Biomedical Chromatography最新文献_第10页

Specific DNA aptamer–immobilized cryogel membranes as novel bioaffinity supports and their potential for the purification of activated protein C 作为新型生物亲和性支持物的特异性 DNA 配合物固定化冷凝胶膜及其在纯化活化蛋白 C 方面的潜力。

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2024-08-27 DOI: 10.1002/bmc.5995

Nilufer Aliyeva, Semra Akgönüllü, Arzum Erdem, Adil Denizli

{"title":"Specific DNA aptamer–immobilized cryogel membranes as novel bioaffinity supports and their potential for the purification of activated protein C","authors":"Nilufer Aliyeva, Semra Akgönüllü, Arzum Erdem, Adil Denizli","doi":"10.1002/bmc.5995","DOIUrl":"10.1002/bmc.5995","url":null,"abstract":"Activated protein C (APC), a serine protease produced from zymogen protein C (PC), is the key enzyme of the protein C pathway. APC has anticoagulant, anti-inflammatory, and cytoprotective features. APC has recently been shown to significantly reduce coagulation as well as mortality in patients with severe sepsis. Herein, we aimed to develop an affinity support material that allows the purification of plasma APC for the first time. In this research, a novel APC-specific DNA aptamer–based poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) (poly(HEMA-GMA/DNA-Apt)) macroporous cryogel membrane at different molar ratios was prepared using affinity binding method and their potential for purification and identification of APC was investigated. The DNA aptamer–immobilized cryogels were characterized to examine their structural and morphological properties. The effect of pH, initial concentration, temperature, ionic strength difference, and flow rate changes was examined. Selectivity studies were performed in the presence of APC and competitive proteins, and cryogel support materials were shown to have a very high affinity for APC. Adsorption capacity was found to be 89.02 mg/g. Finally, NaCl revealed efficiency for APC desorption and the reuse of cryogels was successfully tested for ten cycles.","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"38 11","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and quantification of the antioxidants in Ginkgo biloba leaf 银杏叶中抗氧化剂的鉴定和定量。

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2024-08-27 DOI: 10.1002/bmc.5980

Chenxiu Hu, Yujing Wang, Yingqian Deng, Jianbiao Yao, Hui Min, Jiqiang Hu, Xiaohui Fan, Shufang Wang

{"title":"Identification and quantification of the antioxidants in Ginkgo biloba leaf","authors":"Chenxiu Hu, Yujing Wang, Yingqian Deng, Jianbiao Yao, Hui Min, Jiqiang Hu, Xiaohui Fan, Shufang Wang","doi":"10.1002/bmc.5980","DOIUrl":"10.1002/bmc.5980","url":null,"abstract":"The antioxidant activity of Ginkgo biloba leaf (GBL) extract is closely related to its efficacy against various diseases; however, the antioxidant activities of the specific constituents of GBL remain unclear. In this study, 194 GBL constituents were identified using ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry, including 97 flavonoids, 37 terpenoids, 29 lignans, 19 carboxylic acids, 5 alkylphenolic acids, 5 alkylphenols, and 2 other compounds. The cleavage rules of the main constituents of GBL were dissected in detail. The 36 GBL constituents with high antioxidant activity were subsequently discovered using the oxygen radical absorbance capacity assay, including 30 flavonoids and six carboxylic acids. Finally, an HPLC analysis method was established to determine the content of the nine major antioxidants in the three batches of GBL. Among them, kaempferol 3-O-β-D-(6″-p-coumaroyl) glucopyranosyl-(1-2)-α-L-rhamnopyranoside, kaempferol-3-O-rutinoside, and rutin exhibited high antioxidant activity and were found in significant amounts in GBL, with concentrations greater than 0.7 mg/g. These results provide an important reference for the development of pharmaceuticals and health products containing GBL.","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"38 11","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mass spectrometry-based metabolomics reveals metabolism of molnupiravir may lead to metabolic disorders and hepatotoxicity 基于质谱的代谢组学揭示了莫仑吡韦的代谢可能导致代谢紊乱和肝毒性。

