Biocatalysis and agricultural biotechnology最新文献

{"title":"Biochemical characterization of a partially purified thermostable alkaline protease of Bacillus cereus isolated from a slaughterhouse effluent","authors":"Kehinde O. Awojobi ,&nbsp;Juliet Oluwayemisi Richard-Omole","doi":"10.1016/j.bcab.2024.103439","DOIUrl":"10.1016/j.bcab.2024.103439","url":null,"abstract":"<div><div>Proteases are applicable in the leather industry but with high production costs. There is a need to produce low-cost proteases with sufficient purity for animal skin dehairing. Hence, this study extracted, partially purified, biochemically characterized and applied alkaline protease synthesized by <em>Bacillus cereus</em> to remove animal hair. A crude alkaline protease optimally synthesized by <em>Bacillus cereus</em> in skimmed milk-submerged fermentation medium was purified by glycerol precipitation and CM-Sephadex C-50. The purified enzyme was classified and the kinetic parameters were determined. The purified protease had 723.1 U/mg specific activity at 1.5 purification fold. Purified protease was stable over widely-ranging pH (3–11) and temperature (20–80 °C), with the highest activity at pH 7.0 and 50 °C. The enzyme was specific for casein, with utmost stability at 60 °C, and maintained most of its activity for 60 min. Na⁺, K⁺, Fe³⁺, and NH₄⁺ inhibited the activity, while Ca²⁺ and Al³⁺ increased the activity. The K_m and V_max were correspondingly 4.82 mg/ml and 46.62 U/ml/min. The purified enzyme has the prospect of removing animal hair in the leather industry.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"62 ","pages":"Article 103439"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Metarhizium anisopliae on the histochemistry and enzymatic activities of Helicoverpa armigera (Hübner, 1809)","authors":"Yamini R ,&nbsp;Shanmugam P.S ,&nbsp;Murugan M ,&nbsp;Geetha N ,&nbsp;Meenakshi P ,&nbsp;Srinivasan T ,&nbsp;Premalatha K ,&nbsp;Kandan A ,&nbsp;Sathiyaraj G ,&nbsp;Vivekanandhan P","doi":"10.1016/j.bcab.2024.103443","DOIUrl":"10.1016/j.bcab.2024.103443","url":null,"abstract":"<div><div><em>Metarhizium anisopliae</em>, is widely utilized as a biocontrol agent in agriculture due to its diverse mechanisms of action and adaptability to various field conditions. However, the immune responses of the target insects significantly influence <em>M. anisopliae</em> virulence against insect pests. Among these pests, the tomato fruit borer, <em>Helicoverpa armigera</em>, stands out as a polyphagous and economically significant species for its resistance to numerous insecticides. Understanding the interaction between <em>M. anisopliae</em> and <em>H. armigera</em> immune defense is therefore critical for developing effective biocontrol strategies. In this study, five isolates of <em>M. anisopliae</em> were evaluated against the second and fourth instars of <em>H. armigera</em> using leaf dip-bioassay under controlled laboratory conditions. The isolate ICAR SBI Ma 08 was the most effective against both instars, with lowest LC₅₀ (2.56 × 10⁶ &amp; 3.56 × 10⁸) and LT₅₀ values (4.36 and 6.36 days). The order of efficacy of other strains was ICAR SBI Ma 01 &gt; ICAR SBI Ma 69 &gt; ICAR SBI Ma 04 &gt; ICAR SBI Ma 172. This isolate effectively evaded the immune response of <em>H. armigera</em> by inhibiting the activities of detoxifying enzymes such as esterase, mixed-function oxidase, acetylcholinesterase, and polyphenol oxidase. Conversely, following infection with this potent strain, larvae exhibited increased activities of glutathione S-transferase, catalase, aryl-acylamidase, and peroxidase. An increase in spore concentration had a positive impact on <em>M. anisopliae</em> pathogenicity. Histopathological studies showed that ICAR SBI Ma 08 successfully breached the first epidermal layer, with mycelia reaching the promeristem within 24 h post-infection. <em>M. anisopliae</em> ICAR SBI Ma 08 exhibited the highest efficacy against the second instar with a reduced LT₅₀, and its modulating enzymatic activity suggested that this isolate could be effectively deployed against the first and second instars of <em>H. armigera</em>. Developing an appropriate formulation and ascertaining its efficacy at the field level will confirm ICAR SBI Ma08's potential as a mycoinsecticide.