Biocatalysis and agricultural biotechnology最新文献

{"title":"Spent broth of Micromonospora sp. TBJPDA3, a sporocarp bacterium of Calocybe indica, improved mushroom spawning in sawdust substrate","authors":"Toga Pangihotan Napitupulu ,&nbsp;Nadya Jihandariputri ,&nbsp;Atit Kanti ,&nbsp;I Made Sudiana","doi":"10.1016/j.bcab.2025.103673","DOIUrl":"10.1016/j.bcab.2025.103673","url":null,"abstract":"<div><div><em>Calocybe indica</em> is a potential tropical edible mushroom with an attractive taste and a high nutritional value. To obtain a desired harvest, the mycelia should be spawned in the entire sawdust substrate before proceeding to the next step. However, the spawning rate of this mushroom is considerably low. Recently, bacteria isolated from the sporocarp of mushrooms attracted many attentions due to their ability to specifically promote the mycelial growth of their fungal host via the production of metabolites. Therefore, the aim of this study was to accelerate the spawning run of <em>C. indica</em> by using a crude extract of its sporocarp bacterial metabolite. Two isolates with a significant mycelial growth promotion were employed, namely TBJPDA3 and TBJPDA4. The spent broth (SB) of TBJPDA3 and TBJPDA4 significantly stimulated the mycelial growth of <em>C. indica</em> in agar plate by 26.85 and 18.33 %, respectively. In sawdust medium, the SB of TBJPDA3 increased 28.83 % of the mycelial growth. Furthermore, the presence SB of TBJPDA3 in sawdust substrate also increased 26.13, 100.00, and 30.98 % of laccase, lignin peroxidase, and manganese peroxidase activity of the fungus, respectively. The strain of TBJPDA3 was molecularly identified as <em>Micromonospora</em> sp. The untargeted metabolomic toward SB of TBJPDA3 showed collection of some potential carbonecous and nitrogenous compounds. In conclusion, <em>Micromonospora</em> sp. TBJPDA3 and its metabolites were prospective for sustainable <em>C. indica</em> cultivation through accelerating bioconversion of sawdust substrate.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103673"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of magnesium oxide nanoparticles by using Petroselinum crispum extract and their potent antibacterial and wound healing properties","authors":"Özge Çağlar Yıldırım ,&nbsp;Hasan Türkez","doi":"10.1016/j.bcab.2025.103688","DOIUrl":"10.1016/j.bcab.2025.103688","url":null,"abstract":"<div><div>The popularity of plant-mediated green synthesis is increasing due to its simplicity, cost-effectiveness, eco-friendliness and versatility. The synthesis of metal oxide nanoparticles based on plant extracts facilitates the production of non-toxic nanoparticles owing to the presence of several plant chemicals and biochemical compounds which are beneficial for biological and pharmaceutical applications. In the present study, leaf extracts of Petroselinum crispum were used for the synthesis of magnesium oxide (MgO) nanoparticles. The biophysical properties of the synthesized MgO nanoparticles were investigated using ultraviolet–visible spectroscopy. Furthermore, Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) were employed to determine and analyse the functional groups and morphology of the nanoparticles. The maximum peak was found at 210 nm using UV–visible spectroscopy. This finding is indicative of the formation of MgO nanoparticles within the sample. The FTIR results showed that the modifications in the functional groups are responsible for the formation of nanoparticles. The presence of magnesium oxide was confirmed by the peak at 652 cm^-1. Furthermore, the SEM images revealed that the particle size of the samples was in the range of 50–100 nm. The investigation then turned to the assessment of the potential toxicity of the green-synthesized MgO nanoparticles in healthy human dermal fibroblasts. The study revealed that the particles were not toxic in the concentration range of 0–400 μg/mL. Furthermore, the biosynthesised particles have the potential to promote cell proliferation in vitro wound model. In addition, the antimicrobial activity of the MgO nanoparticles against <em>Escherichia coli</em> and <em>Streptococcus aureus</em> bacteria was demonstrated.