Biomedical Chromatography最新文献

{"title":"Development and Validation of Isocratic RP-UHPLC Method for Assay of Fenofibrate Tablet","authors":"Jyoti Yadav,&nbsp;Saurav Kumar,&nbsp;Prabodh. C. Sharma,&nbsp;Parijat Pandey,&nbsp;Shivkant Sharma,&nbsp;Dhirender Kaushik","doi":"10.1002/bmc.70577","DOIUrl":"10.1002/bmc.70577","url":null,"abstract":"<div>\u0000 \u0000 <p>A method developed and validated for the assay of fenofibrate in tablet dosage form by isocratic RP-UHPLC–UV technique has been rapid and precise. The material was chromatographed on Shim-pack Velox C18 column (50 × 2.1 mm, 1.8 μm) at 40°C with isocratic mobile phase: acidified water/acetonitrile (60:40, v/v) at 0.4 mL/min and at a detection wavelength of 286 nm. The total run time was 6.0 min and fenofibrate eluted at ~2.4 min. The method was linear over 10–90 μg/mL (r² = 0.9995) with LOD/LOQ of 0.239/0.673 μg/mL. The precision and accuracy (RSD less than 2% and 96%–100% recovery) comply with ICH Q2(R1) requirements both intra-day and inter-day. The present UHPLC method significantly shortens the analysis time and solvent consumption compared with the reported HPLC methods (typical run time &gt; 8–20 min) without compromising of the sensitivity, which allows to use the present method for routine quality control of fenofibrate tablets.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148598417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Liposomal and Free Aqueous Extracts of Ceratonia siliqua: Evaluation of Phenolic Contents and Antimicrobial, Antimutagenic, Enzyme Inhibitory, and Antiproliferative Activities","authors":"Caglar Macit,&nbsp;Meltem Macit,&nbsp;Ozan Emre Eyupoglu,&nbsp;Elcin Ezgi Ozer,&nbsp;İnci Deniz,&nbsp;Gulsah Esen,&nbsp;Muhammed Hamitoglu","doi":"10.1002/bmc.70576","DOIUrl":"10.1002/bmc.70576","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Ceratonia siliqua</i> (carob) is a Mediterranean medicinal plant rich in phenolic contents but limited by poor bioavailability. To address this, an aqueous carob extract was formulated into liposomes and characterized. Phenolic profiles of the free (CBE) and the liposomal extracts (LCBE) were compared by the HPLC. Genotoxic safety and biological activities of both extracts were evaluated. HPLC analysis identified key phenolic acids in both formulations; while the concentration of gallic acid was determined as 23.5 ppm in CBE and 18.5 ppm in LCBE, caffeic acid levels were determined as 43.5 ppm in CBE and 24.5 ppm in LCBE. In addition, protocatechuic acid followed a similar trend, with concentrations of 55.1 ppm in CBE and 35.1 ppm in LCBE. Biologically, the LCBE showed enhanced antibacterial activity, particularly against <i>Escherichia coli</i>. The LCBE demonstrated stronger competitive inhibition of AChE and α-amylase when compared with the free extract, suggesting potential relevance for enzyme-modulating and glycemic support. In preliminary MTT-based cell viability screening, LCBE showed relatively greater activity than CBE in MCF-7 cells; however, this effect was weak, while PC-3 and MDA-MB-231 cells showed limited responsiveness under the tested conditions. Further selectivity and mechanistic studies should be done in vivo bioavailability/pharmacokinetics.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148590604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mass Spectrometry-Based Chromatographic and Computational Workflows for Biomarker and Therapeutic Target Discovery: A Comprehensive Review","authors":"K. Priya,&nbsp;R. Rajesh,&nbsp;T. Abhishek,&nbsp;M. Ankitha,&nbsp;Ramesh Kumar","doi":"10.1002/bmc.70575","DOIUrl":"10.1002/bmc.70575","url":null,"abstract":"<div>\u0000 \u0000 <p>The identification of biomarkers and therapeutic targets is essential to the success of precision medicine. However, to reliably identify them, one must employ a combination of analytical and computational methods. This article describes the importance of high-quality study design, strict adherence to preanalytical and quality assurance practices and high levels of quality assurance as well as the performance characteristics of multiple types of chromatography/mass spectrometry to achieve the best possible results for sensitive, selective biomarker profiling in various biological fluids such as plasma, serum, urine, cerebrospinal fluid (CSF), and tissues. In addition, it explores the key workflows used in preparing biological samples (i.e., solid-phase extraction/specialised physical or chemical extractions/derivatisation), software/data processing pipelines for peak analysis (i.e., XCMS/MZmine/OpenMS/MS-DIAL) and processes for the identification of compounds by combining spectral libraries (e.g., Human Metabolome Database [HMDB], National Institute of Standards and Technology [NIST] and FiehnLib) with in silico tools (e.g., SIRIUS, CSI: FingerID, CANOPUS). Finally, it discusses several ways in which artificial intelligence/machine learning can be applied to the field of mass spectrometry for peak detection and provides a comprehensive review of the requirements for regulatory-grade validation and the workflow for targeted/multiplexed/quantitative liquid chromatography/mass spectrometry (LC/MS) and gas chromatography/mass spectrometry (GC–MS) using stable isotope-labelled internal standards.