Biocatalysis and agricultural biotechnology最新文献

{"title":"Immobilization strategies for alkaline protease: A comprehensive overview from fundamental knowledge to recent advances in techniques and applications","authors":"Mazen Khaled Alsahari ,&nbsp;Sami Karam ,&nbsp;Hani Abdulwahid Alhajj ,&nbsp;Yan Luo ,&nbsp;Feng F. Hong","doi":"10.1016/j.bcab.2025.103900","DOIUrl":"10.1016/j.bcab.2025.103900","url":null,"abstract":"<div><div>Alkaline proteases are essential biocatalysts with broad industrial relevance due to their high activity and stability under alkaline and high-temperature conditions. Recent studies have shown that immobilized alkaline proteases can keep more than 80 % of their original activity after 8–10 reuse cycles. When conditions are right, they can be four times more efficient as catalysts than free forms. This shows that immobilization can improve operational performance. Moreover, advancements in carrier design have contributed to reducing immobilization costs by approximately 60 %, highlighting the growing economic feasibility of these systems. This review summarizes the recent progress in immobilization strategies for alkaline proteases over the past five years, with an emphasis on approaches that enhance enzyme stability, reusability, and catalytic efficiency. It compares four key techniques: covalent binding, adsorption, entrapment-encapsulation, and cross-linking, highlighting their benefits and limitations. Furthermore, it discusses recent innovations such as nanostructured hybrid carriers, magnetic metal-organic frameworks (MOFs), and multifunctional composites that minimize enzyme leakage and activity loss. Finally, the review evaluates industrial and biocatalytic applications, providing insights and statistical guidance for selecting cost-effective and eco-friendly immobilization methods. Consequently, this work serves as a valuable resource for researchers and practitioners seeking to optimize alkaline protease immobilization for enhanced performance in diverse applications.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"71 ","pages":"Article 103900"},"PeriodicalIF":3.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145838010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of waste-derived Tamarindus indica fruit (WD-TIF)-based polyphenol-encapsulated Ag-NPs: A next-generation sustainable antibiotic material","authors":"Gad Elsayed Mohamed Salem ,&nbsp;Mohamed A. El-Sakhawy ,&nbsp;Zahraa Zakarya Abd El-Hafez Mohammad ,&nbsp;Shaymaa A. Mohamed ,&nbsp;Neetu Talreja ,&nbsp;Wirongrong Tongdeesoontorn ,&nbsp;Mohammad Ashfaq","doi":"10.1016/j.bcab.2025.103908","DOIUrl":"10.1016/j.bcab.2025.103908","url":null,"abstract":"<div><div>The emergence of bacterial resistance has been incessantly increasing and is one of the most pressing global health threats today. Bacterial resistance is a “silent pandemic” that requires immediate, effective intervention rather than deferral to a future scenario. Therefore, the sustainable development of NPs might address issues associated with conventional synthesis while maintaining high biocompatibility. In this regard, the present study focuses on the synthesis of Ag-NPs from waste of <em>Tamarindus indica</em> fruit (WD-TIF-Ag) via a microwave process. The WD-TIF simultaneously acted as a reducing and encapsulating agent, thereby significantly improving the effectiveness of the WD-TIF-Ag composite due to its polyphenol content. The as-prepared WD-TIF-Ag composite was characterised using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and Fourier-transform infrared (FTIR) spectroscopy. The data confirms the successful synthesis of spherical Ag-NPs with a primary crystallite size of ∼12.5 nm and composite clusters in the 40–60 nm range. The as-prepared WD-TIF-Ag composite has effective antibacterial activity against both gram-negative (<em>Escherichia coli</em> (<em>E. coli</em>)) and gram-positive (<em>Staphylococcus aureus (S. aureus)</em>) bacterial strains. The MIC value (10 μg/mL) and MBC value (40 μg/mL) of the WD-TIF-Ag composite were observed against <em>S. aureus</em>. Therefore, the as-prepared WD-TIF-Ag developed in this study is a facile, cost-effective, and sustainable approach to the development of antibiotics.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"71 ","pages":"Article 103908"},"PeriodicalIF":3.