Biocatalysis and agricultural biotechnology最新文献

{"title":"Repurposing metal oxide nanoparticles for enhanced xylanase production by Aspergillus niger in corncob-based media: Taguchi optimization and paper recycling application","authors":"Agbaje Lateef ,&nbsp;Joseph Adetunji Elegbede ,&nbsp;Evariste Bosco Gueguim-Kana ,&nbsp;Lorika Selomi Beukes ,&nbsp;Ntombozuko Matyumza","doi":"10.1016/j.bcab.2025.103585","DOIUrl":"10.1016/j.bcab.2025.103585","url":null,"abstract":"<div><div>The economic xylanase production for industrial applications cannot be underestimated due to its large global market size. This study therefore explores the potentials for enhanced xylanase production by a local strain of <em>A. niger</em> using Taguchi optimization involving metal oxide nanoparticles. The xylanases were thereafter assessed for pulp wastewater biodeinking and pulp biobleaching. Zinc oxide (XA-ZnONPs) and calcium oxide nanoparticles (XA-CaONPs) were biosynthesized by xylanase and characterized through UV–Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR) and Transmission electron microscopy (TEM). Both biosynthesized XA-ZnONPs and XA-CaONPs displayed characteristic surface plasmon resonance peak at 314 and 370 nm, respectively. The FTIR revealed amine-rich functional groups as prevalent in the nanoparticles and TEM revealed their sizes as within 10.13–38.79 nm. Taguchi optimization resulted in enhanced fungal xylanase yield of 3.61-fold with XA-ZnONPs (103.55 U/ml) and 14.57-fold with XA-CaONPs (418.09 U/ml), whereby the metal oxide nanoparticles contributed 74.17–85.47 % to enhanced enzyme titers. The xylanases achieved 78.21–86.25 % ink removal efficiency in paper pulp effluent, while for the pulp, ink detachment efficiency of 37.04–82.70 % was recorded. The significant enhanced xylanase production and the remarkable deinking potential, are positive research efforts towards achieving economic xylanase production which can be applied in eco-friendly paper recycling. To the best of our knowledge, this is the first report for nanosupplementation for xylanase production by <em>A. niger</em>, establishing the relevance of nanotechnology for bioprocess optimization.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103585"},"PeriodicalIF":3.4,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the potential of seaweed as a source of lutein: A study of seasonal and spatial variation insights along the south-eastern coast of India","authors":"Venkatesan Ajithkumar ,&nbsp;V. Isaimozhi ,&nbsp;Sundarraj Dinesh Kumar ,&nbsp;S. Gopala Krishnan ,&nbsp;U. Gurumoorthy ,&nbsp;M. Avinesh ,&nbsp;V. Veeragurunathan ,&nbsp;Ramalingam Dineshkumar","doi":"10.1016/j.bcab.2025.103576","DOIUrl":"10.1016/j.bcab.2025.103576","url":null,"abstract":"<div><div>Seaweed play a major role in the food, feed, fertilizer, and cosmetic industries due to their distribution worldwide with varieties, along with their distinctive nutritional and pharmaceutical properties. Lutein is a second most high-value commercial carotenoid and seaweed could serve as a potential alternative to marigold flowers, entirely avoiding freshwater footprint and fertile land-usage. The present study is a first attempt on prospecting lutein production by various seaweed collected from crucial seaweed diversity sites along the south-eastern Indian coast during all the seasons. A total of 347 seaweed including 66 different species were collected during the study period comprising the four seasons. About 102 algae were observed in the pre-monsoon, followed by post-monsoon with 90 seaweed, 78 and 77 algae in the monsoon and summer, respectively. Out of 347 seaweed, lutein yields were prominent in 126 algae belonging to Chlorophyta, 122 to Rhodophyta, and 79 to Heterokontophyta. Among all the seaweed, higher lutein yields were observed for green seaweed such as <em>Valoniopsis pachynema</em> (3.8 mg g⁻¹<em>)</em>, <em>Ulva intestinalis</em> (2.8 mg g⁻¹)<em>, Caulerpa taxifolia</em> (2.2 mg g⁻¹), and <em>Ulva lactuca</em> (1.3 mg g⁻¹); followed by red algae like <em>Gelidium pusillum</em> (2.1 mg g⁻¹)<em>,</em> and <em>Gracilaria corticata</em> (0.74 mg g⁻¹)<em>.