Biomedical Chromatography最新文献_第2页

Pharmacokinetic Study of Eight Bioactive Components of Buyang Huanwu Decoction in LPS Induced Chronic Inflammatory and Control Rats Using UPLC-MS/MS 补阳还五汤8种活性成分在LPS诱导的慢性炎症及对照大鼠体内的药动学研究

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-21 DOI: 10.1002/bmc.70117

Li Jiang, Yanling Xiong, Weiwei Wang, Yuting Zheng, Liping Huang, Yuhui Liu, Guoliang Xu

{"title":"Pharmacokinetic Study of Eight Bioactive Components of Buyang Huanwu Decoction in LPS Induced Chronic Inflammatory and Control Rats Using UPLC-MS/MS","authors":"Li Jiang, Yanling Xiong, Weiwei Wang, Yuting Zheng, Liping Huang, Yuhui Liu, Guoliang Xu","doi":"10.1002/bmc.70117","DOIUrl":"https://doi.org/10.1002/bmc.70117","url":null,"abstract":"<div>\u0000 \u0000 <p>Inflammation plays a pivotal role in the pathogenesis of numerous chronic inflammatory diseases, including cardiovascular diseases. Buyang Huanwu decoction (BYHWD) is widely employed to treat chronic inflammation and related disorders. However, the pharmacokinetics of BYHWD's active compounds under inflammatory conditions remain unclear. This study aims to investigate the comparative pharmacokinetics of eight bioactive components in control and LPS-induced chronic inflammatory rats following oral administration of BYHWD. A chronic inflammatory rat model was established by administering low-dose lipopolysaccharide (LPS, 200 μg<b>/</b>kg) via tail vein injection. An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) method was developed for simultaneous quantification of eight major bioactive components of BYHWD in rat plasma. Subsequently, the pharmacokinetic profiles of these analytes were characterized in both control and inflammatory rats. Excellent linearity (<i>R</i><sup>2</sup> > 0.9958) was observed for all analytes in rat plasma samples. Absolute recoveries ranged from 50.84% to 111.86%, and matrix effects varied between 85.04% and 110.14%. The method met the criteria for intra- and inter-day precision, accuracy, and stability. In conclusion, the established UPLC-MS/MS method reliably investigated the pharmacokinetic characteristics of BYHWD's bioactive components and indicate that chronic inflammation may reduce the absorption of these compounds, providing valuable insights into BYHWD's clinical applications.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Natural Deep Eutectic Solvents' Extraction Process From Atractylodes macrocephala–Astragali Radix Using the Dual Mathematical Model and Its Activity Evaluation 双数学模型优化天然深共晶溶剂提取白术黄芪的工艺及活性评价

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-21 DOI: 10.1002/bmc.70122

Wenlong Huang, Qingling Meng, Mingjiang Mao, Boxuan Wang, Chenhuan Shentu, Xiaohong Li, Jie Hao, Yujin Zhao, Jiaying Zhu, Shijie Dai, Xiaofeng Yuan

{"title":"Optimization of Natural Deep Eutectic Solvents' Extraction Process From Atractylodes macrocephala–Astragali Radix Using the Dual Mathematical Model and Its Activity Evaluation","authors":"Wenlong Huang, Qingling Meng, Mingjiang Mao, Boxuan Wang, Chenhuan Shentu, Xiaohong Li, Jie Hao, Yujin Zhao, Jiaying Zhu, Shijie Dai, Xiaofeng Yuan","doi":"10.1002/bmc.70122","DOIUrl":"https://doi.org/10.1002/bmc.70122","url":null,"abstract":"<div>\u0000 \u0000 <p>Natural deep eutectic solvents (NADESs) have garnered significant attention for their application in the extraction of natural products. This study proposes a strategy to enhance the extraction efficiency of target compounds from the <i>Atractylodes macrocephala</i> Koidz–<i>Astragali Radix</i> (AM-AR) combination, and the crude extracts were employed for subsequent in vitro and in vivo investigations. The extraction efficiencies of nine NADESs and conventional solvents were compared for isolating calycosin 7-O-glucoside, ononin, quercetin, and atractylenolide III using high-performance liquid chromatography (HPLC) analysis. The extraction parameters were then optimized through response surface methodology (RSM) and genetic algorithm–back propagation neural network (GA-BPNN) models. In addition, the antioxidant activities were evaluated in vitro using assays such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) free radical scavenging, and ferric reducing antioxidant power (FRAP). An in vivo rat model of middle cerebral artery occlusion (MCAO) was utilized to assess the biological activities. Among the tested solvents, NADES-7 exhibited the highest extraction efficiency, achieving a comprehensive evaluation value of 0.173 and was selected for subsequent experiments. These findings indicate that NADESs can serve as promising alternative solvents for extracting natural products from traditional Chinese medicine (TCM).</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144100940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platycodon grandiflorum Attenuates Non-Alcoholic Fatty Liver Disease in Rats Through Regulating Inflammatory Response via PPARγ and TLR4/NF-κB Signaling Pathway 桔梗通过PPARγ和TLR4/NF-κB信号通路调节炎症反应减轻大鼠非酒精性脂肪性肝病

