Biocatalysis and agricultural biotechnology最新文献

{"title":"Bioactive potential of date palm by-products (Phoenix dactylifera L.): Optimized extraction and antimicrobial activity against veterinary pathogens","authors":"Nahla Mejri ,&nbsp;Mariem Ben Jemaa ,&nbsp;Hanen Falleh ,&nbsp;Majdi Hammami ,&nbsp;Sylvain Guyot ,&nbsp;Renato B. Pereira ,&nbsp;Hélène Sotin ,&nbsp;Fabrizio Ceciliani ,&nbsp;Latifa Abdennebi-Najar ,&nbsp;Wided Megdiche-Ksouri","doi":"10.1016/j.bcab.2025.103701","DOIUrl":"10.1016/j.bcab.2025.103701","url":null,"abstract":"<div><div>The sustainable utilization of agricultural by-products is essential for environmental and economic benefits. This study uses response surface methodology to extract bioactive compounds from date palm by-products (seeds, perianths, spikelets). Extracts' analyses included total phenolic content, antioxidant activity, antimicrobial activity, and phenolic profiles <em>via</em> UHPLC-UV-MS. Optimal extraction conditions were identified as follows: for seeds, 5 min at 20 °C with 60 % ethanol; for perianths, 4 min at 17 °C with 61 % ethanol; and for spikelets, 4.5 min at 50 °C with 60 % ethanol. Mass spectrometry analysis revealed the presence of 28 secondary metabolites, including phenolic acids, flavonoids, glycosides, and sulfated flavonoids. The extracts showed significant antioxidant activity, with the perianth extract exhibiting the highest DPPH inhibition percentage. The extracts’ antimicrobial activity was tested against nine pathogenic bacteria. The spikelet extract showed the strongest inhibition of <em>E. coli</em>, while the perianth extract effectively inhibited <em>S. marcescens</em>, <em>S. typhimurium</em>, and <em>E. faecalis</em>. Minimum inhibitory concentrations for <em>S. aureus</em> were 0.1 mg/mL for the perianth extract, 0.5 mg/mL for the spikelet extract, and 2.5 mg/mL for the seed extract. Besides optimizing the extraction procedure for phenolic compounds from date palm by-products, the present study demonstrated their antioxidant and antimicrobial properties. These findings underscore the significance of date palm by-products as valuable resources for developing functional products, thereby contributing to sustainability and a circular economy.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103701"},"PeriodicalIF":3.4,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the effect of foliar applied siliceous compounds on reducing seed shattering for the conservation of endangered kalmegh (Andrographis paniculata (Burm. f.) wall. ex nees)","authors":"S. Ranjith Kumar ,&nbsp;A.C. Jnanesha ,&nbsp;S. Bharathkumar ,&nbsp;K. Sravya ,&nbsp;S. Venugopal ,&nbsp;R.K. Lal","doi":"10.1016/j.bcab.2025.103700","DOIUrl":"10.1016/j.bcab.2025.103700","url":null,"abstract":"<div><div>This investigation was conducted to formulate a cost-efficient and dependable technique for the extensive regeneration of the Kalmegh plant, focusing on the challenge of seed shattering during the harvesting process. Across a biennial time frame spanning from 2022 to 2023, diverse siliceous substances were administered through foliar application to address seed-shattering issues and enhance growth parameters. The study was carried out utilizing a Randomized Complete Block Design (RCBD) involving seven treatments and 3 replications. The experimental site is situated in a semi-arid sub-tropical region known for hot summers and comparatively mild winters. Results revealed that the foliar application of potassium silicate at a concentration of 200 mg/L notably boosted plant height (42.86 cm), plant spread (19.68 cm²), root length (14.87 cm), and leaf dimensions (4.51 cm) compared to alternative treatments. Furthermore, the application of potassium silicate at 200 mg/L substantially decreased the number of shattered pods (262), while elevating the count of intact pods (91.23), overall pod tally (524.3), and seeds per plant (4718). Increased silicon levels were detected in capsules, suggesting a heightened silicon deposition in plant tissues after the utilization of siliceous substances. Principal component analysis and correlation assessments were utilized to evaluate the impacts of treatments and the interrelationships