</em> To avoid over-exploitation of natural resources, seaweed that are cultivable and rich in lutein such as species of <em>Ulva</em>, <em>Caulerpa</em>, <em>G. pusillum</em> and <em>G. corticata</em> shall be considered for large-scale cultivation either in tank-systems or rafts deployed in the open sea for production of seaweed biomass-enriched in lutein, and other valuable compounds.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103576"},"PeriodicalIF":3.4,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxic evaluation of Cissus rotundifolia against four cancer cell lines and its phytochemical analysis by LC-MS/MS and GC-MS","authors":"Nahed O. Bawakid ,&nbsp;Bushra Y. Madkhali ,&nbsp;Lateefa A. Al-Khateeb ,&nbsp;Hajer S. Alorfi ,&nbsp;Kamel H. Shaker","doi":"10.1016/j.bcab.2025.103578","DOIUrl":"10.1016/j.bcab.2025.103578","url":null,"abstract":"<div><div><em>Cissus rotundifolia</em> has demonstrated a broad spectrum of biological activities and has been traditionally used in the treatment of several diseases. The cytotoxic effect of the <em>Cissus rotundifolia</em> grown in Saudia Arabia was evaluated against four human cancer cell lines; PACA2 (pancreatic), A549 (lung) line, SKOV3 (human ovarian) and HL-60 (leukemia). Both the ethanol extract and ethyl acetate fraction showed moderate activity against PACA2 with IC₅₀ values of 51.3 μg/ml and 57.9 μg/ml respectively. While the cytotoxic activity against SKOV3 was lower, with IC₅₀ values of 82.1 μg/ml and 77.9 μg/ml respectively. The ethyl acetate fraction exhibited significant activity against HL-60 cell line, with IC₅₀ value of 54.3 μg/ml. On the other hand, the <em>n</em>-hexane fraction showed moderate cytotoxicity against leukemia HL-60 with IC₅₀ value of 75.8 μg/ml, and lower activity against PACA2, while no activity was detected against SKOV3. Additionally, there was no detected activity of any of the tested fractions against A549 cell line. A phytochemical analysis was performed on both ethyl acetate and <em>n</em>-hexane fractions. The ethyl acetate fraction analysis was performed using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) revealing the presence of 16 metabolites with flavonoids as major metabolites. While the analysis of <em>n</em>-hexane fraction was performed by gas liquid chromatography coupled with mass spectrometry (GC-MS) revealing the presence of 26 compounds, with phytol and β-sitosterol as major compounds. The present study shows that <em>C. rotundifolia</em> and its fractions could contribute to pharmaceutical industries for the treatment of leukemia and pancreatic cancers.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103578"},"PeriodicalIF":3.4,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Burkholderia sp. EIKU24-derived selenium nanoparticles: Characterization, multifunctional bioactivities and their role in sustainable rice cultivation against arsenic stress","authors":"Nilendu Basak ,&nbsp;Atif Aziz Chowdhury ,&nbsp;Sayantani Paul ,&nbsp;Taniya Roy ,&nbsp;Sk Imran Ali ,&nbsp;Ekramul Islam","doi":"10.1016/j.bcab.2025.103575","DOIUrl":"10.1016/j.bcab.2025.103575","url":null,"abstract":"<div><div>The current study employed a rice root plaque-associated bacterium, <em>Burkholderia</em> sp. EIKU24, with the competency to synthesize spherical and crystalline biogenic selenium nanoparticles (BioSeNPs) that have size variability between 230 and 330 nm, as confirmed by SEM and TEM analysis. FT-IR and electron microscopy further revealed a biomolecular coating around the NPs that might have contributed significantly to their antibacterial activity against potential pathogenic Gram-positive and Gram-negative bacteria. The BioSeNPs suspension (10 mg mL⁻¹) inhibited 78 % and 67 % of <em>Staphylococcus aureus</em> and <em>Pseudomonas aeruginosa</em> biofilms, respectively. Furthermore, BioSeNPs showed very high antioxidant capability, reflected by 90 % relative DPPH scavenging activity and photocatalytic capability by the degradation of 86 % methylene blue (10 mg L⁻¹) solution within a contact time of 30 min. Compared to hydro or Na₂SeO₃ priming, rice seeds from BioSeNPs-priming outperformed in all tested seed germination parameters. Hydroponic cultivation showed better health and growth of the rice plants by an increase in root and shoot lengths, wet and dry biomass, and chlorophyll content, both in arsenic (As)-exposed and unexposed seedlings emerging from BioSeNPs-primed seeds. Notably, in such seedlings, exposure to As did not alter the growth much, indicating increased resilience to As through BioSeNPs priming. Besides, the priming of BioSeNPs decreased the translocation of As to shoot and root by about 50 % compared with hydro priming. The bioactivities, dye degradation, and growth promotion coupled with As resilience in rice seedlings consolidate the sustainable agricultural potential of BioSeNPs. However, their impact on soil ecology and interaction with other contaminants requires further study.