Biomedical Chromatography最新文献_第9页

Protective Effects and Mechanisms of Active Fractions of Ixeris sonchifolia on Rats With Toxic Heat and Blood Stasis Syndrome Revealed by Pharmacodynamics and Metabonomics 从药效学和代谢组学的角度探讨红叶黄有效部位对中热血瘀证大鼠的保护作用及其机制

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-12 DOI: 10.1002/bmc.70042

Zhaowei Liu, Yuanyuan Zhao, Yiran Ren, Yifei Liu, Zhenqing Liu, Jingxuan Zhang, Ying Liu

{"title":"Protective Effects and Mechanisms of Active Fractions of Ixeris sonchifolia on Rats With Toxic Heat and Blood Stasis Syndrome Revealed by Pharmacodynamics and Metabonomics","authors":"Zhaowei Liu, Yuanyuan Zhao, Yiran Ren, Yifei Liu, Zhenqing Liu, Jingxuan Zhang, Ying Liu","doi":"10.1002/bmc.70042","DOIUrl":"https://doi.org/10.1002/bmc.70042","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Ixeris sonchifolia</i> (IS) has been demonstrated to have beneficial effects on clearing heat and detoxifying, promoting blood circulation and removing blood stasis. However, the protective effects of active fractions and the underlying mechanisms of IS against toxic heat and blood stasis syndrome (THBSS) remain unclear. This study aimed to investigate this. In this study, the protective effects of 30%, 50%, and 70% ethanol fractions of IS on rats with THBSS were evaluated by observing myocardial pathology, analyzing blood rheology and blood routine indices, and the general conditions of the rats. Comprehensive metabolomic analysis of plasma and myocardium samples was conducted using ultra-high-performance liquid chromatography linear ion trap quadrupole orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap MS/MS). The results showed that IS-30% had a significant protective effect on rats with THBSS, and it could play a protective role by modulating 35 differential metabolites associated with key metabolic pathways, including glycerophospholipid metabolism, sphingosine metabolism, alpha-linolenic acid metabolism, and nicotinate and nicotinamide metabolism. Analysis of different metabolites and pathways showed that they are closely associated with inflammatory responses; therefore, it is hypothesized that IS-30% may protect against THBSS by exerting an anti-inflammatory effect. This study provides valuable data supporting the druggability of IS-30% and offers new insights into its potential therapeutic applications.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stability-Indicating RP-HPLC Method Development and Validation for Determination of Impurities in Loperamide Hydrochloride Capsules Dosage Form 稳定性指示反相高效液相色谱法测定盐酸洛哌丁胺胶囊剂型中杂质的方法建立及验证

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-12 DOI: 10.1002/bmc.70027

Sreenivas Pippalla, Vaishnavi Chintala

{"title":"Stability-Indicating RP-HPLC Method Development and Validation for Determination of Impurities in Loperamide Hydrochloride Capsules Dosage Form","authors":"Sreenivas Pippalla, Vaishnavi Chintala","doi":"10.1002/bmc.70027","DOIUrl":"https://doi.org/10.1002/bmc.70027","url":null,"abstract":"<div>\u0000 \u0000 <p>A quality by design (QbD)-based high-resolution, stability-indicating high-performance liquid chromatography (HPLC) method was developed for determining impurities in loperamide hydrochloride (LPH) tablet dosage forms. Using this method, eight known impurities were qualified, and three degradants were quantified with excellent peak resolution. Mobile Phase-A consisted of 0.05-M tetrabutylammonium hydrogen phosphate buffer and acetonitrile (80:20, v/v), while Mobile Phase-B contained the same buffer and acetonitrile (20:80, v/v). The gradient program was as follows: 0 min, MP-A 95%, MP-B 5%; 15 min, MP-A 30%, MP-B 70%; 17 min, MP-A 30%, MP-B 70%; 19 min, MP-A 95%, MP-B 5%; and 24 min, MP-A 95%, MP-B 5%. Chromatographic separation was performed using a column zodiac C18 (100 mm × 4.6 mm, 3.0 μm) with a flow rate of 1.5 mL/min, detection at 220 nm, an injection volume of 10 μL, and a column temperature of 35°C.</p>\u0000 <p>Stress studies revealed LPH's sensitivity to acidic, oxidative, and thermal conditions. Under all forced degradation conditions, the purity angle was found to be less than the purity threshold, demonstrating the robustness of the method. Validation confirmed its suitability for quality analysis and stability studies in routine manufacturing processes.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on Rapid Detection Technology of Illegal Chemical Compounds Added to External Poultices 外用药膏中非法化合物快速检测技术研究

