Biocatalysis and agricultural biotechnology最新文献

Unraveling latent affinity of strategically designed histidine-rich biosurfactant via tannery waste bio-upcycling in environmentally-relevant lignin removal from pulp and paper industry effluent 通过制革废料的生物循环利用，揭示战略性设计的富组氨酸生物表面活性剂在从制浆造纸工业废水中去除木质素的环境相关性方面的潜在亲和力

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-10-30 DOI: 10.1016/j.bcab.2024.103426

{"title":"Unraveling latent affinity of strategically designed histidine-rich biosurfactant via tannery waste bio-upcycling in environmentally-relevant lignin removal from pulp and paper industry effluent","authors":"","doi":"10.1016/j.bcab.2024.103426","DOIUrl":"10.1016/j.bcab.2024.103426","url":null,"abstract":"<div><div>Bioremediation of pulp and paper industry (PPI) effluent is hindered due to biorefractory lignin. Herein, we demonstrate a lignin-specific designer biosurfactant with synergistic binding sites for enhanced lignin removal from PPI effluent. The histidine rich-cationic lipoprotein biosurfactant (HR-CLB) was synthesized by <em>Bacillus tropicus</em> using tanning industry solid waste, animal fleshing by <em>de novo</em> substrate-dependent synthesis pathway. Interestingly, the HR-CLB anchored functionalized carbon (HR-CLBAFC) demonstrated a high lignin sequestration capacity of 93.2 mg/g HR-CLBAFC at optimized time, 60 min; pH, 5; temperature, 45 °C; and mass of HR-CLBAFC, 1.0 g. The sequestration was confirmed by HR-SEM, UV–Vis., FT-IR, and WCA analyses. The isotherm studies revealed the involvement of Freundlich isotherm (regression coefficient, R<sup>2</sup>: 0.988) in regulating lignin sequestration onto HR-CLBAFC with a Freundlich constant (K<sub>f</sub>) of 16.46 ((mg/g)(L/mg)<sup>1/n</sup>). Moreover, the kinetics studies divulged the contribution of the pseudo-second-order kinetic model (R<sup>2</sup>: 0.992) in regulating the dynamic mechanism of lignin sequestration onto HR-CLBAFC with a rate constant (<em>k</em><sub>2</sub>) of 0.00022 g/mg min. Additionally, the thermodynamics studies discovered a positive Gibbs free energy (ΔG: +170 kJ/mol) and entropy (ΔS°: +130 kJ/mol), indicating the involvement of chemical interaction during the sequestration. Furthermore, the mechanistic study confirmed the role of an incomplete valence shell of nitrogen in histidine centers of HR-CLB in regulating electrostatic interaction with lignin molecules during the sequestration process. Subsequently, the HR-CLBAFC applied for lignin sequestration from the real-time PPI effluent demonstrated an outstanding sequestration efficiency (>99.4%), confirming the unequivocal potential of the HR-CLBAFC matrix in lignin sequestration from real-time PPI effluent.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142573141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The cytotoxic potential of polyphenols extracted from eight lichen species and their antioxidant activity against the cancer cell lines 从八种地衣中提取的多酚的细胞毒性潜力及其对癌细胞株的抗氧化活性

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-10-30 DOI: 10.1016/j.bcab.2024.103424

