Biocatalysis and agricultural biotechnology最新文献_第7页

A polysaccharide from Schizophyllum commune enhances tomato resistance to TYLCV by modulating photosynthetic performance and antioxidant defense 裂叶属植物的多糖通过调节光合性能和抗氧化防御增强番茄对TYLCV的抗性

IF 4.6

Biocatalysis and agricultural biotechnology Pub Date : 2026-08-01 Epub Date: 2026-08-12 DOI: 10.1016/j.bcab.2026.104220

Haiwen Dong, Wei Liu, Hetong Yang, Vladimir A. Vinokurov, Meimei Wu, Shuhao Huo

{"title":"A polysaccharide from Schizophyllum commune enhances tomato resistance to TYLCV by modulating photosynthetic performance and antioxidant defense","authors":"Haiwen Dong, Wei Liu, Hetong Yang, Vladimir A. Vinokurov, Meimei Wu, Shuhao Huo","doi":"10.1016/j.bcab.2026.104220","DOIUrl":"10.1016/j.bcab.2026.104220","url":null,"abstract":"<div><div>Functional polysaccharides have gained increasing attention as promising biocontrol agents for the sustainable control of crop viral diseases. <em>Schizophyllum commune</em> is an edible and medicinal fungus rich in bioactive polysaccharides; however, its potential in plant antiviral defense remains unexplored. In this study, a polysaccharide (SCPS) was isolated and purified from <em>Schizophyllum commune</em>, and its antiviral potential against TYLCV was investigated. Analysis revealed that SCPS is a slightly acidic polysaccharide composed primarily of glucose, mannose, and galactose, with a typical β-type pyranose configuration. Pot experiments showed that foliar application of SCPS at 50 mg/L significantly enhanced tomato resistance against TYLCV, achieving prophylactic and curative efficacies of 38.30% and 44.13%, respectively, which were superior to those of the commercial agent Lentinan. Mechanistic studies revealed that SCPS exerted a dual modes of action: (i) it could directly bind to TYLCV particles, reducing the free virus content; (ii) it enhanced defense-related physiological responses by maintaining PSII stability, improving electron transport efficiency, and strengthening antioxidant defense capacity, as indicated by increased SOD and POD activities by 62.7% and 45.3%, respectively. In summary, SCPS, as a fungal polysaccharide-based bioprotectant, possesses both antiviral activity and the capacity to enhance defense-related physiological defense responses, thereby offering a potential strategy for the development of environmentally friendly agents for sustainable crop protection.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"76 ","pages":"Article 104220"},"PeriodicalIF":4.6,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148731751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential utilization of invasive weed Ageratum conyzoides in PVA/sodium alginate/chitosan hydrogel as a delivery medium for natural antibacterial agents PVA/海藻酸钠/壳聚糖水凝胶作为天然抗菌剂递送介质的应用前景

IF 4.6

Biocatalysis and agricultural biotechnology Pub Date : 2026-08-01 Epub Date: 2026-08-20 DOI: 10.1016/j.bcab.2026.104228

Nabila Asy Syifa, Kusjuriansah Kusjuriansah, Halida Rahmi Luthfianti, William Xaveriano Waresindo, Dian Ahmad Hapidin, Tri Suciati, Dhewa Edikresnha, Khairurrijal Khairurrijal

