Biomedical Chromatography最新文献_第9页

Intestinal Metabolic Pathways and Key Metabolites of Phytoestrogenic Fractions From Cuscutae Semen in Estrogen-Deficient Rats: Insights From UPLC-Q-TOF MS 雌激素缺乏大鼠菟丝子精液中植物雌激素组分的肠道代谢途径和关键代谢物：UPLC-Q-TOF质谱分析

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-07-08 DOI: 10.1002/bmc.70543

Mingyao Zhao, Bing Tian, Lizhu Zhao, Yingxiang Chen, Xiangming Sun, Hui Song

{"title":"Intestinal Metabolic Pathways and Key Metabolites of Phytoestrogenic Fractions From Cuscutae Semen in Estrogen-Deficient Rats: Insights From UPLC-Q-TOF MS","authors":"Mingyao Zhao, Bing Tian, Lizhu Zhao, Yingxiang Chen, Xiangming Sun, Hui Song","doi":"10.1002/bmc.70543","DOIUrl":"10.1002/bmc.70543","url":null,"abstract":"<div>\u0000 \u0000 <p>While flavonoids from <i>Cuscutae Semen</i> (CS) exert significant estrogen-regulating effects, their intestinal metabolism under estrogen-deficient conditions remains poorly understood. This study aimed to elucidate the metabolic fate of these active constituents. Using ovariectomized rats, we collected ileal sac fluid, colonic sac fluid, hepatic portal blood, and feces to establish metabolic profiles along the “ileum-colon-hepatic portal vein-feces” axis via UPLC-Q-TOF-MS. By employing a combined approach to evaluate both mucosa-mediated and microbiota-mediated metabolism, we identified 37 parent compounds (predominantly flavonoids) and 48 metabolites. The ileum and colon were confirmed as primary metabolic sites, predominantly involving Phase I and Phase II reactions. Metabolic pathways were detailed using quercetin as a representative phytoestrogen. Furthermore, we analyzed the intestinal metabolic patterns of nine in vivo direct-acting compounds highly correlated with CS's estrogenic effects. Collectively, this study uncovers the intestinal metabolic characteristics of CS active fractions under estrogen deficiency, providing a crucial foundation for future in vivo studies and elucidating the pharmacologically active basis of CS.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 8","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148410172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development, Validation, and Application of an LC–MS/MS Method for Pharmacokinetic Studies for the Datopotamab Deruxtecan-Dlnk Quantification in Rat Plasma 大鼠血浆中Datopotamab deruxtecan - dlink定量药代动力学研究的LC-MS/MS方法的开发、验证和应用。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-07-08 DOI: 10.1002/bmc.70542

Lurdhu Mary K, Naresh Podila, Venkata Ramana Maddula

{"title":"Development, Validation, and Application of an LC–MS/MS Method for Pharmacokinetic Studies for the Datopotamab Deruxtecan-Dlnk Quantification in Rat Plasma","authors":"Lurdhu Mary K, Naresh Podila, Venkata Ramana Maddula","doi":"10.1002/bmc.70542","DOIUrl":"10.1002/bmc.70542","url":null,"abstract":"<div>\u0000 \u0000 <p>To quantify datopotamab deruxtecan-dlnk in rat plasma, a selective, sensitive, and quick LC–MS/MS technique was created, validated, and successfully used in pharmacokinetic investigations. The analyte separation was accomplished on an Agilent Eclipse XDB-C18 analytical column (150 × 4.6 mm, 3.5 μm particle size) employing isocratic elution using an acetonitrile and buffer mobile phase (50:50, v/v) delivered at 1.0 mL/min. The internal standard was tisotumab vedotin. Mass spectrometric analysis was conducted using electrospray ionization (ESI) operated in the multiple reaction monitoring (MRM) mode. A simple and efficient protein precipitation technique was employed to prepare the sample. The method demonstrated remarkable linearity within the range of 25–1000 ng/mL, with an <i>r</i><sup>2</sup> value exceeding 0.999. Accuracy values ranged between 93.76% and 106.23%, with corresponding intraday and interday precision values being within reasonable bounds. There were no discernible matrix effects, and recovery was reliable and repeatable. Datopotamab deruxtecan-dlnk was found to be stable under all tested conditions, according to stability studies. After intravenous injection, the validated approach was effectively applied to pharmacokinetic research in rats, allowing for accurate estimates of important pharmacokinetic parameters. This approach is ideal for routine bioanalysis and pharmacokinetic evaluation of datopotamab deruxtecan-dlnk in preclinical research.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 8","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148403434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging Trends and Advancements in HPLC Technique: The Role of Nanotechnology, Machine Learning, and AI Optimization HPLC技术的新趋势和进展：纳米技术、机器学习和人工智能优化的作用。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-07-07 DOI: 10.1002/bmc.70535

