Biocatalysis and agricultural biotechnology最新文献

{"title":"Evaluation of ash derived from conversion of contaminated Miscanthus × giganteus biomass as a soil amendment: impacts on soil parameters and physiological characteristics of Zea mays L.","authors":"Andriy Herts ,&nbsp;Abdulmannan Rouhani ,&nbsp;Oleksandr Kononchuk ,&nbsp;Viktor Markiv ,&nbsp;Oksana Horyn ,&nbsp;Volodymyr Khomenchuk ,&nbsp;Vitalii Stadnik ,&nbsp;Pavlo Shapoval ,&nbsp;Valentina Pidlisnyuk","doi":"10.1016/j.bcab.2025.103786","DOIUrl":"10.1016/j.bcab.2025.103786","url":null,"abstract":"<div><div>Biomass ash (BA) is a sustainable soil amendment with the potential to reduce dependency on chemical fertilizers, enhance soil quality, and improve crop productivity, in accordance with the principles of the circular economy. This study evaluated the effects of <em>Miscanthus × giganteus</em> (<em>M×g</em>) ash, derived from biomass grown on trace element (TE) contaminated land in France, applied at rates ranging from 0 % to 7 %, on soil properties, nutrient dynamics, and the physiological responses of the test plant, <em>Zea mays</em> L. The study results demonstrated that BA application significantly increased soil pH (from 6.2 to 7.5) and electrical conductivity (from 0.42 to 2.14 dS m⁻¹), indicating enhanced buffering capacity and ion-exchange potential. The soil organic carbon content reached a maximum of 3.8 % with a 5 % ash application rate, suggesting its potential for carbon sequestration. Nutrient analysis revealed a substantial increase in K concentration, reaching 51,691 mg kg⁻¹ in the plant leaves at the 7 % BA level, while Mg and P concentrations decreased to 826 and 567 mg kg⁻¹, respectively, at the same dose. A higher ash application rate (7 %) caused physiological stress and nutrient imbalances, thereby limiting the growth of <em>Z. mays</em> plants. These findings highlight the dual effects of BA as a soil amendment: while lower application rates (1 % and 3 %) improve soil fertility and plant growth, higher doses (5 % and 7 %) pose potential risks. Given the large-scale availability of <em>M×g</em> BA from the thermal conversion of biomass cultivated on TE-phytoremediated sites, these results support its practical use in sustainable agriculture and land reclamation, provided that application rates are carefully optimized to balance benefits and risks.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103786"},"PeriodicalIF":3.8,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glandular prickles in Rosa hybrida accumulate specific sesquiterpenes implicated in defense against aphid pests","authors":"Aarzoo Dhiman ,&nbsp;Anand Mishra ,&nbsp;Rajveer Singh ,&nbsp;Rimpy Diman ,&nbsp;Mohit Kumar Swarnkar ,&nbsp;Poonam Kumari ,&nbsp;Vivek Dogra","doi":"10.1016/j.bcab.2025.103785","DOIUrl":"10.1016/j.bcab.2025.103785","url":null,"abstract":"<div><div>Rose, a plant of immense ornamental value and economic importance, is often infested by herbivory and insectivory. To overcome this, rose plants have evolved epidermal appendages, namely prickles, which accumulate distinct secondary metabolites that protect the rose plants against pests. The hard, edgy, and sharp appendages found mainly on stems and leaves are called non-glandular prickles (NGPs). Interestingly, rose plants bear distinct soft and glandular appendages, which are present beneath the flower bud and petioles, referred to as glandular prickle (GPs). The NGPs, being sharp structures and accumulating secondary metabolites, are implicated in protecting against herbivores; the relevance of GPs, however, is unclear. Given their presence beneath flower bud, we speculated that GPs might be involved in defense against insect pests. Investigation of morphology, diversity, and development of GPs in <em>Rosa hybrida</em> cv. ‘Superstar Supreme (SS)’ in open field conditions revealed that GPs are epidermal/cortical outgrowths with a multicellular stalk and a glandular head with a cavity that seems to release exudates. The targeted biochemical assessment revealed an accumulation (and release in exudates) of secondary metabolites, including lignin, flavonoids, and terpenes, that were likely involved in defense against insect pests. In agreement, red-colored aphids were found trapped in the GPs beneath the floral bud, reinforcing the notion that GPs or exudates likely contain insect attractants and insecticidal metabolites. Acetylcholine esterase inhibition assay indicated the insecticidal potential of GPs. Untargeted metabolite analysis revealed accumulation and release of volatile sesquiterpene hydrocarbons, such as β-cis-caryophyllene, γ-muurolene and β-Copaene, that likely deter aphids by either direct insecticidal effect or attracting their predators to feed on them. These findings provided significant insights that GPs in rose accumulate specific secondary metabolites and are involved in defense against aphid pests, protecting the newly grown floral bud.