Biocatalysis and agricultural biotechnology最新文献

{"title":"Eco-friendly degradation of methyl orange using Ralstonia pickettii","authors":"Asranudin ,&nbsp;Adi Setyo Purnomo ,&nbsp;Eirene Grace Fransina ,&nbsp;Holilah ,&nbsp;Agus Wedi Pratama ,&nbsp;Alya awinatul Rohmah ,&nbsp;Silvia Abdi Pratama ,&nbsp;Khanom Simarani ,&nbsp;Setyo Budi Kurniawan ,&nbsp;Ulfa Dwiyanti ,&nbsp;Muhammad Iqbal Hidayat","doi":"10.1016/j.bcab.2025.103658","DOIUrl":"10.1016/j.bcab.2025.103658","url":null,"abstract":"<div><div>The widespread problem of dye pollution, with hazardous chemicals like methyl orange (MO) contaminating water sources, poses a significant threat to both the environment and human health. This study explored a promising and environmentally friendly solution: biodegradation of MO using the bacterium <em>Ralstonia pickettii</em>. The results demonstrated that <em>R. pickettii</em> effectively decolorized MO (100–200 mg/L) by 98 % under static conditions. The optimal conditions for decolorization were found to be a pH of 7 and a temperature of 40 °C. The decolorization of MO was maximized when the growth medium contained substrates like lactose broth, ammonium sulfate, urea, yeast extract, and gelatin. The culture exhibited significant decolorization capacity for 48 h. The activities of enzymes like laccase, NADH-DCIP reductase, and azoreductase suggest their involvement in breaking down MO structure. Metabolites formed after decolorization, including N, N-dimethylaniline (m/z 121), 4-amino-2,6-dihydroxybenzoate (m/z 169), and (2Z,4E)-5-amino-2,5-dihydroxypenta-2,4-dienoate (m/z 144), were characterized using FTIR and LC-TOF/MS. Phytotoxicity test showed the MO was toxic and metabolites obtained after its decolorization were nontoxic for the mungbean plant.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103658"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144517499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic synthesis of Albizia lucidior mediated magnetite nanoparticles (Fe3O4 NPs): Therapeutic applications and photocatalytic activity","authors":"Vrushali Manoj Hadkar ,&nbsp;Nayan Kumar Sishu ,&nbsp;Swathy Prakash Jayabharathi ,&nbsp;Arunagiri Sharmila ,&nbsp;Chinnadurai Immanuel Selvaraj","doi":"10.1016/j.bcab.2025.103676","DOIUrl":"10.1016/j.bcab.2025.103676","url":null,"abstract":"<div><div>This study presents the biogenic synthesis of magnetite (Fe₃O₄) nanoparticles using <em>Albizia lucidior</em> ethanolic leaf extract, a novel green approach leveraging the plant's rich phytochemical profile, including phenols (157.37 ± 1.69 mg GAE/g), flavonoids (224.65 ± 1.74 mg QE/g), and terpenoids (231.08 ± 1.90 mg LNE/g) for the first time. Characterization techniques of nanoparticles, including XRD, FTIR, UV–Vis, AFM, FESEM and EDX were performed. FESEM and XRD confirmed the formation of spherical NPs with a crystallite size of 12.28 nm. The NPs exhibited significant antioxidant activity (DPPH scavenging: 64.55 ± 0.49 %; metal chelation: 73.77 ± 0.6 %) and demonstrated cytotoxic effects against HeLa (45.11 ± 1.4 %) and MCF-7 (53.88 ± 0.6 %) cell lines. Photocatalytic studies revealed 60.32 % degradation of sunset yellow dye by NPs under sunlight. The phytotoxicity assays indicated a growth-stimulating effect on <em>Vigna radiata</em> seeds. These significant findings underscore the potential of <em>A. lucidior</em> mediated Fe₃O₄ NPs as innovative nano-formulation, offering mechanistic insights into therapeutic actions, while also serving as effective tools for environmental protection through effective pollutant degradation.