Biomedical Chromatography最新文献

{"title":"Optimization of Environmentally Sustainable UPLC-PDA Method Using Box–Behnken for Simultaneous Quantifying Curcumin and Candesartan Cilexetil in On-Demand Formulation for Treatment of Brain Ischemic Injury","authors":"Abdelrahman Y. Sherif,&nbsp;Mohamed W. Attwa,&nbsp;Ehab M. Elzayat","doi":"10.1002/bmc.70130","DOIUrl":"https://doi.org/10.1002/bmc.70130","url":null,"abstract":"<div>\u0000 \u0000 <p>Brain ischemic injury is characterized by reduced cerebral blood flow and neuronal cell damage. Curcumin and candesartan cilexetil were proposed to restore normal brain function. Therefore, a validated UPLC method is required to quantify drugs within the proposed formulation. The Box–Behnken design was used to optimize the ultra-performance liquid chromatography (UPLC) method. The impact of three critical parameters (aqueous phase percentage, flow rate, and column temperature) on the measured responses was studied. Additionally, the method's environmental impact was assessed using AGREE software. Finally, drug content, dispersed formulation stability, and antioxidant activity were investigated. The optimal conditions utilized a mobile phase (0.1% formic acid:acetonitrile; 44:56% v/v) with a flow rate of 0.2 mL/min through an Acquity UPLC BEH C18 1.8 μm (2.1 × 50 mm) column maintained at 40°C. This resulted in efficient curcumin and candesartan cilexetil separation peaks with retention times of 1.49 and 6.35 min, respectively. The method demonstrated excellent linearity. The calculated intraday and interday precision values were &lt; 3.6% RSD with accuracy values (98.8%–105.1%). Moreover, the optimized UPLC method achieved a favorable eco-scale score of 0.77. The validated method successfully analyzed drug content within the SNEDDS formulation, which was able to solubilize both drugs and maintain the antioxidant activity of curcumin.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144244126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GC/MS Analysis of the Chemical Composition of Petroleum Ether and Chloroform Extracts From Coffea arabica Seeds, Along With Molecular Docking Evaluation of the Extracts Antibacterial and Anticancer Activities","authors":"Mohsen Mhamdi,&nbsp;Zarah I. Alzahrani,&nbsp;Hassen Harzali,&nbsp;Suliman A. Alderhami,&nbsp;Akram A. Hussain,&nbsp;Nashwa H. Abdullah,&nbsp;Ahmed A. Elhenawy,&nbsp;Khaled A. Abdelshafeek","doi":"10.1002/bmc.70132","DOIUrl":"https://doi.org/10.1002/bmc.70132","url":null,"abstract":"<div>\u0000 \u0000 <p>The purpose of this work was to use molecular docking techniques to examine the chemical makeup and biological activity of the petroleum ether and chloroform extracts (SPEE and CHSE, respectively) of <i>Coffee arabica</i> seeds. According to the GC/MS results of SPEE, the extract contains 28 components, which account for approximately 96% of the total. These substances fall under several categories of phytoconstituents. Nevertheless, the extract contains 28 compounds, which make up 99.1% of the total, according to the CHSE GC/MS data. Alcohols (4.93%) with 2-ethyl-1-hexanol as the primary compound, monoterpenes (3.95%) with 4-ethyl-1-octyn-3-ol accounting for 2.92%, aromatics (2.67%), esters (38.31%) with methyl palmitate as the primary (16.71%), caffeine (28.81%), hydrocarbons (10.85%) with 7-octadecene as the primary compound, and ketones (5.13%) with 2-ethyl-1-cyclohexanone as the primary (3.04%) are among them. Only <i>Bacillus subtilis</i> was susceptible to the modest antibacterial activity of both extracts. Whereas CHSE successfully targeted breast (IC₅₀ = 146.10 μg/mL) and colon cancer cells (IC₅₀ = 111.90 μg/mL), SPEE showed strong cytotoxicity against hepatocellular carcinoma (IC₅₀ = 42.41 μg/mL) and breast cancer cells (IC₅₀ = 57.76 μg/mL) in anticancer studies.