Biomedical Chromatography最新文献_第4页

LGZGD for Gestational Diabetes Mellitus: Integration of Traditional Chinese Medicine With Modern Research Evidence—A Letter to the Editor

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-07 DOI: 10.1002/bmc.70015

Lien-Chung Wei, Hsien-Jane Chiu

引用次数: 0

Bioanalytical Tandem Mass Spectrometry Method for Precise Measurement of Tranexamic Acid in Human Plasma With Unveiling Methodological Advancement: Green Assessment With Advanced Metrics

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-07 DOI: 10.1002/bmc.6086

Anil Kollapareddy, Kousrali Sayyad, Leela Prasad Kowtharapu, Naresh Konduru, Tanmoy Mondal, Mohan Varkolu, Sreedhar Gundekari

{"title":"Bioanalytical Tandem Mass Spectrometry Method for Precise Measurement of Tranexamic Acid in Human Plasma With Unveiling Methodological Advancement: Green Assessment With Advanced Metrics","authors":"Anil Kollapareddy, Kousrali Sayyad, Leela Prasad Kowtharapu, Naresh Konduru, Tanmoy Mondal, Mohan Varkolu, Sreedhar Gundekari","doi":"10.1002/bmc.6086","DOIUrl":"https://doi.org/10.1002/bmc.6086","url":null,"abstract":"<div>\u0000 \u0000 <p>For the control of excessive blood loss occurring from major trauma, postpartum bleeding, surgery, or hereditary angioedema, antifibrinolytic compound tranexamic acid (TXM) is highly efficient in controlling blood loss by reversibly binding with on lysine receptor sites. A selective and sensitive method has been developed and validated for the quantitation of TXM in human plasma using high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). Tranexamic acid-D2 (TXM-D2) was used as internal standard (ISTD) to minimize the errors in TXM quantification. TXM quantification was performed with positive polarity mode using a Shimadzu high performance liquid chromatography coupled with AB-SCIEX API-4000 tandem mass spectrometer. A Zorbax Eclipse C18 (150 × 4.6 mm, 5 μ) column was used for the TXM quantification, 8-mM ammonium formate buffer with 0.1% formic acid ionization enhancer was used as a mobile phase-A, and acetonitrile was used as a mobile phase-B. (38:62) v/v portion of mobile phases A and B selected to elute the TXM and TXM-D2 with the flow rate of 0.8 mL/min. An electrospray ionization (ESI) technique was selected for detection of TXM in the human plasma. Linearity was assessed in the concentration range from 75 to 15,000 ng/mL by using least squares of weighting factor linear regression (1/X<sup>2</sup>).</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Validated Liquid Chromatography–Tandem Mass Spectrometry Assay for the Determination of NX-5948 in Beagle Dog Plasma: Application to a Pharmacokinetic Study

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-07 DOI: 10.1002/bmc.6090

Fang Wang, Wenfei Sun, Zheheng Ma, Furui Chu

{"title":"A Validated Liquid Chromatography–Tandem Mass Spectrometry Assay for the Determination of NX-5948 in Beagle Dog Plasma: Application to a Pharmacokinetic Study","authors":"Fang Wang, Wenfei Sun, Zheheng Ma, Furui Chu","doi":"10.1002/bmc.6090","DOIUrl":"https://doi.org/10.1002/bmc.6090","url":null,"abstract":"<div>\u0000 \u0000 <p>Proteolysis targeting chimera (PROTAC) has been developed and currently enjoys widespread interest among the field of pharmaceutical manufacturing in recent decades. NX-5948 is an orally active PROTAC Bruton tyrosine kinase degrader, which shows anti-inflammatory and antitumor activities. In this study, a simple and fast bioanalytical method for the quantification of NX-5948 in beagle dog plasma was developed using liquid chromatography–tandem mass spectrometry (LC-MS/MS). A full method validation was performed according to regulatory guidelines. For the quantification, [M + H]<sup>+</sup> was formed using an electrospray ionization (ESI) source in the positive ion mode, and selective reaction monitoring (SRM) was employed using a triple quadrupole mass spectrometry. The monitored precursor-to-product transitions were <i>m/z</i> 807.5 > 790.5 for NX-5948 and <i>m/z</i> 812.4 > 452.1 for internal standard. A linear response was obtained at the concentration range of 0.5–200 ng/mL, with correlation coefficient > 0.99. The interday accuracy ranged from −9.03% to 5.99% (RSD < 7.84%), and the intraday accuracy from −5.15% to 8.56% (RSD < 6.90%). NX-5948 was demonstrated to be stable under the present storage conditions. After validation, the method was successfully applied for the quantification of NX-5948 in beagle dog plasma after oral and intravenous administration of the NX-5948.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unique Liquid Chromatography Technique for Determining a New Selective PPARα Modulator Drug (Pemafibrate) in the Tablet Dosage Form, Robustness by Design Expert in the Light of Quality by Design