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103676"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144548499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of glutamic acid production capacity of Stenotrophomonas sp. strain CG2 isolated from soil","authors":"Cihat Guner ,&nbsp;Ertan Ermis ,&nbsp;Kubra Ozkan Guner","doi":"10.1016/j.bcab.2025.103665","DOIUrl":"10.1016/j.bcab.2025.103665","url":null,"abstract":"<div><div>Glutamic acid is a widely used amino acid in the food and pharmaceutical industries due to its role as a flavor enhancer and a metabolic precursor. This study aimed to identify glutamic acid-producing bacterial strains from soil samples collected across different regions. Among 262 isolates screened, <em>Stenotrophomonas</em> sp. strain CG2 exhibited the highest production capacity and was identified through 16S rRNA sequencing. Fermentation parameters including pH, temperature, incubation time, and agitation speed were optimized using the Plackett–Burman design, leading to a maximum yield of 3.76 ± 0.65 g/L under optimized conditions (pH 7.0, 30 °C, 200 rpm, 84 h), compared to 2.72 g/L in unoptimized TSB medium. The produced glutamic acid was purified using ion-exchange resin, yielding a recovery efficiency of 53.48 ± 3.28 %, and its identity was confirmed by FT-IR, RAMAN, and LC-MS/MS analysis.</div><div>This study is among the first to systematically explore Stenotrophomonas spp. for glutamic acid biosynthesis under optimized fermentation conditions. The results provide insight into the strain's specific responses to nutrient composition, revealing its potential for future biotechnological applications. By expanding the microbial landscape of amino acid producers, this work offers a foundation for using CG2 in sustainable bioproduction processes, particularly those leveraging food or agro-industrial waste streams.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103665"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of bacterial biomass production using soybean molasses-based medium as a potential component for bean crop inoculant development","authors":"Ana Cristina Silva da Natividade,&nbsp;Rui dos Santos Ferreira Filho,&nbsp;Janaína Fernandes de Medeiros Burkert,&nbsp;Carlos André Veiga Burkert","doi":"10.1016/j.bcab.2025.103668","DOIUrl":"10.1016/j.bcab.2025.103668","url":null,"abstract":"<div><div>This study aimed to optimize bacterial biomass production using a soybean molasses-based medium in shaken flask cultures as a potential component for bean crop inoculant development. <em>Bradyrhizobium yuanmingense</em> SEMIA 6462, <em>Bradyrhizobium pachyrhizi</em> SEMIA 6464, <em>Rhizobium tropici</em> SEMIA 4077, and <em>Rhizobium freirei</em> SEMIA 4080 were evaluated. <em>B. yuanmingense</em> SEMIA 6462 presented the highest biomass production, which was 5.48 g L⁻¹ after 72 h of cultivation. For this microorganism, a Plackett-Burman (PB) design with ten variables followed by a 2⁴ Central Composite Rotatable Design (CCRD) were proposed to optimize biomass production. The variables with the greatest influence were soybean molasses, sodium chloride, calcium chloride dihydrate, and yeast extract concentrations, with the pH adjusted to 7, while dibasic and monobasic potassium phosphates, magnesium sulfate heptahydrate, manganese chloride heptahydrate, and Tween 80 were removed from the medium. The best conditions established by an empirical model and validated by experimental assays were 100 g L⁻¹ soybean molasses, 0.8 g L⁻¹ yeast extract, 0.45 g L⁻¹ sodium chloride, and 0.3 g L⁻¹ dihydrate calcium chloride. Under these conditions, biomass production reached 38.6 g L⁻¹, representing a 604.93 % increase from the initial value (5.48 g L⁻¹) while also reducing the medium cost per kilogram of biomass by 70 %. The results demonstrate the efficacy of cultivation conditions in increasing rhizobia biomass production, highlighting the potential of soybean molasses as an alternative and sustainable substrate to produce microbial inoculants.