Biocatalysis and agricultural biotechnology最新文献

{"title":"Exploring the functional and probiotic potential of bacteriocin-producing lactobacilli from Algerian Kaddid","authors":"Kamel Boubakri ,&nbsp;Constanza Melian ,&nbsp;Pablo G. Cataldo ,&nbsp;Elvira M. Hebert ,&nbsp;Tayeb Idoui ,&nbsp;Graciela Vignolo","doi":"10.1016/j.bcab.2025.103597","DOIUrl":"10.1016/j.bcab.2025.103597","url":null,"abstract":"<div><div>Research on lactobacilli from traditional food niches have received increasing attention for their beneficial properties. In this study, selected bacteriocin-producing lactobacilli strains previously isolated and characterized from laboratory-prepared <em>Kaddid</em> were used. The lactobacilli were screened <em>in vitro</em> for their probiotic potential including inhibition of <em>Listeria (L.) monocytogenes</em> FBUNT biofilm formation, antioxidant capacity and γ-aminobutyric acid (GABA) production. When probiotic features were investigated, surface characterization of bacteriocinogenic lactobacilli revealed most of them as hydrophilic, however <em>Latilactobacillus</em> (<em>Lat.</em>) <em>sakei</em> subsp. <em>sakei</em> K26 exhibited strong auto-aggregative and hydrophobic character with a positive Pearson (<em>r</em>) correlation between the two characteristics. In addition, <em>Lactiplantibacillus (Lpb.) paraplantarum</em> K18 was the most resistant to assayed low pHs and bile salts being able to withstand gastrointestinal tract (GIT) conditions. Variable biofilm formation patterns were displayed by adhesion in polystyrene plates depending on the modified MRS media composition, higher production for <em>Lat. sakei</em> subsp. <em>sakei</em> K26 and <em>Lpb. paraplantarum</em> K18 was found. When bacteriocin-like inhibitory substances were tested against <em>L. monocytogenes</em> biofilm, <em>Lat. sakei</em> subsp<em>. sakei</em> K26, <em>Lat. sakei</em> K25 and <em>Lpb. paraplantarum</em> K18 were the most efficient at reduced the pathogen in correlation with their ability to produce bacteriocins and biosurfactants. Variable free radical scavenging activity (DPPH<strong>˙</strong> and HO<strong>˙</strong>) and anti-lipid peroxidation was found in lactobacilli strains, while GABA production assay showed <em>Lpb. paraplantarum</em> K18 to reach 18.63 mM with a conversion rate of ∼6.9 % after incubation at 30 °C for 15 days. On these bases and together with previous results, the beneficial effect of <em>Lpb. paraplantarum</em> K18 support its potential to be used as functional/probiotic starter culture for the development of new and safety fermented meat products.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103597"},"PeriodicalIF":3.4,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143906849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved eumelanin production, phenolic content, flavonoid content, and antioxidant activity by Streptomyces lasalocidi NTB 42 following copper sulfate supplementation","authors":"Muhammad Asril ,&nbsp;Rika Indri Astuti ,&nbsp;Iman Rusmana ,&nbsp;Aris Tri Wahyudi","doi":"10.1016/j.bcab.2025.103602","DOIUrl":"10.1016/j.bcab.2025.103602","url":null,"abstract":"<div><div>The demand for natural pigments, including microbial melanin, is rapidly increasing in the pharmaceutical industry. Eumelanin, a black pigment produced by <em>Streptomyces lasalocidi</em> NTB 42, exhibits high antioxidant activity. This pigment can be produced in large quantities using copper sulfate (CuSO₄.5H₂O). This study aimed to evaluate the effect of CuSO₄.5H₂O on various parameters related to eumelanin production in <em>S. lasalocidi</em> NTB 42 and antioxidant activity. The addition of 0.04 % CuSO₄.5H₂O to the production medium significantly enhanced tyrosinase activity by 4.52-fold. Additionally, it resulted in a 46.75-fold increase in eumelanin production (3.74 g/L), a 1.83-fold increase in eumelanin concentration (875 mg/g eumelanin dry weight), a 1.44-fold increase in total phenolic content (TPC) (127.76 mg GAE/g eumelanin), a 0.89-fold increase in the 2,2-diphenyl-1-picryl-hydrazyl scavenging assay (IC₅₀) (66.75 μg/mL), and a 7.68-fold increase in ferric reducing antioxidant power (875.95 mg AAE/g eumelanin) (p &lt; 0.05). Furthermore, the addition of 0.03 % CuSO₄.5H₂O resulted in a 1.30-fold increase in total flavonoid content (TFC) (76.93 mg QE/g eumelanin) and a 0.328-fold increase in total antioxidant capacity (191.41 mg AAE/g eumelanin) (p &lt; 0.05). The obtained eumelanin, which is characterized by UV–VIS and FT-IR analysis, has the same character as the previously found eumelanin. These findings suggest that CuSO4.5H₂O concentrations between 0.03 % and 0.04 % significantly enhance eumelanin production, levels of major chemical compounds, and antioxidant activity in <em>S. lasalocidi</em> NTB 42. This study underscores the potential for increased eumelanin production and antioxidant activity of <em>S. lasalocidi</em> NTB 42 to meet the demands of pharmaceutical applications as an antioxidant agent.