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-07 DOI: 10.1002/bmc.70006

Fei Han, Yankun Yang, Zhonghu Bai, Rathnakar Nathi, Naresh Konduru

{"title":"Unique Liquid Chromatography Technique for Determining a New Selective PPARα Modulator Drug (Pemafibrate) in the Tablet Dosage Form, Robustness by Design Expert in the Light of Quality by Design","authors":"Fei Han, Yankun Yang, Zhonghu Bai, Rathnakar Nathi, Naresh Konduru","doi":"10.1002/bmc.70006","DOIUrl":"https://doi.org/10.1002/bmc.70006","url":null,"abstract":"<div>\u0000 \u0000 <p>This research study presents a precise, accurate, and linear liquid-chromatography method for the determination of pemafibrate in tablet dosage forms, despite the presence of a high volume of excipients. Various challenges were addressed through experimental approaches and evidence-based solutions. The method employs a suitable stationary phase, X-Bridge C<sub>18</sub> (150 × 4.6 mm, 3.5 μm), and an appropriate isocratic program. Key parameters include a flow rate of 1.0 mL/min, a column temperature of 40°C, an injection volume of 10 μL, and a runtime of 15 min. The wavelength was set at 210 nm because of its high response. Mobile phase optimization, based on experimental results, consists of 0.1% H<sub>3</sub>PO<sub>4</sub> buffer and acetonitrile in a 40:60 v/v ratio. The method has been validated according to ICH Q2 (R2) and Ch. P <9101> guidelines, achieving a recovery rate of 99.1% to 100.5% at levels of 50%, 100%, and 150%. Linearity was demonstrated from 25% to 300% concentration levels, with a correlation coefficient (<i>r</i><sup>2</sup>) value of 1.000. Precision results showed %RSD values of 1.0 and 1.1. Forced degradation studies indicated sensitivity to acid hydrolysis stress conditions and stability under physical stress conditions.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Chemical Constituents and Rat Metabolites of Jianpihuazhuotiaozhi Granule by Ultra-High-Performance Liquid Chromatography Coupled With Quadrupole-Time-of-Flight Mass Spectrometry

