Biomedical Chromatography最新文献

{"title":"Chemical Composition Analysis of Amomi Fructus Rotundus Essential Oil at Different States by GC–MS and Its Anti-Gastritis Mechanism and Antibacterial Activity Based on Network Pharmacology and Molecular Docking","authors":"Zishuai Wen,&nbsp;Xinyi Wang,&nbsp;Yuling Ruan,&nbsp;Zhenjie Liu,&nbsp;Haiping Chen,&nbsp;Fangfang Wu,&nbsp;Huazhen Qin","doi":"10.1002/bmc.70436","DOIUrl":"10.1002/bmc.70436","url":null,"abstract":"<div>\u0000 \u0000 <p>This study analyzed the essential oils of Amomi Fructus Rotundus (from the whole fruit, pericarp, and non-moldy/moldy kernels) by GC–MS. Thirty-two compounds were identified, primarily eucalyptol, β-pinene, D-limonene, and α-pinene. The pericarp oil showed the strongest antibacterial activity, which was linked to specific compounds (e.g., 3-carene). Moldy kernels exhibited new compounds, indicating metabolic changes. Network pharmacology and molecular docking suggested anti-gastritis effects via targets (e.g., EGFR, PTGS2) and inflammation-related pathways. Additionally, in vitro assays confirmed antibacterial effects against <i>Escherichia coli</i>, <i>Staphylococcus aureus</i>, <i>Candida albicans</i>, and <i>Listeria monocytogenes</i>. This work elucidates the oil's anti-gastritis potential and antibacterial activity, supporting quality control and medicinal development.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 5","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetics, Bioavailability, and Metabolism of Zongertinib, a Novel HER2-Selective Tyrosine Kinase Inhibitor, in Rat by Liquid Chromatography Hyphenated With Electrospray Ionization Tandem Mass Spectrometry","authors":"Wenfei Sun,&nbsp;Fang Wang,&nbsp;Furui Chu,&nbsp;Zheheng Ma","doi":"10.1002/bmc.70434","DOIUrl":"10.1002/bmc.70434","url":null,"abstract":"<div>\u0000 \u0000 <p>Zongertinib, a selective human epidermal growth factor receptor 2 (HER2) tyrosine kinase inhibitor, has been approved by the US Food and Drug Administration for the treatment of non-small cell lung cancer. This study aimed to develop a sensitive and cost-effective LC–MS/MS method for quantifying zongertinib in rat plasma. Following protein precipitation with acetonitrile, samples were analyzed on an ACQUITY BEH C₁₈ column using a gradient of 0.1% formic acid and acetonitrile at 40°C within a 2-min run time. The assay demonstrated excellent linearity from 1 to 1000 ng/mL (<i>r</i> &gt; 0.995). All validation parameters—including precision, accuracy, matrix effect, recovery, and stability—met accepted criteria for bioanalytical quantification. The validated method was successfully applied to a pharmacokinetic study in rats. Additionally, metabolites in rat plasma were investigated using LC-Orbitrap-HRMS. Three metabolites were identified and structurally characterized based on accurate mass and fragmentation patterns, revealing metabolic pathways such as oxygenation, demethylation, and epoxide hydrolysis. This is the first report on the method validation for the measurement of zongertinib in biological matrices, which enables clinical development of zongertinib and can be applied for clinical pharmacokinetics and therapeutic drug monitoring in future clinical practice.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 5","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147519760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracing the Analytical Footprint of Belinostat: Exploring Pharmacology and Synthetic Framework","authors":"Sanket Chaudhari,&nbsp;Hemant Kumar Tatapudi,&nbsp;Jami Durgaganesh,&nbsp;Kishore Raju Vatsavai,&nbsp;Saisneha Kandhagatla","doi":"10.1002/bmc.70424","DOIUrl":"10.1002/bmc.70424","url":null,"abstract":"<div>\u0000 \u0000 <p>Belinostat (PXD101), a hydroxamate-class histone deacetylase inhibitor (HDACi), was approved by the US FDA for patients with relapsed or refractory peripheral T-cell lymphoma (PTCL). This review covers belinostat's pharmacological characteristics (including its mode of action), pharmacokinetics, toxicity, drug interactions, and analytical methods. Belinostat inhibits HDACs from Classes I and II, which then raises the acetylation of both histone and nonhistone proteins, resulting in growth