Biocatalysis and agricultural biotechnology最新文献

{"title":"Innovative biological control strategies for managing peach tree canker disease caused by Cytospora leucostoma","authors":"Mohammed Jamal Jameel ,&nbsp;Muhammed Zrar Bakir ,&nbsp;Hayman Kakakhan Awla ,&nbsp;Majid Hassan Mustafa ,&nbsp;Tavga Sulaiman Rashid","doi":"10.1016/j.bcab.2025.103725","DOIUrl":"10.1016/j.bcab.2025.103725","url":null,"abstract":"<div><div>Peach Tree Canker, caused by <em>Cytospora leucostoma</em>, has traditionally been regarded as one of the most severe fungal diseases causing serious economic loss in peach production worldwide. Traditional chemical control methods against this pathogen are often inefficient and threaten environmental and human health. Within the frame of the present study, new alternative methods for biological control of peach tree canker have been investigated, taking advantage of useful microorganisms and plant extracts. Other experiments were conducted to assess the capability of bacterial antagonists to suppress the growth of <em>C. leucostoma</em> in combination with the antifungal properties of different plant extracts. <em>Bacillus subtilis, Pseudomonas fluorescens</em>, and <em>Aspergillus flavus</em> showed 100 % inhibition of <em>C. leucostoma</em> in vitro with 80 % inhibition by Thuja extract. Field experiments showed that applications of these biocontrol agents in separated and combined greatly enhanced canker wound healing by 100 % of infected trees, significantly improving general plant health. The results showed that this combined treatment of bacterial and fungus antagonists with Thuja extract synergistically enhanced the plant's defense mechanisms. It concludes that the biocontrol strategy is an eco-friendly and sustainable alternative to chemical fungicides for an efficient approach to managing peach tree canker in contemporary orchards.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103725"},"PeriodicalIF":3.8,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144852665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sourcing the vaccine adjuvant QS-21 and related saponins from cell cultures of Quillaja lancifolia (Brazilian soap tree)","authors":"Yve VS. Magedans ,&nbsp;Anna CA. Yendo ,&nbsp;Eliane Zachert ,&nbsp;Maria CS. Klauss ,&nbsp;Samuel Cibulski ,&nbsp;Fernando L. Silveira ,&nbsp;Yuri M. do Nascimento ,&nbsp;Fernanda M. da Silva ,&nbsp;Marcelo S. Silva ,&nbsp;Paulo M. Roehe ,&nbsp;Mariana Koetz ,&nbsp;José Ângelo S. Zuanazzi ,&nbsp;Arthur G. Fett-Neto","doi":"10.1016/j.bcab.2025.103724","DOIUrl":"10.1016/j.bcab.2025.103724","url":null,"abstract":"<div><div>Soapbark tree saponins, notably QS-21, are highly required as adjuvants in commercial human and veterinary vaccines. Current industrial sources of these specialized metabolites rely on the bark of adult trees, posing challenges in terms of supply speed and sustainability. To address this matter, cell cultures of <em>Quillaja lancifolia</em> (Brazilian soap tree) were evaluated as a new source of immunoadjuvant saponins. Cell cultures were successfully established, characterized, and elicited. Saponin accumulation followed biomass increase. Carbon source modulated saponin profiles. Immunoadjuvant activity of cell culture-derived saponin aqueous extract was confirmed in murine model of influenza vaccine. The presence of QS-21 in cell cultures was verified by liquid chromatography and mass spectrometry. QS-21 concentration ranged from 0.01 to 0.1 % of the unelicited dry mass. A three-fold increase in QS-21 concentration was achieved by elicitation with salicylic acid. This is the first report on the production of QS-21 in cell cultures of <em>Q. lancifolia</em> and one of the top yields registered to date from a fully defined tree-free source for the genus. These cell cultures represent a new sustainable source of this high value complex triterpene in increasing demand by the vaccine industry.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103724"},"PeriodicalIF":3.8,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144888657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the in vitro antimicrobial activity of the crude extract and solvent fractions of Euphorbia dumalis against selected bacteria and fungi","authors":"Abebe Dagne ,&nbsp;Shewaneh Ayele ,&nbsp;Getnet Tadege ,&nbsp;Dehnnet Abebe","doi":"10.1016/j.bcab.2025.103723","DOIUrl":"10.1016/j.bcab.2025.103723","url":null,"abstract":"<div><h3>Background</h3><div>Infectious diseases pose a major threat to public health worldwide because antimicrobials are unavailable, unaffordable, and resistant. Due to this, searching for medicinal plants is a useful task. In this study, crude extracts and fractions of <em>Euphorbia dumalis</em> were tested for their antibacterial and antifungal activity.