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-07 DOI: 10.1002/bmc.6080

Guo-Dong Hao, Zhi-Qiang Li, Yu-Wei Wang, Yuan-Ying Fang, Zhi-Feng Li, Qi Wang

{"title":"Identification of Chemical Constituents and Rat Metabolites of Jianpihuazhuotiaozhi Granule by Ultra-High-Performance Liquid Chromatography Coupled With Quadrupole-Time-of-Flight Mass Spectrometry","authors":"Guo-Dong Hao, Zhi-Qiang Li, Yu-Wei Wang, Yuan-Ying Fang, Zhi-Feng Li, Qi Wang","doi":"10.1002/bmc.6080","DOIUrl":"https://doi.org/10.1002/bmc.6080","url":null,"abstract":"<div>\u0000 \u0000 <p>Jianpihuazhuotiaozhi granules (JPHZTZ) are traditional Chinese medicine formula. An analytical method using ultra-high-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry was established to characterize the chemical constituents of JPHZTZ and determine its metabolic profile in rat urine and plasma after oral administration. A total of 220 compounds were identified in JPHZTZ extract, including 61 flavonoids, 16 alkaloids, 53 organic acids, 31 terpenoids, and 59 other compounds. Among these compounds, 11 were tentatively identified by comparing the retention times and mass spectral data with the corresponding reference standards and the literature; the other 209 components were tentatively identified based on their mass spectra alone. After the oral administration of JPHZTZ extract to rats, 13 prototypes and 54 metabolites were identified or tentatively characterized based on their retention time and mass spectra. The primary in vivo metabolic reactions that occurred after the administration of JPHZTZ extract included glucuronidation, sulfation, hydroxylation, and methylation. The 13 prototypes and 54 metabolites were identified in rat urine and plasma and were confirmed to be the potential active ingredients of JPHZTZ. Our findings provide a starting point for the further elucidation of the mechanism of action of JPHZTZ.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomic Analysis of Serum Reveals a Unique Metabolomic Profile in Patients With Myositis and Identified Several Potential Biomarkers in Polymyositis Versus Dermatomyositis Patients

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-02-07 DOI: 10.1002/bmc.70011

Shuoshan Xie, Caiyan Li, Congyu Lu, Yanjuan Liu

{"title":"Metabolomic Analysis of Serum Reveals a Unique Metabolomic Profile in Patients With Myositis and Identified Several Potential Biomarkers in Polymyositis Versus Dermatomyositis Patients","authors":"Shuoshan Xie, Caiyan Li, Congyu Lu, Yanjuan Liu","doi":"10.1002/bmc.70011","DOIUrl":"https://doi.org/10.1002/bmc.70011","url":null,"abstract":"<div>\u0000 \u0000 <p>DM and PM were the two major subtypes in myositis; among which, a unique metabolomic and biomarker profile remains lacking. Serum from 36 diagnosed myositis patients (28 DM and 8 PM) and 29 healthy controls was analyzed using HPLC-Q-TOF-MS/MS. PLS-DA was conducted through MetaboAnalyst 5.0 to identify the differential metabolites. The KEGG analysis was utilized to observe the related metabolic pathways. The potential biomarker value was assessed using ROC analysis. The relationship between the clinical characteristics and the levels of identified differential metabolites was analyzed using R language. PLS-DA showed a clear separation between healthy controls and myositis patients, and 131 differential metabolites were identified. KEGG analysis uncovered multiple disturbed metabolic pathways. Besides, nine differential metabolites were identified between PM and DM patients, which were involved in pentose and glucuronate interconversions. ROC curve analysis revealed the AUC of these identified metabolites is above 0.7. Among them, indoxyl sulfate, oleamide, and palmitoylethanolamide presented moderate or strong correlation with clinical characteristics. Metabolomics presents a different spectrum between myositis patients and healthy controls, PM and DM patients. Besides, indoxyl sulfate, oleamide, and palmitoylethanolamide may be potential biomarkers in distinguishing PM from DM.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Highly Sensitive Triple Quad LC–MS/MS Method Development and Validation for the Determination of Bexicaserin (LP352) in Human Plasma and Urine Matrices 高灵敏度三重四联质谱/质谱法测定人血浆和尿液基质中贝西卡塞林（LP352）的建立与验证

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-01-17 DOI: 10.1002/bmc.6079

Raja Reddy Kallem, Maisy Yeager, Rosa Chan, Katharine Fletcher, Katie Neal, Nuggehally R. Srinivas