cycle arrest and ultimately leading to the death of the malignant cells. The pharmacokinetics of belinostat include a brief elimination half-life and substantial first-pass metabolism in the liver, mostly via UGT1A1. Belinostat's efficacy has been proven in numerous clinical trials, which also revealed a certain level of cytotoxicity specific to tumor cells. Belinostat and its potential metabolites have often been qualitatively and quantitatively estimated and tracked using several analytical methods including UPLC-MS/MS, HPLC-UV, FTIR, TLC, NMR, and ESI-MS. In terms of therapeutic use of belinostat, this review demonstrates how important it is to understand the metabolism and degradation pathways of belinostat, as well as possible drug–drug interactions.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 5","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147509232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality by Design-Based Development of a Robust LC Method for Simultaneous Estimation of Process- and Degradation-Related Impurities in Rifapentine Drug Product for the Treatment of Active and Latent Tuberculosis","authors":"Siva Prasad Korikana,&nbsp;Sreenivasa Rao Battula,&nbsp;Naresh Konduru,&nbsp;Aravind Kurnool,&nbsp;Divya Kumar Vemuri,&nbsp;Venkata Lakshamana Sagar Dantinapalli","doi":"10.1002/bmc.70430","DOIUrl":"10.1002/bmc.70430","url":null,"abstract":"<p>A precise, robust, and stability-indicating liquid chromatographic (LC) method coupled with a photodiode array (PDA) detector was developed and validated for the quantitative estimation of rifapentine, an essential therapeutic agent for both active and latent tuberculosis (TB). The chromatographic separation was achieved on a YMC Pack C18 column (250 × 4.6 mm, 5 μm) using a gradient elution with Solvent A composed of phosphate buffer (pH 6.3) and acetonitrile (90:10, v/v), and Solvent B consisting of 100% acetonitrile. The optimized parameters included a flow rate of 1.0 mL min⁻¹, a column temperature of 30 °C, an injection volume of 25 μL, and UV detection at 330 nm. Method validation, conducted as per ICH Q2(R2) guidelines, confirmed excellent accuracy with recovery results between 97.0% and 103.0%. The method demonstrated linearity over a broad concentration range, from the limit of quantification (LOQ) to 200% of the target concentration, with correlation coefficients (<i>R</i>²) exceeding 0.998 for all impurities. Precision, expressed as relative standard deviation (RSD), was consistently below 5.0%. Forced degradation studies indicated that rifapentine undergoes degradation under acidic, basic, oxidative, thermal, and photolytic conditions, thus confirming the stability-indicating nature of the developed LC method.</p>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 5","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13006746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147497547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Validation of a Stability-Indicating Reversed-Phase Liquid Chromatographic Method for the Simultaneous Determination of Methylprednisolone Aceponate and Benzyl Alcohol in Cream Formulation","authors":"Aysen Kurt Cucu,&nbsp;Deniz Çıkla Yılmaz,&nbsp;Fevziye Gizem Tecimen,&nbsp;Aykut Kul","doi":"10.1002/bmc.70429","DOIUrl":"10.1002/bmc.70429","url":null,"abstract":"<div>\u0000 \u0000 <p>Methylprednisolone aceponate (MPA) is a nonhalogenated, diester corticosteroid, specifically designed to treat eczema. The amount of benzyl alcohol (BA), an excipient, is quite high in the cream formulation of MPA; therefore, the simultaneous determination of MPA, active ingredient, and BA is important. Chromatographic analysis was performed on an Agilent Poroshell 120 EC-C18 (100 × 2.1 mm 2.7 μm) column using acetonitrile–water (40:60, v/v) mixture as mobile phase with isocratic elution. The diode array detector (DAD) was set at 245 and 258 nm. The column was thermostated at 40°C ± 1°C. A flow rate of 0.5 mL/min was used to elute the analyte from the column. The calibration curve was linear in the concentration range 0.17–4.20 μg/mL for MPA and 1.50–35.0 μg/mL for BA. The <i>R</i>² values for MPA and BA were found to be 0.9989 and 0.9999, respectively. The limit of detection was found to be 0.048 μg/mL for MPA, 0.46 μg/mL for BA, and the limit of quantification was found to be 0.17 μg/mL for MPA and 1.50 μg/mL for BA. MPA was exposed to stress conditions, and the stressed samples were analyzed using the proposed method, which can effectively separate MPA from its degradation products.