Biomedical Chromatography最新文献_第6页

A Rapid and Simple Reversed-Phase HPLC–MS/MS Determination of Baloxavir in Rat Plasma and Its Application in Pharmacokinetic Study 快速简便反相HPLC-MS /MS法测定大鼠血浆中巴洛韦的含量及其在药动学研究中的应用

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-08-03 DOI: 10.1002/bmc.70183

Peng Guoshuang, Wan Lili, Liu Xiang, Li Bei, Sun Yongbing

引用次数: 0

Quantitation of Niraparib in Human Plasma by LC-MS/MS LC-MS/MS定量人血浆中尼拉帕尼的含量

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-07-31 DOI: 10.1002/bmc.70181

Tien V. Le, Sarah E. Taylor, Robert A. Parise, Susan M. Christner, Cortney Montgomery, Timothy Synold, Ye Feng, Jan H. Beumer

{"title":"Quantitation of Niraparib in Human Plasma by LC-MS/MS","authors":"Tien V. Le, Sarah E. Taylor, Robert A. Parise, Susan M. Christner, Cortney Montgomery, Timothy Synold, Ye Feng, Jan H. Beumer","doi":"10.1002/bmc.70181","DOIUrl":"10.1002/bmc.70181","url":null,"abstract":"<div>\u0000 \u0000 <p>Polyadenosine diphosphate–ribose polymerase 1 (PARP-1) and 2 (PARP-2) are key DNA repair enzymes that promote single-strand break repair via the base excision pathway. Niraparib, a PARP inhibitor, has shown clinical efficacy with the reduction of disease progression or death and progression-free survival benefit across multiple clinical trials, leading to the Food and Drug Administration (FDA) approval for the treatment of advanced and recurrent ovarian cancers. This study presents a robust and simple 5-min assay designed for the quantitation of the single agent niraparib in human plasma utilizing liquid chromatography–tandem mass spectrometry (LC-MS/MS). A 50 μL volume of plasma was subjected to protein precipitation, followed by chromatographic separation using a Phenomenex Kinetex C18 column (2.6 μm, 50 × 2.1 mm) and a gradient mobile phase system consisting of 0.1% formic acid in both water and acetonitrile during a 5-min run time. Mass spectrometric detection was achieved using a SCIEX 6500 + tandem mass spectrometer with electrospray positive-mode ionization. With a stable isotopic internal standard, our assay met the criteria outlined by the FDA guidance for bioanalytical method validation, demonstrating robust performance within the range from 5 to 5000 ng/mL. This assay will support future clinical studies by defining niraparib pharmacokinetics.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144751693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UPLC-Q/TOF-MS-Based Serum Metabolomics Reveals the Potential Antihyperglycemic Mechanisms of Polygonatum sibiricum in KK/Upj-Ay Type 2 Diabetic Mice 基于UPLC-Q/ tof - ms的血清代谢组学揭示了黄精对KK/Upj-Ay 2型糖尿病小鼠的潜在降糖机制

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-07-29 DOI: 10.1002/bmc.70177

Hao Li, Shuhan Tang, Jinyue Huang, Yidan Sun, Yaqi Xu, Yige Wang, XinTong Li, Minyue Zhang, Rong Ma, Xianna Li, Hongying Xu, Hailong Zhang, Zhigang Wang

