Biomedical Chromatography最新文献_第2页

Investigation Into the Chemical Constituents and Cytotoxic Effects on Lung Cancer Cells of the Rhizomes of Polygonum bistorta L. and Polygonum paleaceum Wall. 历史蓼根茎和古蓼根茎化学成分及对肺癌细胞毒作用的研究。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-09-22 DOI: 10.1002/bmc.70225

Lingxia Xu, Ting Tan, Yun Luo

{"title":"Investigation Into the Chemical Constituents and Cytotoxic Effects on Lung Cancer Cells of the Rhizomes of Polygonum bistorta L. and Polygonum paleaceum Wall.","authors":"Lingxia Xu, Ting Tan, Yun Luo","doi":"10.1002/bmc.70225","DOIUrl":"10.1002/bmc.70225","url":null,"abstract":"<div>\u0000 \u0000 <p>The medicinal herb market frequently blends the rhizome of <i>Polygonum bistorta</i> L. (RPB) with the rhizome of <i>Polygonum paleaceum</i> Wall. (RPP) for sale. Therefore, it is crucial to investigate the chemical compositions and the cytotoxic effects on lung cancer of RPB and RPP. In this study, we utilized ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS), employing diagnostic fragment ions and neutral loss techniques, to identify the chemical compositions in RPB and RPP. Furthermore, the cytotoxicity of RPB and RPP on H1299 and A549 cells was evaluated using the cell counting kit-8 (CCK-8) assay. A total of 90 compounds were tentatively characterized in RPB and RPP, including 25 gallic acid derivatives, 46 catechin derivatives, and 19 other compounds, with seven compounds verified using authentic standards. The chemical compositions of RPB and RPP were found to be largely similar, with significant differences observed only in the content of individual components. RPB and RPP exhibit significant cytotoxicity against H1299 cells, with IC<sub>50</sub> values of 163.8 and 187.7 μg/mL, respectively. However, their cytotoxicity was relatively lower against A549 cells. The results indicate that there is no significant difference in the chemical composition or lung cancer cytotoxicity between RPB and RPP.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 11","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Posaconazole: A Triazole Antifungal Drug: Estimation of Chiral Isomers Using a Chiral Chromatography 泊沙康唑：一种三唑类抗真菌药物：用手性色谱法测定手性异构体。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-09-19 DOI: 10.1002/bmc.70220

Hari Hara Pratap Kumar Grandhi, Ramesh Kola, Ramarao Abburi

{"title":"Posaconazole: A Triazole Antifungal Drug: Estimation of Chiral Isomers Using a Chiral Chromatography","authors":"Hari Hara Pratap Kumar Grandhi, Ramesh Kola, Ramarao Abburi","doi":"10.1002/bmc.70220","DOIUrl":"10.1002/bmc.70220","url":null,"abstract":"<div>\u0000 \u0000 <p>Posaconazole is a second-generation triazole antifungal agent widely used in the prevention and treatment of invasive fungal infections, particularly in immune-compromised patients. The complex molecular architecture of Posaconazole, characterized by a triazole ring, difluorophenyl moiety, tetrahydrofuran ring, and a piperazine group, is designed to optimize antifungal activity, pharmacokinetics, and bioavailability. Crucially, it contains four chiral centers, giving rise to up to 16 (2<sup>4</sup>) stereoisomers, among which only one is therapeutically active. This study emphasizes the necessity of chiral purity analysis due to the profound impact of stereochemistry on drug safety, efficacy, and metabolism. The current study deals with the development and validation of a simple, robust chiral HPLC method using a Chiralpak IB-3 column with a chiral selector of Cellulose tris (3,5-dimethylphenylcarbamate) and dimensions of 250 mm × 4.6 mm, 3 μm of particle size. A 5:25:61:9:0.1 <i>v</i>/<i>v</i>/<i>v</i>/<i>v</i>/<i>v</i> ratio of ethanol, isopropyl alcohol, n-hexane, dichloromethane, and diethanolamine is used as a mobile phase for the quantification of chiral isomers. The method demonstrates strong performance in terms of system suitability, specificity, sensitivity, linearity, accuracy, precision, robustness, and solution stability. This work provides a critical analytical tool for quality control and regulatory compliance and underscores the broader importance of stereoisomeric evaluation in pharmaceutical development.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 11","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145085170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Proteomics in the Study of Toxicology and Toxic Markers of Traditional Chinese Medicines 蛋白质组学在中药毒理学及毒性标志物研究中的应用

