我该走还是该留:TFIIIC作为RNA聚合酶III转录的组装因子和屏障。

IF 4.3 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Wolfram Seifert-Davila, Maria Elize van Breugel, Fred van Leeuwen, Christoph W Müller
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引用次数: 0

摘要

真核生物基因转录调控的关键是一般转录因子(GTFs)与RNA聚合酶(rnap)的组装和相互作用,导致起始前复合物(PICs)的形成,这是转录激活的限速步骤。与RNAPII PIC组装涉及许多gtf、激活剂和共激活剂相比,RNAPIII PIC组装不那么复杂,主要涉及四种gtf TFIIIA、TFIIIB、TFIIIC和snRNA激活蛋白复合物,只有少数附加因子。rnapiii特异性GTF TFIIIC存在于I型和II型启动子中。一个重要的研究领域是TFIIIC与其启动子元件之间的动态相互作用,TFIIIC对这些元件的不同亲和力,以及TFIIIC内部的柔性连接体。此外,有证据表明TFIIIC可能发挥双重作用,在PIC形成过程中作为定位TFIIIB的组装因子,在rnapiii介导的转录过程中作为屏障。通过总结最近的结构、生化和基因组数据,本文探讨了RNAPIII特异性gtf,特别是TFIIIC,动态调节RNAPIII转录的机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Should I stay or should I go: TFIIIC as assembly factor and barrier in RNA polymerase III transcription.

Should I stay or should I go: TFIIIC as assembly factor and barrier in RNA polymerase III transcription.

Should I stay or should I go: TFIIIC as assembly factor and barrier in RNA polymerase III transcription.

Should I stay or should I go: TFIIIC as assembly factor and barrier in RNA polymerase III transcription.

Critical for the regulation of eukaryotic gene transcription is the assembly and interplay of general transcription factors (GTFs) with RNA polymerases (RNAPs), leading to the formation of pre-initiation complexes (PICs) as a rate-limiting step in transcription activation. Compared with RNAPII PIC assembly involving many GTFs, activators, and co-activators, RNAPIII PIC assembly is less complex, involving mainly the four GTFs TFIIIA, TFIIIB, TFIIIC, and snRNA activating protein complex with only a few additional factors. The RNAPIII-specific GTF TFIIIC is present in type I and II promoters. One prominent area of investigation has been the dynamic interaction between TFIIIC and its promoter elements, the varying affinities of TFIIIC toward these elements, and the flexible linker within TFIIIC. Additionally, evidence suggests that TFIIIC may play a dual role, acting as an assembly factor that positions TFIIIB during PIC formation and as a barrier during RNAPIII-mediated transcription. By summarizing recent structural, biochemical, and genomic data, this review explores the mechanisms by which RNAPIII-specific GTFs, with a focus on TFIIIC, dynamically regulate RNAPIII transcription.

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来源期刊
Biochemical Society transactions
Biochemical Society transactions 生物-生化与分子生物学
CiteScore
7.80
自引率
0.00%
发文量
351
审稿时长
3-6 weeks
期刊介绍: Biochemical Society Transactions is the reviews journal of the Biochemical Society. Publishing concise reviews written by experts in the field, providing a timely snapshot of the latest developments across all areas of the molecular and cellular biosciences. Elevating our authors’ ideas and expertise, each review includes a perspectives section where authors offer comment on the latest advances, a glimpse of future challenges and highlighting the importance of associated research areas in far broader contexts.
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