Samar H. Elagamy

{"title":"Emerging Trends and Advancements in HPLC Technique: The Role of Nanotechnology, Machine Learning, and AI Optimization","authors":"Samar H. Elagamy","doi":"10.1002/bmc.70535","DOIUrl":"10.1002/bmc.70535","url":null,"abstract":"<div>\u0000 \u0000 <p>High-performance liquid chromatography (HPLC) plays a central role in pharmaceutical and biomedical analysis owing to its exceptional separation efficiency, sensitivity, and versatility. It plays a crucial role in the separation, identification, and quantification of a broad range of compounds, including pharmaceuticals, endogenous biomolecules, metabolites, and biomarkers in complex biological matrices such as blood, plasma, urine, and tissue samples. Continuous advances in HPLC technique such as column technology, system configuration, and data processing have significantly expanded its separation capability, sensitivity, and efficiency. This review provides an overview of recent and emerging trends in HPLC with emphasis on multidimensional and multimodal strategies, advanced stationary-phase materials such as covalent organic frameworks, core–shell particles, and nanomaterial-modified phases. Additionally, the application of deep eutectic solvents as green and tunable mobile-phase components, and the integration of supercritical fluid chromatography as a complementary high-efficiency separation approach are discussed. Furthermore, the growing application of artificial intelligence AI and machine learning ML tools in HPLC method development and optimization is critically reviewed. This review also discusses future perspectives in HPLC, with emphasis on advances in stationary phases, miniaturization, and improved sustainability.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 8","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148395632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism of Compound Xinyi Nasal Drops in the Treatment of Allergic Rhinitis Through STAT3/IL-17A/NF-κB Signaling Pathway Based on Network Pharmacology and Experimental Verification 复方心益滴鼻液通过STAT3/IL-17A/NF-κB信号通路治疗变应性鼻炎的网络药理学机制及实验验证

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-07-06 DOI: 10.1002/bmc.70540

Congwen Gu, Jiaxin Tan, Shuang Gen, Gang Cao, Yan Hong, Yanquan Han

{"title":"Mechanism of Compound Xinyi Nasal Drops in the Treatment of Allergic Rhinitis Through STAT3/IL-17A/NF-κB Signaling Pathway Based on Network Pharmacology and Experimental Verification","authors":"Congwen Gu, Jiaxin Tan, Shuang Gen, Gang Cao, Yan Hong, Yanquan Han","doi":"10.1002/bmc.70540","DOIUrl":"10.1002/bmc.70540","url":null,"abstract":"<div>\u0000 \u0000 <p>Compound Xinyi nasal drops (CXND) is a traditional Chinese medicine nasal drop developed from an empirical clinical prescription for the treatment of allergic rhinitis and has been used for more than 20 years, with precise therapeutic efficacy. However, the mechanism of action of CXND in treating allergic rhinitis remains unclear. Investigating the impact of CXND on allergic rhinitis and further defining its potential active ingredients and mechanism of action were the objectives of this study. The effective components of CXND were determined by ultra-performance liquid chromatography, and the core genes and important pathways of CXND in the treatment of AR were analyzed by network pharmacology. Then, molecular docking verified component-target binding activity. The guinea pig model was induced by OVA to study the effects of CXND on the STAT3/IL-17A/NF-κB signaling pathway and related inflammatory factors. The fingerprint spectrum of CXND was established, and seven effective components were identified. It was found that the seven components might exert their therapeutic effects on allergic rhinitis through the STAT3/IL-17A/NF-κB signaling pathway. In vivo experiments have found that CXND can effectively reduce the levels of IL-6, IL-17A, and TNF-α while inhibiting the expression levels of STAT3, IL-17A, NF-κB p65 mRNA, and their phosphorylated proteins.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 8","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148395674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Mechanism of Baicalin in Ameliorating Chronic Pancreatitis: Insights From Network Pharmacology and Metabolomics 黄芩苷改善慢性胰腺炎的分子机制：来自网络药理学和代谢组学的见解。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-07-03 DOI: 10.1002/bmc.70534

