A Rapid and Simple Reversed-Phase HPLC–MS/MS Determination of Baloxavir in Rat Plasma and Its Application in Pharmacokinetic Study

IF 1.7 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2025-08-03 DOI:10.1002/bmc.70183

Peng Guoshuang, Wan Lili, Liu Xiang, Li Bei, Sun Yongbing

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Abstract

In this study, a novel RP-HPLC–MS/MS method was developed for the accurate quantification of baloxavir in rat plasma. The analytes were retained and separated on a C18 column, and a triple-quadrupole mass spectrometer equipped with an electrospray ionization (ESI) source was applied for quantification. Baloxavir could be well retained in the C18 column under the appropriate chromatographic conditions. Using a simple protein precipitation method, the extraction recovery was about 100%, and the ME could be negligible. The method demonstrated excellent linearity (r = 0.9995) across the concentration range of 1.59 ~ 3180.00 ng/mL for baloxavir. The precision and accuracy were within acceptable limits. The lowest limit of quantification (LLOQ) was determined to be 1.59 ng/mL. This simple method was validated and successfully applied to support the pharmacokinetics study after baloxavir was administered to the Sprague–Dawley rats.

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快速简便反相HPLC-MS /MS法测定大鼠血浆中巴洛韦的含量及其在药动学研究中的应用

本研究建立了一种新的RP-HPLC-MS /MS定量大鼠血浆中巴洛昔韦的方法。分析物在C18色谱柱上保留和分离，采用配备电喷雾电离（ESI）源的三重四极杆质谱仪进行定量。在合适的色谱条件下，巴洛昔韦在C18色谱柱中可以很好地保留。采用简单的蛋白质沉淀法，提取回收率约为100%，ME可以忽略不计。该方法在1.59 ~ 3180.00 ng/mL范围内具有良好的线性关系（r = 0.9995）。精密度和准确度均在可接受范围内。最低定量限为1.59 ng/mL。该方法经过验证，并成功应用于巴洛韦给药后大鼠药代动力学研究。

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.