Biomedical Chromatography最新文献

{"title":"Preclinical Concomitant Toxicokinetic Study of Schisandrin B by HPLC–MS/MS","authors":"Sanwen Li,&nbsp;Qing Shao,&nbsp;Hongqun Qiao","doi":"10.1002/bmc.70068","DOIUrl":"https://doi.org/10.1002/bmc.70068","url":null,"abstract":"<div>\u0000 \u0000 <p>Schisandrin B (Sch B), a natural lignan extracted from schisandra chinesis, has exhibited various pharmacological activities including anticancer effects. However, studies on the preclinical toxicokinetic profile of Sch B have not been publicly reported. This study aimed to investigate the preclinical concomitant toxicokinetics of multiple administration of Sch B. Sch B was administered orally to rats and dogs at 150, 300, and 600 mg/kg/day and 50, 100, and 200 mg/kg/day, respectively, for 26 weeks. Plasma concentrations of Sch B were determined by a validated HPLC–MS/MS method. According to the toxicokinetic results, significant gender differences were observed in the rats, and females had higher exposures than males for each dosing group. Toxicokinetic analysis demonstrated a notable accumulation in the plasma of dogs during the repeated administration of Sch B, and the degree of accumulation increased with the increase of the dose. The findings of this study indicated that there were differences in the concomitant toxicokinetics of Sch B between rats and dogs. These results can inform clinical studies and provide valuable insights for future human Sch B risk assessments.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiomics Analyses of Citrus aurantium L. Var. Amara and Ginger Reveals Lipid Metabolism, Bile Acid Biosynthesis, and Gut Microbiome Rebalance Supporting Their Anti-Obesity Effects","authors":"Ying Yang,&nbsp;Wentao Shao,&nbsp;Huiyun Shu,&nbsp;Ping Wang,&nbsp;Yi Tao","doi":"10.1002/bmc.70034","DOIUrl":"https://doi.org/10.1002/bmc.70034","url":null,"abstract":"<div>\u0000 \u0000 <p>Both the flower of <i>Citrus aurantium</i> L. var. amara (CAVA) and rhizome of <i>Zingiber officinale Roscoe</i> (ginger) are food and medicinal homologous plants that have been used in China for aiding gastric digestion and preventing obesity. However, the combinatorial use of the two plants on obesity remains elusive. Our endeavor aimed to identify the optimal synergistic ratio between CAVA and ginger and to explore the underlying mechanism of their anti-obesity effects. Aqueous CAVA and ginger extracts were prepared separately and then combined into nine different ratios. The constituents of CAVA and ginger were unambiguously characterized by employing LC–MS. High-fat diet (HFD)–induced obese C57BL/6J mice were established and then administered with the nine combinations of CAVA-G extracts for 6 weeks. The trajectory of mice's body weights was analyzed. Besides, hematoxylin and eosin (HE) staining of the liver and oil red O staining of adipose tissue were performed. ELISA assay was employed to measure serum levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C). Moreover, serum metabolic profiling was conducted through UPLC-Q-TOF/MS analysis. Gut microbiota analysis was performed via 16S rRNA gene sequencing. Pattern recognition and Pearson correlation analysis were used to pinpoint the key endogenous metabolites and microbiota. Two groups of CAVA-G combination treatment (C3 and A1) significantly prevented the increase of weight in mice. According to our analysis, the best anti-obesity effect was achieved when the ratio between CAVA and ginger was 37:63. The levels of TC and LDL-C were dramatically decreased in the C3 group, whereas the level of TG was significantly reduced in the A1 group. Interestingly, HDL-C level was increased dramatically in the C3 group. Compared with the model group, a total of 16 and 25 biomarkers were identified for groups C3 and A1, respectively. These biomarkers are mainly implicated in lipid metabolism and primary bile acid biosynthesis. Interestingly, the abnormal diversity of gut microbiota was induced by HFD feeding. Treatment with C3 or A1 significantly increased the relative abundance of <i>Akkermansia</i> and <i>Novosphingobium</i>, while reducing the relative abundance of <i>Dorea</i>, <i>Bacteroides</i> and <i>Roseburia</i>. Of note, this is the first report that <i>Novosphingobium</i> is involved in preventing obesity. These findings will layer a foundation for the usage of CAVA-G for preventing obesity.