Biocatalysis and agricultural biotechnology最新文献

{"title":"Green synthesis of TiO2 and ZnO nanoparticles using grape pomace extract: characterization and application in cotton fabric","authors":"Carlos Francisco Moreno-Cruz ,&nbsp;Daniel González-Mendoza ,&nbsp;Ulin Antobelli Basilio-Cortes ,&nbsp;Onecimo Grimaldo-Juárez ,&nbsp;Benjamín Valdez-Salas ,&nbsp;Ernesto Beltran-Partida ,&nbsp;Olivia Tzintzun-Camacho","doi":"10.1016/j.bcab.2025.103645","DOIUrl":"10.1016/j.bcab.2025.103645","url":null,"abstract":"<div><div>The textile industry faces significant challenges in water treatment due to chemical dyes used. The agroindustrial waste is an important source of natural dyes and compounds for the green synthesis of nanoparticles (NPs). This study focused on the characterization of TiO₂ and ZnO nanoparticles from pink grape pomace extracts (PGPEs) and their applications in cotton fabrics as colored nanoparticles. Characterization of TiO₂ and ZnO was carried out using an UV–Vis spectrophotometer, and particle sizes were determined using dynamic light scattering. Additionally, the functional groups present in the TiO₂ and ZnO nanoparticles were identified using Fourier-transform infrared spectroscopy (FTIR). Absorption peaks for TiO₂ and ZnO were observed in the range of 270–400 nm. The smallest nanoparticles were obtained for 1 mM pH 6 ZnONPs and 5 mM pH 6 ZnONPs (13.09 ± 6.42 nm and 13.14 ± 7.23 nm, respectively). The values of zeta potential indicated colloidal excellent stability for 5 mM pH 6 TiO₂NPs, and 1 mM pH 6 ZnONPs. The FTIR analysis suggests that phenolic compounds from PGPEs contribute to the synthesis of nanoparticles. Fabrics treated with TiO₂NPs exhibited light brown, and pink tones, while treatments with ZnNPs resulted in grayish-white colors in both cases. Variations in ΔE values indicated clear differences between the untreated cotton fabrics and those treated. In addition, chroma increased as a function of particle size, favoring the UV absorption and color fixation. Therefore, this study reveals the use of TiO₂NPs and ZnONPs from PGPEs as an environmentally friendly alternative to chemical dyes used in the textile industry.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103645"},"PeriodicalIF":3.4,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel GABA-stabilized gold nanoparticles for plant systems: Synthesis, characterization, and unprecedented effects on growth, physiological function, and nutrient efficiency in Lactuca sativa L.","authors":"Pushpanjali Yadav,&nbsp;Mohammad Amir,&nbsp;Abdul Raheem,&nbsp;Shaheen Anjum Khan,&nbsp;Manisha Sharma,&nbsp;Mohammad Israil Ansari","doi":"10.1016/j.bcab.2025.103644","DOIUrl":"10.1016/j.bcab.2025.103644","url":null,"abstract":"<div><div>Conventional methods of nutrient supply for the growth of plants are not very suitable in the field of agriculture. To enrich the field of agricultural sciences in view of enhancing crop productivity, nutrient supply, soil health, sustainable farming practices and human welfare, an advanced technology termed as “Nanotechnology” is widely explored due their amazing potential applications. In this study, we present the synthesis, comprehensive characterization, and agricultural application of GABA-AuNPs. GABA, a plant-derived, eco-friendly molecule, acts as both a reducing and stabilizing agent, offering promising agronomic advantages. The successful synthesis of GABA-AuNPs was confirmed through UV–Vis spectroscopy, dynamic light scattering (DLS), zeta potential analysis, and transmission electron microscopy (TEM), revealing uniformly dispersed nanoparticles with stable colloidal behaviour, optimal size distribution, and requisite negative zeta potential. Moreover, confirmation of functional group interaction and nanoparticle capping was obtained through FTIR spectroscopy, indicating successful binding between GABA and AuNPs. To investigate the chemical state and surface composition of the GABA-AuNPs, X-ray photoelectron spectroscopy (XPS) analysis was conducted. Further, agricultural efficacy of various concentrations (25, 50, 100, 200, 300 and 400 μM) of synthesized GABA-AuNPs was evaluated in <em>Lactuca sativa</em> L. (lettuce), a nutritionally important crop known for its rich content of dietary fibres, phenolics, vitamins, and minerals. Seed priming with 100 μM GABA-AuNPs significantly enhanced germination rates, growth metrics, photosynthetic pigment levels, and antioxidant enzyme activity. Additionally, GABA-AuNP treatment improved mineral nutrient uptake, optimized stomatal function, and reduced oxidative stress, as evidenced by a decrease in MDA content. However, higher concentrations (≥400 μM) exhibited diminishing returns, with potential negative effects on growth and nutrient homeostasis. These findings suggest that GABA-AuNPs offer a novel strategy for promoting plant growth, nutrient assimilation, and stress resilience, though concentration-dependent effects warrant careful consideration for practical agricultural applications.