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103602"},"PeriodicalIF":3.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143885965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Media optimisation for enhanced β-glucosidase production in Komagataella pastoris","authors":"T. van Coller,&nbsp;S.H. Rose,&nbsp;O.D. Biko,&nbsp;T. Jansen","doi":"10.1016/j.bcab.2025.103603","DOIUrl":"10.1016/j.bcab.2025.103603","url":null,"abstract":"<div><div>Cost-effective protein production requires maximal target protein expression levels in affordable cultivation media. The <em>Komagataella pastoris</em> pGAPZαA expression vector was adapted for expression of the <em>Phanerochaete chrysosporium</em> β-glucosidase reporter gene in <em>Komagataella pastoris</em> and <em>Saccharomyces cerevisiae</em> resulting in volumetric activities of 0.26 and 0.52 U/mL, respectively. The <em>K. pastoris</em>[pccbgl1] strain was subsequently cultivated in modified BMGY with different nitrogen source substitutions for YNB. Soy whey yielded an extracellular β-glucosidase activity of 0.60 U/mL, 2.07-fold greater than obtained with standard BMGY. Hereafter, novel simple media (SM) combinations were designed, which resulted in a 2.71-fold increase in β-glucosidase activity relative to BMGY in shake flask cultivations. The simple media compared well with the routinely used basal salt medium (BSM) in 1-L batch fermentation studies. The BSM, SMb (SM with brewer's spent yeast) and SMs (SM with soy whey) resulted in volumetric activities of 2.26, 2.79 and 1.29 U/mL and productivities of 84, 120 and 49 U/g DCW, respectively after 30 h of aerobic cultivation [2.8, 4.0 and 1.63 U/g DCW/h]. The novel SMb compares well with the BSM and has potential use for the economical production of recombinant proteins on an industrial scale. A key consideration when using the current waste streams as a nitrogen source is the presence of additional proteins, which may impact the downstream purification process when a highly pure product is required.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103603"},"PeriodicalIF":3.4,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143901987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory properties of a β-glucan-rich bioflour of Bactris gasipaes shells colonized by Lentinula edodes","authors":"Thailise Gonçalves Timm ,&nbsp;Thaynã Gonçalves Timm ,&nbsp;Mayra Alice Corrêa Pitz ,&nbsp;Cristiane Vieira Helm ,&nbsp;Michele Debiasi Alberton ,&nbsp;Lorena Benathar Ballod Tavares ,&nbsp;Isabel Daufenback Machado","doi":"10.1016/j.bcab.2025.103594","DOIUrl":"10.1016/j.bcab.2025.103594","url":null,"abstract":"<div><div><em>Lentinula edodes</em> β-glucans have shown potential in reducing lung injury caused by inflammation associated with severe acute respiratory syndrome (SARS). This study aimed to investigate the immunomodulatory properties related to lung inflammation, both <em>in vitro</em> and <em>in vivo</em>, of a high-β-glucan content bioflour derived from peach palm (<em>Bactris gasipaes</em>) shells colonized by <em>Lentinula edodes</em> (BPL). Neutrophils, L929, and A549 cells were treated (<em>in vitro</em>) with different doses (1, 10, 100 μg/mL) of BPL extracts (EBPL). The cells were subjected to cell viability assays, oxidative stress evaluation, and NO₂ quantification. Male Swiss mice were divided into five groups (<em>in vivo</em>). Lung inflammation was induced via intranasal instillation of LPS (4 mg/kg), and the mice were treated orally with BPL. A control group received a vehicle or dexamethasone (0.5 mg/kg). Bronchoalveolar lavage (BAL) was collected to quantify total leukocytes, the mouse-to-lung weight ratio was calculated, and histological analysis was performed. Treatment with EBPL (<em>in vitro</em>) did not induce toxicity in cell lines and NO₂ production decreased in neutrophils treated with the extracts; however, it did not reduce oxidative stress in A549 cells. A reduction in leukocytes was observed (<em>in vivo</em>) in BAL [11.43 × 10⁵ (control), 9 × 10⁵ (3 mg/kg), 3.33 × 10⁵ (30 mg/kg), 1.43 × 10⁵ (300 mg/kg) leukocytes/mL), histological infiltration, and a lower lung/mouse weight ratio [91.27 (control), 78.20 (3 mg/kg), 65.37 (30 mg/kg), 71.08 (300 mg/kg)]. This study highlights the nutraceutical potential of a β-glucan-rich food ingredient as an adjunct therapy for treating lung inflammation through dietary means, promoting human health and nutrition.