Biochemistry and Molecular Biology Education最新文献_第3页

An idea to explore: A systematic approach for solving plasmid double-digest puzzles. 一个值得探索的想法：解决质粒双酶切难题的系统方法

IF 1.2 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-08-02 DOI: 10.1002/bmb.21855

Aurora Callahan, Todd Smith

引用次数: 0

An effective Caenorhabditis elegans CRISPR training module for high school and undergraduate summer research experiences in molecular biology 针对高中生和本科生分子生物学暑期研究经历的有效 CaenorhabditiselegansCRISPR 培训模块

IF 1.2 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-07-27 DOI: 10.1002/bmb.21856

Carmen Herrera Sandoval, Christopher Borchers, Scott T Aoki

引用次数: 0

A primer for junior trainees: Recognition of RNA modifications by RNA-binding proteins 初级学员入门指南：RNA 结合蛋白对 RNA 修饰的识别。

IF 1.2 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-07-22 DOI: 10.1002/bmb.21854

Murphy Angelo, Yash Bhargava, Scott Takeo Aoki

{"title":"A primer for junior trainees: Recognition of RNA modifications by RNA-binding proteins","authors":"Murphy Angelo, Yash Bhargava, Scott Takeo Aoki","doi":"10.1002/bmb.21854","DOIUrl":"10.1002/bmb.21854","url":null,"abstract":"The complexity of RNA cannot be fully expressed with the canonical A, C, G, and U alphabet. To date, over 170 distinct chemical modifications to RNA have been discovered in living systems. RNA modifications can profoundly impact the cellular outcomes of messenger RNAs (mRNAs), transfer and ribosomal RNAs, and noncoding RNAs. Additionally, aberrant RNA modifications are associated with human disease. RNA modifications are a rising topic within the fields of biochemistry and molecular biology. The role of RNA modifications in gene regulation, disease pathogenesis, and therapeutic applications increasingly captures the attention of the scientific community. This review aims to provide undergraduates, junior trainees, and educators with an appreciation for the significance of RNA modifications in eukaryotic organisms, alongside the skills required to identify and analyze fundamental RNA–protein interactions. The pumilio RNA-binding protein and YT521-B homology (YTH) family of modified RNA-binding proteins serve as examples to highlight the fundamental biochemical interactions that underlie the specific recognition of both unmodified and modified ribonucleotides, respectively. By instilling these foundational, textbook concepts through practical examples, this review contributes an analytical toolkit that facilitates engagement with RNA modifications research at large.","PeriodicalId":8830,"journal":{"name":"Biochemistry and Molecular Biology Education","volume":"52 6","pages":"701-710"},"PeriodicalIF":1.2,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141733481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and identification of primary cells: A comprehensive primary cell culture experiment for graduate students 原代细胞的分离和鉴定：研究生原代细胞培养综合实验。

IF 1.2 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-07-17 DOI: 10.1002/bmb.21852

Jixiang Cao, Hao Chen, Qing Song, Hong Sun, Hua Yang, Yun Bai

{"title":"Isolation and identification of primary cells: A comprehensive primary cell culture experiment for graduate students","authors":"Jixiang Cao, Hao Chen, Qing Song, Hong Sun, Hua Yang, Yun Bai","doi":"10.1002/bmb.21852","DOIUrl":"10.1002/bmb.21852","url":null,"abstract":"Experimental teaching is an important part of postgraduate training in basic and clinical medicine. While primary cell isolation and identification are among the most important research techniques for medical graduate students, most graduate students do not understand and master these techniques before starting their research experience. In particular, many students lack training in this field, and high-quality teaching and learning materials are still very sparse. Here, we designed a practical experiment course for graduate students engaged in research. The target students usually have research projects involving primary cell culture in their future research, making the course highly applicable for the students. The lab exercise focused on the methods of primary cell isolation (including mechanical grinding method, explant culture method and enzymatic digestion method) and identification (including flow cytometry, immunofluorescence, and periodic acid-Schiff (PAS) staining). It aimed to help students master the conceptual, principle, technical, operation, and analytical skills related to primary cell culture and contributed to their foundation for future research. Students generally reflect that they have initially mastered the isolation and identification of primary cell culture as a result of the course. Student feedback also indicates significantly increased confidence in the practical application of primary cell culture in the future. Here, we provide our experience for others who may want to implement similar courses.","PeriodicalId":8830,"journal":{"name":"Biochemistry and Molecular Biology Education","volume":"52 6","pages":"648-655"},"PeriodicalIF":1.2,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of an instructional team's feedback on an instructor's teaching practices in a Biology of Cancer course 在《癌症生物学》课程中，教学团队的反馈对教师教学实践的影响。