Ruyang Cheng, Weining Song, Yu Wang, Yehao Wang, Xiaomin Xu, Fang Lu, Donghua Yu, Pingping Chen, Shumin Liu

{"title":"Molecular Mechanism of Baicalin in Ameliorating Chronic Pancreatitis: Insights From Network Pharmacology and Metabolomics","authors":"Ruyang Cheng, Weining Song, Yu Wang, Yehao Wang, Xiaomin Xu, Fang Lu, Donghua Yu, Pingping Chen, Shumin Liu","doi":"10.1002/bmc.70534","DOIUrl":"10.1002/bmc.70534","url":null,"abstract":"<div>\u0000 \u0000 <p>In this study, we explored the therapeutic effects and mechanisms of baicalin (BA) in a dibutyltin dichloride (DBTC)–induced chronic pancreatitis (CP) rat model using metabolomics and network pharmacology. The results showed that BA significantly reduced inflammatory cell infiltration and fibrosis in pancreatic tissues, lowered serum levels of interleukin (IL)-1β, IL-6, tumor necrosis factor-alpha (TNF-α), amylase (AMS), and lipopolysaccharide (LPS) and thereby decreased expression of alpha-smooth muscle actin (α-SMA) and fibronectin (FN) in pancreatic tissues. Metabolomic analyses revealed that BA influences multiple metabolic pathways, including glycerophospholipid and arachidonic acid metabolism, in serum and urine. Network pharmacology identified key targets, such as phospholipase A2 group IVA (PLA2G4A) and transient receptor potential vanilloid 4 (TRPV4), which are primarily involved in IL-17 signaling, as well as other pathways beyond inflammation. Further integrated analysis demonstrated that BA inhibits pancreatic stellate cell activation and reduces pancreatic fibrosis by regulating metabolites, such as arachidonic acid, that act on the PLA2G4A/TRPV4 and transforming growth factor-beta 1 (TGF-β1)/SMAD family member 3 (SMAD3) pathways. Molecular docking studies confirmed that BA binds strongly to these targets. In summary, BA exerts anti-inflammatory and antifibrotic effects through multiple targets and pathways, thereby alleviating the pathological progression of CP.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 8","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148374571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Determination of Levetiracetam, Sodium Valproate, and Olanzapine in Human Plasma by UPLC-MS/MS for Therapeutic Drug Monitoring UPLC-MS/MS同时测定人血浆中左乙拉西坦、丙戊酸钠和奥氮平治疗药物监测。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-07-02 DOI: 10.1002/bmc.70536

Feng Zhou, Xinjun Cai, Chongyang Wu

引用次数: 0

LC–MS/MS-Based Monitoring of Pregabalin and Gabapentin Reveals Marked Pharmacokinetic Variability and Limited Exposure–Response Relationships in Neuropathic Pain Patients 基于LC-MS/ ms的普瑞巴林和加巴喷丁监测揭示了神经性疼痛患者明显的药代动力学变异性和有限的暴露-反应关系。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-07-02 DOI: 10.1002/bmc.70539

Duygu Eryavuz Onmaz, Fettah Eren, Kadir Gulsen, Firdevs Sak, Mohammad Ahmad Bik, Rabia Coban, Sedat Abusoglu, Ali Unlu

