Biocatalysis and agricultural biotechnology最新文献

{"title":"Solid-state fermentation with Bacillus subtillis co-cultured with probiotic Lactobacillus spp. enhances the bioactive peptides, nutritional and antioxidative potentials of tamarind seed","authors":"","doi":"10.1016/j.bcab.2024.103368","DOIUrl":"10.1016/j.bcab.2024.103368","url":null,"abstract":"<div><p>The effect of natural or controlled solid-state fermentation using single or mixed probiotic strains on the nutritional, bioactive and phytochemical constituents, and antioxidant activities of tamarind seeds was evaluated. Tamarind seeds were subjected to either controlled solid-state fermentation (37 °C, 72 h) using either <em>Bacillus subtilis</em> only or <em>Bacillus subtilis</em> co-cultured with probiotics <em>Lactobacillus</em> spp. (<em>Lactobacillus fermentum, Lactobacillus rhamnosus GG, Lactobacillus brevis,</em> and <em>Lactobacillus delbrueckii</em>) or natural fermentation while unfermented tamarind seeds served as control. The changes in the nutritional composition, peptide content, antioxidative properties (Ferric reducing antioxidant power (FRAP) and DPPH radical scavenging activities) and total viable count of probiotics in fermented tamarind seed were evaluated using standard methods. The peptide content, crude protein, fat, and fibre (100.19 mg/g, 51.35, 9.50 and 5.10%), and FRAP and DPPH (0.55 and 21.60%) in unfermented tamarind seed were significantly (p &lt; 0.05) lower than fermented tamarind seed (271.00–308 mg/g, 12, 62.84–64.02, 12.21–13.11 and 6.01–6.90, and 1.42–2.92 and 26.66–35.70%, respectively), after 72 h of fermentation. The total viable count in the tamarind seed fermented with <em>Bacillus subtilis</em> only was 1.2 × 10⁹ CFU/g while that co-cultured with probiotics ranged from 6 × 10⁸ to 9.9 × 10⁸ CFU/g after 72 h of fermentation. This study showed that a controlled solid-state fermentation using probiotics <em>Lactobacillus</em> co-cultured with <em>Bacillus subtilis</em> can significantly increase the peptide contents, and enhance the nutritional and antioxidant activities of tamarind seed while producing a condiment with significant probiotic potential.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potato and dairy industry side streams as feedstock for fungal and plant cell cultures","authors":"","doi":"10.1016/j.bcab.2024.103367","DOIUrl":"10.1016/j.bcab.2024.103367","url":null,"abstract":"<div><p>Utilization of industrial side streams as nutrient source for cellular agriculture is a promising option to improve the sustainability of the production processes. The aim of the study was to evaluate usability of two liquid food industry side streams, potato cell fluid and acid whey, as nutrient source for production of food ingredients with plant cell cultures (arctic bramble (<em>Rubus arcticus</em> L.) and tobacco BY-2 (<em>Nicotiana tabacum</em> L.)) and filamentous fungi (<em>Paecilomyces variotii</em>, <em>Rhizopus oligosporus</em> and <em>Trichoderma reesei</em>). The side streams were found to contain various sugars and other potential nutrients suitable for the studied organisms. The side streams were used as a sole nutrient source (fungi) or as a replacement of selected nutrients in the growth media (fungi and plant cells) in flask scale cultivations. Acid whey was successfully used as a growth medium for filamentous fungi, but it inhibited plant cell growth presumably due to high organic acid content (14 g/l). Potato side stream was found suitable as a fungal and plant cell growth media supplement, and it was used together with glucose for filamentous fungi, or as a partial replacer of macronutrients for plant cells, in bioreactor cultivations yielding high fungal (up to 36 g/l dry weight) and good plant cell (9.5 g/l dry weight) biomass production. Analyses of the produced biomasses revealed good nutritional value in terms of amino acid (17–27 % of dry matter) and dietary fiber (23–30 % of dry matter) contents, giving good premises for utilization in human nutrition.