among variables. To distinguish treatment variations, PCA was performed on a total of eight parameters and six treatments. Together, PC-1 and PC-2 explained 96.14 % of the variance, with PC-1 accounting for 88.83 % and PC-2 for 7.31 %. Plant height is separated by PC-1; T2 shows negative loading, but the other variables and treatments show positive loading. The number of shattered seeds/pod is the main determinant of PC-2; T7 exhibits negative loading, whilst other seeds show positive loading. The links between shattered and unshattered pods, as well as other variables including plant height, plant spread, leaf size, and root length, regarding the use of siliceous compounds were investigated using correlation analysis. The study highlights the effectiveness of applying siliceous compounds via foliar approaches to augment Kalmegh seed productivity and growth attributes in an environmentally sustainable manner, thereby aiding in meeting the escalating demand for this significant medicinal plant.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103700"},"PeriodicalIF":3.4,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the biological potential of cell-free supernatant of Lysinibacillus odysseyi from buffalo milk","authors":"Susila Mangudi,&nbsp;Srinivasan Pappu","doi":"10.1016/j.bcab.2025.103699","DOIUrl":"10.1016/j.bcab.2025.103699","url":null,"abstract":"<div><div>The cell-free supernatant of bacteria contains naturally produced secondary metabolites that have antibacterial, antioxidant, anti-inflammatory, and anticancer properties, which have significant results for the therapeutic field. The isolated strain was identified as <em>Lysinibacillus odyssey</em>i VSS53 (accession number PP112153) through biochemical and morphological analysis. An antibiotic susceptibility test using 14 antibiotics on the isolated bacterium showed sensitivity to eight, intermediate sensitivity to four, and resistance to two antibiotics. The biosafety assessment through hemolytic and gelatinase assays indicates that the isolated bacterium has been verified as safe. The chemical composition of the cell-free supernatant (CFS) was analyzed using UV–Vis, FT-IR, and GC-MS. The stability and size of the CFS were determined through zeta potential and particle size analysis. The maximum inhibition of antioxidant activity using DPPH (29.94 %), the phosphomolybdate antioxidant assay (68.63 %), the FRAP assay (67.4 %), and the nitric oxide activity (26.23 %). Additionally, the anti-inflammatory activity maximum inhibition is 61.3 %. The antibacterial activity against six pathogenic bacterial strains was examined, with the maximum zone of inhibition observed at 100 mg/mL, and antibiofilm activity results showed the highest percentage inhibition at the 0.1 mg/ml of CFS. The anticancer activity against the MDA-MB-231 cell line, with an IC₅₀ value of 0.00705 mg/ml. Toxicity analysis of the CFS using <em>Artemia franciscana</em> showed healthy growth after 24 and 48 h of observation. In conclusion, the CFS of <em>L. odysseyi</em> has therapeutic uses due to its ability to eliminate microorganisms and cancer cells. Future advancements in medicine could potentially harness these advantageous properties of the CFS of <em>L. odysseyi</em>.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103699"},"PeriodicalIF":3.4,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144654799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant pathology and diseases from a novel bibliometric method","authors":"Shan Chen ,&nbsp;Junsha Wang ,&nbsp;Nanxi Xie ,&nbsp;Kailin Chen ,&nbsp;Yuanzhao Ding","doi":"10.1016/j.bcab.2025.103698","DOIUrl":"10.1016/j.bcab.2025.103698","url":null,"abstract":"<div><div>The study of plant pathology is of vital importance to plant health and agriculture. These diseases impact ecosystems and agriculture and therefore need to be studied in depth. This paper introduces a novel bibliometric analysis method, offering a comprehensive view of the current research landscape, distinct from traditional tools like VOSviewer. The analysis identifies key research directions such as disease resistance, pathogen detection, and treatment strategies while also uncovering future opportunities, especially in integrating big data and machine learning. These technologies promise to revolutionize plant disease management by providing predictive capabilities, personalized treatments, and proactive solutions. By processing vast datasets and continuously learning from new inputs, machine learning models can enhance early detection and intervention, leading to more sustainable and effective management of plant diseases in the future.