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103785"},"PeriodicalIF":3.8,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of xylo-oligosaccharides from corn cobs using a xylanase-rich cocktail produced by Fusarium sp. E.A1.3.1 in free and alginate-immobilized forms, and in vitro fermentation with Lacticaseibacillus rhamnosus Lyofast LRB and Lactiplantibacillus plantarum LMG P-21021L","authors":"Irene Andressa ,&nbsp;Glauce Kelly Silva do Nascimento ,&nbsp;Tatiane Monteiro dos Santos ,&nbsp;Daniela de Oliveira Teotônio ,&nbsp;Ana Flávia Coelho Pacheco ,&nbsp;Paulo Henrique Costa Paiva ,&nbsp;Paulo de Souza Costa Sobrinho ,&nbsp;Larissa de Oliveira Ferreira Rocha ,&nbsp;Bruno Ricardo de Castro Leite Júnior ,&nbsp;Vivian Machado Benassi ,&nbsp;Marcio Schmiele","doi":"10.1016/j.bcab.2025.103781","DOIUrl":"10.1016/j.bcab.2025.103781","url":null,"abstract":"<div><div>Xylo-oligosaccharides (XOS) have garnered attention due to their prebiotic properties and associated health benefits. However, the challenge of high production costs persists in industrial-scale manufacturing. This study aimed to optimize xylan extraction parameters from purple pericarp corn cobs and to produce XOS using a xylanase-rich cocktail from <em>Fusarium</em> sp. E.A1.3.1 (EnCoF). Its performance was compared to that of a commercial xylanase, both in free (CoXyA) and immobilized forms on alginate beads. Optimal conditions were determined as 2 M NaOH at 60 °C, achieving a desirability index of 89.9 %, validated by mathematical modeling. Xylans extracted under these conditions were devoid of monomeric carbohydrates (xylose or glucose). Soluble CoXyA yielded higher XOS quantities compared to EnCoF. Conversely, immobilized EnCoF exhibited superior XOS yields over CoXyA. Soluble enzymes demonstrated enhanced substrate accessibility, thereby facilitating efficient XOS production. Meanwhile, EnCoF immobilized on alginate beads exhibited robust stability for reuse, retaining approximately 70 % activity across 10 cycles. In vitro fermentation assays indicated that XOS derived from NaOH-pretreated substrate served as a carbon source for <em>L. rhamnosus</em> Lyofast LRB and <em>L. plantarum</em> LMG P-21021L, yielding results similar to commercial fructo-oligosaccharides. In conclusion, the production of XOS from corn cobs utilizing <em>Fusarium</em> sp. E.A1.3.1 represents a promising strategy to mitigate production costs, suitable for incorporation into symbiotic foods with probiotics.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103781"},"PeriodicalIF":3.8,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-efficacy of Hedyotis sithiravaraiensis callus extract: A stepwise evaluation through phytochemical profiling, antioxidant activity, molecular docking, and cytotoxicity","authors":"Nagaraj Nallakaruppan,&nbsp;Rajasekaran Chandrasekaran,&nbsp;Kalaivani Thiagarajan","doi":"10.1016/j.bcab.2025.103783","DOIUrl":"10.1016/j.bcab.2025.103783","url":null,"abstract":"<div><div>This study investigates the bioactive potential of <em>Hedyotis sithiravaraiensis</em> S. Muruganandam, Devanath., S. Ravikumar &amp; D. Naras. Sp. nov. callus extract through Phytochemical Profiling, Antioxidant activity, Molecular Docking, ADME analysis, and Cytotoxicity in A549 lung cancer cells. The highest levels of total phenolic content (45.96 ± 0.80 mg GAE/g) and total flavonoid content (45.28 ± 0.53 mg QE/g) were found in 75 % acidified methanol. The DPPH scavenging activity (IC₅₀ = 617.81 ± 0.54 μg/mL) and the ABTS⁺ radical cation scavenging activity (IC₅₀ = 554.41 ± 0.25 μg/mL) both demonstrated that this extract exhibits significant antioxidant activity. Seventeen bioactive components were found in the <em>H. sithiravaraiensis</em> callus extract by GC-MS analysis. Absorption, distribution, metabolism, and excretion studies of 17 compounds also demonstrated