Biocatalysis and agricultural biotechnology最新文献

{"title":"Engineering a reusable yeast whole-cell biocatalyst via surface display of Burkholderia gladioli lipase BSR","authors":"Zhu Jing,&nbsp;Lin Jiafang,&nbsp;Huang Hanrui,&nbsp;Qin Jinling,&nbsp;Zuo Xiaoqiong,&nbsp;Wang Qingyan","doi":"10.1016/j.bcab.2026.104287","DOIUrl":"10.1016/j.bcab.2026.104287","url":null,"abstract":"<div><div>Yeast surface display is an effective strategy for constructing whole-cell biocatalysts by anchoring enzymes on the cell surface, thereby integrating enzyme expression, immobilization, and catalysis within a single microbial host. In this study, an alkaline and solvent-tolerant lipase (BSR) from <em>Burkholderia gladioli</em> Bsp-1 was displayed on the surface of <em>Saccharomyces cerevisiae</em> using an Aga2-based yeast surface display system. Flow cytometric analysis confirmed the surface localization of BSR on the engineered yeast cells. The engineered yeast whole-cell catalyst achieved a maximum catalytic activity of 79.43 U·g^-1 dry cell weight after 36 h of induction. The displayed enzyme retained the characteristic catalytic properties of purified BSR, including an optimal temperature of 50–55 °C, an alkaline pH preference (pH 8.5), and tolerance toward various organic solvents and metal ions. Furthermore, the engineered cells hydrolyzed all seven tested vegetable oils, with net FFA release ranging from 3.02 to 5.34 mmol/g wet cells. In repeated palm oil hydrolysis, 38.66% of the initial hydrolytic activity was retained after five cycles. These results demonstrate that the characteristic catalytic properties of BSR can be retained in a recoverable whole-cell format with catalytic functionality toward natural lipid substrates.</div></div><div><h3>Importance</h3><div>Lipases that remain active under alkaline conditions and in the presence of organic solvents are attractive biocatalysts for industrial applications, yet their practical use often requires enzyme purification and immobilization. In this study, we employed yeast surface display to construct a whole-cell catalyst based on the alkaline and solvent-tolerant lipase BSR from <em>Burkholderia gladioli</em>. The engineered yeast largely retained the characteristic catalytic properties of BSR while enabling catalyst recovery and reuse without additional immobilization procedures. Furthermore, the whole-cell catalyst hydrolyzed seven natural vegetable oils and remained catalytically active during repeated palm oil hydrolysis. These findings extend the evaluation of BSR from the purified enzyme to a recoverable whole-cell format and demonstrate its catalytic functionality toward natural lipid substrates.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104287"},"PeriodicalIF":4.6,"publicationDate":"2026-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149200085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intensive vegetable farming in tropical mountains reduces bacterial network complexity and alters key soil taxa","authors":"Gustavo Souza Lima Sant'Anna,&nbsp;Diogo Paes da Costa,&nbsp;Julia Ferreira Xavier,&nbsp;Daniele Rodrigues Barbosa,&nbsp;Maura Santos Reis de Andrade da Silva,&nbsp;Everaldo Zonta,&nbsp;Nelson Moura Brasil do Amaral Sobrinho,&nbsp;Irene da Silva Coelho","doi":"10.1016/j.bcab.2026.104222","DOIUrl":"10.1016/j.bcab.2026.104222","url":null,"abstract":"<div><div>Mountain agroecosystems in tropical regions offer conditions for year-round vegetable cultivation. However, there is limited knowledge about how intensive vegetable cultivation in fragile mountain soils influences the bacterial community. We compared bacterial community and physical and chemical properties of crop and forest soils to assess the impact of agriculture. Crop soils exhibited high pH, phosphorus (P), and potassium (K) values, whereas forest soils had higher exchangeable aluminum (Al³⁺) and potential acidity (LMM, p &lt; 0.05). Alpha diversity was similar between land uses, whereas bacterial community composition differed between crop and forest soils. Based on the multinomial species classification method (CLAM), 14.5% of ASVs were classified as crop specialists, 17.3% as forest specialists, and 6.0% as generalists occurring in both environments. Crop soils showed higher proportions of Bacteroidetes and Proteobacteria specialists, whereas forest soils were characterized by Verrucomicrobia and Acidobacteria specialists. Microbial networks in forests exhibited 138 nodes and 550 links, compared to crop soils with 163 nodes and 350 links. The average degrees were calculated as 7.97 and 4.29 for forest and crop soils, respectively. Despite the observed differences in network complexity, positive correlations dominated in both experimental groups, with prevalences of 64% in forests and 59% in crop soils. High betweenness centrality values were observed in crop soil network, with key species from Actinobacteria and Chloroflexi. In contrast, high-centrality ASVs in forest soils were mainly assigned to Proteobacteria, Verrucomicrobia, and Actinobacteria. Our findings indicate that intensive cultivation alters soil bacterial community structure and reduces bacterial network complexity.