Biomedical Chromatography最新文献

{"title":"UHPLC-ESI-Q-TOF-HRMS Quantitated Six C-Glycosyl Flavonoids in Oxalis corniculata and Demonstrated Their Typical In Vivo Molecular Profiling in Rats","authors":"Lijun Chen,&nbsp;Rongmei Luo,&nbsp;Wei Wang","doi":"10.1002/bmc.70622","DOIUrl":"https://doi.org/10.1002/bmc.70622","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Oxalis corniculata</i> L. exhibits neuroprotection; however, its in vivo molecular profiling remains unclear. An ultra-high performance liquid chromatography-electrospray ionization-quadrupole-time-of-flight high resolution mass spectrometry (UHPLC-ESI-Q-TOF-HRMS) was established to determine and quantify six <i>C</i>-glycosyl flavonoids in the herb, namely swertisin, schaftoside, isoschaftoside, vitexin, isovitexin, and isoorientin. They were identified and confirmed in the aqueous extract of the herb with mass error less than 5 ppm in HRMS. The founded UHPLC-ESI-Q-TOF-HRMS was optimized and validated for biological samples analysis, with coefficient of determination <i>R</i>² greater than 0.998, matrix factor being near to 1, recoveries from 85.0% to 115.0% and relative standard deviation from 0.17% to 12.88%. Their simultaneous quantification for the first time by UHPLC-ESI-Q-TOF-HRMS indicated their contents to be from 47.7 to 455.5 mg/kg in the herb in dry weight. After a single oral administration of the saturated aqueous solution of the herb extract to rats, swertisin was typically quantified in rat brain, heart, liver, kidneys, lung, spleen, small intestine, bile, and excreta under multiple reaction monitoring. Schaftoside and isoschaftoside were quantitated in rat brain. Their in vivo molecular profiling in rats was illustrated, especially their penetration of rat blood–brain barrier. Totally 17 metabolites were identified in the biological samples.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 11","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148981421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality Difference Analysis of Raw and Wine-Processed Polygonati Rhizoma Based on Correlation Between Color and Component","authors":"Yuxin Feng,&nbsp;Tao Guo,&nbsp;Yingjie Xu,&nbsp;Binrong Ge,&nbsp;Miaomiao Zhao,&nbsp;Yijun Song,&nbsp;Desheng Zhang","doi":"10.1002/bmc.70621","DOIUrl":"https://doi.org/10.1002/bmc.70621","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 &lt;p&gt;The dried rhizome of Polygonati Rhizoma, known as Huangjing, is commonly used clinically in both its raw and wine-processed forms. However, the distinctions in quality between them remain unclear, and the methods for identifying them are considerably intricate. This study aimed to analyze the differences in color and changes in key components between raw and wine-processed Polygonati Rhizoma, to reveal the correlation between the color and chemical components of Polygonati Rhizoma before and after processing, and to provide a reference for the identification and quality evaluation of Polygonati Rhizoma processed products. Based on the subjective judgment of the external properties of raw and wine-processed Polygonati Rhizoma by the eyes, an electronic eye was used to quantify their color objectively. The contents of extractum and polysaccharide were determined, and HPLC fingerprint profiles of raw and wine-processed Polygonati Rhizoma were also established to represent their chemical composition characteristics systematically. Chemometric analysis was performed using SIMCA 14.1 to evaluate their quality differences, while SPSS 27.0 and Origin software were employed for correlation analysis between color and chemical components. From raw to wine-processed Polygonati Rhizoma, the color of the samples darkened. Polysaccharide showed a decreasing trend, while extractum increased. The results of the HPLC fingerprint indicated significant chemical differences between raw and processed samples. Peaks were identified as 5-HMF (Peak 10), DDMP (Peak 12), and polygonatine A (Peak 17). By comparing the peak areas of 18 