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1878818124003517/pdfft?md5=5318a5d63e6b5486563d9efcfad6b71b&pid=1-s2.0-S1878818124003517-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization, isolation, identification and molecular mechanisms in B16F10 melanoma cells of a novel tyrosinase inhibitory peptide derived from split gill mushrooms","authors":"","doi":"10.1016/j.bcab.2024.103363","DOIUrl":"10.1016/j.bcab.2024.103363","url":null,"abstract":"<div><p>Hyperpigmentation often arises from an imbalance in melanogenesis, primarily due to the overexpression of tyrosinase (TYR). While the inhibition of TYR presents a common approach to skin whitening, it can lead to undesirable side effects. Thus, there is growing interest in safe and natural alternatives for TYR inhibition. Bioactive compounds and peptides sourced from split gill mushrooms hold promise in this regard. This study aims to optimize the conditions for papain-mediated hydrolysis of split gill mushroom protein to inhibit TYR activity, utilizing response surface methodology (RSM) and central composite design (CCD). Optimal conditions were determined at a temperature of 46.70 °C, a hydrolysis time of 217.09 min, and an enzyme-to-substrate ratio (E/S) of 1.1%. Under these conditions, the resulting hydrolysates exhibited significant TYR inhibition, with an IC₅₀ value of 117.86 μg/mL and a degree of hydrolysis (DH) of 87.97%. Further purification <em>via</em> ultrafiltration and RP-HPLC yielded a peptide, Tyr-Ala-Ser-Ile-Leu-Leu (YASILL or YL-6), identified through LC-Q-TOF-MS/MS, which competitively inhibited TYR. YL-6 demonstrated an IC₅₀ value of 3.97 mM for mono-phenolase activity and 6.75 mM for di-phenolase activity. Molecular docking analysis revealed hydrogen bonds and hydrophobic interactions between TYR and YL-6. Treatment of B16F10 cells with YL-6 across concentrations ranging from 10-3000 μM showed no cytotoxic effects.The inhibition of melanin synthesis was investigated <em>via</em> qRT-PCR along with Western blot in MITF, TYR, TRP-1, and TRP-2. The results obtained in this research may prove significant in guiding the development of commercially viable cosmetic products to whiten the skin.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effect of biological pre-treatment on co digestion of rice straw and sewage sludge: Process optimization and microbial interactions","authors":"","doi":"10.1016/j.bcab.2024.103364","DOIUrl":"10.1016/j.bcab.2024.103364","url":null,"abstract":"<div><p>The increasing demand for renewable energy sources has prompted a search for innovative and efficient approaches for biogas production. This study investigates the potential of achieving sustainable biogas production through a synergistic combination of biological pre-treatment and co-digestion of rice straw (RS) and sewage sludge (SS). The goal is to enhance the overall methane yield, mitigate substrate limitations, and optimize the biogas production process. In this study, rice straw and sewage sludge were anaerobically co-digested in ratios of 100:0, 70:30, 50:50, 30:70, and 30:70. It was observed that the co-digestion ratio of 70:30 is optimal to achieve maximum methane yield of 0.3 L CH₄/(g VS added). Biological pre-treatment with five different organisms <em>Sphingobium</em> sp.<em>, Paenibacillus</em> sp.<em>, Microbacterium</em> sp.<em>, Pseudomonas</em> sp.<em>, and Stenotrophomonas</em> sp. was also examined for RS and co-digested with SS at the optimized ratio to improve the degradability of RS. This pre-treatment strategy is anticipated to enhance the accessibility of microorganisms to substrates and accelerate the rates of hydrolysis. The biological pre-treatment resulted in a 20–23% improvement in methane yield compared to untreated substrates. Metagenomic studies revealed the dominance of <em>Bacteroidetes, Firmicutes, and Proteobacteria</em>, which are capable of utilizing lignocellulosic biomass and ultimately converting it to methane. The acetoclastic methanogensis is the major methane generating pathway which is supported by the complete dominance of genus <em>Methanosaeta.</em></p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142270915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and antioxidant activity of eumelanin produced by Streptomyces lasalocidi NTB 42","authors":"","doi":"10.1016/j.bcab.2024.103361","DOIUrl":"10.1016/j.bcab.2024.103361","url":null,"abstract":"<div><p>Melanin, a macromolecule derived from the oxidative polymerization of phenolic compounds, possesses notable bioactive attributes and serves as a crucial biological safeguard against deleterious environmental factors. Melanin originates in <em>Streptomyces</em> and is known for its antioxidative properties. This study aimed to characterize and evaluate the antioxidant activity of melanin obtained from <em>Streptomyces lasalocidi</em> NTB 42. Based on 16 S rRNA identification, strain