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104222"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148938516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated multi-omics analysis of Ghanjivamrit, Jivamrit, and Beejamrit reveals their potential roles in sustainable agriculture","authors":"Smit K. Patel,&nbsp;Aditya P. Rathore,&nbsp;Kartik Gajjar,&nbsp;Rakesh Rawal,&nbsp;Himanshu A. Pandya","doi":"10.1016/j.bcab.2026.104249","DOIUrl":"10.1016/j.bcab.2026.104249","url":null,"abstract":"<div><div>Natural farming inputs such as Ghanjivamrit, Jivamrit, and Beejamrit are traditional cow-based formulations increasingly promoted as sustainable alternatives to chemical-based agricultural inputs. However, despite their increasing adoption and documented agronomic benefits, integrated metabolomic and metagenomic characterization of all three major natural farming inputs within a single experimental framework remains limited, along with efforts to relate their distinct metabolite and microbial profiles to their traditionally reported agricultural applications. The results of present study revealed the presence of diverse and agriculturally important metabolites, including desferrioxamine Et2, 6-hydroxymelatonin, and astilbin in Ghanjivamrit; gallocatechin, citric acid, and questinol in Jivamrit; and rapamycin, tolaasin, and voriconazole in Beejamrit. The majority of identified metabolites belonged to the class of carboxylic acids and derivatives (11.55%), followed by prenol lipids (6.46%) and fatty acyls (4.79%). Each formulation hosted a distinct microbial community composition, with Ghanjivamrit enriched in <em>Acinetobacter</em> (11.94%), Jivamrit in <em>Lactobacillus</em> (17.69%), and Beejamrit in <em>Romboutsia</em> (8.71%), along with dominant fungal genera such as <em>Microidium</em> (30.63%), <em>Trichosporon</em> (63.88%), and <em>Aspergillus</em> (43.98%), respectively. Based on the annotation of metabolite and microbial functions using previously published literature, it is suggested that Ghanjivamrit could promote plant growth and enrich soil carbon and nitrogen content; Jivamrit may enhance plant defense and soil-plant health; while Beejamrit might support seed germination and beneficial plant-microbe interactions. These findings provide scientific evidences for the potential biocatalytic role of natural farming inputs in advancing sustainable agriculture through improved soil and plant health.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104249"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148997024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Groundnut (Arachis hypogaea L.) growth promotion and Aspergillus niger suppression by Lactiplantibacillus plantarum AM and Pseudomonas aeruginosa FCC25","authors":"Vishal L. Handa,&nbsp;Bhoomi N. Patel,&nbsp;Ramesh K. Kothari,&nbsp;B.R.M. Vyas","doi":"10.1016/j.bcab.2026.104251","DOIUrl":"10.1016/j.bcab.2026.104251","url":null,"abstract":"<div><div>Indiscriminate use of chemical fungicides in agriculture can disrupt rhizospheric microbial communities and compromise crop performance, underscoring the need for sustainable alternatives. This study evaluated the effects of <em>Pseudomonas aeruginosa</em> FCC25 and <em>Lactiplantibacillus plantarum</em> AM, alone and with tricalcium phosphate, on groundnut (<em>Arachis hypogaea</em> L.) seed germination and fungal disease incidence under controlled conditions, and plant growth, nodulation, yield, fungal disease incidence and soil properties under field conditions. Their performance was compared with the <em>Fielder</em>^<em>TM</em> (Carbendazim 12% + Mancozeb 63% WP) fungicide. <em>Fielder</em>^<em>TM</em> (Carbendazim 12% + Mancozeb 63% WP) alone delayed seed germination, which remained significantly lower than the control at 48 h (<em>p</em> &lt; 0.0001) but recovered by 96 h. In contrast, <em>Fielder</em>^<em>TM</em> combined with <em>Aspergillus niger</em> promoted early