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145838008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the advantages of Lactobacillus species from plants for future therapeutics and foods","authors":"Sureeporn Wichiansri ,&nbsp;Surasak Siripornadulsil ,&nbsp;Hironori Iwasaki ,&nbsp;Wilailak Siripornadulsil","doi":"10.1016/j.bcab.2025.103889","DOIUrl":"10.1016/j.bcab.2025.103889","url":null,"abstract":"<div><div>Lactic acid bacteria (LAB) are known for fermenting sugars into beneficial metabolites that promote host health, making them promising candidates for probiotic development. This study focused on isolating LAB from various plant sources, assessing their probiotic properties, evaluating safety features, and exploring their potential use in fruit-based probiotic products. Among 140 LAB isolates from various plant sources, 16 were classified into nine species, with 5 identified as potential probiotics: <em>Lacticaseibacillus casei</em>, <em>Lacticaseibacillus paracasei</em>, <em>Lactiplantibacillus plantarum</em>, <em>Lacticaseibacillus rhamnosus</em>, and <em>Lactococcus lactis</em>. These isolates, along with commercial probiotics, exhibited multidrug-resistance (MDR) to certain antibiotics, particularly from the aminoglycoside, glycopeptide, and polyketide classes. Unprotected LAB cells demonstrated resistance to simulated gastric juice at pH 2 and effectively inhibited both gram-positive and gram-negative bacterial pathogens. Notably, three strains isolated from fruits and flowers: <em>L. paracasei</em> OR1, <em>L. plantarum</em> FL10, and <em>L. rhamnosus</em> MA3, survived in low-pH orange and coconut juices during cold storage, with a significant increase in viable cell counts after 24 h, indicating their ability to ferment sugars in natural fruit juices. <em>L. paracasei</em> OR1, derived from oranges, exhibited the highest viability and an MDR profile comparable to commercial strains. Whole-genome analysis of OR1 revealed genes associated with bacteriocin production, amino acid biosynthesis, and antioxidant activity, with no genes related to acquired antibiotic resistance. These findings suggest that OR1 could be cultivated in high-fructose fruit juices to produce viable probiotic cells and bioactive agents, with therapeutic potential. However, additional assessment is needed to confirm safety and prevent resistance gene transfer.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"71 ","pages":"Article 103889"},"PeriodicalIF":3.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insecticidal activity of saline extract, protein fraction, and lectin (BmoLL) from Bauhinia monandra Kurz (Fabaceae) leaves on Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Dryophthoridae)","authors":"Thiago Lucas da Silva Lira ,&nbsp;Cintia Ferreira de Almeida ,&nbsp;Luis Afonso Silveira Farias ,&nbsp;Ewelyn Cintya Felipe dos Santos ,&nbsp;Magda Rhayanny Assunção Ferreira ,&nbsp;Luiz Alberto Lira Soares ,&nbsp;Thiago Henrique Napoleão ,&nbsp;Patrícia Maria Guedes Paiva ,&nbsp;Luana Cassandra Breitenbach Barroso Coelho","doi":"10.1016/j.bcab.2025.103893","DOIUrl":"10.1016/j.bcab.2025.103893","url":null,"abstract":"<div><div><em>Sitophilus zeamais</em> Motschulsky, 1855 (Coleoptera: Dryophthoridae) is a major insect pest of stored maize grains, whose control relies on synthetic insecticides that pose environmental risks and promote resistance development. Plant-derived compounds, including secondary metabolites and lectins, are promising alternatives for pest management. In this study, the insecticidal effects of saline extract (SE), protein fraction (PF), and lectin (BmoLL) from <em>Bauhinia monandra</em> Kurz leaves on mortality, feeding, and nutritional parameters of <em>S. zeamais</em> adults were evaluated via incorporation into artificial diets. <em>I</em><em>n vitro</em> effects on trypsin, protease, α-amylase, and acetylcholinesterase activities were also assessed. Phytochemical analysis revealed flavonoids, cinnamic acid derivatives, saponins, and reducing sugars in SE and PF, with rutin confirmed by high-performance liquid chromatography (HPLC). BmoLL exhibited two bands (33 and 27 kDa) in sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), confirming its presence in SE and PF and its successful purification. SE, PF, and BmoLL induced dose- and time-dependent mortality, with LC₅₀ values of 326.86, 119.50, and 7.00 mg/g at 7 days, and 227.39, 101.31, and 5.09 mg/g at 14 days, respectively. All samples caused feeding deterrence ranging from weak to strong and negatively affected nutritional parameters. SE and PF stimulated trypsin and protease activities, PF reduced α-amylase activity, whereas BmoLL increased it. SE and PF also enhanced acetylcholinesterase activity. In conclusion, SE, PF, and BmoLL exert lethal, antifeedant, antinutritional, and antimetabolic effects on <em>S. zeamais</em>, with BmoLL showing high efficacy at low concentrations, highlighting their potential as sustainable grain protection agents.