{"title":"LC–MS/MS-Based Monitoring of Pregabalin and Gabapentin Reveals Marked Pharmacokinetic Variability and Limited Exposure–Response Relationships in Neuropathic Pain Patients","authors":"Duygu Eryavuz Onmaz, Fettah Eren, Kadir Gulsen, Firdevs Sak, Mohammad Ahmad Bik, Rabia Coban, Sedat Abusoglu, Ali Unlu","doi":"10.1002/bmc.70539","DOIUrl":"10.1002/bmc.70539","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>This study aimed to develop and validate a liquid chromatography–tandem mass spectrometry (LC–MS/MS) assay for the simultaneous quantification of pregabalin (PGB) and gabapentin (GBP) and to explore real-world pharmacokinetic variability. A bioanalytical LC–MS/MS method was validated using serum samples prepared by acetonitrile protein precipitation. Chromatographic separation was achieved on a C18 column with gradient elution and positive electrospray ionization. The validated method was applied to serum samples obtained from 65 patients, and associations between serum drug concentrations and clinical measures were evaluated. The assay demonstrated excellent linearity (<i>R</i>² > 0.998), acceptable precision and accuracy, recovery ranging from 96.9% to 101.5%, and matrix effects below 7%. Considerable inter-individual variability in serum concentrations was observed (PGB: median 700 ng/mL [IQR: 500–1000]; GBP: median 4500 ng/mL [IQR: 3200–5700]). No significant dose–concentration relationships were identified (PGB: <i>ρ</i> = 0.03; GBP: <i>ρ</i> = −0.08), and no significant associations were observed between serum concentrations and the clinical outcome measures assessed. In contrast, both drugs showed moderate inverse correlations with post-dose sampling interval (PGB: <i>ρ</i> = −0.46; GBP: <i>ρ</i> = −0.45). These findings indicate substantial pharmacokinetic variability of gabapentinoids under routine clinical conditions. Although no clear dose–concentration or exposure–response relationships were identified in this cohort, interpretation should be made cautiously because of non-standardized sampling conditions and the relatively narrow range of renal function among participants.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 8","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148374607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sophora tonkinensis Alleviates Progression of Diethylnitrosamine-Induced Hepatocellular Carcinoma in Rats via Multitarget Regulation. 苦参通过多靶点调控减轻二乙基亚硝胺诱导的大鼠肝细胞癌的进展。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-07-01 DOI: 10.1002/bmc.70486

Zongliang Lu, Jincai Wen, Chengwei Li, Yuanyuan Guo, Siqi Huang, Xianling Wang, Xiaoyan Chen, Yingjie Xu, Xiaoyan Zhan, Xiaohe Xiao, Lele Zhang, Zhaofang Bai

{"title":"Sophora tonkinensis Alleviates Progression of Diethylnitrosamine-Induced Hepatocellular Carcinoma in Rats via Multitarget Regulation.","authors":"Zongliang Lu, Jincai Wen, Chengwei Li, Yuanyuan Guo, Siqi Huang, Xianling Wang, Xiaoyan Chen, Yingjie Xu, Xiaoyan Zhan, Xiaohe Xiao, Lele Zhang, Zhaofang Bai","doi":"10.1002/bmc.70486","DOIUrl":"10.1002/bmc.70486","url":null,"abstract":"<p><p>Sophora tonkinensis, a traditional Chinese medicine with heat-clearing, detoxifying, and throat-soothing properties, has shown potential in treating hepatitis, inhibiting tumors, and regulating immunity, but its active constituents and mechanisms remain unclear. We established a hepatocellular carcinoma (HCC) model in SD rats using diethylnitrosamine (DEN), with oral gavage of S. tonkinensis extract (STE) from Weeks 1 to 16, alongside model and normal control groups. Results showed that STE significantly reduced HCC incidence, reversed weight loss, decreased the number and volume of liver nodules, improved histopathological damage, and lowered serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and albumin (ALB) levels. Integrated transcriptomic (RNA-seq) and metabolomic (LC-MS/MS) analyses revealed enrichment in arachidonic acid and glycerophospholipid metabolism. Quantitative real-time polymerase chain reaction (qPCR) validation confirmed that STE restored hepatic lipid homeostasis and alleviated inflammation by activating the peroxisome proliferator-activated receptor (PPAR) pathway and suppressing the IL-17/TGF-β axis. These findings clarify the core mechanisms of STE in preventing and treating HCC, providing a theoretical basis for its precise clinical application.</p>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 7","pages":"e70486"},"PeriodicalIF":1.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147947711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Evaluation of Growth Period Variations in Bioactive Properties and Phytochemical Profiles of Aesculus chinensis Bge Seeds and Leaves. 七叶神马种子和叶片生物活性特性及植物化学特征的生育期变化综合评价

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-07-01 DOI: 10.1002/bmc.70490