favorable drug-like properties and pharmacokinetic characteristics. These bioactive compounds demonstrated significant binding affinities to the KRAS G12C and Bcl-2 proteins, as shown in molecular docking experiments. These affinities were comparable to those of common standard drugs, such as Adagrasib and Obatoclax. The callus extract showed 31.45 ± 0.04 % cytotoxicity in A549 cells at the highest dose, indicating selective cytotoxicity toward lung cancer cells. These findings highlight <em>H. sithiravaraiensis</em> callus extract as a promising anticancer source targeting the MAPK pathway and demonstrate the value of integrated phytochemical and biological analyses in drug discovery and conservation of medicinal plants.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103783"},"PeriodicalIF":3.8,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulatory effects of phytoestrogens, fresh soy milk, and annexin A1 on cell cycle regulation and apoptotic pathways in human leukemic cells and peripheral blood mononuclear cells","authors":"Qalidah Mohamad Ali ,&nbsp;Ahmad Khusairi Azemi ,&nbsp;Siti Hawa Nordin ,&nbsp;Sagir Mustapha ,&nbsp;Muhammad Luqman Nordin","doi":"10.1016/j.bcab.2025.103784","DOIUrl":"10.1016/j.bcab.2025.103784","url":null,"abstract":"<div><div>Phytoestrogens, plant-derived compounds with estrogen-like activity, along with fresh soy milk and Annexin A1, an endogenous protein, exhibit notable anti-cancer properties. This study investigated their effects on apoptosis and cell cycle regulation in human leukemic cell lines (K562, Jurkat, U937) and normal peripheral blood mononuclear cells (PBMCs). Coumestrol, genistein, and Annexin A1 induced statistically significant effects (<em>p</em> &lt; 0.05), primarily causing G2/M phase arrest, followed by G0/G1 arrest, with a modest (∼3–5 %) increase in S-phase cells. In K562 cells, arrest correlated with upregulation of cyclins E1 and A2, and downregulation of cyclin B1, while cyclin D1 showed an inverse pattern. In Jurkat and U937 cells, cyclins D1, E1, A2, and B1 were consistently downregulated. Cell cycle arrest was regulated by p21 independently of p53 in all treated cell lines. Caspase activation patterns varied: caspases-9 and -8 were activated in K562 and U937 cells, while caspase-3 activation occurred only in genistein-treated Jurkat cells. Bcl-2 was upregulated in most treatments, except in coumestrol-treated K562 and genistein-treated Jurkat cells. PBMCs showed S and G2/M arrest without apoptosis or caspase activation. These findings demonstrate the statistically supported (<em>p</em> &lt; 0.05) anti-cancer potential of these agents and suggest their promise as alternative leukemia treatments by modulating distinct apoptotic and cell cycle-regulatory pathways.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103784"},"PeriodicalIF":3.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotypic and probiotic characterisation of lactobacilli strains isolated from traditional Algerian products","authors":"Amina Chadli ,&nbsp;Bouasria Benbouziane ,&nbsp;Véronique Robert ,&nbsp;Philippe Langella","doi":"10.1016/j.bcab.2025.103782","DOIUrl":"10.1016/j.bcab.2025.103782","url":null,"abstract":"<div><div>Twelve bacterial strains isolated from two different traditional Algerian products, cow butter and fermented wheat, were genetically identified using PCR and 16S rRNA sequencing. The growth kinetics of these strains were studied with two different carbon sources, lactose and glucose, each at a concentration of 2 %, alongside evaluations of their probiotic properties and safety. Genotypic identification revealed the presence of three species: <em>Levilactobacillus brevis</em> (4 strains), <em>Lactiplantibacillus plantarum</em> (2 strains), and <em>Lacticaseibacillus paracasei</em> (6 strains). <em>Lp. plantarum</em> and <em>L. paracasei</em> strains showed robust growth on both carbon sources, with similar performance, while <em>L. brevis</em> strains exhibited reduced growth in MRS broth containing lactose compared to MRS broth with glucose. Additionally, all strains demonstrated excellent tolerance to simulated gastric conditions and bile salts, with survival rates above 90 %. However, the <em>L. brevis</em> 64 strain was the most affected by bile salts compared to the other strains, showing a significant decrease in survival rate. Safety evaluations revealed no DNase or β-hemolytic activity, and the strains were generally sensitive to most antibiotics tested. These strains, particularly <em>Lp. plantarum</em> and <em>L. paracasei</em>, isolated from lesser-studied Algerian fermented foods, exhibit promising in vitro probiotic traits and contribute to diversifying available functional microbial resources.