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-10 DOI: 10.1002/bmc.70053

Xiaoan Li, Yaning Cui, Li Liu, Longjiang Guo, Yudong Liu, Xuegao Song, Na Song, Jianzhong Jia, Zhuanping Zhang

{"title":"Study on Rapid Detection Technology of Illegal Chemical Compounds Added to External Poultices","authors":"Xiaoan Li, Yaning Cui, Li Liu, Longjiang Guo, Yudong Liu, Xuegao Song, Na Song, Jianzhong Jia, Zhuanping Zhang","doi":"10.1002/bmc.70053","DOIUrl":"https://doi.org/10.1002/bmc.70053","url":null,"abstract":"<div>\u0000 \u0000 <p>Topical poultices and applied medical devices that can relieve a variety of cervical vertebrae and lumbar vertebrae pain may have illegal additives of pharmaceutical drugs or analogues have additional health risks, but customers may not be aware of what they are using. National Medical Products Administration reviewed and approved “Supplementary test method for identification and content determination of 17 chemicals in affixed medical devices” drafted by Shandong Provincial Institute of Medical Devices and Drug Packaging Inspection in January 2022, but the test method is simple in sample processing and difficult to extract the chemical components in the paste that may cause the phenomenon of missing detection of most illegal added chemical drugs. In this work, the sample handling and extraction methods have been improved and optimized, the content of illegal added chemicals in 47 batches of affixed medical devices was determined by HPLC-MS/MS method and was detected in 37 batches, and the non-steroidal anti-inflammatory drug nimesulide and aspirin were detected in most samples. It shows that there are illegal chemical drugs added in the medical devices and many people may take several different supplements daily and encounter quite high levels of combined exposure to toxic compounds.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pioneering Human Plasma Detection of Haloperidol With 5-pg/mL Sensitivity via Refined Sample Preparation and Advanced LC–MS/MS 采用精制样品制备和先进LC-MS /MS技术，开创性地检测人血浆氟哌啶醇，灵敏度为5 pg/mL

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-10 DOI: 10.1002/bmc.70048

Santosh Tawari, Ujashkumar Shah

{"title":"Pioneering Human Plasma Detection of Haloperidol With 5-pg/mL Sensitivity via Refined Sample Preparation and Advanced LC–MS/MS","authors":"Santosh Tawari, Ujashkumar Shah","doi":"10.1002/bmc.70048","DOIUrl":"https://doi.org/10.1002/bmc.70048","url":null,"abstract":"<div>\u0000 \u0000 <p>The present study develops, refines, and validates an LC–MS/MS technique to detect haloperidol in human plasma with outstanding sensitivity (5 pg/mL), selectivity, specificity, fast analysis time, and low sample volume to enhance mental treatment regimens. Haloperidol and haloperidol D4 were tested on a Kromasil C18 stationary phase with a 35:65 ratio of 10-mM ammonium trifluoroacetate buffer to acetonitrile. A Strata-X PRO cartridge extracted the analyte and IS with improved sample extraction. ESI with multiple reaction monitoring measured haloperidol (Q1/Q3: 376.1/165.0) and D4 (Q1/Q3: 380.1/169.0). This method has high sensitivity (5.03 pg/mL), extraction efficiency (> 95%), rapid analysis (3 min), and a small sample volume (100 μL). The correction coefficient (<i>r</i><sup>2</sup>) > 0.980 linearized the process from 5.03 to 6020.75 pg/mL. Nominal accuracy was 95.40%–102.52%, while intraday precision (% CV) was 0.92%–5.33%. Additionally, interday accuracy ranged from 95.40% to 102.66% and precision from 2.05% to 5.73%. This bioanalytical method for haloperidol detection in human plasma shows great sensitivity, selectivity, and linearity with an LLOQ of 5.03 pg/mL. It improves pharmacokinetics, bioequivalence, and scale-up bioavailability. Being precise, stable, and adaptable are all advantages in clinical and therapeutic monitoring.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Plasma Protein Binding Determination of Lefamulin by Equilibrium Dialysis and High-Performance Liquid Chromatography Coupled With Evaporative Light Scattering Detector 平衡透析-高效液相色谱-蒸发光散射法测定Lefamulin的体外血浆蛋白结合