{"title":"UPLC-Q/TOF-MS-Based Serum Metabolomics Reveals the Potential Antihyperglycemic Mechanisms of Polygonatum sibiricum in KK/Upj-Ay Type 2 Diabetic Mice","authors":"Hao Li, Shuhan Tang, Jinyue Huang, Yidan Sun, Yaqi Xu, Yige Wang, XinTong Li, Minyue Zhang, Rong Ma, Xianna Li, Hongying Xu, Hailong Zhang, Zhigang Wang","doi":"10.1002/bmc.70177","DOIUrl":"10.1002/bmc.70177","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Polygonatum sibiricum</i> (PS) is a traditional Chinese medicinal herb used as both food and medicinal material. PS is reportedly significantly effective in the treatment of type 2 diabetes mellitus (T2DM) in clinic. However, its pharmacological mechanism and endogenous antihyperglycemic markers remain unknown. This study investigated PS effects in KK/Upj-Ay 2 diabetic mice and revealed its potential antihyperglycemic mechanisms. Nontargeted serum metabolomics and pathophysiological detection characterized differentially abundant metabolites and determined changes in metabolic pathways. Fasting blood glucose decreased, while serum total cholesterol (TC), tumor necrosis factor-α (TNF-α), triglyceride (TG), and whole blood hemoglobin A1c (HbA1c) levels in kidney tissue homogenates were restored to different degrees following administration with PS. Observation of hematoxylin–eosin (HE) staining of the kidney and pancreas, periodic acid-Schiff (PAS) staining of the kidney, and immunohistochemistry of pancreatic insulin revealed that PS could reduce dysfunction in KK/Upj-A<sup>y</sup> 2 diabetic mice. Nontargeted serum metabolomics identified 37 T2DM biomarkers, 27 of which were significantly recovered by high-dose PS. Further pathway analysis revealed 11 key metabolic pathways involved in PS-mediated effects. These pathways regulate inflammation, lipid accumulation, oxidative stress, and insulin resistance, elucidating multitarget mechanisms by which PS ameliorates metabolic disturbances and exerts antihyperglycemic effects in KK/Upj-A<sup>y</sup> type 2 diabetic mice.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144717033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Dendrobium devonianum Samples With Different Growth Years Based on Active Ingredients and Deduction of the Chemical Structural Transformation 基于有效成分的不同生长年限泥石斛样品鉴定及化学结构转化推演

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-07-29 DOI: 10.1002/bmc.70178

Hanzhu Sun, Jiao Zhang, Zhengxu Hu, Jing Wang, Hongsui Rao, Yang Li, Tao Lin

{"title":"Identification of Dendrobium devonianum Samples With Different Growth Years Based on Active Ingredients and Deduction of the Chemical Structural Transformation","authors":"Hanzhu Sun, Jiao Zhang, Zhengxu Hu, Jing Wang, Hongsui Rao, Yang Li, Tao Lin","doi":"10.1002/bmc.70178","DOIUrl":"10.1002/bmc.70178","url":null,"abstract":"<div>\u0000 \u0000 <p>A rapid method was developed for the determination of nine active ingredients in <i>Dendrobium devonianum</i>, a specialty of Yunnan, China, using UHPLC–MS/MS. Principal component analysis was used for the systematic identification of <i>D. devonianum</i> samples with different growth years. The first three principal components completely covered the content information in the samples and effectively differentiated between <i>D</i>. <i>devonianum</i> samples with different growth years. Correlation analysis using Pearson's correlation coefficient showed a significant correlation between vanillic acid and the age of the samples, between 4-hydroxy benzaldehyde and eriodictyol, and between isoquercetin and hyperoside. The results of the volcano plot analysis showed that the up-regulated components among samples with different growth years were mainly naringin, apigenin, and 4-hydroxy benzaldehyde; the content of 4-hydroxy benzaldehyde varied the most among the <i>D</i>. <i>devonianum</i> samples with different growth years. Through a literature review and analysis of the changes in the active compounds in <i>D</i>. <i>devonianum</i> samples with different growth years, the chemical structural transformation of the active compounds in <i>D. devonianum</i> was deduced, and the optimal harvesting period of <i>D</i>. <i>devonianum</i> in Yunnan was determined to be the fourth year of growth.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144725602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reconstruction of Quality Marker System for Xiaoqinglong Decoction Using UHPLC-Q-Exactive Orbitrap MS, Network Pharmacology, and Molecular Docking 用uhplc - q - Orbitrap质谱、网络药理学和分子对接技术重建小青龙汤质量标记体系

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-07-28 DOI: 10.1002/bmc.70182