NTB 42 was identified as <em>Streptomyces lasalocidi</em> NTB 42 (OR710932). During melanin biosynthesis, <em>S. lasalocidi</em> NTB 42 had the highest tyrosinase activity on day 5 and the highest L-3,4-dihydroxyphenylalanine concentration on day 6. Melanin NTB 42 exhibited polymorphic and irregular morphology. Melanin NTB 42 characteristics were analyzed using the A₆₅₀/A₅₀₀ ratio, UV–visible spectroscopy, Fourier transform infrared spectroscopy, high-performance liquid chromatography, dispersive X-ray spectroscopy, and thermal degradation products based on pyrolysis-gas chromatography-mass spectrometry were identified as eumelanin. The scavenging ability of NTB 42 melanin, as determined by the half maximal inhibitory concentration (IC₅₀), was 70.60 ± 0.40 μg/mL for 2,2-diphenyl-1-picrylhydrazyl and 176.76 ± 0.93 μg/mL for 2,2′-azino-bis(3-ethylbenzothiazolin-6-acid sulfonate radicals. The Fe-reducing power and total antioxidant capacity values were 114.05 ± 0.41 mg AAE/g melanin and 145.10 ± 0.56 mg AAE/g melanin, respectively. This study is the first attempt to characterize the antioxidant properties of eumelanin from <em>S. lasalocidi</em> NTB 42. The antioxidant ability of eumelanin NTB 42 against various free radicals shows promise for further development as an antioxidant.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142270920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of chitosan nanoparticles on seedling and germination attributes and enzymatic activity of guar (Cyamopsis tetragonoloba L.) under osmotic stress","authors":"","doi":"10.1016/j.bcab.2024.103356","DOIUrl":"10.1016/j.bcab.2024.103356","url":null,"abstract":"<div><p>This research was conducted to examine the effects of nano chitosan particles on seed germination and seedling growth of guar (<em>Cyamopsis tetragonoloba</em> L.) under osmotic stress conditions. This experiment was conducted in a factorial layout based on a completely randomized design with two factors and four replications. The experimental factors included: osmotic stress at five levels (0, −3, −6, −9 and −12 bar) and seed priming with nano chitosan particles at five concentrations (0, 50, 100, 200, and 300 ppm). The evaluated parameters in this study were alometric traits and enzymatic activities including proline, catalase, ascorbate peroxidase, superoxide dismutase, and total soluble sugar, as well as the content of chlorophyll <em>a, b</em>, and carotenoids. The results indicated the significant effect of most of the studied quantitative and qualitative traits. The highest values of seedling, plumule and radicle length (48.20, 17.79 and 30.15 mm, respectively), and the fresh weight of seedlings and plumule (0.9743 and 0.9192 g, respectively) were observed at a concentration of 200 ppm of chitosan nanoparticles. The highest amount of all studied parameters observed under non-stress conditions (control), and the lowest values of them obtained in −12 bar of osmotic stress. Furthermore, by increasing of osmotic stress levels, germination percentage and rate and seed vigor showed a decreasing trend about 56, 83 and 95% compared to the control. Besed on the interaction effects, the maximum values of seedling and plumule length were achieved with the application of 200 ppm chitosan nanoparticles in combination with no osmotic stress. Under non-osmotic conditions, concentrations of 300, 100, and 200 ppm chitosan led to the highest values of germination percentage and rate and seed vigor, respectively. Additionally, the highest percentage of normal seedlings (94%) and the lowest percentage of abnormal seedlings (zero) were obtained under the application of 200 ppm of nano chitosan and no osmotic stress. In relation to qualitative traits, the lowest values of soluble sugar and proline were obtained at the osmotic level of −12 bar. Moreover, the application of 200 ppm of nano chitosan and −6 bar of osmotic stress resulted in the highest levels of catalase, superoxide dismutase, ascorbate peroxidase, chlorophyll <em>a, b</em>, and carotenoids. As a whole, the results of the study demonstrated that seed priming with chitosan nanoparticles can reduce the negative effects of drought stress on guar seedlings. In addition, application of 200 ppm of this substance was the most suitable treatments that improve most quantitative and qualitative indices of guar. <strong>Keywords</strong>: Enzymatic activity, Nano particles, Priming, Seed structure.