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103668"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144522504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green-synthesized nanoparticles: recent trends and innovations towards sustainable agriculture","authors":"Ameni Ben Zineb ,&nbsp;Muhammad Fasih Khalid ,&nbsp;Mahmoud Gargouri ,&nbsp;Juha M. Alatalo ,&nbsp;Talaat Ahmed","doi":"10.1016/j.bcab.2025.103667","DOIUrl":"10.1016/j.bcab.2025.103667","url":null,"abstract":"<div><div>Environmental challenges posed by conventional chemical methods in agriculture have sparked a revolution in green nanotechnology to provide eco-friendly solutions. In this regard, green-based nanoparticles (GB-NPs) offer significant economic and ecological benefits while reducing the environmental impact. This review dives deep into the synthesis and characterization of GB-NPs, highlighting their key synthesis factors, limitations, and their role in plant stress tolerance. Notably, this review goes beyond previous reports by providing an in-depth analysis of recent research employing GB-NPs for mitigating plant pests and diseases. A Sankey diagram was used to illustrate the studies and quality of evidence on GB-NPs outcomes for the mitigation of phytopathogens during the last ten years, providing valuable insights into their application in mitigating biotic stress. Additionally, to bridge the gap between research and real-world application, a conceptual framework is proposed to facilitate the large-scale adoption of GB-NPs in sustainable agriculture, laying the groundwork for future research and practical implementation.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103667"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144522503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Augmented elimination of cadmium and mercury by Cytobacillus firmus and Paenibacillus massiliensis isolated from heavy metal contaminated soil samples","authors":"Ankita Ghosh,&nbsp;Moumita Chakraborty,&nbsp;Diksha Sah,&nbsp;J.P.N. Rai","doi":"10.1016/j.bcab.2025.103669","DOIUrl":"10.1016/j.bcab.2025.103669","url":null,"abstract":"<div><div>Cadmium and mercury, often classified as heavy metals or toxic elements, are prevalent in the environment due to industrial activities and pollution, posing risks to ecosystems and human health. The current research utilized the metal tolerance abilities of extremely resilient microorganisms, both individually and as part of a consortium, to address the complex issue of heavy metal pollution remediation. These bacterial strains were isolated from contaminated soil and subjected to screening under different treatment conditions. The examination and screening investigations have unveiled an isolated strain as Cd resistant bacteria viz. BS4 (<em>Cytobacillus firmus</em>) which have shown Cd removal up to 82 % and Hg tolerant bacterial isolate namely, BS10 (<em>Paenibacillus massiliensis</em>) with Hg removal efficiency of more than 63 %. Furthermore, the engineered microbial consortium (BS4 + BS10) has proven its capability to enhance the removal of Cd and Hg from soil samples. Extensive validation considered various factors such as pH, temperature, initial concentrations of Cd and Hg, and size of the inoculum, establishing the consortium's biosorption capacity. Structural alterations induced by microbial treatment were evidenced through FESEM and FTIR analysis. The metal-binding proficiency exhibited by this bacterial consortium underscores its potential as an efficient biosorbent for addressing heavy metal contamination.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103669"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Larvicidal activity, oxidative stress, and enzymatic alterations induced by essential oil from Piper cyrtopodon (Miq.) C.DC (Piperaceae) on Aedes aegypti (Culicidae)","authors":"Maria L.L. da Costa ,&nbsp;André C. de Oliveira ,&nbsp;Vinícius B. Ribeiro ,&nbsp;Suelen C. Lima ,&nbsp;Felipe M.A. da Silva ,&nbsp;Sergio M. Nunomura ,&nbsp;Rita de C. S. Nunomura ,&nbsp;Rosemary A. Roque ,&nbsp;Tatiane P. de Souza","doi":"10.1016/j.bcab.2025.103661","DOIUrl":"10.1016/j.bcab.2025.103661","url":null,"abstract":"<div><div>Dengue, Zika, and chikungunya are mosquito-borne diseases transmitted by <em>Aedes aegypti</em>, with