Bin Wang, Xiaojun Yu, Yihan Liu, Mingzhe Zhao, Xuejie Li, Luyao Huang, Jia Li

{"title":"Reconstruction of Quality Marker System for Xiaoqinglong Decoction Using UHPLC-Q-Exactive Orbitrap MS, Network Pharmacology, and Molecular Docking","authors":"Bin Wang, Xiaojun Yu, Yihan Liu, Mingzhe Zhao, Xuejie Li, Luyao Huang, Jia Li","doi":"10.1002/bmc.70182","DOIUrl":"10.1002/bmc.70182","url":null,"abstract":"<div>\u0000 \u0000 <p>Xiaoqinglong decoction (XQLT), a classical traditional Chinese medicine (TCM) formula, has demonstrated significant efficacy in the treatment of asthma. However, existing quality evaluation methods for this compound lack direct correlation with its therapeutic effects. This study aims to identify the chemical constituents of XQLT and establish quality markers (Q-Markers) associated with its efficacy in asthma treatment. UHPLC-Q-Exactive Orbitrap MS, assisted by comprehensive database analysis, was employed for the total component analysis of XQLT. This approach facilitated the evaluation of Q-Markers based on the principles of component testability, ingredient specificity, and formula compatibility environment. The identified compounds were subsequently subjected to network pharmacology, bioinformatics, and molecular docking analyses to further assess their component validity and traceability. A total of 143 chemical components were identified, including flavonoids, terpenoids, and steroids, organic acids, phenylpropanoids, alkaloids, sugars and derivatives, and other components. Seven key compounds, 18<i>β</i>-glycyrrhetinic acid, liquiritin, formononetin, liquiritigenin, 6-gingerol, neoisostegane, and 2-Methoxycinnamaldehyde, were proposed as potential Q-Markers for XQLT in asthma treatment. The establishment of Q-Markers not only enhances the understanding of XQLT's therapeutic mechanisms but also provides a scientific basis for its pharmacodynamic material foundation and quality control.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144716551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dissipation and Risk Assessment of a Combination Product of Chlorantraniliprole + Abamectin and Spirotetramat in Pomegranate Fruit and Juice 氯虫腈+阿维菌素与螺虫脒复合制剂在石榴果汁中的消散及风险评价

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-07-28 DOI: 10.1002/bmc.70174

Kaushikkumar D. Parmar, Paresh H. Rathod, Ravi L. Kalasariya, Mayur J. Pandya, Mitesh R. Patel, Suchi Chawla, Mukesh P. Patel

{"title":"Dissipation and Risk Assessment of a Combination Product of Chlorantraniliprole + Abamectin and Spirotetramat in Pomegranate Fruit and Juice","authors":"Kaushikkumar D. Parmar, Paresh H. Rathod, Ravi L. Kalasariya, Mayur J. Pandya, Mitesh R. Patel, Suchi Chawla, Mukesh P. Patel","doi":"10.1002/bmc.70174","DOIUrl":"10.1002/bmc.70174","url":null,"abstract":"<div>\u0000 \u0000 <p>Supervised field trials were conducted to understand the dissipation kinetics of the combination product chlorantraniliprole + abamectin and spirotetramat and its metabolites in pomegranate samples. QuEChERS-based methods were successfully used to analyze these pesticides from pomegranate samples with a correlation coefficient <i>R</i><sup>2</sup> > 0.99, accuracy 70–120%, and precision RSD ≤ 20% in all cases. The dissipation study showed that all the molecules followed first-order kinetics with half-lives ranging from 7.9 to 13.7 days, from 3.6 to 5.5 days, and from 1.4 to 1.6 days for chlorantraniliprole, abamectin, and spirotetramat, respectively. Dissipation kinetics and half-life were calculated in accordance with the residue definition of these pesticides. A pre-harvest (PHI) interval of 10 days is suggested. Risk assessment showed that the theoretical maximum daily intake (TMDI) was less than the acceptable daily intake (ADI) in all cases, suggesting that these pesticides can be used on pomegranate crops without any significant risk, provided good agricultural practices (GAP) are followed with a PHI of 10 days. Data from the present study can be used by national and international regulatory bodies for establishing maximum residue limits (MRLs) in pomegranate as well as can be extrapolated to establish PHI and MRLs for other berry fruits. However, there is a need to undertake such studies in other berry fruits in the future.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144716561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validation of a LC-MS/MS Assay for Rapid and Simultaneous Quantification of Cobicistat and Venetoclax in Human Plasma and Serum LC-MS/MS法快速同时定量人血浆和血清中Cobicistat和Venetoclax的验证

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-07-28 DOI: 10.1002/bmc.70179