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-09-11 DOI: 10.1002/bmc.70216

Yang Yadi, Shang Ziyi, Hu Bin, Sun Ye, Li Jingjing, Ma Yujin, Jiang Hongwei, Liu Chuanxin, Li Xuejiao

{"title":"Application of Proteomics in the Study of Toxicology and Toxic Markers of Traditional Chinese Medicines","authors":"Yang Yadi, Shang Ziyi, Hu Bin, Sun Ye, Li Jingjing, Ma Yujin, Jiang Hongwei, Liu Chuanxin, Li Xuejiao","doi":"10.1002/bmc.70216","DOIUrl":"10.1002/bmc.70216","url":null,"abstract":"<div>\u0000 \u0000 <p>Proteomics is an omics emerging after genomics and transcriptomics, which plays a vital role in the fields of drug metabolism, pharmacotoxicology, toxicology, toxicodynamics, safety evaluation, and clinical medication. It provides a powerful tool for toxicity evaluation of TCM and its components, by systematically revealing toxicity mechanisms, identifying toxic biomarkers, and supporting toxicity prediction. This technology, by providing dynamic and functional information at the protein level, reveals key enzyme activities in drug metabolism and individual differences, identifies early toxicological markers and mechanisms, enhances safety evaluation comprehensiveness, and promotes clinical medication precision. This paper reviewed the application of proteomics in the research of toxicological mechanisms, toxicity biomarkers, and toxicity prediction of TCM to provide a reference for the study of toxicology and toxicity markers of TCM. In the future, proteomics will develop toward higher resolution, combine with artificial intelligence (AI) for deep data mining, and enhance multiomics data integration, enabling breakthroughs in dynamic disease monitoring, precise toxicity prediction, target discovery, and individualized diagnosis.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145037836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality Difference Analysis of Pomegranate Peels Based on Multidimensional Fingerprint With Chemical Pattern Recognition 基于多维指纹化学模式识别的石榴果皮品质差异分析。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-09-11 DOI: 10.1002/bmc.70218

Dandan Zheng, Hui Lv, Xiao Lin, Chengshi Ding, Xiaohui Feng, Yonghui Ji, Xiandong Lv, Jing Ma, Jinglong Wang

{"title":"Quality Difference Analysis of Pomegranate Peels Based on Multidimensional Fingerprint With Chemical Pattern Recognition","authors":"Dandan Zheng, Hui Lv, Xiao Lin, Chengshi Ding, Xiaohui Feng, Yonghui Ji, Xiandong Lv, Jing Ma, Jinglong Wang","doi":"10.1002/bmc.70218","DOIUrl":"10.1002/bmc.70218","url":null,"abstract":"<div>\u0000 \u0000 <p>To evaluate the quality of pomegranate peels from different cultivars, pomegranate peel samples from 47 cultivars were compared and classified based on fingerprints and chemical components obtained using HPLC-PDA-MS/MS combined with chemometric methods. Three pattern recognition methods, namely, hierarchical cluster analysis, principal component analysis, and partial least square–discriminant analysis, were used to establish classification models. Results showed that the contents of 10 components from pomegranate peel were determined. A total of 21 liquid chromatographic characteristic peaks and 26 mass spectra characteristic peaks were obtained from HPLC-PDA-MS/MS fingerprint analysis. Pomegranate peel samples from 47 cultivars were divided into three groups, and four and three chemical markers were labeled with identification based on HPLC and MS/MS models, respectively. In conclusion, this study firstly establishes an adequate method for quantitative profiling across multiple active ingredients for quality assessment and prediction of pomegranate peels of a specific cultivar set from different production areas and provides novel ideas for the research of Traditional Chinese Medicine.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of Trans- and Cis-Vitamin K1 Isomers in Human Plasma by a Rapid and Sensitive LC–MS/MS Method With Surrogate Matrix 快速、灵敏的LC-MS/MS替代基质法定量人血浆中反式和顺式维生素K1异构体

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-09-10 DOI: 10.1002/bmc.70209

Yi Jin, Zibin Song, Hanqi Du, Jing Zhuo, Xingyue Zhang, Yue Wang, Haiyan Xu, Xiumei Lu