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103698"},"PeriodicalIF":3.4,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144631967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and biochemical responses of mung bean exposed to different concentrations of nano-CeO2","authors":"Najmeh Kamali-Andani ,&nbsp;Sina Fallah ,&nbsp;Jose R. Peralta-Videa","doi":"10.1016/j.bcab.2025.103690","DOIUrl":"10.1016/j.bcab.2025.103690","url":null,"abstract":"<div><div>Cerium oxide nanoparticles (CeO₂-NPs) are widely used across sectors, including agriculture. However, their overuse has led to environmental dispersion, raising concerns about adverse effects on crops and human health via the food chain. This study investigates the physiological, biochemical, and ultrastructural effects of CeO₂-NPs on mung bean (Vigna radiata L.) under greenhouse and semi-natural conditions. The results reveal both beneficial and harmful impacts, highlighting the need for caution in their application. Foliar spraying of CeO₂-NPs elicited dose-dependent responses. In the greenhouse, 500 mg L⁻¹ significantly increased superoxide dismutase (720 %) and proline accumulation (223 %). Under semi-natural conditions, the same dose enhanced shoot dry weight by 27.1 % compared to the control (p ≤ 0.05). In contrast, exposure to 1000 mg L⁻¹ elevated hydrogen peroxide (50 %) and lipid peroxidation (137 %), reduced chlorophyll (20 %), disrupted cellular ultrastructure, and impaired nitrogen fixation, leading to chlorosis and necrosis. This dose also caused significant reductions in dry weight (18.4 % in greenhouse, 25.4 % in semi-natural) and grain yield (55.9 %) (p ≤ 0.05). Root nodulation was significantly reduced at all tested concentrations (250, 500, 1000 mg L⁻¹), suggesting a toxicity threshold below 250 mg L⁻¹. Seed quality was adversely affected, as shown by decreased iron and zinc levels and increased cerium accumulation (p ≤ 0.05). These findings offer essential insights into the potential risks of CeO₂-NPs in leguminous crops, with implications for food safety and sustainable agriculture.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103690"},"PeriodicalIF":3.4,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144663673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative metagenomic analysis of rice phyllosphere bacterial communities under semi-organic and non-organic farming systems","authors":"Gaby Maulida Nurdin ,&nbsp;Baharuddin Patandjengi ,&nbsp;Tutik Kuswinanti","doi":"10.1016/j.bcab.2025.103695","DOIUrl":"10.1016/j.bcab.2025.103695","url":null,"abstract":"<div><div>In the global cultivation of rice (<em>Oryza sativa</em> L.), synthetic fertilizers and pesticides are used to increase yield and manage pests and diseases. Intensive agriculture disrupts native microbial populations including those in the phyllosphere. This study used metagenomic analysis to compare the diversity and composition of phyllosphere bacterial communities in rice cultivated in semi-organic and non-organic systems. The analysis identified 38 phyla, 76 classes, 166 orders, 343 families, 732 genera, and 1094 bacterial species. Although both systems shared a similar taxonomic composition from the phylum to genus level, the semi-organic samples showed slightly higher microbial diversity and evenness. The most dominant phylum in both cultivation systems was Cyanobacteriota. Bacilliota, Pseudomonadota, and Fusobacteriota were more abundant in the semi-organic system. The dominant species in both systems <em>Thalassoporum komareki, Holzapfeliella floricola, Cyanothece aeruginosa,</em> and <em>Idiomarina baltica,</em> are associated with ecological functions such as photosynthesis, nitrogen fixation, and organic matter