Biomedical Chromatography最新文献_第9页

A UHPLC–MS/MS Method Reveals the Pharmacokinetics of Deacetyl Asperulosidic Acid Methyl Ester in Rats UHPLC-MS /MS法研究脱乙酰曲霉酸甲酯在大鼠体内的药动学

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-07 DOI: 10.1002/bmc.70001

Huida Guan, Jian Chen, Hao Yin, Xia Feng, Chang Liu, Shanshan Liu, Jiacheng Li, Jingchu Li, Yongbing Cao, Chao Ma

{"title":"A UHPLC–MS/MS Method Reveals the Pharmacokinetics of Deacetyl Asperulosidic Acid Methyl Ester in Rats","authors":"Huida Guan, Jian Chen, Hao Yin, Xia Feng, Chang Liu, Shanshan Liu, Jiacheng Li, Jingchu Li, Yongbing Cao, Chao Ma","doi":"10.1002/bmc.70001","DOIUrl":"https://doi.org/10.1002/bmc.70001","url":null,"abstract":"<div>\u0000 \u0000 <p>In the current study, a simple ultra-high performance liquid chromatography–tandem mass spectrometry method was developed and fully validated for the quantitation of deacetyl asperulosidic acid methyl ester in rat plasma. The plasma sample was precipitated with acetonitrile and then separated on the Waters ACQUITY UPLC HSS T3 column. The mobile phases, water and acetonitrile, were added with 0.1% formic acid. The mass spectrometry detection was performed in negative-ion multiple reaction monitoring. In the range of 1–1000 ng/mL, the linearity meets the requirements with correlation coefficient more than 0.99. The parameters of accuracy, precision, carryover, matrix effect, extraction recovery, stability, and dilution integrity are within accepted ranges. The validated method has been successfully used for pharmacokinetic study of deacetyl asperulosidic acid methyl ester in rats. After oral administration, deacetyl asperulosidic acid methyl ester was quickly absorbed into blood and reached the maximum plasma drug concentration of 4047.49 ng/mL at 2 h. The half-life of deacetyl asperulosidic acid methyl ester is 5.6 h, which suggests that it has a moderate metabolic process. Since the absolute bioavailability of deacetyl asperulosidic acid methyl ester is only 3.74%, its gastrointestinal stability, first-pass effect, and transmembrane properties remain to be studied.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combining 16S rRNA Gene Sequencing and Metabolomics to Study the Therapeutic Effects of Sangge Jiangzhi Pill on Hyperlipidemia Rats 结合16S rRNA基因测序和代谢组学研究三格降脂丸对高脂血症大鼠的治疗作用

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-07 DOI: 10.1002/bmc.6089

Xuan Pan, Jianhai Wang, Shuo Liu, Ruonan Yu, Ziyu Li, Yumeng Zhang, Miao Wang, Chunjie Zhao, Min Zhao

{"title":"Combining 16S rRNA Gene Sequencing and Metabolomics to Study the Therapeutic Effects of Sangge Jiangzhi Pill on Hyperlipidemia Rats","authors":"Xuan Pan, Jianhai Wang, Shuo Liu, Ruonan Yu, Ziyu Li, Yumeng Zhang, Miao Wang, Chunjie Zhao, Min Zhao","doi":"10.1002/bmc.6089","DOIUrl":"https://doi.org/10.1002/bmc.6089","url":null,"abstract":"<div>\u0000 \u0000 <p>Sangge Jiangzhi pill, as a traditional Chinese medicine compound preparation, can be used to treat hyperlipidemia. However, the specific material basis and mechanism of action of the Sangge Jiangzhi pill are not clear. In this study, gut microbiota and host metabolism were investigated using 16S rRNA sequencing and metabolomics techniques. The changes in body weight, organ index, serum biochemical levels, and liver pathology demonstrated that the Sangge Jiangzhi pill reduced body weight and attenuated fat accumulation in hyperlipidemic rats. The results of gut microbiota analysis demonstrated that the Sangge Jiangzhi pill significantly enhanced the richness and diversity of gut microbiota in hyperlipidemia rats. Moreover, it regulated the relative abundance of <i>Ligilactobacillus</i>, <i>Limosilactobacillus</i>, <i>Lactobacillus</i>, <i>Blautia</i>, <i>Parabacteroides</i>, <i>Helicobacter</i>, <i>Colidextribacter</i>, <i>Desulfovibrio</i>, <i>Rikenella</i>, and <i>Tuzzerella</i> to protect the intestinal barrier and relieve the oxidative stress reaction. Metabonomics analysis showed that a total of 32 differential metabolites were identified in feces. The results of the correlation analysis revealed a significant association between intestinal microbiota and fecal metabolites. These results suggested that the Sangge Jiangzhi pill could achieve the purpose of treating hyperlipidemia by regulating gut microbiota imbalance and metabolic activity disorder.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Chromatographic Analysis of Amino Acids in the Seeds of Three Nigella L. Representatives 三种黑属植物种子氨基酸的比较色谱分析