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"62 ","pages":"Article 103443"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gac fruit (Momordica cochinchinensis Spreng): From nutritional value to food processing technology","authors":"Siti Alyani Mat ,&nbsp;Hesham Ali El Enshasy ,&nbsp;Nadirah Abd Rahim ,&nbsp;Xin Nih Chong ,&nbsp;Sajetra Devi Vadiveloo ,&nbsp;Harisun Ya'akob ,&nbsp;Daniel Joe Dailin ,&nbsp;Dennis Sing Teong Chew ,&nbsp;Nor Hasmaliana Abdul Manas","doi":"10.1016/j.bcab.2024.103444","DOIUrl":"10.1016/j.bcab.2024.103444","url":null,"abstract":"<div><div>Gac (<em>Momordica cochinchinensis</em> Spreng.) fruit, which originates from Southeast Asia, is widely recognized for its rich nutritional profile and its traditional medicinal applications. The fruit's commercialization in functional food products is driven by its potent antioxidant, antibacterial, anti-inflammatory, anticancer, neurotropic, and immunomodulatory properties. Additionally, Gac fruit is not widely consumed commercially as food, making it less competitive in the food market. However, processing can compromise these benefits due to the degradation of active compounds under harsh conditions. This review explores the nutrient composition and functional properties of Gac fruit, evaluates common processing techniques, and highlights strategies for nutrient preservation during processing. Emphasizing the importance of continued research, innovative processing, and sustainable practices, the article underscores the potential of Gac fruit to significantly impact global health and wellness. Effective preservation strategies are crucial not only for enhancing consumer health but also for bolstering the economic viability of Gac fruit in the global market.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"62 ","pages":"Article 103444"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizations of ash derived from the crops’ waste biomass for soil improvement and assisted phytoremediation","authors":"Abdulmannan Rouhani ,&nbsp;Valentina Pidlisnyuk ,&nbsp;Karim Suhail Al Souki","doi":"10.1016/j.bcab.2024.103456","DOIUrl":"10.1016/j.bcab.2024.103456","url":null,"abstract":"<div><div>The biomass ash can play a crucial role in agriculture by acting as a valuable fertilizer that fosters nutrient cycles and contributes to the conservation of nutrient's resources. The paper begins by discussion of the chemical properties of ashes derived from the herbaceous and agricultural crops. It then evaluates the effect of biomass ash on the soil properties and plant growth in the field and greenhouse conditions. Finally, the paper evaluates the impact of ash to phytoremediation practices, when nutrients presented in biomass ash were returned to the natural cycles to secure sustainable biomass utilization. Beside nutrients, some potentially toxic elements were presented in biomass ash which can threaten the environment. Rice husk ash followed by bagasse and wheat straw ashes were the most studied materials which showed positive effects on agricultural soil and crops. Furthermore, a positive impact of rice husk ash on the phytoremediation efficiency of <em>Ricinus communis</em> and <em>Vetiver Grass</em> were reported. The results of the current studies show a great potential of biomass ash when applying for the agricultural and environmental remediation actions thorough the careful assessment which ensures the circularity in the revitalization.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"62 ","pages":"Article 103456"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapeseed meal biorefining: Fractionation, valorization and integration approaches","authors":"Phavit Wongsirichot ,&nbsp;Maria Gonzalez-Miquel ,&nbsp;James Winterburn","doi":"10.1016/j.bcab.2024.103460","DOIUrl":"10.1016/j.bcab.2024.103460","url":null,"abstract":"<div><div>Rapeseed meal (RSM) is an agricultural by-product with high potential as a biorefining feedstock due to its abundance, costs, and valorizable fractions, including proteins (such as cruciferin and napin), phenolics (such as sinapine and its derivatives, tannins and lignin), polysaccharides (cellulose, hemicellulose, and pectin), phytic acids and glucosinolates. RSM is also an important candidate for a whole-crop biorefining scheme, due to its integration potential with biorefining of other rapeseed-derived fractions, including rapeseed oil and straw. To date, research and development into holistic integrated RSM biorefining is limited, with the majority of the literature focusing on the valorization of a single fraction, typically proteins or phenolics, overlooking the potential of other fractions as well as the post-processing residue. This review is designed to aid efforts to develop a holistic RSM biorefining scheme through a comprehensive assessment of processing and applications of valorizable RSM fractions within an integrated biorefining context. The myriad of extraction, purification, modification, and applications for each fraction are discussed to elucidate their valorization potential and highlight major gaps within the literature that need to be addressed in the future. Finally, the current literature on RSM biorefinery and a proposal for a more holistic integrated biorefinery scheme are discussed and important considerations, challenges, and future steps for the development of RSM and rapeseed biorefinery are provided. The perspective, insights, and recommendations provided by this review will be beneficial for the future development of holistically integrated biorefineries for rapeseed and other biomass feedstocks in general.