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"71 ","pages":"Article 103893"},"PeriodicalIF":3.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Somatic hybridization: a Cinderella of plant biotechnology?","authors":"Elena Rakosy-Tican,&nbsp;Daniel Cruceriu,&nbsp;Imola Molnar","doi":"10.1016/j.bcab.2025.103875","DOIUrl":"10.1016/j.bcab.2025.103875","url":null,"abstract":"<div><div>Somatic hybridization is a plant biotechnological technique that belongs to genetic manipulation/improvement since it involves the genetic manipulation of naked plant cells or protoplasts. Protoplast from two different species or genotypes can be fused and somatic hybrid plants regenerated based on plant somatic cell totipotency. The first somatic hybrids were obtained and regenerated in the 1970s and many groups of crops and horticultural species have benefited from the isolation of protoplasts, their fusion and regeneration of improved somatic or parasexual hybrids. In some crops, such as potato, this genetic manipulation tool has produced good results with exploitation of wild <em>Solanum</em> species as donors of resistance genes. Still, there are many reviews on plant biotechnology that only discuss the most recent techniques, such as plant genetic transformation, RNAi and genome editing techniques, as successful plant biotechnologies. In this review we aim to discuss some of the reasons for the neglect shown to somatic hybridization, a technique able to bypass pre- and postzygotic sexual incompatibility barriers between related but incongruent species and to facilitate the introgression of multiple genes (i.e. QTLs) or multiple resistance traits into the gene pool of different crops. We also discuss the new role of this technique in the genomic era, and how this Cinderella of plant biotechnology can benefit plant breeding as a non-GMO technology in a complex, combinatorial biotechnological scheme under the urgent challenges of climate change and population growth.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"71 ","pages":"Article 103875"},"PeriodicalIF":3.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145838009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heavy metal tolerance, alkane biodegradation, and biosurfactant production by Chryseomicrobium amylolyticum RP2 isolated from industrial effluents","authors":"Ranjan Pandey ,&nbsp;Bhaskar Krishnamurthy ,&nbsp;Abdullah A. Alarfaj ,&nbsp;Mohammad Javed Ansari ,&nbsp;Daizy Rani Batish ,&nbsp;Harminder Pal Singh","doi":"10.1016/j.bcab.2025.103892","DOIUrl":"10.1016/j.bcab.2025.103892","url":null,"abstract":"<div><div>The current study examined the heavy metal tolerance, biodegradation potential, and biosurfactant production of a strain of bacteria isolated from industrial effluents. The 16S <em>r</em>RNA analysis identified the strain as <em>Chryseomicrobium amylolyticum</em> RP2. The strain RP2 exhibited a tolerance of at least 0.5 g/L towards various elements, including As⁵⁺, Fe²⁺, Mo⁶⁺, Pb²⁺, Co²⁺, Cu²⁺, Mn²⁺, Ni²⁺, and Zn²⁺. The isolate emulsified several organic substrates, such as aviation turbine fuel, diesel, and xylene, with an emulsification index above 60%. The fluorescence microscopy showed that the RP2 strain degrades ≥95% of the model hydrocarbons (<em>n</em>-hexadecane, diesel, and kerosene) within 14 days of incubation. The biosurfactant was rich in hexa-, octadecenoic, and octadecadienoic acids, and was stable at pH 6–10 and 20–60 °C temperature. NMR, FTIR, and MALDI-TOF analyzes showed that the biosurfactant was mostly lipid and peptide but also contained minor carbohydrate residues, making the compound more accurately described as lipopeptide-like, complex lipo-glyco-lipid-like, or mixed-type biosurfactants. At a critical micelle concentration of 0.380 mg/mL, the biosurfactant effectively reduced the surface tension of water to 29.56 mN/m. This study is the first to report the biosurfactant properties and remediation potential of a heavy metal-tolerant RP2 strain of <em>C. amylolyticum</em>. The results of the present study reflect as preliminary evidence that the <em>C. amylolyticum</em> RP2 strain, with its resistance to heavy metals and effective remediation capability, could remove organic pollutants; however, the presence of genes responsible for the degradation of alkanes and further investigation into the industrial applications of biosurfactant is necessary.