Biomedical Chromatography最新文献_第3页

A Novel Derivatization-Free Approach for Analysis of (+)2-Aminobutanol and Ethambutol Hydrochloride in Tablets Preparation Using RP-HPLC-NQAD

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-10 DOI: 10.1002/bmc.70049

Hao Ruan, Ying Luo, Qian Yang, Chao Liu, Yunfeng Shi, Minglong Ye, Liya Hong, Weike Su

{"title":"A Novel Derivatization-Free Approach for Analysis of (+)2-Aminobutanol and Ethambutol Hydrochloride in Tablets Preparation Using RP-HPLC-NQAD","authors":"Hao Ruan, Ying Luo, Qian Yang, Chao Liu, Yunfeng Shi, Minglong Ye, Liya Hong, Weike Su","doi":"10.1002/bmc.70049","DOIUrl":"https://doi.org/10.1002/bmc.70049","url":null,"abstract":"<div>\u0000 \u0000 <p>A novel and rapid RP-HPLC coupled with nano quantity analyte detector (NQAD) method was developed for determination of ethambutol hydrochloride and (+)2-aminobutanol in drug products. NQAD is an aerosol-based detector that can be used for the direct detection of the substance lacking ultraviolet chromophores. The two highly polar analytes were separated on a C<sub>18</sub> column with mobile phase consists of trifluoroacetic acid solution and methanol and detected with NQAD. A thorough investigation was conducted into the experimental and instrumental parameters, including the composition and ratio of mobile phase, the flow-rate of mobile phase, and nebulizer and evaporator temperature, to achieve the highest sensitivity of analytes. The method was validated as per ICH guidelines and proved to be specific, accurate, precise, linear, sensitive, and robust. The method showed adequate performance, with excellent sensitivity for limits of detection (LODs) of 5.6 and 9.8 ng for ethambutol and (+)2-aminobutanol (Impurity I), respectively. Good linearity was obtained for ethambutol and Impurity I in their concentration range both with an <i>R</i><sup>2</sup> value of 0.999. Overall, the method established in this study, utilizing a new detection technique, proved to be accurate and rapid and is applicable for directly detecting Impurity I and ethambutol in quality control.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143595233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of Anti-Biofilm Compounds From Paeoniae Radix Alba Based on Oral Biofilm Biochromatography

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-09 DOI: 10.1002/bmc.70019

Xin-Ming Liu, Yi Yu, Heng Jiang, Yu-Fei Wang, Yan Gao, Lei Xiao, Ming Liang, Jin Qi

{"title":"Screening of Anti-Biofilm Compounds From Paeoniae Radix Alba Based on Oral Biofilm Biochromatography","authors":"Xin-Ming Liu, Yi Yu, Heng Jiang, Yu-Fei Wang, Yan Gao, Lei Xiao, Ming Liang, Jin Qi","doi":"10.1002/bmc.70019","DOIUrl":"https://doi.org/10.1002/bmc.70019","url":null,"abstract":"<div>\u0000 \u0000 <p>Oral biofilms, which are known as dental plaque, are the reason for a wide range of oral and systemic diseases, which contribute to serious health risks. Paeoniae Radix Alba (PRA) is traditionally used as a folk medicine with anti-inflammatory, cardioprotective, and hepatoprotective properties. PRA is currently used in a variety of therapeutic approaches for oral diseases. Nevertheless, its inhibitory effect on oral biofilm formation and the basis for its efficacy have not been clarified. This study intended to screen the potential compounds in PRA that inhibit oral biofilm formation using biochromatography. Two biofilm models based on <i>S. mutans</i> were used to determine the inhibitory effect of PRA on biofilm formation. The extraction of PRA was divided into fractions with different polarity, the active fraction screened, and an HPLC profile constructed for the active fraction. Three potential compounds were screened using targeted oral biofilm extraction, and subsequent validation of the efficacy indicated that albiflorin is the main compound in PRA exerting anti-biofilm activity. Our results have revealed the pharmacological substance basis of PRA in inhibiting the formation of oral biofilm and provide a reference for the further use of PRA in the development of oral health products.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Quantification of Caffeic and Ferulic Acids by HPLC-UV in Nanoparticles: Degradation Profiling and Computational Peroxidation Analysis

