Biomedical Chromatography最新文献_第10页

Establishment and Application of Time-Resolved Fluorescence Strip Method for Biotin Detection in Milk Powder 时间分辨荧光条带法检测奶粉中生物素的建立及应用

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-26 DOI: 10.1002/bmc.70057

Jialin Hu, Yongwei Feng, Qi Zhou, Ting Yang, Qianqian Lu, Chuanlai Xu, Lingling Guo

引用次数: 0

Exploring the Mechanism of Action of Honeybran-Fried Cimicifuga Rhizoma in the Treatment of IBS-D Based on Metabolomics and Network Pharmacology 基于代谢组学和网络药理学探讨蜂蜜炒慈母治疗IBS-D的作用机制

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-24 DOI: 10.1002/bmc.70026

Jing Zhu, Yuxuan Zou, Yigeng Wu, Xiaoxia Deng, Yiming Huang, En Yuan, Qi Chen

{"title":"Exploring the Mechanism of Action of Honeybran-Fried Cimicifuga Rhizoma in the Treatment of IBS-D Based on Metabolomics and Network Pharmacology","authors":"Jing Zhu, Yuxuan Zou, Yigeng Wu, Xiaoxia Deng, Yiming Huang, En Yuan, Qi Chen","doi":"10.1002/bmc.70026","DOIUrl":"https://doi.org/10.1002/bmc.70026","url":null,"abstract":"<div>\u0000 \u0000 <p>Honeybran-fried Cimicifuga Rhizoma (HBCR) is often used to treat prolonged diarrhea and prolapse of the anus, uterine prolapse, and gastric ptosis caused by spleen qi deficiency and the inability to elevate qi, and thus the lowering of middle qi. Rats were divided randomly into four groups. Fecal samples of rats in each group were subjected to metabolomics analysis. We identified the chemical components of HBCR using liquid chromatography–tandem mass spectrometry. We predicted the potential active components and key targets of HBCR using network pharmacology to construct a “drug–potential active ingredient–target–disease” network. The key targets screened by network pharmacology and differential metabolites screened by metabolomics analysis were subjected to combined pathway analysis. Pharmacodynamic indices showed that HBCR had a good therapeutic effect upon IBS-D. Metabolomics analysis revealed 26 differential metabolites in the treatment of IBS-D by HBCR. A total of 69 chemical components were identified, and 32 potential active components and 296 key targets were screened. Combination of metabolomics analysis and network pharmacology for joint pathway analysis revealed that the therapeutic effect of HBCR may be affected by the metabolism of linoleic acid, retinol, arachidonic acid, and tryptophan. HBCR had significant therapeutic effects in rats with IBS-D.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Cooperative Strategy of Hippocampus Lipidomics and Anti-Inflammatory Analysis to Evaluate the Antidepressant Effect of Zhi-Zi-Chi Decoction on CUMS Mice 海马脂质组学与抗炎分析联合评价栀子气汤对CUMS小鼠的抗抑郁作用

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-24 DOI: 10.1002/bmc.70058

Chuan Chai, Bo Jin, Tong Xie, Yuhan Cui, Xiaobing Cui, Chenxiao Shan, Sheng Yu, Hongmei Wen