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103688"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144579414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of different drying techniques on physicochemical and techno-functional properties, gelatinization, pasting behaviors, and morphology of bitter yam (Dioscorea bulbifera L.)","authors":"Monalisa Sahoo ,&nbsp;Sushree Titikshya ,&nbsp;Vivek Kumar ,&nbsp;S.N. Naik","doi":"10.1016/j.bcab.2025.103672","DOIUrl":"10.1016/j.bcab.2025.103672","url":null,"abstract":"<div><div>This study investigates the influence of various drying methods—sun drying (SD), shade drying (SHD), hot air drying (HAD), microwave drying (MWD), infrared drying (IRD), and freeze-drying (FD)—on the physicochemical, nutraceutical, structural changes, and pasting properties of bitter yam flour. Scanning electron microscopy (SEM) analysis revealed that FD samples retained the original starch granule structure, while HAD and MWD samples exhibited substantial damage and variation in particle sizes. FD samples exhibited the highest levels of resistant starch (50.23 %), while IRD and MWD showed lower resistant starch levels (2.05 % and 2.03 %, respectively). Modern drying methods (HAD, MWD, IRD, and FD) achieved a moisture content of less than 10 %, ensuring safer, longer storage, with FD achieving the lowest moisture content (6.07 %). The HAD sample demonstrated the highest peak viscosity (732.17 mPas), suggesting better starch stability compared to MWD (377.17 mPas) and IRD (598.37 mPas). Nutraceutical analysis indicated that FD samples had retained the highest total flavonoid content (75.77 mg/g) and antioxidant activity (FRAP = 21.99 mM Fe (II)/g). These findings suggest that FD, compared to traditional drying methods, preserves the nutritional and functional properties of bitter yam flour.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103672"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144631966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction of phenolic acids, flavonoids, and triterpenes from apple pomace via supercritical Technology: A Systematic review","authors":"Larissa Castro Ampese ,&nbsp;Henrique Di Domenico Ziero ,&nbsp;Jean Augustin Velasquez-Pinas ,&nbsp;Lucia Baldino ,&nbsp;Ernesto Reverchon ,&nbsp;Tânia Forster-Carneiro","doi":"10.1016/j.bcab.2025.103692","DOIUrl":"10.1016/j.bcab.2025.103692","url":null,"abstract":"<div><div>Bioactive compounds can interact with one or more components of living tissue, providing a wide range of potential effects. An alternative source for obtaining bioactive compounds is biomass: it is possible to valorize residual biomass from agricultural activities by reinserting it into the production chain. This work aims to identify trends in the use of bioactive, phenolic, and antioxidant compounds from apple pomace (AP) and to evaluate how supercritical fluid extraction (SFE) can be applied for this purpose. To this end, a bibliometric analysis is carried out to highlight the areas of study that are already well developed and to verify the existing opportunities for new studies. By evaluating the studies retrieved in this bibliometric search, it is possible to observe the evolution of research topics around characterizing, obtaining, and applying bioactive compounds from AP. Some opportunities for study lie in the development of sustainable extraction techniques, providing the recovery of bioactives with less environmental impact, as well as the development of economically viable processes with increased scale in the functional foods and pharmaceutical applications sectors. Finally, the integration of various methods for the exhaustive use of apples and their pomace is pointed out as a trend for future studies, leading to the circularization of the production chain through the implementation of biorefineries.