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142270919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-process optimization for developing a turmeric (Curcuma longa Linn.) beverage fermented with functional lactic acid starter cultures","authors":"","doi":"10.1016/j.bcab.2024.103360","DOIUrl":"10.1016/j.bcab.2024.103360","url":null,"abstract":"<div><p>Turmeric, scientifically known as <em>Curcuma longa</em>, is a herbaceous plant characterized by its rhizomatous nature with remarkable chemical composition and biologically active compounds. Lactic acid bacteria (LAB) have been observed to break down complex polyphenols into simpler, more bioactive compounds through fermentation. The success of the fermentation process relies on various factors to achieve the desired end product. This study's main goal was to assess the feasibility of using turmeric as a fermentable substrate for LAB, with the intention of creating a non-dairy fermented beverage. The investigation delved into the impact of several fermentation parameters, namely Fermentation Temperature, pH (Lemon juice concentration), Inoculum concentration, and Substrate concentration (Turmeric concentration), on the phytochemical and antioxidant properties of the lactic acid-fermented turmeric beverage (FTB). Statistical analysis revealed that a quadratic polynomial model could effectively illustrate how these fermentation parameters influenced the beverage's phytochemical and antioxidant qualities. Visualization through response surface plots revealed that these independent variables significantly influenced the beverage's total phenolic content, total flavonoid content, and antioxidant properties. Optimal fermentation conditions were identified to yield the highest levels of phenolic, flavonoid, and antioxidant content. These conditions included a Fermentation Temperature of 37.45 °C, a pH of 5.62, an Inoculum concentration of 5.12%v/v, and a Substrate (turmeric) concentration of 2.28%w/v. Under these precise conditions, experimental results closely aligned with predicted values for total phenolic content (57.46mg/100 ml), total flavonoid content (51.48mg/100 ml), and total antioxidant activity (72.24%). The overall acceptability of the fermented turmeric beverage reached a high 98.10%. Notably, the FTB exhibited significantly improved attributes such as astringency, flavor, taste, and overall acceptability when compared to the non-fermented turmeric beverage (NFTB), with statistical significance (P &lt; 0.05).</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vivo toxicological study of cysteamine modified carbon dots derived from Ruellia simplex on fruit fly for potential bioimaging","authors":"","doi":"10.1016/j.bcab.2024.103359","DOIUrl":"10.1016/j.bcab.2024.103359","url":null,"abstract":"<div><p>Modern medicine must shift its paradigm from conventional treatment strategies to theranostics to meet individuals' needs. Carbon dots, as a class of fluorescent materials, provide biocompatible and multifunctional solutions for a wide range of applications including clinical sensing, imaging, and drug delivery. Various studies have focused on their synthesis, photophysical properties, and innoxious nature but their potential biomedical applications for the <em>in-vivo</em> toxicity are yet limited. In this work, previously synthesized C-Dots (CDs) were utilized to determine their effect on the development of <em>Drosophila melanogaster</em>. Simultaneously, the genotoxic potential of CDs was evaluated on specific larval cell types that play important roles in immunological defence as well as growth and development. The gut organ toxicity of both CDs was studied using DAPI and DCFH-DA dyes wherein RS-CDs didn't show significant toxicity to the concentration 500 μg/mL whereas RS-Cys-CDs showed nuclear fragmentation and modest ROS (reactive oxygen species) production. Subsequently, trypan-blue assay, larvae crawling assay, touch sensitivity, adult phenotype, and survivability assay were performed. The trypan-blue assay shows the non-toxic nature of both CDs even at the concentration of 500 μg/mL. The high concentrations of RS-Cys-CD (500 μg/mL) were further associated with the alteration in touch behaviour and decrease in pupa hatching. <em>In-vivo and ex-vivo</em> fluorescence assessment of both the CDs exhibit bright fluorescence in green and red channels upon excitation at 485 and 577 nm respectively. The prominent imaging results from RS-Cys-CDs highlight the positive impact of surface modification.