Biochemical Society transactions最新文献

Structural insights into the membrane-bound proteolytic machinery of bacterial protein quality control. 从结构上洞察细菌蛋白质质量控制的膜结合蛋白水解机制。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-10-30 DOI: 10.1042/BST20231250

Rya Ero, Zhu Qiao, Kwan Ann Tan, Yong-Gui Gao

{"title":"Structural insights into the membrane-bound proteolytic machinery of bacterial protein quality control.","authors":"Rya Ero, Zhu Qiao, Kwan Ann Tan, Yong-Gui Gao","doi":"10.1042/BST20231250","DOIUrl":"10.1042/BST20231250","url":null,"abstract":"In bacteria and eukaryotic organelles of prokaryotic origin, ATP-dependent proteases are crucial for regulating protein quality control through substrate unfolding and degradation. Understanding the mechanism and regulation of this key cellular process could prove instrumental in developing therapeutic strategies. Very recently, cryo-electron microscopy structural studies have shed light on the functioning of AAA+ proteases, including membrane-bound proteolytic complexes. This review summarizes the structure and function relationship of bacterial AAA+ proteases, with a special focus on the sole membrane-bound AAA+ protease in Escherichia coli, FtsH. FtsH substrates include both soluble cytoplasmic and membrane-incorporated proteins, highlighting its intricate substrate recognition and processing mechanisms. Notably, 12 copies of regulatory HflK and HflC proteins, arranged in a cage-like structure embedded in the bacterial inner membrane, can encase up to 4 FtsH hexamers, thereby regulating their role in membrane protein quality control. FtsH represents an intriguing example, highlighting both its similarity to cytosolic AAA+ proteases with respect to overall architecture and oligomerization as well as its unique features, foremost its incorporation into a membrane-bound complex formed by HflK and HflC to mediate its function in protein quality control.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142457079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding the role of ten-eleven translocation family proteins in kidney diseases. 了解十-十一转位家族蛋白在肾脏疾病中的作用。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-10-30 DOI: 10.1042/BST20240291

Yuelin Zhang, Jiahui Li, Li Tan, Jun Xue, Yujiang Geno Shi

{"title":"Understanding the role of ten-eleven translocation family proteins in kidney diseases.","authors":"Yuelin Zhang, Jiahui Li, Li Tan, Jun Xue, Yujiang Geno Shi","doi":"10.1042/BST20240291","DOIUrl":"10.1042/BST20240291","url":null,"abstract":"Epigenetic mechanisms play a critical role in the pathogenesis of human diseases including kidney disorders. As the erasers of DNA methylation, Ten-eleven translocation (TET) family proteins can oxidize 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC), thus leading to passive or active DNA demethylation. Similarly, TET family proteins can also catalyze the same reaction on RNA. In addition, TET family proteins can also regulate chromatin structure and gene expression in a catalytic activity-independent manner through recruiting the SIN3A/HDAC co-repressor complex. In 2012, we reported for the first time that the genomic 5-hydroxymethylcytosine level and the mRNA levels of Tet1 and Tet2 were significantly downregulated in murine kidneys upon ischemia and reperfusion injury. Since then, accumulating evidences have eventually established an indispensable role of TET family proteins in not only acute kidney injury but also chronic kidney disease. In this review, we summarize the upstream regulatory mechanisms and the pathophysiological role of TET family proteins in major types of kidney diseases and discuss their potential values in clinical diagnosis and treatment.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142387606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histone H3 mutations and their impact on genome stability maintenance. 组蛋白 H3 突变及其对维持基因组稳定性的影响。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-10-30 DOI: 10.1042/BST20240177

Lucas D Caeiro, Ramiro E Verdun, Lluis Morey

引用次数: 0

The role of prokaryotic argonautes in resistance to type II topoisomerases poison ciprofloxacin. 原核生物箭毒在抗 II 型拓扑异构酶毒环丙沙星中的作用。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-10-30 DOI: 10.1042/BST20240094

Alina Galivondzhyan, Dmitry Sutormin, Vladimir Panteleev, Andrey Kulbachinskiy, Konstantin Severinov

引用次数: 0

How does CHD4 slide nucleosomes? CHD4 如何滑动核糖体？

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-10-30 DOI: 10.1042/BST20230070