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"71 ","pages":"Article 103892"},"PeriodicalIF":3.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145838011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenite seedling priming enhances cadmium tolerance in Solanum lycopersicum cv. Micro-Tom","authors":"Emilaine da Rocha Prado,&nbsp;Reginaldo de Oliveira,&nbsp;Mirela Vantini Checchio,&nbsp;Priscila Lupino Gratão","doi":"10.1016/j.bcab.2025.103887","DOIUrl":"10.1016/j.bcab.2025.103887","url":null,"abstract":"<div><div>Cadmium (Cd) severely impacts crops as one of the most deleterious metals, whereas selenium (Se) enhances plant defense mechanisms under stress conditions. Seedling priming represents a promising pre-conditioning strategy to improve plant tolerance, but the effectiveness of different Se sources against Cd stress remains poorly understood. This study investigated the effects of Se priming in <em>Solanum lycopersicum</em> cv. Micro-Tom seedlings with selenate or selenite on Cd tolerance. Seedlings were treated with 10 μM selenate or selenite from 10 to 20 days after planting (DAP), and Cd stress (0.5 mM) was applied at 40 DAP. Growth, pigment contents, malondialdehyde (MDA), and hydrogen peroxide (H₂O₂) levels were evaluated. Cd stress reduced chlorophyll <em>a</em>, chlorophyll <em>b</em>, and carotenoids by 64.7 %, 56.6 %, and 54.8 %, respectively, and decreased leaf, stem, and root dry mass by 32.2 %, 52.9 %, and 30.0 %. Selenite priming mitigated these effects, reducing MDA by 87 % in leaves and 58 % in roots, while increasing leaf dry mass by 12 % and chlorophylls and carotenoids by up to 74 % under Cd stress. In contrast, selenate increased root dry mass but showed limited effectiveness in reducing oxidative stress markers. Principal component analysis (PCA) showed clear separation of treatments, with selenite + Cd clustering with tolerant responses and Cd-only positioned with stress indicators. This is the first study to demonstrate the efficacy of seedling priming with selenite (not selenate) in Micro-Tom tomato, highlighting its potential for improving crop tolerance to Cd stress.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"71 ","pages":"Article 103887"},"PeriodicalIF":3.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-enclosure black soldier fly system for decentralized conversion of mixed organic residues into feed-grade larvae and stabilized frass","authors":"Rachana D. Sharma ,&nbsp;Anupa Velusamy ,&nbsp;Prabhu Thangadurai","doi":"10.1016/j.bcab.2025.103905","DOIUrl":"10.1016/j.bcab.2025.103905","url":null,"abstract":"<div><div>This study demonstrates a low-cost, single-enclosure black soldier fly (BSF, <em>Hermetia illucens</em>) biopod for decentralized conversion of mixed organic residues into feed-grade larvae and stabilized frass. We evaluated 15-day cycles on kitchen waste (KW) and farm waste (FW), measuring mass reduction, larval yield, and agronomic performance. In controlled runs, dry-matter reduction averaged 79 ± 7 % (KW) and 65 ± 6 % (FW). Larval yield was 12 ± 2 % and 10 ± 2 % of input dry matter (DM), respectively, corresponding to recovery of roughly one-tenth of the incoming dry solids as harvestable larvae. Average daily wet-mass reduction was ≈5 % d⁻¹ (KW) and ≈4 % d⁻¹ (FW). In 20 household and small-farm deployments, users received brief initial training and then operated the system independently for consecutive 15-day cycles. Performance stabilized at 73 ± 6 % dry-matter reduction and 11 ± 2 % larval yield, and 90 % of users reported willingness to continue operating the unit, indicating reproducibility by non-specialists. Sun-dried larval meal contained ≈21–29 % protein and ≈21–31 % lipid, lauric-rich (C12:0 dominant). Stabilized frass met maturity criteria (germination index ≈ 90 %), had near-neutral pH, and contained total N ≈ 3.2–3.5 % DM. When applied on a plant-available nitrogen basis in greenhouse pots, frass supported tomato, brinjal, and chilli yields that were statistically comparable to mineral fertilizer at 150 kg N ha⁻¹ and consistently higher than compost adjusted to the same plant-available nitrogen. Taken together, these results provide a practical, scalable pathway for rapid on-site waste attenuation with concurrent production of locally useful feed and fertilizer, advancing nutrient circularity in resource-constrained settings.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"71 ","pages":"Article 103905"},"PeriodicalIF":3.