{"title":"A Highly Sensitive Triple Quad LC–MS/MS Method Development and Validation for the Determination of Bexicaserin (LP352) in Human Plasma and Urine Matrices","authors":"Raja Reddy Kallem, Maisy Yeager, Rosa Chan, Katharine Fletcher, Katie Neal, Nuggehally R. Srinivas","doi":"10.1002/bmc.6079","DOIUrl":"10.1002/bmc.6079","url":null,"abstract":"<p>Bexicaserin is a highly selective agonist at the 5-HT<sub>2c</sub> receptor in clinical development for the treatment of seizures associated with developmental and epileptic encephalopathies (DEEs). We report an LC–MS/MS method for the quantitative estimation of bexicaserin in human plasma and urine. The sample preparation involves the extraction of bexicaserin and internal standard (<sup>13</sup>CD<sub>2</sub>-bexicaserin; IS) from 150 μL plasma and 50 μL urine using a solid phase extraction method. The chromatographic separation of bexicaserin and IS was achieved on a Poroshell EC-C18 column using 5 min gradient program. The calibration curve ranged from 0.1 to 100 ng/mL in plasma and 1.0 to 1000 ng/mL in urine. Intraday and interday precision and accuracy, linearity, matrix effect, extraction recovery, carry-over, dilution integrity, a battery of stability studies, and incurred sample reanalysis were performed for bexicaserin in both plasma and urine. The intraday and interday accuracy and precision were well within the acceptable limits in both plasma and urine matrices. Stability studies in plasma and urine showed that bexicaserin was stable on bench for 24 h, in autosampler over 54 h, five freeze–thaw cycles, and long-term storage at −20°C and −80°C for > 368 days. The validated methods were successfully applied in clinical study.</p>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11753905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening and Verification of Blood-Activating Effective Component Group of Panax notoginseng Based on Spectrum–Effect Relationships 基于谱效关系的三七活血有效成分群筛选与验证。

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-01-17 DOI: 10.1002/bmc.6083

Yuqing Wang, Mengmeng Liu, Yuxin Cao, Zhuangzhuang Hao, Jinfeng Liu, Yan Lyu, Jiayuan Li, Yu Wang, Tingyve Jiang, Wenxin Fan, Yifan Lu, Ge Zhang, Chunguo Wang, Jinli Shi

{"title":"Screening and Verification of Blood-Activating Effective Component Group of Panax notoginseng Based on Spectrum–Effect Relationships","authors":"Yuqing Wang, Mengmeng Liu, Yuxin Cao, Zhuangzhuang Hao, Jinfeng Liu, Yan Lyu, Jiayuan Li, Yu Wang, Tingyve Jiang, Wenxin Fan, Yifan Lu, Ge Zhang, Chunguo Wang, Jinli Shi","doi":"10.1002/bmc.6083","DOIUrl":"10.1002/bmc.6083","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Panax notoginseng</i> (<i>P. notoginseng</i>) is one of the most famous natural medicines and widely used to promote blood circulation in health care. However, the active component group of <i>P. notoginseng</i> for activating blood is not clear. We aim to screen and validate the pharmacodynamic component group (PCG), which could exert the same blood-activating effect as <i>P. notoginseng</i>. To clarify the active components, the chemical components were determined by liquid chromatography-tandem mass spectrometry, and the fingerprint of <i>P. notoginseng</i> was established. Twenty candidate active monomers were selected through the spectrum–effect relationship analysis. Eleven active monomers, including Ginsenoside Rg1, Rb1, Rd, F1, Rh1, Rg2, Rb2, Rg3, and Rk1 and Notoginsenoside R1 and R2, were screened out as the PCG through validation by platelet aggregation test. Among them, the antiplatelet aggregation activity of Ginsenoside Rh1 was directly confirmed for the first time. The active component group could exert similar efficacy to the <i>P. notoginseng</i> extract in vitro and in vivo through the validation of in vitro platelet aggregation test and the rats with cerebral ischemia. This study laid the foundation for the quality evaluation of <i>P. notoginseng</i> and provided a reference for the research on the material basis of the pharmacodynamics of other Chinese herbs.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142999483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multimodal Metabolomics Analysis Reveals That Classic Decoction Mitigates Myocardial Ischemia-Induced Damage by Modulating Energy and Branched-Chain Amino Acid Metabolism 多模式代谢组学分析揭示经典汤通过调节能量和支链氨基酸代谢减轻心肌缺血损伤。

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-01-11 DOI: 10.1002/bmc.6081

Zhimin Ying, Junling Pu, Yingying Hu, Junxiong Qian, Shengdong Luo, Feizheng Ni, Yi Tao