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and Application of Time-Resolved Fluorescence Strip Method for Biotin Detection in Milk Powder","authors":"Jialin Hu,&nbsp;Yongwei Feng,&nbsp;Qi Zhou,&nbsp;Ting Yang,&nbsp;Qianqian Lu,&nbsp;Chuanlai Xu,&nbsp;Lingling Guo","doi":"10.1002/bmc.70057","DOIUrl":"https://doi.org/10.1002/bmc.70057","url":null,"abstract":"<div>\u0000 \u0000 <p>Biotin can be added to milk powder to achieve recommended daily intake levels. In order to determine whether the actual amount of biotin added in food is consistent with the labeled content, a time-resolved fluorescence immunochromatographic assay (TRFICA) was established based on monoclonal antibodies for biotin detection in milk powder with a detection limit of 33.70 μg/kg, a relative recovery rate of 98.43%–100.38%, and a coefficient of variation of 3.36%–10.77%. After comparing the detection results of the two methods, we found that the proposed TRFICA results were consistent with the microbiological quantification method for vitamins, thus confirming the accuracy of the method. The detection time of the TRFICA was about 15 min, demonstrating rapid on-site detection and quantification of biotin concentrations in milk powder.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143698870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Mechanism of Action of Honeybran-Fried Cimicifuga Rhizoma in the Treatment of IBS-D Based on Metabolomics and Network Pharmacology","authors":"Jing Zhu,&nbsp;Yuxuan Zou,&nbsp;Yigeng Wu,&nbsp;Xiaoxia Deng,&nbsp;Yiming Huang,&nbsp;En Yuan,&nbsp;Qi Chen","doi":"10.1002/bmc.70026","DOIUrl":"https://doi.org/10.1002/bmc.70026","url":null,"abstract":"<div>\u0000 \u0000 <p>Honeybran-fried Cimicifuga Rhizoma (HBCR) is often used to treat prolonged diarrhea and prolapse of the anus, uterine prolapse, and gastric ptosis caused by spleen qi deficiency and the inability to elevate qi, and thus the lowering of middle qi. Rats were divided randomly into four groups. Fecal samples of rats in each group were subjected to metabolomics analysis. We identified the chemical components of HBCR using liquid chromatography–tandem mass spectrometry. We predicted the potential active components and key targets of HBCR using network pharmacology to construct a “drug–potential active ingredient–target–disease” network. The key targets screened by network pharmacology and differential metabolites screened by metabolomics analysis were subjected to combined pathway analysis. Pharmacodynamic indices showed that HBCR had a good therapeutic effect upon IBS-D. Metabolomics analysis revealed 26 differential metabolites in the treatment of IBS-D by HBCR. A total of 69 chemical components were identified, and 32 potential active components and 296 key targets were screened. Combination of metabolomics analysis and network pharmacology for joint pathway analysis revealed that the therapeutic effect of HBCR may be affected by the metabolism of linoleic acid, retinol, arachidonic acid, and tryptophan. HBCR had significant therapeutic effects in rats with IBS-D.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Cooperative Strategy of Hippocampus Lipidomics and Anti-Inflammatory Analysis to Evaluate the Antidepressant Effect of Zhi-Zi-Chi Decoction on CUMS Mice","authors":"Chuan Chai,&nbsp;Bo Jin,&nbsp;Tong Xie,&nbsp;Yuhan Cui,&nbsp;Xiaobing Cui,&nbsp;Chenxiao Shan,&nbsp;Sheng Yu,&nbsp;Hongmei Wen","doi":"10.1002/bmc.70058","DOIUrl":"https://doi.org/10.1002/bmc.70058","url":null,"abstract":"<div>\u0000 \u0000 <p>In this study, Balb/c mice were subjected to chronic unpredictable mild stress (CUMS) and treated with Zhi-zi-chi Decoction (ZZCD). Using a hippocampal lipidomics approach that combined ultra performance liquid chromatography (UPLC)-Q-Exactive Orbitrap MS with multivariate statistical techniques and targeted metabolic pathway analysis, we identified potential lipid metabolites and pathways associated with depression. Meanwhile, anti-inflammatory analyses were conducted in the hippocampus of mice. The chromatograms revealed that most lipids of the same class eluted within the same time period. In the scatter plot, the control and CUMS groups were obviously separated, whereas the ZZCD-treated or fluoxetine-treated groups were positioned between them. In positive and negative ion modes, a comprehensive screening identified 130 differential lipid metabolites, which were classified into 5 groups and 17 types. ZZCD was hypothesized to have a certain call-back efficiency for some differential lipid metabolites. The study identified three target metabolic pathways with certain influence values: glycerophosphate metabolism, linoleic acid metabolism, and α-linolenic acid metabolism. Although ZZCD's inhibitory effect on IL-6 was not significant, it demonstrated good therapeutic effects in reducing central system inflammation associated with IL-1β and TNF-α. The research suggested that the pathogenesis of depression might be closely related to lipid metabolism. ZZCD exhibited antidepressant effects by regulating endogenous lipid metabolism in CUMS mice.