{"title":"Potential utilization of invasive weed Ageratum conyzoides in PVA/sodium alginate/chitosan hydrogel as a delivery medium for natural antibacterial agents","authors":"Nabila Asy Syifa, Kusjuriansah Kusjuriansah, Halida Rahmi Luthfianti, William Xaveriano Waresindo, Dian Ahmad Hapidin, Tri Suciati, Dhewa Edikresnha, Khairurrijal Khairurrijal","doi":"10.1016/j.bcab.2026.104228","DOIUrl":"10.1016/j.bcab.2026.104228","url":null,"abstract":"<div><div>Invasive weed, such as <em>Ageratum conyzoides</em>, pose not only ecological challenges but also have potential as a value-added bioresource. This study utilized an environmentally friendly repeated freeze-thaw method to utilize and develop <em>A.conyzoides</em> leaf extract (ACLE) in PVA/SA/CS hydrogel, thereby enhancing its antibacterial activity. LC-HRMS and GC-MS analysis identified flavonoids, alkaloids, terpenoids, and other related compounds as the main contributors to the activity. SEM characterization revealed a porous structure with a loose physical cross-linking network, resulting in a high swelling capacity (266.53 ± 0.59% w/w). The integration of ACLE in the hydrogel matrix was confirmed through FTIR spectra. Mechanical tests showed that the compressive modulus of the hydrogel ranged from 0.152 ± 0.004 MPa – 0.193 ± 0.026 MPa, close to the value of commercial wound dressing Hydrosorb® (∼0.2 MPa) and within the range of Young's modulus of human skin (25 kPa–7.8 MPa), making it suitable for topical application without causing excessive stress. Although its antibacterial activity against <em>Staphylococcus aureus</em> and <em>Pseudomonas aeruginosa</em> has not yet matched that of broad-spectrum antibiotics, the highest antibacterial value of 68.05 ± 0.37% still shows significant potential, especially against <em>S.aureus</em>. Cytotoxicity tests against normal cells yielded an IC50 value greater than 500 μg/mL, indicating good biocompatibility. The release profile of ACLE (16.18% – 30.75% w/w) over 3 h exhibited a time-dependent, slow diffusion pattern, suitable for prolonged delivery applications. These results confirm the potential of utilizing invasive weed biomass to produce antibacterial biomaterials, supporting the development of future weed control strategies.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"76 ","pages":"Article 104228"},"PeriodicalIF":4.6,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148854132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mixed-strain fermentation enhances protein content, phenolic composition, antioxidant capacity, and free amino acid content in brewer's spent grain 混合菌种发酵提高了啤酒废粮的蛋白质含量、酚类成分、抗氧化能力和游离氨基酸含量

IF 4.6

Biocatalysis and agricultural biotechnology Pub Date : 2026-08-01 Epub Date: 2026-07-14 DOI: 10.1016/j.bcab.2026.104178

Lucas Correia Silva, Izabelle Alves de Castro França, Larine Kupski, Beatriz Cervejeira Bolanho Barros, Grasiele Scaramal Madrona

{"title":"Mixed-strain fermentation enhances protein content, phenolic composition, antioxidant capacity, and free amino acid content in brewer's spent grain","authors":"Lucas Correia Silva, Izabelle Alves de Castro França, Larine Kupski, Beatriz Cervejeira Bolanho Barros, Grasiele Scaramal Madrona","doi":"10.1016/j.bcab.2026.104178","DOIUrl":"10.1016/j.bcab.2026.104178","url":null,"abstract":"<div><div>This study evaluated the potential of simultaneous solid-state fermentation (SSF) with <em>Rhizopus oryzae</em> and <em>Trichoderma virens</em> in enhancing the nutritional quality of brewer's spent grain (BSG). The effects of fungal ratio (1 × 10<sup>8</sup> spores of <em>R. oryzae</em> to 1 × 10<sup>8</sup> spores of <em>T. virens)</em>, moisture content (27.0 – 73.0%), temperature (25 – 40 °C), and fermentation time (0 – 96 h) were investigated. Mixed-strain fermentation (1:1 fungal ratio) of BSG at 73.0% moisture and 28.7 °C resulted in the higher protein content (23.60 g 100 g<sup>−1</sup>), than that present in unfermented BSG (20.82 g 100 g<sup>−1</sup>). Under these conditions, antioxidant activity (AA) increased by 376.0% to 625.0%, as assessed by free radical scavenging methods and the ferric reducing antioxidant power assay. Enzyme activities increased during SSF (0 to 96 h), thereby modulating the phenolic profile of BSG. The contents of ferulic and vanillic acids were associated with total cellulase activity, whereas isovanillin and syringaldehyde contents were correlated with amylase, endoglucanase, and protease activities. The release of these phenolic compounds explained the increase in AA, with the highest values being observed after 72 h of mixed-strain fermentation. The free amino acid levels of BSG increased by 6.1 to 16.4 times after SSF. This study showed that mixed-strain fermentation using <em>R. oryzae</em> and <em>T. virens</em> is a viable alternative to enhance the nutritional value of BSG, yielding a value-added ingredient suitable for food formulations.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"76 ","pages":"Article 104178"},"PeriodicalIF":4.6,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148651040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production and characterization of porous starch from foxtail millet (Setaria italica L.) using α-amylase, glucoamylase, and mixed enzymes α-淀粉酶、葡萄糖淀粉酶和混合酶制备谷子多孔淀粉的研究