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2024-08-23 DOI: 10.1002/bmc.5996

Jiahui Chen, Liqiong Chen, Bin Li, Qi Zhao, Yan Cheng, Dongmei Yan, Hongning Liu, Fei Li

{"title":"Mass spectrometry-based metabolomics reveals metabolism of molnupiravir may lead to metabolic disorders and hepatotoxicity","authors":"Jiahui Chen, Liqiong Chen, Bin Li, Qi Zhao, Yan Cheng, Dongmei Yan, Hongning Liu, Fei Li","doi":"10.1002/bmc.5996","DOIUrl":"10.1002/bmc.5996","url":null,"abstract":"Molnupiravir (MO) is a pyrimidine nucleoside anti-SARS-CoV-2 drug. MO treatment could cause mild liver injury. However, the underlying mechanism of MO-induced liver injury and the metabolic pathway of MO in vivo are unclear. In this study, metabolomics analysis and molecular biology methods were used to explore these issues. Through metabolomics analysis, it was found that the homeostasis of pyrimidine, purine, lysophosphatidylcholine (LPC), and amino acids in mice was destroyed after MO treatment. A total of 80 changed metabolites were detected. Among these changed metabolites, 4-ethylphenyl sulfate, dihydrouracil, and LPC 20:0 was related to the elevation of alkaline phosphatase (ALP), interleukin-6 (IL6), and nuclear factor kappa-B (NF-κB). The levels of 4-ethylphenyl sulfate, dihydrouracil, and LPC 20:0 in plasma were positively correlated with their levels in the liver, suggesting that these metabolites were associated with MO-induced liver injury. MO treatment could increase NHC and cytidine levels, activate cytidine deaminase (CDA), and increase LPC levels. CDA and LPC could increase the mRNA expression level of toll-like receptor (TLR). The current study indicated that the elevation of hepatic TLR may be an important reason for MO leading to the liver injury.","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"38 11","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tandem mass tag-based proteomics reveals the antiepileptic mechanism of steroidal saponins from Anemarrhena asphodeloides in Kainic acid induced epileptic rat model 基于串联质量标签的蛋白质组学揭示了凯尼酸诱导的癫痫大鼠模型中天南星甾体皂苷的抗癫痫机制。

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2024-08-22 DOI: 10.1002/bmc.5989

Jian-Jia Zhang, Wei Guan, Yue Wang, Yu-Xuan Wang, Dong-Qi An, Zhi-Chao Hao, Meng-Meng Li, Hai-Xue Kuang, Qing-Shan Chen, Li-Li Zhang, Yan Liu, Bing-You Yang

{"title":"Tandem mass tag-based proteomics reveals the antiepileptic mechanism of steroidal saponins from Anemarrhena asphodeloides in Kainic acid induced epileptic rat model","authors":"Jian-Jia Zhang, Wei Guan, Yue Wang, Yu-Xuan Wang, Dong-Qi An, Zhi-Chao Hao, Meng-Meng Li, Hai-Xue Kuang, Qing-Shan Chen, Li-Li Zhang, Yan Liu, Bing-You Yang","doi":"10.1002/bmc.5989","DOIUrl":"10.1002/bmc.5989","url":null,"abstract":"Epilepsy (EP) is one of the most common neurological diseases in the world. Anemarrhena asphodeloides Bunge. (AA), as a typical heat-cleaning medicine, has been proven to possess the antiepileptic effect in clinical and experimental studies. Anemarrhena asphodeloides steroidal saponins (AAS) are main components. However, the therapeutic effects and underlying mechanisms of AAS against EP are not been fully elucidated. In this study, 63 steroidal saponins were discovered in AAS by UPLC-Q-TOF/MS analysis. Pharmacological and behavioral analysis demonstrated that AAS could significantly lower the Racine classification and reduce the frequency of generalized spike rhythm the rate of tetanic seizures in kainic acid–induced epileptic rats. Hematoxylin and eosin and Nissl staining-indicated AAS could significantly improve hippocampal injury and neuron loss in epileptic rats. TMT proteomic analysis discovered 26 different expressed proteins (DEPs), which were identified as the rescue proteins. After bioinformatic analysis, Heat Shock Protein 90 Alpha Family Class B Member 1 (Hsp90ab1) and Tyrosine 3-Monooxygenase (Ywhab) were screened as key DEPs and verified by western blotting. AAS could significantly inhibited the up-regulation of Hsp90ab1 and Ywhab in EP rats; these two proteins might be the key targets of AAS in treating EP.","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"38 11","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142016245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral solid dosage form using alternate crystalline neratinib maleate anhydrous form: Pharmaceutical, bioequivalent, and clinical perspectives 使用马来酸奈拉替尼无水替代结晶的口服固体制剂：制药、生物等效性和临床角度。