{"title":"A Cooperative Strategy of Hippocampus Lipidomics and Anti-Inflammatory Analysis to Evaluate the Antidepressant Effect of Zhi-Zi-Chi Decoction on CUMS Mice","authors":"Chuan Chai, Bo Jin, Tong Xie, Yuhan Cui, Xiaobing Cui, Chenxiao Shan, Sheng Yu, Hongmei Wen","doi":"10.1002/bmc.70058","DOIUrl":"https://doi.org/10.1002/bmc.70058","url":null,"abstract":"<div>\u0000 \u0000 <p>In this study, Balb/c mice were subjected to chronic unpredictable mild stress (CUMS) and treated with Zhi-zi-chi Decoction (ZZCD). Using a hippocampal lipidomics approach that combined ultra performance liquid chromatography (UPLC)-Q-Exactive Orbitrap MS with multivariate statistical techniques and targeted metabolic pathway analysis, we identified potential lipid metabolites and pathways associated with depression. Meanwhile, anti-inflammatory analyses were conducted in the hippocampus of mice. The chromatograms revealed that most lipids of the same class eluted within the same time period. In the scatter plot, the control and CUMS groups were obviously separated, whereas the ZZCD-treated or fluoxetine-treated groups were positioned between them. In positive and negative ion modes, a comprehensive screening identified 130 differential lipid metabolites, which were classified into 5 groups and 17 types. ZZCD was hypothesized to have a certain call-back efficiency for some differential lipid metabolites. The study identified three target metabolic pathways with certain influence values: glycerophosphate metabolism, linoleic acid metabolism, and α-linolenic acid metabolism. Although ZZCD's inhibitory effect on IL-6 was not significant, it demonstrated good therapeutic effects in reducing central system inflammation associated with IL-1β and TNF-α. The research suggested that the pathogenesis of depression might be closely related to lipid metabolism. ZZCD exhibited antidepressant effects by regulating endogenous lipid metabolism in CUMS mice.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myocardial Lipidomics Revealed Glycerophospholipid and Sphingolipid Metabolism as Therapeutic Targets of Qifu Decoction Against Heart Failure 心肌脂质组学研究发现甘油磷脂和鞘脂代谢是七腑汤抗心衰的治疗靶点。

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-20 DOI: 10.1002/bmc.70063

Xuemei Su, Xin Ding, Junli Liang, Lei Zhang, Yang Zhang, Yan Qiao, Hongrui Ma, Ya Zhang, Yuping Tang, Guangguo Tan

{"title":"Myocardial Lipidomics Revealed Glycerophospholipid and Sphingolipid Metabolism as Therapeutic Targets of Qifu Decoction Against Heart Failure","authors":"Xuemei Su, Xin Ding, Junli Liang, Lei Zhang, Yang Zhang, Yan Qiao, Hongrui Ma, Ya Zhang, Yuping Tang, Guangguo Tan","doi":"10.1002/bmc.70063","DOIUrl":"10.1002/bmc.70063","url":null,"abstract":"<div>\u0000 \u0000 <p>Qifu decoction (QFD) has shown potential benefits in treating heart failure. However, the potential mechanism of QFD remains unclear. In this study, myocardial lipidomics, based on ultra-high-performance liquid chromatography coupled with an electrospray ionization hybrid quadrupole Orbitrap mass spectrometry (UPLC-ESI-Q-Exactive/MS), was employed to identify potential therapeutic targets of QFD for treating heart failure in a mice model induced by ligating the left anterior descending coronary artery. It was found that 47 lipid metabolites were associated with heart failure, of which 35 showed a significant reversal during QFD treatment. The QFD-reversed lipid metabolites were mainly located on phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, and ceramide, which were involved in glycerophospholipid and sphingolipid metabolism. The results of Western blotting analysis revealed that QFD could effectively alleviate heart failure through increasing the levels of lysophosphatidylcholine acyltransferase 1 (LPCAT1) and sphingomyelin synthase 1 (SMS1) and reducing the levels of acid sphingomyelinase (aSMase) and phospholipase A2 (PLA2) to regulate the metabolic disorders of glycerophospholipid and sphingolipid metabolism. All these results could be concluded that glycerophospholipid and sphingolipid metabolism were the two crucial target pathways for QFD against heart failure, which laid the theoretical groundwork for its clinical application.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the Molecular Mechanism of XiaoXianXiong Decoction in the Treatment of Atherosclerosis Based on UHPLC-Q Exactive Focus MS/MS, Network Pharmacology, and Experimental Validation 基于UHPLC-Q焦点质谱联用、网络药理学及实验验证的消仙熊汤治疗动脉粥样硬化分子机制研究