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103575"},"PeriodicalIF":3.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immobilization of fungal laccase on peanut shell carriers","authors":"M.P. Serbent ,&nbsp;M.D. Rodríguez ,&nbsp;C. Saux ,&nbsp;I. Magario","doi":"10.1016/j.bcab.2025.103581","DOIUrl":"10.1016/j.bcab.2025.103581","url":null,"abstract":"<div><div>Supported enzymes are more stable than free enzymes, can be reused and easily separated from reaction products, which makes them even more interesting. On the other hand, research into alternatives for the use of residual biomass in the context of the circular economy is crucial. The aim of this work was to investigate the use of peanut shells (PS) and its derivative biochar (PSB) as carriers for the immobilization of <em>Phlebia brevispora</em> BAFC 633 fungal laccase (Lac). No significant differences were found between the enzymatic activity of Lac immobilized on PS or PSB using 2,6-dimethoxyphenol as substrate, so only PS was used for further studies. A screening design was performed with the following immobilization parameters: Contact time, Lac concentration, PS concentration and agitation. The analysis of variance showed that the factors with a statistically significant influence on the promoted enzyme activity (U g^-1) were the agitation and the Lac concentration. For optimization, both factors were further investigated, improving the Lac activity on PS to 0.170 ± 0.042 U g⁻¹, which corresponds to a 2.3-fold relative increase in activity yield. The immobilization efficiency was high, but a decrease in activity was observed with increasing enzyme loading. The evaluation of the results using adsorption equilibria and the estimation of the fractional occupancy of the Lac indicate a still low surface occupancy. Considering its low cost, easy preparation and acceptable activity, PS/Lac biocatalysts are promising alternatives for water remediation.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103581"},"PeriodicalIF":3.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial hydrolysis of Camellia seed cake with Bacillus subtilis: Fermentation process optimization and bioactivity assessment of the hydrolysates","authors":"Lingyun Yao ,&nbsp;Qingru Huang ,&nbsp;Huatian Wang ,&nbsp;Tao Feng ,&nbsp;Chuang Yu ,&nbsp;Kun Xie ,&nbsp;Hao Liu ,&nbsp;Shiqing Song ,&nbsp;Li Shao ,&nbsp;Min Sun","doi":"10.1016/j.bcab.2025.103579","DOIUrl":"10.1016/j.bcab.2025.103579","url":null,"abstract":"<div><div>The <em>Camellia</em> seed cake (CSC) is by-product of tea tree (<em>Camellia oleifera</em>) oil that rich in proteins and traditionally discarded or used as fertilizers, which would lead to a serious waste of the high-quality resources. In this work, <em>Bacillus subtilis</em> was employed to the submerged fermentation of CSC for production of potential bioactive peptides. After optimization by single-factor experiments and response surface methodology (RSM), the optimal fermentation conditions revealed to be material-to-liquid ratio of 30 g/L, inoculum volume of 19.0 %, and temperature of 35.4 °C, and the peptide production yield reached 11.68 % after RSM optimization. The amino acid composition analysis of CSC hydrolysates (CSCH) showed that CSCH was mainly composed of Tyr, Cys, Gly and Val, and 94.01 % of the peptides contained in the CSCH had a molecular weight of less than 3 kDa. In addition, the CSCH revealed strong antioxidant and tyrosinase inhibition potential in vitro, and the Lineweaver–Burk analysis indicated that CSCH exhibited a reversible competitive inhibition against the tyrosinase. Peptides were supposed to be major components that contribute to the bioactivity of CSCH due to their high contained concentration. The results demonstrated that CSCH could be developed as a new type of pure natural antioxidant and anti-tyrosinase agents used for health-promoting products, and these findings would provide useful information for better transformation and utilization of the CSC by-products.