Biomedical Chromatography最新文献

{"title":"Chemical Profile and Quantitative Comparison of Compounds of Different Botanical Parts of Pogostemon cablin From Different Geographical Regions","authors":"Yameng Zhu,&nbsp;Huizi Ouyang,&nbsp;Zhoujing Feng,&nbsp;Linchang Fan,&nbsp;Xiunan Cao,&nbsp;Yanxu Chang,&nbsp;Jun He","doi":"10.1002/bmc.70201","DOIUrl":"10.1002/bmc.70201","url":null,"abstract":"<div>\u0000 \u0000 <p>In the present study, a comprehensive study on the chemical constituents of <i>Pogostemon cablin</i> (PC) based on plant metabolomics was conducted. A total of 72 nonvolatile and 72 volatile chemical components of PC were characterized by ultra-high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS) and gas chromatography–mass spectrometry (GC–MS), respectively. Subsequently, 15 nonvolatile and 14 volatile chemical components were identified as potential markers for discriminating between different botanical parts of PC. Furthermore, 20 major compounds of PC were quantitatively determined by ultra-high-performance liquid chromatography–mass spectrometry (UPLC-MS/MS) and the GC–MS method. Notably, the content of pogostone in the aerial part was the highest. Conversely, the content of other components in the leaves was relatively high, with patchouli alcohol being the highest. This study elucidates in detail the metabolic analysis of different botanical parts of PC and provides a reliable reference for its development and rationalization.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144891703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhus chinensis Mill Derived Anti-Allergic Ingredients Screening Using MAS-Related G Protein-Coupled Receptor X2 Cell Membrane Chromatography","authors":"Jinjin Wang,&nbsp;Na Guo,&nbsp;Quan Zhang,&nbsp;Runpeng Gu,&nbsp;Yanni Lv","doi":"10.1002/bmc.70203","DOIUrl":"10.1002/bmc.70203","url":null,"abstract":"<div>\u0000 \u0000 <p>Mas-related G-protein coupled receptor member X2 (MrgprX2) serves as a potential therapeutic target for pseudoallergic reactions. Cell membrane chromatography (CMC) is an efficient and reliable technique for screening active components from complex systems, which can be used to search for MrgprX2 antagonists in herbs. Therefore, we used a previously constructed MrgprX2-HALO-tag/CMC-HPLC-MS system specifically for identifying natural antagonists. <i>Rhus chinensis</i> Mill has been found to possess anti-inflammatory activities, but its active components targeting MrgprX2 remain unexplored. In this study, the MrgprX2-HALO-tag/CMC-HPLC-MS method successfully identified fisetin as the first reported MrgprX2-targeting component from <i>Rhus chinensis</i> Mill. Through frontal analysis, we demonstrated that fisetin exhibits strong binding affinity (<i>K</i>_<i>D</i> = 2.02 μM) with MrgprX2, representing the first quantitative assessment of this interaction. Molecular docking revealed four key binding residues (TRP248, ASP184, LEU247, and GLU164) that form stable interactions with fisetin. Pharmacological validation using LAD2 cells confirmed fisetin's potent anti-allergic activity via MrgprX2 antagonism. In summary, these findings not only identify fisetin as an active constituent of <i>Rhus chinensis</i> Mill but also further confirm that it targeted MrgprX2 to exert anti-inflammatory properties, offering new perspectives for developing anti-pseudoallergic therapeutics.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144891767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-Free HPLC Method to Evaluate Neuraminidase Activity in Biological Fluids Containing the Newcastle Disease Virus","authors":"Siddharth Neog,&nbsp;Sachin Kumar,&nbsp;Vishal Trivedi","doi":"10.1002/bmc.70205","DOIUrl":"10.1002/bmc.70205","url":null,"abstract":"<div>\u0000 \u0000 <p>Newcastle disease virus utilizes its multifunction hemagglutinin neuraminidase (HN) protein for sialic acid recognition and its cleavage from the adjacent lactose unit. Detection of neuraminidase activity of HN is crucial for studying NDV infection biology. Traditional neuraminidase assays predominantly employ synthetic fluorogenic substrates