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 5","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147484542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Residue Behaviors and Dietary Risks of Aryloxyphenoxy Propionate Herbicides in Rice Ecosystem by UPLC-MS/MS","authors":"Nannan Pang,&nbsp;Hongyang Zhang,&nbsp;Qiyu Gong,&nbsp;Ting Zhu,&nbsp;Jiye Hu","doi":"10.1002/bmc.70431","DOIUrl":"10.1002/bmc.70431","url":null,"abstract":"<div>\u0000 \u0000 <p>Currently, aryloxyphenoxy propionate (AOPP) herbicides, famous second only to glyphosate, have been widely used worldwide, such as cyhalofop-butyl, metamifop in rice ecosystem. In this work, field trials and QuEChERS liquid chromatography tandem mass spectrometry (LC-MS/MS) gave comprehensive residues in rice ecosystem for cyhalofop-butyl, metamifop, and their metabolites. Field trials involved 12 rice varieties and 12 sites with different climates and conditions. The determination method was reliable with average recoveries of seven analytes in brown rice, husk, and straw between 90.2% and 110.0%, with <i>RSDs</i> ≤ 16.0%. LOQs for seven analytes were lower than 0.01 mg/kg. Their degradation rates were &lt; 21.7% at all storage intervals (168 days). Residues levels of seven analytes were consistently below their respective MRLs (&lt; 0.01 mg/kg, China). Residue levels of cyhalofop-butyl (including cyhalofop and cyhalofop diacid) and metamifop (HPFMPA, HFMPA, and 6-CBO) were consistently below 0.03 and 0.04 mg/kg, respectively. Related chronic and acute risk quotients for cyhalofop-butyl, metamifop were &lt; 1, indicating acceptable dietary risks. And children are exposed in environments with relatively higher risks. Due to the lack of reported residues, field trials, and dietary risk assessment, our work is useful for AOPP herbicides monitoring and related MRL issuing and provide data for herbicide management, food safety, and human health.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 5","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147490559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical Quality by Design-Guided Optimisation and Validation of a Short-Run Stability-Indicating Reverse-Phase Ultra-Fast Liquid Chromatography (RP-UFLC) Method for the Quantitation of Nonivamide","authors":"Phindile Mahlangu,&nbsp;Madan S. Poka,&nbsp;Pedzisai A. Makoni,&nbsp;Bwalya A. Witika","doi":"10.1002/bmc.70423","DOIUrl":"10.1002/bmc.70423","url":null,"abstract":"<p>Nonivamide (NON), a capsaicin analogue with similar pharmacological effects, is used in pain management, yet limited information exists on its accurate quantification by reverse-phase liquid chromatography. A cost-effective, efficient and precise RP-HPLC method was developed, optimised using response surface methodology (central composite design), validated and assessed for greenness. Naproxen served as the internal standard. Acetonitrile (ACN) with a potassium phosphate buffer achieved separation, with detection at 285 nm. Critical analytical attributes included retention times, resolution and solvent composition. Optimised conditions were 41.0% v/v of ACN, a flow rate of 1.5 mL/min and a buffer of pH 4. Under these conditions, NON eluted at 6.8 min with a resolution of 13 and %RSD &lt; 5%, aligning with model predictions, confirming robustness and system suitability. Greenness evaluation (AGREE) yielded a score of 0.72 (good–very good). Method validation followed the ICH guidelines: linearity of 2–200 μg/mL, LOD of 0.67 μg/mL and LOQ of 2 μg/mL. Precision and accuracy were within acceptable limits (&lt; 5%). Stress studies revealed NON stability under most conditions, except alkaline and oxidative stress, whereas specificity was confirmed by detection in the presence of degradants. Overall, the developed method is robust, reproducible, reliable and environmentally considerate, enabling accurate NON quantification from pharmaceutical bulk.