Biomedical Chromatography最新文献_第4页

A Simplified and Robust LC–MS/MS Method for Simultaneous Quantification of Six Steroid Hormones Associated With Polyendocrine Metabolic Ovarian Syndrome 一种简化、稳健的LC-MS/MS同时定量与卵巢多内分泌代谢性综合征相关的6种类固醇激素的方法

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-09-08 DOI: 10.1002/bmc.70609

Mengxue Feng, Yan Song, Xueqing Qian

{"title":"A Simplified and Robust LC–MS/MS Method for Simultaneous Quantification of Six Steroid Hormones Associated With Polyendocrine Metabolic Ovarian Syndrome","authors":"Mengxue Feng, Yan Song, Xueqing Qian","doi":"10.1002/bmc.70609","DOIUrl":"10.1002/bmc.70609","url":null,"abstract":"<div>\u0000 \u0000 <p>Polyendocrine metabolic ovarian syndrome (PMOS), formerly known as polycystic ovary syndrome (PCOS), is characterized by dysregulation of multiple steroid hormones. Accurate quantification of these analytes is essential for laboratory evaluation; however, currently may involve complex sample-preparation workflows or provide insufficient analytical coverage, particularly for dehydroepiandrosterone sulfate (DHEAS). A liquid chromatography–tandem mass spectrometry (LC–MS/MS) assay was established for the simultaneous measurement of six steroid hormones associated with PMOS. Sample preparation combined liquid–liquid extraction with protein precipitation. Chromatographic separation was completed within 6 min. Validation followed international recommendations and included linearity, sensitivity, precision, accuracy, matrix effects, selectivity, and specificity. All analytes showed excellent linearity (<i>R</i> = 0.9959–0.9995), with intra- and inter-day CVs ≤ 5.6%. Spike recovery ranged from 85.2% to 114.9%. Accuracy was further supported by third-party QC materials and successful participation in an external quality assessment program. The assay provided an expanded linear range for DHEAS (20–20,000 ng/mL). This validated LC–MS/MS assay provides rapid, sensitive, and reproducible quantification of six PCOS-related steroid hormones and is suitable for routine laboratory implementation.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148905033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Determination of Three Residual Monomers in Polyvinyl Alcohol Eye Drops Using a Dual-Wavelength RP-HPLC Method and Evaluation of Its Greenness 双波长反相高效液相色谱法同时测定聚乙烯醇滴眼液中残留的三种单体及其绿度评价。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-09-08 DOI: 10.1002/bmc.70613

Yang Zeyan, Gao Wenfen, Li Zhiyuan, Ma Xiaobin, Li Yongfei

{"title":"Simultaneous Determination of Three Residual Monomers in Polyvinyl Alcohol Eye Drops Using a Dual-Wavelength RP-HPLC Method and Evaluation of Its Greenness","authors":"Yang Zeyan, Gao Wenfen, Li Zhiyuan, Ma Xiaobin, Li Yongfei","doi":"10.1002/bmc.70613","DOIUrl":"10.1002/bmc.70613","url":null,"abstract":"<div>\u0000 \u0000 <p>Polyvinyl alcohol eye drops contain high-molecular-weight polymeric raw materials and excipients whose synthesis, storage, and pharmaceutical manufacturing processes may introduce or generate residual monomers, thereby affecting the quality, safety, and stability of the formulation. Therefore, establishing a reliable analytical method for the simultaneous determination of multiple residual monomers is essential for improving the quality control of such ophthalmic formulations. In this study, a dual-wavelength reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the simultaneous quantitative determination of 2-pyrrolidone, <i>N</i>-vinyl-2-pyrrolidone (NVP), and vinyl acetate (VA) in polyvinyl alcohol eye drops. Chromatographic separation was achieved on a NanoChrom 300 C18 column using an isocratic mobile phase consisting of water, methanol, and acetonitrile (90:5:5, v/v/v), with detection at 205 and 235 nm. The greenness and environmental sustainability of the proposed method were comprehensively evaluated using three complementary assessment tools: the Analytical Eco-Scale, the Analytical Method Greenness Score (AMGS), and the Modified Green Analytical Procedure Index (MoGAPI). The results demonstrated that the method is simple and provides satisfactory sensitivity, accuracy, and precision, making it suitable for the determination and quality control of the target residual compounds in ophthalmic formulations containing povidone as an excipient.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using UPLC–MS/MS and LC–MS/MS to Investigate the Pharmacokinetics in SD Rat Plasma and Tissue Distribution in KM Mice of Daphnoretin Nanomicelles 采用UPLC-MS/MS和LC-MS/MS研究了丹参素纳米微束在SD大鼠血浆中的药动学和在KM小鼠体内的组织分布。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-09-07 DOI: 10.1002/bmc.70614

