Exploring the Compatibility Mechanism of Xiaojianzhong Decoction Intervening in Chronic Atrophic Gastritis Rats Based on Serum Pharmacochemistry and Metabolomics

IF 1.8 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2025-07-01 DOI:10.1002/bmc.70159

Wentian Lu, Junjie Guo, Ruoxin Xiang, Yuetao Liu

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Abstract

Xiaojianzhong decoction (XJZ) is a classical traditional Chinese medicine (TCM) formula for treating chronic atrophic gastritis (CAG), but its compatibility mechanism has not been fully elucidated. Traditional pharmacodynamic evaluation, serum pharmacochemistry, and metabolomics techniques were used to link changes in pharmacodynamic substances to changes in the metabolism. The results of serum pharmacochemical analysis showed that the “Jun-drug” affected the metabolism of 3 components in the “Chen-drug,” the absorption of 3 components, and the metabolism of 14 components in the “Zuo-drug”; the “Chen-drug” affected the absorption of 2 components and the metabolism of 12 components in the “Zuo-drug”; and the “Zuo-drug” affected the absorption of 2 components and the metabolism of 4 components in the “Chen-drug.” The analysis of CAG-related metabolites demonstrated that 11 differential metabolites were significantly regulated by “Jun drug,” and 13 differential metabolites were significantly regulated by “Chen drug” and “Zuo drug.” Molecular docking demonstrated that serum components of high correlation exhibited good binding activity to one or more targets of the differential metabolites. This study was helpful to understand the compatibility mechanism of TCM formulas.

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基于血清药物化学和代谢组学研究消健中汤干预慢性萎缩性胃炎大鼠的配伍机制

消建中汤是治疗慢性萎缩性胃炎的经典中药方剂，但其配伍机制尚未完全阐明。传统的药效学评价、血清药物化学和代谢组学技术被用来将药效学物质的变化与代谢的变化联系起来。血清药物化学分析结果显示，“君药”影响了“陈药”中3种成分的代谢，影响了“左药”中3种成分的吸收，影响了“左药”中14种成分的代谢；“陈药”影响“左药”中2种成分的吸收和12种成分的代谢；“左药”影响“陈药”中2种成分的吸收和4种成分的代谢。cag相关代谢物分析显示，“君药”显著调节11种差异代谢物，“陈药”和“左药”显著调节13种差异代谢物。分子对接表明，高相关性的血清成分对差异代谢物的一种或多种靶标具有良好的结合活性。本研究有助于了解中药方剂的配伍机理。

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.