</p>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 5","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12998193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147472513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical Profiling and Biological Activity of Copaiba Oil-Resin Samples and Development of HPLC-DAD Method for the Analysis of β-Caryophyllene","authors":"Gabriel de A. P. Graça,&nbsp;Cláudia G. Silva,&nbsp;Denise de O. Scoaris,&nbsp;Juliana Rodrigues de Vasconcelos,&nbsp;André Santos Alkimim,&nbsp;Vanessa C. F. Mosqueira,&nbsp;Luciana S. Salomon,&nbsp;Pietra Piotto Marcellini,&nbsp;Isabelle Renata Martins da Silva,&nbsp;Janete S. C. Santos,&nbsp;Andreia Fonseca Silva,&nbsp;Carolina P. S. Moreira,&nbsp;Júlio C. D. Lopes,&nbsp;Vera L. de Almeida","doi":"10.1002/bmc.70416","DOIUrl":"10.1002/bmc.70416","url":null,"abstract":"&lt;p&gt;The &lt;i&gt;Copaifera&lt;/i&gt; genus belongs to the Fabaceae family, and the species occur mainly in Central and South America (Trindade et al. &lt;span&gt;2018&lt;/span&gt;; Frazão et al. &lt;span&gt;2024&lt;/span&gt;). In Brazil, the species of copaiba trees can be found in all the regions (Flora e Funga do Brasil &lt;span&gt;2025&lt;/span&gt;). Although many research groups have studied the taxonomy of the &lt;i&gt;Copaifera&lt;/i&gt; genus, its botanical identification is difficult. According to Trindade et al. (&lt;span&gt;2018&lt;/span&gt;), it has been attributed to their intricate floral morphology and absence of reproductive structures in the samples.&lt;/p&gt;&lt;p&gt;The &lt;i&gt;Copaifera&lt;/i&gt; species are also known as ‘Copaiba balsam’, ‘Copaiba’, ‘Angelim copaiba’, ‘Copaiba branca’, ‘Iguapo copaiba’, ‘Jutaí copaiba’, ‘Copaiba vermelha’, ‘True copaiba’, ‘Capaibarana’, ‘Copauba’, ‘Mari-Mari’ and ‘Óleo vermelho’ (Tappin et al. &lt;span&gt;2004&lt;/span&gt;; Arruda et al. &lt;span&gt;2019&lt;/span&gt;; Frazão et al. &lt;span&gt;2024&lt;/span&gt;). The different species are used traditionally for their anti-inflammatory, antiseptic and healing properties. In addition, its oil-resin has been used to treat a variety of other problems, such as urinary, skin, rheumatic and respiratory diseases (Tappin et al. &lt;span&gt;2004&lt;/span&gt;; Pieri et al. &lt;span&gt;2009&lt;/span&gt;; Arruda et al. &lt;span&gt;2019&lt;/span&gt;; Lee et al. &lt;span&gt;2023&lt;/span&gt;; Leandro et al. &lt;span&gt;2024&lt;/span&gt;). Several other uses of copaiba oil-resin have been described, including as a raw material for biodiesel production, as a fuel, in the wood, photographic and paper industries and for the manufacture of paints (Trindade et al. &lt;span&gt;2018&lt;/span&gt;; Frazão et al. &lt;span&gt;2024&lt;/span&gt;). According to Silva-Junior and co-workers (2023), the copaiba oil-resin is one of the main non-timber forest products (NTFP) commercialized in the popular market in the Amazon State.&lt;/p&gt;&lt;p&gt;However, only one herbal medicine is registered with the Brazilian Health Regulatory Agency (ANVISA, consulted on 31/07/&lt;span&gt;2025&lt;/span&gt;) for the commercial use of copaiba oil as an anti-inflammatory. In contrast, a variety of products, including crude oil, dietary supplements (capsules) and a wide range of cosmetics containing copaiba oil-resin in combination with other plants, are currently available for online sales and at public markets.&lt;/p&gt;&lt;p&gt;Supplies of this exuded oil-resin come from natural populations of copaiba, which are slow-growing plants and, therefore, limited in availability. The oil-resin is obtained through artisanal procedures and may exhibit variability in its phytochemical profile because of biotic and abiotic factors. The phytochemical profile of copaiba oil-resin can vary depending on the species, season and harvesting location. Moreover, its chemical composition may be affected by epigenetic and genetic responses to abiotic stress. Sesquiterpenes and diterpenes are the main classes of compounds detected in oil-resin (Veiga Junior. et al. &lt;span&gt;1997&lt;/span&gt;; Veiga Junior and Pinto &lt;span&gt;2002&lt;/span&gt;; Lee et al. &lt;span&gt;2023&lt;/span&gt;; Fraz","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 