{"title":"The cytotoxic potential of polyphenols extracted from eight lichen species and their antioxidant activity against the cancer cell lines","authors":"","doi":"10.1016/j.bcab.2024.103424","DOIUrl":"10.1016/j.bcab.2024.103424","url":null,"abstract":"<div><div>This article presents the results of <em>in vitro</em> experiments on the cytotoxic potential of ethanol extracts from 8 lichen species, <em>Cetraria islandica</em>, <em>Cladonia arbuscula</em>, <em>C. digitata</em>, <em>C. gracilis</em>, <em>C. rangiferina</em>, <em>C. uncialis</em>, <em>Platismatia glauca</em> and <em>Pseudevernia furfuracea</em>, against 5 human cancer cell lines - MCF-7, Caco-2, SK-mel-28, U87MG and Jurkat.</div><div>The potential of the re-dissolved and then tested extracts was determined by means of TPC, free radical scavenging activity in ABTS<sup>•+</sup>, superoxide and hydroxyl tests, copper ion reduction activity, and chelating ability of ferrous ion. The highest TPC (114.29 mg GAE/g), ABTS<sup>•+</sup> (440.61 mmol TE/100 g) and hydroxyl (IC<sub>50</sub>: 0.71 mg/mL) radical scavenging activities tests was obtained for the <em>Pseudevernia furfuracea</em> extract, and for the superoxide radical scavenging activity test (IC<sub>50</sub>: 0.98 mg/mL), the highest potential was found for the extracts from <em>P. furfuracea</em> and <em>Cladonia digitata</em>, and for the copper ion reduction activity (88.15 mmol TE/100 g) and chelating ability of ferrous ion (IC<sub>50</sub>: 1.83 mg/mL) tests, the highest potential was shown for the extract from <em>C. digitata</em>. In the cytotoxicity tests, the strongest potential was shown by the <em>P. furfuracea</em> extract against the MCF-7 (IC<sub>50</sub>: 110.84 μg/mL), Caco-2 (IC<sub>50</sub>: 123.86 μg/mL) and U87MG (IC<sub>50</sub>: 107.43 μg/mL) cancer cell lines. A wound scratch test against the MCF-7 using <em>Pseudevernia furfuracea</em> extract showed a 50% reduction in tumor cell proliferation. The qualitative - 28 secondary metabolites from depside and depsidones classes - and quantitative profile of lichen extracts was analyzed by UPLC-Q-TOF-MS/MS.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142593982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilization of water chestnut waste for biohydrogen production and enhanced power generation by stacked microbial fuel cell 利用荸荠废弃物生产生物氢并通过叠层微生物燃料电池提高发电量

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-10-30 DOI: 10.1016/j.bcab.2024.103425

{"title":"Utilization of water chestnut waste for biohydrogen production and enhanced power generation by stacked microbial fuel cell","authors":"","doi":"10.1016/j.bcab.2024.103425","DOIUrl":"10.1016/j.bcab.2024.103425","url":null,"abstract":"<div><div>A novel two-stage approach combining dark fermentation with microbial fuel cell (MFC) technology is proposed which enable biohydrogen production and bioelectricity generation from residual substrate energy. In the present study, batch fermentation of water chestnut waste using <em>Enterobacter aerogenes</em> (MTCC 2822) resulted in the production of biohydrogen. In the batch process, the highest production was 3.2 L/L. Further, single-parameter optimization and multi-parameter optimization were conducted via Response Surface Methodology (RSM) using the Central Composite Design (CCD) model. The maximum biohydrogen reached 3.44 g/L with 55% COD removal. The biohydrogen yield was 7.163 g H<sub>2</sub>/kg COD<sub>reduced</sub> with a maximum production rate of 712 mL/L/h. Further, the waste fermentation medium or spent media was used as a substrate in a Microbial Fuel Cell (MFC) to produce power using <em>Pseudomonas aeruginosa</em> PA1_NCHU as inoculum. MFCs were operated with various concentrations of phosphate buffer in the anolyte. As the output is limited in a single MFC, MFCs were operated in parallel stacks to increase power output. A maximum of 28% increase in the power density was observed in stacked MFCs. The energy recovered from the dark fermentation process was around 10.39% and a single MFC was around 10.67%. Hence, this study highlights the innovative use of agricultural waste and the effective combination of dark fermentation with stacked MFC, presenting a sustainable method of maximizing biohydrogen production and bioelectricity from spent media. By leveraging stacked MFC configuration, the potential of higher power output is demonstrated, underscoring the significance of this integrated system for energy recovery.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced lipase production and characterization from Aeromonas media VBC8: Applications in biodegradation of lubricating oil waste VBC8 培养基气单胞菌产生的强化脂肪酶及其特征：在润滑油废物生物降解中的应用

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-10-29 DOI: 10.1016/j.bcab.2024.103423

