Biomedical Chromatography最新文献

{"title":"Preliminary Metabolomics Data Reveals Lipid Metabolism and Oxidative Stress Metabolites as Potential Biomarkers for Patent Ductus Arteriosus","authors":"Mustafa Çelebier,&nbsp;Aybuke Yazici,&nbsp;Duygu Eneş,&nbsp;Abdullah Kurt,&nbsp;Bilge Başak Fidan,&nbsp;Ibrahim Ilker Cetin,&nbsp;Dilek Sahin,&nbsp;Evrim Alyamac Dizdar,&nbsp;Fatma Nur Sari","doi":"10.1002/bmc.70125","DOIUrl":"https://doi.org/10.1002/bmc.70125","url":null,"abstract":"<div>\u0000 \u0000 <p>Patent ductus arteriosus (PDA) is a common congenital heart defect in preterm infants and is associated with significant morbidity. Early diagnosis is crucial but challenging due to nonspecific clinical symptoms. This study aims to identify potential metabolomic biomarkers for early detection of PDA using human cord blood. A prospective cross-sectional study was conducted involving 45 preterm infants between 23^0/6 and 31^6/7 weeks of gestation. The diagnosis of hemodynamically significant PDA (hsPDA) was based on echocardiographic findings after 48 h, showing a left atrium-to-aortic root ratio &gt; 1.5 and/or a ductus diameter &gt; 1.5 mm. Untargeted metabolomics analysis was performed on cord blood plasma samples using quadrupole time-of-flight liquid chromatography-mass spectrometry (Q-TOF LC/MS). Data were processed for metabolites that differed between groups. Twenty infants with hsPDA formed the study group, 25 controls. Out of 4237 detected peaks, 40 showed statistically significant differences (fold change &gt; 1.5,<i>p</i> &lt; 0.05). Among these, 15 metabolites were potentially clinically relevant. Key findings included decreased levels of guanidino acetic acid, <i>S</i>-adenosylmethionine, and ceramides and increased levels of docosahexaenoic acid, arachidonic acid, and cholesterol-related molecules in the PDA group. The study reveals significant metabolic alterations in lipid metabolism and oxidative stress-related pathways in PDA infants. Further targeted metabolomics studies are warranted to validate and explore clinical applications.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Validation of the LC–MS/MS Method and Its Application for Pharmacokinetic Studies for the Simultaneous Estimation of Motixafortide and Filgrastim in rat Plasma","authors":"Lurdhu Mary K,&nbsp;Naresh Podila,&nbsp;Afzal B. Shaik,&nbsp;Jithendra Chimakurthy","doi":"10.1002/bmc.70126","DOIUrl":"https://doi.org/10.1002/bmc.70126","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Motixafortide, a CXCR4 antagonist, and filgrastim, a granulocyte colony-stimulating factor, have shown significant potential in enhancing hematopoietic stem cell mobilization and improving cancer treatment outcomes when used in combination. However, critical challenges persist due to the absence of analytical methods and the necessity for reliable quantification of both drugs in biological matrices. This research aims to address these gaps by developing and validating a liquid chromatography–tandem mass spectrometry method for the simultaneous quantification of motixafortide and filgrastim in rat plasma.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Experiment</h3>\u0000 \u0000 <p>An isocratic mobile phase with acetonitrile and buffer was employed for separation, following protein precipitation with acetonitrile, using a C₁₈ Waters X-Terra RP-18 column (150x4.6 mm,3.5 μm). Liquid chromatography–tandem mass spectrometry with an internal standard employs multiple reaction monitoring in electrospray ionization (positive ion mode) to monitor the transitions. The method was validated according to USFDA guidelines, assessing parameters such as selectivity, matrix effect, linearity, precision, accuracy, lower limit of quantification, and stability.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>This method was successfully applied in pharmacokinetic studies, ensuring reliable and accurate quantification of co-administered motixafortide and filgrastim. These findings significantly contribute to optimizing therapeutic protocols and enhancing treatment outcomes for cancer patients.</p>\u0000 </section>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the Efficacy and Mechanism of Metabolomics and Bioinformatics on the Treatment of Qi Stagnation and Blood Stasis Coronary Heart Disease by Danshen Injection","authors":"Lei Wu,&nbsp;Mei Feng,&nbsp;You Peng,&nbsp;Yuyang Xiang,&nbsp;Rihui Liu,&nbsp;Tao Liu","doi":"10.1002/bmc.70120","DOIUrl":"https://doi.org/10.1002/bmc.70120","url":null,"abstract":"<div>\u0000 \u0000 <p>This study, grounded in traditional Chinese medicine theory, established a QZXY-CHD model to investigate DSI's impact on vascular endothelial function, inflammatory factors, oxidative stress, and myocardial energy metabolism from a holistic perspective. The results demonstrated that DSI significantly reduced serum levels of