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103782"},"PeriodicalIF":3.8,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel galloylated catechin-menthofuran conjugate derived from persimmon tannin with potential anti-ulcer activity","authors":"Lanzhen Ban,&nbsp;Yajie Zhang,&nbsp;Yunfei Huang,&nbsp;Xia Pi,&nbsp;Jiawei Bao,&nbsp;Chunmei Li","doi":"10.1016/j.bcab.2025.103780","DOIUrl":"10.1016/j.bcab.2025.103780","url":null,"abstract":"<div><div>A new method for acid-catalyzed degradation of Persimmon tannin (PT) was established using menthofuran as a nucleophile. Four new flavan-3-ol conjugates were obtained by reacting menthofuran with PT at 60 °C in 0.2M HCl-ethanol solution for 90 min by single factor experiment, with the yield of the main degradation products epigallocatechin gallate-menthofuran (EGCG-MF) and epicatechin gallate-menthofuran (ECG-MF) being 246 mg/g and 77 mg/g, respectively. Animal experiments proved that, compared to the untreated mice, the two main degradation products showed equivalent anti-ulcer activity as positive drug cimetidine, with inhibition rates of 55.32 % and 53.90 % for EGCG-MF and ECG-MF, respectively. H&amp;E staining and PAS staining showed that the two substances had a protective effect on the gastric mucosa. They exerted the anti-ulcer activity by enhancing the expression of tight junction proteins Occludin and Zonula occludens-1 (ZO-1). The recovery rates of the two products for the two proteins were 73.89 %, 80.23 % and 68.72 %,88.44 %, respectively. In addition, by measuring the levels of oxidative stress markers such as MDA, SOD, GSH, as well as inflammatory factors TNF-α, IL-6, IL-10 in mice, it was found that two degradation products could treat gastric ulcers by reducing the levels of oxidative stress and inflammation. This work provided new application ideas for the application of proanthocyanidins in functional foods and medical fields.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103780"},"PeriodicalIF":3.8,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accelerating wheat growth using phosphorus and calcium nano-fertilizers (P-NF and Ca-NF) in a speed breeding chamber compared to conventional Hoagland solution","authors":"Minhas Elahi,&nbsp;Khadeja Saleem,&nbsp;Kapeel Kumar,&nbsp;Zainab bibi,&nbsp;Aysha Hayat,&nbsp;Umar Masood Quraishi","doi":"10.1016/j.bcab.2025.103773","DOIUrl":"10.1016/j.bcab.2025.103773","url":null,"abstract":"<div><div>The current rate of improvement in several major crops is insufficient to meet future demand. This study explores the integration of speed breeding (SB) with phosphorus nano-fertilizer (P-NF) and calcium nano-fertilizer (Ca-NF) to accelerate wheat growth and enhance nutrient uptake. Two concentrations of P-NF and Ca-NF (50 ppm and 100 ppm) were tested in hydroponic and pot experiments, alongside the conventional Hoagland solution, in a SB chamber. The nano-fertilizers were characterized using UV–visible spectroscopy (P-NF peaks at 239 nm, Ca-NF 239.2 and 261 nm), XRD (P-NF 46.06 nm and Ca-NF 25 nm), FTIR, EDS, and SEM, confirming their cubic (P-NF) and spherical (Ca-NF) structure. Results showed that P-NF and Ca-NF significantly improved plant height, grain yield, and biological yield by 5.53 %, 51 %, and 28.66 %, respectively. Days to tillering and maturity were reduced by 5.15 % and 7.08 % showing successful acceleration of the wheat growth cycle. P-NF and Ca-NF also increased antioxidant activity, with superoxide dismutase (SOD) and peroxidase (POD) levels reduced by up to 58.29 % and 44 %, respectively, compared to the control. Nutritional analysis revealed higher protein (42.42 %), lipid (32.61 %), total sugar (30.42 %), and starch (30.19 %) content in grains. Phosphorus uptake increased by 182.44 % and calcium uptake by 222.03 % in pot-grown plants. This study successfully achieved its objective of reducing the wheat growth cycle but also significantly improved yield and nutrient content, offering a sustainable solution for faster and healthier wheat production.