</div></div><div><h3>Methods</h3><div>Agar-well diffusion and broth microdilution techniques were employed to evaluate the antimicrobial activity. The crude extract and solvent fractions were obtained using cold maceration and separatory funnel.</div></div><div><h3>Results</h3><div>At 200 mg/ml, the highest inhibition zone diameter (16.67 ± 0.33) was observed, while the least inhibition zone (1 mm) was recorded against gram-negative bacteria like <em>E. coli</em> and <em>P. aeruginosa</em>. At a concentration of 0.5 mg/ml, <em>S. epidermidis, E. coli,</em> and <em>E. faecalis</em> were susceptible to the crude extract, while <em>S. aureus</em> and <em>K. pneumonia</em> had inhibitory activity at 1 mg/ml. Butanol and aqueous fractions were the most active against <em>S. aureus</em> and <em>E. faecalis</em> at 0.25 mg/ml. Among the three fungal strains, the root extract displayed the highest inhibition zone diameter (31.33 ± 0.67 mm) against <em>T. mentagrophytes</em> at a concentration of 200 mg/ml. The crude extract was the most active against <em>C. albicans</em> at 2 mg/ml, while this fungus and <em>T. mentagrophytes</em> were the most susceptible to the butanol fraction with a MIC of 1 mg/ml.</div></div><div><h3>Conclusions</h3><div>The crude extract showed better antibacterial and antifungal activity than the hexane and chloroform fractions against all tested microorganisms. Thus, the present study supports the traditional use of <em>Euphorbia dumalis</em>.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103723"},"PeriodicalIF":3.8,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144840873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A reliable and effective method for regenerating Uraria picta (Jacq.) in vitro using nodal explants sourced from mature seed-derived seedlings","authors":"Samarth A. Kaluskar ,&nbsp;Chandrakant Singh ,&nbsp;Rajiv Kumar ,&nbsp;S.B. Chaudhari","doi":"10.1016/j.bcab.2025.103715","DOIUrl":"10.1016/j.bcab.2025.103715","url":null,"abstract":"<div><div><em>Uraria picta (Jacq.)</em> is also called Prishnaparni derived from Sanskrit, is a strong healing plant utilized in Ayurvedic and traditional Chinese medicine. While seeds can propagate it, the success rate is only up to 46 %, and seedlings are susceptible to damping-off disease. These seeds' small size and hard coating contribute to their low viability. This research intended to develop a frequent <em>in vitro</em> regeneration method for <em>U. picta</em> by investigating how different growth regulators impact explant establishment and growth. Shoot and nodal segments from <em>in vitro</em>-germinated <em>Uraria picta</em> plants were used, along with various combinations of 2,4-D, BA, and IAA. The addition of 2 mg L⁻¹ 2,4-D to MS media proved to be the most successful method for inducing and increasing callus formation from shoot and node explants during indirect organogenesis. The successful multiple shoot formation was achieved by culturing 2–3 cm nodes from <em>in vitro</em> grown plants on MS medium containing 1 mg L⁻¹ of BA in order to produce numerous shoots (22.00 shoots). This medium formulation resulted in the fastest shoot bud initiation (5.50 days) and the highest shoot frequency (99.0 %). MS media without hormones was the most effective for rooting, resulting in a rooting time of 6.87 days and a rooting frequency of 82.5 %. Following completion of the rooting process, the newly grown plants were subjected to a hardening phase, and the use of soil: cocopeat (1:1) and vermiculite: Perlite (2:1) blends resulted in a 90 % survival rate for <em>U. picta</em> plants.