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103579"},"PeriodicalIF":3.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprotective microorganisms as antifungal and mycotoxin-degrading agents in food animals production","authors":"Alberto Gonçalves Evangelista ,&nbsp;Tiago de Melo Nazareth ,&nbsp;Carlos Luz ,&nbsp;Victor Dopazo ,&nbsp;Ana Moreno ,&nbsp;Mario Riolo ,&nbsp;Giuseppe Meca ,&nbsp;Fernando Bittencourt Luciano","doi":"10.1016/j.bcab.2025.103574","DOIUrl":"10.1016/j.bcab.2025.103574","url":null,"abstract":"<div><div>Global meat production reached 357.39 million tons in 2021, with mycotoxin contamination, particularly zearalenone (ZEA) and fumonisin B1 (FB1), posing a major challenge due to their resistance to conventional preservation methods. These toxins negatively impact animal health, productivity, and food safety, requiring innovative control strategies. Alternative approaches, such as bioprotective microorganisms, may contribute to mycotoxin degradation and fungal control in the animal production chain. This study evaluated 23 bacterial and yeast strains for their ability to degrade ZEA and FB1 and inhibit mycotoxigenic fungi. Four bacterial strains effectively degraded ZEA: <em>Bacillus amyloliquefaciens</em> subsp. <em>plantarum</em> MLB3, <em>Bacillus subtilis</em> MLB2, <em>Bacillus velezensis</em> CL197, and <em>Streptomyces griseus</em> CECT 3276, though none achieved satisfactory FB1 degradation. The bacteria and their metabolites were effective in inhibiting fungal growth in both solid and liquid culture media, and simulated swine and poultry digestion demonstrated complete ZEA degradation, forming low-toxicity conjugates. These results suggest that selected bacterial strains could be applied in animal production to mitigate mycotoxin contamination, reduce fungal proliferation, and enhance food safety. The use of bioprotective bacteria represents a promising biotechnological approach to safeguarding both animal and human health while improving the quality and safety of animal-derived food products.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103574"},"PeriodicalIF":3.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization and molecular docking of xylanase produced by novel species Bacillus aryabhattai isolated from mangrove soil and its biodegradation efficiency","authors":"Jayabalan Jayaprakash ,&nbsp;Devadoss Johnmilton ,&nbsp;Mani Ganesh ,&nbsp;Chandrasekaran Swaminathan ,&nbsp;Paramasivam Sivagurunathan ,&nbsp;Paramanathan Baranidharan ,&nbsp;Antonysamy Lawrance ,&nbsp;Soundararajan Muthukumaran ,&nbsp;Victoria Anand Mary","doi":"10.1016/j.bcab.2025.103582","DOIUrl":"10.1016/j.bcab.2025.103582","url":null,"abstract":"<div><div>Xylanase-producing bacteria play a crucial role in the degradation of lignocellulosic biomass, facilitating the breakdown of xylan into xylooligosaccharides and xylose. This study aimed to isolate and characterize xylanase-producing bacteria from mangrove ecosystems in Cuddalore and Pondicherry, India. A total of 22 bacterial isolates were obtained, out of which five (PMS1, PMS8, PYMW, SBMS4, and SBW1) exhibited significant xylanolytic activity. Molecular characterization using 16S rRNA sequencing identified these isolates as belonging to the <em>Bacillus</em> genus, with <em>Bacillus aryabhattai</em> (PYMW) and <em>Bacillus subtilis</em> (PMS8) demonstrating the highest xylanase activity. Optimization studies revealed that enzyme production peaked at pH 8.0, with maltose and glucose serving as the most effective carbon sources, and yeast extract enhancing enzyme yield (4.1 ± 0.2 U/mL) and (3.2 ± 0.2 U/mL) respectively. The xylanase enzyme was purified using ammonium sulfate precipitation, yielding a molecular weight of 50 kDa as confirmed by SDS-PAGE. Further, immobilization using sodium alginate beads enhanced enzyme stability and reusability, making it a viable candidate for industrial applications. Molecular docking studies provided insights into enzyme-substrate interactions, highlighting key residues involved in xylan degradation. These findings underscore the potential of mangrove-derived <em>Bacillus</em> strains as sustainable sources of xylanase for applications in bioethanol production, paper manufacturing, and waste management. Future research should focus on large-scale production, genetic modifications for enhanced efficiency, and industrial applications of immobilized xylanase.