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103644"},"PeriodicalIF":3.4,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell wall degradation by glucanase activity: Trichoderma pleuroticola and Pythium ultimum interaction","authors":"Samira Shahbazi ,&nbsp;Hamed Askari ,&nbsp;Mehrdad Alizadeh","doi":"10.1016/j.bcab.2025.103643","DOIUrl":"10.1016/j.bcab.2025.103643","url":null,"abstract":"<div><div>During mycoparasitism, <em>Trichoderma</em> fungi secrete various cellulases and glucanases. This study aimed to investigate the role of endo-glucanase and exo-glucanase enzymes in the biological control of the soil-borne pathogen <em>Pythium ultimum</em> by <em>Trichoderma</em>. To enhance enzyme production, <em>Trichoderma</em> spores were irradiated with a γ-ray dose of 250 Gy, and the resulting mutants were compared to the wild-type strain. Using proteomics and cellulase enzyme assays, we assessed the antagonistic activity of wild-type and mutant <em>Trichoderma</em> isolates against <em>P. ultimum</em> in dual culture tests. After 48 h of fermentation, cellulase activity in the culture supernatants was measured using different substrates. Extracellular proteins were characterized using SDS-PAGE and two-dimensional electrophoresis to identify the most prominent catalytic components involved in enzyme production. The <em>Trichoderma</em> isolates (wild-type and mutant) were identified as <em>Trichoderma pleuroticola</em> based on the sequence analysis of the ITS1-5.8 S-ITS2 and TEF-1α regions. The mutant isolates, particularly NAS109-M21, demonstrated significantly increased enzyme activity compared to the wild-type. NAS109-M21 exhibited 2.5 times higher exo-glucanase activity and three times higher endoglucanase activity. In dual culture assays, this mutant strain showed a 65 % reduction in <em>P. ultimum</em> growth, which is five times greater than the wild-type. Furthermore, SDS-PAGE and 2D electrophoresis revealed sharper bands for endo-glucanase, exoglucanase, and β-glucosidase in the mutant strain. These findings suggest that gamma irradiation can induce mutations in <em>T. pleuroticola</em>, leading to enhanced enzyme production and improved biocontrol potential against <em>Pythium ultimum</em> in soil.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103643"},"PeriodicalIF":3.4,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycelial vigor, growth, physicochemical and sensory characteristics of Lentinula edodes produced from agroindustrial residues and their anaerobic digestates","authors":"Oscar H. López-Balladares ,&nbsp;David de la Lama-Calvente ,&nbsp;Francisco J. Flores-Flor ,&nbsp;Rafael Borja","doi":"10.1016/j.bcab.2025.103637","DOIUrl":"10.1016/j.bcab.2025.103637","url":null,"abstract":"<div><div>In some Latin American countries, agro-industrial wastes (AIW) are put aside in significant quantities, as it is treated as common garbage without a specific management plan. A viable alternative would be to use AIW as substrates for further valorization processes. In this research, it was proposed to cultivate <em>Lentinula edodes</em>, in substrates prepared from mixtures of AIW such as cocoa husk, coconut shell, sugar cane bagasse and rice husk. In addition, the digestate derived from the anaerobic digestion of a mixture of these AIW was also evaluated to cultivate this fungus. The mycelial vigor of <em>Lentinula edodes</em> was evaluated during in vitro cultivation, seed production and cultivation at the substrate level prepared from AIW. Physical characteristics, growth and physicochemical analysis of the cultivated fungus and a sensory analysis of basidiomas were performed. The highest mycelial vigor was obtained in PDA culture medium, at pH 7.0, AIW extract, incubation at 20 °C and spore inoculum in liquid consistency. Anaerobic digestate produced basidiomas with higher pH, protein, mineral and moisture content (6.7 ± 0.1; 20.7 ± 0.1 %; 4.5 ± 0.3 %; 86.9 ± 0.6 %, respectively), when compared to the trial without anaerobic digestate. The mushrooms produced showed a moderate difference in flavour compared to commercial mushrooms. It is concluded that <em>Lentinula edodes</em> developed very well in the mixture of the aforementioned AIW with a high nutritional and sensorial value.