ET-1, MPO, IL-1β, TNF-α, MDA, LDH, and CK-MB, while increasing NO and SOD levels, indicating DSI's ability to inhibit inflammation, mitigate oxidative stress, and protect endothelial and organ functions. Using bioinformatics, we identified differentially expressed genes and metabolic pathways in coronary heart disease and screened diagnostic biomarkers (JDP2, ZFP36, TRAF3IP3, MRPS30, CLEC4D). Non-targeted metabolomics revealed changes in endogenous metabolites, identifying 73 differential metabolites between the control (Con) and model (Mod) groups, and 75 between the Mod and DSI groups, with 21 key overlapping metabolites. Integrated bioinformatics and metabolomics analysis highlighted critical metabolites such as arachidonic acid, leukotriene B4, linoleic acid, sucrose, D-glucose, pyroglutamic acid, and palmitic acid, as well as seven key metabolic pathways including arachidonic acid metabolism, linoleic acid metabolism, and galactose metabolism. This combined analysis provides biological evidence for identifying biomarkers of CHD host susceptibility and elucidates the mechanisms by which DSI ameliorates QZXY-CHD, offering new insights for diagnosis and disease management.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison and Characterization of Polysaccharides From Various Sources and Before and After Honey Processing of Astragali Radix Utilizing Saccharide Mapping","authors":"Aoli Shang,&nbsp;Yadong Li,&nbsp;Ying Zhao,&nbsp;Jun Chen","doi":"10.1002/bmc.70121","DOIUrl":"https://doi.org/10.1002/bmc.70121","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aimed to investigate the differences in polysaccharides in Astragali Radix (AR) from different sources, as well as before and after honey processing, through the analysis of enzymatic hydrolysates. The hydrolysis product of astragalus polysaccharides (APS) by β-galactosidase was characterized using hydrophilic interaction ultra-high-performance liquid chromatography coupled with time-of-flight mass spectrometry (HILIC-UHPLC-QTOF/MS). The results indicated that all oligosaccharide fragments were composed of hexoses, with sugar groups linked by 1,6-glycosidic bonds. APS enzymatic hydrolysates fingerprints were established using hydrophilic interaction high-performance liquid chromatography with an evaporative light scattering detector (HILIC-HPLC-ELSD) and comprehensively evaluated based on their similarity. Semi-quantitative analysis of each enzymatic hydrolysate was performed, combined with partial least squares discriminant analysis (PLS-DA) and significance testing to further analyze the main differential sugar fragments of AR in different growth modes and before and after honey processing. Glucose and oligosaccharides with degree of polymerization (DP) of 5 and 6 were identified as indicators to distinguish different growth modes. Glucose can serve as an indicator to distinguish raw AR from honey-processed AR (HAR). This study identified quality evaluation indicators of APS based on saccharide mapping, providing a reference for the development of quality standards for AR.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Integrated Strategy Based on Metabolomics and Pharmacodynamics to Study the Mechanism of Action of Curcumin on the Animal Model of Dysmenorrhea","authors":"Zhimin Chen,&nbsp;Jie Wu,&nbsp;Fuli Hu,&nbsp;Mingyue Ao,&nbsp;Ying Peng,&nbsp;Yu Huang,&nbsp;Kaipei Luo,&nbsp;Changjiang Hu","doi":"10.1002/bmc.70124","DOIUrl":"https://doi.org/10.1002/bmc.70124","url":null,"abstract":"<div>\u0000 \u0000 <p>Curcumin is a natural food additive with significant biological activity, which is getting more and more attention in treating dysmenorrhea. But the underlying mechanism of action remains unclear. The objective of this study is to evaluate the therapeutic effect of curcumin on dysmenorrhea rats and reveal the underlying mechanism by metabolomics and pharmacodynamics. The dysmenorrhea rats were constructed by chronic unpredictable stress plus isolation feeding and subcutaneous injection of estradiol benzoate and oxytocin. The results demonstrate that curcumin can inhibit the writhing response, regulate liver function indexes such as ALT and AST to normal levels, significantly reverse the levels of AVP and PEG2, promote the stability of hormone levels in the body, and repair damaged uterine tissue. Moreover, 75 differential metabolites were screened, among which curcumin significantly regulated 19 differential metabolites related to the treatment of dysmenorrhea, involving five metabolic pathways including phenylalanine, tryptophan, and tryptophan biosynthesis, phenylalanine metabolism, etc. Curcumin has a good therapeutic effect on dysmenorrhea, which may be related to its anti-inflammatory and hepatoprotective effects and regulation of a variety of metabolic pathways, which highlights the broad application prospect of curcumin in the treatment of dysmenorrhea.