Biochemical Society transactions最新文献_第2页

S-acylation in apoptotic and non-apoptotic cell death: a central regulator of membrane dynamics and protein function. 凋亡和非凋亡细胞死亡中的s -酰化：膜动力学和蛋白质功能的中心调节因子。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2025-04-29 DOI: 10.1042/BST20253012

Rojae Manhertz-Patterson, G Ekin Atilla-Gokcumen

{"title":"S-acylation in apoptotic and non-apoptotic cell death: a central regulator of membrane dynamics and protein function.","authors":"Rojae Manhertz-Patterson, G Ekin Atilla-Gokcumen","doi":"10.1042/BST20253012","DOIUrl":"10.1042/BST20253012","url":null,"abstract":"Protein lipidation is a collection of important post-translational modifications that modulate protein localization and stability. Protein lipidation affects protein function by facilitating interactions with cellular membranes, changing the local environment of protein interactions. Among these modifications, S-acylation has emerged as a key regulator of various cellular processes, including different forms of cell death. In this mini-review, we highlight the role of S-acylation in apoptosis and its emerging contributions to necroptosis and pyroptosis. While traditionally associated with the incorporation of palmitic acid (palmitoylation), recent findings indicate that other fatty acids can also participate in S-acylation, expanding its functional repertoire. In apoptosis, S-acylation influences the localization and function of key regulators such as Bcl-2-associated X protein and other proteins modulating their role in mitochondrial permeabilization and death receptor signaling. Similarly, in necroptosis, S-acylation of mixed lineage kinase domain-like protein (MLKL) with palmitic acid and very long-chain fatty acids enhances membrane binding and membrane permeabilization, contributing to cell death and inflammatory responses. Recent studies also highlight the role of S-acylation in pyroptosis, where S-acylated gasdermin D facilitates membrane localization and pore assembly upon inflammasome activation. Blocking palmitoylation has shown to suppress pyroptosis and cytokine release, reducing inflammatory activity and tissue damage in septic models. Collectively, these findings underscore S-acylation as a shared and important regulatory mechanism across cell death pathways affecting membrane association of key signaling proteins and membrane dynamics, and offer insights into the spatial and temporal control of protein function.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":"53 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functions and mechanisms of eukaryotic RNA-guided programmed DNA elimination. 真核生物rna引导的程序化DNA消除的功能和机制。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2025-04-29 DOI: 10.1042/BST20253006

Bozhidar-Adrian Stefanov, Mariusz Nowacki

{"title":"Functions and mechanisms of eukaryotic RNA-guided programmed DNA elimination.","authors":"Bozhidar-Adrian Stefanov, Mariusz Nowacki","doi":"10.1042/BST20253006","DOIUrl":"10.1042/BST20253006","url":null,"abstract":"Many eukaryotic organisms, from ciliates to mammals, employ programmed DNA elimination during their postmeiotic reproduction. The process removes specific regions from the somatic DNA and has broad functions, including the irreversible silencing of genes, sex determination, and genome protection from transposable elements or integrating viruses. Multiple mechanisms have evolved that explain the sequence selectivity of the process. In some cases, the eliminated sequences lack centromeres and are flanked by conserved sequence motifs that are specifically recognized and cleaved by designated nucleases. Upon cleavage, all DNA fragments that lack centromeres are lost during the following mitosis. Alternatively, specific sequences can be destined for elimination by complementary small RNAs (sRNAs) as in some ciliates. These sRNAs enable a PIWI-mediated recruitment of chromatin remodelers, followed up by the precise positioning of a cleavage complex formed from a transposase like PiggyBac or Tc1. Here, we review the known molecular interplay of the cellular machinery that is involved in precise sRNA-guided DNA excision, and additionally, we highlight prominent knowledge gaps. We focus on the modes through which sRNAs enable the precise localization of the cleavage complex, and how the nuclease activity is controlled to prevent off-target cleavage. A mechanistic understanding of this process could enable the development of novel eukaryotic genome editing tools.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":"53 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143963913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Exploring the influence of anionic lipids in the host cell membrane on viral fusion. 更正：探索宿主细胞膜中阴离子脂质对病毒融合的影响。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2025-04-28 DOI: 10.1042/BST20240833_COR

引用次数: 0

Extracellular membrane particles en route to the nucleus - exploring the VOR complex. 胞外膜颗粒在通往细胞核的途中-探索VOR复合体。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2025-04-24 DOI: 10.1042/BST20253005