decomposition. Semi-organic farming also resulted in a lower relative abundance of putative pathogens, including <em>Xanthomonas oryzae, Burkholderia glumae, Pseudomonas fuscovaginae,</em> and <em>Pantoea ananatis</em>, suggesting that a more balanced microbial community may help suppress pathogens. These findings suggest that semi-organic farming practices have the potential to support a more ecologically stable phyllosphere microbiome, which may, in turn, contribute to improved plant health. Further research is needed to explore the ecological functions and interactions of phyllosphere microorganisms in sustainable rice-cultivation systems.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103695"},"PeriodicalIF":3.4,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144663672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing soil stability: using plant extracts to modify cation exchange capacity in expansive soils","authors":"M.V. Shah ,&nbsp;N.M. Rathod ,&nbsp;D.N. Prajapati ,&nbsp;P.J. Mehta ,&nbsp;R.R. Panchal","doi":"10.1016/j.bcab.2025.103666","DOIUrl":"10.1016/j.bcab.2025.103666","url":null,"abstract":"<div><div>Expansive soils are recognized as problematic in the construction industry due to the presence of specific clay minerals that exhibit significant volume changes upon moisture variation. When these minerals come into contact with water, ionic interactions involving cations and anions activate the swelling behavior of the soil. Among various soil improvement techniques, soil stabilization remains one of the oldest and most widely adopted methods by engineers and geotechnical professionals. This paper introduces an innovative, environmentally sustainable approach that integrates soil and plant chemistry, leveraging the cation exchange capacity (CEC) of soil and the properties of biological plant extracts. CEC refers to the soil's ability to adsorb and exchange positively charged ions (cations). When the availability of free cations is limited, the soil's exchange capacity reduces, which can lead to a form of natural stabilization. CEC can be assessed through various techniques, with the methylene blue spot test being a preferred method due to its simplicity, cost-effectiveness, and rapid execution. Plant-based extracts, rich in anions, can interact with soil cations, effectively neutralizing their effects and contributing to stabilization and strength enhancement. This geobiological stabilization method has demonstrated promising results in terms of cost efficiency and applicability. It offers potential for use in highway embankment slope stabilization, subgrade improvement, forest conservation efforts, and broader ecological preservation initiatives.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103666"},"PeriodicalIF":3.4,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144670994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Green kitchen waste synthesized iron nanoparticles: Antifungal activity and growth promotion in tomato plants” [Biocatal. Agric. Biotechnol. 67 (2025) 1–13 103647]","authors":"Nikita Yadav ,&nbsp;Shalini Jain ,&nbsp;Vartika Parashar ,&nbsp;Priyanka Yadav ,&nbsp;Nidhi Gupta ,&nbsp;Sreemoyee Chatterjee","doi":"10.1016/j.bcab.2025.103696","DOIUrl":"10.1016/j.bcab.2025.103696","url":null,"abstract":"","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103696"},"PeriodicalIF":3.4,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144631968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lysinibacillus fusiformis LF-E2 as a plant growth-promoting bioinoculant for improved rice growth","authors":"Pauldurai Malarvizhi ,&nbsp;Kanagaraj Muthu-Pandian Chanthini ,&nbsp;Ramakrishnan Ramasubramanian ,&nbsp;Arulsoosairaj Deva-Andrews ,&nbsp;Ganesh-Subbaraja Pavithra ,&nbsp;Ponnusamy Murugan ,&nbsp;Yeon Soo Han ,&nbsp;Prabhakaran Vasantha-Srinivasan ,&nbsp;Jarosław Proćków ,&nbsp;Noorah Al Kubaisi ,&nbsp;Sengottayan Senthil-Nathan","doi":"10.1016/j.bcab.2025.103683","DOIUrl":"10.1016/j.bcab.2025.103683","url":null,"abstract":"<div><div>Plant growth-promoting bacteria (PGPB) are crucial for sustainable agriculture, but their effectiveness varies between endophytic and rhizosphere bacteria due to