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-10 DOI: 10.1002/bmc.70052

Lin Xin, Hong-Liang Xiao, Mei-Di Li, Yi-Kuan Ji, Wen-Zi Liu, Xiao-Ping Liao, Yang Yu, Dong-Hao Zhao

引用次数: 0

A Novel Derivatization-Free Approach for Analysis of (+)2-Aminobutanol and Ethambutol Hydrochloride in Tablets Preparation Using RP-HPLC-NQAD 反相高效液相色谱- nqad分析片剂中（+）2-氨基丁醇和盐酸乙胺丁醇的新方法

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-10 DOI: 10.1002/bmc.70049

Hao Ruan, Ying Luo, Qian Yang, Chao Liu, Yunfeng Shi, Minglong Ye, Liya Hong, Weike Su

{"title":"A Novel Derivatization-Free Approach for Analysis of (+)2-Aminobutanol and Ethambutol Hydrochloride in Tablets Preparation Using RP-HPLC-NQAD","authors":"Hao Ruan, Ying Luo, Qian Yang, Chao Liu, Yunfeng Shi, Minglong Ye, Liya Hong, Weike Su","doi":"10.1002/bmc.70049","DOIUrl":"https://doi.org/10.1002/bmc.70049","url":null,"abstract":"<div>\u0000 \u0000 <p>A novel and rapid RP-HPLC coupled with nano quantity analyte detector (NQAD) method was developed for determination of ethambutol hydrochloride and (+)2-aminobutanol in drug products. NQAD is an aerosol-based detector that can be used for the direct detection of the substance lacking ultraviolet chromophores. The two highly polar analytes were separated on a C<sub>18</sub> column with mobile phase consists of trifluoroacetic acid solution and methanol and detected with NQAD. A thorough investigation was conducted into the experimental and instrumental parameters, including the composition and ratio of mobile phase, the flow-rate of mobile phase, and nebulizer and evaporator temperature, to achieve the highest sensitivity of analytes. The method was validated as per ICH guidelines and proved to be specific, accurate, precise, linear, sensitive, and robust. The method showed adequate performance, with excellent sensitivity for limits of detection (LODs) of 5.6 and 9.8 ng for ethambutol and (+)2-aminobutanol (Impurity I), respectively. Good linearity was obtained for ethambutol and Impurity I in their concentration range both with an <i>R</i><sup>2</sup> value of 0.999. Overall, the method established in this study, utilizing a new detection technique, proved to be accurate and rapid and is applicable for directly detecting Impurity I and ethambutol in quality control.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of Anti-Biofilm Compounds From Paeoniae Radix Alba Based on Oral Biofilm Biochromatography 基于口腔生物膜生物色谱法筛选白芍抗生物膜化合物

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-09 DOI: 10.1002/bmc.70019

Xin-Ming Liu, Yi Yu, Heng Jiang, Yu-Fei Wang, Yan Gao, Lei Xiao, Ming Liang, Jin Qi