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Strategy for Discovering Effective Ingredients of Lirianthe coco Flowers (Yehehua) for Anti-Insomnia Based on Molecular Networking and Network Pharmacology","authors":"Changshui Yang,&nbsp;Xia Liu,&nbsp;Fengcheng Zhang,&nbsp;Xiaoxiao Duan,&nbsp;Hongwei Zhang,&nbsp;Yanyan Zhang","doi":"10.1002/bmc.70131","DOIUrl":"https://doi.org/10.1002/bmc.70131","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Lirianthe coco</i> flowers are an alternative for <i>Albizia julibrissin</i> flowers used as anti-insomnia in Guangdong, China. They once shared the name <i>yehehua</i> (YHH) with similar cultural connotations in history. However, no comprehensive studies have been conducted to explore the effective components in YHH and the underlying mechanisms for anti-insomnia. The components in YHH were comprehensively annotated by the classical Global Natural Products Social Molecular Networking (GNPS) and Feature-Based Molecular Network (FBMN) combined with MS-DIAL to improve the accuracy of structural annotation, and the main components were labeled for network pharmacological analysis. Subsequently, the “compounds–targets–pathways” network was constructed, and molecular docking was adopted to discover effective ingredients of YHH for anti-insomnia. Finally, a total of 103 main components were annotated and summarized. Among them, lignans and flavonoids had similar or the same component category and structures as <i>A. julibrissin</i> flowers and barks. Particularly, phosphatidylcholines, previously unreported, were found from YHH. The selected effective ingredients could play synergistic roles in inflammation regulation, neuroprotection, and drug combination or assembly utilization. YHH contains effective ingredients for anti-insomnia, which can improve the treatment of insomnia. Some TCMs with common efficacy had a similar substance base.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144196985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Validated, Stability-Indicating HPLC Method for the Simultaneous Determination of Five Related Substances in Liraglutide Drug Substance","authors":"Lixiang Zhang,&nbsp;Xinyu Li,&nbsp;Jiansong Zhang,&nbsp;Xiao Wang,&nbsp;Yuqing Fu","doi":"10.1002/bmc.70118","DOIUrl":"https://doi.org/10.1002/bmc.70118","url":null,"abstract":"<div>\u0000 \u0000 <p>Liraglutide, an analog of glucagon-like peptide-1, is a treatment for the management of type 2 diabetes mellitus and obesity. In this study, we developed and validated a straightforward, sensitive, and reproducible gradient reverse-phase high-performance liquid chromatographic method for the separation and quantification of related substances in liraglutide drug substance. A C8 column was employed as the chromatographic column, with a mobile phase comprising phosphate buffer solution and acetonitrile. Under optimized chromatographic conditions, five specific impurities within liraglutide could be efficiently separated simultaneously. The resolution between main peak and each impurity was greater than 1.0, and the tailing factor of the main peak was less than 1.0. This method exhibited high sensitivity and enabled quantitative determination of both liraglutide and each impurity, thereby effectively ensuring the quality control of liraglutide products. The developed method is validated in accordance with the guidelines set by the International Conference on Harmonization regarding specificity, linearity, limit of detection, limit of quantification, accuracy, precision, and robustness.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144171534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacodynamic Material Basis and Pharmacological Mechanism of Huangqi Guizhi Wuwu Granules in Diabetic Peripheral Neuropathy","authors":"Yang Liu,&nbsp;Lele Li,&nbsp;Yan Yang,&nbsp;Xing Wang,&nbsp;Shengyu Ge,&nbsp;Lili Jin,&nbsp;Bo Feng,&nbsp;Jiao Guan,&nbsp;Heyun Zhu","doi":"10.1002/bmc.70127","DOIUrl":"https://doi.org/10.1002/bmc.70127","url":null,"abstract":"<div>\u0000 \u0000 <p>Huangqi Guizhi Wuwu Decoction (HGWD) is a traditional Chinese medicine (TCM) formula used to treat diabetic peripheral neuropathy (DPN) in China. Considering that the decoction was not easy to carry and use, we have prepared HGWD into Huangqi Guizhi Wuwu Granules (HGWG). However, the active components of HGWG that can be absorbed into plasma and its mechanism of action for DPN treatment remain unclear. In the present study, UHPLC-Q-TOF-MS and network pharmacology methods were applied to explore the potential pharmacodynamic material basis and pharmacological mechanism of HGWG for the treatment of DPN. A total of 31 absorbed components were detected in rat plasma, including 20 prototype compounds and 11 metabolites. Flavonoid-related metabolites and gingerol-related metabolites were major metabolites. The network pharmacological analysis showed that 31 absorbed constituents regulated 122 pathways through 177 targets; among them, baicalein, paeoniflorin C, and 6-gingerol were the main pharmacodynamic components of HGWG for DPN. It can be concluded that monoterpenoid glycosides, flavonoids, organic acids, gingerols, flavonoid-related metabolites, and gingerol-related metabolites were the main pharmacodynamic material basis of HGWG for DPN, and the mechanism of HGWG for DPN may be related to reducing oxidative stress and inhibiting neuroinflammation by stimulating the phosphorylation of p38 MAPK.