Biomedical Chromatography最新文献_第5页

A New Discovery: Corydalis yanhusuo Causes Idiosyncratic Hepatotoxicity and Its Potential Mechanisms 延胡索引起特异性肝毒性及其潜在机制的新发现

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-04-25 DOI: 10.1002/bmc.70093

Longxin Guo, Li Lin, Jun Ling, Shengkai Zhu, Xinyu Li, Minjuan Long, Yingjie Xu, Zhanjiang Hu, Ming Niu, Xu Zhao, Xiaohe Xiao

{"title":"A New Discovery: Corydalis yanhusuo Causes Idiosyncratic Hepatotoxicity and Its Potential Mechanisms","authors":"Longxin Guo, Li Lin, Jun Ling, Shengkai Zhu, Xinyu Li, Minjuan Long, Yingjie Xu, Zhanjiang Hu, Ming Niu, Xu Zhao, Xiaohe Xiao","doi":"10.1002/bmc.70093","DOIUrl":"https://doi.org/10.1002/bmc.70093","url":null,"abstract":"<div>\u0000 \u0000 Corydalis yanhusuo W.T.Wang (YHS) is a commonly used traditional Chinese medicine, often prescribed for treating a variety of pains. In recent years, there has been a gradual increase in the number of reports to liver injury caused by YHS and its preparations, but the exact type and mechanism of hepatotoxicity are still unclear. In the present study, we demonstrated that YHS could induce idiosyncratic drug-induced liver injury (IDILI) in the inflammatory activation models. A total of 459 differential genes and 25 differential metabolites were identified by transcriptomics and metabolomics, which were significantly enriched in the TNF and NF-κB signaling pathways as well as glycerophospholipid metabolism, sphingolipid metabolism, and arachidonic acid metabolism. In addition, YHS significantly increased the levels of TNF-α, IL-1β, and IL-6. Therefore, we believe that the mechanism of toxicity may be related to the TNF and NF-κB signaling pathways, with glycerophospholipid metabolism, sphingolipid metabolism, and arachidonic acid metabolism also playing important roles. It provides a reference for the safe and rational use of YHS in clinical practice and contributes to the precise prevention and control of the risk of liver toxicity associated with YHS.\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143871742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Mechanisms of Polygonum orientale L. Against Myocardial Ischemia: An Integrated Analysis Using Ultra-High-Performance Liquid Chromatography–Quadrupole Exactive Orbitrap Mass Spectrometry, Network Pharmacology, and RNA Sequencing 探索何首乌防治心肌缺血的机制：利用超高效液相色谱-四极杆精确轨道阱质谱、网络药理学和 RNA 测序进行综合分析

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-04-21 DOI: 10.1002/bmc.70089

Chunhua Liu, Changli Fu, Luping Tang, Jieqi Li, Jia Sun, Yuan Lu, Jie Pan, Ting Liu, Yongjun Li, Yonglin Wang, Yong Huang, Yueting Li, Meng Zhou

{"title":"Exploring the Mechanisms of Polygonum orientale L. Against Myocardial Ischemia: An Integrated Analysis Using Ultra-High-Performance Liquid Chromatography–Quadrupole Exactive Orbitrap Mass Spectrometry, Network Pharmacology, and RNA Sequencing","authors":"Chunhua Liu, Changli Fu, Luping Tang, Jieqi Li, Jia Sun, Yuan Lu, Jie Pan, Ting Liu, Yongjun Li, Yonglin Wang, Yong Huang, Yueting Li, Meng Zhou","doi":"10.1002/bmc.70089","DOIUrl":"https://doi.org/10.1002/bmc.70089","url":null,"abstract":"<div>\u0000 \u0000 Polygonum orientale L. (PO) represents significant bioactivities in treating myocardial ischemia (MI); however, its underlying mechanisms remain unclear. This study aims to elucidate PO's potential mechanisms in MI using an integrated approach that combines UHPLC–Q-Exactive Orbitrap HRMS, network pharmacology, and RNA-sequencing (RNA-seq). Initially, the chemical constituents of PO were identified using UHPLC–Q-Exactive Orbitrap HRMS. Subsequently, network pharmacology, molecular docking, and RNA-seq were employed to screen potential active components of PO targeting MI and predict their molecular mechanisms. Then, the molecular mechanisms were verified using western blotting and ELISA in MI mice. A total of 45 components were identified from PO, with 14 potential active compounds interacting with 204 MI-related genes. The findings suggest that PO could alleviate heart damage. The RNA-seq results indicated 244 potential targets regulated by PO. Integrating RNA-seq and network pharmacology analyses revealed that the toll-like receptor signaling pathway plays an important role, alongside the PI3K-Akt. Notably, PO reduced the expression of TLR4 and TLR2 while increasing p-Akt and p-PI3K levels in MI mice, leading to decreased inflammatory cytokines and apoptosis-related proteins. This study provides initial evidence that PO inhibits the toll-like signaling pathway and activates PI3K–Akt signaling pathway to exert protective effects against MI.\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetics of Oleracrylimide E in Rats Plasma Using UHPLC/-ESI-Q-TOF/MS 用UHPLC/-ESI-Q-TOF/MS研究奥丙烯酰亚胺E在大鼠血浆中的药动学