Yu Liu, Dongju Ren, Yanchen Liu, Na Li, Xiaoxue Jiang, Yanyan Zhang, Rongxia Liu

{"title":"Comprehensive Evaluation of Growth Period Variations in Bioactive Properties and Phytochemical Profiles of Aesculus chinensis Bge Seeds and Leaves.","authors":"Yu Liu, Dongju Ren, Yanchen Liu, Na Li, Xiaoxue Jiang, Yanyan Zhang, Rongxia Liu","doi":"10.1002/bmc.70490","DOIUrl":"10.1002/bmc.70490","url":null,"abstract":"<p><p>In China, Aesculus chinensis Bge (A.Bge) is known as a medicinal plant. A.Bge seeds have been traditionally used to relieve abdominal distention and stomach pain, whereas studies on A.Bge leaves remain limited. This study aimed to clarify the dynamic bioactivities and chemical profiles of A.Bge seeds and leaves across their growth phases and to identify potential biomarkers specific to each organ. A.Bge seeds and leaves collected in different months were evaluated for anti-inflammatory, hypolipidemic, and hypoglycemic activities. Phytochemical constituents were characterized using UHPLC-HRMS combined with chemometrics analysis, followed by quantitative determination of potential biomarkers. Leaves collected in October showed comparable anti-inflammatory and hypolipidemic effects to seeds collected in October, with a slight reduction in α-glucosidase inhibitory efficacy. Among 123 identified components in seeds and leaves, terpenoids, flavonoids, and phenylpropanoids were predominant. Despite exhibiting comparable bioactivities, seeds and leaves were associated with different bioactive components. Specifically, nine bioactivity-related contributors in seeds, including escin IA and alpha-linolenic acid, and 12 in leaves, such as kaempferol and hyperoside, were successfully determined. The integration of phytometabolomics and pharmacodynamics provides an effective strategy to identify potential bioactive contributors in A.Bge seeds and leaves, thereby facilitating their future pharmacological application and quality control.</p>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 7","pages":"e70490"},"PeriodicalIF":1.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147961965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UPLC-MS/MS-Based Cellular Pharmacokinetics of Four Active Components of Total Glucosides of Picrorhizae Rhizome and Target Validation for ACLY. 基于UPLC-MS/ ms的苦根茎总苷四种有效成分的细胞药动学及ACLY靶标验证

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-07-01 DOI: 10.1002/bmc.70489

Yifang Zhang, Xiangyang Wang, Miao Qiao, Yu Liu, Tianlei Liu, Shuang Fu, Yang Chu, Genbei Wang, Xiaohui Ma

{"title":"UPLC-MS/MS-Based Cellular Pharmacokinetics of Four Active Components of Total Glucosides of Picrorhizae Rhizome and Target Validation for ACLY.","authors":"Yifang Zhang, Xiangyang Wang, Miao Qiao, Yu Liu, Tianlei Liu, Shuang Fu, Yang Chu, Genbei Wang, Xiaohui Ma","doi":"10.1002/bmc.70489","DOIUrl":"10.1002/bmc.70489","url":null,"abstract":"<p><p>Total glucosides of picrorhizae rhizome (TGPR) are a promising candidate drug for treating non-alcoholic steatohepatitis (NASH). To clarify the material basis underlying its efficacy, it is crucial to characterize the cellular pharmacokinetics of TGPR. A highly sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established in this study for the simultaneous quantification of picroside I, II, III, and IV from TGPR in HepG2 cells and their subcellular fractions (nucleus, mitochondria, cytoplasm). Additionally, molecular docking and cellular thermal shift assay (CETSA) were employed to investigate the binding of TGPR to ATP-citrate lyase (ACLY). The results showed that the analytical method was fully validated and within acceptable limits. All four TGPR components could enter HepG2 cells, reaching peak time at approximately 1.5 h, with picroside II exhibiting the highest exposure levels. In the nucleus and cytoplasm, picroside I and II exhibited relatively high affinity and did not reach saturation within 24 h. CETSA and molecular docking confirmed that TGPR directly bound to ACLY to enhance its thermal stability. In conclusion, this study first reveals the cellular pharmacokinetic characteristics of TGPR in HepG2 cells and identifies ACLY as its direct target, providing scientific evidence for further exploring the potential targets and mechanisms of TGPR.</p>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 7","pages":"e70489"},"PeriodicalIF":1.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147925901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0