Detoxication Strategy of Aflatoxins in Coix Seed Based on Stir-Frying Processing and LC–ESI–MS/MS Quantification

IF 1.7 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2025-09-01 DOI:10.1002/bmc.70213

Yongsong Zhai, Chujun Tao, Keyu Zhang, Feng Qiu

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Abstract

Coix seed is easy to be contaminated with aflatoxins. To find out its detoxification strategy and explore its storage environment affecting the contents of AFB₁, AFB₂, AFG₁, and AFG₂, 23 batches of coix seed samples were collected, in which nine batches of samples were selected to make mildewed by the method of artificial cultivation and natural mycotoxin production. These coix seed samples were further processed with plain-frying and bran-frying, respectively. Commercial coix seed samples had a high probability of containing AFTs with a detection rate of 87.0% and a rejection rate of 30.4%. The contents of AFTs in coix seed samples could be significantly decreased by both plain-frying and bran-frying processing. Compared with plain-frying, bran-frying processing can reduce the contents of AFTs in coix seed more effectively. Therefore, promoting the use of bran-frying processing will help reduce mycotoxin pollution in the food.

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基于炒法和LC-ESI-MS /MS定量的薏苡仁黄曲霉毒素脱毒策略

薏苡仁容易被黄曲霉毒素污染。为了解其解毒策略，探讨其贮藏环境对AFB1、AFB2、AFG1和AFG2含量的影响，采集23批薏苡仁样品，选取9批样品采用人工培养和天然产霉毒素的方法进行霉变处理。对这些薏苡仁样品分别进行了平炒和煎炸处理。商品薏米种子样品含有AFTs的概率较高，检出率为87.0%，拒绝率为30.4%。薏苡仁样品中AFTs的含量经平炒和煎炸处理均显著降低。与平炒工艺相比，煎炸工艺能更有效地降低薏苡仁中AFTs的含量。因此，推广使用油炸加工将有助于减少食品中的霉菌毒素污染。

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.