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103594"},"PeriodicalIF":3.4,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"6-Ishwarone: A novel eco-friendly botanical biocide inducing oxidative stress and disrupting defense enzyme activities in Aedes aegypti Linnaeus, 1762 and Anopheles darlingi Root, 1926 (Culicidae)","authors":"André C. de Oliveira ,&nbsp;Ingrity S. Costa Sá ,&nbsp;Felipe M.A. da Silva ,&nbsp;Suelen C. Lima ,&nbsp;Maria Luiza L. da Costa ,&nbsp;Sergio M. Nunomura ,&nbsp;Rosemary A. Roque ,&nbsp;Rita C.S. Nunomura","doi":"10.1016/j.bcab.2025.103596","DOIUrl":"10.1016/j.bcab.2025.103596","url":null,"abstract":"<div><div><em>Aedes aegypti</em> and <em>Anopheles darlingi</em> are vectors of dengue and malaria in Brazil. Currently, a alternative for their control is the use of essential oils (EOs) and their isolated compounds, which are environmentally friendly agents for larval management due to their low toxicity to non-target animals. Thus, in the study, the EO from <em>Piper alatipetiolatum</em> and its main compound were isolated and evaluated against larvae these mosquitoes, focusing on the mechanism of action. The lethal effect these products were also evaluated against non-target animals. The structure of the 6-ishwarone, a new sesquiterpene, was established using 1D and 2D NMR and HRAPCIMS data. 6-Ishwarone and the EO exhibited larvicidal activity (LC₅₀ from 25.03 to 42.58 μg/mL), accompanied by an overproduction of reactive oxygen and nitrogen species (36.67 ± 7 to 49.33 ± 1 %), an increase in both glutathione S-transferase (36.00 ± 8 to 80.67 ± 7 μmol/min/mg protein) and catalase activities (0.31 ± 0 to 0.50 ± 0 μmol of H₂O₂ consumed/min/mg of protein), followed by a decrease in acetylcholinesterase activity (36.00 ± 8 to 25.33 ± 1 μmol/min/mg protein) and a reduction in thiol content (30.00 ± 3 to 13.33 ± 3 μmol/mg protein). Furthermore, 6-ishwarone and the EO (LC₅₀ from 415.25 to 739.29 μg/mL), were less toxic to <em>A. bouvieri</em>, <em>T. splendens</em>, <em>G. affinis</em>, and <em>D. indicus</em> compared with α-cypermethrin (LC₅₀ from 0.23 to 0.35 μg/mL). These findings underscore the potential of these products for developing new mosquito control strategies.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103596"},"PeriodicalIF":3.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First derivative of Gompertz equation: Identification of substrate fractions in psychrophilic anaerobic digestion","authors":"Mario Esparza-Soto,&nbsp;Sergio Alcaraz-Ibarra,&nbsp;Mercedes Lucero-Chávez,&nbsp;María del Carmen Jiménez-Moleón,&nbsp;Miroslava de los Angeles Mier-Quiroga,&nbsp;Cheikh Fall","doi":"10.1016/j.bcab.2025.103595","DOIUrl":"10.1016/j.bcab.2025.103595","url":null,"abstract":"<div><div>The biochemical methane potential (BMP) test is widely used to model methane production from organic wastes during anaerobic digestion (AD), often with the Modified Gompertz (MG) kinetic model. Further analysis of the MG model can provide critical insights into the hydrolysis and methanogenic stages of AD. While BMP tests are traditionally conducted at mesophilic temperatures, conducting them at the inoculum source temperature has been recommended. This study evaluated the BMP of waste activated sludge (<em>WAS</em>), organic kitchen waste (<em>OKW</em>), and two co-digestion ratios (Co-D1: 65/35 % <em>OKW/WAS</em>; Co-D2: 35/65 % <em>OKW/WAS</em>) at a psychrophilic temperature of 20 °C. A novel spreadsheet-based approach utilizing the first derivative of the Gompertz equation identified substrate fractions, kinetic constants and AD stages insights. Co-digestion prevented media acidification and enhanced methane yields, with Co-D2 producing 261.0 mLCH₄/g-VSadd—significantly higher than <em>WAS</em> (192.8) and <em>OKW</em> (81.2). These yields are comparable to those from similar substrates under mesophilic conditions (35–37 °C), underscoring the energy potential of psychrophilic co-digestion. The kinetic constants achieved coefficients of determination exceeding 0.90. The first derivative method identified two substrate fractions: readily and slowly biodegradable organic matter. This research provides new insights into anaerobic microbial dynamics and substrate degradation pathways, demonstrating the effectiveness of psychrophilic co-digestion for methane production.