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103692"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144596401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing camelina performance under drought conditions through the combined action of nanochitosan-encapsulated rosemary oil and arbuscular mycorrhizal fungi","authors":"Mohammad Haghaninia ,&nbsp;Abdollah Javanmard ,&nbsp;Farzad Rasouli ,&nbsp;Emanuele Radicetti ,&nbsp;Samaneh Memarzadeh Mashhouri","doi":"10.1016/j.bcab.2025.103682","DOIUrl":"10.1016/j.bcab.2025.103682","url":null,"abstract":"<div><div>Camelina is recognized for its high oil content and richness in α-linolenic acid, a key precursor for omega-3 fatty acid biosynthesis; however, it is increasingly affected by drought stress associated with climate change. This study evaluated the effects of rosemary essential oil encapsulated in nanochitosan (NPs), applied individually or combined with arbuscular mycorrhizal fungi (AMF), on growth, physiological traits, antioxidant defense, and oil yield and composition of camelina under different irrigation regimes. A two-year field experiment was conducted using a split-plot design based on a randomized complete block with three replications. The main plots included full irrigation throughout the growing season (IR1), limited irrigation from flowering (IR2), and limited irrigation from pod formation (IR3). Subplots comprised no treatment, foliar application of NPs, AMF inoculation, and the combined use of NPs and AMF. Drought stress, particularly when initiated from flowering, markedly impaired photosynthetic pigments, nutrient assimilation, relative water content, seed yield, oil-related traits, and the extent of mycorrhizal colonization. In contrast, the combined application of NPs and AMF, compared to no treatment and individual applications, significantly improved seed and oil yield alongside enhanced chlorophyll fluorescence, nutrient availability, accumulation of antioxidant metabolites including phenolics, flavonoids, ascorbic acid, proline, soluble sugars, and tocopherols. It also increased mycorrhizal colonization, stimulated enzymatic antioxidant defenses, and reduced oxidative stress markers such as malondialdehyde and hydrogen peroxide. Additionally, the oil profile improved through an increased proportion of unsaturated fatty acids accompanied by a decrease in saturated fatty acids. Overall, the results suggest that the co-application of nanochitosan-encapsulated rosemary essential oil and AMF may offer a sustainable and potentially effective strategy to enhance drought tolerance and improve seed and oil quality in camelina under water-limited conditions.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103682"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144596523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly degradation of methyl orange using Ralstonia pickettii","authors":"Asranudin ,&nbsp;Adi Setyo Purnomo ,&nbsp;Eirene Grace Fransina ,&nbsp;Holilah ,&nbsp;Agus Wedi Pratama ,&nbsp;Alya awinatul Rohmah ,&nbsp;Silvia Abdi Pratama ,&nbsp;Khanom Simarani ,&nbsp;Setyo Budi Kurniawan ,&nbsp;Ulfa Dwiyanti ,&nbsp;Muhammad Iqbal Hidayat","doi":"10.1016/j.bcab.2025.103658","DOIUrl":"10.1016/j.bcab.2025.103658","url":null,"abstract":"<div><div>The widespread problem of dye pollution, with hazardous chemicals like methyl orange (MO) contaminating water sources, poses a significant threat to both the environment and human health. This study explored a promising and environmentally friendly solution: biodegradation of MO using the bacterium <em>Ralstonia pickettii</em>. The results demonstrated that <em>R. pickettii</em> effectively decolorized MO (100–200 mg/L) by 98 % under static conditions. The optimal conditions for decolorization were found to be a pH of 7 and a temperature of 40 °C. The decolorization of MO was maximized when the growth medium contained substrates like lactose broth, ammonium sulfate, urea, yeast extract, and gelatin. The culture exhibited significant decolorization capacity for 48 h. The activities of enzymes like laccase, NADH-DCIP reductase, and azoreductase suggest their involvement in breaking down MO structure. Metabolites formed after decolorization, including N, N-dimethylaniline (m/z 121), 4-amino-2,6-dihydroxybenzoate (m/z 169), and (2Z,4E)-5-amino-2,5-dihydroxypenta-2,4-dienoate (m/z 144), were characterized using FTIR and LC-TOF/MS. Phytotoxicity test showed the MO was toxic and metabolites obtained after its decolorization were nontoxic for the mungbean plant.