{"title":"Enhanced lipase production and characterization from Aeromonas media VBC8: Applications in biodegradation of lubricating oil waste","authors":"","doi":"10.1016/j.bcab.2024.103423","DOIUrl":"10.1016/j.bcab.2024.103423","url":null,"abstract":"<div><div>This study aimed to explore the potential of a novel indigenous strain for the improved production of lipase from castor oil-contaminated soil. Among the various isolates, <em>Aeromonas media</em> VBC8 was found to be the most effective for lipase production. The effect of different inducer oils (olive, peanut, soybean, rice bran, sunflower, coconut, sesame, and fish liver oil) on the biomass of <em>A. media</em> VBC8 and its lipase activity was determined. Among the various oils assessed, fish liver oil exhibited highest lipase activity, with 89 U/mL with 9.1 g/L of biomass. Furthermore, Box-Behnken Design was used to optimize the cultural conditions resulting in an enhanced lipase activity of 1156 U/mL. The lipase was purified through ammonium salt (60 w/v %) precipitation, desalting and ion exchange column, achieving a yield of 16 % and specific activity of 98.4 U/mL. The purified lipase remained active over a wide range of pH 4.0–11.0 and temperature of 10–80 °C with maximum activity at pH 8.0 and 40 °C. SDS-PAGE analysis revealed the lipase's molecular weight to be 94 kDa. The study also evaluated the role of crude and purified lipase in the biodegradability of lubricating oil waste, achieving a maximum fatty acid conversion of 39 and 76 %, respectively, after 7 h incubation at room temperature.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142586187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of shrimp and crab shell as bio-flocculant for Chlorella pyrenoidosa harvesting using response surface methodology 利用响应面方法优化虾壳和蟹壳作为生物絮凝剂用于小球藻收获的效果

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-10-29 DOI: 10.1016/j.bcab.2024.103422

{"title":"Optimization of shrimp and crab shell as bio-flocculant for Chlorella pyrenoidosa harvesting using response surface methodology","authors":"","doi":"10.1016/j.bcab.2024.103422","DOIUrl":"10.1016/j.bcab.2024.103422","url":null,"abstract":"<div><div>The use of bio-flocculants in flocculation processes offers a feasible approach for separating solid and liquid phases, with a particular focus on microalgae harvesting. Critical parameters such as the dose of bio-flocculant, pH level, and dose of cationic inducer significantly impact the success of microalgae harvesting. This study aims to examine the efficacy of chitosan derived from shrimp shells and crab shells as bio-flocculants in microalgae harvesting, specifically <em>Chlorella pyrenoidosa</em>. Central Composite Design (CCD) and Response Surface Methodology (RSM) were employed in this study to attain optimal flocculation conditions, characterized by high flocculation efficiency and short settling time. The optimal harvesting condition using chitosan derived from shrimp shells was achieved at 0.499 g/L of bio-flocculant, pH 9.09, and 0.172 g/L of cationic inducer with 92.58% efficiency and 133.49 s settling time. Meanwhile, The optimal harvesting condition with chitosan derived from crab shells was achieved at 0.434 g/L of bio-flocculant, pH 8.96, and 0.168 g/L of cationic inducer. This resulted in an efficiency of 93.72% and a settling time of 117 s. Based on this fact, the potential of shrimp shells and crab shells as bio-flocculants for <em>Chlorella pyrenoidosa</em> harvesting is evident. Furthermore, the efficacy of flocculation and settling time are significantly influenced by variables such as the concentration of cationic inducers, the levels of bio-flocculants, and pH.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142573140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial synthesis of zinc oxide nanoparticles and their potential biological application as an antimicrobial and anticancer agent 微生物合成氧化锌纳米粒子及其作为抗菌剂和抗癌剂的潜在生物应用

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-10-26 DOI: 10.1016/j.bcab.2024.103417

{"title":"Microbial synthesis of zinc oxide nanoparticles and their potential biological application as an antimicrobial and anticancer agent","authors":"","doi":"10.1016/j.bcab.2024.103417","DOIUrl":"10.1016/j.bcab.2024.103417","url":null,"abstract":"<div><div>Zinc oxide nanoparticles (ZnO-NPs) are a type of metal oxide nanomaterial, recognized as a valuable and adaptable inorganic compound owing to its distinctive physical and chemical properties. Nanosized ZnO particles exhibit substantial antibacterial properties attributable to their diminutive size, which can activate various bactericidal mechanisms within the bacterial cell, including interactions with the bacterial surface or core, the generation of reactive oxygen species (ROS), the release of Zn2+, and potential endocytosis by cells. ZnO NPs nanoparticles were extracellularly produced using pigment extracts from the PP6 strain. Agar well screening indicated that PP6 secondary metabolites possess antibacterial properties. UV–Vis spectroscopy was used to analyze the external growth of nanoparticles. Scanning Electron Microcopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), and X-ray diffraction (XRD) techniques were employed to analyze the shape, stability, crystalline structure, and coating of strain PP6 ZnO NPs. The PP6 ZnO NPs demonstrated an antibiofilm impact on the bacterial pathogens tested, which was dependent on the dosage. Elevated levels of lipid peroxidation (LPO) and reduced antioxidant activity are indicative of apoptosis in cancer cells. The synthesized ZnO NPs nanoparticles were assessed for their anticancer properties by performing the MTT assay on HT-29 human colorectal adenocarcinoma cells. ZnO NPs nanoparticles exposed to HT-29 cells the viability was reduced significantly in proportion to the concentration of nanoparticles. Additional comprehensive study will be needed to fully understand their mechanism.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142578495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the anti-inflammatory effects of carotenoids-rich product from microalgal mass-cultured between natural seawater and magma seawater 比较天然海水和岩浆海水中大量培养的微藻富含的类胡萝卜素的抗炎作用