IF 1.2 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-07-17 DOI: 10.1002/bmb.21850

Patricia Moreira, Lisa Rezende, Ashton Goodell, Paul Blowers, Lisa Elfring, Vicente Talanquer

{"title":"Impact of an instructional team's feedback on an instructor's teaching practices in a Biology of Cancer course","authors":"Patricia Moreira, Lisa Rezende, Ashton Goodell, Paul Blowers, Lisa Elfring, Vicente Talanquer","doi":"10.1002/bmb.21850","DOIUrl":"10.1002/bmb.21850","url":null,"abstract":"The effective implementation of evidence-based teaching (EBT) in large college courses benefits from the successful use of instructional teams. An instructional team's feedback allows instructors to act based on evidence of student learning, addressing students' needs. This feedback may be particularly important for novice instructors or experienced instructors teaching a class for the first time. This study sought to characterize the nature of an instructional team's feedback as well as its influence on the decisions and actions of a seasoned instructor teaching a new class. Instructional team members provided feedback in the form of anticipations, noticings, and suggestions. Anticipations and suggestions seemed to have the largest impact on the instructor's decisions and actions, while noticings, despite providing insights into student thinking, had a smaller effect. Our findings indicate that an instructional team can provide valuable feedback to instructors when team members have an opportunity to meaningfully participate in the planning and teaching processes.","PeriodicalId":8830,"journal":{"name":"Biochemistry and Molecular Biology Education","volume":"52 6","pages":"621-632"},"PeriodicalIF":1.2,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From primers to pipettes: An immersive course introducing high school students to qPCR for quantifying chemical defense gene expression 从引物到移液管：向高中生介绍量化化学防御基因表达的 qPCR 的沉浸式课程。

IF 1.2 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-07-16 DOI: 10.1002/bmb.21851

Zeke T. Spooner, Angela M. Encerrado-Manriquez, Tina T. Truong, Sascha C. T. Nicklisch

{"title":"From primers to pipettes: An immersive course introducing high school students to qPCR for quantifying chemical defense gene expression","authors":"Zeke T. Spooner, Angela M. Encerrado-Manriquez, Tina T. Truong, Sascha C. T. Nicklisch","doi":"10.1002/bmb.21851","DOIUrl":"10.1002/bmb.21851","url":null,"abstract":"We created a 2-week, dual-module summer course introducing high school students to environmental toxicology by teaching them quantitative polymerase chain reaction (qPCR) as a way to quantify gene expression of chemical defense proteins in response to exposure to environmental pollutants. During the course, students are guided through the various stages of a successful qPCR experiment: in silico primer design and quality control, total RNA extraction and isolation, cDNA conversion, primer test PCR, and evaluation of results via agarose gel electrophoresis or UV/Vis spectra. The course combines lectures, discussions, and demonstrations with dry and wet laboratory sections to give students a thorough understanding of the scope, utility, and chemical principles of qPCR. At the end of the course, the students are taught how to analyze qPCR data and are encouraged to discuss their findings with other classmates to evaluate their hypotheses and assess possible sources of error. This course was designed to be easily adaptable to multiple test species, chemical exposures, and genes of interest. To explore both terrestrial and aquatic toxicology, the students use honey bees (Apis mellifera) and mosquitofish (Gambusia affinis) as test organisms, as well as ABC-type efflux transporters, antioxidant enzymes, and cytochrome P450 enzymes as endpoints for assessing gene expression. We share this course setup and applied protocols to encourage others to design and offer similar courses that give high school students a hands-on introduction to a broad swath of environmental toxicology research and an opportunity to develop scientific skills necessary for university-level research.","PeriodicalId":8830,"journal":{"name":"Biochemistry and Molecular Biology Education","volume":"52 6","pages":"633-647"},"PeriodicalIF":1.2,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bmb.21851","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transforming undergraduate education: Meeting report from the 2023 ASBMB summer education conference 改革本科教育：2023年ASBMB夏季教育大会的会议报告。