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148590591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Saliva, Sweat, and Exhaled Breath as Alternative Specimens for Exercise Chemistry: Analytical Advantages, Limitations, and Comparative Progress","authors":"Jiale Pang","doi":"10.1002/bmc.70574","DOIUrl":"10.1002/bmc.70574","url":null,"abstract":"<div>\u0000 \u0000 <p>This critical narrative review evaluates saliva, sweat, and exhaled breath as noninvasive interface matrices for exercise chemistry rather than as general substitutes for blood or urine. It synthesizes peer-reviewed evidence on exercise-related sampling biology, analytical platforms, quantitative performance, validation limits, and translational readiness; because protocols and platforms remain heterogeneous, the evidence is interpreted qualitatively rather than pooled meta-analytically. Saliva is strongest for repeated neuroendocrine, autonomic, immune, oxidative, and short-latency metabolite measurements, but its interpretation depends on flow rate, timing, oral contamination, and normalization. Sweat is most mature for sweat rate and sodium/chloride loss monitoring and is uniquely compatible with wearable platforms, whereas sweat lactate, glucose, cortisol, and cytokines require stronger physiological validation before they can be treated as systemic markers. Exhaled breath offers second-scale access to volatile metabolism, substrate use, and airway responses, but breathomics remains instrumentally demanding and vulnerable to ventilation, diet, humidity, and ambient-air confounding. The review concludes that useful applications should match each analyte and platform to a defined exercise question, a matrix-specific validation model, and a practical decision context.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148583464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AQbD-Based Development and Optimization of a Stability-Indicating RP-HPLC Method for Imeglimin With Integration of Lean Six Sigma Principles","authors":"Vidhya K. Bhusari,&nbsp;Samiksha Ghorpade,&nbsp;Kamini Sethy","doi":"10.1002/bmc.70563","DOIUrl":"10.1002/bmc.70563","url":null,"abstract":"<div>\u0000 \u0000 <p>An accurate and green RP-HPLC stability indicating method has been developed for estimation of Imeglimin with an AQbD approach. Method optimization was done based on Box–Behnken design by changing factors like mobile phase, pH, and flow rate for optimum resolution and retention time. Lean Six Sigma (DMAIC) and statistical process control techniques ensured the consistency of the method. Water–methanol in the ratio of 65:35 (v/v) adjusted to pH 4.0 was used as the mobile phase at a flow rate of 0.8 mL/min, having an approximate retention time of 3.7 min. Method validation was performed as per the ICH guidelines, and the method exhibited a high correlation coefficient (<i>R</i>² = 0.9994), low %RSD (&lt; 2%), and good accuracy (99.45%–100.35%). LOD and LOQ were found to be 1.04 and 3.15 μg/mL, respectively. The results of forced degradation experiments showed significant degradation in the presence of alkaline, oxidative, and thermal stress conditions, whereas Imeglimin remained stable under photolytic stress conditions. HR-MS and FTIR studies indicated that N-dealkylation and N-demethylation were possible degradation pathways. The greenness assessment of the developed method was carried out using AGREE, GAPI, BAGI, and CLICK indices.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148577074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical Quality by Design-Based Reverse-Phase High-Performance Liquid Chromatography Method Development for Biomarker: A Comprehensive Review of Quality Assessment and Analytical Trends","authors":"Sakshi B. Patil,&nbsp;Shubhangi B. Sutar,&nbsp;Rachana Bhimanwar","doi":"10.1002/bmc.70573","DOIUrl":"10.1002/bmc.70573","url":null,"abstract":"<div>\u0000 \u0000 <p>Biomarkers play a crucial role in disease diagnosis and therapeutic monitoring, necessitating the development of reliable and robust analytical methods. Reverse-phase high-performance liquid chromatography (RP-HPLC) is widely employed for