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103715"},"PeriodicalIF":3.8,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144852666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HPLC-DAD-ESI-MS/MS analysis of ethanolic extracts from date palm organs and investigation of pollen activity against reprotoxicity in male mice","authors":"Faïza Masmoudi ,&nbsp;Ahlem Ben Slima ,&nbsp;Walid Kriaa ,&nbsp;Mouna Chakroun ,&nbsp;Noureddine Drira ,&nbsp;Thomas Michel ,&nbsp;Xavier Fernandez ,&nbsp;Noureddine Allouche ,&nbsp;Bassem Khemakhem","doi":"10.1016/j.bcab.2025.103722","DOIUrl":"10.1016/j.bcab.2025.103722","url":null,"abstract":"<div><div>In dry and semi-arid areas of the world, date palm is a very significant plant. Date palm pollen possesses diverse health-promoting properties, particularly in enhancing male fertility. The chemical composition of different date palm organs as well as its pollen therapeutic property was examined in this study. Ethanolic extracts of pollen, male and female flowers, and fruits were investigated. Total phenolic, flavonoid and tannin contents were determined for all organs. The highest total phenolic and flavonoid contents were significantly (p &lt; 0.05) obtained in pollen extract. Twenty metabolites were identified using HPLC-DAD-ESI-MS/MS analysis. Besides two fatty acids, one lipid and five phenolic acids, twelve flavonoids were identified, including derivatives of quercetin, luteolin and isorhamnetin. The initial findings showed that concurrent dextamethrin treatment significantly (P &lt; 0.01) reduced accessory weights (sex organ weights, sperm count, and motility) as well as body and testicular weights. Histological examination of the seminiferous tubule lumen of mice intoxicated with deltaethrin showed significant morphological changes, including tubular necrosis and a low number of gametes. Furthermore, phytotherapeutic potential study of date palm pollen at dose of 20 mg/kg against reprotoxicity, induced by deltamethrin in male mice, was investigated. Interestingly, it was noted that spermatozoa motility and count were significantly (P &lt; 0.01) restored to the control group after pretreatment with date palm pollen extract. Additionally, administering date palm pollen extract raised SOD, CAT, and GSH activities in the testis considerably (P &lt; 0.01) and decreased the elevated level of MDA. Hence, date palm pollen can be considered as an interesting source of natural compounds useful for infertility treatment.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103722"},"PeriodicalIF":3.8,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144827719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic CCD-ANN optimization of organosolv–DES biphasic delignification for high-yield lignin isolation from banana inflorescence bract","authors":"Sundaram Deepika Bharathi,&nbsp;Samuel Jacob","doi":"10.1016/j.bcab.2025.103716","DOIUrl":"10.1016/j.bcab.2025.103716","url":null,"abstract":"<div><div>The efficient fractionation of lignocellulosic biomass into its primary constituents (cellulose, hemicellulose, and lignin) is pivotal for the advancement of sustainable biorefineries. In this study, banana inflorescence bract (BIB), an underutilized agro-waste was explored as a potential feedstock through an integrated pretreatment strategy. The process combined organosolv pretreatment with a subsequent deep eutectic solvent (DES)-based post-delignification step to enhance delignification and improve cellulose accessibility. Initially, the organosolv process parameters were optimized using a one-variable-at-a-time (OVAT) approach and further refined through central composite design (CCD)-based response surface methodology (RSM) and an artificial neural network integrated with a genetic algorithm (ANN-GA). The optimized ANN-GA model predicted and achieved a delignification of 64.77 ± 0.32 % (w/w) under the conditions of 1:12 (w/v) solid-to-liquid ratio at 75 °C for 120 min. To recover residual lignin, DES-based post-delignification treatment was conducted. The lignin fractions extracted from both organosolv and DES liquors were characterized using FT-IR, ¹H NMR spectroscopy, particle size analysis, and antioxidant activity assays. Furthermore, enzymatic saccharification of the pretreated biomass yielded 41.95 ± 0.10 % (w/w) and 57.97 ± 0.21 % (w/w) of fermentable sugars from organosolv-treated and DES-treated solid residues respectively, indicating improved cellulose digestibility. In addition, the solvents used in this study were recycled and reused for three successive treatment cycles, achieving a recovery of 85 % for ethanol and 83.33 % for DES that could enhance the environmental and economic feasibility of the process. Hence, this study underscores the potential of BIB as a viable, sustainable feedstock for biocatalytic applications and lignin valorization in modern biorefineries.