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2024-08-20 DOI: 10.1002/bmc.5988

Congmei Ming, Zhi Liu, Qiong Wang, Ling Liu, Xiaokun Shen

引用次数: 0

Uncovering the material basis and mechanism of Jianwei Xiaoshi tablet against functional dyspepsia using ultra-high-performance liquid chromatography-mass spectrometry and network pharmacology 利用超高效液相色谱-质谱联用技术和网络药理学揭示健胃消食片治疗功能性消化不良的物质基础和机理

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2024-08-20 DOI: 10.1002/bmc.5990

Xiaoxu Cheng, Wanqiao Zhang, Chaodong Huang, Pei Hu, Hongchang Li, Yiguang Li, Yanxia Xiong, Wenjun Liu

{"title":"Uncovering the material basis and mechanism of Jianwei Xiaoshi tablet against functional dyspepsia using ultra-high-performance liquid chromatography-mass spectrometry and network pharmacology","authors":"Xiaoxu Cheng, Wanqiao Zhang, Chaodong Huang, Pei Hu, Hongchang Li, Yiguang Li, Yanxia Xiong, Wenjun Liu","doi":"10.1002/bmc.5990","DOIUrl":"10.1002/bmc.5990","url":null,"abstract":"Functional dyspepsia (FD) is a common digestive disease. Jianwei Xiaoshi (JWXS) tablet is composed of Radix Pseudostellariae (TZS), Pericarpium Citri Reticulatae (CP), Rhizoma Dioscoreae (SY), fired Hordei Fructus Germinatus (CMY) and Crataegi Fructus (SZ). It is a commonly used drug in the treatment of FD in China and has good therapeutic effects. However, there is very little research about the substance basis and action mechanism of JWXS tablet. In this research, ultra-high-performance liquid chromatography-mass spectrometry (UPLC-MS) and network pharmacology were used to explore the substance basis and action mechanism of the JWXS tablet. Finally, 19, 79, 22, 22 and 39 constituents were identified in the extracts of TZS, CP, SY, CMY and SZ, respectively. Based on these findings, a total of 104 ingredients were identified in JWXS tablet and 29 potentially absorbed ingredients were detected in rat plasma. The results of network pharmacology indicated that the inhibition of gastric acid secretion, the regulation of gastrointestinal motility, inflammation and immune response were the key approaches for treating FD with JWXS tablet. The material basis and potential action mechanism of JWXS tablet in treating FD were comprehensively clarified for the first time. This study will improve our understanding of JWXS tablet.","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"38 11","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revealing the optimal traditional processing methods and its protective effects against febrile seizures of Arisaema cum bile 揭示马钱子暨胆汁的最佳传统加工方法及其对热性惊厥的保护作用。

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2024-08-20 DOI: 10.1002/bmc.5977

Meng-Meng Zhang, Xu Wu, Jing Wang, Ting Zou, Su-Rong He, Qiao Zhang, Yi-Jun Song, Chang-Li Wang, Chong-Bo Zhao

