Biochemical Society transactions最新文献_第4页

Ubiquitin beyond the proteome: lipids, glycans, metabolites, nucleic acids, and an expanding molecular landscape. 蛋白质组之外的泛素：脂质、聚糖、代谢物、核酸和不断扩大的分子景观。

IF 4.4 3区生物学

Biochemical Society transactions Pub Date : 2026-06-24 DOI: 10.1042/BST20260081

Akinori Endo, Yukiko Yoshida

{"title":"Ubiquitin beyond the proteome: lipids, glycans, metabolites, nucleic acids, and an expanding molecular landscape.","authors":"Akinori Endo, Yukiko Yoshida","doi":"10.1042/BST20260081","DOIUrl":"10.1042/BST20260081","url":null,"abstract":"Ubiquitination is a versatile post-translational modification process in which the small globular protein ubiquitin is covalently attached to substrate proteins to generate diverse cellular signals. Although originally characterized by its role in proteasome-mediated protein degradation, ubiquitination is now recognized as a central regulator of numerous processes, including signaling, trafficking, and immunity. Canonical ubiquitination is mediated by a cascade of E1 (activating), E2 (conjugating), and E3 (ligase) enzymes that repeatedly conjugate ubiquitin molecules to lysine residues on substrate proteins, leading to the formation of polyubiquitin chains with distinct topologies. The modification is reversed by deubiquitinating enzymes. Notably, components of the ubiquitin system comprise approximately 7% of the human proteome, underscoring its importance in biological regulation. Recent advances have revealed the broad scope of ubiquitination. Ubiquitin was found to conjugate not only to lysine but also to serine, threonine, and cysteine, indicating its unexpected chemical flexibility. Furthermore, ubiquitination can be directed toward other post-translational modifications, particularly glycosylation and ADP-ribosylation, highlighting the extensive crosstalk between modification systems. Strikingly, lipids, sugars, metabolites, nucleic acids, and even synthetic small-molecule compounds have been identified as ubiquitinated substrates. The hypothesis that virtually all classes of molecules are targeted by ubiquitination has become increasingly plausible. Taken together, these findings redefine ubiquitination as a far more general modification process than previously appreciated. In this mini-review, we focus on recent progress in non-proteinaceous ubiquitination research, summarize emerging substrate classes, and discuss key challenges in elucidating the underlying mechanisms and physiological roles of this expanding modification landscape.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":"54 6","pages":"745-754"},"PeriodicalIF":4.4,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13270118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148222974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic regulation of pancreatic acinar cell homeostasis and plasticity. 胰腺腺泡细胞稳态和可塑性的转录组调控。

IF 4.4 3区生物学

Biochemical Society transactions Pub Date : 2026-06-24 DOI: 10.1042/BST20253124

Xin Zhang, Christopher Luo, Kevin Coughlin, Feng-Qian Li, Ken-Ichi Takemaru, Ledong Wan

引用次数: 0

The multifaceted roles of receptor tyrosine pseudokinases in cellular signalling. 受体酪氨酸假激酶在细胞信号传导中的多重作用。

IF 4.4 3区生物学

Biochemical Society transactions Pub Date : 2026-06-24 DOI: 10.1042/BST20260770

Emily C Park, Andrew P Thompson, James M Murphy, Lung-Yu Liang, Isabelle S Lucet

引用次数: 0

The primary cilium at the helm: gatekeeper of TGF-β superfamily signaling in development, homeostasis, and disease. 初级纤毛掌舵：TGF-β超家族信号在发育、体内平衡和疾病中的守门人。

IF 4.4 3区生物学

Biochemical Society transactions Pub Date : 2026-06-24 DOI: 10.1042/BST20253108

Cristian Herrera-Cid, Oskar Kaaber Thomsen, Linéa Désarbre, Rylee Blair Marin, Canan Doganli, Lotte Bang Pedersen, Lars Allan Larsen, Søren Tvorup Christensen

引用次数: 0

Lipid traffic: an enigmatic alliance between ORPs and a TMEM16-like protein at membrane contact sites. 脂质运输：orp与膜接触部位的tmem16样蛋白之间的神秘联盟。

