Study on the Mechanism of Lonicerae japonicae Caulis in the Treatment of Rheumatoid Arthritis Based on Transcriptomics and Energy Metabolomics

IF 1.7 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2025-07-23 DOI:10.1002/bmc.70175

Zhenzhen Xu, Jingyi Hou, Jing Li, Shijie Bi, Zewen Wang, Chaoqun Liu, Yanxia Liu, Bin Yu, Jiaye Tian, Yongjiang Yang, Liansheng Qiao, Yanjiang Qiao, Yanling Zhang

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引用次数: 0

Abstract

Rheumatoid arthritis (RA) is an autoimmune disorder characterized by inflammation, with current Western treatments focusing on symptom relief and disease progression control through antiinflammatory, analgesic, and immunosuppressive drugs. However, these therapies have significant side effects and limited long-term efficacy. Therefore, novel treatment options are urgently needed. Lonicerae japonicae Caulis (LJC), a traditional Chinese medicine, has alleviated RA symptoms such as joint pain and swelling. Despite its widespread use, the underlying mechanisms of its therapeutic effects remain poorly understood. This research aimed to uncover the mechanisms by which LJC intervenes in RA, using transcriptomics and energy metabolomics. RA was induced in rats via Type II collagen and incomplete adjuvant. LJC's effects were assessed through joint swelling, TNF-α levels, and histopathology. Mechanisms were analyzed using transcriptomics and metabolomics. The role of chlorogenic acid (CGA) was verified in LPS-induced RAW264.7 cells. LJC reduced joint swelling and TNF-α levels and inhibited synoviocyte proliferation and angiogenesis. Transcriptomics revealed LJC modulated RA pathways and inflammation. Metabolomics identified cis-aconitic acid as a key metabolite in energy metabolism. CGA was confirmed as the primary active component. LJC alleviates RA by inhibiting inflammation and modulating energy metabolism through multiple pathways.

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基于转录组学和能量代谢组学的金银花治疗类风湿关节炎的机制研究

类风湿关节炎（RA）是一种以炎症为特征的自身免疫性疾病，目前西方的治疗主要通过抗炎、镇痛和免疫抑制药物来缓解症状和控制疾病进展。然而，这些疗法有明显的副作用和有限的长期疗效。因此，迫切需要新的治疗方案。中药金银花（Lonicerae japonicae Caulis， LJC）可以缓解风湿性关节炎的关节疼痛和肿胀等症状。尽管其广泛使用，其治疗效果的潜在机制仍然知之甚少。本研究旨在利用转录组学和能量代谢组学揭示LJC干预RA的机制。采用ⅱ型胶原和不完全佐剂诱导大鼠RA。通过关节肿胀、TNF-α水平和组织病理学来评估LJC的作用。利用转录组学和代谢组学分析其机制。在lps诱导的RAW264.7细胞中证实了绿原酸（CGA）的作用。LJC降低关节肿胀和TNF-α水平，抑制滑膜细胞增殖和血管生成。转录组学显示LJC调节RA通路和炎症。代谢组学鉴定顺乌头酸是能量代谢的关键代谢物。CGA被确认为主要活性成分。LJC通过多种途径抑制炎症和调节能量代谢来缓解RA。

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.