differing ecological and climatic conditions. This study investigated the PGP potential of two bacterial strains, <em>Bacillus subtilis</em> (BS-R) and <em>Lysinibacillus fusiformis</em> (LF-E), as a bioinoculant to promote biocatalytic processes in rice cultivation, isolated from the rhizosphere and endophytic niches of traditional red rice (Sivappu Kavuni), on the widely cultivated rice variety TN1 (<em>Oryza sativa</em> L.) to elucidate their mode of action. In vitro analyses revealed that both strains demonstrated promising PGP traits, including the synthesis of indole-3-acetic acid (IAA), phosphate solubilization, siderophore production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, and hydrogen cyanide (HCN) production. Seed priming with these bacterial strains significantly enhanced seed germination and growth parameters. Among the treatments, <em>Lysinibacillus fusiformis</em> LF-E2 outperformed the control and BS-R strains. LF-E2 promoted early germination (within 23 h), improved seed vigor (1764), increased plant height (47.38 cm), and enhanced root (17.72 cm) and shoot (29.66 cm) lengths. Additionally, LF-E2 increased biomass and yield traits, such as a higher tiller number (64.6), panicles (19), grains per panicle (165), and grain weight (32.602 g per 1000 grains). LF-E2 also elevated levels of total soluble proteins (TSP, 2.334 mg/g FW), total phenolic content (TPC, 27.402 mg/g DW), and chlorophyll (5.6432 mg/g FW) in rice leaves. These findings highlight the potential of LF-E2, as a bioinoculant, improving rice growth and productivity, and offering a sustainable approach to enhancing agricultural outcomes.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103683"},"PeriodicalIF":3.4,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144663671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interference for MIPS1 mRNA results in biofortified lablab (Lablab purpureus L.) lines having reduced phytic acid levels","authors":"Feba Jacob ,&nbsp;Deepu Mathew ,&nbsp;K.B. Soni ,&nbsp;Swapna Alex ,&nbsp;Seeja Thomachan Panjikkaran","doi":"10.1016/j.bcab.2025.103693","DOIUrl":"10.1016/j.bcab.2025.103693","url":null,"abstract":"<div><div>Anti-nutritional factor phytic acid, which causes reduced mineral bioavailability by forming indigestible complexed minerals and charged proteins in livestocks and humans, is a major dietary constraint. Gene <em>Myo</em>-<em>inositol 1-phosphate synthase</em> (<em>MIPS</em>) codes for the enzyme L-<em>myo</em>-inositol 1-phosphate synthase, which isomerizes D-glucose-6-phosphate to D-inositol 3-phosphate, the precursor for phytate (<em>myo</em>-inositol hexa<em>kis</em>phosphate, InsP₆). Lablab pods at the harvestable stage contain a high level of phytic acid. This work describes the development of lablab lines with low phytic acid content through RNA interference of <em>MIPS1</em> gene. Sense- and antisense fragments of the target region in the <em>MIPS1</em> gene were PCR amplified using specific primers and treated with T4 DNA polymerase and dATP. The pRNAi-LIC vector was digested with <em>Sma 1</em>, and treated with T4 DNA polymerase and dTTP. The sense- and anti-sense fragments were inserted to vector, cloned in <em>E. coli</em> and cloning was confirmed through PCR and restriction digestion of plasmids isolated from positive colonies. Through sequencing, the direction of target gene fragments in the vector was confirmed. The recombinant plasmid was transformed into <em>Agrobacterium tumefaciens</em>, and through <em>in planta</em> transformation, six cisgenic lines were obtained. Synthesis of target siRNA was confirmed using stem-loop Reverse Transcription PCR. Comparative expression levels of <em>MIPS1</em> in RNAi lines were downregulated to 0.03–0.75-fold. The phytic acid level in mature pods was reduced significantly from 410.88 mg/100 g in wild type to 56.47–278.53 mg/100 g (32.22–86.25 % reduction). Germination, development and agronomic performance of the RNAi lines were comparable with those of wild type.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103693"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144579412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}