用UHPLC/-ESI-Q-TOF/MS研究奥丙烯酰亚胺E在大鼠血浆中的药动学

IF 1.8 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2025-04-20 DOI:10.1002/bmc.70067

Zheming Ying, Kaiyun Jiang, Chengyu Wang, Hongzhe Zhang, Junjie Yao, Yingdai Zhao, Xixiang Ying, Yanling Ren

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引用次数: 0

摘要

马齿苋新生物碱 Oleracrylimide E 具有显著的生物活性。随后，以牡荆素-2″-O-鼠李糖苷为内标物（IS），采用超高效液相色谱法评估了大鼠血浆中静脉注射 2.0 毫克/千克体重剂量的该生物碱的药代动力学。采用 UHPLC-ESI-Q-TOF/MS 方法研究了 Oleracrylimide E 在大鼠体内的代谢情况，以及口服后 Oleracrylimide E 立即转化为其他代谢物的情况。结果在实验鼠的血浆、尿液和粪便中总共鉴定出16种Oleracrylimide E的代谢物。

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Pharmacokinetics of Oleracrylimide E in Rats Plasma Using UHPLC/-ESI-Q-TOF/MS

Oleracrylimide E, a new alkaloid from Portulaca oleracea L., shows significant bioactivity. Then, the pharmacokinetics of this alkaloid after its intravenous administration at dose of 2.0 mg/kg body weight in rat plasma was evaluated using UHPLC with vitexin-2″-O-rhamnoside as an internal standard (IS). The UHPLC-ESI-Q-TOF/MS method was applied to investigate the metabolism of Oleracrylimide E in rats in response to the immediate conversion of Oleracrylimide E into other metabolites after its oral administration. As a result, a total of 16 metabolites of Oleracrylimide E were identified in the plasma, urine, and fecal of the experimental rats.

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.