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103716"},"PeriodicalIF":3.8,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144840871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic Silver nanoparticles from Thottea siliquosa: Synthesis, characterization, and evaluation of antioxidant, anti-inflammatory, anticancer, and antibacterial activities","authors":"Aneeta Renny ,&nbsp;Alby Tom ,&nbsp;Aparna Prasad ,&nbsp;Aswathi Moothakoottil Kuttithodi ,&nbsp;Pooja Mohan Padmalayam ,&nbsp;Satheesh George ,&nbsp;Shaji E. Mathew ,&nbsp;Joice Tom Job ,&nbsp;Arunaksharan Narayanankutty","doi":"10.1016/j.bcab.2025.103710","DOIUrl":"10.1016/j.bcab.2025.103710","url":null,"abstract":"<div><div>Traditional herbal medicines are important sources of drug candidates against various diseases. Though the plant-derived molecules are active secondary metabolites with potential pharmacological efficacy, their utility is largely limited due to reduced bioavailability and half-life. Conversion to nano-formulation and biogenic nanoparticles is considered to be an alternative for overcoming the issues associated with herbal medicines. In the present study, green synthesized nanoparticles from the methanolic leaf extract of <em>Thottea siliquosa,</em> a traditionally used ayurvedic plant belonging to the family Aristolochiaceae, were assessed for their biological efficacy. The characterization of silver nanoparticles from <em>Thottea siliquosa</em> (TNP) was carried out using X-ray Diffraction and Transmission Electron Microscopy, where the size of the particle was estimated to be between 15 and 34 nm (29.95 nm as per XRD). TNP showed significant radical scavenging capabilities as indicated by DPPH (132.7 ± 3.7 μg/mL), ABTS (106.0 ± 16.33 μg/mL), and peroxide radical scavenging (116.27 ± 4.1 μg/mL). In addition, the TNP also showed significant anti-inflammatory activity in Raw 264.7 macrophages triggered by Lipopolysaccharide (LPS) by inhibiting the release of inflammatory cytokines. The anticancer activity of TNP was also assessed against MCF7 (3.02 ± 0.19 μg/mL), MDA-MB-231 (5.41 ± 0.22 μg/mL), and HCT15 cells (6.07 ± 0.11 μg/mL). Overall, the study revealed that the TNP exhibits significant antioxidant, anti-inflammatory, anticancer, and antibacterial activities.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103710"},"PeriodicalIF":3.8,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144827622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the potential of laccase from Grammothele sp. (pp464104.1) for polystyrene biodegradation: A promising approach for plastic waste management","authors":"Karthikeyan Ramamurthy ,&nbsp;Meenatchi Ramu ,&nbsp;N. Paul Thomas ,&nbsp;Ashraf Atef Hatamleh ,&nbsp;Bassam Khalid Alnafisi ,&nbsp;Sungkwon Park ,&nbsp;S. Karthick Raja Namasivayam ,&nbsp;Jesu Arockiaraj","doi":"10.1016/j.bcab.2025.103714","DOIUrl":"10.1016/j.bcab.2025.103714","url":null,"abstract":"<div><div>Laccase (EC 1.10.3.2) is a multicopper oxidase enzyme found in plants, fungi, and bacteria. It oxidizes various phenolic substrates through one-electron oxidations, causing cross-linking. Laccase can degrade polymers like lignin, a complex natural polymer of phenylpropanoid units linked by ether and carbon-carbon bonds. It also degrades polystyrene, a synthetic polymer derived from petroleum, composed of repeating styrene monomers that have a linear hydrocarbon backbone with phenyl groups. This study focuses on the laccase enzyme derived from <em>Grammothele</em> sp., a fungus recognized for its significant ligninolytic activity, and evaluates its potential in environmental biotechnology. The fungal strain was isolated and identified through ITS region phylogenetic analysis, confirming it as <em>Grammothele</em> sp. The laccase produced by the fungus was purified using Sephadex G-100 column chromatography, yielding a molecular weight of approximately 63 kDa as determined by SDS-PAGE. The enzyme exhibited optimal activity at 27 °C and pH 6.0, with notable plastic degradation observed under these conditions. Inhibition studies indicated sensitivity to specific inhibitors and metal ions, particularly Cu²⁺, Fe²⁺, Mn²⁺, and Cd²⁺, which reduced laccase activity. Importantly, the purified laccase demonstrated significant degradation of polystyrene, as confirmed by FTIR and SEM analyses, which revealed substantial chemical transformations and physical erosion of polystyrene particles. These findings highlight the enzyme's potential for mitigating plastic pollution. However, challenges such as enzyme inhibition and sensitivity to metal ions must be addressed. In conclusion, laccase from Grammothele sp. shows considerable promise for biotechnological applications in lignin degradation and plastic biodegradation, with future research aimed at optimizing its use in various environmental contexts.