Zhi-min Lv, Ju Zhang, Guo Feng, Xiang-ling Qu, Ting-ting Liu, Xin-ping Wei, Li Zhang, Shi-qiong Qin, Yun-li Wei, Gang Liu, Ke-xin Ma, Jin-xin Hou, Wei Li, Yi Sui

{"title":"Using UPLC–MS/MS and LC–MS/MS to Investigate the Pharmacokinetics in SD Rat Plasma and Tissue Distribution in KM Mice of Daphnoretin Nanomicelles","authors":"Zhi-min Lv, Ju Zhang, Guo Feng, Xiang-ling Qu, Ting-ting Liu, Xin-ping Wei, Li Zhang, Shi-qiong Qin, Yun-li Wei, Gang Liu, Ke-xin Ma, Jin-xin Hou, Wei Li, Yi Sui","doi":"10.1002/bmc.70614","DOIUrl":"10.1002/bmc.70614","url":null,"abstract":"<div>\u0000 \u0000 <p>Daphnoretin (DAP) has various pharmacological activities, but its in vivo disposition after nanomicellar formulation remains unclear. This study compared the pharmacokinetics and tissue distribution of free DAP and two polymeric nanomicellar formulations, PP-DAP and GA-DAP, following intravenous administration. Plasma DAP concentrations in rats were determined by UPLC–MS/MS, and DAP concentrations in mouse tissues were determined by LC–MS/MS. Compared with free DAP, PP-DAP and GA-DAP showed higher systemic exposure, longer apparent elimination half-lives, and lower apparent clearance. The AUC<sub>0–∞</sub> values of DAP, PP-DAP, and GA-DAP were 5474.14, 11,211.04, and 15,019.86 h · ng/mL, respectively, and the corresponding <i>T</i><sub>1/2</sub> values were 6.84, 9.24, and 9.90 h. DAP was detected in the heart, liver, spleen, lung, and kidney, with the nanomicellar formulations showing altered tissue distribution and GA-DAP exhibiting relatively sustained hepatic distribution. These findings suggest that nanomicellar formulation alters the in vivo disposition of DAP. However, free and micelle-associated DAP were not separately quantified, precluding direct characterization of in vivo drug release, and the validation range of the tissue quantification method was limited. Further studies are warranted to evaluate the in vivo behavior and liver-directed delivery potential of GA-DAP.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148905103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetic and Tissue Distribution Analysis of Scutellarin and Iso-Scutellarin From Breviscapine Injection in Rats by a Validated UPLC-MS/MS Method 灯盏花素注射液中灯盏花素和异灯盏花素在大鼠体内的药动学及组织分布分析

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-09-04 DOI: 10.1002/bmc.70608