{"title":"Screening of Anti-Biofilm Compounds From Paeoniae Radix Alba Based on Oral Biofilm Biochromatography","authors":"Xin-Ming Liu, Yi Yu, Heng Jiang, Yu-Fei Wang, Yan Gao, Lei Xiao, Ming Liang, Jin Qi","doi":"10.1002/bmc.70019","DOIUrl":"https://doi.org/10.1002/bmc.70019","url":null,"abstract":"<div>\u0000 \u0000 <p>Oral biofilms, which are known as dental plaque, are the reason for a wide range of oral and systemic diseases, which contribute to serious health risks. Paeoniae Radix Alba (PRA) is traditionally used as a folk medicine with anti-inflammatory, cardioprotective, and hepatoprotective properties. PRA is currently used in a variety of therapeutic approaches for oral diseases. Nevertheless, its inhibitory effect on oral biofilm formation and the basis for its efficacy have not been clarified. This study intended to screen the potential compounds in PRA that inhibit oral biofilm formation using biochromatography. Two biofilm models based on <i>S. mutans</i> were used to determine the inhibitory effect of PRA on biofilm formation. The extraction of PRA was divided into fractions with different polarity, the active fraction screened, and an HPLC profile constructed for the active fraction. Three potential compounds were screened using targeted oral biofilm extraction, and subsequent validation of the efficacy indicated that albiflorin is the main compound in PRA exerting anti-biofilm activity. Our results have revealed the pharmacological substance basis of PRA in inhibiting the formation of oral biofilm and provide a reference for the further use of PRA in the development of oral health products.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Quantification of Caffeic and Ferulic Acids by HPLC-UV in Nanoparticles: Degradation Profiling and Computational Peroxidation Analysis 用高效液相色谱-紫外同时定量纳米颗粒中的咖啡酸和阿魏酸：降解谱和计算过氧化分析

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-06 DOI: 10.1002/bmc.70051

Igor Roesch Felix, Gabriela Wink Stringhini, Beatriz Miguel Giovanelli, Silvia Leticia Baldissera, Camila Reck Rampelotto, Tielle Moraes de Almeida, Clarissa Piccinin Frizzo, Alisson Vasques Paz, Mariana Zancan Tonel, Scheila Rezende Schaffazick, Cristiane de Bona da Silva

{"title":"Simultaneous Quantification of Caffeic and Ferulic Acids by HPLC-UV in Nanoparticles: Degradation Profiling and Computational Peroxidation Analysis","authors":"Igor Roesch Felix, Gabriela Wink Stringhini, Beatriz Miguel Giovanelli, Silvia Leticia Baldissera, Camila Reck Rampelotto, Tielle Moraes de Almeida, Clarissa Piccinin Frizzo, Alisson Vasques Paz, Mariana Zancan Tonel, Scheila Rezende Schaffazick, Cristiane de Bona da Silva","doi":"10.1002/bmc.70051","DOIUrl":"https://doi.org/10.1002/bmc.70051","url":null,"abstract":"<div>\u0000 \u0000 <p>Phenolic compounds such as ferulic acid (FA) and caffeic acid (CA) are renowned for their antioxidant and anti-inflammatory properties. However, their physicochemical instability limits broader therapeutic applications. This study aimed to develop an analytical methodology for the simultaneous quantification of these compounds in nanoparticles using liquid chromatography. The suspensions exhibited favorable characteristics, including a particle size of 117 nm, encapsulation efficiency above 60%, zeta potential of 14 mV, and adequate bioactive content. Notably, the system enhanced their photostability under UV radiation. The HPLC-UV method employed a C18 column with a mobile phase of acidic water (pH 2.6), methanol, and acetonitrile (64:18:18, v/v/v) at 1 mL/min, with detection at 223 nm, achieving fast and efficient separation. The method was linear, specific, precise, accurate, and robust, in accordance with Brazilian and ICH guidelines. Additionally, the method was stability-indicating, identifying isomeric degradation products, which represent the most common degradation reaction for these compounds. Computational analysis using density functional theory (DFT) further explored the stability of CA and FA. It revealed a strong interaction between the carboxyl group and hydrogen peroxide, suggesting reduced susceptibility to oxidation. These findings provide a foundation for designing stable formulations and enhancing the understanding of antioxidant mechanisms in phenolic compounds.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prunus mume Alleviates Hyperuricemic Renal Injury: Insights From Network Pharmacology and Experimental Models 李梅减轻高尿酸血症肾损伤：网络药理学和实验模型的见解