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-06 DOI: 10.1002/bmc.70051

Igor Roesch Felix, Gabriela Wink Stringhini, Beatriz Miguel Giovanelli, Silvia Leticia Baldissera, Camila Reck Rampelotto, Tielle Moraes de Almeida, Clarissa Piccinin Frizzo, Alisson Vasques Paz, Mariana Zancan Tonel, Scheila Rezende Schaffazick, Cristiane de Bona da Silva

{"title":"Simultaneous Quantification of Caffeic and Ferulic Acids by HPLC-UV in Nanoparticles: Degradation Profiling and Computational Peroxidation Analysis","authors":"Igor Roesch Felix, Gabriela Wink Stringhini, Beatriz Miguel Giovanelli, Silvia Leticia Baldissera, Camila Reck Rampelotto, Tielle Moraes de Almeida, Clarissa Piccinin Frizzo, Alisson Vasques Paz, Mariana Zancan Tonel, Scheila Rezende Schaffazick, Cristiane de Bona da Silva","doi":"10.1002/bmc.70051","DOIUrl":"https://doi.org/10.1002/bmc.70051","url":null,"abstract":"<div>\u0000 \u0000 <p>Phenolic compounds such as ferulic acid (FA) and caffeic acid (CA) are renowned for their antioxidant and anti-inflammatory properties. However, their physicochemical instability limits broader therapeutic applications. This study aimed to develop an analytical methodology for the simultaneous quantification of these compounds in nanoparticles using liquid chromatography. The suspensions exhibited favorable characteristics, including a particle size of 117 nm, encapsulation efficiency above 60%, zeta potential of 14 mV, and adequate bioactive content. Notably, the system enhanced their photostability under UV radiation. The HPLC-UV method employed a C18 column with a mobile phase of acidic water (pH 2.6), methanol, and acetonitrile (64:18:18, v/v/v) at 1 mL/min, with detection at 223 nm, achieving fast and efficient separation. The method was linear, specific, precise, accurate, and robust, in accordance with Brazilian and ICH guidelines. Additionally, the method was stability-indicating, identifying isomeric degradation products, which represent the most common degradation reaction for these compounds. Computational analysis using density functional theory (DFT) further explored the stability of CA and FA. It revealed a strong interaction between the carboxyl group and hydrogen peroxide, suggesting reduced susceptibility to oxidation. These findings provide a foundation for designing stable formulations and enhancing the understanding of antioxidant mechanisms in phenolic compounds.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prunus mume Alleviates Hyperuricemic Renal Injury: Insights From Network Pharmacology and Experimental Models

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-05 DOI: 10.1002/bmc.70035

ShaoJun Zheng, Sheng Li, XiaoHan Diao, NaiDong Chen

{"title":"Prunus mume Alleviates Hyperuricemic Renal Injury: Insights From Network Pharmacology and Experimental Models","authors":"ShaoJun Zheng, Sheng Li, XiaoHan Diao, NaiDong Chen","doi":"10.1002/bmc.70035","DOIUrl":"https://doi.org/10.1002/bmc.70035","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Prunus mume</i> (PM), the dried flower bud of a Rosaceae plant, has a long history of use for its liver-soothing, depression-relieving, and appetite-stimulating effects. Recently, PM has gained attention for its anti-inflammatory, antioxidant, and uric acid-lowering properties. The chemical composition of PM was analyzed using network pharmacology and liquid chromatography–mass spectrometry (LC-MS). The therapeutic potential of PM for hyperuricemia-induced kidney damage was evaluated in a quail model. Antioxidant activity in an HK-2 cell model of hyperuricemia was assessed by measuring the levels of MDA, SOD, and GSH. Additionally, the anti-inflammatory potential was examined using ELISA to measure TNF-α and IL-6 levels. Western blotting was employed to study the effects on URAT1, GLUT9, and the PI3K/AKT pathway. LC-MS identified 284 compounds in PM, with 35 predicted active ingredients. The quail model demonstrated PM's protective effects on the kidneys under hyperuricemic conditions. In vitro, PM reduced oxidative stress and lowered TNF-α and IL-6 levels. It also modulated URAT1 and GLUT9 expression and influenced the PI3K/AKT pathway. PM shows promise in protecting kidneys from hyperuricemia-induced damage, likely through its anti-inflammatory and antioxidant activities, as well as the regulation of urate transport proteins and the PI3K/AKT pathway.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143554719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipidomic Analysis of Serum Lipid Profiles in Idiopathic Central Precocious Puberty and the Potential Regulatory Role of GnRHa in Lipid Metabolism