such as 2′-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid. However, existing methods are limited by their inability to accurately mimic the natural environment of glycan substrates. Although the majority of neuraminidase assays in current use have been optimized for influenza neuraminidases, methods specifically developed and validated for HN of NDV are limited. Here, we present an optimized high-performance liquid chromatography-based protocol tailored for NDV-HN protein that utilizes an Aminex HPX-87H carbohydrate column for analyzing enzyme activity and sialollactose as a physiologically relevant substrate. Upon enzymatic cleavage by hemagglutinin-neuraminidase protein, free sialic acid, cleaved from the sialollactose moiety, is directly detected at 210 nm, bypassing the need for derivatization with chromogenic or fluorogenic agents. With the help of standard curves, the quantity of sialic acid released can be efficiently measured under different conditions such as at different pH or in the presence of an inhibitor. It could be crucial for studies evaluating viral infectivity, drug efficacy, and mutant phenotypes of the NDV-HN protein.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144891762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “A LC–MS/MS Method for the Simultaneous Determination of 6-Cyanodopamine, 6-Nitrodopamine, 6-Nitrodopa, 6-Nitroadrenaline and 6-Bromodopamine in Human Plasma and Its Clinical Application in Patients With Chronic Kidney Disease”","authors":"","doi":"10.1002/bmc.70207","DOIUrl":"10.1002/bmc.70207","url":null,"abstract":"&lt;p&gt;\u0000 &lt;span&gt;Ribeiro, LF&lt;/span&gt;, &lt;span&gt;Babadopulos, T&lt;/span&gt;, &lt;span&gt;Oliveira, M&lt;/span&gt;, &lt;span&gt;Nishimaru, F&lt;/span&gt;, &lt;span&gt;Zatz, R&lt;/span&gt;, &lt;span&gt;Motta, R&lt;/span&gt;, &lt;span&gt;Moraes, O&lt;/span&gt;, &lt;span&gt;Moraes, E&lt;/span&gt;, &lt;span&gt;Peterson, LW&lt;/span&gt;, &lt;span&gt;De Nucci, G&lt;/span&gt;. &lt;span&gt;A LC–MS/MS Method for the simultaneous Determination of 6-Cyanodopamine, 6-Nitrodopamine, 6-Nitrodopa, 6-Nitroadrenaline, and 6-Bromodopamine in Human Plasma and Its Clinical Application in Patients With Chronic Kidney Disease&lt;/span&gt;, &lt;i&gt;Biomedical Chromatography&lt;/i&gt;. &lt;span&gt;2024&lt;/span&gt; Volume &lt;span&gt;38&lt;/span&gt;, Issue &lt;span&gt;8&lt;/span&gt; https://doi.org/10.1002/bmc.5896.\u0000 &lt;/p&gt;&lt;p&gt;We identified two errors that need to be corrected in the manuscript. The mobile phases A and B are inverted, and in the sample extraction procedure, it is written that a wash and sample dilution were made with ammonium acetate, which in reality were made with deionized water.&lt;/p&gt;&lt;p&gt;In paragraph 8 of “2.5 Extraction procedure” section, the text in the second sentence: “Ammonium acetate (200 mm, 0.5 ml) was added, and the sample was homogenized under vortex for 20 s” was incorrect. This should have read: “Deionized H&lt;sub&gt;2&lt;/sub&gt;O (0.5 ml) was added, and the sample was homogenized under vortex for 20 s”.&lt;/p&gt;&lt;p&gt;Also in paragraph 8 of “2.5 Extraction procedure” section, the text in the fifth sentence: “The samples (1 ml) were applied to the SPE cartridges followed by washing with 200 mm ammonium acetate (1.5 ml).” was incorrect. This should have read: “The samples (1 ml) were applied to the SPE cartridges followed by washing with deionized water (1.5 ml).”&lt;/p&gt;&lt;p&gt;In the Abstract, the text in the second sentence: “… perfused with 82% mobile phase A (acetonitrile–H&lt;sub&gt;2&lt;/sub&gt;O; 90:10, v/v) + 0.4% acetic acid and 18% mobile phase B (deionized H&lt;sub&gt;2&lt;/sub&gt;O)+ 0.2% formic acid …” was incorrect. This should have read: “… perfused with 82% mobile phase A (deionized H&lt;sub&gt;2&lt;/sub&gt;O)+ 0.2% formic acid and 18% mobile phase B (acetonitrile–H&lt;sub&gt;2&lt;/sub&gt;O; 90:10, v/v) + 0.4% acetic acid …”.