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103595"},"PeriodicalIF":3.4,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of black soldier fly (Hermetia illucens L.) growth and nutrition on food waste and bovine blood mixture as a feedstock","authors":"Agus Dana Permana ,&nbsp;Irtyafari Almira Haswara ,&nbsp;Khalilan Lambangsari ,&nbsp;Brahmani Dewa Bajra","doi":"10.1016/j.bcab.2025.103590","DOIUrl":"10.1016/j.bcab.2025.103590","url":null,"abstract":"<div><div>Bovine blood is considered one of the major biowastes in a slaughterhouse that contains high nutrition that can be recycled and utilized, especially protein. However, the current treatment to address the issue of bovine blood was confronted with a pressing issue: environmental pollution and cost effectivity. Therefore, it is necessary to convert bovine blood into a high-value form using a bioreactor. Black soldier fly offers a wide range of bioconverting solutions that have the potential to convert bovine blood into a high-value and more acceptable protein source. However, little is known about the growth and nutritional content of black soldier fly larvae fed with bovine blood as their feedstock constituent. Here we show the life span, survival rate, growth rate, waste reduction index, and feed conversion efficiency of black soldier fly larvae fed with bovine blood as feedstock. In a controlled environment, 20 % bovine blood treatment achieved the highest growth rate and protein content. The study's results suggest that black soldier fly larvae, when combined with food waste, can bioconvert bovine blood, resulting in a significant final larval weight and high protein content. The resulting prepupal and pupae hold potential as nutritionally valuable feed for poultry and fish.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103590"},"PeriodicalIF":3.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143854492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The newly-isolated Levilactobacillus brevis LABC170 and Limosilactobacillus fermentum LABC37 with purine nucleosides degradation activity show probiotic efficacy in prevention and treatment of hyperuricemia in mice","authors":"Mokhammad Khoiron Ferdiansyah ,&nbsp;Seung Hyeon Ji ,&nbsp;Myeong Seong Cha ,&nbsp;Yong Hwi Kwon ,&nbsp;Ga Yeong Kim ,&nbsp;Beomseok Park ,&nbsp;Gaddapara Manasa ,&nbsp;Kwang-Pyo Kim","doi":"10.1016/j.bcab.2025.103592","DOIUrl":"10.1016/j.bcab.2025.103592","url":null,"abstract":"<div><div>Purine nucleosides are recognized as key contributors to hyperuricemia. In this study, we isolated 81 Lactic Acid Bacteria (LAB) from various sources and evaluated their purine nucleosidase (PNase) activity. Among them, <em>Levilactobacillus brevis</em> LABC170 exhibited complete degradation of guanosine and inosine (100 %), while <em>Limosilactobacillus fermentum</em> LABC37 showed high degradation rates (92 ± 1.72 % guanosine, 88 ± 4.6 % inosine). Both strains demonstrated probiotic characteristics, including acid resistance and adhesion to Caco-2 cells. In hyperuricemia-induced mice, LAB administration significantly reduced blood uric acid (hyperuricemia group: 2.7 ± 0.5 mg/dL vs. LAB-treated groups: 1.8 ± 0.2 to 2.2 ± 0.3 mg/dL, p &lt; 0.05). Notably, <em>L. fermentum</em> LABC37, despite lower PNase activity than <em>L. brevis</em> LABC170, was more effective in reducing uric acid when administered before hyperuricemia induction, suggesting probiotic effects beyond PNase activity. These findings highlight the strain-specific variations in PNase activity and the potential application of newly isolated LAB strains in hyperuricemia prevention and treatment.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103592"},"PeriodicalIF":3.