chromatographic peaks of the samples, it was revealed that from raw to processed samples, the peak areas of seven chromatographic peaks increased, one decreased, seven new components were newly generated, and three components disappeared. Based on OPLS-DA analysis of peak areas, the samples could be classified into two groups clearly; there were five chromatographic peaks with a VIP value greater than 1.0. The chromatographic peaks were numbered in order of their value as 7, 10, 17, 16, and 8; these five components were the main factors that distinguished raw and processed samples. Correlation analysis showed that the polysaccharides were positively correlated with &lt;i&gt;L&lt;/i&gt;*, &lt;i&gt;b&lt;/i&gt;*, and &lt;i&gt;E&lt;/i&gt;&lt;sub&gt;&lt;i&gt;ab&lt;/i&gt;&lt;/sub&gt;* but negatively correlated with &lt;i&gt;a&lt;/i&gt;*. Extractum and Peaks 7, 8, 10, 16, and 17 were significantly negatively correlated with &lt;i&gt;L&lt;/i&gt;*, &lt;i&gt;b&lt;/i&gt;*, and &lt;i&gt;E&lt;/i&gt;&lt;sub&gt;&lt;i&gt;ab&lt;/i&gt;&lt;/sub&gt;* but positively correlated with &lt;i&gt;a&lt;/i&gt;*. Regression analysis indicated that the contents of Peaks 7, 17, and 8, extractum, and polysaccharides could be predicted by combining the chromatic values with the regression equations, while the other components could not. This study showed that chromaticity could serve as an effective tool for predicting the component content of raw and wine-processed Polygonati Rhizoma. It w","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 11","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148981422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Hecogenin in Mouse Blood by UPLC–MS/MS and Applied to a Pharmacokinetic Study","authors":"Yujie Hu,&nbsp;Lei Zhu,&nbsp;Xueying Xiang,&nbsp;Tao Zou,&nbsp;Congcong Wen,&nbsp;Qiong Duan","doi":"10.1002/bmc.70618","DOIUrl":"10.1002/bmc.70618","url":null,"abstract":"<div>\u0000 \u0000 <p>Hecogenin, a steroidal sapogenin from agave plants, exhibits anti-inflammatory, anti-tumor, gastroprotective, and diuretic activities. A sensitive ultraperformance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for quantifying hecogenin in mouse whole blood using swertisin as internal standard. Protein precipitation with acetonitrile enabled simple pretreatment of only 20-μL blood samples. Separation was achieved on a BEH C18 column with gradient elution, and detection was performed via multiple reaction monitoring in positive electrospray ionization mode. The assay was linear over 1–2000 ng/mL (<i>r</i> = 0.9988), with a lower limit of quantification of 1 ng/mL. Intra- and interday precision (RSD) were below 11% and 12%, respectively, and accuracy ranged from 93.2% to 110.4%. Matrix effects were negligible, and extraction recovery was 87.5%–91.2%. The validated method was applied to a pharmacokinetic study in male ICR mice following intravenous (5 mg/kg) and oral (10, 20 mg/kg) administration. Noncompartmental analysis revealed low oral absolute bioavailability (10.8%–12.4%).</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LC–MS/MS Method for Simultaneous Quantification of Zolpidem, Zopiclone, and Their Metabolites in Human Urine and Its Pharmacokinetic Application","authors":"Varsha Chauhan,&nbsp;Manu Sharma,&nbsp;Murtada Taha,&nbsp;Manish Kumar,&nbsp;Shruti Srivastava,&nbsp;Naiyer Shahzad,&nbsp;Md Ali Mujtaba,&nbsp;Ameeduzzafar Zafar","doi":"10.1002/bmc.70612","DOIUrl":"10.1002/bmc.70612","url":null,"abstract":"<div>\u0000 \u0000 <p>Zolpidem (Z1) and zopiclone (Z2) are widely prescribed for insomnia; but their misuse in drug-facilitated crimes presents significant forensic and clinical challenges. This study aimed to develop and validate a simple and sensitive LC–MS/MS method, coupled with dispersive liquid–liquid microextraction (DLLME), for the simultaneous determination of Z1, Z2, and 18 phase I and phase II metabolites (20 species) in human urine. DLLME employed 1 mL of dichloromethane as extractant and 2 mL of acetonitrile as disperser per 2 mL of