5","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12993705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147466263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolving Analytical Methods for the Quantification of Methocarbamol: A Critical Review","authors":"Hemn A. H. Barzani,&nbsp;Rebaz Anwar Omer,&nbsp;Nergz Bayiz Abdulrahman,&nbsp;Amjad Mahmood Qadir,&nbsp;Seerwan Hamadameen Sulaiman","doi":"10.1002/bmc.70419","DOIUrl":"10.1002/bmc.70419","url":null,"abstract":"<div>\u0000 \u0000 <p>Methocarbamol (MT), a centrally acting muscle relaxant, presents significant analytical challenges due to its low ultraviolet (UV) absorptivity, rapid metabolism, and pronounced pH-dependent instability. The presence of guaifenesin-related impurities and interference from co-formulated analgesics further complicate its detection in pharmaceutical, biological, and environmental matrices. This review compiles peer-reviewed English-language studies indexed in Scopus, Web of Science, PubMed, ScienceDirect, and Google Scholar and provides a critical assessment of MT quantification methods developed over the past 25 years. Evidence shows that optimized high-performance liquid chromatography (HPLC), particularly fluorescence-based systems, provides the highest sensitivity and selectivity, achieving sub-ng/mL detection and overcoming limitations of UV assays, such as signal suppression and narrow linear ranges. Electrochemical approaches using CNT-, graphene-, MOF-, and magnetic-nanocomposite-modified electrodes enhance redox discrimination and enable nM–μM detection. Chemometric-assisted spectroscopic techniques offer rapid, low-cost analysis suitable for simple formulations but lack reliability in complex matrices. Across all platforms, strict pH control, rapid processing, and selective extraction methods—such as solid-phase extraction (SPE), molecularly imprinted polymer (MIP), and dispersive liquid–liquid microextraction (DLLME) remain essential to reduce degradation and matrix interference. Future progress will rely on miniaturized microfluidic systems, improved stability-indicating chromatography, and intelligent electrochemical sensors capable of multiplex quantification in challenging samples.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 5","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147466285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrophilic Interaction Liquid Chromatography (HILIC) as a Powerful Tool for the Determination of Antiepileptic Drugs: A Critical Review","authors":"Haia Alkeifi","doi":"10.1002/bmc.70427","DOIUrl":"10.1002/bmc.70427","url":null,"abstract":"<div>\u0000 \u0000 <p>Epilepsy is a clinically significant neurological disorder requiring lifelong pharmacotherapy with antiepileptic drugs (AEDs). Precise quantification of these agents in pharmaceutical formulations and biological matrices is essential to ensure therapeutic efficacy and patient safety. Although high-performance liquid chromatography (HPLC) is the gold standard for AED determination, hydrophilic interaction liquid chromatography (HILIC) has emerged as a powerful alternative, especially for highly polar and hydrophilic AEDs. HILIC provides several advantages, including superior peak resolution, enhanced reproducibility, and reduced analysis time; yet, its application in AED analysis remains relatively underexplored. This review critically evaluates the literature regarding HILIC's application in this field, focusing on chromatographic parameters, detection strategies, and method validation. Emphasis is placed on analytical performance, specifically sensitivity, accuracy, precision, and speed. Collectively, the evidence underscores HILIC's potential as a robust and efficient platform for the routine monitoring and quality control of AEDs, highlighting an underutilized approach to enhancing AED determination. However, technical constraints, including prolonged column equilibration and the complexity of retention mechanisms, remain challenges for its widespread routine implementation.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 5","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147466290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}