IF 1.2 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-07-14 DOI: 10.1002/bmb.21848

Pamela S. Mertz, Odutayo O. Odunuga, Celeste N. Peterson, Joseph J. Provost

引用次数: 0

Antibiotic resistance: Assessing knowledge and misconceptions among Portuguese students and science teachers 抗生素耐药性：评估葡萄牙学生和科学教师的知识和错误观念。

IF 1.2 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-07-11 DOI: 10.1002/bmb.21849

Maria Manuel Azevedo, Ana Gonçalves, Nuno S. Osório, Fátima Baltazar

{"title":"Antibiotic resistance: Assessing knowledge and misconceptions among Portuguese students and science teachers","authors":"Maria Manuel Azevedo, Ana Gonçalves, Nuno S. Osório, Fátima Baltazar","doi":"10.1002/bmb.21849","DOIUrl":"10.1002/bmb.21849","url":null,"abstract":"Antimicrobial resistance poses one of the most significant medical challenges for humanity. The current burden is overwhelming and is projected to escalate rapidly, with predictions for 2050 indicating 10 million deaths per year due to antibiotic-resistant microorganisms. Enhancing public awareness and education on this topic is crucial in efforts to mitigate this issue. In our study, we translated an existing questionnaire on antimicrobial resistance into Portuguese, validated it, and applied it between December 2020 and March 2021 to a group of Portuguese students (n = 112) and science teachers (n = 95). A majority of the students surveyed (65.1%) incorrectly believed that antibiotics could treat colds/flus. As anticipated, the teachers outperformed the students in the questionnaire. However, difficulties with this topic were evident in both groups. Most notably, the misconception that the human body becomes resistant to antibiotics was prevalent among most participants (77.0% of students and 68.4% of teachers). Consistent with previous studies in other populations and geographic locations, our research reveals a worrying lack of knowledge about antimicrobial resistance among Portuguese students and science teachers. Consequently, it is deemed urgent to implement effective measures to raise awareness and educate on this topic.","PeriodicalId":8830,"journal":{"name":"Biochemistry and Molecular Biology Education","volume":"52 6","pages":"612-620"},"PeriodicalIF":1.2,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141578910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of Serum Adipsin Level with Insulin Resistance and Inflammatory Markers in Newly Diagnosed Type two Diabetes Mellitus Patients. 新诊断的二型糖尿病患者血清 Adipsin 水平与胰岛素抵抗和炎症标志物的关系

IF 1.5 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-07-01 Epub Date: 2023-03-30 DOI: 10.1007/s12291-023-01126-3

Somya Singh, Sartaj Hussain, Suraj Singh Yadav, Nitesh Pati Tiwari, Kauser Usman, Kamal Kumar Sawlani, Sanjay Khattri