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103582"},"PeriodicalIF":3.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of green synthesized silver nanoparticles using the aqueous extract of Lavandula angustifolia Mill. Against ethanol-induced gastric ulcers in rats","authors":"Maryam Moradi ,&nbsp;Parichehr Hanachi ,&nbsp;Seifollah Bahramikia ,&nbsp;Mansoureh Tavan","doi":"10.1016/j.bcab.2025.103584","DOIUrl":"10.1016/j.bcab.2025.103584","url":null,"abstract":"<div><div><em>Lavandula angustifolia</em> Mill. (Lamiaceae) possess valuable bioactive compounds such as polyphenols and thus this plant is gaining interest to green synthesis of nanoparticles as source of reducing, stabilizing, and capping agents. ‏Therefore, in the present study, aqueous extract of.</div><div><em>L. angustifolia</em> was used for green synthesis of silver nanoparticles (La-AgNPs). The characterizations of La-AgNPs were determined by UV–Vis spectroscopy, FTIR, XRD, SEM, and EDX. Additionally, the effect of La-AgNPs on gastric damage caused by ethanol in desert rats was investigated. Firstly, Wistar rats were pre-treated with La-AgNPs (5 and 20 mg/kg) and famotidine at (50 mg/kg). Then, an oral dose of ethanol was administered to rats to induce gastric ulcers. The results showed that La-AgNPs had optimal size (69.35 nm) and spherical and cubical shapes. Moreover, the consumption of La-AgNPs orally caused a significant decrease in gastric ulcer index and gastric juice volume, as well as an increase in pH. In addition, tissue nitric oxide (NO), superoxide dismutase, catalase, and glutathione levels increased and serum NO, reactive oxygen species, and malondialdehyde levels decreased in rats pre-treated with La-AgNPs and famotidine drug compared to the ethanol group. The histopathological results confirmed the above cases. This study showed that La-AgNPs can be a promising treatment for gastric ulcer disease caused by ethanol consumption.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103584"},"PeriodicalIF":3.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimisation of diesel degradation and growth kinetic modelling by Antarctic Janthinobacterium lividum","authors":"How Swen Yap ,&nbsp;Farah Eryssa Khalid ,&nbsp;Rasidnie Razin Wong ,&nbsp;Peter Convey ,&nbsp;Suriana Sabri ,&nbsp;Khalilah Abdul Khalil ,&nbsp;Azham Zulkharnain ,&nbsp;Faradina Merican ,&nbsp;Hasrizal Shaari ,&nbsp;Siti Aqlima Ahmad","doi":"10.1016/j.bcab.2025.103573","DOIUrl":"10.1016/j.bcab.2025.103573","url":null,"abstract":"<div><div>Fuel spills pose significant pollution threats to Antarctic terrestrial environments. Biological remediation offers a sustainable solution for restoring these polluted sites. Indigenous microorganisms capable of degrading diesel hydrocarbons at low temperatures could have the potential to restore polluted sites in Antarctic regions. This study represents the first comprehensive investigation into the effects of physiochemical conditions on diesel biodegradation and the growth kinetics of <em>Janthinobacterium lividum</em>, a native Antarctic bacterium previously identified for its diesel-degrading and biosurfactant-producing capabilities at low temperatures. Using a one-factor-at-a-time (OFAT) approach followed by response surface methodology (RSM), we optimised key culture conditions (10.00 °C, 0.00 % NaCl, 1.49 g/L NH4Cl, pH 7.05, 5.67 % v/v initial diesel concentration, and 14.10 % v/v inoculum size), achieving a five-fold enhancement in total petroleum hydrocarbon degradation, reaching 20.57 mg/mL. The Aiba-Edwards growth kinetics model mathematically best described the growth kinetics of the species in the presence of a toxic substrate. This study demonstrates statistically guided optimisation strategies for enhancing diesel biodegradation in <em>Janthinobacterium</em> species and demonstrates the bacteria's remarkable ability to tolerate high diesel concentrations even at low temperatures, reinforcing their potential for Antarctic bioremediation applications.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103573"},"PeriodicalIF":3.4,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}