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-19 DOI: 10.1002/bmc.70062

Hou Yafang, Lu Chenxi, Zhang Haoran, Liu Yuan, Ren Feifei, Du Xia, Chen Zhiyong

{"title":"Study on the Molecular Mechanism of XiaoXianXiong Decoction in the Treatment of Atherosclerosis Based on UHPLC-Q Exactive Focus MS/MS, Network Pharmacology, and Experimental Validation","authors":"Hou Yafang, Lu Chenxi, Zhang Haoran, Liu Yuan, Ren Feifei, Du Xia, Chen Zhiyong","doi":"10.1002/bmc.70062","DOIUrl":"10.1002/bmc.70062","url":null,"abstract":"<div>\u0000 \u0000 <p>Atherosclerosis (AS), a leading pathological basis of severe cardiovascular diseases, poses a significant threat to human health. XiaoXianXiong Decoction (XXXD), a classical traditional Chinese medicine (TCM) prescription, has demonstrated promising effects in the treatment of AS. To investigate the underlying mechanism of XXXD in treatment with AS, we used UHPLC-Q Focus MS/MS, network pharmacology and in vivo validation methods. The results showed that 59 chemical components of XXXD were identified. Network pharmacology showed that 11 key compounds, 10 key targets and five key signaling pathways involved in the therapeutic effects of XXXD on AS. Experimental verification confirmed that XXXD significantly improved dyslipidemia, lipid accumulation and pathological changes in the aorta during AS. These effects were linked to the inhibition of the PI3K/AKT signaling pathway, down-regulation of PI3K, HRAS, EGF, CREB and up-regulation of NOS3 expression. This work may provide a theoretical basis for further research on the molecular mechanisms for XXXD in AS treatment.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying of Anticoagulant Ingredients From Moutan Cortex Based on Spectrum-Effect Relationship Analysis Combined With GRA, PLS, and SVM Algorithms 结合GRA、PLS和SVM算法的光谱效应关系分析鉴定牡丹皮抗凝血成分

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-17 DOI: 10.1002/bmc.70060

Weijie Pan, Qianru Chen, Menghua Wu, Yue Sun, Ming Li, Shumei Wang, Xingyang Xue, Jiang Meng

{"title":"Identifying of Anticoagulant Ingredients From Moutan Cortex Based on Spectrum-Effect Relationship Analysis Combined With GRA, PLS, and SVM Algorithms","authors":"Weijie Pan, Qianru Chen, Menghua Wu, Yue Sun, Ming Li, Shumei Wang, Xingyang Xue, Jiang Meng","doi":"10.1002/bmc.70060","DOIUrl":"https://doi.org/10.1002/bmc.70060","url":null,"abstract":"<div>\u0000 \u0000 <p>Moutan Cortex (MC) is a renowned Chinese medicine used for promoting blood circulation and removing blood stasis. However, the active ingredients are unclear. This study aimed to identify and validate the active ingredients of MC. UPLC fingerprints of 23 batches of MC from various origins were analyzed. The activating blood efficacy of MC was assessed by evaluating the inhibitory effects on thrombin and factor Xa (FXa) using the chromogenic substrate method. Active ingredients were identified through spectrum-effect relationship analysis using gray relation analysis (GRA), partial least squares (PLS), and support vector machine (SVM) algorithms. Consequently, five components were identified as potential active ingredients: mudanpioside H, oxypaeoniflorin, 1,2,3,4,6-penta-O-galloyl-β-d-glucose (PGG), benzoyloxypaeoniflorin, and suffruticoside A/B/C/D. The pharmacological activities of these five active ingredients were further confirmed by measuring their thrombin inhibition ability and antithrombotic effects in zebrafish, and their interactions with thrombin and FXa were examined using molecular docking technology. Oxypaeoniflorin, benzoyloxypaeoniflorin, and PGG demonstrated significant efficacy in promoting blood circulation and resolving blood stasis, as well as strong binding affinities. This study provides a biochemical foundation for the anticoagulant effects of MC and offers valuable insights for quality control and the development of novel anticoagulant ingredients drugs.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Performance Liquid Chromatography–Ultraviolet Assay for the Determination of Pirtobrutinib Levels in a Patient With Mantle Cell Lymphoma 高效液相色谱-紫外法测定套细胞淋巴瘤患者吡托鲁替尼水平

