Impact of honey-enriched soursop leaves (Annona muricata) kombucha on lipid profiles and hypoglycemic properties: An in-vivo study

This study investigated the hypoglycemic and lipid-modulating effects of honey-enriched soursop (Annona muricata) leaves kombucha in alloxan-induced diabetic Wistar rats. Diabetes was induced using alloxan monohydrate (45 mg/kg BW), followed by 28 days of oral administration of kombucha at 0.5 %, 1.0 %, or 1.5 % (w/v; 5 mL/kg BW/day), metformin (45 mg/kg BW/day), or no treatment. The 1.5 % kombucha dose markedly reduced blood glucose from 224.4 ± 4.77 to 99.4 ± 7.82 mg/dL, comparable to metformin (107.4 ± 7.43 mg/dL). It also improved lipid profiles, lowering total cholesterol (59.0 ± 5.33 mg/dL), triglycerides (34.6 ± 8.32 mg/dL), and LDL (10.0 ± 7.10 mg/dL), while elevating HDL (47.6 ± 7.76 mg/dL). Histological examination revealed pancreatic islet regeneration in treated groups. In vivo toxicity evaluation using Zophobas morio larvae showed no adverse effects. These results demonstrate that honey-enriched soursop leaves kombucha is a safe and effective natural candidate for managing diabetes and dyslipidemia.