IF 4.6

Biocatalysis and agricultural biotechnology Pub Date : 2026-08-01 Epub Date: 2026-07-21 DOI: 10.1016/j.bcab.2026.104190

Rifqah Amaliah Syam, Djagal Wiseso Marseno, Beom Soo Kim, Andri Frediansyah, Lucia Dhiantika Witasari

{"title":"Production and characterization of porous starch from foxtail millet (Setaria italica L.) using α-amylase, glucoamylase, and mixed enzymes","authors":"Rifqah Amaliah Syam, Djagal Wiseso Marseno, Beom Soo Kim, Andri Frediansyah, Lucia Dhiantika Witasari","doi":"10.1016/j.bcab.2026.104190","DOIUrl":"10.1016/j.bcab.2026.104190","url":null,"abstract":"<div><div><em>Setaria italica</em> L. starch requires modification to enhance its functional properties and broaden its industrial applications, for instance, by modifying it into porous starch (PS). This study aimed to generate PS from foxtail millet through enzymatic hydrolysis employing α-amylase, glucoamylase, and their combinations (mixed enzymes) at various concentrations, followed by characterization of its physical, chemical, and functional properties. The starch hydrolyzed by mixed enzymes showed the highest solubility, reaching 76.13%. The highest oil absorption capacity (OAC) of 172.98% was obtained from hydrolysis using α-amylase (100 U/g), while the highest water absorption capacity (WAC) of 97.74% was achieved with glucoamylase at 25 U/g. Moreover, the maximum adsorption capacity of 80.45% was obtained from the starch hydrolyzed by mixed enzymes at a concentration ratio of 50:25 U/g. Based on these findings, porous starch derived from <em>Setaria italica</em> L. demonstrated promising adsorption and absorption properties, suggesting potential for future development as an adsorbent or encapsulation carrier, although application-specific validation remains necessary.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"76 ","pages":"Article 104190"},"PeriodicalIF":4.6,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148637572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering the role of potato peel on sunflower hybrids growth, photosynthesis, osmoregulation, oxidative defense and ion uptake under salinity 盐胁迫下马铃薯皮对向日葵杂种生长、光合作用、渗透调节、氧化防御和离子吸收的影响

IF 4.6

Biocatalysis and agricultural biotechnology Pub Date : 2026-08-01 Epub Date: 2026-08-25 DOI: 10.1016/j.bcab.2026.104233

Sundas Fatima, Aasma Tufail, Muhammad Bilal Hafeez, Samreen Nazeer, Noreen Zahra, Fatima Latif, Sabina Asghar, Muhammad Ahsan, Muhammad Hamza, Muhammad Zubair Akram