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145838014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity of aqueous extract of Crotalaria stipularia (Desv., 1814) against the Annona fruit borer Cerconota anonella (Sepp, 1830) (Lepidoptera: Oecophoridae)","authors":"Camila Pereira de Lima Chicuta ,&nbsp;Andréa Carla de Almeida Barros ,&nbsp;Marta Angelo dos Santos ,&nbsp;Ruth Rufino do Nascimento ,&nbsp;Josiel Santos do Nascimento ,&nbsp;Luciano Aparecido Meireles Grillo ,&nbsp;Jeinny Christine Gomes Barros ,&nbsp;Orlando Francisco da Silva Moura ,&nbsp;Johnnatan Duarte de Freitas ,&nbsp;Francis Soares Gomes","doi":"10.1016/j.bcab.2025.103909","DOIUrl":"10.1016/j.bcab.2025.103909","url":null,"abstract":"<div><div>The <em>Annona</em> fruit borer, <em>Cerconota anonella</em> (Sepp., 1830) (Lepidoptera: Oecophoridae), is a major pest of pine and soursop fruits, and its control commonly relies on synthetic pesticides that pose risks to non-target organisms. This study aimed to characterize the chemical composition of the aqueous seed extract of <em>Crotalaria stipularia</em> and evaluate its insecticidal activity against adult <em>C. anonella</em>. The crude extract was prepared in 50 mM Tris-HCl buffer (pH 8.0), quantified for protein content by the Bradford method, and tested for hemagglutinating activity to confirm the presence of lectins. The phytochemical profile was determined by high-performance liquid chromatography (HPLC). Bioassays were conducted using artificial diets containing 30, 45, or 90 μg of extract protein per mL to assess mortality, feeding deterrence, nutritional parameters, and biochemical changes in the insects. HPLC analysis identified chlorogenic acid (2.8642 mg/kg) and syringaldehyde (0.2763 mg/kg) in the extract. The highest concentration (90 μg protein/mL) caused 50 % mortality after three days and significantly reduced relative growth rate, food conversion efficiency, and the levels of proteins, glucose, cholesterol, and triglycerides in the insects. These findings indicate that <em>C. stipularia</em> seed extract disrupts insect metabolism and exhibits promising potential as an eco-friendly alternative for the management of <em>C. anonella</em>.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"71 ","pages":"Article 103909"},"PeriodicalIF":3.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145838015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the growth and productivity performances of Eggplant (Solanum melongena L.), Varieties Grown under water stress conditions","authors":"Dina Maachi,&nbsp;Mariam Oussellam,&nbsp;Kamal Aberkani","doi":"10.1016/j.bcab.2025.103902","DOIUrl":"10.1016/j.bcab.2025.103902","url":null,"abstract":"<div><div>Water scarcity is a limiting factor impacting plant growth, yield, and photosynthesis, leading to reduced agricultural production in the arid and semi-arid regions of the world. This study aims to evaluate the growing performance of nine commercial varieties of eggplant (<em>Solanum melongena L</em>.), under water stress conditions. Two types of irrigation treatments were used: 100 % (irrigation made by the grower) and 50 %. The field experiment was conducted in 2022 in the Mediterranean area of northeastern Morocco. The design of this study was completely randomized and contained blocks and with five replications for each variety for each irrigation treatment. Various physio-morphological parameters were recorded, such as growth parameters, relative water content (RWC), root biomass, fruit quality, chlorophyll <em>a</em> fluorescence (Fv/Fm, PIabs, PItotal, DI0/CS0 and ABS/CS0) and yield parameters. The main results showed highly significant differences among the varieties under water stress treatment. Plant height reductions ranged from 1.83 % to 13.36 % for the all the varieties, with ‘Tamara’ exhibited a 28.85 % reduction in flower production. The RWC decreased by 26.8 % in ‘Tamara’, Root biomass was reduced by 10.3 %–40.7 % in most eggplant varieties under water deficit stress. ‘Chinoise’ variety showed high tolerance to water shortage with a higher number of fruits and yield under the two treatments. Conversely, ‘Aswab’ variety presented a significant sensibility to water deficit, as evidenced by its production of the lowest number of fruits. The yield of ‘Samara’ decreased by 37.32 %, marking the highest reduction among the varieties studied. These studies highlight the importance of selecting water scarcity tolerant varieties of eggplants under the semi-arid climate of the Mediterranean area.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"71 ","pages":"Article 103902"},"PeriodicalIF":3.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145838066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}