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144140669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and Systematic Characterization of Chemical Constituents in Astragali radix–Curcumae rhizoma Herb Pair Using an UPLC-QTOF-MS–Based Strategy","authors":"Qi-Rui He,&nbsp;Ya-Cong Chen,&nbsp;Ming-Xia Lu,&nbsp;Rui-Qian Sun,&nbsp;Sha-Sha Wang,&nbsp;Xue Qiu,&nbsp;Qian-Lu Ma,&nbsp;Chang-Yin Li,&nbsp;Xi-Ying Tan","doi":"10.1002/bmc.70097","DOIUrl":"https://doi.org/10.1002/bmc.70097","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Astragali radix</i>–<i>Curcumae rhizoma</i> (AC), a traditional Chinese herb pair, has been extensively used in clinical practice for the treatment of various types of cancer. However, there is a paucity of literature that comprehensively elucidates the chemical constituents of AC. The complex composition of AC remains poorly understood, with many constituents yet to be fully distinguished. Therefore, a strategy was established for identifying and characterizing the chemical constituents of AC based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). Furthermore, the exploratory quantitative analysis was conducted on its representative compounds using a calibration curve method. The results showed that a total of 140 compounds were identified, including 40 flavonoids, 50 sesquiterpenes, 15 amino acids, 13 saponins, 6 organic acids, 4 curcumins, 4 nucleosides, and 8 other compounds. Among the 24 compounds determined, calycosin-7-glucoside was found to have the highest content. In conclusion, this study has developed a reliable qualitative strategy and an exploratory quantitative method and systematically characterized the chemical constituents in AC, which lays a solid foundation for quality control of AC, as well as for the further screening of potential effective constituents.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of 25-Hydroxyvitamin D3 in Rat Brain by Derivatization-Assisted LC/ESI-MS/MS","authors":"Toma Shibuya,&nbsp;Fuwari Shishikura,&nbsp;Natsuki Yoshida,&nbsp;Shoujiro Ogawa,&nbsp;Tatsuya Higashi","doi":"10.1002/bmc.70119","DOIUrl":"https://doi.org/10.1002/bmc.70119","url":null,"abstract":"<p>Recent studies have suggested that vitamin D deficiency may have relations with various neuropsychiatric diseases as well as bone diseases. However, the concentrations of vitamin D metabolites in the brain and the relationship between their brain and serum concentrations remain poorly understood. To answer these questions, we developed and validated an LC/ESI-MS/MS method for quantifying 25-hydroxyvitamin D₃ [25(OH)D₃], an established marker for assessing vitamin D sufficiency/deficiency, in the rat brain and compared the brain concentrations with the serum concentrations. To enhance the assay sensitivity and specificity, the 25(OH)D₃ was derivatized with 4-[4-(1-pipelidinyl)phenyl]-1,2,4-triazoline-3,5-dione (PIPTAD) after purification of the brain sample by a two-step solid-phase extraction. A good linearity was obtained within the range of 20–1000 pg/g tissue, and the intra-assay and interassay precision and accuracy were acceptable. In normal rats (<i>n</i> = 6), the brain 25(OH)D₃ concentrations ranged from 128 to 175 pg/g tissue, which were extremely low (approximately 1/100) compared to the serum concentrations. The bile duct ligation caused the decreased serum 25(OH)D₃ level, which produced the subsequent decreased brain 25(OH)D₃ level (44–79 pg/g, <i>n</i> = 6). These results strongly suggested that the serum 25(OH)D₃ concentration has a significant effect on its brain level.</p>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bmc.70119","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144117972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple Reaction Monitoring LC-MS/MS Quantification of N-Nitrosamine Drug Substance Related Impurities of Clonidine in Drug Substance and in Tablet Dosage Form","authors":"Bhaskar Vallamkonda,&nbsp;Srinivasa Rao Yarguntla,&nbsp;Ranadheer Reddy Challa","doi":"10.1002/bmc.70123","DOIUrl":"https://doi.org/10.1002/bmc.70123","url":null,"abstract":"<div>\u0000 \u0000 <p>N-nitrosamine drug substance related impurities (NDSRIs) present recent concerns for manufacturers of both drug substances and products. The regulatory authorities prioritize understanding the chemical properties, potential formation, and control methods of these impurities due to their adverse effects. Clonidine is a medication for hypertension and attention deficit hyperactivity disorder, characterized by a secondary amine in its structure and a propensity to generate NDSRIs. Its chemical structural evaluation reveals a possible risk for the formation of two separate NDSRIs, namely, N-nitroso and N-dinitroso clonidine. The carcinogenic/mutagenic category was established using carcinogenic potency categorization approach method, and the corresponding acceptable intake values were implemented. A liquid chromatography–tandem mass spectrometry method in a multiple reaction monitoring mode was established to quantify both impurities in its drug substance and tablet dosage. A Poroshell C18 column of 150 × 4.6 mm, and with particle size of 2.7 μm was employed at a temperature of 30°C. A gradient elution method opted with water and acetonitrile as the mobile phases, containing formic acid as a buffering agent. Method validation conducted as per regulatory standards and demonstrated superior specificity, linearity, accuracy, precision, and robustness. As a result, this method is regarded as suitable for its intended purpose.