IF 4.4 3区生物学

Biochemical Society transactions Pub Date : 2026-06-24 DOI: 10.1042/BST20250365

Alicia Fabbre, Guillaume Drin

{"title":"Lipid traffic: an enigmatic alliance between ORPs and a TMEM16-like protein at membrane contact sites.","authors":"Alicia Fabbre, Guillaume Drin","doi":"10.1042/BST20250365","DOIUrl":"10.1042/BST20250365","url":null,"abstract":"Lipid transfer proteins (LTPs) play a critical role in distributing lipids within eukaryotic cells. In yeast, Osh6 and Osh7, which belong to the oxysterol-binding protein-related protein family, transfer phosphatidylserine (PS) from the endoplasmic reticulum (ER) to the plasma membrane (PM) in exchange for phosphatidylinositol 4-phosphate (PI(4)P). These proteins localize at ER-PM contact sites by associating with Ist2, an ER-resident TMEM16-like protein that bridges the ER and PM via a long intrinsically disordered region (IDR). Recent studies have shown that this association ensures accurate PS transfer by concentrating Osh6 and Osh7 at the ER-PM interface while preserving their ability to access both membranes. However, it remains unclear how these LTPs function when bound to the Ist2 IDR, whose length far exceeds the ER-PM distance at contact sites, and why they do not integrate both the tethering and the PS/PI(4)P exchange functions, like their human homologs. Additionally, it has been revealed that Ist2 can transfer lipids across the ER membrane via a scramblase activity. Yet, whether and why this activity is coupled to the PS/PI(4)P exchange activity of Osh6 and Osh7 remains unknown. The Ist2-Osh6/7 system emerges as a fascinating model that integrates tethering, scramblase, and lipid exchange functions. Future studies of this system are likely to provide important insights into how lipid transfer processes are coordinated at membrane contact sites.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":"54 6","pages":"793-802"},"PeriodicalIF":4.4,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13286939/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148293251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of psuedouridine modification on human tRNA. 伪吡啶修饰对人tRNA的影响。

IF 4.4 3区生物学

Biochemical Society transactions Pub Date : 2026-06-24 DOI: 10.1042/BST20250258

Yasmin Stone, Ethan Morgan, Yong-Han Su, Ching-Ying Kuo, Ting-Yu Lin

{"title":"The impact of psuedouridine modification on human tRNA.","authors":"Yasmin Stone, Ethan Morgan, Yong-Han Su, Ching-Ying Kuo, Ting-Yu Lin","doi":"10.1042/BST20250258","DOIUrl":"10.1042/BST20250258","url":null,"abstract":"Transfer RNA (tRNA) is an important RNA in cells that decodes messenger RNA (mRNA) codons during protein translation to ensure correct amino acid sequences. The biogenesis of tRNA involves multiple processing steps to produce mature and functional molecules. Pseudouridine (Ψ), a derivative of uridine, is an abundant RNA modification and occurs at multiple positions within tRNAs. These modified sites are highly conserved across organisms. Classical biochemical studies have established that Ψ stabilises RNA-RNA interactions, but structural characterisations and molecular dynamics simulations reveal that Ψ can locally remodel tRNA architecture in ways that are dictated by where it is within tRNAs. Advances in transcriptome-wide Ψ mapping have uncovered additional modified sites beyond those previously described, with several novel sites appearing to be regulated in a cellular context-dependent manner. Furthermore, dysregulated pseudouridylation has been implicated in conditions ranging from cancer to inherited genetic disorders. Together, these developments reframe our understanding of Ψ from a well-established RNA stabiliser to a modification with roles far more dynamic, context-dependent, and clinically relevant than previously appreciated. The present review summarises and discusses the up-to-date developments in the impacts of pseudouridylation on human tRNA biogenesis, tRNA functions, and human health. More mechanistic questions remain open and will require further investigation. As pseudouridylation can also happen in mRNA and rRNA, exploring the interplay between these RNAs will be crucial for fundamental biology and advancing Ψ applications in biotechnology and biomedical uses.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":"54 6","pages":"755-767"},"PeriodicalIF":4.4,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13270119/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148249033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight into the relationship between type IV pilus function and biofilm formation. IV型菌毛功能与生物膜形成关系的探讨。