{"title":"Deciphering the role of potato peel on sunflower hybrids growth, photosynthesis, osmoregulation, oxidative defense and ion uptake under salinity","authors":"Sundas Fatima, Aasma Tufail, Muhammad Bilal Hafeez, Samreen Nazeer, Noreen Zahra, Fatima Latif, Sabina Asghar, Muhammad Ahsan, Muhammad Hamza, Muhammad Zubair Akram","doi":"10.1016/j.bcab.2026.104233","DOIUrl":"10.1016/j.bcab.2026.104233","url":null,"abstract":"<div><div>Potato peel contains significant levels of bioactive compounds, especially phenolics and antioxidants, which may contribute to mitigating abiotic stress effects when applied as plant-derived biostimulants. However, the influence of potato peel on photosynthetic pigments, antioxidant machinery, reactive oxygen species metabolism and ion homeostasis has yet to be explored in sunflower hybrids (FH-741, FH-780, FH-793, FH-800, FH-802) under salinity stress (150 mM). In the present study, salinity notably decreased growth, relative water contents, photosynthetic pigments, and mineral acquisition in all hybrids. However, sunflower hybrids subjected to sodium toxicity showed excessive sodium accumulation. Salinity produced substantial oxidative stress (malondialdehyde and hydrogen peroxide) in all sunflower hybrids but also marginally enhanced the antioxidants and osmolytes in them. However, potato peel application decreased the toxic influence of salinity by improving the antioxidants and osmolytes levels which lessen the oxidative damage in all sunflower hybrids. Under salt stress, it also improved the relative water content, photosynthetic pigments and mineral acquisition which ultimately better growth parameters of all hybrids, especially the FH-780, and FH-800. Thus, the potato peel enabled the sunflower hybrids to withstand salinity condition by regulating physiological and biochemical functions. In agriculture, it has great potential to mitigate the adverse effects of salinity on sunflower through potato peel application.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"76 ","pages":"Article 104233"},"PeriodicalIF":4.6,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148854110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic biodegradation of polyethylene terephthalate microplastics and polyhydroxybutyrate coproduction by Arthrospira platensis under photo-Fenton stress 光fenton胁迫下平节螺旋藻协同降解聚对苯二甲酸乙二醇酯微塑料和聚羟基丁酸酯

IF 4.6

Biocatalysis and agricultural biotechnology Pub Date : 2026-08-01 Epub Date: 2026-08-15 DOI: 10.1016/j.bcab.2026.104225

Muhamad Maulana Azimatun Nur, Dyah Ayu Musyrifah, Erica Munazah

{"title":"Synergistic biodegradation of polyethylene terephthalate microplastics and polyhydroxybutyrate coproduction by Arthrospira platensis under photo-Fenton stress","authors":"Muhamad Maulana Azimatun Nur, Dyah Ayu Musyrifah, Erica Munazah","doi":"10.1016/j.bcab.2026.104225","DOIUrl":"10.1016/j.bcab.2026.104225","url":null,"abstract":"<div><div>The accumulation of polyethylene terephthalate (PET) microplastics necessitates advanced remediation strategies that transcend conventional kinetic limitations. This study evaluated a synergistic photo-oxidative and biological system utilizing <em>Arthrospira platensis</em> and <em>Skeletonema costatum</em> for simultaneous PET degradation and biopolymer coproduction. Initial screening revealed <em>A. platensis</em> achieved a four-fold higher degradation rate than <em>S. costatum</em>. Subsequent optimization of co-metabolic interactions (15 mg/L Fe-EDTA, 500 mg/L glycerol, 350 mW/m<sup>2</sup> UV-C) maximized the degradation process, achieving a 0.92%/d rate and 66.11% surface erosion. FTIR spectroscopy definitively confirmed true chemical depolymerization, revealing extensive polymer chain scission via the systematic attenuation of ester carbonyl and C-O-C linkages. Oxidative stress conditions redirected the microalgal metabolic flux toward energy storage, accumulating high-purity polyhydroxybutyrate (PHB) up to 26.05 mg/L. This research established a viable closed-loop biorefinery approach, effectively upcycling recalcitrant plastic waste into value-added biopolymers through stress-induced mixotrophic metabolism.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"76 ","pages":"Article 104225"},"PeriodicalIF":4.6,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148770677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmentally conscious extraction of chitin and calcium acetate from Portunus segnis shell waste via RSM-CCD optimized acetic acid demineralization 基于RSM-CCD优化的醋酸脱矿法提取海螺壳废弃物中的几丁质和乙酸钙

