Yan Gao, Lei Xiao, Ming Liang, Jin Qi

{"title":"Screening of Anti-Biofilm Compounds From Paeoniae Radix Alba Based on Oral Biofilm Biochromatography","authors":"Xin-Ming Liu, Yi Yu, Heng Jiang, Yu-Fei Wang, Yan Gao, Lei Xiao, Ming Liang, Jin Qi","doi":"10.1002/bmc.70019","DOIUrl":"https://doi.org/10.1002/bmc.70019","url":null,"abstract":"<div>\u0000 \u0000 <p>Oral biofilms, which are known as dental plaque, are the reason for a wide range of oral and systemic diseases, which contribute to serious health risks. Paeoniae Radix Alba (PRA) is traditionally used as a folk medicine with anti-inflammatory, cardioprotective, and hepatoprotective properties. PRA is currently used in a variety of therapeutic approaches for oral diseases. Nevertheless, its inhibitory effect on oral biofilm formation and the basis for its efficacy have not been clarified. This study intended to screen the potential compounds in PRA that inhibit oral biofilm formation using biochromatography. Two biofilm models based on <i>S. mutans</i> were used to determine the inhibitory effect of PRA on biofilm formation. The extraction of PRA was divided into fractions with different polarity, the active fraction screened, and an HPLC profile constructed for the active fraction. Three potential compounds were screened using targeted oral biofilm extraction, and subsequent validation of the efficacy indicated that albiflorin is the main compound in PRA exerting anti-biofilm activity. Our results have revealed the pharmacological substance basis of PRA in inhibiting the formation of oral biofilm and provide a reference for the further use of PRA in the development of oral health products.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0