urine, reducing halogenated-solvent use compared with conventional liquid–liquid extraction. Chromatographic separation was achieved on a HyPURITY C18 column with water–acetonitrile (70:30, v/v) containing 0.2% formic acid. Z1 and Z2 were validated according to ICH guidelines over 0.1–200 ng/mL, showing linearity (<i>R</i>² &gt; 0.999), accuracy (96.29%–99.56%), precision (RSD ≤ 3.12%), and recovery (96.12%–98.74%). Because authentic metabolite standards were unavailable, metabolite concentrations were estimated using parent-drug calibration curves and are considered semi-quantitative. In five healthy volunteers, hydroxylated and carboxylated metabolites peaked at 2–12 h and remained detectable up to 84 h. These findings support the method's potential for retrospective urinary screening of Z-drug exposure in forensic and clinical toxicology.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Simplified and Robust LC–MS/MS Method for Simultaneous Quantification of Six Steroid Hormones Associated With Polyendocrine Metabolic Ovarian Syndrome","authors":"Mengxue Feng,&nbsp;Yan Song,&nbsp;Xueqing Qian","doi":"10.1002/bmc.70609","DOIUrl":"10.1002/bmc.70609","url":null,"abstract":"<div>\u0000 \u0000 <p>Polyendocrine metabolic ovarian syndrome (PMOS), formerly known as polycystic ovary syndrome (PCOS), is characterized by dysregulation of multiple steroid hormones. Accurate quantification of these analytes is essential for laboratory evaluation; however, currently may involve complex sample-preparation workflows or provide insufficient analytical coverage, particularly for dehydroepiandrosterone sulfate (DHEAS). A liquid chromatography–tandem mass spectrometry (LC–MS/MS) assay was established for the simultaneous measurement of six steroid hormones associated with PMOS. Sample preparation combined liquid–liquid extraction with protein precipitation. Chromatographic separation was completed within 6 min. Validation followed international recommendations and included linearity, sensitivity, precision, accuracy, matrix effects, selectivity, and specificity. All analytes showed excellent linearity (<i>R</i> = 0.9959–0.9995), with intra- and inter-day CVs ≤ 5.6%. Spike recovery ranged from 85.2% to 114.9%. Accuracy was further supported by third-party QC materials and successful participation in an external quality assessment program. The assay provided an expanded linear range for DHEAS (20–20,000 ng/mL). This validated LC–MS/MS assay provides rapid, sensitive, and reproducible quantification of six PCOS-related steroid hormones and is suitable for routine laboratory implementation.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148905033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous Determination of Three Residual Monomers in Polyvinyl Alcohol Eye Drops Using a Dual-Wavelength RP-HPLC Method and Evaluation of Its Greenness","authors":"Yang Zeyan,&nbsp;Gao Wenfen,&nbsp;Li Zhiyuan,&nbsp;Ma Xiaobin,&nbsp;Li Yongfei","doi":"10.1002/bmc.70613","DOIUrl":"10.1002/bmc.70613","url":null,"abstract":"<div>\u0000 \u0000 <p>Polyvinyl alcohol eye drops contain high-molecular-weight polymeric raw materials and excipients whose synthesis, storage, and pharmaceutical manufacturing processes may introduce or generate residual monomers, thereby affecting the quality, safety, and stability of the formulation. Therefore, establishing a reliable analytical method for the simultaneous determination of multiple residual monomers is essential for improving the quality control of such ophthalmic formulations. In this study, a dual-wavelength reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the simultaneous quantitative determination of 2-pyrrolidone, <i>N</i>-vinyl-2-pyrrolidone (NVP), and vinyl acetate (VA) in polyvinyl alcohol eye drops. Chromatographic separation was achieved on a NanoChrom 300 C18 column using an isocratic mobile phase consisting of water, methanol, and acetonitrile (90:5:5, v/v/v), with detection at 205 and 235 nm. The greenness and environmental sustainability of the proposed method were comprehensively evaluated using three complementary assessment tools: the Analytical Eco-Scale, the Analytical Method Greenness Score (AMGS), and the Modified Green Analytical Procedure Index (MoGAPI). The results demonstrated that the method is simple and provides satisfactory sensitivity, accuracy, and precision, making it suitable for the determination and quality control of the target residual compounds in ophthalmic formulations containing povidone as an excipient.