Aurelio Lorico, Mark F Santos, Jana Karbanová, Denis Corbeil

{"title":"Extracellular membrane particles en route to the nucleus - exploring the VOR complex.","authors":"Aurelio Lorico, Mark F Santos, Jana Karbanová, Denis Corbeil","doi":"10.1042/BST20253005","DOIUrl":"https://doi.org/10.1042/BST20253005","url":null,"abstract":"Intercellular communication is an essential hallmark of multicellular organisms for their development and adult tissue homeostasis. Over the past two decades, attention has been focused on communication mechanisms based on various membrane structures, as illustrated by the burst of scientific literature in the field of extracellular vesicles (EVs). These lipid bilayer-bound nano- or microparticles, as vehicle-like devices, act as regulators in various biological and physiological processes. When EVs are internalized by recipient cells, their membrane and cytoplasmic cargoes can interfere with cellular activities, affecting pathways that regulate cell proliferation, differentiation, and migration. In cancer, EVs can transfer oncogenic factors, stimulate neo-angiogenesis and immunosuppression, reprogram stromal cells, and confer drug resistance traits, thereby remodeling the surrounding microenvironment. Although the mechanisms underlying EV biogenesis and uptake are now better understood, little is known about the spatiotemporal mechanism(s) of their actions after internalization. In this respect, we have shown that a fraction of endocytosed EVs reaches the nuclear compartment via the VOR (VAP-A-ORP3-Rab7) complex-mediated docking of late endosomes to the outer nuclear membrane in the nucleoplasmic reticulum, positioning and facilitating the transfer of EV cargoes into the nucleoplasm via nuclear pores. Here, we highlight the EV heterogeneity, the cellular pathways governing EV release and uptake by donor and recipient cells, respectively, and focus on a novel intracellular pathway leading to the nuclear transfer of EV cargoes. We will discuss how to intercept it, which could open up new avenues for clinical applications in which EVs and other small extracellular particles (e.g., retroviruses) are implicated.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":" ","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How do spherical bacteria regulate cell division? 球形细菌如何调节细胞分裂？

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2025-04-17 DOI: 10.1042/BST20240956

Félix Ramos-León, Kumaran S Ramamurthi

引用次数: 0

Manipulation of targeted protein degradation in plant biology. 植物生物学中靶蛋白降解的操纵。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2025-04-09 DOI: 10.1042/BST20230939

Marcela Rojas-Pierce, Sebastian Y Bednarek

{"title":"Manipulation of targeted protein degradation in plant biology.","authors":"Marcela Rojas-Pierce, Sebastian Y Bednarek","doi":"10.1042/BST20230939","DOIUrl":"10.1042/BST20230939","url":null,"abstract":"Inducible protein degradation systems are an important but untapped resource for the study of protein function in plant cells. Unlike mutagenesis or transcriptional control, regulated degradation of proteins of interest allows the study of the biological mechanisms of highly dynamic cellular processes involving essential proteins. While systems for targeted protein degradation are available for research and therapeutics in animals, there are currently limited options in plant biology. Targeted protein degradation systems rely on target ubiquitination by E3 ubiquitin ligases. Systems that are available or being developed in plants can be distinguished primarily by the type of E3 ubiquitin ligase involved, including those that utilize Cullin-RING ligases, bacterial novel E3 ligases, and N-end rule pathway E3 ligases, or they can be controlled by proteolysis targeting chimeras. Target protein ubiquitination leads to degradation by the proteasome or targeting to the vacuole, with both pathways being ubiquitous and important for the endogenous control of protein abundance in plants. Targeted proteolysis approaches for plants will likely be an important tool for basic research and to yield novel traits for crop biotechnology.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":"53 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143953262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into non-proteinaceous ubiquitination. 对非蛋白泛素化的洞察。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2025-04-03 DOI: 10.1042/BST20253029

Emily L Dearlove, Danny T Huang

引用次数: 0

Spo11: from topoisomerase VI to meiotic recombination initiator. Spo11：从拓扑异构酶VI到减数分裂重组引发剂。

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2025-04-02 DOI: 10.1042/BST20253019