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-07 DOI: 10.1002/bmc.70009

Mariia Shanaida, Olha Korablova, Dzhamal Rakhmetov, Oksana Serafyn, Neonila Lashch, Volodymyr Shanaida

{"title":"Comparative Chromatographic Analysis of Amino Acids in the Seeds of Three Nigella L. Representatives","authors":"Mariia Shanaida, Olha Korablova, Dzhamal Rakhmetov, Oksana Serafyn, Neonila Lashch, Volodymyr Shanaida","doi":"10.1002/bmc.70009","DOIUrl":"https://doi.org/10.1002/bmc.70009","url":null,"abstract":"<div>\u0000 \u0000 <p>Amino acids are an important class of primary metabolites that can exhibit various pharmacological activities. In this study, the comparative gas chromatography–mass spectrometry (GC/MS) analysis of free and total amino acids in the seeds of three representatives of the <i>Nigella</i> L. genus (<i>Nigella arvensis</i> L., <i>Nigella damascena</i> L. [cultivar “Rizdvyana zirochka”], and <i>Nigella sativa</i> L. [cultivar “Faraon”]) was conducted under their cultivation in Ukraine. Regarding the free state of amino acids, 11 were detected in the raw material of <i>N. damascena</i>, and 9 were found in both <i>N. sativa</i> and <i>N. arvensis</i>. It was found the highest content of L-aspartic acid, specifically 50.359 mg/g in <i>N. damascena</i> (cultivar “Rizdvyana zirochka”), 33.816 mg/g in <i>N. sativa</i> (cultivar “Faraon”), and 31.028 mg/g in <i>N. arvensis</i>. It was revealed that L-leucine, L-valine, and L-tyrosine dominated among the essential amino acids detected in the free state in the seeds of all studied samples. L-glycine and L-proline had the highest amounts among the semiessential amino acids, while L-aspartic acid, L-glutamic acid, and L-phenylalanine are predominant in the nonessential group. Some of the detected in the <i>Nigella</i> sp. amino acids possess significant therapeutic potential.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and Identification of the Metabolites of Tuxobertinib in Rat, Dog, Monkey, and Human Liver Microsomes by Liquid Chromatography Combined With High Resolution Mass Spectrometry 用液相色谱-高分辨质谱联用方法鉴定大鼠、狗、猴和人肝微粒体中Tuxobertinib代谢物

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-07 DOI: 10.1002/bmc.70003

Pei Liu, Jing Huang, Yao Zhao, Jing Zhang, Qianrong Xue, Shuqin Zhang

{"title":"Characterization and Identification of the Metabolites of Tuxobertinib in Rat, Dog, Monkey, and Human Liver Microsomes by Liquid Chromatography Combined With High Resolution Mass Spectrometry","authors":"Pei Liu, Jing Huang, Yao Zhao, Jing Zhang, Qianrong Xue, Shuqin Zhang","doi":"10.1002/bmc.70003","DOIUrl":"https://doi.org/10.1002/bmc.70003","url":null,"abstract":"<div>\u0000 \u0000 <p>Tuxobertinib is an EGFR tyrosine kinase inhibitor, which was undergoing clinical development for the treatment of non-small cell lung cancer (NSCLC). The aim of this study was to characterize the metabolites of tuxobertinib in liver microsomes and recombinant cytochrome P450 enzymes and to propose the metabolic pathways. The metabolites were generated by individually incubating tuxobertinib (5 μM) with rat, dog, monkey, and human liver microsomes, NADPH, and at 37°C for 60 min. The samples were analyzed by ultra-high-performance liquid chromatography-quadrupole/orbitrap mass spectrometry (UPLC-Q/Orbitrap-MS) using electrospray ionization in positive ion mode. The metabolites were characterized and identified by their accurate MS and MS/MS data. Totally, 18 metabolites were detected, and their structure was characterized. All these metabolites were -NADPH-dependent. The metabolic pathways of tuxobertinib included O-dealkylation, hydroxylation, oxidative deamination, dehydrogenation, and the opening of morpholine. M4 (O-dealkylation) was the major metabolite in all species. No human specific metabolite was observed. In human recombinant CYP3A4, 13 metabolites were detected, and CYP3A4 was the primary enzyme responsible for tuxobertinib metabolism. This is the first report on the metabolism of tuxobertinib, which provided an overview of the metabolism profiles of tuxobertinib in vitro, which is helpful in understanding its safety and action.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Derivatization-Based Densitometric Approach Along With Greenness Assessment for Simultaneous Evaluation of Duloxetine Hydrochloride and Pregabalin: An Application to Pharmaceutical Analysis 基于衍生化的密度测定方法和绿度评估用于同时评估盐酸度洛西汀和普瑞巴林：在药物分析中的应用