biomarker analysis due to its versatility, sensitivity and reproducibility. However, conventional method development approaches are based on trial-and-error strategies, leading to limited robustness and poor reproducibility. The Analytical Quality by Design (AQbD) framework has emerged as a systematic approach to overcome these limitations. This review critically evaluates reported RP-HPLC methods for biomarker analysis in the context of AQbD principles. Key elements, including Analytical Target Profile (ATP), Critical Quality Attributes (CQAs), Critical Method Parameters (CMPs), risk assessment, Design of Experiments (DoE) and Method Operable Design Region (MODR), are discussed. Analysis of selected studies indicates that most lack comprehensive AQbD implementation, particularly in terms of multivariate optimization, structured risk assessment and design space establishment. Common limitations such as coelution, sensitivity and inadequate robustness are highlighted, along with AQbD-based strategies to address these challenges. Additionally, recent trends, including the integration of machine learning and the adoption of Green and White Analytical Chemistry principles, are discussed to promote sustainable method development. Overall, AQbD offers a robust framework for improving analytical performance for biomarker analysis.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148583511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic Insights Into the Anti-Constipation Potential of Xiaogan Jianwei Formula: Integration of HPLC-Q-TOF MS/MS, Network Pharmacology, In Vivo Validation, and Gut Microbiota","authors":"Tao Li,&nbsp;Yuting Wang,&nbsp;Bin Du,&nbsp;Jiale Mao,&nbsp;Shuang Liu,&nbsp;Songquan Wu,&nbsp;Qiaoyong Ye,&nbsp;Na Lin,&nbsp;Shaochang Wu,&nbsp;Houxing Lei,&nbsp;Xiaoqin Zhang","doi":"10.1002/bmc.70567","DOIUrl":"10.1002/bmc.70567","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aims to explore the mechanism of action and potential bioactive constituents of Xiaogan Jianwei Formula (XG) in the treatment of constipation. The chemical constituents of XG and its absorbable components were characterized using HPLC-Q-TOF MS/MS, and network pharmacology was used to predict key targets and pathways, identifying the cAMP/PKA signaling pathway as a target pathway. In vivo experiments were performed to evaluate anti-constipation effects, including measurements of fecal water content, intestinal transit rate, MUC2 expression, cAMP/PKA/AQP3 signaling, and gut microbiota composition via 16S rRNA sequencing. In total, 138 chemical constituents were identified from XG, of which 32 components were absorbable; flavonoids were the main active ingredients. Pharmacological studies showed that XG significantly increased fecal water content and intestinal transit rate in loperamide-induced constipated rats without affecting physiological parameters. XG upregulated the expression of cAMP, PKA, p-PKA, and AQP3 and restored MUC2 expression. Moreover, XG improved gut microbiota diversity, enriched beneficial bacteria such as <i>Akkermansia</i>, and reduced pathogenic taxa like <i>Helicobacter</i> and Campylobacterota. XG alleviates constipation by regulating the cAMP/PKA/AQP3 signaling axis and modulating the gut microbiota. This study provides a scientific basis for the clinical application of XG and supports its development as an effective medicine for constipation.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148560808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Validation of a HPLC–MS/MS Method for Quantification of Andrographolide in Rat Plasma by Using Lithium Adduct Detection","authors":"Lei Mingdao,&nbsp;Huang Haijin,&nbsp;Luo Xiaoliang,&nbsp;Peng Guoshuang,&nbsp;Sun Yongbing","doi":"10.1002/bmc.70569","DOIUrl":"10.1002/bmc.70569","url":null,"abstract":"<div>\u0000 \u0000 <p>In this study, a simple high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS) method has been developed for quantification of andrographolide in rat plasma using andrographolide-lithium adduct as the precursor ion for the first time. The addition of lithium carbonate into the mobile phase could increase the mass response of andrographolide by approximately 100-fold. The analytes were retained and separated on a C18 column and analyzed using a triple quadrupole mass spectrometer equipped with an ESI source. Andrographolide exhibited excellent retention on the C18 column under optimized chromatographic conditions. A simple protein precipitation pretreatment resulted in high extraction recovery (approximately 100%) and negligible matrix effects. The method was linear over the concentration range of 2.00–1606.00 ng/mL. Both precision and accuracy were within acceptable limits. This simple HPLC–MS/MS method has been validated and successfully applied to support pharmacokinetic studies of andrographolide in Sprague–Dawley rats. The present study also provided a strategy for improving the mass response of drugs with low ionization efficiency, especially compounds from plants.