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103714"},"PeriodicalIF":3.8,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144840872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of honey-enriched soursop leaves (Annona muricata) kombucha on lipid profiles and hypoglycemic properties: An in-vivo study","authors":"Andy Candra ,&nbsp;Haile Fentahun Darge","doi":"10.1016/j.bcab.2025.103713","DOIUrl":"10.1016/j.bcab.2025.103713","url":null,"abstract":"<div><div>This study investigated the hypoglycemic and lipid-modulating effects of honey-enriched soursop (<em>Annona muricata</em>) leaves kombucha in alloxan-induced diabetic Wistar rats. Diabetes was induced using alloxan monohydrate (45 mg/kg BW), followed by 28 days of oral administration of kombucha at 0.5 %, 1.0 %, or 1.5 % (w/v; 5 mL/kg BW/day), metformin (45 mg/kg BW/day), or no treatment. The 1.5 % kombucha dose markedly reduced blood glucose from 224.4 ± 4.77 to 99.4 ± 7.82 mg/dL, comparable to metformin (107.4 ± 7.43 mg/dL). It also improved lipid profiles, lowering total cholesterol (59.0 ± 5.33 mg/dL), triglycerides (34.6 ± 8.32 mg/dL), and LDL (10.0 ± 7.10 mg/dL), while elevating HDL (47.6 ± 7.76 mg/dL). Histological examination revealed pancreatic islet regeneration in treated groups. <em>In vivo</em> toxicity evaluation using <em>Zophobas morio</em> larvae showed no adverse effects. These results demonstrate that honey-enriched soursop leaves kombucha is a safe and effective natural candidate for managing diabetes and dyslipidemia.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103713"},"PeriodicalIF":3.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-engineered silver nanoparticles suppress flic-mediated motility and induce argI-driven metabolic rewiring in Escherichia coli","authors":"Moumita Sil ,&nbsp;Anamitra Goswami ,&nbsp;Igor Polikarpov ,&nbsp;Arunava Goswami","doi":"10.1016/j.bcab.2025.103712","DOIUrl":"10.1016/j.bcab.2025.103712","url":null,"abstract":"<div><div>The rise of antimicrobial resistance necessitates novel treatment approaches, with bio-synthesized nanoparticles offering promising alternatives. This study explores silver nanoparticles (bio-AgNPs) synthesized using Terminalia chebula extract, characterized via spectroscopy and microscopy techniques. Bio-AgNPs exhibited a surface plasmon resonance peak at 466 nm, an average hydrodynamic diameter of 150.3 nm, and a zeta potential of −41.55 mV, ensuring colloidal stability. Antibacterial efficacy was evaluated against <em>Escherichia coli</em> K12, with a minimum inhibitory concentration of 78.125 ppm. Transcriptomic profiling revealed a significant downregulation of flagellar biosynthesis and assembly genes—including fliC (encoding flagellin, the main structural protein of bacterial flagella), flgB, flgC, flgD, flgE, fliH, flgG, fliI, flgH, fliJ, and fliK—impairing bacterial chemotaxis and reducing motility. Concurrently, suppression of the citT gene disrupted citrate import via the citrate-succinate antiporter, impairing TCA cycle flux and limiting carbon metabolism. Conversely, genes involved in L-arginine biosynthesis and transport—including argI (encoding ornithine carbamoyltransferase, a key enzyme in the arginine biosynthesis pathway), argC, carA, argD, argA, argG, artP, artQ, artJ, and artI—were upregulated, indicating a metabolic shift toward arginine catabolism via the arginine deiminase pathway under oxidative stress. Arginine is known to inhibit Na⁺-Na-dependent flagellar motors by competitively interfering with sodium binding, further exacerbating motility impairment. The dual antimicrobial action—disrupting flagellar function and inducing metabolic stress—suggests a potent biochemical mechanism to counteract bacterial adaptation and resistance.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103712"},"PeriodicalIF":3.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}