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-10-24 DOI: 10.1016/j.bcab.2024.103420

{"title":"Comparison of the anti-inflammatory effects of carotenoids-rich product from microalgal mass-cultured between natural seawater and magma seawater","authors":"","doi":"10.1016/j.bcab.2024.103420","DOIUrl":"10.1016/j.bcab.2024.103420","url":null,"abstract":"<div><div>Microalgae can be mass-produced in a short period of time and are rich in high value-added product such as carotenoids, thereby it is widely used in a variety industrial field. However, the production cost expensed a lot due to high price of culture ingredient, vitamins. Additionally, >80% of synthetic carotenoids are used in industrial field, and their cost is also highly expensed. Therefore, in this study, a microalga, <em>Tetraselmis</em> sp. was mass-cultured using natural seawater (NS) and magma seawater (MS) as vitamins. As a result of comparing the general composition between the extracts cultured in two culture media, crude carbohydrate and crude lipid contents were 2.68% and 4.38% higher, respectively, while crude protein content was 4.1% lower in the MS culture media. The extraction yield was 8.6% higher in the MS than the NS culture media. Also, lutein and zeaxanthin were predominantly presented in the species and detected in the microalgal extract cultured in MS was 2- and 8-folds higher, respectively, than in NS. Consequently, the carotenoids-rich extracts cultured with two seawaters were exhibited prominent anti-inflammatory effects by suppressing nitric oxide (NO) overproduction and pro-inflammatory mediator such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX)-2 expression through MAPKSs and NF-κB pathway. Interestingly, extract of microalgal cultured in MS showed a more efficient anti-inflammatory effect. These results suggest that the value of MS in increasing the carotenoids contents with beneficial effects in mass cultivation of microalgae.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142594034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the potential of cyanobacteria as food and investigating its production and nutritional properties 揭示蓝藻作为食物的潜力并研究其生产和营养特性

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-10-24 DOI: 10.1016/j.bcab.2024.103421

{"title":"Unraveling the potential of cyanobacteria as food and investigating its production and nutritional properties","authors":"","doi":"10.1016/j.bcab.2024.103421","DOIUrl":"10.1016/j.bcab.2024.103421","url":null,"abstract":"<div><div>Producing energy and food sustainably is essential in order to be eco-friendly, especially with the increasing global population and the depletion of natural energy sources. Cultivating cyanobacteria has gained worldwide interest because of their efficient use of CO<sub>2</sub> and solar energy. Cyanobacteria have the potential to serve as a viable substitute for food as they do not need fertile land for growth, have minimal nutritional requirements for maximum biomass production, and offer high nutritional value. <em>Arthrospira</em> (<em>Spirulina</em>) and <em>Nostoc</em> are often referred to as “single cell protein” due to their high protein content and are widely marketed as functional food globally. The carbohydrate levels in <em>Nostoc commune</em> and <em>Scytonema bohneri</em> surpass those in <em>Arthrospira</em>. Unlike other algal groups that have monounsaturated and saturated fatty acids, cyanobacteria boast high levels of polyunsaturated fatty acids (PUFA), which are advantageous for health and serve as an intriguing ingredient in cosmetics. <em>Arthrospira</em> is simple to produce on a large scale and has garnered interest in a wide range of uses including food, medicine, aquaculture, nano-technology, dyes, agriculture, cosmetics, and feed. Recent review highlighted the positive nutritional properties of <em>Arthrospira</em>, <em>Nostoc</em> and <em>Aphanizomenon</em>. The biochemical composition of <em>Arthrospira</em> and its potential as a food source were thoroughly discussed. In addition, this study provided a comprehensive study of the single cell protein, elucidating the production process and its limitations and rules and regulations governing the use of single cell proteins. The potential of cyanobacteria as a sustainable food source for future generations was confidently argued.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of Gossypium arboreum 3-ketoacyl-CoA synthase 6 (GaKCS6) gene enhanced leaf epicuticle wax in Gossypium hirsutum L. and improved tolerance against whitefly 过表达 3-Ketoacyl-CoA 合成酶 6 (GaKCS6) 基因可增强 Gossypium hirsutum L. 的叶表皮蜡质并提高其对粉虱的耐受性

