{"title":"基于UPLC-Q/ tof - ms的血清代谢组学揭示了黄精对KK/Upj-Ay 2型糖尿病小鼠的潜在降糖机制","authors":"Hao Li, Shuhan Tang, Jinyue Huang, Yidan Sun, Yaqi Xu, Yige Wang, XinTong Li, Minyue Zhang, Rong Ma, Xianna Li, Hongying Xu, Hailong Zhang, Zhigang Wang","doi":"10.1002/bmc.70177","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p><i>Polygonatum sibiricum</i> (PS) is a traditional Chinese medicinal herb used as both food and medicinal material. PS is reportedly significantly effective in the treatment of type 2 diabetes mellitus (T2DM) in clinic. However, its pharmacological mechanism and endogenous antihyperglycemic markers remain unknown. This study investigated PS effects in KK/Upj-Ay 2 diabetic mice and revealed its potential antihyperglycemic mechanisms. Nontargeted serum metabolomics and pathophysiological detection characterized differentially abundant metabolites and determined changes in metabolic pathways. Fasting blood glucose decreased, while serum total cholesterol (TC), tumor necrosis factor-α (TNF-α), triglyceride (TG), and whole blood hemoglobin A1c (HbA1c) levels in kidney tissue homogenates were restored to different degrees following administration with PS. Observation of hematoxylin–eosin (HE) staining of the kidney and pancreas, periodic acid-Schiff (PAS) staining of the kidney, and immunohistochemistry of pancreatic insulin revealed that PS could reduce dysfunction in KK/Upj-A<sup>y</sup> 2 diabetic mice. Nontargeted serum metabolomics identified 37 T2DM biomarkers, 27 of which were significantly recovered by high-dose PS. Further pathway analysis revealed 11 key metabolic pathways involved in PS-mediated effects. These pathways regulate inflammation, lipid accumulation, oxidative stress, and insulin resistance, elucidating multitarget mechanisms by which PS ameliorates metabolic disturbances and exerts antihyperglycemic effects in KK/Upj-A<sup>y</sup> type 2 diabetic mice.</p>\n </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 9","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"UPLC-Q/TOF-MS-Based Serum Metabolomics Reveals the Potential Antihyperglycemic Mechanisms of Polygonatum sibiricum in KK/Upj-Ay Type 2 Diabetic Mice\",\"authors\":\"Hao Li, Shuhan Tang, Jinyue Huang, Yidan Sun, Yaqi Xu, Yige Wang, XinTong Li, Minyue Zhang, Rong Ma, Xianna Li, Hongying Xu, Hailong Zhang, Zhigang Wang\",\"doi\":\"10.1002/bmc.70177\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p><i>Polygonatum sibiricum</i> (PS) is a traditional Chinese medicinal herb used as both food and medicinal material. PS is reportedly significantly effective in the treatment of type 2 diabetes mellitus (T2DM) in clinic. However, its pharmacological mechanism and endogenous antihyperglycemic markers remain unknown. This study investigated PS effects in KK/Upj-Ay 2 diabetic mice and revealed its potential antihyperglycemic mechanisms. Nontargeted serum metabolomics and pathophysiological detection characterized differentially abundant metabolites and determined changes in metabolic pathways. Fasting blood glucose decreased, while serum total cholesterol (TC), tumor necrosis factor-α (TNF-α), triglyceride (TG), and whole blood hemoglobin A1c (HbA1c) levels in kidney tissue homogenates were restored to different degrees following administration with PS. Observation of hematoxylin–eosin (HE) staining of the kidney and pancreas, periodic acid-Schiff (PAS) staining of the kidney, and immunohistochemistry of pancreatic insulin revealed that PS could reduce dysfunction in KK/Upj-A<sup>y</sup> 2 diabetic mice. Nontargeted serum metabolomics identified 37 T2DM biomarkers, 27 of which were significantly recovered by high-dose PS. Further pathway analysis revealed 11 key metabolic pathways involved in PS-mediated effects. These pathways regulate inflammation, lipid accumulation, oxidative stress, and insulin resistance, elucidating multitarget mechanisms by which PS ameliorates metabolic disturbances and exerts antihyperglycemic effects in KK/Upj-A<sup>y</sup> type 2 diabetic mice.</p>\\n </div>\",\"PeriodicalId\":8861,\"journal\":{\"name\":\"Biomedical Chromatography\",\"volume\":\"39 9\",\"pages\":\"\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-07-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical Chromatography\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/bmc.70177\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Chromatography","FirstCategoryId":"3","ListUrlMain":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/bmc.70177","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
UPLC-Q/TOF-MS-Based Serum Metabolomics Reveals the Potential Antihyperglycemic Mechanisms of Polygonatum sibiricum in KK/Upj-Ay Type 2 Diabetic Mice
Polygonatum sibiricum (PS) is a traditional Chinese medicinal herb used as both food and medicinal material. PS is reportedly significantly effective in the treatment of type 2 diabetes mellitus (T2DM) in clinic. However, its pharmacological mechanism and endogenous antihyperglycemic markers remain unknown. This study investigated PS effects in KK/Upj-Ay 2 diabetic mice and revealed its potential antihyperglycemic mechanisms. Nontargeted serum metabolomics and pathophysiological detection characterized differentially abundant metabolites and determined changes in metabolic pathways. Fasting blood glucose decreased, while serum total cholesterol (TC), tumor necrosis factor-α (TNF-α), triglyceride (TG), and whole blood hemoglobin A1c (HbA1c) levels in kidney tissue homogenates were restored to different degrees following administration with PS. Observation of hematoxylin–eosin (HE) staining of the kidney and pancreas, periodic acid-Schiff (PAS) staining of the kidney, and immunohistochemistry of pancreatic insulin revealed that PS could reduce dysfunction in KK/Upj-Ay 2 diabetic mice. Nontargeted serum metabolomics identified 37 T2DM biomarkers, 27 of which were significantly recovered by high-dose PS. Further pathway analysis revealed 11 key metabolic pathways involved in PS-mediated effects. These pathways regulate inflammation, lipid accumulation, oxidative stress, and insulin resistance, elucidating multitarget mechanisms by which PS ameliorates metabolic disturbances and exerts antihyperglycemic effects in KK/Upj-Ay type 2 diabetic mice.
期刊介绍：
Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.
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