Min Wen, Lijuan Fan, Lipan Yang, Qi Zhang, Xiaobo Lv

{"title":"Pharmacokinetic and Tissue Distribution Analysis of Scutellarin and Iso-Scutellarin From Breviscapine Injection in Rats by a Validated UPLC-MS/MS Method","authors":"Min Wen, Lijuan Fan, Lipan Yang, Qi Zhang, Xiaobo Lv","doi":"10.1002/bmc.70608","DOIUrl":"10.1002/bmc.70608","url":null,"abstract":"<div>\u0000 \u0000 <p>Breviscapine injection (BI), a standardized botanical drug for cardiovascular and cerebrovascular diseases, lacks adequate characterization of its pharmacokinetics and tissue distribution. A UPLC-MS/MS method was developed and validated for simultaneous quantification of scutellarin and its metabolite iso-scutellarin in rat plasma and tissues after intravenous BI administration. Sample preparation used methanol protein precipitation, with detection via positive electrospray ionization MRM. Validation followed the Chinese Pharmacopoeia and bioanalytical PK guidance. The assay showed LLQs of 10.0 ng/mL (scutellarin) and 5.0 ng/mL (iso-scutellarin), good linearity (<i>r</i><sup>2</sup> ≥ 0.990), precision (RSD ≤ 11.1%), accuracy (91.7%–106.8%), and acceptable recovery and matrix effects. PK analysis revealed moderate-to-rapid systemic elimination of scutellarin. Both analytes distributed to tissues within 0.25 h postdose, with exposure ranking: bladder > small intestine > kidney > small intestine > kidney > stomach > liver > plasma > heart > skeletal muscle > pancreas > skin > fat > lung > ovary > testis > adrenal > heart > skeletal muscle > pancreas > thymus > spleen > brain. The method is suitable for BI studies. The limited brain penetration suggests formulation optimization to prolong plasma exposure may be worth exploring, and the relatively high hepatic distribution points to a potential need for monitoring liver and kidney function.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148890936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stable Isotope-Resolved Metabolomics in Elucidating Mitochondrial Metabolic Reprogramming and Therapeutic Targets. 稳定同位素分解代谢组学在阐明线粒体代谢重编程和治疗靶点中的应用。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-09-01 DOI: 10.1002/bmc.70560

Dan Yang, Xinyan Wu, Xiufang Luo, Yuanyin Teng, Xiyue Duan, Tingting Kang, Wenze Wu

引用次数: 0

Phytochemical Profiling and Pharmacological Evaluation of Dichloromethane Extract From Aerial Parts of Ferula ferulaeoides for Antithrombotic, Anti-Inflammatory, and Analgesic Activities 阿魏地上部分二氯甲烷提取物抗血栓、抗炎和镇痛活性的植物化学特征和药理学评价。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-09-01 DOI: 10.1002/bmc.70568

Lina Liu, Qinghai Dong, Qi Zheng, Jieyu Shi, Shusen Liu, Zhihui Wang, Min Wang, Jianyong Si, Jingxue Ye, Wenlan Li, Junchi Wang, Guibo Sun

{"title":"Phytochemical Profiling and Pharmacological Evaluation of Dichloromethane Extract From Aerial Parts of Ferula ferulaeoides for Antithrombotic, Anti-Inflammatory, and Analgesic Activities","authors":"Lina Liu, Qinghai Dong, Qi Zheng, Jieyu Shi, Shusen Liu, Zhihui Wang, Min Wang, Jianyong Si, Jingxue Ye, Wenlan Li, Junchi Wang, Guibo Sun","doi":"10.1002/bmc.70568","DOIUrl":"10.1002/bmc.70568","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aims to elucidate the chemical composition of the dichloromethane extract from the aerial parts of <i>Ferula ferulaeoides</i> (DEAFF) and to explore its biological activities. The composition of DEAFF was analyzed by UHPLC-Q-Orbitrap-MS/MS. Then, the carrageenan-induced tail thrombosis model, xylene-induced ear swelling model, and acetic acid-induced writhing model were established to evaluate the relevant activities of DEAFF. Network pharmacology research and molecular docking were conducted to elucidate the pathways and key targets underlying the multiple activities of DEAFF. A total of 30 chemical constituents were tentatively identified in DEAFF based on UHPLC-Q-Orbitrap-MS/MS analysis. Pharmacodynamic results showed that DEAFF significantly reduced the proportion of thrombus in the tail of mice, effectively alleviated ear swelling, improved the degree of ear tissue lesions, and significantly reduced the number of writhing movements in mice, indicating that it has multiple pharmacological effects such as antithrombosis, anti-inflammation, and analgesia. Network pharmacology studies have shown that multiple pathways such as the PI3K-Akt signaling pathway and the MAPK signaling pathway play a crucial role in the pharmacological effects of DEAFF. In this study, the chemical components of DEAFF were preliminarily identified, and its effects on thrombosis prevention, anti-inflammation, and analgesia were systematically investigated.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148872823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Anti-Inflammatory Active Components in Morinda officinalis via Spectroscopic Effect Relationship Analysis and NF-κB Pathway Inhibition 通过光谱效应关系分析及NF-κB通路抑制鉴定巴戟天抗炎活性成分。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-08-31 DOI: 10.1002/bmc.70611