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-04-20 DOI: 10.1002/bmc.70067

Zheming Ying, Kaiyun Jiang, Chengyu Wang, Hongzhe Zhang, Junjie Yao, Yingdai Zhao, Xixiang Ying, Yanling Ren

引用次数: 0

Quantification of Methylene Blue and Evaluation of Its Pharmacokinetics in ICR Mice by Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry Using Difluoroacetic Acid 使用二氟乙酸的液相色谱-四极杆飞行时间质谱法定量亚甲基蓝并评估其在 ICR 小鼠体内的药代动力学

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-04-20 DOI: 10.1002/bmc.70080

Seo-jin Park, Juwon Lee, Sangsoo Hwang, Jeong-hyeon Lim, Hyunjin Cho, Young G. Shin

{"title":"Quantification of Methylene Blue and Evaluation of Its Pharmacokinetics in ICR Mice by Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry Using Difluoroacetic Acid","authors":"Seo-jin Park, Juwon Lee, Sangsoo Hwang, Jeong-hyeon Lim, Hyunjin Cho, Young G. Shin","doi":"10.1002/bmc.70080","DOIUrl":"https://doi.org/10.1002/bmc.70080","url":null,"abstract":"Methylene blue (MB), a phenothiazine derivative, is currently under clinical trials for Alzheimer's disease (AD) due to its potential to inhibit tau aggregation, a key pathological process in AD. In this study, we developed and qualified a rapid and reliable liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-qTOF-MS) method for the quantification of MB in mouse plasma and brain samples. Chromatographic separation was achieved using a PolymerX RP-1 100 Å (50 × 2 mm, 5 μm) column with a mobile phase consisting of water and methanol containing 0.5% difluoroacetic acid, delivered at a flow rate of 0.5 mL/min. Calibration curves were constructed using quadratic regression (weighted 1/concentration2) over a range of 3.05–2222.22 ng/mL in both matrices. The method was successfully applied to characterize the pharmacokinetics of MB in male ICR mice, revealing a high systemic clearance (65.64 mL/min/kg) and substantial brain penetration, as indicated by a brain-to-plasma partition coefficient (Kp,brain) of 23.50 following single intravenous bolus administration. These findings provide critical insights into MB's in vivo behavior and demonstrate the utility of this bioanalytical method for evaluating MB in preclinical studies.","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bmc.70080","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative Analysis of Bimiralisib in Dried Blood Spots of Mouse Blood Using a Validated LC-MS/MS Method: An Application to Pharmacokinetic Study hplc -MS/MS法定量分析小鼠血干血斑中比米拉利布的药代动力学研究

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-04-20 DOI: 10.1002/bmc.70087