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-20 DOI: 10.1002/bmc.70111

Ming Dang, Jie Zhang, Run-Xin Ren, Mao-Mao Fu, Kang-Jie Li, Huan-Qing Gao, Qiao Zhang, Jing Wang, Jing Sun, Xiao-Fei Zhang, Chang-Li Wang, Meng-Meng Zhang, Chong-Bo Zhao

{"title":"Platycodon grandiflorum Attenuates Non-Alcoholic Fatty Liver Disease in Rats Through Regulating Inflammatory Response via PPARγ and TLR4/NF-κB Signaling Pathway","authors":"Ming Dang, Jie Zhang, Run-Xin Ren, Mao-Mao Fu, Kang-Jie Li, Huan-Qing Gao, Qiao Zhang, Jing Wang, Jing Sun, Xiao-Fei Zhang, Chang-Li Wang, Meng-Meng Zhang, Chong-Bo Zhao","doi":"10.1002/bmc.70111","DOIUrl":"https://doi.org/10.1002/bmc.70111","url":null,"abstract":"<div>\u0000 \u0000 <p>Non-alcoholic fatty liver disease (NAFLD) has been considered a main health concern worldwide, and <i>Platycodon grandiflorum</i> (PG) has been traditionally employed in ethnopharmacology for managing hepatic metabolic disorders. This study aimed to elucidate the potential effect of PG on NAFLD in rats, and furthermore, the mechanism was explored. PG supplementation significantly increased body weight, serum total cholesterol, triglycerides, and LDL-cholesterol levels in HFD-induced obese rats (<i>p</i> < 0.05). In addition, HFD-induced lipid accumulation and inflammatory response in the liver were also suppressed. Then, the factors contributing to the regulating effect of PG on NAFLD were estimated by using network pharmacology and RNA sequencing. Moreover, western blotting and reverse transcription quantitative polymerase chain reaction (RT-qPCR) suggested that PG treatment obviously downregulated the expression levels of nuclear factor-κB (NF-κB), Toll-like receptor 4 (TLR4), myeloid differentiation primary response protein 88 (MyD88), and inhibitor I kappa B alpha (IκBα), whereas it upregulated the expression of peroxisome proliferator-activated receptor-gamma (PPARγ). Taken together, these findings corroborated the modulatory capacity of PG on NAFLD rats through regulating inflammatory response via PPARγ and TLR4/NF-κB pathway.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144100891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel LC-MS/MS Method for Quantification of Famotidine and Metoprolol and Application to Pharmacokinetic Interaction 新型LC-MS/MS法莫替丁和美托洛尔的定量分析及药动学相互作用研究

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-19 DOI: 10.1002/bmc.70114

Ran Xu, En-Fu Feng, Sun-Jun Yin, Ping Wang, Rui Meng, Xue-Sha Zhang, Jian-Mei Pan, Gong-Hao He