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144171535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous Determination of Glibenclamide, Metoprolol Tartrate, and Phenol Red Using HPLC-PDA: Application in Rat Intestinal Permeability Studies and Its Greenness Assessment Using NEMI, Analytical Eco-Scale, GAPI, MoGAPI, AGREE, AGREEprep, RAPI, BAGI, and CACI","authors":"Murat Soyseven,&nbsp;Mustafa Sinan Kaynak,&nbsp;Berna Kaval,&nbsp;Mustafa Çelebier,&nbsp;Emrah Aygeyik,&nbsp;Fargana Musayeva,&nbsp;Burcu Sezgin,&nbsp;Göksel Arli","doi":"10.1002/bmc.70128","DOIUrl":"https://doi.org/10.1002/bmc.70128","url":null,"abstract":"","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability-Indicating RP-HPLC Method Development and Validation for Quantification of Butylated Hydroxyanisole Content in Loperamide Oral Solid Dosage Form","authors":"Prasanna Kumar Lankalapalli,&nbsp;Srinivasulu Kasa,&nbsp;Pranitha Sambu,&nbsp;Rama Krishna Myneni,&nbsp;Teja Kamireddy,&nbsp;Vijaykumar chollety,&nbsp;Hareesh Divadari","doi":"10.1002/bmc.70103","DOIUrl":"https://doi.org/10.1002/bmc.70103","url":null,"abstract":"<div>\u0000 \u0000 <p>The present study discusses the development of a simple, rapid, and specific high-performance liquid chromatography (HPLC) method for the estimation of butylated hydroxyanisole (BHA) in loperamide HCl capsule dosage formulations. The developed HPLC method was validated in accordance with current ICH guidelines. Chromatographic separation was achieved using a phosphate buffer (pH 6.8) as mobile phase A and a mixture of acetonitrile and buffer in a ratio of 80:20 (v/v) as mobile phase B. The flow rate was 0.8 mL/min, and column temperature was maintained at 35°C. Detection of the component was performed at 290 nm for BHA. The optimized HPLC method was validated as per current ICH guidelines and demonstrated high specificity with a linearity range of 13–83 μg/mL for BHA, with a correlation coefficient greater than 0.999. The method showed accuracy exceeding 97%. Stress studies revealed that BHA is sensitive to peroxide stress conditions. The results highlight the successful applicability of the current method for estimating BHA in marketed formulations, which can be further utilized to evaluate other formulation systems. The developed method was validated according to international ICH guidelines concerning specificity, linearity, precision, and accuracy. The method was developed and validated in a quality control lab for stability analysis.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary Metabolomics Data Reveals Lipid Metabolism and Oxidative Stress Metabolites as Potential Biomarkers for Patent Ductus Arteriosus","authors":"Mustafa Çelebier,&nbsp;Aybuke Yazici,&nbsp;Duygu Eneş,&nbsp;Abdullah Kurt,&nbsp;Bilge Başak Fidan,&nbsp;Ibrahim Ilker Cetin,&nbsp;Dilek Sahin,&nbsp;Evrim Alyamac Dizdar,&nbsp;Fatma Nur Sari","doi":"10.1002/bmc.70125","DOIUrl":"https://doi.org/10.1002/bmc.70125","url":null,"abstract":"<div>\u0000 \u0000 <p>Patent ductus arteriosus (PDA) is a common congenital heart defect in preterm infants and is associated with significant morbidity. Early diagnosis is crucial but challenging due to nonspecific clinical symptoms. This study aims to identify potential metabolomic biomarkers for early detection of PDA using human cord blood. A prospective cross-sectional study was conducted involving 45 preterm infants between 23^0/6 and 31^6/7 weeks of gestation. The diagnosis of hemodynamically significant PDA (hsPDA) was based on echocardiographic findings after 48 h, showing a left atrium-to-aortic root ratio &gt; 1.5 and/or a ductus diameter &gt; 1.5 mm. Untargeted metabolomics analysis was performed on cord blood plasma samples using quadrupole time-of-flight liquid chromatography-mass spectrometry (Q-TOF LC/MS). Data were processed for metabolites that differed between groups. Twenty infants with hsPDA formed the study group, 25 controls. Out of 4237 detected peaks, 40 showed statistically significant differences (fold change &gt; 1.5,<i>p</i> &lt; 0.05). Among these, 15 metabolites were potentially clinically relevant. Key findings included decreased levels of guanidino acetic acid, <i>S</i>-adenosylmethionine, and ceramides and increased levels of docosahexaenoic acid, arachidonic acid, and cholesterol-related molecules in the PDA group. The study reveals significant metabolic alterations in lipid metabolism and oxidative stress-related pathways in PDA infants. Further targeted metabolomics studies are warranted to validate and explore clinical applications.