seed germination, outperformed other treatments at 24-48 h. Under controlled conditions, both bacterial inoculants promoted seed germination exceeding 90% at 96 h and reduced fungal infection by &gt;50%. <em>Aspergillus niger</em> treatment suppressed seed germination, and negated bacterial protection when applied in combination with bacterial inoculants. In field trials, bacterial inoculation improved nodulation (96 ± 7 nodules.plant⁻¹), peg formation, biomass, and yield (386 ± 45 g m⁻² for AM). These treatments outperformed <em>Fielder</em>^<em>TM</em>, which suppressed infection but delayed germination and reduced nodulation. Soil analysis revealed that bacterial treatments improved organic carbon, available NPK, and water-holding capacity. Overall, these findings suggest the potential of bacterial inoculants especially with tricalcium phosphate, for improving crop productivity, soil health, and disease suppression. However, further strain-specific biosafety evaluation of <em>P. aeruginosa</em> FCC25 is required before large-scale agricultural application.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104251"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148997036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidative and antihypertensive potential of papain-mediated bull's eye (Priacanthus hamrur) fish skin hydrolysate: Process optimization, structural characterization and cytotoxicity assessment","authors":"Ravi Baraiya,&nbsp;Renuka Vijayakumar,&nbsp;Radhika Rajasree Santha Ravindranath,&nbsp;Joshy Chalil George,&nbsp;Harisankar Kunnamkulathil Chandrababu","doi":"10.1016/j.bcab.2026.104235","DOIUrl":"10.1016/j.bcab.2026.104235","url":null,"abstract":"<div><div>The growing demand for sustainable bioactive compounds has encouraged the valorization of fish-processing by-products into functional ingredients. This study optimized the production of hydrolysate from bull's eye (<em>Priacanthus hamrur</em>) skin and evaluated its antioxidant and antihypertensive activities. Using response surface methodology, optimal conditions were established at enzyme concentration 3%, hydrolysis time 57.06 min, temperature 45°C, and pH 7.50, yielding a hydrolysate with high antioxidant capacity. FTIR spectra displayed characteristic amide I–III bands, confirming peptide bond formation, while XRD patterns indicated an amorphous structure. The fish skin hydrolysate (FSH) showed strong radical-scavenging, reducing, metal-chelating, and lipid-peroxidation-inhibiting activities, which increased proportionally with protein concentration. The hydrolysate also exhibited ACE-inhibitory activity with an IC₅₀ value of 1.60 mg/mL, indicating potential antihypertensive activity. Moreover, the hydrolysate maintained &gt;90% fibroblast cell viability, indicating non-cytotoxicity and biocompatibility. This study demonstrates the novel use of bull's eye skin as a sustainable marine source of multifunctional peptides, supporting the circular bioeconomy and offering promising applications in food, nutraceutical, and pharmaceutical formulations.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104235"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148859080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemically activated biochar for enhancing Zea mays germination: An integrated biochar–ultrasonic seed priming approach","authors":"Joyce dos Santos Farias,&nbsp;Flávia Gomes da Silva,&nbsp;Joanna Elzbieta Kulesza Barros,&nbsp;Milosz Grodzicki,&nbsp;Rafael de Lima Oliveira,&nbsp;Bráulio Silva Barros","doi":"10.1016/j.bcab.2026.104266","DOIUrl":"10.1016/j.bcab.2026.104266","url":null,"abstract":"<div><div>In response to the challenges of climate change and the growing need for sustainable agricultural solutions, this study aimed to develop and evaluate chemically activated soybean seed-derived biochar as a biostimulant for <em>Zea mays</em> seeds, applied through ultrasonic priming, to promote seed germination and early seedling growth. The biochar was produced by pyrolyzing soybean seeds and was chemically activated with KOH, ZnCl₂, or NaBH₄. The synthesized materials were characterized by textural, structural, thermal, spectroscopic, and microscopic techniques. Among the materials tested, the biochar activated with NaBH₄ (BNaBH₄) exhibited superior performance, significantly enhancing germination rate (88%), root length (112.9 mm), and shoot length (71.8 mm), compared to the control. Subsequent trials with different concentrations of BNaBH₄ (0, 1, 25, 50, and 75 mg/L) showed a hormetic pattern: intermediate concentrations