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-05 DOI: 10.1002/bmc.70029

Li-Xia Yang, Hui Chen, Yan-Hua Jiang, Hui Li, Ling-Ling Yang, Zhao Wang, Hua Shao, Jinjun Shan, Shouchuan Wang, Li-Li Lin, Ling Xu, Jin Ye

{"title":"Lipidomic Analysis of Serum Lipid Profiles in Idiopathic Central Precocious Puberty and the Potential Regulatory Role of GnRHa in Lipid Metabolism","authors":"Li-Xia Yang, Hui Chen, Yan-Hua Jiang, Hui Li, Ling-Ling Yang, Zhao Wang, Hua Shao, Jinjun Shan, Shouchuan Wang, Li-Li Lin, Ling Xu, Jin Ye","doi":"10.1002/bmc.70029","DOIUrl":"https://doi.org/10.1002/bmc.70029","url":null,"abstract":"<div>\u0000 \u0000 <p>Idiopathic central precocious puberty (ICPP) is an endocrine disorder increasingly observed in children, commonly treated with gonadotropin-releasing hormone agonists (GnRHa). However, the serum lipid profiles in girls with ICPP and the potential regulatory effects of GnRHa in lipid metabolism remain unclear. This research analyzed lipidomic profiles of serum samples from girls younger than 10 years of age, including 37 patients with ICPP, 32 ICPP patients treated with GnRHa, and 30 age-matched healthy controls. Using UHPLC-Q-Exactive Orbitrap/MS, we identified a total of 898 differential lipids in both positive and negative modes, covering classes such as phosphatidylcholine (PC, including EtherPC), acylcarnitine (CAR), triglyceride (TG), N-acyl ethanolamines (NAEs), diacylglycerol (DG), sphingomyelin (SM), phosphatidylethanolamine (PE), ceramide (Cer), and fatty acids (FAs). In the ICPP cohort, 105 lipids exhibited significant differences compared with controls, with increased levels of CAR, SM, PC, PE, TG, and FA (<i>p</i> < 0.05), and notably decreased NAE levels (<i>p</i> < 0.05). Specifically, NAE 20:1 (AUC: 1.0), NAE 18:2 (AUC: 0.948), and NAE 20:0 (AUC: 0.895) were identified as potential diagnostic biomarkers for ICPP. Following GnRHa treatment, serum peak LH and FSH levels decreased, alongside reversible changes in 44 lipids, predominantly CAR, NAE, and TG. In conclusion, this study demonstrates that ICPP is associated with significant alterations in lipid metabolism. The lipidomic changes induced by GnRHa therapy suggest a potential link between ICPP treatment and lipid homeostasis, which warrants further investigation to refine therapeutic approaches for ICPP and potentially mitigate associated metabolic disorders.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143554720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical Change of Velvet Antler After Vinegar Processing Was Related With the Increased Fecundity in Drosophila melanogaster

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-04 DOI: 10.1002/bmc.70045

Linxu Cao, Xingkang Wu, Huichun Zhao, Xiaoxia Gao, Xuemei Qin, Zhenyu Li

引用次数: 0

Novel Genotoxic Impurities in the First-Line Oral Anticoagulant Drug, Apixaban: Computational Toxicological Evaluation and Development of a Highly Sensitive LC-MS/MS Quantification Method