&lt;/p&gt;&lt;p&gt;In paragraph 9 of “2.6 Quantification of Catecholamines” section, the text in the second sentence: “… perfused by 82% of mobile phase A (acetonitrile–H2O; 90:10, v/v) + 0.4% acetic acid and 18% mobile phase B (deionized H2O) + 0.2% formic acid …” was incorrect. This should have read: “… perfused by 82% of mobile phase A (deionized H2O) + 0.2% formic acid and 18% mobile phase B (acetonitrile–H2O; 90:10, v/v) + 0.4% acetic acid …”.&lt;/p&gt;&lt;p&gt;In the caption of fig. 4, of “3.3 LC-MS/MS analysis” section, the second sentence of the caption: “Chromatographic conditions: mobile phase A, 90:10 (v/v) acetonitrile/water + 0.4% acetic acid; mobile phase B, water + 0.2% formic acid; isocratic flow, composed of 82% mobile phase A and 18% mobile phase B;” was incorrect. This should have read: “Chromatographic conditions: mobile phase A, water + 0.2% formic acid; mobile phase B, 90:10 (v/v) acetonitrile/water + 0.4% acetic acid","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/bmc.70207","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144888232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amelioration of Guishao Yigong Decoction on Colorectal Cancer Through the Integration of 16S rRNA Sequencing and Fecal Metabolomics","authors":"Wenwen Zhou,&nbsp;Yuwen Fan,&nbsp;Xiaoxiao Zhang,&nbsp;Meijuan Liu,&nbsp;Shu Jiang,&nbsp;Erxin Shang,&nbsp;Jinao Duan","doi":"10.1002/bmc.70198","DOIUrl":"10.1002/bmc.70198","url":null,"abstract":"<div>\u0000 \u0000 <p>Guishao Yigong Decoction (GYD), a classical formula, has been used to treat colorectal cancer (CRC) in clinical practices. However, its mechanism is still unclear. Increasing evidence suggests that the gut microbiota may serve as a potential target for treating CRC. Therefore, this study aims to elucidate the amelioration and potential mechanism of GYD on CRC by comprehensively analyzing the metagenome of gut microbiota and fecal metabolome. The results indicated that GYD significantly reduced the number and size of adenomas in the mouse colon, decreased spleen index, alleviated mouse emaciation and rectal bleeding, and protected the colonic barrier. 16S rRNA gene sequencing analysis revealed that GYD could markedly improve the dysbiosis of gut microbiota in CRC mice, increasing the abundance of beneficial bacteria and decreasing the abundance of pathogenic bacteria. Furthermore, the disordered fecal metabolic profiling of CRC mice was notably reversed by GYD. Following GYD administration, metabolites such as thiamine pyrophosphate, 3-methylpentanoic acid, and propanoic acid significantly increased, whereas 2-hydroxy-2-methylpropanoic acid, levodopa, and stearic acid remarkably decreased. Correlation analysis further indicated a close relationship between differential gut microbiota and metabolites. In conclusion, the amelioration of GYD on CRC might involve the regulation of gut microbiota and its metabolism.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Multimodal Strategy for Quality Control and Botanical Origin Traceability of Codonopsis Radix based on HPLC Fingerprints With Chemometric Analysis","authors":"Yalan Li,&nbsp;Piaopiao Yang,&nbsp;Huixian Qing,&nbsp;Zhiyan Xie,&nbsp;Kexin Zhou,&nbsp;Lili Sun,&nbsp;Meiling Chen,&nbsp;Meng Wang,&nbsp;Xiaoliang Ren","doi":"10.1002/bmc.70185","DOIUrl":"10.1002/bmc.70185","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Codonopsis</i> Radix (CR) is widely used in the food and pharmaceutical industries. However, the existence of three botanical origins in CR makes it challenging to ensure the quality of CR in the market. In this study, a method was developed to identify and analyze CR from different botanical origins, and their signature components were quantitatively compared. HPLC fingerprints of 55 CR batches demonstrated similarities ranging from 0.707 to 0.994. Chemometrics was used to analyze the different botanical origins of CR and identify significant components affecting their classification. Hierarchical cluster analysis (HCA) and principal component analysis (PCA) indicated that 55 CR batches could be divided into three groups according to their botanical origins. Partial least squares discriminant analysis (PLS-DA) was applied to confirm classification results and obtain the chemical markers. In addition, the reliability of the markers was revalidated by counter propagation artificial neural networks (CP-ANN). The content determination results showed significant differences in the contents of adenosine, eleutheroside B, tangshenoside I, and lobetyolin in the three kinds of CR. The methods and results of this study provide a reliable basis for the identification and rational utilization of CR.