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using UPLC–MS/MS and LC–MS/MS to Investigate the Pharmacokinetics in SD Rat Plasma and Tissue Distribution in KM Mice of Daphnoretin Nanomicelles","authors":"Zhi-min Lv,&nbsp;Ju Zhang,&nbsp;Guo Feng,&nbsp;Xiang-ling Qu,&nbsp;Ting-ting Liu,&nbsp;Xin-ping Wei,&nbsp;Li Zhang,&nbsp;Shi-qiong Qin,&nbsp;Yun-li Wei,&nbsp;Gang Liu,&nbsp;Ke-xin Ma,&nbsp;Jin-xin Hou,&nbsp;Wei Li,&nbsp;Yi Sui","doi":"10.1002/bmc.70614","DOIUrl":"10.1002/bmc.70614","url":null,"abstract":"<div>\u0000 \u0000 <p>Daphnoretin (DAP) has various pharmacological activities, but its in vivo disposition after nanomicellar formulation remains unclear. This study compared the pharmacokinetics and tissue distribution of free DAP and two polymeric nanomicellar formulations, PP-DAP and GA-DAP, following intravenous administration. Plasma DAP concentrations in rats were determined by UPLC–MS/MS, and DAP concentrations in mouse tissues were determined by LC–MS/MS. Compared with free DAP, PP-DAP and GA-DAP showed higher systemic exposure, longer apparent elimination half-lives, and lower apparent clearance. The AUC_0–∞ values of DAP, PP-DAP, and GA-DAP were 5474.14, 11,211.04, and 15,019.86 h · ng/mL, respectively, and the corresponding <i>T</i>_1/2 values were 6.84, 9.24, and 9.90 h. DAP was detected in the heart, liver, spleen, lung, and kidney, with the nanomicellar formulations showing altered tissue distribution and GA-DAP exhibiting relatively sustained hepatic distribution. These findings suggest that nanomicellar formulation alters the in vivo disposition of DAP. However, free and micelle-associated DAP were not separately quantified, precluding direct characterization of in vivo drug release, and the validation range of the tissue quantification method was limited. Further studies are warranted to evaluate the in vivo behavior and liver-directed delivery potential of GA-DAP.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148905103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetic and Tissue Distribution Analysis of Scutellarin and Iso-Scutellarin From Breviscapine Injection in Rats by a Validated UPLC-MS/MS Method","authors":"Min Wen,&nbsp;Lijuan Fan,&nbsp;Lipan Yang,&nbsp;Qi Zhang,&nbsp;Xiaobo Lv","doi":"10.1002/bmc.70608","DOIUrl":"10.1002/bmc.70608","url":null,"abstract":"<div>\u0000 \u0000 <p>Breviscapine injection (BI), a standardized botanical drug for cardiovascular and cerebrovascular diseases, lacks adequate characterization of its pharmacokinetics and tissue distribution. A UPLC-MS/MS method was developed and validated for simultaneous quantification of scutellarin and its metabolite iso-scutellarin in rat plasma and tissues after intravenous BI administration. Sample preparation used methanol protein precipitation, with detection via positive electrospray ionization MRM. Validation followed the Chinese Pharmacopoeia and bioanalytical PK guidance. The assay showed LLQs of 10.0 ng/mL (scutellarin) and 5.0 ng/mL (iso-scutellarin), good linearity (<i>r</i>² ≥ 0.990), precision (RSD ≤ 11.1%), accuracy (91.7%–106.8%), and acceptable recovery and matrix effects. PK analysis revealed moderate-to-rapid systemic elimination of scutellarin. Both analytes distributed to tissues within 0.25 h postdose, with exposure ranking: bladder &gt; small intestine &gt; kidney &gt; small intestine &gt; kidney &gt; stomach &gt; liver &gt; plasma &gt; heart &gt; skeletal muscle &gt; pancreas &gt; skin &gt; fat &gt; lung &gt; ovary &gt; testis &gt; adrenal &gt; heart &gt; skeletal muscle &gt; pancreas &gt; thymus &gt; spleen &gt; brain. The method is suitable for BI studies. The limited brain penetration suggests formulation optimization to prolong plasma exposure may be worth exploring, and the relatively high hepatic distribution points to a potential need for monitoring liver and kidney function.