Xavier J Reid, Yichen Zhong, Joel P Mackay

{"title":"How does CHD4 slide nucleosomes?","authors":"Xavier J Reid, Yichen Zhong, Joel P Mackay","doi":"10.1042/BST20230070","DOIUrl":"10.1042/BST20230070","url":null,"abstract":"Chromatin remodelling enzymes reposition nucleosomes throughout the genome to regulate the rate of transcription and other processes. These enzymes have been studied intensively since the 1990s, and yet the mechanism by which they operate has only very recently come into focus, following advances in cryoelectron microscopy and single-molecule biophysics. CHD4 is an essential and ubiquitous chromatin remodelling enzyme that until recently has received less attention than remodellers such as Snf2 and CHD1. Here we review what recent work in the field has taught us about how CHD4 reshapes the genome. Cryoelectron microscopy and single-molecule studies demonstrate that CHD4 shares a central remodelling mechanism with most other chromatin remodellers. At the same time, differences between CHD4 and other chromatin remodellers result from the actions of auxiliary domains that regulate remodeller activity by for example: (1) making differential interactions with nucleosomal epitopes such as the acidic patch and the N-terminal tail of histone H4, and (2) inducing the formation of distinct multi-protein remodelling complexes (e.g. NuRD vs ChAHP). Thus, although we have learned much about remodeller activity, there is still clearly much more waiting to be revealed.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disruptions to protein kinase A localization in adrenal pathology. 肾上腺病理学中的蛋白激酶 A 定位紊乱。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-10-30 DOI: 10.1042/BST20240444

Mitchell H Omar

引用次数: 0

Calcium signaling in mitochondrial intermembrane space. 线粒体膜间隙中的钙信号传递。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-10-30 DOI: 10.1042/BST20240319

Shanikumar Goyani, Shatakshi Shukla, Pooja Jadiya, Dhanendra Tomar

{"title":"Calcium signaling in mitochondrial intermembrane space.","authors":"Shanikumar Goyani, Shatakshi Shukla, Pooja Jadiya, Dhanendra Tomar","doi":"10.1042/BST20240319","DOIUrl":"10.1042/BST20240319","url":null,"abstract":"The mitochondrial intermembrane space (IMS) is a highly protected compartment, second only to the matrix. It is a crucial bridge, coordinating mitochondrial activities with cellular processes such as metabolites, protein, lipid, and ion exchange. This regulation influences signaling pathways for metabolic activities and cellular homeostasis. The IMS harbors various proteins critical for initiating apoptotic cascades and regulating reactive oxygen species production by controlling the respiratory chain. Calcium (Ca2+), a key intracellular secondary messenger, enter the mitochondrial matrix via the IMS, regulating mitochondrial bioenergetics, ATP production, modulating cell death pathways. IMS acts as a regulatory site for Ca2+ entry due to the presence of different Ca2+ sensors such as MICUs, solute carriers (SLCs); ion exchangers (LETM1/SCaMCs); S100A1, mitochondrial glycerol-3-phosphate dehydrogenase, and EFHD1, each with unique Ca2+ binding motifs and spatial localizations. This review primarily emphasizes the role of these IMS-localized Ca2+ sensors concerning their spatial localization, mechanism, and molecular functions. Additionally, we discuss how these sensors contribute to the progression and pathogenesis of various human health conditions and diseases.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safeguarding genomic integrity in beta-cells: implications for beta-cell differentiation, growth, and dysfunction. 保护β细胞基因组的完整性：对β细胞分化、生长和功能障碍的影响

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-10-30 DOI: 10.1042/BST20231519

Sneha S Varghese, Alessandro Giovanni Hernandez-De La Peña, Sangeeta Dhawan

{"title":"Safeguarding genomic integrity in beta-cells: implications for beta-cell differentiation, growth, and dysfunction.","authors":"Sneha S Varghese, Alessandro Giovanni Hernandez-De La Peña, Sangeeta Dhawan","doi":"10.1042/BST20231519","DOIUrl":"10.1042/BST20231519","url":null,"abstract":"The maintenance of optimal glucose levels in the body requires a healthy reserve of the insulin producing pancreatic beta-cells. Depletion of this reserve due to beta-cell dysfunction and death results in development of diabetes. Recent findings highlight unresolved DNA damage as a key contributor to beta-cell defects in diabetes. Beta-cells face various stressors and metabolic challenges throughout life, rendering them susceptible to DNA breaks. The post-mitotic, long-lived phenotype of mature beta-cells further warrants robust maintenance of genomic integrity. Failure to resolve DNA damage during beta-cell development, therefore, can result in an unhealthy reserve of beta-cells and predispose to diabetes. Yet, the molecular mechanisms safeguarding beta-cell genomic integrity remain poorly understood. Here, we focus on the significance of DNA damage in beta-cell homeostasis and postulate how cellular expansion, epigenetic programming, and metabolic shifts during development may impact beta-cell genomic integrity and health. We discuss recent findings demonstrating a physiological role for DNA breaks in modulating transcriptional control in neurons, which share many developmental programs with beta-cells. Finally, we highlight key gaps in our understanding of beta-cell genomic integrity and discuss emerging areas of interest.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biogenesis of omegasomes and autophagosomes in mammalian autophagy. 哺乳动物自噬过程中的奥米加体和自噬体的生物生成。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-10-30 DOI: 10.1042/BST20240015

Puck N Norell, Daniele Campisi, Jagan Mohan, Thomas Wollert

引用次数: 0

Mechanisms conferring bacterial cell wall variability and adaptivity. 赋予细菌细胞壁可变性和适应性的机制

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2024-10-30 DOI: 10.1042/BST20230027

Gabriel Torrens, Felipe Cava

引用次数: 0