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-07 DOI: 10.1002/bmc.6078

Susheel John Varghese, Karthikeyan Venkatesan, Thengungal Kochupappy Ravi

{"title":"Derivatization-Based Densitometric Approach Along With Greenness Assessment for Simultaneous Evaluation of Duloxetine Hydrochloride and Pregabalin: An Application to Pharmaceutical Analysis","authors":"Susheel John Varghese, Karthikeyan Venkatesan, Thengungal Kochupappy Ravi","doi":"10.1002/bmc.6078","DOIUrl":"https://doi.org/10.1002/bmc.6078","url":null,"abstract":"<div>\u0000 \u0000 <p>The fixed-dose combination of pregabalin and duloxetine hydrochloride is mainly used in the treatment of neuropathic pain. Sensitive determination of drugs like pregabalin is challenging by chromatographic methods using UV detection due to their nonchromophoric nature. This study presents the development of a validated high-performance thin-layer chromatography (HPTLC) method for the simultaneous determination pregabalin and duloxetine hydrochloride in formulation. Because pregabalin has a weak chromophoric system, it was visualized using a derivatization reagent containing ninhydrin. Optimum separation of pregabalin and duloxetine hydrochloride was achieved on silica gel 60 F<sub>254</sub> plates using methanol:dichloromethane:acetone:ammonia (8:1:1:0.2, v/v/v/v). Good resolution was achieved with R<sub>f</sub> values of 0.34 ± 0.02 and 0.52 ± 0.02 for duloxetine hydrochloride and pregabalin, respectively. The method was validated as per the ICH Q2(R1) guidelines. Linearity range was found between 200 and 450 ng/band for duloxetine and 500 and 1125 ng/band for pregabalin. Low %RSD values shows that the developed method is precise. Greenness assessment of the developed method was evaluated using three different assessment tools, namely, Analytical Eco-Scale, GAPI, and AGREE This is the first validated derivatization-based HPTLC method for simultaneous analysis of duloxetine hydrochloride and pregabalin.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taguchi L9 Optimization of Microwave-Assisted Solid–Liquid Duo Heating for Berberine Extraction With HPTLC Analysis 微波辅助固液双加热提取小檗碱的高效液相色谱优化

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-07 DOI: 10.1002/bmc.70008

Saurabh Satija, Manish Vyas, Parijat Pandey, Munish Garg

{"title":"Taguchi L9 Optimization of Microwave-Assisted Solid–Liquid Duo Heating for Berberine Extraction With HPTLC Analysis","authors":"Saurabh Satija, Manish Vyas, Parijat Pandey, Munish Garg","doi":"10.1002/bmc.70008","DOIUrl":"https://doi.org/10.1002/bmc.70008","url":null,"abstract":"<p>We present an optimized approach for the extraction of berberine using microwave-assisted solid–liquid duo heating. The conventional procedure relies on the simultaneous heating of the sample matrix and the dissolving solvent under microwave energy. To improve the efficiency of this process, we employed the Taguchi L9 orthogonal design methodology, focusing on key extraction parameters, including microwave power, irradiation time, solute-solvent ratio, and temperature. Berberine content was quantified using high-performance thin-layer chromatography (HPTLC). Our findings revealed that the most influential parameters in the berberine extraction process were microwave power (ranked as the most critical factor), followed by irradiation time (second in importance), and temperature (third in significance). Utilizing the dual heating approach in microwave-assisted extraction (MAE) led to a more precise and efficient determination of extraction parameters, resulting in enhanced accuracy and higher yield. Additionally, this optimized method significantly reduced the overall extraction time, making it an ideal choice for berberine extraction under optimal conditions.</p>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bmc.70008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of Main Aminothiols in Blood Plasma Using Capillary Electrophoresis With UV Detection 毛细管电泳-紫外检测法测定血浆中主要氨基硫醇