{"title":"Quantification of Trans- and Cis-Vitamin K1 Isomers in Human Plasma by a Rapid and Sensitive LC–MS/MS Method With Surrogate Matrix","authors":"Yi Jin, Zibin Song, Hanqi Du, Jing Zhuo, Xingyue Zhang, Yue Wang, Haiyan Xu, Xiumei Lu","doi":"10.1002/bmc.70209","DOIUrl":"10.1002/bmc.70209","url":null,"abstract":"<div>\u0000 \u0000 <p>A rapid and specific liquid chromatography–tandem mass spectrometry method with a wide linear range was developed and validated for the simultaneous quantification of Vitamin K1 (VK1) trans- and cis- isomers in human plasma. Bovine serum albumin solution (15%) served as a surrogate matrix for preparing the calibrators to establish the quantitative curves. After liquid–liquid extraction, VK1 trans- and cis- isomers in plasma samples were separated on a ChromCore C30 column (15 cm × 4.6 mm, 3 μm) using water: methanol: acetonitrile: formic acid (5:80:20:0.1, v/v/v/v) as the mobile phase. Mass spectrometry detection was performed in positive ion mode with atmospheric pressure chemical ionization (APCI) interface by multiple reaction monitoring (MRM) method. The calibration curves were linear over the range of 0.400–6000 ng/mL for the trans-isomer and 0.400–1200 ng/mL for the cis-isomer (<i>r</i> ≥ 0.994). The intra- and inter-day precision was below 9.55% in terms of relative standard deviation (RSD%) and the accuracy was within ±11.24% in terms of relative error (RE%). The selectivity, sensitivity, extraction recovery, matrix effect, dilution reliability, and stability met the acceptable criteria. The reliable LC–MS/MS method for concurrent detection of VK1 trans- and cis- isomers was successfully employed in a pharmacokinetic study in healthy Chinese volunteers after oral administration of 10 mg VK1.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Stability and Comprehensive Metabolite Profiling of Chrysotobibenzyl in Human Liver Microsomes and Hepatocytes Employing UPLC-MS/MS and UPLC-Orbitrap-HRMS Analysis 基于UPLC-MS/MS和UPLC-Orbitrap-HRMS分析的人肝微粒体和肝细胞中黄氧联苯的代谢稳定性和综合代谢物分析

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-09-10 DOI: 10.1002/bmc.70217

Xuanwei Liu, Junsheng Ge, Nana Zhao, Yeji Liang, Yu Liu, Xiaoman Wang, Lihua Tan

{"title":"Metabolic Stability and Comprehensive Metabolite Profiling of Chrysotobibenzyl in Human Liver Microsomes and Hepatocytes Employing UPLC-MS/MS and UPLC-Orbitrap-HRMS Analysis","authors":"Xuanwei Liu, Junsheng Ge, Nana Zhao, Yeji Liang, Yu Liu, Xiaoman Wang, Lihua Tan","doi":"10.1002/bmc.70217","DOIUrl":"10.1002/bmc.70217","url":null,"abstract":"<div>\u0000 \u0000 <p>Chrysotobibenzyl, a bioactive ingredient from <i>Dendrobium chrysotoxum</i>, exhibits potent anti-tumor activity. However, its metabolic profiles remain unelucidated. This study aimed to disclose the metabolic fates of chrysotobibenzyl using human liver fractions. In vitro metabolism of chrysotobibenzyl was assessed using human liver microsomes and hepatocytes. The concentration of unchanged parent compound was quantified using a validated UPLC-MS/MS method. Metabolite profiling was achieved by Q-Exactive Orbitrap HRMS combined with Compound Discoverer software using a mass defect filtering approach, with structures characterized by accurate mass measurement and fragmentation pattern interpretation. Chrysotobibenzyl exhibited poor metabolic stability in human liver microsomes (<i>t</i><sub>1/2</sub>: 16.24 min) and hepatocytes (<i>t</i><sub>1/2</sub>: 44.35 min). Totally, 36 metabolites were identified, comprising 19 phase I metabolites, 10 glucuronide conjugates, and seven GSH adducts. Using reference standards, M22, M25, M27, and M28 were unambiguously identified as moscatilin, chrysotoxine, erianin, and crepidatin, respectively. The detection of GSH conjugates indicated the formation of reactive metabolites, including <i>ortho</i>-quinone and quinone-methide intermediates. This study demonstrates the effectiveness of UPLC-MS/MS and UPLC-Orbitrap-HRMS platforms for metabolic profiling. Metabolic pathways include demethylation, hydroxylation, dehydrogenation, glucuronidation, and GSH conjugation. These findings provide critical insights into the metabolism of chrysotobibenzyl in humans, enhancing our understanding of its biological activity.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Pharmacokinetics and Oral Bioavailability of Saikosaponin A in Rats Using a Validated UFLC–MS/MS Method 鸢尾皂苷A在大鼠体内的药动学及口服生物利用度的验证UFLC-MS/MS方法