{"title":"Novel LC-MS/MS Method for Quantification of Famotidine and Metoprolol and Application to Pharmacokinetic Interaction","authors":"Ran Xu, En-Fu Feng, Sun-Jun Yin, Ping Wang, Rui Meng, Xue-Sha Zhang, Jian-Mei Pan, Gong-Hao He","doi":"10.1002/bmc.70114","DOIUrl":"https://doi.org/10.1002/bmc.70114","url":null,"abstract":"<div>\u0000 \u0000 <p>A rapid and sensitive LC-MS/MS method was developed and validated to simultaneously determine famotidine (FAM) and metoprolol (MET) in rat plasma and applied to study the pharmacokinetic drug–drug interaction between these two drugs in rats. In this method, D4-famotine (D4-FAM) and D6-metoprolol (D6-MET) were used as the internal standard and methanol protein precipitation method was used for sample preparation. After extraction, the samples were carried on an Agilent Gemini-NX C<sub>18</sub> column and subjected to a gradient elution process using a mixture of methanol and water containing 0.1% formic acid at a flow rate of 0.4 mL/min within 8 min. The monitored transitions were m/z 338.1 → 189.1 for FAM, 268.2 → 116.1 for MET, 342.1 → 190 for D4-FAM, and 274.2 → 122.1 for D6-MET. The analytes had good linearity in the range of 1–200 ng/mL for FAM and 1–400 ng/mL for MET, with the lower limit of quantitation of 1 ng/mL for both drugs. The validated method was verified to meet the determination requirements of biological samples. It was the first time to study the pharmacokinetics interaction between FAM and MET successfully, which would be necessary and beneficial to explore the clinical safety and efficacy of the combination of these two drugs in the treatment of HF.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validated HPLC Method for the Pharmacokinetic Study of Linezolid in Rats 高效液相色谱法研究利奈唑胺在大鼠体内的药动学

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-19 DOI: 10.1002/bmc.70107

Jin-yao Liu, Ying-ying Pang, Piao-piao Wang, Wen-yan Li, Jing Dong

{"title":"Validated HPLC Method for the Pharmacokinetic Study of Linezolid in Rats","authors":"Jin-yao Liu, Ying-ying Pang, Piao-piao Wang, Wen-yan Li, Jing Dong","doi":"10.1002/bmc.70107","DOIUrl":"https://doi.org/10.1002/bmc.70107","url":null,"abstract":"<div>\u0000 \u0000 <p>Linezolid is an oxazolidinone with potent antibacterial activity against multidrug resistant Gram-positive bacteria. This study aimed to develop and validate a sensitive, rapid and reliable high-performance liquid chromatography-photodiode array detector method for determining linezolid concentrations in the plasma and urine and to further characterize the pharmacokinetics of linezolid in rats. Chromatographic separation was performed using a SinoPak BEH C<sub>18</sub> column with isocratic elution (acetonitrile: water, 28:72, v/v). The retention times of linezolid and the internal standard are 3.30 and 5.20 min, respectively. The calibration curve for linezolid was linear over the concentration range of 0.25–50.0 μg/mL in rat plasma and 1.00–100.0 μg/mL in rat urine. Intra- and inter-assay precision values were below 9.00%, with accuracy ranging from 0.67% to 8.34%. Plasma samples were extracted using acetonitrile-mediated protein precipitation, and the recovery was > 93%. Following intragastric (63 mg/kg) and intravenous (25 mg/kg) administration of linezolid to rats, the area under the plasma concentration–time profile from time 0 to infinity were 224.5 and 86.4 μg·h/mL, total clearance were 0.29 and 0.30 L/h/kg, respectively. The cumulative urinary excretion rates of linezolid were 26.2%–27.5% for both administration routes. The presented method can certainly be used for routine analysis of linezolid in plasma and urine.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144085080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Quantification of Methylene Blue and Evaluation of Its Pharmacokinetics in ICR Mice by Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry Using Difluoroacetic Acid” 对“二氟乙酸液相色谱-四极杆飞行时间质谱法测定亚甲基蓝及其在ICR小鼠体内药代动力学评价”的修正

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-16 DOI: 10.1002/bmc.70116

引用次数: 0

Gut Microbiota Combined With Metabolomics to Reveal the Mechanism of Tang Wang Ming Mu Granule in the Treatment of Diabetic Retinopathy in Mice 肠道菌群联合代谢组学揭示汤王明目颗粒治疗小鼠糖尿病视网膜病变的机制

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-16 DOI: 10.1002/bmc.70112

Hong Xing, Yu-Jie Ding, Yuan Zhang, Ning-Ning Li, Shu-Zhen Hou, Er-Wei Liu, Xiao-Peng Chen