Jon A Harper, George G B Brown, Matthew J Neale

{"title":"Spo11: from topoisomerase VI to meiotic recombination initiator.","authors":"Jon A Harper, George G B Brown, Matthew J Neale","doi":"10.1042/BST20253019","DOIUrl":"10.1042/BST20253019","url":null,"abstract":"Meiotic recombination is required to break up gene linkage and facilitate faithful chromosome segregation during gamete formation. By inducing DNA double-strand breaks, Spo11, a protein that is conserved in all meiotic organisms, initiates the process of recombination. Here, we chart the evolutionary history of Spo11 and compare the protein to its ancestors. Evolving from the A subunit of archaeal topoisomerase VI (Topo VI), a heterotetrameric type II topoisomerase, Spo11 appears to have evolved alongside meiosis and been present in the last eukaryotic common ancestor. There are many differences between Spo11 and TopVIA, particularly in regulation, despite similarities in structure and mechanism of action. Critical to its function as an inducer of recombination, Spo11 has an apparently amputated activity that, unlike topoisomerases, does not re-seal the DNA breaks it creates. We discuss how and why Spo11 has taken its path down the tree of life, considering its regulation and its roles compared with those of its progenitor Topo VI, in both meiotic and non-meiotic species. We find some commonality between different forms and orthologs of Spo11 in different species and touch upon how recent biochemical advances are beginning to finally unlock the molecular secrets hidden within this fundamental yet enigmatic protein.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":"53 2","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective direct motor cortical influence during naturalistic climbing in mice. 自然攀爬过程中运动皮层的选择性直接影响

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2025-03-28 DOI: 10.1101/2023.06.18.545509

Natalie Koh, Zhengyu Ma, Abhishek Sarup, Amy Claire Kristl, Mark Agrios, Margaret Young, Andrew Miri

{"title":"Selective direct motor cortical influence during naturalistic climbing in mice.","authors":"Natalie Koh, Zhengyu Ma, Abhishek Sarup, Amy Claire Kristl, Mark Agrios, Margaret Young, Andrew Miri","doi":"10.1101/2023.06.18.545509","DOIUrl":"10.1101/2023.06.18.545509","url":null,"abstract":"It remains poorly resolved when and how motor cortical output directly influences limb muscle activity through descending projections, which impedes mechanistic understanding of motor control. Here we addressed this in mice performing an ethologically inspired climbing behavior. We quantified the direct influence of forelimb primary motor cortex (caudal forelimb area, CFA) on muscles across the muscle activity states expressed during climbing. We found that CFA instructs muscle activity pattern by selectively activating certain muscles, while less frequently activating or suppressing their antagonists. From Neuropixels recordings, we identified linear combinations (components) of motor cortical activity that covary with these effects. These components differ partially from those that covary with muscle activity and differ almost completely from those that covary with kinematics. Collectively, our results reveal an instructive direct motor cortical influence on limb muscles that is selective within a motor behavior and reliant on a distinct neural activity subspace.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":"17 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11370436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80178576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tetraspanins affect membrane structures and the trafficking of molecular partners: what impact on extracellular vesicles? 四联蛋白影响膜结构和分子伙伴的运输：对细胞外囊泡有什么影响？

IF 3.8 3区生物学

Biochemical Society transactions Pub Date : 2025-03-26 DOI: 10.1042/BST20240523

Eric Rubinstein, Clotilde Théry, Pascale Zimmermann

{"title":"Tetraspanins affect membrane structures and the trafficking of molecular partners: what impact on extracellular vesicles?","authors":"Eric Rubinstein, Clotilde Théry, Pascale Zimmermann","doi":"10.1042/BST20240523","DOIUrl":"10.1042/BST20240523","url":null,"abstract":"Tetraspanins are a family of 33 proteins in mammals believed to play a crucial role in the compartmentalization of various associated proteins within cells and membranes. Recent studies have elucidated the structure of several tetraspanin members, revealing that while the four transmembrane domains typically adopt a cone-shaped configuration in crystals, other conformations are also possible. This cone-shaped structure may explain why tetraspanins are often enriched in curved and tubular cellular structures, such as microvilli, tunneling nanotubes, retraction fibers, or at the site of virus budding, and may contribute to the formation or maintenance of these structures. Tetraspanins have also been detected on midbody remnants and migrasomes, as well as on extracellular vesicles (EVs), for which CD9, CD81, and CD63 are widely used as markers. Although their impact on certain membrane structures and their ability to regulate the function and trafficking of associated proteins would suggest a potential role of tetraspanins either in EV formation or in regulating their protein composition, or both, efforts to characterize these roles have been complicated by conflicting results. In line with the interaction of certain tetraspanins with cholesterol, two recent studies have suggested that the presence or organization of oxysterols and cholesterol in EVs may be regulated by Tspan6 and CD63, respectively, paving the way for further research on the influence of tetraspanins on the lipid composition of EVs.","PeriodicalId":8841,"journal":{"name":"Biochemical Society transactions","volume":"0 0","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0