{"title":"Revealing the optimal traditional processing methods and its protective effects against febrile seizures of Arisaema cum bile","authors":"Meng-Meng Zhang, Xu Wu, Jing Wang, Ting Zou, Su-Rong He, Qiao Zhang, Yi-Jun Song, Chang-Li Wang, Chong-Bo Zhao","doi":"10.1002/bmc.5977","DOIUrl":"10.1002/bmc.5977","url":null,"abstract":"Arisaema cum bile (known as Dan Nanxing in Chinese, DNX) is a herbal medicine used for treating febrile seizure (FS), which commonly prepared by using Arisaematis Rhizoma and animal bile. This study was designed to explore the optimal processing time of DNX and its potential mechanism on the anti-FS effect. A total of 17 volatile organic compounds (VOCs) were the characteristic ones to distinguish different fermentation stages of DNX by using gas chromatography–ion mobility spectrometry (GC-IMS), such as 2-heptanone monomer, and heptanal monomer. DNX with fermentation for 3 months had an obvious pattern of VOCs with others, which could be regarded as the optimal fermentation time. The Enterococcus and Staphylococcus might be the core bacteria on the production of VOCs. Additionally, DNX (2.8 g/kg, p.o.) reversed hot water bath-induced FSs of rats, as indicated by increased seizure latency and decreased seizure duration time. It also prevented hippocampal neuronal loss, increased GABAAR, and decreased GRIA1 expression. At the genus level, relative abundance of Enterococcus and Akkermansia were enriched after DNX treatment. These findings suggested that fermentation for 3 months might be the optimal process time for DNX, and DNX possess an anti-FS effect through regulating neurotransmitter disorder and gut microbiota.","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"38 11","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142003548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Addressing stability issues of vildagliptin: Method optimization and validation for accurate analysis in human plasma 解决维达列汀的稳定性问题：人血浆中准确分析方法的优化和验证。

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2024-08-20 DOI: 10.1002/bmc.5991

Santosh Tawari, Ujashkumar Shah

{"title":"Addressing stability issues of vildagliptin: Method optimization and validation for accurate analysis in human plasma","authors":"Santosh Tawari, Ujashkumar Shah","doi":"10.1002/bmc.5991","DOIUrl":"10.1002/bmc.5991","url":null,"abstract":"This research paper introduces novel strategies to address the stability issues arising with vildagliptin, marking the first attempt to tackle this challenge comprehensively. The study incorporates malic acid into the human plasma, a crucial step in stabilizing vildagliptin and preventing its degradation. Additionally, optimization of the elution process on a C18 Asentis Express column, fine-tuned with a combination of acetonitrile and ammonium trifluoroacetate 5mM, ensures optimal chromatographic conditions. For detection and quantification, electrospray ionization (ESI) is employed, monitoring multiple reactions for vildagliptin (304.2 → 154.2) and vildagliptin D7 (311.1 → 161.2). Meticulous validation of the method demonstrates high accuracy (97.30%–104.15%) and precision [(0.32%–3.09% coefficient of variance (CV)] for vildagliptin calibration curve standards (CC STD), establishing its sensitivity and reliability in measuring vildagliptin levels. This refined methodology offers numerous advantages, including the elimination of stability concerns, reduced human plasma sample volume (100 μL), exceptional reproducibility, shortened run time (~2.2 min), and a wide concentration range (1.00 to 851.81 ng/mL). These attributes make it exceptionally well-suited for diverse research applications, spanning from extensive sampling in therapeutic drug monitoring units to bioequivalence and bioavailability studies, as well as pharmacokinetic investigations of vildagliptin.","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"38 11","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

P-glycoprotein-mediated herb–drug interaction evaluation between Tenacissoside G and paclitaxel 特纳西索苷 G 与紫杉醇之间 P-糖蛋白介导的草药-药物相互作用评价。

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2024-08-17 DOI: 10.1002/bmc.5984

Jiudong Hu, Yujie Hu, Lingyan Xu, Junjun Chen, Meizhi Shi, Wenhui Wu, Jiao Yang, Yonglong Han