promoted early seedling growth. Emergence tests confirmed these findings by showing an increased emergence speed index, a reduced mean emergence time, and greater biomass accumulation at tested concentrations of 1 and 25 mg/L (with regression models predicting optimal responses within the 10–25 mg/L range). Regression analysis revealed a nonlinear, dose-dependent response, reinforcing the observed hormetic behavior and highlighting the need for studies to determine the optimal dose for each biochar type and target crop. These findings highlight that using NaBH₄-activated soybean biochar, combined with ultrasonication, is a promising approach to promoting early vigor in <em>Zea mays</em> seedlings at optimal concentrations.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104266"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149154451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated dark fermentative biohydrogen production from mixed temple waste by cow dung-associated strain and valorization of fermented residues into cellulosic paper","authors":"Poulami Sarkar,&nbsp;Manidipa Roy,&nbsp;Ishita Maji,&nbsp;Soumya Pandit,&nbsp;Piyali Das,&nbsp;M. Kathirselvam,&nbsp;Luhur Akbar Devianto,&nbsp;Srijoni Banerjee","doi":"10.1016/j.bcab.2026.104279","DOIUrl":"10.1016/j.bcab.2026.104279","url":null,"abstract":"<div><div>Underutilized waste biomass from the temple sectors is Lignocellulosic biomass which is generally discarded as waste, polluting the environment and utilization of the potentially valuable resources. This study proposes an integrated circular biorefinery process for biohydrogen production together with valorization of the residue from the bio-fermentation process into biodegradable paper composites. Different pretreatment methods have been applied on the mixed temple waste, to increase the substrate accessibility, and alkaline–hydrothermal pretreatment was found to be the most effective method for dark fermentation. The cow dung-associated strain, <em>Escherichia coli</em> SPAUBT (PQ260983) was used for the production of biohydrogen, which reached a maximum production of 3.48 L H₂/L in 12 h. The Modified Gompertz model was used to describe the fermentation kinetics and gave a highest coefficient of determination of R² = 0.979. After fermentation, the remaining lignocellulosic biomass was harvested and processed for preparation of biomass-reinforced paper composites. The 1% polyvinyl alcohol (PVA) composite formulation showed a favourable swelling capacity, biodegradability and microbiological quality following sterilization, among the formulations tested. In conclusion, the study illustrates a waste-to-energy-and-material approach combining renewable hydrogen production with valorization of post-fermentation biomass, which contributes to better resource recovery and reduction of secondary waste within the circular bioeconomy framework.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104279"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149200086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seed vigor-promoting Enterobacter sp. isolated from the eastern sub-Himalayan terai region serves a dual purpose of potassium solubilization and its retention","authors":"Partha Mahato,&nbsp;Dibyendu Mukhopadhyay,&nbsp;Ashok Chaudhury,&nbsp;Monika Koul,&nbsp;Mohini Singh,&nbsp;Kapudeep Karmakar","doi":"10.1016/j.bcab.2026.104246","DOIUrl":"10.1016/j.bcab.2026.104246","url":null,"abstract":"<div><div>Potassium (K)- solubilizing microbes release K from interlocked silicate clay minerals. These microbes also render it prone to leaching loss as K⁺ is highly water-soluble, especially in the sub-Himalayan Terai region, where rainfall is high. In this regard, K chelation by microbial exopolysaccharide can be an eco-friendly solution. It is known that members of Enterobacterales, which are relatively dominant during the rainy season, can be utilised to devise strategies to prevent K leaching. In this study, two species of soil-dwelling <em>Enterobacter</em> were isolated. Both isolates exhibited K solubilization and exopolysaccharide secretion. Using Salkowski chemistry, their IAA production was estimated (approx. 26 ppm). Furthermore, inoculated soils demonstrated nearly tenfold higher K retention compared to uninoculated controls, highlighting a role of EPS in nutrient binding. These findings reveal that soil-associated Enterobacterales contribute to nutrient availability, soil stability, and plant vigour under the unique conditions of the eastern sub-Himalayan Terai ecosystem.