IF 4.4 3区生物学

Biochemical Society transactions Pub Date : 2026-06-24 DOI: 10.1042/BST20250151

Yafan Yu, Robert M Bauer, Rabab Mahdi, Kurt H Piepenbrink

{"title":"Insight into the relationship between type IV pilus function and biofilm formation.","authors":"Yafan Yu, Robert M Bauer, Rabab Mahdi, Kurt H Piepenbrink","doi":"10.1042/BST20250151","DOIUrl":"10.1042/BST20250151","url":null,"abstract":"Type IV pili (T4P) are protein nanofibers that can be extended and retracted from the surfaces of many bacterial taxa. They are involved in many aspects of bacterial physiology that differ between bacterial species, including surface motility, DNA uptake, and host-cell adherence, but genetically and structurally distinct type IV pilus systems from distantly related bacterial species have also been found to promote the formation of bacterial biofilms. The molecular mechanisms underpinning the promotion of biofilm remain an area of active investigation and may be both manifold and variable between type IV pilus systems. Two areas of recent interest are interactions between T4P and extracellular DNA and the relationship between surface-adhered biofilms and suspended aggregates. In the present review, we critically discuss the current state of knowledge of type IV pilus function and how these structures may interact with other biomolecules to influence the formation of multicellular bacterial communities. We examine the evidence for how alterations in DNA-binding, pilus retraction, and pilus composition have downstream effects on the formation of bacterial biofilms.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":"54 6","pages":"633-650"},"PeriodicalIF":4.4,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13234629/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148148315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-canonical subunit functions in multiprotein complexes: insights from the Mediator complex. 多蛋白复合物中的非规范亚基功能：来自中介复合物的见解。

IF 4.4 3区生物学

Biochemical Society transactions Pub Date : 2026-06-24 DOI: 10.1042/BST20260729

Alexis Verger, Didier Monté, Vincent Villeret

引用次数: 0

Pro-inflammatory cytokine-driven molecular signaling in skeletal muscle during cancer cachexia. 癌症恶病质期间骨骼肌促炎细胞因子驱动的分子信号传导。

IF 4.4 3区生物学

Biochemical Society transactions Pub Date : 2026-05-27 DOI: 10.1042/BST20260486

Benudhara Pati, Nibedita Nandy, Birendra Kumar Bindhani, Naresh Chandra Bal

{"title":"Pro-inflammatory cytokine-driven molecular signaling in skeletal muscle during cancer cachexia.","authors":"Benudhara Pati, Nibedita Nandy, Birendra Kumar Bindhani, Naresh Chandra Bal","doi":"10.1042/BST20260486","DOIUrl":"10.1042/BST20260486","url":null,"abstract":"Cancer cachexia is a multifactorial syndrome characterized by the progressive loss of muscle and fat, commonly observed among patients with cancer. It is very distinct from other skeletal muscle wasting such as sarcopenia and malnutrition and is known to reduce cancer treatment effectiveness. Cachexia progression is driven by a combination of factors, including hormonal dysregulation, anorexia, tumor-derived catabolic factors (in cancer cachexia), and systemic or muscular inflammation, all of which worsen overall muscle health. In this review, we will probe the role of pro-inflammatory cytokines, such as IL-6, IFN-γ, TNF-α, TGF-β, IL-1β, and IL-8, in driving the systemic inflammation and disruption of muscle metabolic homeostasis that support the development of cachexia. These cytokines may be produced from various organs, including the adipose depots that contribute to muscle wasting and metabolic dysfunction by disrupting the equilibrium between anabolic and catabolic processes. The ubiquitin-proteasome system, NF-κB, and JAK/STAT3 are important molecular pathways that mediate cytokine-induced catabolic signaling. The review further analyzes the context-dependent dual functions of these cytokines and the molecular mechanisms underlying the loss of their regulatory control during cancer progression. The limited success of current therapeutic approaches for cancer cachexia highlights the urgent need for evaluation of more targetable mechanisms for the treatments. Here, one of our main objectives is to probe whether suppression of pro-inflammatory cytokine signaling and activation of anti-inflammatory pathways can be utilized to modulate the tumor microenvironment, thereby countering cancer cachexia.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":"54 5","pages":"547-559"},"PeriodicalIF":4.4,"publicationDate":"2026-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13181344/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147947718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct or indirect: the crucial role of N-palmitate in hedgehog signaling. 直接或间接：n -棕榈酸盐在hedgehog信号传导中的关键作用。

IF 4.4 3区生物学

Biochemical Society transactions Pub Date : 2026-05-27 DOI: 10.1042/BST20260268

Janna Puschmann, Kay Grobe

引用次数: 0