Ashok Zakkula, M. Akiful Haque

{"title":"Quantitative Analysis of Bimiralisib in Dried Blood Spots of Mouse Blood Using a Validated LC-MS/MS Method: An Application to Pharmacokinetic Study","authors":"Ashok Zakkula, M. Akiful Haque","doi":"10.1002/bmc.70087","DOIUrl":"https://doi.org/10.1002/bmc.70087","url":null,"abstract":"<div>\u0000 \u0000 Bimiralisib, a pan-PI3K/mTOR inhibitor, has demonstrated antitumor efficacy in preclinical models. In this study, we present a validated LC-MS/MS method for quantifying bimiralisib from dried blood spots (DBS) in mice. The method was validated in accordance with FDA guidelines. Bimiralisib was extracted from DBS disks using a liquid–liquid extraction technique. Chromatographic separation was achieved on an Atlantis dC18 column (100 × 4.6 mm) using an isocratic mobile phase. The flow rate was set at 0.70 mL/min. Under optimized conditions, the retention times for bimiralisib and the internal standard (IS, Nilotinib) were approximately 1.14 and 1.27 min, respectively, with a total run time of 2.00 min per injection. The monitored MS/MS ion transitions were m/z 412.2 → 141.0 for bimiralisib and 530.4 → 259.0 for the IS. The method employed a broad calibration range (1.00–1434 ng/mL) with a determination coefficient (r2) of 0.996. All validation parameters met the required acceptance criteria, and hematocrit levels had no impact on bimiralisib concentrations in DBS. The validated method was utilized to determine intravenous and oral pharmacokinetic parameters by quantifying bimiralisib in mouse blood, with results correlated to pharmacokinetic data from mice plasma.\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 6","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetics and Tissue Distribution Study for 2-(2-Fluorophenyl)-5-Phenyl-7-Alkoxy- [1,2,4]Triazole[1,5-a]Pyrimidine Antiepileptic Compounds in Rats 2-（2-氟苯基）-5-苯基-7-烷氧基-[1,2,4]三唑[1,5-a]嘧啶类抗癫痫药在大鼠体内的药动学及组织分布研究

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-04-15 DOI: 10.1002/bmc.70088

Han Liang, Ranran Guo, Yuxi Zhou, Cui Lv, Chuanlong Guo, Feng Su, Qingkun Dong, Longjiang Huang, Wen Xu

{"title":"Pharmacokinetics and Tissue Distribution Study for 2-(2-Fluorophenyl)-5-Phenyl-7-Alkoxy- [1,2,4]Triazole[1,5-a]Pyrimidine Antiepileptic Compounds in Rats","authors":"Han Liang, Ranran Guo, Yuxi Zhou, Cui Lv, Chuanlong Guo, Feng Su, Qingkun Dong, Longjiang Huang, Wen Xu","doi":"10.1002/bmc.70088","DOIUrl":"https://doi.org/10.1002/bmc.70088","url":null,"abstract":"<div>\u0000 \u0000 The development of novel active molecules with a clear target has always been an urgent need for the treatment of epilepsy. Previously, we reported 2-(2-fluorophenyl)-5-phenyl-7-propyl-[1,2,4]triazolo[1,5-a]pyrimidine (10C) as a selective and positive modulator of GABAA receptors, which exhibited excellent antiepileptic activity in mice with an ED50 value of 8.51 mg/kg. However, the pharmacokinetics (PK) profiles of this compound remain unclear. In this study, 10C and four analogs (10A, 10B, 10D, and 10E) as well as deuterated 10C were synthesized with high efficiency under optimized reaction conditions, and deuterated 10C was employed as an internal standard. The concentrations of the five compounds in rat plasma and of 10C in tissue homogenate were assayed using a liquid chromatography–tandem mass spectrometry (LC-MS/MS) method. The results indicated that, compared to the other four analogs, 10C exhibited the highest drug concentration in rat plasma at the same dosage, which provides a good explanation for its superior antiepileptic activity compared to the other four analogs. Following intraperitoneal injection, 10C displayed favorable pharmacokinetic characteristics (F = 41%) and excellent brain penetration potency (B/P = 1.9). Overall, compound 10C is a promising lead for the research and development of new small-molecule therapeutics for epilepsy.\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143831413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the Effective Components and Molecular Mechanisms of Xuanbi Decoction for Treating Gouty Arthritis: An Integrated Approach Using Metabolomics and Network Pharmacology 玄痹汤治疗痛风性关节炎的有效成分及分子机制：代谢组学和网络药理学的综合研究

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-04-15 DOI: 10.1002/bmc.70086

Yundong Xu, Xiaoyu Zhang, Niqin Xiao, Qianqian Yang, Heguo Yan, Hongting Lu, Zhaohu Xie, Zhaofu Li