Niels Westra, Kai van Hateren, Jos G. W. Kosterink, Marjolijn N. Lub-de Hooge, Thijs H. Oude Munnink, Daan J. Touw

{"title":"Validation of a LC-MS/MS Assay for Rapid and Simultaneous Quantification of Cobicistat and Venetoclax in Human Plasma and Serum","authors":"Niels Westra, Kai van Hateren, Jos G. W. Kosterink, Marjolijn N. Lub-de Hooge, Thijs H. Oude Munnink, Daan J. Touw","doi":"10.1002/bmc.70179","DOIUrl":"10.1002/bmc.70179","url":null,"abstract":"<p>Measuring cobicistat and venetoclax concentrations in human plasma and serum facilitates therapeutic drug monitoring (TDM) and pharmacokinetic (PK) boosting studies. Therefore, the objective of this study was to develop and validate a rapid LC-MS/MS analytical method for the simultaneous determination of cobicistat and venetoclax concentrations in plasma and serum. The method was validated according to EMA and FDA guidelines. Chromatographic separation was performed using a liquid chromatography (LC) system with a C18 column. The elution gradient involved two mobile phases: mobile phase A (ammonium formate) and mobile phase B (acetonitrile). The concentration range was 5–500 μg/L for cobicistat and 50–5000 μg/L for venetoclax. Accuracy and precision were within the required limits, with accuracy ranging from −5.9% to 2.4%, within-day precision from 1.2% to 4.8%, and between-day precision from 0.4% to 4.3%. Cobicistat and venetoclax were stable for at least 8 days under various storage and handling conditions. Clinical TDM samples showed mean concentrations ± standard deviation (SD) of 138.8 ± 123.3 μg/L for cobicistat and 1497.1 ± 1285.9 μg/L for venetoclax. The development and validation of this LC-MS/MS assay provide a reliable and efficient method for the simultaneous quantification of cobicistat and venetoclax in plasma and serum samples.</p>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bmc.70179","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144716559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening for the Breast Cancer-Associated Volatile Organic Compounds by GC–MS With Solid Phase Microextraction 固相微萃取-气相色谱-质谱法筛选乳腺癌相关挥发性有机物

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-07-27 DOI: 10.1002/bmc.70176

Yu Zhang, Li-Yang Shao, Yu Li, Jiayi Li, Lingyun Hui, Jing Zhang

{"title":"Screening for the Breast Cancer-Associated Volatile Organic Compounds by GC–MS With Solid Phase Microextraction","authors":"Yu Zhang, Li-Yang Shao, Yu Li, Jiayi Li, Lingyun Hui, Jing Zhang","doi":"10.1002/bmc.70176","DOIUrl":"10.1002/bmc.70176","url":null,"abstract":"<div>\u0000 \u0000 <p>The use of volatile organic compounds (VOCs) as noninvasive disease biomarkers has been explored as the basis for the diagnosis of human diseases. Although VOCs are of interest clinically, limited studies have been conducted because identifying disease-related VOCs and neglecting irrelevant variance in the data is still a formidable challenge. In our work, the VOCs in the headspace of breast cell medium and human exhaled gas were analyzed by solid-phase microextraction—gas chromatography—mass spectrometry. The common VOCs, which may be the breast cancer (BC)-associated VOCs, were identified by statistical analysis, including 1-butanol, cyclohexanone, nonanal, acetophenone, and benzyl alcohol. Then, after treatment of MCF-7 cells with letrozole, the levels of four VOCs were reduced. Five VOCs in exhaled breath of BC patients with drug therapy were decreased. It further proves that these VOCs are closely BC-associated VOCs. The study screened five BC-associated VOCs from in vitro cell VOCs and human exhaled breath, and provides basic research for VOCs that can be used in the screening of clinical diseases.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144714697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights Into the Mechanism of Action for Qingzhi Decoction for the Treatment of Non-Alcoholic Fatty Liver Disease via Regulation of the IRS/PI3K/Akt Signaling Pathway and the NRLP3 Inflammasome 清脂汤通过调控IRS/PI3K/Akt信号通路及NRLP3炎性体治疗非酒精性脂肪肝的作用机制

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-07-23 DOI: 10.1002/bmc.70156