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-10-23 DOI: 10.1016/j.bcab.2024.103418

{"title":"Overexpression of Gossypium arboreum 3-ketoacyl-CoA synthase 6 (GaKCS6) gene enhanced leaf epicuticle wax in Gossypium hirsutum L. and improved tolerance against whitefly","authors":"","doi":"10.1016/j.bcab.2024.103418","DOIUrl":"10.1016/j.bcab.2024.103418","url":null,"abstract":"<div><div>Cotton Leaf Curl Virus (CLCuV) is a significant threat to cotton production, as it causes Cotton Leaf Curl Disease (CLCuD). Whitefly serves as a vector for the transmission of this virus. It can be controlled by developing barriers against whitefly infestation. The leaf epicuticle wax acts as a protective barrier against whitefly attacks. Research into wax biosynthesis and the fatty acid elongation pathway has highlighted the role of the 3-ketoacyl-CoA synthase (<em>KCS</em>) gene family in producing very-long-chain fatty acids (VLCFAs) in plants. The 3-ketoacyl-CoA synthase 6 (<em>GaKCS6</em>) gene, isolated from the CLCuV-resistant FDH-170 variety of <em>Gossypium arboreum</em>, was cloned under the control of the <em>Ca</em>MV35S constitutive promoter and transformed into the CLCuV-susceptible <em>Gossypium hirsutum</em> variety CKC-3 resulting in significantly higher leaf epicuticle wax deposition. Overexpression of <em>GaKCS6</em> in the transgenic cotton plants was confirmed through quantitative real-time PCR. The transgenic plants not only exhibited average growth but also showed improvements in agronomic traits. Scanning Electron Microscope (SEM) analysis further validated the enhanced leaf epicuticle wax deposition in transgenic plants compared to non-transgenic (control). A free-choice bioassay against whiteflies demonstrated that the transgenic plants remained free of viral infection, as confirmed by real-time PCR. These findings indicate that increased leaf epicuticle wax deposition in transgenic cotton effectively prevents whitefly attacks and the transmission of CLCuV. It suggests that the <em>GaKCS6</em> gene plays a crucial role in producing leaf epicuticle wax through the VLCFAs biosynthesis pathway.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive review on polyhydroxyalkanoate: Genetic engineering to enhance production and feedstocks assimilation as green alternative for Petrochemical plastics 关于聚羟基烷酸酯的全面综述：通过基因工程提高产量和原料同化，作为石化塑料的绿色替代品

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-10-23 DOI: 10.1016/j.bcab.2024.103419

{"title":"A comprehensive review on polyhydroxyalkanoate: Genetic engineering to enhance production and feedstocks assimilation as green alternative for Petrochemical plastics","authors":"","doi":"10.1016/j.bcab.2024.103419","DOIUrl":"10.1016/j.bcab.2024.103419","url":null,"abstract":"<div><div>Microbial polyhydroxyalkanoates (PHA) are promising biopolymers due to their excellent biocompatibility and biodegradability having the potential to be sustainable plastic alternatives for fossil-derived polymers. Carbon flow and energy metabolism divert towards central carbon metabolism, which limits PHA assimilation. Hence genetic engineering strategies target the strains specifically for enhanced PHA synthesis by up-regulating and knocking down operons, thus regulating the biochemical pathway. This review provides an in-depth understanding of genetics in PHA accumulation and briefly discusses its structural properties. <em>C</em><em>upriavidus</em> <em>necator</em> is the pioneer bacteria for PHA production; others, such as <em>Pseudomonas</em> sp. and <em>Bacillus</em> sp., avail themselves for the robust PHA production capabilities of genetically modified organisms. Genetic engineering techniques used for PHA production have been detailed and discussed like CRISPR based systems have also served as efficient genome editing tools to improve the efficiency of metabolic modification. The most promising methods to boost the yield were highlighted, along with the metabolic paradigms of PHA-producing bacteria and a summary of the range of inexpensive carbon substrates that are used. It also coveres how metabolic modification can support microbial cell factories that use various fermentation techniques and co-production systems to produce PHA using modified strains. Nevertheless, the high cost of production preventing PHA from being commercialised could be by-passed via., genetically modified strains or enriched Mixed Microbial Culture (MMC) as a cheaper option along with the solvent-free downstream processes appear to be a promising bioroute to lower PHA costs.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0