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myocardial Lipidomics Revealed Glycerophospholipid and Sphingolipid Metabolism as Therapeutic Targets of Qifu Decoction Against Heart Failure","authors":"Xuemei Su,&nbsp;Xin Ding,&nbsp;Junli Liang,&nbsp;Lei Zhang,&nbsp;Yang Zhang,&nbsp;Yan Qiao,&nbsp;Hongrui Ma,&nbsp;Ya Zhang,&nbsp;Yuping Tang,&nbsp;Guangguo Tan","doi":"10.1002/bmc.70063","DOIUrl":"10.1002/bmc.70063","url":null,"abstract":"<div>\u0000 \u0000 <p>Qifu decoction (QFD) has shown potential benefits in treating heart failure. However, the potential mechanism of QFD remains unclear. In this study, myocardial lipidomics, based on ultra-high-performance liquid chromatography coupled with an electrospray ionization hybrid quadrupole Orbitrap mass spectrometry (UPLC-ESI-Q-Exactive/MS), was employed to identify potential therapeutic targets of QFD for treating heart failure in a mice model induced by ligating the left anterior descending coronary artery. It was found that 47 lipid metabolites were associated with heart failure, of which 35 showed a significant reversal during QFD treatment. The QFD-reversed lipid metabolites were mainly located on phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, and ceramide, which were involved in glycerophospholipid and sphingolipid metabolism. The results of Western blotting analysis revealed that QFD could effectively alleviate heart failure through increasing the levels of lysophosphatidylcholine acyltransferase 1 (LPCAT1) and sphingomyelin synthase 1 (SMS1) and reducing the levels of acid sphingomyelinase (aSMase) and phospholipase A2 (PLA2) to regulate the metabolic disorders of glycerophospholipid and sphingolipid metabolism. All these results could be concluded that glycerophospholipid and sphingolipid metabolism were the two crucial target pathways for QFD against heart failure, which laid the theoretical groundwork for its clinical application.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the Molecular Mechanism of XiaoXianXiong Decoction in the Treatment of Atherosclerosis Based on UHPLC-Q Exactive Focus MS/MS, Network Pharmacology, and Experimental Validation","authors":"Hou Yafang,&nbsp;Lu Chenxi,&nbsp;Zhang Haoran,&nbsp;Liu Yuan,&nbsp;Ren Feifei,&nbsp;Du Xia,&nbsp;Chen Zhiyong","doi":"10.1002/bmc.70062","DOIUrl":"10.1002/bmc.70062","url":null,"abstract":"<div>\u0000 \u0000 <p>Atherosclerosis (AS), a leading pathological basis of severe cardiovascular diseases, poses a significant threat to human health. XiaoXianXiong Decoction (XXXD), a classical traditional Chinese medicine (TCM) prescription, has demonstrated promising effects in the treatment of AS. To investigate the underlying mechanism of XXXD in treatment with AS, we used UHPLC-Q Focus MS/MS, network pharmacology and in vivo validation methods. The results showed that 59 chemical components of XXXD were identified. Network pharmacology showed that 11 key compounds, 10 key targets and five key signaling pathways involved in the therapeutic effects of XXXD on AS. Experimental verification confirmed that XXXD significantly improved dyslipidemia, lipid accumulation and pathological changes in the aorta during AS. These effects were linked to the inhibition of the PI3K/AKT signaling pathway, down-regulation of PI3K, HRAS, EGF, CREB and up-regulation of NOS3 expression. This work may provide a theoretical basis for further research on the molecular mechanisms for XXXD in AS treatment.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying of Anticoagulant Ingredients From Moutan Cortex Based on Spectrum-Effect Relationship Analysis Combined With GRA, PLS, and SVM Algorithms","authors":"Weijie Pan,&nbsp;Qianru Chen,&nbsp;Menghua Wu,&nbsp;Yue Sun,&nbsp;Ming Li,&nbsp;Shumei Wang,&nbsp;Xingyang Xue,&nbsp;Jiang Meng","doi":"10.1002/bmc.70060","DOIUrl":"https://doi.org/10.1002/bmc.70060","url":null,"abstract":"<div>\u0000 \u0000 <p>Moutan Cortex (MC) is a renowned Chinese medicine used for promoting blood circulation and removing blood stasis. However, the active ingredients are unclear. This study aimed to identify and validate the active ingredients of MC. UPLC fingerprints of 23 batches of MC from various origins were analyzed. The activating blood efficacy of MC was assessed by evaluating the inhibitory effects on