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144117971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetic Study of Eight Bioactive Components of Buyang Huanwu Decoction in LPS Induced Chronic Inflammatory and Control Rats Using UPLC-MS/MS","authors":"Li Jiang,&nbsp;Yanling Xiong,&nbsp;Weiwei Wang,&nbsp;Yuting Zheng,&nbsp;Liping Huang,&nbsp;Yuhui Liu,&nbsp;Guoliang Xu","doi":"10.1002/bmc.70117","DOIUrl":"https://doi.org/10.1002/bmc.70117","url":null,"abstract":"<div>\u0000 \u0000 <p>Inflammation plays a pivotal role in the pathogenesis of numerous chronic inflammatory diseases, including cardiovascular diseases. Buyang Huanwu decoction (BYHWD) is widely employed to treat chronic inflammation and related disorders. However, the pharmacokinetics of BYHWD's active compounds under inflammatory conditions remain unclear. This study aims to investigate the comparative pharmacokinetics of eight bioactive components in control and LPS-induced chronic inflammatory rats following oral administration of BYHWD. A chronic inflammatory rat model was established by administering low-dose lipopolysaccharide (LPS, 200 μg<b>/</b>kg) via tail vein injection. An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) method was developed for simultaneous quantification of eight major bioactive components of BYHWD in rat plasma. Subsequently, the pharmacokinetic profiles of these analytes were characterized in both control and inflammatory rats. Excellent linearity (<i>R</i>² &gt; 0.9958) was observed for all analytes in rat plasma samples. Absolute recoveries ranged from 50.84% to 111.86%, and matrix effects varied between 85.04% and 110.14%. The method met the criteria for intra- and inter-day precision, accuracy, and stability. In conclusion, the established UPLC-MS/MS method reliably investigated the pharmacokinetic characteristics of BYHWD's bioactive components and indicate that chronic inflammation may reduce the absorption of these compounds, providing valuable insights into BYHWD's clinical applications.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144108986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Natural Deep Eutectic Solvents' Extraction Process From Atractylodes macrocephala–Astragali Radix Using the Dual Mathematical Model and Its Activity Evaluation","authors":"Wenlong Huang,&nbsp;Qingling Meng,&nbsp;Mingjiang Mao,&nbsp;Boxuan Wang,&nbsp;Chenhuan Shentu,&nbsp;Xiaohong Li,&nbsp;Jie Hao,&nbsp;Yujin Zhao,&nbsp;Jiaying Zhu,&nbsp;Shijie Dai,&nbsp;Xiaofeng Yuan","doi":"10.1002/bmc.70122","DOIUrl":"https://doi.org/10.1002/bmc.70122","url":null,"abstract":"<div>\u0000 \u0000 <p>Natural deep eutectic solvents (NADESs) have garnered significant attention for their application in the extraction of natural products. This study proposes a strategy to enhance the extraction efficiency of target compounds from the <i>Atractylodes macrocephala</i> Koidz–<i>Astragali Radix</i> (AM-AR) combination, and the crude extracts were employed for subsequent in vitro and in vivo investigations. The extraction efficiencies of nine NADESs and conventional solvents were compared for isolating calycosin 7-O-glucoside, ononin, quercetin, and atractylenolide III using high-performance liquid chromatography (HPLC) analysis. The extraction parameters were then optimized through response surface methodology (RSM) and genetic algorithm–back propagation neural network (GA-BPNN) models. In addition, the antioxidant activities were evaluated in vitro using assays such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) free radical scavenging, and ferric reducing antioxidant power (FRAP). An in vivo rat model of middle cerebral artery occlusion (MCAO) was utilized to assess the biological activities. Among the tested solvents, NADES-7 exhibited the highest extraction efficiency, achieving a comprehensive evaluation value of 0.173 and was selected for subsequent experiments. These findings indicate that NADESs can serve as promising alternative solvents for extracting natural products from traditional Chinese medicine (TCM).</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144100940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}