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring soil microbiota and their role in plant growth, stress tolerance, disease control and nutrient immobilizer","authors":"","doi":"10.1016/j.bcab.2024.103358","DOIUrl":"10.1016/j.bcab.2024.103358","url":null,"abstract":"<div><p>Agriculture and the human population have historically thrived together; however, the exponential growth of the human population has now surpassed the capacity of natural resources to meet global food demands. Current agricultural practices are increasingly compromising environmental and ecosystem health. Traditionally, agricultural practices aimed to maximize output with minimal input. However, the relentless drive for higher production, coupled with resource overuse and an insatiable demand for increased yields, has initiated a detrimental cycle, ultimately resulting in the need for increased inputs. This cycle includes diminishing returns, increased input demands, environmental degradation, and a perpetual escalation of input requirements. Specifically, higher input use leads to the repeated application of chemical fertilizers to enhance yields and the expansion of farmland at the cost of natural habitats. This expansion also involves extensive pesticide use to combat diseases, resulting in significant biodiversity loss. To address the rising demands while mitigating health concerns and striving for sustainable agriculture, researchers propose an integrated solution: the use of plant growth-promoting rhizobacteria (PGPR) alongside traditional agrochemicals. Utilizing PGPR in a balanced manner as both bio-fertilizers and bio-pesticides offers a holistic approach to sustainable agriculture. This strategy enhances stress tolerance, promotes growth, increases yields, serves as a biocontrol agent, suppresses diseases, and immobilizes nutrients, all while being eco-friendly. However, bridging the gap between research, formulation, and commercialization of PGPR remains essential for its successful application in agriculture. A comprehensive understanding of microbial ecology, metatranscriptomics, biotechnology, and rhizo-engineering is proposed to optimize the efficacy of these bio-products to their fullest potential.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142270918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of trophic mode and medium composition on the biochemical profile and antioxidant capacity of Tetraselmis chuii (CCAP 66/21B)","authors":"","doi":"10.1016/j.bcab.2024.103362","DOIUrl":"10.1016/j.bcab.2024.103362","url":null,"abstract":"<div><p>Microalgal cultivation influences the bioaccumulation of high-value compounds such as omega-3 (ω-3) fatty acids and carotenoid pigments. Therefore, cultivation optimisation is essential to upregulate high-value compound yields. The present study investigated the effects of trophic mode (autotrophy, heterotrophy and mixotrophy) and media composition on the biochemical make-up, pigment signature, fatty acid profile, and antioxidant capacity of the marine chlorophyte, <em>Tetraselmis chuii</em> (CCAP 66/21 B). The 13 conditions significantly affected the biochemical profile of <em>T. chuii</em> (CCAP 66/21 B) with high variation in carbohydrates (78.5–151.7 mg glucose eq g⁻¹dw), lipids (208.3–475.1 mg g⁻¹dw) and soluble proteins (47.3–373.9 mg BSA eq g⁻¹dw). Trophic mode influenced the yields of high-value nutraceutical carotenoids (lutein and β-carotene) with the highest returns observed in photoautotrophic conditions (e.g., 2.51 ± 0.11 mg β-car g⁻¹dw and 1.96 ± 0.14 mg lut g⁻¹dw for K medium). Organic supplementation also induced significant shifts in the proportions of polyunsaturated fatty acids (ω-3 and ω-6 PUFAs), monounsaturated fatty acids (MUFAs) and saturated fatty acids (SFAs). Here, heterotrophy and mixotrophy significantly upregulated MUFA content (33.2 ± 4.0%) and SFA content (31.29 ± 0.9%), respectively. Moreover, mixotrophy significantly enhanced biomass yield (6.3-fold), soluble protein content (3.9-fold) and the antioxidant capacity (5.2-fold) of <em>T. chuii</em> (CCAP 66/21 B) compared to obligate photoautotrophy and heterotrophy. As such, trophic mode is a principal growth parameter that can modulate the content of <em>T. chuii</em> (CCAP 66/21 B) for potential downstream applications such as biofuels, pharmaceuticals, nutracurticals or aquaculture probiotics.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}