{"title":"Association of Serum Adipsin Level with Insulin Resistance and Inflammatory Markers in Newly Diagnosed Type two Diabetes Mellitus Patients.","authors":"Somya Singh, Sartaj Hussain, Suraj Singh Yadav, Nitesh Pati Tiwari, Kauser Usman, Kamal Kumar Sawlani, Sanjay Khattri","doi":"10.1007/s12291-023-01126-3","DOIUrl":"10.1007/s12291-023-01126-3","url":null,"abstract":"Adipsin is an anti-inflammatory adipokines and its altered level was seen in obesity and type II DM. Our study investigated the clinical significance of serum adipsin levels as a risk marker for type 2 diabetes and its relationships with insulin resistance and various adipo-cytokines. The study included 110 treatment-naïve T2DM cases and 100 controls of similar age and gender from northern India. Clinical, biochemical, and anthropometric characteristics were all profiled. Serum adipo-cytokines were measured using ELISA methods. Adipsin was significantly inversely correlated with body mass index (BMI), waist circumference, fasting plasma glucose, glycated haemoglobin (HbA1C), total cholesterol (TC), triglyceride (TG), homeostasis model assessment-estimated insulin resistance (HOMA-IR), tumour necrosis factor- α (TNF-α) and interleulin-6 (IL-6) and positively correlated with high-density lipoprotein cholesterol (HDL-C) and homeostasis model assessment of β-cell function (HOMA-B) (P < 0.05). T2DM occurrence decreased with increasing concentration of adipsin with an odds ratio (OR) of 0.68 (95% CI = 0.58-0.79), P < 0.001. The area under curve (95% CI) for adipsin was 0.70 (0.63 to 0.76) with P < 0.001. The best cutoff value for adipsin to predict T2DM was < 5.50 µg/ml with 47.27% sensitivity and 82.00% specificity. FPG and WC were both independent predictors of serum adipsin levels. Our findings showed that high adipsin levels reduced the likelihood of T2DM and emerged as a potential risk marker in the prediction of T2DM.Graphical abstract: ","PeriodicalId":8830,"journal":{"name":"Biochemistry and Molecular Biology Education","volume":"40 1","pages":"415-420"},"PeriodicalIF":1.5,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11239611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73097371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feasible and economic DIY procedures of a hand-held fluorescence detector set—FluorDetector to assist laboratory course development 手持式荧光探测器--FluorDetector 的可行和经济 DIY 程序，以协助实验室课程开发。

IF 1.2 4区教育学

Biochemistry and Molecular Biology Education Pub Date : 2024-06-25 DOI: 10.1002/bmb.21846

Bo Ding, Ju Wang, Yaxin Mao, Tian Meng, Yumei Luo, Xiaomei Xu, Zhanqing Lv, Mian Zhou, Qiuying Yang

{"title":"Feasible and economic DIY procedures of a hand-held fluorescence detector set—FluorDetector to assist laboratory course development","authors":"Bo Ding, Ju Wang, Yaxin Mao, Tian Meng, Yumei Luo, Xiaomei Xu, Zhanqing Lv, Mian Zhou, Qiuying Yang","doi":"10.1002/bmb.21846","DOIUrl":"10.1002/bmb.21846","url":null,"abstract":"Fluorescence-related experimental techniques play an important role in biochemistry, molecular biology, and cell biology. However, fluorescence-related experiments are rarely included in the laboratory courses of most Chinese universities. This is mainly due to the conflict between large class size (50–60 students in one room) and funding/space limitations to purchase and accommodate enough fluorescence detection equipment. Here, we proposed feasible and economical Do It Yourself (DIY) procedures of a hand-held fluorescence detector set—FluorDetector to support the development of laboratory courses. Tested on several samples, clear fluorescence signals could be directly observed by FluorDetector and photographed with a smartphone. In addition, FluorDetector was able to turn a conventional stereomicroscope into a fluorescence stereomicroscope, detecting fluorescence signals with clean background. FluorDetector is easy to make with a 3D printer, with an extremely low cost ($200 each) when compared with a commercial fluorescence microscope or fluorescence stereomicroscope, and almost as sensitive as a microplate reader in measuring fluorescence. Therefore, FluorDetector is a possible strategy to solve the problem and help to integrate fluorescence-related experimental modules in laboratory courses.","PeriodicalId":8830,"journal":{"name":"Biochemistry and Molecular Biology Education","volume":"52 5","pages":"580-587"},"PeriodicalIF":1.2,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bmb.21846","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141454904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"教育学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0