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-07 DOI: 10.1002/bmc.70017

Polina Alexandrovna Pudova, Alexander Vladimirovich Ivanov, Mikhail Aleksandrovich Popov, Ruslan Andreevich Maslennikov, Valery Vasil'evich Aleksandrin, Maria Pavlovna Galdobina, Aslan Amirkhanovich Kubatiev

{"title":"Determination of Main Aminothiols in Blood Plasma Using Capillary Electrophoresis With UV Detection","authors":"Polina Alexandrovna Pudova, Alexander Vladimirovich Ivanov, Mikhail Aleksandrovich Popov, Ruslan Andreevich Maslennikov, Valery Vasil'evich Aleksandrin, Maria Pavlovna Galdobina, Aslan Amirkhanovich Kubatiev","doi":"10.1002/bmc.70017","DOIUrl":"https://doi.org/10.1002/bmc.70017","url":null,"abstract":"<div>\u0000 \u0000 <p>A new approach of total major aminothiols determination (cysteine, homocystine, cysteinylglycine, and glutathione) in human plasma by capillary electrophoresis (CE) is presented. It is based on the analytes reduction by tris (2-carboxyethyl) phosphine and derivatization with N-ethylmaleimide, with subsequent ultrafiltration of the sample. Separation was performed using 0.1-M phosphate Na рН 2.48 with 1% Ficoll-400 and 10% methanol. The in-capillary concentration of the analytes was achieved with a pH gradient created via the preinjection of triethanolamine. The absorbance signal was detected at 200 nm. The total analysis time was 16 min (using a silica capillary [50 μm i.d.; total length 42 cm] at a 20-kV voltage). The limits of detection varied from 1.3 μM (glutathione) to 5.4 μM (cysteine). Within-run and between-run precision (CV) was < 7%; reduction of analytes ranging from 91% to 106%, were obtained. The approach was validated on control samples of children's blood plasma. The simplicity of the method and the availability of reagents make the presented approach most suitable for use on standard <span>CE</span> systems.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Validated Headspace Gas Chromatographic Method for the Determination of Ethyl Alcohol in Food Supplements 顶空气相色谱法测定食品添加剂中乙醇含量

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-07 DOI: 10.1002/bmc.70002

Sedat Sel, Serap Saglik Aslan

{"title":"A Validated Headspace Gas Chromatographic Method for the Determination of Ethyl Alcohol in Food Supplements","authors":"Sedat Sel, Serap Saglik Aslan","doi":"10.1002/bmc.70002","DOIUrl":"https://doi.org/10.1002/bmc.70002","url":null,"abstract":"<div>\u0000 \u0000 <p>Food supplements are defined as products containing nutritional elements such as vitamins, minerals, amino acids, and herbal components that cannot be taken in adequate amounts with normal nutrition. Ethyl alcohol (EtOH) is used in the production and standardization of some food supplements. Even a little MeOH can cause serious health problems in EtOH. Authorized institutions issuing halal product certificates consider foods containing more than 1% alcohol to be haram. In this study, new, reliable, sensitive, and fast GC-FID and HS GC-FID methods were developed, validated, and compared to determine the amount of EtOH and MeOH that can be found in food supplements. Chromatographic separation was performed with DB-WAX (30 m × 0.32 mm × 0.5 μm) and TC-WAX (30 m × 0.32 mm × 0.5 μm) in the GC-FID and HS GCFID methods, respectively. Oven temperature program that increased 5°C/min from 80°C to 100°C in 40:1 split condition was used. The calibration range is 0.002–0.1 g/dL. LOD and LOQ values are found as 0.00036 and 0.00050 g/dL and 0.0011 and 0.0015 g/dL for EtOH and 0.00050 and 0.00060 g/dL and 0.0015 and 0.0019 g/dL for MeOH by GC-FID and HS GC-FID methods, respectively. The developed and validated methods were applied to different food supplements commercially sold in the market with the label containing alcohol but not containing alcohol percentage and one food supplement with the label alcohol-free. The developed and validated GC-FID and HS GC-FID methods can be used in the determination of EtOH and MeOH in food supplements.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultra-High-Performance Liquid Chromatography Combined With Mass Spectrometry Detection Analytical Method for the Determination of N-Nitrosoduloxetine in Duloxetine HCl 测定盐酸度洛西汀中 N-亚硝基度洛西汀的超高效液相色谱-质谱检测分析方法