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Validation of HPTLC Fingerprint Method for 16 Homeopathic Mother Tinctures Lacking Standards","authors":"Arun Kumar,&nbsp;Amit Pandey,&nbsp;Narendra Kumar Gaur,&nbsp;Sunil Kumar Vishwakarma,&nbsp;Snigdha Suman Dalua,&nbsp;Poorva Tiwari,&nbsp;Ramachandran Valavan","doi":"10.1002/bmc.70194","DOIUrl":"10.1002/bmc.70194","url":null,"abstract":"<div>\u0000 \u0000 <p>High performance thin layer chromatography (HPTLC) is an important quality control method for homeopathic mother tinctures. Homeopathic Pharmacopoeia of India (HPI) provides standards for thin layer chromatography for many drugs. This study is aimed to develop HPTLC standards for those drugs for which TLC standards are not available in HPI. Sixteen drugs (<i>Abrom. r</i>., <i>Bapt</i>., <i>Cass. fis</i>., <i>Cydo. vul</i>., <i>Dict. alb</i>., <i>Guat. gaum</i>., <i>J. ashok</i>., <i>Lactuc</i>., <i>Laur</i>., <i>Liat</i>., <i>Neg. ame</i>., <i>Olib</i>., <i>Onon. spi</i>., <i>Ruta</i>, <i>Chirata</i>, and <i>Ver. anth</i>.) were identified for which TLC method is not available. The design of experiments (DOE) was formulated to determine solvents in the mobile phase, application quantity and dilution, and spray reagent with different combinations to develop separated chromatograms/fingerprints. The developed mobile phases were (9:10) in 11 out of 16 drugs, (80:10:10) in 2 out of 16 drugs, (40:40:10), (50:35:15), (100:15), and (100:11:11:26) are each in one drug. Spray reagent anisaldehyde was found compatible in 13 out of 16 drugs, and (NP/PEG) was used in 3 out of 16 drugs. Daylight, 254 nm, and 366 nm were used as light sources. HPTLC standards for 16 homeopathic drugs lacking entries in HPI were successfully developed. Continuous development of analytical standards is essential to support quality assurance in homeopathic formulations.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Analysis and Bioactivity Evaluation of Phoenix dactylifera L. Ethanolic Extract and Cosmetic Cream: Antibacterial, Antioxidant, and Anti-Inflammatory Properties","authors":"Inasse Cherfi,&nbsp;Abir Benaissa,&nbsp;Nasma Mahboub,&nbsp;M. Taher Benmoussa,&nbsp;Inas Ahmadi,&nbsp;Brahim Dif,&nbsp;Ikram Djaber","doi":"10.1002/bmc.70199","DOIUrl":"10.1002/bmc.70199","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Phoenix dactylifera</i> L. (Ghars variety) seeds, widely available in Algeria, represent a valuable natural resource. Their rich phytochemical composition presents promising potential for applications in health and skincare. This study evaluates the phytochemical profile and biological activities of ethanolic extracts (EE) from Ghars date seeds and assesses the antimicrobial efficacy of a formulated cosmetic cream. Ethanolic extraction yielded 11.425% EE. Phytochemical screening, total flavonoid content (TFC), and total phenolic content (TPC) were determined. Antioxidant activity was evaluated using ferric reducing antioxidant power (RAP) and DPPH assays. Anti-inflammatory activity was assessed via membrane stabilization, and the antimicrobial potential of the EE-based cream was tested against selected bacterial strains. The EE exhibited high flavonoid (68.81 mg/mL) and phenolic content (118.6 mg/mL). Antioxidant assays showed strong activity, with IC₅₀ values of 47.86 μg/mL (FRAP) and 35.85 μg/mL (DPPH). The extract demonstrated notable anti-inflammatory effects. The EE-based cream displayed significant antimicrobial activity, with inhibition zones up to 27 mm against <i>Bacillus subtilis</i> and marked effects against <i>Staphylococcus aureus</i> and <i>Pseudomonas aeruginosa</i>. Ghars date seed EE shows strong antioxidant, anti-inflammatory, and antimicrobial properties, supporting its use as a natural ingredient in cosmetic and healthcare formulations.