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-17 DOI: 10.1002/bmc.70061

Yoshito Gando, Takeo Yasu, Mari Shimoda, Masao Tukada

{"title":"High-Performance Liquid Chromatography–Ultraviolet Assay for the Determination of Pirtobrutinib Levels in a Patient With Mantle Cell Lymphoma","authors":"Yoshito Gando, Takeo Yasu, Mari Shimoda, Masao Tukada","doi":"10.1002/bmc.70061","DOIUrl":"https://doi.org/10.1002/bmc.70061","url":null,"abstract":"<div>\u0000 \u0000 <p>Pirtobrutinib is a Bruton's tyrosine kinase inhibitor used to treat mantle cell lymphoma and chronic lymphocytic leukemia. Pirtobrutinib has a steady-state trough concentration of > 825 ng/mL, corresponding to a 90% inhibitory concentration of Bruton's tyrosine kinase. Therefore, maintaining stable trough concentrations of pirtobrutinib is clinically important; however, no methods of monitoring pirtobrutinib levels have been developed. In this study, our aim was to develop a method to determine pirtobrutinib levels in human plasma and validate it for therapeutic drug monitoring. Pirtobrutinib and ibrutinib (internal standard) were separated on a reversed-phase column using a mobile phase comprising 0.5% KH<sub>2</sub>PO<sub>4</sub> (pH 4.5) and acetonitrile (52:48, v/v) at a flow rate of 1.0 mL/min. Ultraviolet detection was performed at 234 nm. Calibration curves for pirtobrutinib were linear (<i>r</i><sup>2</sup> = 0.9998) in the range of 0.25–10 μg/mL. The intraday and interday validation coefficients were 0.72%–2.86% and 1.29%–3.22%, respectively. This study is the first one to develop and validate a method for quantifying pirtobrutinib in human plasma. These findings may support the widespread application of therapeutic drug monitoring for pirtobrutinib.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolite Identification of Huangqi Guizhi Wuwu Granules in Rat Urine and Feces Based on Ultra–High-Performance Liquid Chromatography With Quadrupole Time-of-Flight Mass Spectrometry 基于超高效液相色谱-四极杆飞行时间质谱法鉴定大鼠尿液和粪便中黄芪桂枝五物颗粒代谢物

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-17 DOI: 10.1002/bmc.70059

Yang Liu, Yan Yang, Xing Wang, Shengyu Ge, Lele Li, Bo Feng, Lili Jin, Jiao Guan, Heyun Zhu

{"title":"Metabolite Identification of Huangqi Guizhi Wuwu Granules in Rat Urine and Feces Based on Ultra–High-Performance Liquid Chromatography With Quadrupole Time-of-Flight Mass Spectrometry","authors":"Yang Liu, Yan Yang, Xing Wang, Shengyu Ge, Lele Li, Bo Feng, Lili Jin, Jiao Guan, Heyun Zhu","doi":"10.1002/bmc.70059","DOIUrl":"https://doi.org/10.1002/bmc.70059","url":null,"abstract":"<div>\u0000 \u0000 <p>Huangqi Guizhi Wuwu Decoction (HGWD), a famous Chinese medicine prescription, has been widely used in the treatment of diabetic peripheral neuropathy and scleroderma in China. Considering that the decoction was not easy to store and carry, our research group has converted HGWD into Huangqi Guizhi Wuwu Granules (HGWG) in the early stage. However, the in vivo metabolism profile of HGWG was still unclear. In this study, an ultra–high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) method was established to identify prototypes and metabolites in urine and feces of rats after oral administration of HGWG. A total of 100 compounds were identified in urine and feces samples. In the urine samples, 28 prototype compounds and 41 related metabolites were identified. In the feces samples, 39 prototype compounds and 12 related metabolites were identified. Monoterpenoid glucosides, flavonoids, organic acids, gingerols, and terpenes were the main prototype compounds in both rat urine and feces. Flavonoid-related metabolites, organic acid-related metabolites, and gingerol-related metabolites were the major metabolites in rat urine, and flavonoid-related metabolites were the major metabolites in rat feces. This study can provide a theoretical basis for the drug metabolism and new drug development research of HGWG.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on the Anti-Inflammatory Effect of Gentiana scabra Bunge Extract and Its Mechanism Using Zebrafish and RAW264.7 Cell Models 利用斑马鱼和RAW264.7细胞模型研究龙胆提取物的抗炎作用及其机制