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103637"},"PeriodicalIF":3.4,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of green synthesized iron oxide nanoparticles from Rhododendron arboretum on wheat (Triticum aestivum): Evaluation of morpho-physiological, biochemical and yield attributes","authors":"Atul Singh ,&nbsp;Tanuja Rawat ,&nbsp;Oddem Govardhan ,&nbsp;Chhavi Sharma ,&nbsp;Pushpa Lohani ,&nbsp;Anuj Kumar ,&nbsp;Shabir Hassan ,&nbsp;Hemant Singh ,&nbsp;Sundip Kumar ,&nbsp;Raju Ratan Yadav ,&nbsp;Sneh Gautam","doi":"10.1016/j.bcab.2025.103640","DOIUrl":"10.1016/j.bcab.2025.103640","url":null,"abstract":"<div><div>In the present study, iron oxide nanoparticles (IONPs) were synthesized by the green method using <em>Rhododendron arboreum</em>, and their effect was evaluated on wheat. The synthesized nanoparticles were characterized by a UV–visible spectrophotometer, peak at 240 nm, indicating the formation IONPs. DLS demonstrated the hydrodynamic diameter of 82 nm of synthesized IONPs with 0.2 PDI. FE-SEM and FTIR confirmed the synthesis of IONPs. Further, a pot experiment was carried out to explore the effect of foliar application of various concentrations (50–200 ppm) of IONPs on two wheat genotypes: VL 953 (unfortified) and HD 3086 (iron-fortified). Among the various morpho-physiological parameters, a maximum increase of 65.9 % was recorded in carotenoid content at 150 ppm concentrations of IONPs than the control. The peroxidase activity exhibited the most significant augmentation of 132.3 % compared to the control at a concentration of 100 ppm among various antioxidant enzyme activities. Both morpho-physiological parameters and antioxidant activities were recorded higher in VL 953 genotype than HD 3086. Grain yield was documented as the highest in all yield parameters at 50 ppm in HD 3086 genotype, which was 11.8 % higher than the control. Moreover, grain iron content was increased with an increase in the concentration (50–200 ppm) of IONPs in both genotypes, and the maximum iron content was documented at 200 ppm with 60.9 % increase than the control. The present work revealed the potential of IONPs to significantly enhance the morpho-physiological parameters, promote the production of antioxidant compounds, and improve grain yield and iron content in both genotypes.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103640"},"PeriodicalIF":3.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of enzymatic saccharification of pretreated sugarcane bagasse for enhanced polyhydroxybutyrate (PHB) production by Bacillus megaterium PNCM 1890","authors":"Ryan Christian R. Suplito ,&nbsp;Princess J. Requiso ,&nbsp;Catalino G. Alfafara ,&nbsp;Fidel Rey P. Nayve Jr. ,&nbsp;Jey-R S. Ventura","doi":"10.1016/j.bcab.2025.103634","DOIUrl":"10.1016/j.bcab.2025.103634","url":null,"abstract":"<div><div>Polyhydroxyalkanoates (PHAs) are microbial polyesters that serve as biodegradable and bio-based alternatives to conventional plastics. Using renewable feedstocks, such as lignocellulosic biomass, offers a sustainable approach to PHA production while mitigating petrochemical dependence and plastic waste accumulation. In this study, enzymatic saccharification of sequentially pretreated sugarcane bagasse (SCB) was optimized to maximize hydrolysate yield for polyhydroxybutyrate (PHB) fermentation. Key parameters—solids loading, enzyme loading, and saccharification time—were evaluated for their impact on reducing sugar yield (RSY). A numeric factorial experiment confirmed their significant effects, and response surface methodology with a face-centered central composite design was employed. ANOVA results revealed the significance of the main and quadratic effects of these factors on RSY. Optimization identified ideal conditions as 4.94 % (w/v) solids loading, 102.98 filter paper units (FPU)/g dry biomass enzyme loading, and 70.44 h of saccharification, yielding 0.46 g reducing sugar/g dry biomass. Fermentation of the optimized hydrolysate achieved a maximum PHB concentration of 6.11 g/L after 20 h, with glucose consumption exceeding xylose. These findings provide a foundation for local bioplastic production using sugarcane bagasse.