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144861619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Profiling and Dual In Vitro In Vivo Antidiabetic Assessment of Ethyl Acetate Fraction and Essential Oil From Allium ascalonicum","authors":"Aftab Ahmad Khan,&nbsp;Fazal Ur Rahman,&nbsp;Madeeha Shabnam,&nbsp;Fida Hussain,&nbsp;Muhammad Saeed Jan,&nbsp;Atta Ur Rahman,&nbsp;Eman R. Elsharkawy","doi":"10.1002/bmc.70193","DOIUrl":"10.1002/bmc.70193","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Allium ascalonicum</i> (shallot) is a traditional medicinal plant recognized for its rich phytochemical content and therapeutic potential. This study aimed to evaluate the phytochemical composition, antidiabetic activity, and toxicity profile of the ethyl acetate fraction and essential oil. GC–MS analysis identified 34 compounds in the ethyl acetate fraction, with major constituents including phenol, 2-methoxy-4-vinyl- (17.77%) and 2-cyclohexene-1-one (7.99%), while the essential oil revealed 10 major compounds such as hexadecanoic acid, methyl ester (30.77%), and caryophyllene oxide (10.82%). Phytochemical screening confirmed the presence of flavonoids, alkaloids, tannins, saponins, and terpenoids. Heavy metal analysis indicated acceptable levels, with lead and cobalt undetected. In vitro antidiabetic assays demonstrated potent α-amylase, DPP-4, and PTP-1B inhibitory activity, especially by the essential oil (IC₅₀ = 7.93, 7.74, and 17.86 μg/mL), comparable with standard drugs. Acute toxicity studies revealed no adverse effects up to 2000 mg/kg. In vivo, both the ethyl acetate fraction and essential oil significantly reduced fasting blood glucose (over 40%), improved lipid profiles, and prevented weight loss in streptozotocin-induced diabetic mice. Further studies focusing on molecular targets and signaling pathways are warranted to fully elucidate the therapeutic potential of <i>A. ascalonicum</i> in diabetes management.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144861621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Novel Immunoprecipitation Method for Extracting Monoclonal Antibodies From Brain Tissue and Its Application to Assessing In Vivo Brain Penetration in Mouse via Liquid Chromatography–Mass Spectrometry","authors":"Sangsoo Hwang,&nbsp;Seo-jin Park,&nbsp;Jeong-hyeon Lim,&nbsp;Young G. Shin","doi":"10.1002/bmc.70196","DOIUrl":"10.1002/bmc.70196","url":null,"abstract":"<p>A novel extraction method was developed to quantify monoclonal antibodies from brain tissue, crucial for pharmacokinetic assessments. We focused on optimizing detergent use for compatibility with liquid chromatography–mass spectrometry analysis. IGEPAL and sodium deoxycholate were selected for their ability to preserve antibody integrity during extraction, with conditions optimized to a 4% concentration. This was most effective for maintaining antibody structure and maximizing recovery. Pharmacokinetic analysis was conducted on the 8D3 monoclonal antibody, known for its brain penetration abilities. Administered at 20 mg/kg, its concentration in the brain was measured using the optimized extraction methods. The 4% SDC method showed superior extraction efficiency, significantly enhancing brain exposure levels with a higher maximum concentration and area under the curve compared to the 4% IGEPAL and detergent-free methods. This highlights the importance of detergent selection in accurate pharmacokinetic measurements. We also addressed potential blood contamination. Specific perfusion conditions effectively removed 98% of blood contaminants, ensuring that pharmacokinetic measurements reflected true monoclonal antibody levels in brain tissue. Overall, this study establishes a robust method for monoclonal antibody extraction from brain tissue, enhancing the analysis of brain-targeted therapies and providing a valuable framework for future studies aiming to measure drug concentrations in brain tissue.</p>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/bmc.70196","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}