{"title":"P-glycoprotein-mediated herb–drug interaction evaluation between Tenacissoside G and paclitaxel","authors":"Jiudong Hu, Yujie Hu, Lingyan Xu, Junjun Chen, Meizhi Shi, Wenhui Wu, Jiao Yang, Yonglong Han","doi":"10.1002/bmc.5984","DOIUrl":"10.1002/bmc.5984","url":null,"abstract":"P-glycoprotein (P-gp)-mediated herb–drug interactions (HDIs) may impact drug efficacy and safety. Tenacissoside G (Tsd-G), a major active component of Marsdenia tenacissima, exhibits anticancer activity. To analyze the effect of Tsd-G on the pharmacokinetics of paclitaxel (PTX), researchers selected 30 Sprague–Dawley (SD) rats, randomized into a solvent control group, a verapamil positive control group, and 20, 40, and 60 mg/kg Tsd-G groups. After seven consecutive days of intraperitoneal injection of verapamil or Tsd-G, a single dose of 6 mg/kg PTX was injected intravenously. Plasma samples were collected at different time points, and proteins were precipitated using a methanol–acetonitrile solution. An ultrahigh-performance liquid chromatography–tandem mass spectrometry method was developed, with docetaxel as an internal standard, and quantified using positive ion multiple reaction monitoring (MRM) mode. This analytical method's specificity, accuracy, precision, recovery, matrix effect, and sample stability meet the requirements for biological sample determination. After Tsd-G administration in rats, the mean residence time of PTX was significantly prolonged. And Tsd-G can stably bind to P-gp by forming hydrogen bonds and inhibiting the expression of P-gp in rat liver. Although the metabolites of PTX were not detected in this study, the above results still indicate the existence of HDIs between Tsd-G and PTX, and P-gp may be the main target to mediate HDIs.","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"38 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the anticancer potential of certain phenolic and flavonoid components in ginger capsules using colorectal cancer cell lines coupled with quantitative analysis 利用结直肠癌细胞系和定量分析评估生姜胶囊中某些酚类和类黄酮成分的抗癌潜力。

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2024-08-17 DOI: 10.1002/bmc.5993

Khaldun M. Al Azzam, Nadeen Waleed Al-Areer, Rima H. Al Omari, Ibrahim Al-Deeb, Nadia Bounoua, El-Sayed Negim, Ali Al-Samydai, Adam A. Aboalroub, Rana Said

{"title":"Assessment of the anticancer potential of certain phenolic and flavonoid components in ginger capsules using colorectal cancer cell lines coupled with quantitative analysis","authors":"Khaldun M. Al Azzam, Nadeen Waleed Al-Areer, Rima H. Al Omari, Ibrahim Al-Deeb, Nadia Bounoua, El-Sayed Negim, Ali Al-Samydai, Adam A. Aboalroub, Rana Said","doi":"10.1002/bmc.5993","DOIUrl":"10.1002/bmc.5993","url":null,"abstract":"Colorectal cancer (CRC) is the fourth most common cause of malignant tumor death. The development of novel, more effective drugs is desperately needed to treat CRC. Zingiber officinale is believed to possess anticancer properties due to its flavonoids and phenols. Using Soxhlet (SOXT) and maceration (MACR) techniques, the present study aimed to evaluate the amounts of quercetin, gallic acid, rutin, naringin, and caffeic acid in ginger capsules of Z. officinale. High-performance liquid chromatography (HPLC)/ultraviolet was used for separation and quantitation. In vitro toxicity evaluation of ginger capsules on the CRC cell line HT-29 was also conducted to assess the anticancer activity of the supplement. The cell line HT-29 (HTB-38) colorectal adenocarcinoma was utilized for the antiproliferative effect of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide. Ginger herbal supplement extract at dosages of 200 and 100 μg had strong cytotoxic effects (IC50 < 50 μg/mL) on HT-29 CRC cells via MACR. This extract is comparable to the SOXT extract, which has an IC50 of less than 50 μg/mL. The anticancer effect of ginger herbal supplement formulations against CRC lines was investigated, and the results obtained from both the MACR and SOXT extraction procedures were noteworthy. The quercetin content was the highest of all the extracts according to the HPLC data.","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"38 10","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0