{"title":"Gut Microbiota Combined With Metabolomics to Reveal the Mechanism of Tang Wang Ming Mu Granule in the Treatment of Diabetic Retinopathy in Mice","authors":"Hong Xing, Yu-Jie Ding, Yuan Zhang, Ning-Ning Li, Shu-Zhen Hou, Er-Wei Liu, Xiao-Peng Chen","doi":"10.1002/bmc.70112","DOIUrl":"https://doi.org/10.1002/bmc.70112","url":null,"abstract":"<div>\u0000 \u0000 <p>Diabetes retinopathy (DR) is one of the serious complications of diabetes. Clinical practice has proved that Tang Wang Ming Mu Granule (TWMM) can improve symptoms of DR patients. The mechanism of TWMM in treating DR in mice was studied, combining gut microbiota with metabolomics. A high-fat and high-sugar diet combined with streptozotocin (STZ) injection was used to create a mouse model of DR. The C57BL6/J wild-type mice were divided into five groups, including normal control, DR model, TWMM (2.7 and 10.8 g/kg) treatment, and the positive control treatment groups. Based on urine metabolomics and 16S rDNA sequencing of fecal samples, the effects of TWMM on host metabolism and intestinal microbiota were studied. The results showed that TWMM reverses the disordered intestinal flora to normal. In addition, the pathway prediction of intestinal microorganisms was related to the metabolic pathways. Meanwhile, the metabolomics analysis found that the differential metabolites were mainly concentrated in amino acids and their metabolites, carbohydrates, and their metabolites. The Shigellosis pathway attracted attention, and <i>Shigella</i> shows good indication in the treatment. The research provides a method for metabolic disease study with gut microbiota combined with metabolomics and treatment targets and pathways of DR.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Active Ingredients of Hepatoprotective Effect of Raw and Stir-Baked Gardeniae Fructus Based on Spectrum–Effect Relationship Analysis 基于谱效关系分析的生炒栀子保肝活性成分研究

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-16 DOI: 10.1002/bmc.70115

Yangang Cao, Shujing Li, Peiyang Zhang, Hongwei Li, Zhiyou Hao, Xiaoke Zheng, Weisheng Feng

{"title":"Exploring Active Ingredients of Hepatoprotective Effect of Raw and Stir-Baked Gardeniae Fructus Based on Spectrum–Effect Relationship Analysis","authors":"Yangang Cao, Shujing Li, Peiyang Zhang, Hongwei Li, Zhiyou Hao, Xiaoke Zheng, Weisheng Feng","doi":"10.1002/bmc.70115","DOIUrl":"https://doi.org/10.1002/bmc.70115","url":null,"abstract":"<div>\u0000 \u0000 <p>Gardeniae Fructus (GF) has been used as a hepatoprotective medicine; however, the active ingredients of GF against cholestatic liver injury (CLI) remain unclear. This study aims to explore active ingredients of the hepatoprotective effects of raw GF (RGF) and stir-baked GF (SGF) by spectrum–effect relationship analysis. A total of 32 common peaks were recorded in RGF and SGF HPLC fingerprints, and nine of them were structurally characterized. Both RGF and SGF demonstrated hepatoprotective effects in an ANIT-induced CLI rat model. The spectrum–effect relationship analysis results showed that peaks 3 (gardenoside), 4, 5 (jasminoside B), 6 (genipin 1-gentiobioside), 8 (geniposide), 9, 16, 17, 19, and 22 were determined as the potential active ingredients of GF against CLI. Notably, gardenoside, jasminoside B, and genipin 1-gentiobioside exhibited good hepatoprotective effects. The research establishes a research foundation for the future quality control and medicinal application of GF.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-Targeted Metabolomics Analysis of Metabolite Differences in Inocutis tamaricis Under Different Culture Conditions 不同培养条件下柽柳链球菌代谢物差异的非靶向代谢组学分析

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-16 DOI: 10.1002/bmc.70113

Na Zhang, Qi Cui, Xiao-Feng Ma, Kai Huang, Qin Tian, Tian-Li Zhang, Guo-Chao Wu, Shu-De Yang, Xian Hao Cheng, Rui Zhang, Yong-Fei Ming