over 13 million dengue cases, 431.223 chikungunya cases, and 44.242 Zika cases reported in the Americas in 2024. The resistance of <em>Ae. aegypti</em> to synthetic insecticides has driven the search for botanical alternatives, such as essential oils (EOs) from <em>Piper</em> species. This study evaluated the EO from <em>Piper cyrtopodon</em> and its main compounds as larvicides, investigating their mechanism of action. The EO, extracted by hydrodistillation (yield: 1.4 ± 0.2 %), contained germacrene D (57.46 %), cadina-1(10),4-diene (10.57 %), and <em>δ</em>-elemene (8.0 %). It showed significant larvicidal activity against <em>Ae. aegypti</em>, with LC₅₀ values ranging from 20.78 to 146.61 μg/mL over 24 and 48 h. Cadina-1(10),4-diene was the most active compound (<em>p</em> &lt; 0.0001). Biochemical analyses revealed oxidative stress in larvae, with increased reactive oxygen species production (11 ± 2 to 26 ± 2 μmol H₂O₂/g), lipid peroxidation (0.54 ± 0.06 to 2.8 ± 0.22 ηmol MDA/g), and protein oxidation (1.62 ± 0.04 to 4.54 ± 0.51 nmol/mg protein) compared to controls (<em>p</em> &lt; 0.0001). Antioxidant enzymes superoxide dismutase (10.33 ± 3 to 28.33 ± 6 mU/mg protein), catalase (1.04 ± 0.09 to 7.08 ± 2.42 mmol H₂O₂/min/mg protein), and glutathione peroxidase (5.40 ± 1.37 to 14.67 ± 1.52 mmol NADPH/min/mL) were altered, suggesting redox imbalance as a toxicity mechanism. These findings highlight the potential of the EO from <em>P. cyrtopodon</em> as a natural alternative for <em>Ae. aegypti</em> control.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103661"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144534281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zeatin–auxin synergy and growing media optimization enhanced micropropagation, acclimatization, and antioxidant properties of ‘Bentong’ ginger (Zingiber officinale Roscoe)","authors":"Nisar Ahmad Zahid ,&nbsp;Hawa Z.E. Jaafar ,&nbsp;Mansor Hakiman","doi":"10.1016/j.bcab.2025.103653","DOIUrl":"10.1016/j.bcab.2025.103653","url":null,"abstract":"<div><div>‘Bentong’ ginger (<em>Zingiber officinale</em> Roscoe) is a medicinally and economically important ginger cultivar in Malaysia. However, its conventional propagation via rhizomes is limited by low multiplication rates and a high risk of disease transmission. This study aimed to develop an efficient micropropagation protocol by optimizing the synergistic application of zeatin and auxins. Murashige and Skoog (MS) medium supplemented with 10 μM zeatin and 2.5 μM 1-naphthaleneacetic acid (NAA) significantly enhanced shoot multiplication (6.72 shoots per explant) and overall <em>in vitro</em> shoot development. Furthermore, the effects of different growing media on <em>ex vitro</em> acclimatization were evaluated. Plantlets grown in a 1:1:1 mixture of soil, coco peat, and vermiculite exhibited the highest survival rate (94.79 %) and superior vegetative and physiological performance, followed closely by a mixture of soil, peat moss, and vermiculite (93.75 % survival). Phytochemical analyses revealed no significant differences in total polyphenols content or antioxidant activities, assessed via 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging and iron-chelating assays, between rhizomes of micropropagated and conventionally propagated plants. These findings highlight the potential of zeatin–auxin synergy and optimized acclimatization media to efficiently produce disease-free, true-to-type ‘Bentong’ ginger planting materials.