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Validation of the LC–MS/MS Method and Its Application for Pharmacokinetic Studies for the Simultaneous Estimation of Motixafortide and Filgrastim in rat Plasma","authors":"Lurdhu Mary K,&nbsp;Naresh Podila,&nbsp;Afzal B. Shaik,&nbsp;Jithendra Chimakurthy","doi":"10.1002/bmc.70126","DOIUrl":"https://doi.org/10.1002/bmc.70126","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Motixafortide, a CXCR4 antagonist, and filgrastim, a granulocyte colony-stimulating factor, have shown significant potential in enhancing hematopoietic stem cell mobilization and improving cancer treatment outcomes when used in combination. However, critical challenges persist due to the absence of analytical methods and the necessity for reliable quantification of both drugs in biological matrices. This research aims to address these gaps by developing and validating a liquid chromatography–tandem mass spectrometry method for the simultaneous quantification of motixafortide and filgrastim in rat plasma.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Experiment</h3>\u0000 \u0000 <p>An isocratic mobile phase with acetonitrile and buffer was employed for separation, following protein precipitation with acetonitrile, using a C₁₈ Waters X-Terra RP-18 column (150x4.6 mm,3.5 μm). Liquid chromatography–tandem mass spectrometry with an internal standard employs multiple reaction monitoring in electrospray ionization (positive ion mode) to monitor the transitions. The method was validated according to USFDA guidelines, assessing parameters such as selectivity, matrix effect, linearity, precision, accuracy, lower limit of quantification, and stability.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>This method was successfully applied in pharmacokinetic studies, ensuring reliable and accurate quantification of co-administered motixafortide and filgrastim. These findings significantly contribute to optimizing therapeutic protocols and enhancing treatment outcomes for cancer patients.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the Efficacy and Mechanism of Metabolomics and Bioinformatics on the Treatment of Qi Stagnation and Blood Stasis Coronary Heart Disease by Danshen Injection","authors":"Lei Wu,&nbsp;Mei Feng,&nbsp;You Peng,&nbsp;Yuyang Xiang,&nbsp;Rihui Liu,&nbsp;Tao Liu","doi":"10.1002/bmc.70120","DOIUrl":"https://doi.org/10.1002/bmc.70120","url":null,"abstract":"<div>\u0000 \u0000 <p>This study, grounded in traditional Chinese medicine theory, established a QZXY-CHD model to investigate DSI's impact on vascular endothelial function, inflammatory factors, oxidative stress, and myocardial energy metabolism from a holistic perspective. The results demonstrated that DSI significantly reduced serum levels of ET-1, MPO, IL-1β, TNF-α, MDA, LDH, and CK-MB, while increasing NO and SOD levels, indicating DSI's ability to inhibit inflammation, mitigate oxidative stress, and protect endothelial and organ functions. Using bioinformatics, we identified differentially expressed genes and metabolic pathways in coronary heart disease and screened diagnostic biomarkers (JDP2, ZFP36, TRAF3IP3, MRPS30, CLEC4D). Non-targeted metabolomics revealed changes in endogenous metabolites, identifying 73 differential metabolites between the control (Con) and model (Mod) groups, and 75 between the Mod and DSI groups, with 21 key overlapping metabolites. Integrated bioinformatics and metabolomics analysis highlighted critical metabolites such as arachidonic acid, leukotriene B4, linoleic acid, sucrose, D-glucose, pyroglutamic acid, and palmitic acid, as well as seven key metabolic pathways including arachidonic acid metabolism, linoleic acid metabolism, and galactose metabolism. This combined analysis provides biological evidence for identifying biomarkers of CHD host susceptibility and elucidates the mechanisms by which DSI ameliorates QZXY-CHD, offering new insights for diagnosis and disease management.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}