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytoconstituents and bioactivities of Cassia fistula leaf and stem bark: Antioxidant capacity and α-glucosidase inhibition via molecular docking and in vitro approaches","authors":"Francis Xavier ,&nbsp;Muhammad Hanafi ,&nbsp;Yesi Desmiaty ,&nbsp;Puspa DN. Lotulung ,&nbsp;Minarti Minarti ,&nbsp;Lia Meilawati ,&nbsp;Nina Artanti","doi":"10.1016/j.bcab.2025.103589","DOIUrl":"10.1016/j.bcab.2025.103589","url":null,"abstract":"<div><div><em>Cassia fistula</em> is a traditional medicinal plant especially used in Ayuverdic and Traditional Chinese Medicine (TCM) for various diseases. This study aims to evaluate the antioxidant and α-glucosidase inhibition activities of extracts and fractions from <em>C. fistula</em> leaves and stem bark. <em>C. fistula</em> leaves and stem barks were extracted using methanol, and then stem barks extract (BME) further fractionated with hexane (BHF), ethyl acetate (BEAF), and methanol (BMF) due to its higher α-glucosidase inhibition than leaves extract (LME). Extracts and fractions were analyzed TPC, TFC, antioxidant capacity (DPPH, FRAP, and ABTS), α-glucosidase inhibition, LC-HRMS, and molecular docking of identified compounds. BME exhibited higher TPC and TFC values at 38.23 mgGAE/g and 98.41 mgQE/g compared to LME at 3.12 mgGAE/g and 4.46 mgQE/g, while stem bark fractions contain TPC values ranging from 8.44 to 39.44 mgGAE/g and TFC from 33.62 to 121.86 mgQE/g. BME exhibited higher antioxidant capacity than LME, while BEAF exhibited the highest antioxidant capacity compared to other fractions. Similarly, BME also exhibited higher α-glucosidase inhibition than LME, with IC₅₀ values of 2.81 μg/mL and 60.48 μg/mL, respectively, while BME fractions α-glucosidase inhibition ranging from 5.86 to 12.15 μg/mL. LC-HRMS identified 32 unique compounds consisting of phenolics, flavonoids, steroids, terpenoids, anthraquinones, and coumarins. Among the identified compounds, quercetin 3-O-rhamnoside-7-O-glucoside demonstrated the highest binding affinity to α-glucosidase (PDB ID: 3A4A) trough molecular docking. These findings suggest BME possesses higher antioxidant capacity and α-glucosidase than LME, while BEAF showed the highest compared to other fractions.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103589"},"PeriodicalIF":3.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing sustainable sweet grass (Lippia dulcis L.) productivity and essential oil content using sunn hemp (Crotalaria juncea L.) as green manure","authors":"Rafaela Malheiros Salomon ,&nbsp;Rafael Marlon Alves de Assis ,&nbsp;João Pedro Miranda Rocha ,&nbsp;Jeremias José Ferreira Leite ,&nbsp;Thayane Ferreira Miranda ,&nbsp;Jandeilson Pereira dos Santos ,&nbsp;Lissa Izabel Ferreira de Andrade ,&nbsp;José Eduardo Brasil Pereira Pinto ,&nbsp;Suzan Kelly vilela Bertolucci","doi":"10.1016/j.bcab.2025.103593","DOIUrl":"10.1016/j.bcab.2025.103593","url":null,"abstract":"<div><div>The application of green manure can sustain biological diversity while maintaining high soil fertility. Therefore, using green manure is a crucial practice for minimizing the environmental and ecological impact of sustainable agricultural development. <em>Lippia dulcis</em> is a medicinal plant used to treat coughs, colds, and bronchitis, as well as having potential antidiabetic and sweetening properties. The aim of the current study is to assess the influence of <em>Crotalaria juncea</em> green manure on <em>Lippia dulcis</em> vegetative growth and essential oil accumulation, as well as on total phenolic compounds and verbascoside levels. The experiment was conducted in the field, based on six treatments, namely: soil and sand substrate (negative control); chemical fertilizer (positive control); as well as 150; 300; 450; and 600 g of green manure in pot 10 L. Dry weights, leaf analysis, photosynthetic pigment levels, total phenolics, total flavonoids, verbascoside, as well as essential oil content, yield and chemical components were assessed. The treatment comprising 600 g of green manure recorded the most significant results for plant biomass. On the other hand, doses of 450 g and 600 g of green manure yielded the best results in terms of essential oil content and yield. In contrast, the control treatment recorded the best results for verbascoside and total flavonoids. Chemical fertilizer and 300 g green manure were the treatments that recorded the most efficient results for phenolic compounds. Using green manure instead of conventional fertilization had a positive influence on the attributes analyzed in the species <em>Lippia dulcis</em>, and it is a cheaper and more sustainable strategy for growing medicinal plants.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103593"},"PeriodicalIF":3.4,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143854490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}