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103653"},"PeriodicalIF":3.4,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144501420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural characterization, functional analysis and computational annotation of a metagenome-derived Glucoamylase enzyme: Effect of temperature, pH, metal Ions, and surfactants on enzyme activity","authors":"Atif Khurshid Wani ,&nbsp;Alaa S. Alhegaili ,&nbsp;Faisal Imam ,&nbsp;Chirag Chopra ,&nbsp;Juliana Heloisa Pinê Américo-Pinheiro ,&nbsp;Farida Rahayu ,&nbsp;Aniswatul Khamidah ,&nbsp;Gatut Wahyu Anggoro Susanto ,&nbsp;Runik Dyah Purwaningrahayu ,&nbsp;Mudasir A. Dar ,&nbsp;Reena Singh","doi":"10.1016/j.bcab.2025.103662","DOIUrl":"10.1016/j.bcab.2025.103662","url":null,"abstract":"<div><div>Glucoamylase (GluAmy) holds significant industrial relevance, particularly in starch processing industries, owing to its ability to efficiently hydrolyze complex carbohydrates into glucose under diverse environmental conditions. In this study, a thermostable and alkalophilic GluAmy gene, 510 bp in length, was amplified from a hot spring metagenome. The gene was initially cloned into the pJET 1.2 vector and transformed into <em>Escherichia coli</em> DH5α, followed by heterologous expression using the pET28a vector in <em>E. coli</em> BL21 (DE3) cells. Purification via Ni-His affinity chromatography yielded GluAmy (19.2 kDa), which was biochemically characterized for activity and stability across a wide pH range (3.0–12.0) and temperatures (10°C–110 °C). The enzyme's activity was influenced by metal ions (Mn²⁺, Mg²⁺, Ca²⁺, Zn²⁺, Fe²⁺, Na⁺, Co²⁺, Cu²⁺, Ni²⁺) at 5–10 mM concentrations, as well as surfactants (Tween-20, Tween-80, Triton X-100, SDS) at 5–10 %. GluAmy demonstrated optimal activity at 80 °C and pH 9.0. Co²⁺ and Ca²⁺ enhanced activity by 115.2 % and 105.6 %, respectively, whereas Tween-20 reduced activity to 56.3 %. The purified enzyme exhibited the highest specific activity against starch (12.94 U/mg), followed by dextrin (11.67 U/mg) at 1 % substrate concentration. Computational analysis revealed a protein structure predominantly composed of random coils (53.25 %), contributing to its thermal stability. These findings underscore the potential of GluAmy as a robust biocatalyst for industrial applications, particularly in processes requiring high temperature and alkaline conditions.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103662"},"PeriodicalIF":3.4,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis of silver nanoparticles using Bougainvillea flower extract: Antibacterial, antibiofilm and cytotoxicity studies","authors":"Mohammad Azam Ansari ,&nbsp;Mohammad N. Alomary","doi":"10.1016/j.bcab.2025.103663","DOIUrl":"10.1016/j.bcab.2025.103663","url":null,"abstract":"<div><div>This study investigated a sustainable approach for synthesizing silver nanoparticles (AgNPs) utilizing an extract from the flower of <em>Bougainvillea glabra</em> plant. The successful synthesis of AgNPs was confirmed by various biophysical techniques: UV–Vis spectroscopy revealed a prominent peak (432 nm), whilst FT-IR and XRD investigations confirmed their chemical composition and crystalline structure. TEM investigations indicated that the AgNPs measured around an average of 25.05 ± 0.87 nm and exhibited a spherical morphology. MIC values against gram-positive and gram-negative ranged from 32 to 128 μg/mL. The XTT assay shows that AgNPs at 500 μg/mL proficiently inhibits bacterial biofilm by 96 %. Light and electron microscopy investigations have indicated that AgNPs dramatically restrict their colonization and adhesion. SEM and TEM show that the cell surface exhibited irregularities, roughness, distortion, and an impaired state, characterized by a defective cellular membrane and its wall, signifying an impairment of membrane integrity that subsequently leads to cell death. Furthermore, the AgNPs exhibited a regulated toxic effect on breast cancer cells, inducing apoptosis while maintaining biocompatibility and sparing healthy cells. The promising results suggest that this eco-friendly way of making AgNPs could lead to new methods for delivering drugs to fight cancer and treat infections associated to biofilms.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103663"},"PeriodicalIF":3.4,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144492143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}