{"title":"Non-Targeted Metabolomics Analysis of Metabolite Differences in Inocutis tamaricis Under Different Culture Conditions","authors":"Na Zhang, Qi Cui, Xiao-Feng Ma, Kai Huang, Qin Tian, Tian-Li Zhang, Guo-Chao Wu, Shu-De Yang, Xian Hao Cheng, Rui Zhang, Yong-Fei Ming","doi":"10.1002/bmc.70113","DOIUrl":"https://doi.org/10.1002/bmc.70113","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Inocutis tamaricis</i> is a medicinal fungus with significant pharmacological activity. In this study, ultra-high performance liquid chromatography-high resolution mass spectrometry (UPLC/MS) was used to conduct a comprehensive non-targeted metabolomics analysis of the metabolites of wild fruiting body (WF), cultured fruiting body (CF), and liquid cultured mycelium (LCM). Orthogonal partial least square discriminant analysis (OPLS-DA) was used to compare the three culture methods, to describe the regulatory effect of specific culture methods on metabolite distribution and yield of <i>I. tamaricis</i>, and the differential metabolites were enriched and analyzed to identify key metabolic pathways. The results showed significant differences in the types and abundances of active substances produced in different culture methods. There were 35 compounds in common, the WF, CF, and LCM had their unique metabolites, which were 39, 16, and 29 respectively, confirming the significant differences in the metabolites in different cultures, six key metabolic pathways were identified, including unsaturated fatty acid biosynthesis, linoleic acid, and <i>α</i>-linolenic acid metabolism, etc.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comparative Research of the Flavonoid Metabolites From Viscum coloratum in Normal and RA Rats by an Integrated Analytical Strategy

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-05-13 DOI: 10.1002/bmc.70105

Yu-Qing Wang, Wei Guan, Yan-Ying Li, Bo Wen, Zhi-Jiang Chen, Shuang Liu, Yan-Fu Wang, Zhi-Chao Hao, Qing-Shan Chen, Li-li Zhang, Shu Liu, Anam Naseem, Yao-Xin Sui, Si-Tong Liu, Hai-Xue Kuang, Bing-You Yang, Yan Liu

{"title":"A Comparative Research of the Flavonoid Metabolites From Viscum coloratum in Normal and RA Rats by an Integrated Analytical Strategy","authors":"Yu-Qing Wang, Wei Guan, Yan-Ying Li, Bo Wen, Zhi-Jiang Chen, Shuang Liu, Yan-Fu Wang, Zhi-Chao Hao, Qing-Shan Chen, Li-li Zhang, Shu Liu, Anam Naseem, Yao-Xin Sui, Si-Tong Liu, Hai-Xue Kuang, Bing-You Yang, Yan Liu","doi":"10.1002/bmc.70105","DOIUrl":"https://doi.org/10.1002/bmc.70105","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Viscum coloratum</i> (Kom.) Nakai has been demonstrated to be an effective treatment for rheumatoid arthritis (RA), but the pharmacodynamic substances are still unclear. In this study, a four-step strategy integrated nontargeted metabolomics, multivariate statistical analysis, and UNIFI software. An ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) method was employed to characterize 56 flavonoids from <i>V. coloratum</i>, 25 prototypes and 133 metabolites in biological samples of rats following oral administration of <i>V. coloratum</i>. The endogenous interference peaks in plasma, urine, and feces were reduced by 83.79%, 91.60%, and 86.02%, respectively, through the application of nontargeted metabolomics approaches. The distinctions and commonalities in the flavonoid metabolic pathways of <i>V. coloratum</i> under normal and RA conditions were summarized. Phase II metabolism was significantly affected in the RA rats, especially the prototype exposure and its metabolites in plasma and excreted by urine and feces. Utilizing the aforementioned methods, we identified 109 differential metabolites, including 17 RA-specific metabolites. Twenty-two flavonoid prototypes and their metabolites were identified as potential pharmacodynamic substances in plasma. All the information gained from this study will significantly contribute to elucidating the potential biological and pharmacological mechanisms of flavonoids in <i>V. coloratum</i>, thereby opening new avenues for drug development.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0