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103634"},"PeriodicalIF":3.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144291462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and functional properties of bioactive peptide fractions derived from shrimp (Pleoticus muelleri) waste hydrolysates","authors":"Clara Liebana ,&nbsp;Nair de los Ángeles Pereira ,&nbsp;Analía Verónica Fernández-Gimenez ,&nbsp;Luis Ignacio Granone ,&nbsp;María Florencia Fangio","doi":"10.1016/j.bcab.2025.103639","DOIUrl":"10.1016/j.bcab.2025.103639","url":null,"abstract":"<div><div>Protein hydrolysates were prepared from shrimp (<em>Pleoticus muelleri</em>) waste through autolysis (Aut) and using 1 % and 2 % (v/v) Alcalase (Alc 1 and Alc 2, respectively). The degree of hydrolysis (DH) and antioxidant activities were assessed via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS^•+) scavenging assays at various hydrolysis times. After 90 min, Aut and Alc 1 were selected for fractionation by ultrafiltration, yielding peptide fractions: F1 (&gt;10 kDa), F2 (3–10 kDa), and F3 (&lt;3 kDa). Functional properties, antioxidant capacities, and lipid peroxidation inhibition in raw fish emulsions were assessed for both whole hydrolysates (WH) and their peptide fractions. Alc 1 achieved a significantly higher DH (42.02 ± 5.50 %) than Aut (28.75 ± 2.34 %). Both hydrolysates and their fractions displayed high solubility (&gt;65.49 ± 2.07 %) across a wide pH range. The F1 fraction exhibited the strongest DPPH scavenging activity for both hydrolysates, with Aut's F1 reaching 445.90 ± 19.52 mg AEAC/100 g dry weight and Alc 1's F1 achieving 461.10 ± 15.81 mg AEAC/100 g dry weight. Aut's F3 fraction had the highest ABTS^•+ scavenging capacity (723.66 ± 36.58 mg AEAC/100 g dry weight), while Alc 1's WH exhibited superior activity (776.20 ± 52.55 mg AEAC/100 g dry weight). Overall, Aut's F1 fraction demonstrated the most favorable antioxidant and functional properties, while Alc 1's F3 fraction showed superior solubility and lipid peroxidation inhibition. These findings underscore the potential of shrimp (<em>P. muelleri</em>) waste for producing functional hydrolysates and bioactive peptides with diverse applications.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103639"},"PeriodicalIF":3.4,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144291461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable extraction of cannabidiol using alkaline leaching coupled with green deep eutectic solvents","authors":"Qurat Ul Ain Haidery ,&nbsp;Yue Shen ,&nbsp;Jingping Niu ,&nbsp;Chenyue Yan ,&nbsp;Ziwen Xu ,&nbsp;Bo Han ,&nbsp;Jingyu Wang ,&nbsp;Yanlin Wang ,&nbsp;Xingyuan Ma ,&nbsp;Wenyun Zheng","doi":"10.1016/j.bcab.2025.103636","DOIUrl":"10.1016/j.bcab.2025.103636","url":null,"abstract":"<div><div>Cannabidiol (CBD), a non-psychoactive phytocannabinoid, has garnered attention owing to its paramount medicinal potential and leisure purposes. However, to meet the growing practical demand, obtaining high-purity CBD without toxic organic solvents and suitability for large-scale processing of raw materials with simple and low-cost extraction processes has become a major practical issue. This study proposes an innovative and viable technique for extracting CBD by conjugating deep eutectic solvents (DES) (ethanol and lactic acid 2:1) with alkaline leaching (NaOH). All process parameters, including leaching duration (time), pH, and extraction temperature, were optimized meticulously using response surface methodology (RSM). The CBD-rich part of the plant was identified by QE-LC/MS analysis. The outcomes were validated by repeated experiments with the highest yield of 14.8 mg/g. The adopted method produced 83.48 % pure CBD, which was 32 % more effective than ethanol leaching, as proven by HPLC analysis. Fourier transform infrared spectroscopy (FTIR) provided insight into the composition of the extracted CBD, confirming its integrity and chemical structure. Degradation kinetic analysis provided insight about the storage temperatures. The results indicated that the extracting technique was simply reliable and environmentally safe, proven by environmental impact assessment indicators, and can be utilized on an industrial scale for economically extracting bioactive compounds from various plant sources.