{"title":"Unraveling the Effective Components and Molecular Mechanisms of Xuanbi Decoction for Treating Gouty Arthritis: An Integrated Approach Using Metabolomics and Network Pharmacology","authors":"Yundong Xu, Xiaoyu Zhang, Niqin Xiao, Qianqian Yang, Heguo Yan, Hongting Lu, Zhaohu Xie, Zhaofu Li","doi":"10.1002/bmc.70086","DOIUrl":"https://doi.org/10.1002/bmc.70086","url":null,"abstract":"<div>\u0000 \u0000 Xuanbi Decoction (XBD) is a classical traditional Chinese medicine (TCM) effective in treating different types of arthritis. This study aimed to integrate metabolomics with network pharmacology to identify active metabolic components of XBD, elucidate its therapeutic targets, and reveal the key signaling pathways involved in the treatment of gout. The study systematically analyzed the material basis and potential mechanisms underlying XBD efficacy in gouty arthritis (GA). First, 352 blood metabolites from XBD were screened by extracting the drug-containing serum and utilizing liquid chromatography–tandem mass spectrometry (LC-MS/MS). Twenty-two key ones were identified through correlation analysis. Two-hundred fifty-five metabolite-related targets and 764 GA-related targets were retrieved from multiple databases. Further analysis of the intersection of targets identified 60 key overlapping targets. PPI network analysis elucidated the interrelationships among the 60 targets. GO and KEGG pathway enrichment analyses were conducted on these crossover targets, identifying 25 GO terms and 20 KEGG pathways. Network diagrams were constructed, featuring “22 metabolites–60 targets–25 GO terms” and “22 metabolites–60 targets–20 KEGG pathways.” Additionally, a comprehensive network map was constructed, featuring “9 XBD drugs–22 active metabolic components–60 core targets–25 signaling pathways,” elucidating the multidimensional intervention mechanism of XBD on GA, offering insights into its clinical application in GA treatment.\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143831414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination by UHPLC-QTOF-HRMS of Phosphatidylethanol (PEth) in Dried Blood Spots: Method Validation and Practical Application of a Rising Alcohol Abuse Biomarker With Minimally Invasive Sampling UHPLC-QTOF-HRMS法测定干血斑中磷脂酰乙醇（PEth）：方法验证及微创进样新酒精滥用生物标志物的实际应用

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-04-11 DOI: 10.1002/bmc.70081

Christina Ververi, Marta Massano, Eugenio Alladio, Alberto Salomone, Marco Vincenti

{"title":"Determination by UHPLC-QTOF-HRMS of Phosphatidylethanol (PEth) in Dried Blood Spots: Method Validation and Practical Application of a Rising Alcohol Abuse Biomarker With Minimally Invasive Sampling","authors":"Christina Ververi, Marta Massano, Eugenio Alladio, Alberto Salomone, Marco Vincenti","doi":"10.1002/bmc.70081","DOIUrl":"https://doi.org/10.1002/bmc.70081","url":null,"abstract":"The goal of our study was to develop and validate a simple, quick, and sensitive method to detect phosphatidylethanol (PEth) in dried blood spots (DBS). A 30-μL aliquot of blood was collected on a DBS card and allowed to dry at room temperature. Then, the spot was cut and transferred into a clean tube where the internal standard (PEth-D5) and 1-mL hexane were added followed by stirring, sonication, and centrifugation at room temperature. The dried supernatant was reconstituted with 30-μL acetonitrile and analyzed by UHPLC-HRMS-QTOF. Calibration curve was created at 20, 50, 100, 200, 300, and 500 ng/mL; the limit of detection was calculated at 5 ng/mL (S/N > 3) while accuracy, precision, recovery, and matrix effect were successfully evaluated, along with the analyte stability at different time intervals and temperatures. The study demonstrates that quantifying PEth 16:0/18:1 from DBS cards is feasible using UHPLC-QTOF or UHPLC-QqQ instrumentation while the QTOF method was validated and proved reliable for PEth detection to assess both excessive alcohol consumption and alcohol abstinence, matching current guidelines. Preliminary data on authentic samples confirmed the method's performance in terms of ease, sustainability, and speed, supporting its great potential for routine toxicological diagnosis of chronic alcohol abuse.","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bmc.70081","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143818328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomic Profiling of Three Body Fluids Differentiates UWS and MCS in Disorders of Consciousness 三种体液的代谢组学分析可区分意识障碍中的UWS和MCS

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-04-11 DOI: 10.1002/bmc.70079

Aiwei Wang, Long Xu, Fei Xue, Hezhen Lu, Xiaoyan Liu, Haidan Sun, Zhengguang Guo, Qianqian Ge, Xiaoli Geng, Xueling Chen, Binbin Zhang, Jiameng Sun, Feng Qi, Xia Niu, Ying Lan, Jianghong He, Wei Sun