IF 4.6

Biocatalysis and agricultural biotechnology Pub Date : 2026-08-01 Epub Date: 2026-07-18 DOI: 10.1016/j.bcab.2026.104186

Raghda Yazidi, Majdi Hammami, Soumaya Sayeb, Walid Yeddes, Hamza Ghadoumi, Mounir Ferhi, Wissem Aidi Wannes, Soumaya Bourgou, Mouna Ben Farhat, Karima Horchani-Naifer, Moufida Saidani Tounsi

{"title":"Environmentally conscious extraction of chitin and calcium acetate from Portunus segnis shell waste via RSM-CCD optimized acetic acid demineralization","authors":"Raghda Yazidi, Majdi Hammami, Soumaya Sayeb, Walid Yeddes, Hamza Ghadoumi, Mounir Ferhi, Wissem Aidi Wannes, Soumaya Bourgou, Mouna Ben Farhat, Karima Horchani-Naifer, Moufida Saidani Tounsi","doi":"10.1016/j.bcab.2026.104186","DOIUrl":"10.1016/j.bcab.2026.104186","url":null,"abstract":"<div><div>This study presented an environmentally conscious approach for the simultaneous extraction of chitin and calcium acetate from shells of the invasive Atlantic blue crab (<em>Portunus segnis</em>), an abundant marine waste in the Mediterranean Sea. The demineralization process was optimized using Response Surface Methodology (Central Composite Design) to investigate the effects of acetic acid concentration, reaction time, and solid-to-liquid ratio on residual ash content, final pH, and dry matter yield. Under optimized conditions, the demineralized chitin exhibited very low ash content (∼0.10%), confirming efficient mineral removal. The extracted chitin was obtained with a degree of acetylation of 83% and was characterized as α-chitin by FTIR (characteristic amide bands at 1622 and 1554 cm<sup>−1</sup>), XRD (prominent peak at 2θ ≈ 19.8°, CrI ≈ 34%), and SEM (porous fibrillar microstructure). It displayed high water holding capacity (785%) and oil holding capacity (542%), indicating strong potential for functional applications. Calcium acetate monohydrate recovered from the filtrate was confirmed by FTIR and XRD, with an experimental yield of 42.56%. This dual-valorization strategy not only minimizes environmental impact compared to traditional strong acid methods but also introduces a zero-waste circular bioeconomy model by converting invasive species waste into two valuable bioproducts, demineralized chitin and marketable calcium acetate, in a single integrated process.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"76 ","pages":"Article 104186"},"PeriodicalIF":4.6,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148651158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges of using ensiled sorghum in anaerobic co-digestion with beef cattle manure: Effects on process stability and nutrient recovery 青贮高粱与肉牛粪便厌氧共消化的挑战：对工艺稳定性和养分回收的影响

IF 4.6

Biocatalysis and agricultural biotechnology Pub Date : 2026-08-01 Epub Date: 2026-07-23 DOI: 10.1016/j.bcab.2026.104194

Eduardo Luiz Buligon, Jéssica Caroline de Lima, Ritieli Marostica, Jorge de Lucas Junior, Monica Sarolli Silva de Mendonça Costa

{"title":"Challenges of using ensiled sorghum in anaerobic co-digestion with beef cattle manure: Effects on process stability and nutrient recovery","authors":"Eduardo Luiz Buligon, Jéssica Caroline de Lima, Ritieli Marostica, Jorge de Lucas Junior, Monica Sarolli Silva de Mendonça Costa","doi":"10.1016/j.bcab.2026.104194","DOIUrl":"10.1016/j.bcab.2026.104194","url":null,"abstract":"<div><div>This study evaluated the performance of anaerobic co-digestion (ACoD) of manure from confined beef cattle with Bolivian giant sorghum under batch conditions. Sorghum was tested in fresh and ensiled forms, with and without panicles, to assess process stability, biogas and methane production, and the agronomic quality of the resulting digestate. Treatments with fresh sorghum showed performance similar to that of anaerobic mono-digestion of manure, with stable operation and high methane yields. In contrast, ensiled sorghum led to process instability, characterised by volatile fatty acid accumulation, VA/TA ratios above 2.0, and reduced methane production. The treatment with ensiled sorghum, including panicles, exhibited the lowest stability and produced digestate with reduced nitrogen, phosphorus, and potassium contents. These results demonstrate that, although ensiling ensures biomass preservation, it can negatively affect process stability and nutrient recovery in batch ACoD systems. Adjustments to substrate proportions or process configurations are required to enable the efficient use of ensiled energy crops.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"76 ","pages":"Article 104194"},"PeriodicalIF":4.6,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148651195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical characterization and cultivation optimization of the red microalga Porphyridium sp. Näg. P-519 for exopolysaccharide production 红微藻卟啉藻（Porphyridium sp. Näg）的生化特性及培养优化。P-519用于胞外多糖的生产