thrombin and factor Xa (FXa) using the chromogenic substrate method. Active ingredients were identified through spectrum-effect relationship analysis using gray relation analysis (GRA), partial least squares (PLS), and support vector machine (SVM) algorithms. Consequently, five components were identified as potential active ingredients: mudanpioside H, oxypaeoniflorin, 1,2,3,4,6-penta-O-galloyl-β-d-glucose (PGG), benzoyloxypaeoniflorin, and suffruticoside A/B/C/D. The pharmacological activities of these five active ingredients were further confirmed by measuring their thrombin inhibition ability and antithrombotic effects in zebrafish, and their interactions with thrombin and FXa were examined using molecular docking technology. Oxypaeoniflorin, benzoyloxypaeoniflorin, and PGG demonstrated significant efficacy in promoting blood circulation and resolving blood stasis, as well as strong binding affinities. This study provides a biochemical foundation for the anticoagulant effects of MC and offers valuable insights for quality control and the development of novel anticoagulant ingredients drugs.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Performance Liquid Chromatography–Ultraviolet Assay for the Determination of Pirtobrutinib Levels in a Patient With Mantle Cell Lymphoma","authors":"Yoshito Gando,&nbsp;Takeo Yasu,&nbsp;Mari Shimoda,&nbsp;Masao Tukada","doi":"10.1002/bmc.70061","DOIUrl":"https://doi.org/10.1002/bmc.70061","url":null,"abstract":"<div>\u0000 \u0000 <p>Pirtobrutinib is a Bruton's tyrosine kinase inhibitor used to treat mantle cell lymphoma and chronic lymphocytic leukemia. Pirtobrutinib has a steady-state trough concentration of &gt; 825 ng/mL, corresponding to a 90% inhibitory concentration of Bruton's tyrosine kinase. Therefore, maintaining stable trough concentrations of pirtobrutinib is clinically important; however, no methods of monitoring pirtobrutinib levels have been developed. In this study, our aim was to develop a method to determine pirtobrutinib levels in human plasma and validate it for therapeutic drug monitoring. Pirtobrutinib and ibrutinib (internal standard) were separated on a reversed-phase column using a mobile phase comprising 0.5% KH₂PO₄ (pH 4.5) and acetonitrile (52:48, v/v) at a flow rate of 1.0 mL/min. Ultraviolet detection was performed at 234 nm. Calibration curves for pirtobrutinib were linear (<i>r</i>² = 0.9998) in the range of 0.25–10 μg/mL. The intraday and interday validation coefficients were 0.72%–2.86% and 1.29%–3.22%, respectively. This study is the first one to develop and validate a method for quantifying pirtobrutinib in human plasma. These findings may support the widespread application of therapeutic drug monitoring for pirtobrutinib.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolite Identification of Huangqi Guizhi Wuwu Granules in Rat Urine and Feces Based on Ultra–High-Performance Liquid Chromatography With Quadrupole Time-of-Flight Mass Spectrometry","authors":"Yang Liu,&nbsp;Yan Yang,&nbsp;Xing Wang,&nbsp;Shengyu Ge,&nbsp;Lele Li,&nbsp;Bo Feng,&nbsp;Lili Jin,&nbsp;Jiao Guan,&nbsp;Heyun Zhu","doi":"10.1002/bmc.70059","DOIUrl":"https://doi.org/10.1002/bmc.70059","url":null,"abstract":"<div>\u0000 \u0000 <p>Huangqi Guizhi Wuwu Decoction (HGWD), a famous Chinese medicine prescription, has been widely used in the treatment of diabetic peripheral neuropathy and scleroderma in China. Considering that the decoction was not easy to store and carry, our research group has converted HGWD into Huangqi Guizhi Wuwu Granules (HGWG) in the early stage. However, the in vivo metabolism profile of HGWG was still unclear. In this study, an ultra–high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) method was established to identify prototypes and metabolites in urine and feces of rats after oral administration of HGWG. A total of 100 compounds were identified in urine and feces samples. In the urine samples, 28 prototype compounds and 41 related metabolites were identified. In the feces samples, 39 prototype compounds and 12 related metabolites were identified. Monoterpenoid glucosides, flavonoids, organic acids, gingerols, and terpenes were the main prototype compounds in both rat urine and feces. Flavonoid-related metabolites, organic acid-related metabolites, and gingerol-related metabolites were the major metabolites in rat urine, and flavonoid-related metabolites were the major metabolites in rat feces. This study can provide a theoretical basis for the drug metabolism and new drug development research of HGWG.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}