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-17 DOI: 10.1002/bmc.70050

Yang Yu, Xufeng Jiang, Ruirui Li, Guanggang Xiang, Yang Zhang

{"title":"Study on the Anti-Inflammatory Effect of Gentiana scabra Bunge Extract and Its Mechanism Using Zebrafish and RAW264.7 Cell Models","authors":"Yang Yu, Xufeng Jiang, Ruirui Li, Guanggang Xiang, Yang Zhang","doi":"10.1002/bmc.70050","DOIUrl":"https://doi.org/10.1002/bmc.70050","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Gentiana scabra</i> Bunge (<i>Gentian</i>) is a traditional medicinal plant valued for its anti-inflammatory and analgesic effects, with historical use in treating atopic dermatitis. Despite its therapeutic reputation, a comprehensive scientific analysis of its constituents is lacking. This study systematically evaluates the anti-inflammatory effects of <i>Gentian</i> extract and explores its molecular mechanisms. We characterized the chemical profile of <i>Gentian</i> extracts using HPLC and assessed their anti-inflammatory activity in zebrafish and cellular models. <i>Gentian</i> extract significantly reduced inflammation, as shown by decreased neutrophil migration in response to sodium lauryl sulfate (SLS), reduced tail wagging in zebrafish embryos, and alleviated lipopolysaccharide (LPS)-induced edema. It also lowered reactive oxygen species (ROS) and malondialdehyde (MDA) levels, indicating antioxidant properties, and downregulated pro-inflammatory cytokines and genes. In LPS-stimulated RAW264.7 cells, the extract upregulated IκBα and reduced p65 and STAT3 phosphorylation, inhibiting NF-κB and JAK–STAT pathways. This study is the first to systematically evaluate the anti-inflammatory mechanisms of <i>Gentian</i> extract in zebrafish and RAW264.7 cell models, enhancing its understanding and providing a scientific basis for its application in anti-inflammatory products.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing Therapeutic Drug Monitoring for Oral Targeted Anticancer Drugs: From Hospital-Based Towards Home-Sampling 推进口服靶向抗癌药物的治疗药物监测：从医院到家庭抽样

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-14 DOI: 10.1002/bmc.70056

Marinda Meertens, Hilde Rosing, Neeltje Steeghs, Jos H. Beijnen, Alwin D. R. Huitema

{"title":"Advancing Therapeutic Drug Monitoring for Oral Targeted Anticancer Drugs: From Hospital-Based Towards Home-Sampling","authors":"Marinda Meertens, Hilde Rosing, Neeltje Steeghs, Jos H. Beijnen, Alwin D. R. Huitema","doi":"10.1002/bmc.70056","DOIUrl":"https://doi.org/10.1002/bmc.70056","url":null,"abstract":"<p>Home-sampling for therapeutic drug monitoring (TDM) for oral targeted anticancer drugs offers a promising alternative to traditional hospital-based sampling methods, though it presents challenges. This review aims to summarize the state-of-the-art of home-sampling methods for TDM and evaluates the analytical and clinical validation challenges. A comprehensive search was conducted across Embase, Medline, and Scopus. Eligible articles described analytical and/or clinical validation of home-sampling methods for oral targeted anticancer drugs. ASReview was used to process unique references and to identify relevant studies. Of the 39 included articles, 32 detailed on analytical validation experiments, while 27 covered clinical validation experiments. Dried blood spot and volumetric absorptive microsampling were the primary sampling methods. Key challenges were ensuring robust sample collection, sample pretreatment, hematocrit effects, and sample stability, which were generally thoroughly investigated. Clinical validation yielded promising results for most analytes, although external validation remains crucial for confirming reliability. Home-sampling methods for TDM of oral targeted anticancer drugs show promising results for clinical implementation. Methods for well-studied drugs may be clinically implemented immediately, while others require further external validation. Future research should address device-specific challenges and assess patient feasibility to facilitate the routine use of home-sampling in clinical practice.</p>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bmc.70056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0