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103773"},"PeriodicalIF":3.8,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of chemical and biological pretreatments on biobutanol production from Pennisetum purpureum × Pennisetum typhoideum by semi-simultaneous saccharification and fermentation","authors":"Anuchit Sonwai ,&nbsp;Patiroop Pholchan ,&nbsp;Nakorn Tippayawong","doi":"10.1016/j.bcab.2025.103774","DOIUrl":"10.1016/j.bcab.2025.103774","url":null,"abstract":"<div><div>The conversion of biomass to biofuel is a key strategy for sustainable energy production. Effective pretreatment of lignocellulosic biomass is critical to improving its bioconversion efficiency. Optimum times and temperatures for chemical giant Juncao grass (GJG) pretreatment with sodium hydroxide (NaOH) and N-Methylmorpholine N-oxide (NMMO) were systematically determined. Under the suitable pretreatment conditions, higher percentage of cellulose was remained in NaOH-pretreated GJG (at 81.7 °C, 60.0 min) compared to that pretreated by NMMO (at 138.3 °C, 10.3 min), whereas GJG's properties changed slightly in anaerobic digestion (AD)-pretreated GJG. After the semi-simultaneous saccharification and fermentation (sSSF) process, the highest glucose concentration of 48.3 g L⁻¹ was observed from NaOH-pretreated GJG within the first 24 h and the highest butanol concentration of 8.95 g L⁻¹ was detected after fermentation with <em>Clostridium acetobutylicum</em> ATCC 824 for 96 h. While both chemical pretreatments produced satisfactory acetone-butanol-ethanol (ABE) yields, GJG pretreated with AD was inferior. A suitable conversion sequence of GJG was suggested for butanol followed by methane productions as products provided higher total energy yields with lesser operational complexities. Efficient ABE fermentation of the high-lignin GJG could be achieved using the uncomplicated NaOH treatment with sSSF, which was technically and economically advantageous.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103774"},"PeriodicalIF":3.8,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variation in secondary metabolites of Daphne mucronata Royle (Thymelaeaceae) by climatic factors and ontogenetic stages","authors":"Maryam Alavi Bougar ,&nbsp;Mohammad Jamal Saharkhiz ,&nbsp;Shahram Azadi Bougar ,&nbsp;Atena Eslami-Farouji","doi":"10.1016/j.bcab.2025.103779","DOIUrl":"10.1016/j.bcab.2025.103779","url":null,"abstract":"<div><div>This study investigated the effects of specific climatic factors longitude, latitude, altitude, temperature, and precipitation on the secondary metabolites present in different organs of <em>Daphne mucronata</em> Royle across nine regions in Iran. Iran's diverse physiography gives rise to a wide spectrum of climatic conditions, ranging from arid and hyper-arid deserts on the central plateau to humid subtropical regions along the Caspian coast and cold, mountainous climates in the Alborz and Zagros ranges. This significant climatic variation makes the study of its influence on medicinal plants paramount. Secondary metabolites in various plant organs exhibited variable responses to the climatic factors. The highest phenolic content (1780.51 ± 0.23 mg/100 g DW) was recorded in leaves from the Shekarak region, whereas fruits from Dalin showed the lowest content (557.39 ± 0.7 mg/100 g DW). Total flavonoid content was highest in leaves from Margun (218.3 ± 0.57 mg/100 g DW) and lowest in fruits from Aspas (0.076 ± 0.02 mg/100 g DW). The highest levels of flavones and flavonols were found in leaves from Dalin (29.42 ± 1.03 mg/100 g DW) and Dejkord (25.06 ± 1.36 mg/100 g DW), with no significant difference between these two regions; fruits from Dalin exhibited the lowest content (3.005 ± 1.71 mg/100 g DW). Antioxidant activity was highest and consistent in flowers and fruits across all regions (95.23 ± 0.36 %), but it was lowest in leaves from Kakan (81.37 ± 1.12 %). Gallic acid concentration reached its maximum in leaves from Dalin (2350.87 mg/100 g DW) and its minimum in fruits from Margun (630.23 mg/100 g DW); leaves consistently contained higher gallic acid levels than flowers or fruits. Furthermore, total flavonoid content in flowers decreased with increasing precipitation but increased in fruits. Phenolic compounds in flowers decreased with altitude but increased with temperature.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103779"},"PeriodicalIF":3.8,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}