Kexu Dong, Yuan Zhang, Zihang Peng, Jing Lian, Qiushi Hu, Yuexin Zhu, Pengpeng Liu, Guoshun Shan, Yixiang Miao, Yurou Feng, Fan Zhang, Ji Shi

{"title":"Identification of Anti-Inflammatory Active Components in Morinda officinalis via Spectroscopic Effect Relationship Analysis and NF-κB Pathway Inhibition","authors":"Kexu Dong, Yuan Zhang, Zihang Peng, Jing Lian, Qiushi Hu, Yuexin Zhu, Pengpeng Liu, Guoshun Shan, Yixiang Miao, Yurou Feng, Fan Zhang, Ji Shi","doi":"10.1002/bmc.70611","DOIUrl":"10.1002/bmc.70611","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Morindae Officinalis</i> Radix (MOR), the dried root of <i>Morinda officinalis</i> How, contains abundant saccharides and is used clinically after different processing procedures. However, the anti-inflammatory material basis of MOR and the chemical consequences of <i>Lycium barbarum</i> processing remain insufficiently defined. In this study, high-performance liquid chromatography coupled with evaporative light scattering detection (HPLC-ELSD) was developed for fingerprint analysis of 15 batches of raw MOR and the corresponding <i>L. barbarum</i>-processed MOR (LB-MOR). Seventeen common peaks were resolved, and 11 saccharides were assigned by comparison with reference standards. Anti-inflammatory activity was evaluated in LPS-stimulated RAW 264.7 macrophages by quantifying nitric oxide (NO), TNF-α, IL-1β, and IL-6. Grey relational analysis and partial least squares regression linked the HPLC fingerprints with bioactivity and identified glucose, sucrose, kestose, and nystose as the principal activity-associated saccharides. Subsequent validation showed that these saccharides inhibited inflammatory mediator release, with kestose and nystose showing the strongest effects. Western blotting further indicated that kestose and nystose restored IκBα expression and decreased COX-2 expression and the p-p65/p65 ratio, consistent with suppression of sustained NF-κB signaling. This separation-based fingerprint and spectrum-effect workflow identify anti-inflammatory oligosaccharides in MOR and provides candidate quality markers for raw and processed MOR products.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148863465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and Preliminary Application of a Method to Detect Sucralose in Placenta and Serum 胎盘及血清中三氯蔗糖检测方法的建立及初步应用。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-08-31 DOI: 10.1002/bmc.70610

Ying He, Xin Yin, Run Ling, Yan Zhang, Yidan Ma, Junlin He

{"title":"Development and Preliminary Application of a Method to Detect Sucralose in Placenta and Serum","authors":"Ying He, Xin Yin, Run Ling, Yan Zhang, Yidan Ma, Junlin He","doi":"10.1002/bmc.70610","DOIUrl":"10.1002/bmc.70610","url":null,"abstract":"<div>\u0000 \u0000 <p>Sucralose, an artificial sweetener widely used in sugar-free foods and beverages, raises human exposure and potential accumulation in tissues and organs. Given the profound long-term impact of maternal diet on offspring, its safety during pregnancy is of particular concern. Existing detection methods primarily target food matrices and lack reliable, efficient approaches for biological sample analysis. To address this gap, we developed an ultrahigh-performance liquid chromatography–multiple reaction monitoring tandem mass spectrometry (UHPLC–MS/MS) method based on existing food detection methods to quantify sucralose in mouse serum and placenta. Method validation showed excellent linearity in the range of 20–1250 nmol/L, with limit of detection (LOD) of 4.88 nmol/L and limit of quantification (LOQ) of 9.77 nmol/L. The precision, accuracy, matrix effects, and freeze–thaw stability were all within acceptable limits. Using the method described above, we detected sucralose in both serum and placenta of mice following subchronic exposure, confirming its bioaccumulation, systemic absorption, and transplacental transfer, suggesting the need for further reproductive toxicity studies. In summary, this study developed and preliminarily applied a method for determining sucralose in mouse serum and placenta, providing a methodological reference for the toxicological evaluation and safety assessment of sucralose, and aids in improving food safety risk systems and the formulation of evidence-based public health policies.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148863554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of the Extraction of Total Alkaloids From Lotus Leaves by Acidic Ethanol and Its Purification by Macroporous Resins 酸性乙醇提取荷叶总生物碱的工艺优化及大孔树脂纯化。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-08-25 DOI: 10.1002/bmc.70601