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-03 DOI: 10.1002/bmc.70043

Bhaskar Vallamkonda, Srinivasa Rao Yarguntla, Ranadheer Reddy Challa

{"title":"Novel Genotoxic Impurities in the First-Line Oral Anticoagulant Drug, Apixaban: Computational Toxicological Evaluation and Development of a Highly Sensitive LC-MS/MS Quantification Method","authors":"Bhaskar Vallamkonda, Srinivasa Rao Yarguntla, Ranadheer Reddy Challa","doi":"10.1002/bmc.70043","DOIUrl":"https://doi.org/10.1002/bmc.70043","url":null,"abstract":"<div>\u0000 \u0000 <p>Apixaban is an oral anticoagulant classified as a direct factor Xa inhibitor. It is widely utilized for prophylaxis and management of thromboembolic conditions, including stroke, deep vein thrombosis, and pulmonary embolism, as a primary therapeutic option. Apixaban is subjected to stringent testing for potential genotoxic impurities during its manufacturing, due to its structure indicating the possibility of such impurity's formation. Setting limits in accordance with ICH M7 guidelines is essential to reduce patient exposure and also recommended an advanced analytical tool for testing. The present study aims to establish limits based on computational toxicological evaluation, and develop and validate a highly sensitive LS-MS/MS method for quantifying three novel genotoxic impurities of apixaban, namely impurities F, G, and H. The method was established utilizing a mobile phase comprising a pH 5.5 acetate buffer and acetonitrile in a gradient mode. Used a C18 column, of 150 mm (length) × 3.0 mm (width), 2.7 μm of particle size as stationary phase. Quantification was performed with multi-response monitoring in mass spectrometry, employing precursor ions of m/z 437.2, 525.2, and 569.1 for impurities F, G, and H, respectively. The established method is validated for its intended use in accordance with regulatory guidelines and found suitable.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of Plasma Niraparib Using High-Performance Liquid Chromatography in Patients With Ovarian Cancer

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-03 DOI: 10.1002/bmc.70046

Yoshito Gando, Makoto Hoshino, Risa Ikuta, Mikio Shirota, Haruko Iwase, Takeo Yasu

{"title":"Quantification of Plasma Niraparib Using High-Performance Liquid Chromatography in Patients With Ovarian Cancer","authors":"Yoshito Gando, Makoto Hoshino, Risa Ikuta, Mikio Shirota, Haruko Iwase, Takeo Yasu","doi":"10.1002/bmc.70046","DOIUrl":"https://doi.org/10.1002/bmc.70046","url":null,"abstract":"<div>\u0000 \u0000 <p>Niraparib is a small-molecule inhibitor of poly(adenosine diphosphate [ADP]-ribose) polymerase 1/2, which is used to treat ovarian cancer. Elevated maximum blood concentrations of niraparib and the area under the blood concentration–time curve (AUC) were correlated with body weight up to 77 kg. Lower body weight increases blood niraparib concentrations and the AUC of ovarian cancer in Asian patients. Therefore, therapeutic drug monitoring (TDM) of ovarian cancer drugs may increase niraparib efficacy and minimize adverse events. In this study, we quantified niraparib in human plasma (50 μL) using a simple and specific HPLC–UV method. The analyte was separated on a reversed-phase column with an isocratic mobile phase of 0.5% KH<sub>2</sub>PO<sub>4</sub> (pH 4.5) and acetonitrile (75:25, v/v) at a flow rate of 1.0 mL/min. Calibration curves were linear over 0.25–5 μg/mL (<i>r</i><sup>2</sup> = 0.9998). Intraday and interday precision ranged from 2.25% to 6.29% and 1.73% to 3.20%, respectively, whereas accuracy and recovery ranged from −6.02% to −1.75% and > 93.2%, respectively. We cost-effectively quantified steady-state niraparib concentrations in the plasma of patients with ovarian cancer. Therefore, our method could be applied to the departments of pharmacy and clinical laboratories in general hospitals to facilitate the TDM of niraparib without the need for LC–MS/MS.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Mechanism of Kai-Xin-San to Improve Cognitive Deficits in AD Rats Induced by D-Gal and Aβ25–35 Based on Multi-Omics and Network Analysis

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-03-03 DOI: 10.1002/bmc.70047