{"title":"Multimodal Metabolomics Analysis Reveals That Classic Decoction Mitigates Myocardial Ischemia-Induced Damage by Modulating Energy and Branched-Chain Amino Acid Metabolism","authors":"Zhimin Ying, Junling Pu, Yingying Hu, Junxiong Qian, Shengdong Luo, Feizheng Ni, Yi Tao","doi":"10.1002/bmc.6081","DOIUrl":"10.1002/bmc.6081","url":null,"abstract":"<div>\u0000 \u0000 <p>Gualou-Xiebai-Banxia (GXB) decoction shows potential for treating myocardial ischemia (MI), although its underlying mechanism is not fully understood. In this study, a multimodal metabolomics approach, combining gas chromatography–mass spectrometry (GC–MS) and <sup>1</sup>H-NMR, was employed to investigate the cardioprotective effects of GXB in a rat model of myocardial ischemia induced by ligation. ELISA assays and HE staining demonstrated that GXB effectively reduced myocardial injury, oxidative stress markers, and myocardial fibrosis. Orthogonal partial least-squares discriminant analysis identified 62 biomarkers, 20 of which were confirmed using standard compounds. The GC–MS method showed excellent linearity across a wide concentration range (0.004–29.7 μg/mL, <i>R</i><sup>2</sup> > 0.9995), with intra- and inter-day precision RSD values below 4.72% and 4.96%, respectively. Method recoveries ranged from 95.40% to 104.83%, with RSD values under 4.84%. Pathway enrichment analysis revealed that GXB decoction alleviates myocardial ischemia-induced damage primarily by modulating energy metabolism and branched-chain amino acid metabolism. These findings provide valuable support for the clinical application of GXB decoction in treating myocardial ischemia.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142963284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Network Pharmacology and Metabolomics to Investigate the Effects and Possible Mechanisms of Ginsenoside Rg2 Glycine Ester Derivative Against Hypoxia 综合网络药理学和代谢组学研究人参皂苷Rg2甘氨酸酯衍生物抗缺氧的作用及其可能机制。

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-01-11 DOI: 10.1002/bmc.6074

Qinghai Dong, Fei Shi, Fang Lin, Yaqi Wang, Yang An, Hongliu Xie, Jihua Liu

{"title":"Integrated Network Pharmacology and Metabolomics to Investigate the Effects and Possible Mechanisms of Ginsenoside Rg2 Glycine Ester Derivative Against Hypoxia","authors":"Qinghai Dong, Fei Shi, Fang Lin, Yaqi Wang, Yang An, Hongliu Xie, Jihua Liu","doi":"10.1002/bmc.6074","DOIUrl":"10.1002/bmc.6074","url":null,"abstract":"<div>\u0000 \u0000 <p>Previous studies have suggested that ginsenoside Rg<sub>2</sub> glycine ester derivative (RG) exhibits therapeutic potential in mitigating hypoxia. This study aimed to elucidate the potential mechanism of RG in hypoxia injury through a combined approach of metabolomics and network pharmacology. Initially, a CoCl<sub>2</sub>-induced cell hypoxia model was established, and the therapeutic impact of RG on biochemical indices was evaluated. Subsequently, metabolomics analysis of cell samples was conducted to identify biomarkers, and network pharmacology was employed to identify potential targets of RG for hypoxia treatment. Finally, the key target and pathway were verified. The study revealed that RG could reverse CoCl<sub>2</sub>-induced abnormalities in biochemical indicators. Metabolomics analysis identified 13 biomarkers and seven metabolic pathways associated with RG treatment. Utilizing network pharmacology, five key targets and five metabolic pathways were identified, partially aligning with the metabolomics results. Molecular docking results demonstrated the effective binding of RG to the key targets. Enzyme linked immunosorbent assay verified that RG could exert antihypoxia effect by activated PI3K/Akt pathway. In conclusion, this integrated strategy, combining metabolomics with network pharmacology, sheds light on the protective mechanism of RG against hypoxia-induced cellular damage. The findings offer valuable insights for future research and potential applications of RG in the field.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142963738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0