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103658"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144517499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic synthesis of Albizia lucidior mediated magnetite nanoparticles (Fe3O4 NPs): Therapeutic applications and photocatalytic activity","authors":"Vrushali Manoj Hadkar ,&nbsp;Nayan Kumar Sishu ,&nbsp;Swathy Prakash Jayabharathi ,&nbsp;Arunagiri Sharmila ,&nbsp;Chinnadurai Immanuel Selvaraj","doi":"10.1016/j.bcab.2025.103676","DOIUrl":"10.1016/j.bcab.2025.103676","url":null,"abstract":"<div><div>This study presents the biogenic synthesis of magnetite (Fe₃O₄) nanoparticles using <em>Albizia lucidior</em> ethanolic leaf extract, a novel green approach leveraging the plant's rich phytochemical profile, including phenols (157.37 ± 1.69 mg GAE/g), flavonoids (224.65 ± 1.74 mg QE/g), and terpenoids (231.08 ± 1.90 mg LNE/g) for the first time. Characterization techniques of nanoparticles, including XRD, FTIR, UV–Vis, AFM, FESEM and EDX were performed. FESEM and XRD confirmed the formation of spherical NPs with a crystallite size of 12.28 nm. The NPs exhibited significant antioxidant activity (DPPH scavenging: 64.55 ± 0.49 %; metal chelation: 73.77 ± 0.6 %) and demonstrated cytotoxic effects against HeLa (45.11 ± 1.4 %) and MCF-7 (53.88 ± 0.6 %) cell lines. Photocatalytic studies revealed 60.32 % degradation of sunset yellow dye by NPs under sunlight. The phytotoxicity assays indicated a growth-stimulating effect on <em>Vigna radiata</em> seeds. These significant findings underscore the potential of <em>A. lucidior</em> mediated Fe₃O₄ NPs as innovative nano-formulation, offering mechanistic insights into therapeutic actions, while also serving as effective tools for environmental protection through effective pollutant degradation.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103676"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144548499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical and nutraceutical characterization of coproducts obtained during the protein concentrate preparation from azufrado common (Phaseolus vulgaris) bean grains","authors":"Laura Gabriela Espinosa-Alonso ,&nbsp;Jocelyn de Jesús Gálvez-Morales ,&nbsp;Chibuike C. Udenigwe ,&nbsp;Alma Leticia Martinez-Ayala ,&nbsp;Maribel Valdez-Morales ,&nbsp;Angel Valdez-Ortiz ,&nbsp;Sergio Medina-Godoy","doi":"10.1016/j.bcab.2025.103681","DOIUrl":"10.1016/j.bcab.2025.103681","url":null,"abstract":"<div><div>Common beans are recognized as an alternative source of protein, and valorizing their coproducts aligns with the 2030 Agenda for Sustainable Development Goals. This study aimed to evaluate the nutritional and phytochemical properties of three coproducts generated during production of Azufrado Higuera common bean protein concentrates: grinding coproduct (GCP), alkaline extraction coproduct (AECP), and isoelectric precipitation coproduct (IPCP).</div><div>The coproducts were obtained from the grinding step, alkaline extraction residue, and supernatant from isoelectric precipitation. Their composition, including starch, protein, dietary fiber, minerals and bioactive compounds (phenolics, flavonoids, tartaric esters, and condensed tannins), as well as antioxidant activity were analyzed. Starch was predominant in AECP (61.61 g/100 g dry matter [DM]), followed by GCP (21.25 g/100 g DM) and IPCP (1.99 g/100 g DM). Crude protein was highest in IPCP (36.07 g/100 g DM), followed by GCP (28.17 g/100 g DM) and AECP (6.12 g/100 g DM). Total dietary fiber was most abundant in GCP (39.22 g/100 g of DM). Mineral analysis shows similar calcium and iron content across all coproducts, with IPCP exhibited magnesium and zinc content. Regarding bioactive compounds, IPCP demonstrated the highest phenolic compounds content and antioxidant capacity, while GCP was richer in flavonoids, flavonols, tartaric steres and condensed tannins. In conclusion, the nutritional and phytochemical properties of these coproducts derived from the bean processing industry highlight their significant potential as ingredients for functional food formulation. Furthermore, their utilization offers a promising pathway for reducing waste, minimizing environmental impact, and enhancing the sustainability of the bean processing industry.