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stable Isotope-Resolved Metabolomics in Elucidating Mitochondrial Metabolic Reprogramming and Therapeutic Targets.","authors":"Dan Yang, Xinyan Wu, Xiufang Luo, Yuanyin Teng, Xiyue Duan, Tingting Kang, Wenze Wu","doi":"10.1002/bmc.70560","DOIUrl":"10.1002/bmc.70560","url":null,"abstract":"<p><p>Stable isotope-resolved metabolomics (SIRM) has emerged as a pivotal methodology for dissecting mitochondrial metabolic reprogramming and its connection to therapeutic targets. By combining isotopically labeled substrates with metabolic flux analysis (MFA), SIRM enables dynamic, quantitative tracking of carbon flux through mitochondrial pathways. This review focuses on three disease contexts where SIRM has provided transformative insights: cancer, neurodegenerative disorders (Alzheimer's and Parkinson's diseases), and metabolic syndrome (type 2 diabetes and non-alcoholic fatty liver disease). Recent applications have delineated reprogramming signatures, identified metabolic dependencies, and elucidated drug mechanisms. SIRM also facilitates therapeutic monitoring and resistance assessment, offering quantitative biomarkers for patient stratification. We summarize recent advances, technical challenges, AI-powered innovations, and future directions, providing a foundation for optimizing metabolism-targeted therapies.</p>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 9","pages":"e70560"},"PeriodicalIF":1.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13426047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148629426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Profiling and Pharmacological Evaluation of Dichloromethane Extract From Aerial Parts of Ferula ferulaeoides for Antithrombotic, Anti-Inflammatory, and Analgesic Activities","authors":"Lina Liu,&nbsp;Qinghai Dong,&nbsp;Qi Zheng,&nbsp;Jieyu Shi,&nbsp;Shusen Liu,&nbsp;Zhihui Wang,&nbsp;Min Wang,&nbsp;Jianyong Si,&nbsp;Jingxue Ye,&nbsp;Wenlan Li,&nbsp;Junchi Wang,&nbsp;Guibo Sun","doi":"10.1002/bmc.70568","DOIUrl":"10.1002/bmc.70568","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aims to elucidate the chemical composition of the dichloromethane extract from the aerial parts of <i>Ferula ferulaeoides</i> (DEAFF) and to explore its biological activities. The composition of DEAFF was analyzed by UHPLC-Q-Orbitrap-MS/MS. Then, the carrageenan-induced tail thrombosis model, xylene-induced ear swelling model, and acetic acid-induced writhing model were established to evaluate the relevant activities of DEAFF. Network pharmacology research and molecular docking were conducted to elucidate the pathways and key targets underlying the multiple activities of DEAFF. A total of 30 chemical constituents were tentatively identified in DEAFF based on UHPLC-Q-Orbitrap-MS/MS analysis. Pharmacodynamic results showed that DEAFF significantly reduced the proportion of thrombus in the tail of mice, effectively alleviated ear swelling, improved the degree of ear tissue lesions, and significantly reduced the number of writhing movements in mice, indicating that it has multiple pharmacological effects such as antithrombosis, anti-inflammation, and analgesia. Network pharmacology studies have shown that multiple pathways such as the PI3K-Akt signaling pathway and the MAPK signaling pathway play a crucial role in the pharmacological effects of DEAFF. In this study, the chemical components of DEAFF were preliminarily identified, and its effects on thrombosis prevention, anti-inflammation, and analgesia were systematically investigated.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148872823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}