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148547620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated GNPS Molecular Networking and Network Pharmacology Uncover Methylophiopogonanone B as a Novel Anti-Inflammatory Agent From Polygonatum cyrtonema Hua Targeting the SRC-PI3K-Akt Pathway","authors":"Yi Han,&nbsp;Renbin Li,&nbsp;Baobao Yao,&nbsp;Xuefeng Hou,&nbsp;Shixia Xu,&nbsp;Lizhen Yu,&nbsp;Jun Han,&nbsp;Huidan Huang,&nbsp;Di Cao","doi":"10.1002/bmc.70570","DOIUrl":"10.1002/bmc.70570","url":null,"abstract":"<div>\u0000 \u0000 <p>Inflammation is a defensive immune response to tissue damage or infection. <i>Polygonatum cyrtonema</i> Hua (<i>P</i>. <i>cyrtonema</i> Hua, PCH) is bioactive on immune homeostasis due to its rich components and reportedly plays a therapeutic role in the treatment and prevention of diabetes. Flavonoids of PCH possess anti-inflammatory properties, but their molecular mechanisms remain elusive. Here, chemical profiling of key flavonoids was conducted using UPLC-QTOF-MS/MS and the Global Natural Products Social Molecular Networking (GNPS) platform. Network pharmacology predicted potential targets and pathways, validated by molecular docking, surface plasmon resonance (SPR), and western blotting. Totally, 67 compounds were identified, with methylophiopogonanone B (MOB) as the key bioactive flavonoid. Although MOB's anti-inflammatory activity has been previously noted, the present study provides the first evidence that this effect may be mediated through targeting SRC and modulating the PI3K/AKT signaling axis. Core therapeutic targets were identified as SRC, TNF, and AKT1 by topological analysis of network pharmacology. Molecular docking and SPR confirmed strong MOB-SRC binding affinity. Western blotting revealed MOB dose-dependently inhibited LPS-induced phosphorylation of SRC, PI3K, and AKT1, without altering total protein levels. Furthermore, MOB significantly suppressed the phosphorylation of NF-κB pathway proteins IκB and p65, confirming the involvement of NF-κB as a downstream effector. In conclusion, this study integrates chemical profiling and network pharmacology with experimental validation to define the flavonoid composition of PCH, and is the first to implicate the SRC-PI3K-Akt pathway in MOB's anti-inflammatory action and providing novel evidence supporting the mechanism and use of PCH in contemporary diabetes treatment.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148547639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Artemisia argyi H.Lév. and Artemisia mongolica Based on GC Fingerprint and Chemometrics Analysis","authors":"Wenjing Zhang,&nbsp;Haiyan Li,&nbsp;Xiaowei Wang,&nbsp;Guiben Li,&nbsp;Yuan Yang,&nbsp;Haibo Wang","doi":"10.1002/bmc.70544","DOIUrl":"https://doi.org/10.1002/bmc.70544","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Artemisia argyi</i> Lévl. et Vant. and <i>Artemisia mongolica</i> (Fisch. ex Besser) Nakai are two distinct species of the genus <i>Artemisia</i> (Asteraceae). In some regions of China, <i>Artemisiae Mongolicae</i> Folium is frequently used as a substitute for <i>Artemisiae Argyi</i> Folium. These two herbal materials exhibit marked differences in chemical composition, which result in distinct therapeutic effects. Herein, gas chromatography (GC) fingerprinting and chemometrics were employed to compare their volatile constituents and establish an effective discrimination method. A standard GC fingerprinting procedure for <i>Artemisiae Argyi</i> Folium was established, yielding 21 common characteristic peaks. The similarity values of 20 batches of <i>Artemisiae Argyi</i> Folium were ≥ 0.894, whereas those of 12 batches of <i>Artemisiae Mongolicae</i> Folium were ≤ 0.812. Common peaks were further identified by GC-MS. Cluster analysis (CA), principal component analysis (PCA), and orthogonal partial least squares discriminant analysis (OPLS-DA) were applied to identify differential components. Multivariate analyses clearly separated the two species. Five volatile compounds with VIP &gt; 1.2 were selected as potential chemical markers to distinguish <i>Artemisiae Argyi</i> Folium from its adulterant <i>Artemisiae Mongolicae</i> Folium.</p>\u0000 </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"40 9","pages":""},"PeriodicalIF":1.7,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148533899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}