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103681"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of culture medium for Lacticaseibacillus paracasei DTA-83 to produce biopreservatives enhancing meat product durability","authors":"André Fioravante Guerra ,&nbsp;Breno Pereira de Paula ,&nbsp;Elton Luis dos Santos Gomes ,&nbsp;Carlos Alberto Guerra ,&nbsp;Lucas Marques Costa ,&nbsp;Maria Alice Zarur Coelho ,&nbsp;Rosa Helena Luchese ,&nbsp;Alessio Giacomini ,&nbsp;Viviana Corich","doi":"10.1016/j.bcab.2025.103684","DOIUrl":"10.1016/j.bcab.2025.103684","url":null,"abstract":"<div><div>The study aimed to produce a biopreservative using <em>Lacticaseibacillus paracasei</em> DTA-83 in an axenic culture system with an optimized medium derived from de Man, Rogosa, and Sharpe (MRS) broth. The effects of peptone, yeast extract, glucose, tribasic ammonium citrate, sodium acetate, magnesium sulfate, and dipotassium phosphate on biomass production and medium acidification by <em>L. paracasei</em> DTA-83 were evaluated using a fractional factorial design, followed by a central composite rotatable design. The biopreservative's ability to improve the durability of vacuum-packed cooked sausages, bacon, and sliced cooked ham, produced on an industrial pilot scale, was assessed using the microbial growth predictor MicroLab_ShelfLife. Biopreservatives produced with original MRS broth and untreated samples served as controls. Results showed glucose, yeast extract, and dipotassium phosphate were crucial for both biomass production and acidification. Peptone and sodium acetate significantly influenced biomass production, while tribasic ammonium citrate mainly affected acidification. Magnesium sulfate had no significant impact on either. The optimized biopreservative significantly enhanced meat durability compared to non-optimized and control groups, with a synergistic effect observed when combined with cold storage. These findings suggest that <em>L. paracasei</em> DTA-83 has distinct nutritional needs for biomass production versus acidification, requiring different media compositions for inoculum preparation and biopreservative production. This approach could also be extended to complex media, such as fermented plant extracts or byproducts, in future research.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103684"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144579413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of glutamic acid production capacity of Stenotrophomonas sp. strain CG2 isolated from soil","authors":"Cihat Guner ,&nbsp;Ertan Ermis ,&nbsp;Kubra Ozkan Guner","doi":"10.1016/j.bcab.2025.103665","DOIUrl":"10.1016/j.bcab.2025.103665","url":null,"abstract":"<div><div>Glutamic acid is a widely used amino acid in the food and pharmaceutical industries due to its role as a flavor enhancer and a metabolic precursor. This study aimed to identify glutamic acid-producing bacterial strains from soil samples collected across different regions. Among 262 isolates screened, <em>Stenotrophomonas</em> sp. strain CG2 exhibited the highest production capacity and was identified through 16S rRNA sequencing. Fermentation parameters including pH, temperature, incubation time, and agitation speed were optimized using the Plackett–Burman design, leading to a maximum yield of 3.76 ± 0.65 g/L under optimized conditions (pH 7.0, 30 °C, 200 rpm, 84 h), compared to 2.72 g/L in unoptimized TSB medium. The produced glutamic acid was purified using ion-exchange resin, yielding a recovery efficiency of 53.48 ± 3.28 %, and its identity was confirmed by FT-IR, RAMAN, and LC-MS/MS analysis.</div><div>This study is among the first to systematically explore Stenotrophomonas spp. for glutamic acid biosynthesis under optimized fermentation conditions. The results provide insight into the strain's specific responses to nutrient composition, revealing its potential for future biotechnological applications. By expanding the microbial landscape of amino acid producers, this work offers a foundation for using CG2 in sustainable bioproduction processes, particularly those leveraging food or agro-industrial waste streams.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103665"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}