{"title":"Metabolomic Profiling of Three Body Fluids Differentiates UWS and MCS in Disorders of Consciousness","authors":"Aiwei Wang, Long Xu, Fei Xue, Hezhen Lu, Xiaoyan Liu, Haidan Sun, Zhengguang Guo, Qianqian Ge, Xiaoli Geng, Xueling Chen, Binbin Zhang, Jiameng Sun, Feng Qi, Xia Niu, Ying Lan, Jianghong He, Wei Sun","doi":"10.1002/bmc.70079","DOIUrl":"https://doi.org/10.1002/bmc.70079","url":null,"abstract":"<div>\u0000 \u0000 Disorders of consciousness (DOC), including unresponsive wakefulness syndrome (UWS) and minimally conscious state (MCS), are complex brain dysfunctions with various causes. Misdiagnosis is common when relying solely on neurological exams, highlighting the need for accurate differentiation to guide clinical rehabilitation. This study explores metabolomic differences between UWS and MCS across cerebrospinal fluid (CSF), serum, and urine samples to identify biomarkers and metabolic pathways. Fifty-one subjects were categorized into UWS (n = 35) and MCS (n = 16) based on coma recovery scale-revised (CRS-R) scores. Ultraperformance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) was used to analyze samples, and statistical methods identified 14, 24, and 22 differential metabolites in CSF, serum, and urine, respectively. CSF metabolites were linked to necrosis, apoptosis, and neuroprotection; serum metabolites to lipid metabolism and immune response; and urine metabolites to cell signaling and neural function. Metabolomic panels showed AUC values of 0.85 (95% CI: 0.73–0.96) for CSF (95% CI: 0.86–1.00), 0.94 for serum, and 0.93 (95% CI: 0.79–1.00) for urine in distinguishing UWS from MCS. This profiling offers valuable insights into DOC pathophysiology and aids in accurate differentiation of these states.\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143818327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the Material Basis of Taxillus chinensis in the Treatment of Hyperuricemia Nephropathy Through Absorbed Into Blood Component Analysis and Network Pharmacology 通过血液成分分析及网络药理学探讨红豆杉治疗高尿酸血症肾病的物质基础

IF 1.8 4区医学

Biomedical Chromatography Pub Date : 2025-04-11 DOI: 10.1002/bmc.70066

Jiemei Liang, Hua Zhu, Qiyuan Yang, Zhouwei Li, Xiang Meng, Lanlan Fan, Li Li

{"title":"Exploring the Material Basis of Taxillus chinensis in the Treatment of Hyperuricemia Nephropathy Through Absorbed Into Blood Component Analysis and Network Pharmacology","authors":"Jiemei Liang, Hua Zhu, Qiyuan Yang, Zhouwei Li, Xiang Meng, Lanlan Fan, Li Li","doi":"10.1002/bmc.70066","DOIUrl":"https://doi.org/10.1002/bmc.70066","url":null,"abstract":"<div>\u0000 \u0000 Taxillus chinensis (DC) Danser (T. chinensis) is broadly used in traditional Chinese medicine. Although pharmacological research shows that the ethyl acetate extract of T. chinensis (JEA) has beneficial effects in treating hyperuricemic nephropathy (HN), the active components and potential mechanisms for these effects remain unclear. This study aims to predict the effective components and mechanism of JEA for HN. Firstly, we adopted UHPLC-Q-Exactive HFXMS technology to analyze the chemical profile of JEA and the absorbed prototype ingredients in rat plasma. In addition, network pharmacology methods were utilized by us to elaborate on the active compounds, signaling pathways, and potential mechanisms of JEA in treating HN. Finally, a UPLC method was established to screen potential chemicals that can effectively inhibit the activity of xanthine oxidase (XOD). A total of 92 components were systematically characterized in JEA. Of those, 46 compounds were identified in the plasma of rats administered with JEA extract. Through network pharmacology, 10 potential active components, 10 crucial target genes, and 20 pathways were predicted to be involved in the JEA-mediated treatment of HN. The molecular docking results indicated that oxyresveratrol, isorhammeiol, and robinetwere exhibited strong binding affinities for GAPDH, PPARG, and ALB. The analysis of the XOD inhibition experiment suggested that dihydromyricetin and oxyresveratrol exhibited potent inhibitory effects on XOD, with IC50 values of 0.48 and 0.68 mg·mL−1. This study preliminarily identified the potential effective components of JEA and revealed its underlying molecular mechanisms of action in preventing HN, which will improve our further studies on JEA to treat HN.\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 5","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143818330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0