Lifen Zhou, Min Zhang, Qin Zheng, Yonggui Song, Zhihong Yan, Huijuan Wang, Yongchang Xiong, Ying Chen, Zhinan Cai, Jinbin Yuan

{"title":"Exploring the Mechanism of Kai-Xin-San to Improve Cognitive Deficits in AD Rats Induced by D-Gal and Aβ25–35 Based on Multi-Omics and Network Analysis","authors":"Lifen Zhou, Min Zhang, Qin Zheng, Yonggui Song, Zhihong Yan, Huijuan Wang, Yongchang Xiong, Ying Chen, Zhinan Cai, Jinbin Yuan","doi":"10.1002/bmc.70047","DOIUrl":"https://doi.org/10.1002/bmc.70047","url":null,"abstract":"<div>\u0000 \u0000 <p>Alzheimer's disease (<span>AD</span>) is a common neurodegenerative disease for which there are no effective drugs. Kai-Xin-San (KXS), with definite curative effects, is widely used for the prevention and treatment of <span>AD</span> in China. But its mechanism is not yet fully understood. Based on our established rat model and previous pharmacodynamics study, Multi-omics (metabolomics, proteomics) and network analysis were integrated to explore the holistic mechanism of anti-<span>AD</span> effects of KXS. The key pathways were validated with western blot and ELISA methods. Morris water maze and Nissl staining showed that KXS could ameliorate cognitive deficits and pathological morphology of the hippocampus in <span>AD</span> rats. A total of nine metabolites were identified, which were related to pyrimidine metabolism, riboflavin metabolism, tyrosine metabolism, tryptophan metabolism, and glycerophospholipid metabolism. Proteomics results indicated that the improvement of cognitive deficits by KXS was closely related to the regulation of oxidative phosphorylation in mitochondria. Western blotting results showed that KXS significantly inhibited the expression of Mt-nd2 and Ndufb6 in <span>AD</span> rats. Integrated analysis indicated that the anti-<span>AD</span> targets of KXS were interrelated and KXS could exert its anti-<span>AD</span> effect by reducing oxidative stress, neurotoxicity, and inflammation.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143533299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Serum Pharmacochemistry and Network Pharmacology Used to Explore Potential Antidepressant Mechanisms of the Kaixin San

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-28 DOI: 10.1002/bmc.70041

Guoliang Dai, Deming Liu, Youjin Wang, Yanjun Wang, Qian Huang, Wenqing San, Xiaoyong Wang, Wenzheng Ju

{"title":"Integrated Serum Pharmacochemistry and Network Pharmacology Used to Explore Potential Antidepressant Mechanisms of the Kaixin San","authors":"Guoliang Dai, Deming Liu, Youjin Wang, Yanjun Wang, Qian Huang, Wenqing San, Xiaoyong Wang, Wenzheng Ju","doi":"10.1002/bmc.70041","DOIUrl":"https://doi.org/10.1002/bmc.70041","url":null,"abstract":"<div>\u0000 \u0000 <p>Kaixin San (KXS) is a classical prescription for the treatment of depression. However, the mechanism is not clear. In this study, serum pharmacochemistry, mediated by the UHPLC-Orbitrap Exploris 480 mass spectrometer, was used to identify compounds derived from the KXS-medicated serum. These components were used to construct a compound-target network for depression using a network pharmacology approach to predict potential biological targets of KXS. Subsequently, we established a mouse model of CUMS-induced depression and observed the antidepressant effect of KXS. The signalling pathways predicted by the network pharmacology were further validated in animal experiments. The results showed that 36 compounds were identified from the KXS-medicated serum. Based on this, 984 genes related to the compounds and 4966 genes related to depression were identified using network pharmacology. Critically, KEGG analysis identified the PI3K/Akt and NF-κB signalling pathways as the main pathways through which KXS exerts its antidepressant effect. KXS significantly alleviated depression-like behaviour and hippocampal histopathological changes in a mouse model of depression. Compared with the model group, the treatment of KXS significantly reduced the expression of protein targets in the PI3K/Akt/NF-κB signalling pathway. All these studies effectively corroborated the predicted results, confirming the feasibility of this integrated strategy.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 4","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0