Xueping Zhang, Yuchen Wu, Chenglin Li, Yichao Feng, Yichen Ge, Baoyang Deng, Siying Yang, Yaosen Yang, Jianqing Su, Xiuling Chu

{"title":"Optimization of the Extraction of Total Alkaloids From Lotus Leaves by Acidic Ethanol and Its Purification by Macroporous Resins","authors":"Xueping Zhang, Yuchen Wu, Chenglin Li, Yichao Feng, Yichen Ge, Baoyang Deng, Siying Yang, Yaosen Yang, Jianqing Su, Xiuling Chu","doi":"10.1002/bmc.70601","DOIUrl":"10.1002/bmc.70601","url":null,"abstract":"<div>\u0000 \u0000 <p>This acidic ethanol extraction method was adopted to extract total alkaloids from <i>lotus</i> leaves. Single-factor experiments combined with response surface methodology (RSM) were employed to optimite extraction conditions. The optimal extraction parameters were determined as follows: 55 °C, 77% ethanol, 0.66% hydrochloric acid, under which the extraction yield of total alkaloids reached 4.14%. D101 macroporous resin was selected for the purification of crude alkaloid extracts. The resin exhibited an adsorption capacity of 8.60 mg/g and a desorption rate of 43.39%. The optimized purification conditions were as follows: 4 mg/mL sample solution, 50% ethanol eluent, 1.5 BV/h flow rate, 30 mL loading/100 mL elution volume. After purification, total alkaloid purity reached 89.31 ± 1.33%. Adsorption obeyed pseudo-second-order kinetics and Temkin isotherm, being spontaneous and endothermic. This provides improved methods for isolating bioactive alkaloids from <i>lotus</i> leaf.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148811946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum Pharmacochemistry Combined With Network Pharmacology to Explore Potential Bioactive Components and Mechanisms of Pomegranate Peel in the Treatment of Colorectal Cancer 血清药物化学联合网络药理学探讨石榴皮治疗大肠癌的潜在生物活性成分及其作用机制。

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2026-08-25 DOI: 10.1002/bmc.70605

Zainaipuguli Yisimayili, Guzilinuer Yimamu, Reziya Aimaiti, Maihesumu Aikemu, Kurexi Yunusi

{"title":"Serum Pharmacochemistry Combined With Network Pharmacology to Explore Potential Bioactive Components and Mechanisms of Pomegranate Peel in the Treatment of Colorectal Cancer","authors":"Zainaipuguli Yisimayili, Guzilinuer Yimamu, Reziya Aimaiti, Maihesumu Aikemu, Kurexi Yunusi","doi":"10.1002/bmc.70605","DOIUrl":"10.1002/bmc.70605","url":null,"abstract":"<div>\u0000 \u0000 <p>Pomegranate peel (PP) is a traditional Chinese medicinal material. PP has multiple biological activities such as anti-inflammatory and anticancer activities. Although biological activity investigations of PP are growing, few studies have focused on its chemical constituents, and there is a lack of research on its in vivo metabolic characteristics. In this study, UHPLC-Orbitrap Exploris-MS was used to analyze constituents of PP and, for the first time, its metabolic profiles. Network pharmacology and molecular docking were used to elucidate anti-colorectal cancer mechanisms of PP. As a result, we identified 131 compounds in PP. Among them, multiple isomeric forms of different types of ellagitannins and gallotannins were simultaneously identified for the first time. Furthermore, we identified 14 absorbed components and their eight metabolites in rat serum after oral administration of PP. In network pharmacology and molecular docking analysis, using 14 absorbed compounds, we obtained six core targets and five main absorbed compounds that were considered possible anti-colorectal cancer targets and bioactive compounds of PP. Our findings provide systematic knowledge on the material basis, metabolic profiles, and anti-colorectal cancer mechanisms of PP. This study also provides further understanding of ellagitannins and gallotannins in medicinal plants/foods, as well as their in vivo metabolic characteristics.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 10","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148817374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0