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-07 DOI: 10.1002/bmc.6084

Anna Marlés-Torres, Sergio Bessa-Jambrina, Cristóbal Galán-Rodríguez

{"title":"Ultra-High-Performance Liquid Chromatography Combined With Mass Spectrometry Detection Analytical Method for the Determination of N-Nitrosoduloxetine in Duloxetine HCl","authors":"Anna Marlés-Torres, Sergio Bessa-Jambrina, Cristóbal Galán-Rodríguez","doi":"10.1002/bmc.6084","DOIUrl":"https://doi.org/10.1002/bmc.6084","url":null,"abstract":"<div>\u0000 \u0000 <p>In the present study, one simple, fast, feasible, and sensitive ultra-high-performance liquid chromatography combined with mass spectrometry detection method, using electrospray ionization, is proposed. This method was developed and validated for the determination of <i>N</i>-nitrosoduloxetine content in Duloxetine HCl active pharmaceutical ingredient in 11 min employing a simple preparation method for both reference and sample solutions. The proposed method was validated as per regulatory guidelines. Acquity HSS T3 (3.0 × 100 mm, 1.8 μm) column and formic acid 0.1% in water combined with acetonitrile were used for chromatographic separation. The limit of detection and the limit of quantification—reporting threshold—were found to be 0.7 and 70 ppb, respectively. The trueness and precision of the method have been demonstrated in the working range, giving values of recovery within the range of 100%–110%, and the regression coefficients (<i>R</i>) were found to be in the range of 0.9990–0.9991. This method could be used for controlling <i>N</i>-nitrosoduloxetine content in duloxetine HCl drug substance batches manufactured at Moehs group.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic Potential of Artemisia campestris Essential Oil: Antioxidant, Anti-Inflammatory, and Anticancer Insights From In Silico Analysis 野蒿精油的治疗潜力：从硅学分析中洞察抗氧化、抗炎和抗癌潜力

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-07 DOI: 10.1002/bmc.70012

Inasse Cherfi, Mahboub Nasma, Gamil Gamal Hasan, Abir Benaissa, Youcef Benaissa, Salah Eddine Laouini, Abderrhmane Bouafia, Fahad Alharthi, Talha Bin Emran, Jewel Mallick

{"title":"Therapeutic Potential of Artemisia campestris Essential Oil: Antioxidant, Anti-Inflammatory, and Anticancer Insights From In Silico Analysis","authors":"Inasse Cherfi, Mahboub Nasma, Gamil Gamal Hasan, Abir Benaissa, Youcef Benaissa, Salah Eddine Laouini, Abderrhmane Bouafia, Fahad Alharthi, Talha Bin Emran, Jewel Mallick","doi":"10.1002/bmc.70012","DOIUrl":"https://doi.org/10.1002/bmc.70012","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Artemisia campestris</i> subsp. <i>campestris</i> (tuguft) is a medicinal plant traditionally used in Algerian medicine. This study investigates the chemical composition and bioactivity of its essential oil (ACEO). Gas chromatography–mass spectrometry (GC-MS) analysis identified key compounds, including linalyl acetate (2.92%), geranyl acetate (2.45%), and eucalyptol (1.38%). ACEO demonstrated significant antioxidant activity, with IC<sub>50</sub> values of 11.09 μg/mL (DPPH), 15.81 μg/mL (FRAP), and 22.70 μg/mL (<i>β-</i>carotene). It also enhanced peroxidase activity by 82.67 U/g. The anti-inflammatory effects were confirmed with an IC<sub>50</sub> of 18.87 μg/mL. Notably, in silico molecular docking revealed that 3-cyclopentyl-<i>N</i>-(2-(3,4-dimethoxyphenyl)ethyl) exhibits strong binding affinity to phosphoinositide 3-kinase gamma, a target for pancreatic cancer therapy, suggesting potential anticancer activity. These findings underscore the therapeutic potential of ACEO, highlighting its antioxidant, anti-inflammatory, and anticancer properties.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0