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-09-10 DOI: 10.1002/bmc.70219

Zhongwen Sun, Chao Qiu, Yiqiang An, Zhengyuan Yan, Caifu Li

{"title":"Characterization of Pharmacokinetics and Oral Bioavailability of Saikosaponin A in Rats Using a Validated UFLC–MS/MS Method","authors":"Zhongwen Sun, Chao Qiu, Yiqiang An, Zhengyuan Yan, Caifu Li","doi":"10.1002/bmc.70219","DOIUrl":"10.1002/bmc.70219","url":null,"abstract":"<div>\u0000 \u0000 <p>Saikosaponin A (SSa) is an oleanane type triterpenoid saponin isolated from Radix Bupleuri (<i>Bupleurum chinense</i> DC). While SSa has demonstrated significant pharmacological activities including anti-inflammatory, antioxidant, and antidepressant effects, its pharmacokinetic profile remains poorly characterized. This study developed and validated a sensitive LC–MS/MS method for quantifying SSa in rat plasma. After acetonitrile-mediated protein precipitation, SSa and the internal standard (IS) were separated on a Waters Acquity BEH C18 column using MS detection operated in negative multiple reaction monitoring (MRM) mode. The assay was linear over the concentration range of 2–1000 ng/mL with satisfactory validation parameters of intra-and inter-day precision (3.50%–10.01%) and accuracy (−5.93% to −2.68%), extraction recovery (73.75%–82.50%), stability, and matrix effect (88.49%–103.64%). Application in pharmacokinetic studies revealed distinct administration-related characteristics. Intravenous administration (5 mg/kg) resulted in high clearance with an elimination half-life (<i>t</i><sub>1/2</sub>) of 2.29 h and was accompanied by hemolysis. Oral administration at doses of 50, 100, and 200 mg/kg showed dose-dependent systemic exposure with consistently low bioavailability (0.04%). The limited absorption is likely attributable to poor gastrointestinal permeability and extensive metabolism mediated by intestinal microbiota and hepatic first-pass effects, as indicated by the extremely low system exposure. These findings provide useful information for optimizing SSa therapeutic applications.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145032540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum Metabolomics of Patients with Hepatic Cystic Echinococcosis 肝囊性包虫病患者血清代谢组学研究

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-09-08 DOI: 10.1002/bmc.70180

Yisimayili Aimaiti, Kahaer Tuerxun, Yuan-Quan Wu, Abudoukeyimu Yasheng, Irshat Ibrahim, Qi-Lin Xu, Muzaipaer Muhetajiang, Tuerganaili Aji

{"title":"Serum Metabolomics of Patients with Hepatic Cystic Echinococcosis","authors":"Yisimayili Aimaiti, Kahaer Tuerxun, Yuan-Quan Wu, Abudoukeyimu Yasheng, Irshat Ibrahim, Qi-Lin Xu, Muzaipaer Muhetajiang, Tuerganaili Aji","doi":"10.1002/bmc.70180","DOIUrl":"10.1002/bmc.70180","url":null,"abstract":"<p>Hepatic cystic echinococcosis (HCE), a liver manifestation of hydatid disease, is among the 17 neglected tropical diseases (NTDs) prioritized by the WHO for eradication by 2025. Although imaging and serological tests are currently the main diagnostic approaches for HCE, they have notable limitations in sensitivity and specificity. Here, we applied liquid chromatography–tandem mass spectrometry (LC-MS/MS)–based metabolomic profiling to uncover differential metabolites and highlight disrupted metabolic pathways, aiming to identify candidate biomarkers for HCE diagnosis. Ten patients diagnosed with HCE were enrolled in the case group. Thirteen healthy individuals were included as controls. Serum metabolomic profiling was performed using LC-MS/MS. Differences in metabolite profiles between the two groups were analyzed employing both univariate and multivariate statistical methods. A total of 20 differential metabolites were significantly altered in the HCE group compared to the controls (<i>p</i> < 0.05, VIP > 1.0). Pathway and enrichment analyses revealed that these metabolites were mainly involved in 8 metabolic pathways, suggesting their potential as candidate biomarkers for HCE diagnosis. These findings enhance our understanding of the metabolic alterations associated with HCE and provide a foundation for further investigation into the disease's pathogenesis and the development of metabolite-based diagnostic tools.</p>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/bmc.70180","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145012621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomics Study on the Antitumor Effect of Osthole on HepG2 Cells Using UPLC-Q-TOF-MS/MS UPLC-Q-TOF-MS/MS技术研究蛇床子素对HepG2细胞的抗肿瘤作用

