Insights Into the Mechanism of Action for Qingzhi Decoction for the Treatment of Non-Alcoholic Fatty Liver Disease via Regulation of the IRS/PI3K/Akt Signaling Pathway and the NRLP3 Inflammasome

IF 1.7 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Jiejun Yan, Qian Hou, Jing Liu, Weilan Li
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Abstract

The objective of this study is to explore the impacts of Qingzhi Decoction (QZT) in Non-alcoholic fatty liver disease (NAFLD) mice induced by a high-glucose and high-fat diet. UPLC-Orbitrap-Exploris MS characterized 156 bioactive components in QZT. Subsequently, an NAFLD model was established in ICR mice via 8-week feeding with a high-glucose and high-fat diet. The NAFLD mice were randomly divided into four groups. After 8 weeks of drug administration, compared with the control group, the model group showed significant NAFLD-related liver pathological changes, manifested as elevated blood lipid parameters (TC, TG, and LDL) and decreased HDL. Liver injury markers (ALT and AST) and serum inflammatory cytokines (IL-1β, IL-6, and TNF-α) were significantly increased. Molecular analysis indicated that the expression of p-IRs-1, PI3K, and p-Akt was decreased in liver tissue, while the expression of SREBP, NLRP3, caspase1, and GSDMD was increased. QZT treatment alleviated these changes, significantly improving NAFLD pathology, normalizing lipid profiles, and regulating the expression of these molecular markers (p < 0.01). QZT improved insulin resistance, regulated lipid metabolism, and reduced inflammatory response, and its mechanism might be associated with the regulation of IRs-1 and NLRP3 pathways.

Abstract Image

Abstract Image

清脂汤通过调控IRS/PI3K/Akt信号通路及NRLP3炎性体治疗非酒精性脂肪肝的作用机制
本研究的目的是探讨清栀汤(QZT)对高糖高脂饮食诱导的非酒精性脂肪性肝病(NAFLD)小鼠的影响。UPLC-Orbitrap-Exploris质谱分析了枇杷叶中156种活性成分。随后,通过8周高糖高脂饮食,建立ICR小鼠NAFLD模型。将NAFLD小鼠随机分为四组。给药8周后,模型组与对照组比较,nafld相关肝脏病理改变明显,表现为血脂参数(TC、TG、LDL)升高,HDL降低。肝损伤标志物(ALT、AST)和血清炎症因子(IL-1β、IL-6、TNF-α)均显著升高。分子分析显示,肝组织中p-IRs-1、PI3K、p-Akt表达降低,SREBP、NLRP3、caspase1、GSDMD表达升高。QZT治疗可缓解这些变化,显著改善NAFLD病理,使脂质谱正常化,并调节这些分子标记物的表达(p < 0.01)。芪zt改善胰岛素抵抗,调节脂质代谢,降低炎症反应,其机制可能与调节IRs-1和NLRP3通路有关。
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来源期刊
Biomedical Chromatography
Biomedical Chromatography 生物-分析化学
CiteScore
3.60
自引率
5.60%
发文量
268
审稿时长
2.3 months
期刊介绍: Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.
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