IF 4.6

Biocatalysis and agricultural biotechnology Pub Date : 2026-08-01 Epub Date: 2026-07-17 DOI: 10.1016/j.bcab.2026.104185

Olga Babich, Svetlana Ivanova, Ekaterina Budenkova, Egor Kashirskikh, Anastasia Volkova, Yulia-Danaya Boichenko, Marina Krasnovskikh, Stanislav Sukhikh

{"title":"Biochemical characterization and cultivation optimization of the red microalga Porphyridium sp. Näg. P-519 for exopolysaccharide production","authors":"Olga Babich, Svetlana Ivanova, Ekaterina Budenkova, Egor Kashirskikh, Anastasia Volkova, Yulia-Danaya Boichenko, Marina Krasnovskikh, Stanislav Sukhikh","doi":"10.1016/j.bcab.2026.104185","DOIUrl":"10.1016/j.bcab.2026.104185","url":null,"abstract":"<div><div>The cultivation conditions for the microalga <em>Porphyridium</em> sp. Näg. strain P-519 from the collection of the Institute of Physiological Research (RAS, Russia) were enhanced in the course of the study. The scientific novelty lies in the presentation of the biochemical characteristics of this strain and the chemical conditions of its cultivation that influence the strain's productivity and promote maximum exopolysaccharide synthesis. The optimal temperature and pH ranges for cultivation were 25 ± 1 °C and 6-8, respectively. This microalga is a producer of total extracellular carbohydrates: maximum 67.8 mg/mL (66% of total cell suspension with media dry weight) when isolated by ethanol precipitation. The most efficient solvents for pigment extraction are extraction buffer and methanol. The predominant pigment in the cell is allophycocyanin, with a content of 4.908 ± 0.075 mg/g dry weight when extracted in citrate buffer. HPLC analysis preliminarily characterized the exopolysaccharides' monosaccharide composition as rhamnose (31%), fructose (30%), fucose (18%), galactose (15%), and sucrose (6%). When different media were used, cell morphology of <em>Porphyridium</em> sp. Näg. changed: on media with artificial seawater, the cell pigment is red, on Brody & Emerson Max, the cell pigment is pink. Tap water induced cell distress and subsequent death, which was associated with an elevated level of total extracellular carbohydrates. Distinguishing between stress-induced extracellular carbohydrates production and cell death requires additional cell viability assays. This study reveals the biochemical potential of the red microalgae <em>Porphyridium</em> sp. Näg. and provides a basis for intensification of its cultivation by selecting an optimal culture medium.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"76 ","pages":"Article 104185"},"PeriodicalIF":4.6,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148637117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integration of taro peel valorization and starch affinity purification for the production of a thermostable alpha-amylase from Bacillus paralicheniformis RAM9 副青衣芽孢杆菌RAM9中芋头皮增值和淀粉亲和纯化的结合制备耐热α -淀粉酶

IF 4.6

Biocatalysis and agricultural biotechnology Pub Date : 2026-08-01 Epub Date: 2026-08-03 DOI: 10.1016/j.bcab.2026.104206

Maha M. El-Attar, Amira M. Embaby, Hesham M. Saeed, Hoda E. Mahmoud, Amira A. Matrawy

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