Jiejun Yan, Qian Hou, Jing Liu, Weilan Li

{"title":"Insights Into the Mechanism of Action for Qingzhi Decoction for the Treatment of Non-Alcoholic Fatty Liver Disease via Regulation of the IRS/PI3K/Akt Signaling Pathway and the NRLP3 Inflammasome","authors":"Jiejun Yan, Qian Hou, Jing Liu, Weilan Li","doi":"10.1002/bmc.70156","DOIUrl":"10.1002/bmc.70156","url":null,"abstract":"<div>\u0000 \u0000 <p>The objective of this study is to explore the impacts of Qingzhi Decoction (QZT) in Non-alcoholic fatty liver disease (NAFLD) mice induced by a high-glucose and high-fat diet. UPLC-Orbitrap-Exploris MS characterized 156 bioactive components in QZT. Subsequently, an NAFLD model was established in ICR mice via 8-week feeding with a high-glucose and high-fat diet. The NAFLD mice were randomly divided into four groups. After 8 weeks of drug administration, compared with the control group, the model group showed significant NAFLD-related liver pathological changes, manifested as elevated blood lipid parameters (TC, TG, and LDL) and decreased HDL. Liver injury markers (ALT and AST) and serum inflammatory cytokines (IL-1β, IL-6, and TNF-α) were significantly increased. Molecular analysis indicated that the expression of p-IRs-1, PI3K, and p-Akt was decreased in liver tissue, while the expression of SREBP, NLRP3, caspase1, and GSDMD was increased. QZT treatment alleviated these changes, significantly improving NAFLD pathology, normalizing lipid profiles, and regulating the expression of these molecular markers (<i>p</i> < 0.01). QZT improved insulin resistance, regulated lipid metabolism, and reduced inflammatory response, and its mechanism might be associated with the regulation of IRs-1 and NLRP3 pathways.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144687854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Determination of Four Active Ingredients in Snow Chrysanthemum by HPLC With Determination of Its Antioxidant Activity in Vitro 高效液相色谱法同时测定雪菊中四种有效成分及其体外抗氧化活性

IF 1.7 4区医学

Biomedical Chromatography Pub Date : 2025-07-23 DOI: 10.1002/bmc.70172

Sai Xu, Bin Zhang, Shunyuan Li, Kaiqin Long

{"title":"Simultaneous Determination of Four Active Ingredients in Snow Chrysanthemum by HPLC With Determination of Its Antioxidant Activity in Vitro","authors":"Sai Xu, Bin Zhang, Shunyuan Li, Kaiqin Long","doi":"10.1002/bmc.70172","DOIUrl":"10.1002/bmc.70172","url":null,"abstract":"<p>We established a reliable high-performance liquid chromatography coupled with ultraviolet detector (HPLC-UV) method to simultaneously quantify and compare four indicator components in Kunlun Chrysanthemum (<i>Coreopsis tinctoria</i> Nutt.), Jiajin Chrysanthemum (<i>C. tinctoria</i> Nutt.) and Chrysanthemum buds (<i>C. tinctoria</i> Nutt.). We also aimed to evaluate the antioxidant activity of them in vitro. We showed that chlorogenic acid, 3,5-Odicaffeoylquinic acid, and Quercitrin concentrations were highest in Jiajin Chrysanthemum; Luteolin was highest in Chrysanthemum buds, and the four components were at an intermediate level in Kunlun Chrysanthemum. Antioxidant activity demonstrated IC<sub>50</sub> values for DPPH free radical scavenging of Kunlun Chrysanthemum, Jiajin Chrysanthemum, and Snow Chrysanthemum buds of 1.20, 1.33, and 2.41 mg/mL, respectively. The IC<sub>50</sub> values for ABTS radical scavenging were 2.53, 2.43, and 3.38 mg/mL, respectively. It showed differences in the four active ingredients of Snow Chrysanthemums from different origins and harvesting times. This HPLC-UV method accurately and rapidly determined the four active ingredients in Snow Chrysanthemums simultaneously, which may provide a quality control reference for Snow Chrysanthemums. Future studies exploring specific differences in cultivation, climate, harvesting, and drying of Snow Chrysanthemums to optimize its chemical composition would assist in developing its potential as a natural plant resource in health care.</p>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bmc.70172","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0