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-05 DOI: 10.1002/bmc.70035

ShaoJun Zheng, Sheng Li, XiaoHan Diao, NaiDong Chen

{"title":"Prunus mume Alleviates Hyperuricemic Renal Injury: Insights From Network Pharmacology and Experimental Models","authors":"ShaoJun Zheng, Sheng Li, XiaoHan Diao, NaiDong Chen","doi":"10.1002/bmc.70035","DOIUrl":"https://doi.org/10.1002/bmc.70035","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Prunus mume</i> (PM), the dried flower bud of a Rosaceae plant, has a long history of use for its liver-soothing, depression-relieving, and appetite-stimulating effects. Recently, PM has gained attention for its anti-inflammatory, antioxidant, and uric acid-lowering properties. The chemical composition of PM was analyzed using network pharmacology and liquid chromatography–mass spectrometry (LC-MS). The therapeutic potential of PM for hyperuricemia-induced kidney damage was evaluated in a quail model. Antioxidant activity in an HK-2 cell model of hyperuricemia was assessed by measuring the levels of MDA, SOD, and GSH. Additionally, the anti-inflammatory potential was examined using ELISA to measure TNF-α and IL-6 levels. Western blotting was employed to study the effects on URAT1, GLUT9, and the PI3K/AKT pathway. LC-MS identified 284 compounds in PM, with 35 predicted active ingredients. The quail model demonstrated PM's protective effects on the kidneys under hyperuricemic conditions. In vitro, PM reduced oxidative stress and lowered TNF-α and IL-6 levels. It also modulated URAT1 and GLUT9 expression and influenced the PI3K/AKT pathway. PM shows promise in protecting kidneys from hyperuricemia-induced damage, likely through its anti-inflammatory and antioxidant activities, as well as the regulation of urate transport proteins and the PI3K/AKT pathway.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143554719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipidomic Analysis of Serum Lipid Profiles in Idiopathic Central Precocious Puberty and the Potential Regulatory Role of GnRHa in Lipid Metabolism 特发性中枢性性早熟的血脂组学分析及GnRHa在脂质代谢中的潜在调节作用

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-05 DOI: 10.1002/bmc.70029

Li-Xia Yang, Hui Chen, Yan-Hua Jiang, Hui Li, Ling-Ling Yang, Zhao Wang, Hua Shao, Jinjun Shan, Shouchuan Wang, Li-Li Lin, Ling Xu, Jin Ye

{"title":"Lipidomic Analysis of Serum Lipid Profiles in Idiopathic Central Precocious Puberty and the Potential Regulatory Role of GnRHa in Lipid Metabolism","authors":"Li-Xia Yang, Hui Chen, Yan-Hua Jiang, Hui Li, Ling-Ling Yang, Zhao Wang, Hua Shao, Jinjun Shan, Shouchuan Wang, Li-Li Lin, Ling Xu, Jin Ye","doi":"10.1002/bmc.70029","DOIUrl":"https://doi.org/10.1002/bmc.70029","url":null,"abstract":"<div>\u0000 \u0000 <p>Idiopathic central precocious puberty (ICPP) is an endocrine disorder increasingly observed in children, commonly treated with gonadotropin-releasing hormone agonists (GnRHa). However, the serum lipid profiles in girls with ICPP and the potential regulatory effects of GnRHa in lipid metabolism remain unclear. This research analyzed lipidomic profiles of serum samples from girls younger than 10 years of age, including 37 patients with ICPP, 32 ICPP patients treated with GnRHa, and 30 age-matched healthy controls. Using UHPLC-Q-Exactive Orbitrap/MS, we identified a total of 898 differential lipids in both positive and negative modes, covering classes such as phosphatidylcholine (PC, including EtherPC), acylcarnitine (CAR), triglyceride (TG), N-acyl ethanolamines (NAEs), diacylglycerol (DG), sphingomyelin (SM), phosphatidylethanolamine (PE), ceramide (Cer), and fatty acids (FAs). In the ICPP cohort, 105 lipids exhibited significant differences compared with controls, with increased levels of CAR, SM, PC, PE, TG, and FA (<i>p</i> < 0.05), and notably decreased NAE levels (<i>p</i> < 0.05). Specifically, NAE 20:1 (AUC: 1.0), NAE 18:2 (AUC: 0.948), and NAE 20:0 (AUC: 0.895) were identified as potential diagnostic biomarkers for ICPP. Following GnRHa treatment, serum peak LH and FSH levels decreased, alongside reversible changes in 44 lipids, predominantly CAR, NAE, and TG. In conclusion, this study demonstrates that ICPP is associated with significant alterations in lipid metabolism. The lipidomic changes induced by GnRHa therapy suggest a potential link between ICPP treatment and lipid homeostasis, which warrants further investigation to refine therapeutic approaches for ICPP and potentially mitigate associated metabolic disorders.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143554720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0