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104246"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148996959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated TiO2 nanoparticles and Pseudomonas sp. GM5 lower tissue chromium concentrations and strengthen physiological resilience in wheat under chromium and drought stress","authors":"Saba Javid Qazi,&nbsp;Humaira Yasmin,&nbsp;Urooj Farid,&nbsp;Zeeshan Ahmad,&nbsp;Asia Nosheen,&nbsp;Tariq Sultan,&nbsp;Naeem Khan,&nbsp;Abdulaziz Abdullah Alsahli","doi":"10.1016/j.bcab.2026.104201","DOIUrl":"10.1016/j.bcab.2026.104201","url":null,"abstract":"<div><div>Chromium contamination and drought can co-occur in agricultural soils and jointly impair wheat establishment, nutrient balance and cellular integrity. This study evaluated titanium dioxide nanoparticles (TiO₂ NPs), the chromium-tolerant plant growth-promoting rhizobacterium <em>Pseudomonas</em> sp. GM5, and their co-application under control, Cr(VI), drought and combined Cr(VI) + drought conditions. Preliminary germination-stage screening identified 500 mg L−1 Cr(VI) as a severe but measurable exposure and 20 mg L−1 TiO₂ NPs as the concentration producing the most favourable seedling response. In the pot experiment, TiO₂ NPs were applied once as a 50 mL soil drench (1 mg pot⁻¹) at stress initiation, whereas GM5 was applied by seed inoculation. Potassium dichromate supplied a nominal 500 mg Cr(VI) kg−1 dry soil; acid digestion followed by atomic absorption spectrophotometry quantified total Cr in tissues and aqua-regia-extractable soil total Cr at harvest. Complete treatment-matched pot growth records and matched shoot and root dry masses were not retained; therefore, biomass responses and organ-specific or whole-plant Cr contents could not be reported, and elemental interpretation was restricted to tissue concentrations and concentration ratios. Relative to untreated combined stress, co-application was associated with 84.2% and 89.2% lower shoot and root total Cr concentrations, respectively, and 57.2–164.4% higher tissue micronutrient concentrations. It also produced more favourable pigment, protein, osmolyte, membrane-injury and antioxidant-enzyme profiles. Root bioconcentration factor declined, whereas translocation factor was interpreted together with absolute tissue concentrations. Overall, co-application produced the most favourable measured concentration and physiological profile under controlled pot conditions. The underlying rhizosphere, transport, nanoparticle–microbe and Cr-speciation mechanisms require direct validation.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104201"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148997035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of drying conditions on biochemical composition and bioactivities of ethanolic extracts from Wedelia chinensis","authors":"Hue Thi Cao,&nbsp;Tan Quang Luc,&nbsp;Duong Dang Huu Hoang,&nbsp;Minh Hoang Ngo,&nbsp;Trang Thuy Thi Nguyen,&nbsp;Quynh Thi Ngoc Le,&nbsp;Tuong Manh Ho,&nbsp;Dung Thi Ha,&nbsp;Muslima Amiriddinova,&nbsp;Aziza Makhmudova,&nbsp;Madinabonu Amiriddinova","doi":"10.1016/j.bcab.2026.104238","DOIUrl":"10.1016/j.bcab.2026.104238","url":null,"abstract":"<div><div>This study investigated the influence of drying temperatures (ambient, 40 °C, 50 °C, and 60 °C) on the biochemical profile and biological activities of <em>Wedelia chinensis</em>. Ethanol extracts (MC-EOS, MC-E40, MC-E50, and MC-E60) were analyzed for their biochemical composition and biological activities. Results showed that drying at 60 °C preserved optimal color and yielded the highest total polyphenol (77.24 ± 1.00 mg GAE/g) and total flavonoid (64.43 ± 2.72 mg QE/g) contents. The MC-E60 extract also exhibited the strongest antioxidant activity, with IC₅₀ values of 79.09, 55.49, and 109.98 μg/mL for phosphomolybdenum, DPPH, and ABTS assays, respectively. In antibacterial evaluation, MC-E60 showed a broad but moderate inhibitory spectrum against both Gram-positive and Gram-negative strains, with MIC values ranging from 512 to 2048 μg/mL. These findings suggest that 60 °C is the optimal drying temperature for maximizing phytochemical recovery and antioxidant activity in <em>W. chinensis</em>. Moreover, the rich phytochemical composition and antioxidant potential indicate the promising application of this species in functional food and nutraceutical formulations.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104238"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148997038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}