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-09-03 DOI: 10.1002/bmc.70215

Wenhan Lin, Lu Chen, Zijing Zhang, Xinxin Miao, Lulu Zhao, Yameng Zhu, Huizi Ouyang, Jun He

{"title":"Metabolomics Study on the Antitumor Effect of Osthole on HepG2 Cells Using UPLC-Q-TOF-MS/MS","authors":"Wenhan Lin, Lu Chen, Zijing Zhang, Xinxin Miao, Lulu Zhao, Yameng Zhu, Huizi Ouyang, Jun He","doi":"10.1002/bmc.70215","DOIUrl":"10.1002/bmc.70215","url":null,"abstract":"<div>\u0000 \u0000 <p>Hepatocellular carcinoma (HCC) stands as a globally common malignancy with persistently elevated incidence and mortality rates. It is known for being able to resist conventional chemotherapy. There is currently growing interest in natural products for the treatment of HCC, owing to their significant antitumor activity. Osthole, a naturally occurring O-methylated coumarin in <i>Cnidium monnieri</i> (L.) Cusson, showed potent antitumor effects on various types of cancer. It plays a therapeutic role in HCC by inducing apoptosis and inhibiting proliferation. Nevertheless, the anti-HCC effect of osthole at the molecular level needs to be investigated. In this study, an ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS)–based cell metabolomics approach was established to investigate the antitumor effect of osthole on HepG2 cells. The effect of osthole on the viability of HepG2 cells was found to be concentration and time-dependent. Moreover, 43 differential metabolites were identified, involving the metabolic pathways of sphingolipid, glycerophospholipid, and tryptophan. It is suggested that osthole may have an anti-HCC effect by affecting the function of normal cells, altering important cellular properties, reducing cellular activity, and inducing cellular apoptosis.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144935024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Method for Analysis of Free and Total Ropivacaine in Dog Plasma Using UHPLC–MS/MS 犬血浆中游离罗哌卡因和总罗哌卡因的UHPLC-MS /MS分析方法

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-09-03 DOI: 10.1002/bmc.70214

Natali Verdier, Karin Hummel, Ebrahim Razzazi-Fazeli

{"title":"A Method for Analysis of Free and Total Ropivacaine in Dog Plasma Using UHPLC–MS/MS","authors":"Natali Verdier, Karin Hummel, Ebrahim Razzazi-Fazeli","doi":"10.1002/bmc.70214","DOIUrl":"10.1002/bmc.70214","url":null,"abstract":"<p>Ropivacaine is a local anesthetic commonly used in veterinary anesthesia. A liquid chromatography–mass spectrometry (LC–MS) method was developed to quantify free and total ropivacaine in dog plasma, which included rapid equilibrium dialysis. The method was validated for selectivity, specificity, matrix effect, calibration curve and range, accuracy and precision, carry-over, stability, and reinjection reproducibility according to the International Conference on Harmonization M10 guidelines. After ultra-high performance liquid chromatographic (UHPLC) separation, detection and quantification of ropivacaine was performed using a triple quadrupole tandem mass spectrometer with electrospray ionization. LC–MS method validation was carried out in a range of 0.05–1000 ng/mL ropivacaine in dog plasma in two dilutions (1:1 and 1:4). The precision and accuracy of the method were determined at four concentration levels and ranged from 0.40% to 5.30% and 85.50% to 113.30%, respectively. The lower limit of quantification was as low as 0.30 and 0.05 ng/mL, for the quantitation of protein-bound (1:4) and free (1:1) ropivacaine, respectively. All validation parameters met acceptance criteria. This UHPLC–MS/MS method was successfully applied in a clinical study that involved the intraperitoneal instillation of ropivacaine to anesthetized dogs and can be used to quantify free and total ropivacaine in dog plasma.</p>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/bmc.70214","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144935023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0