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103636"},"PeriodicalIF":3.4,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzyme-assisted recovery of phenolic compounds from broccoli waste with antioxidant and antihyperglycemic activities evaluated through in vitro and molecular docking studies","authors":"Carlos N. Cano-Gonzalez ,&nbsp;Rocio Yaneli Aguirre-Loredo ,&nbsp;Juan Alberto Ascacio Valdes ,&nbsp;Cristobal N. Aguilar González ,&nbsp;Lluvia de Abril Alexandra Soriano-Melgar","doi":"10.1016/j.bcab.2025.103632","DOIUrl":"10.1016/j.bcab.2025.103632","url":null,"abstract":"<div><div>The zero-waste strategy encompasses several Sustainable Development Goals, focusing on using and managing resources to produce value-added compounds. Broccoli waste represents a valuable and underutilized source of phenolic compounds, renowned for their diverse bioactive properties. This study employed an innovative enzyme-assisted extraction (EAE) method as a green and efficient alternative for obtaining these bioactive compounds without compromising their composition and functionality. The objective of this research was to harness sustainable technology to extract phenolic compounds from broccoli waste, maximizing their potential bioactivities. The EAE, specifically the commercial enzymatic preparation (Pectinase with polygalacturonase activity of 100 U g⁻¹), exhibited a high extraction efficiency of phenolic compounds (14.53 mg GAE g⁻¹) and flavonoids (7.63 mg QE g⁻¹), with an antioxidant activity of 95.33 %. Flavonoid glycosides demonstrated molecular docking interactions (≤−8.0 kcal mol⁻¹) with the catalytic amino acids of α-amylase and DPP-4. This provides a scheme to indicate the source and extraction method to obtain compounds of importance as therapeutic alternatives in chronic degenerative diseases. The findings highlight the potential application of these phenolic compounds as functional food additives, capable of scavenging free radicals and regulating postprandial blood glucose levels.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103632"},"PeriodicalIF":3.4,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of α-tocopherol biomolecules using various cultivation strategies of diverse microalgal species: Purification, characterization and assessment of antioxidant properties","authors":"Udaypal,&nbsp;Rahul Kumar Goswami,&nbsp;Pradeep Verma","doi":"10.1016/j.bcab.2025.103635","DOIUrl":"10.1016/j.bcab.2025.103635","url":null,"abstract":"<div><div>Tocopherols are valuable biomolecules with antioxidant and anticancer properties, synthesized by photosynthetic organisms. Their presence in microalgae remains underexplored due to a lack of specific extraction and purification techniques. This study examines the potential of three microalgae species<em>, Tetraselmis indica</em> (TS), <em>Chlorella vulgaris</em> (CV), and <em>Picochlorum</em> sp. (PC) through heterotrophic, phototrophic, and mixotrophic cultivation methods, focusing on their influence on biomass production and α-tocopherol yield, an important antioxidant. The results demonstrated maximum biomass production by TS, CV, and PC, reached 1.92 ± 0.08, 1.97 ± 0.13, and 1.99 ± 0.02 g/L, respectively, under mixotrophic conditions with supplementation of 7.5 g/L sodium acetate for TS and PC, and 1 g/L glucose for CV. The yield of α-tocopherol biomolecules obtained from TS, PC, and CV was 2.82 ± 0.12, 1.57 ± 0.06, and 0.51 ± 0.01 mg/L, respectively. Microalgal α-tocopherol was purified using column chromatography and used for antioxidant activity, e.g., DPPH, ABTS, and OH^●scavenging activity. The DPPH and ABTS radical scavenging activities of purified α-tocopherol from TS, PC, and CV biomass were 79.63 ± 1.22, 76.42 ± 2.42, and 78.17 ± 2.01 %, and 30.20 ± 1.52, 28.86 ± 0.59, and 29.66 ± 1.72 %, respectively. The characterization of purified α-tocopherol was performed using FTIR, LC-HRMS, and ¹H and ¹³C NMR spectroscopy. The production of high-purity α-tocopherol from microalgae is beneficial for industrial and pharmaceutical applications and supports the concept of a bio-circular economy.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103635"},"PeriodicalIF":3.4,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144261774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}