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"62 ","pages":"Article 103460"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Economic parameter estimates of spring wheat varieties grown under different cultivation technologies","authors":"Francess Sia Saquee ,&nbsp;Elena Pakina ,&nbsp;Meisam Zargar ,&nbsp;Prince Emmanuel Norman ,&nbsp;Kalabashkina Elena Vladimirovna ,&nbsp;Tsymbalova Vitaliya Alexandrovna","doi":"10.1016/j.bcab.2024.103454","DOIUrl":"10.1016/j.bcab.2024.103454","url":null,"abstract":"<div><div>The demand for wheat is projected to increase by 60% by 2050, highlighting its critical importance. This research aimed to investigate the impact of cultivation technologies on grain yield and the economic efficiency of different cultivation technologies for spring wheat in the non-chernozem zone of the Russian Federation, where most research has focused only on yield and quality comparisons. Field experiments were conducted over two cropping seasons 2022 and 2023, using a split block design with three replications to assess three different cultivation technologies (Basic, Intensive and High-Intensive) and three genotypes (Agros, Belyana and Radmira). Findings revealed that variety and cultivation technology significantly (p ≤ 0.05) affected spring wheat grain yields, agronomic efficiency of potassium (AE_K), and phosphorus (AE_P). Plots amended with basic and high-intensive cultivation technologies exhibited the lowest and highest wheat grain yields of 3.9 and 4.9 t ha⁻¹, respectively. Belyana had the highest grain yield (5.0 t ha⁻¹) followed by Agros (4.9 t ha⁻¹). There was a significant increase in all economic parameter estimates with improved cultivation technologies. Agros exhibited the highest gross revenue over control (42718.0 Rub. ha⁻¹), grain yield value (67900.0 Rub. ha⁻¹), and net return (17537.0 Rub. ha⁻¹) under high intensive cultivation technology, while Radmira had the highest marginal return (0.770) under intensive and variable cost ratio (1.77) under high intensive cultivation technology, whereas Belyana had the lowest values. Findings established that the application of improved cultivation technology maximizes crop yields and net returns, to meet the demands of the growing population.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"62 ","pages":"Article 103454"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive perspective of sustainable nanostructured metal and metal oxide towards agriculture utility for precision farming","authors":"Pooja Singh ,&nbsp;Vanya Nayak ,&nbsp;Ranjana Verma ,&nbsp;Arunadevi Natarajan ,&nbsp;Jay Singh ,&nbsp;Shyam S. Pandey ,&nbsp;Kshitij RB Singh","doi":"10.1016/j.bcab.2024.103457","DOIUrl":"10.1016/j.bcab.2024.103457","url":null,"abstract":"<div><div>The growing advancements in science and technology have brought forward a new domain with the potential to manipulate structures at nano dimensions, termed nanotechnology. Nanotechnology uses materials whose dimensions lie between 1 and 100 nm, which offers them excellent properties and a high surface-to-volume ratio that further enriches it with enormous attention in various domains. Apart from the biomedical and environmental domains, agriculture has also highly utilized nanotechnology in past decades as it carries the global burden of fulfilling food demands and boosting a country's economy. Therefore, developing and enhancing agriculture and its products have become the primary needs of researchers and the government. Moreover, metal-based nanoparticles are highly preferred in the agricultural sector because they can be easily synthesized, readily taken by plants from soil and water, act as excellent antimicrobials, pesticides, and fertilizers, and are also highly used in genetic engineering. Moreover, to reduce their toxicity, biogenic metal-based nanoparticles are synthesized utilizing extracts of plant parts, microorganisms, biomolecules, nutrients, etc. In this article, a systematic review was deliberated by providing pathways to the budding researchers, starting from various types of synthesis of biogenic nanoparticles, factors influencing the formation, and their application in various agricultural sectors. The integration of nanotechnology in agriculture, specifically through the use of biogenic metal-based nanoparticles, addresses key Sustainable Development Goals (SDGs) such as Goal 2: Zero Hunger by enhancing food production and security, Goal 12: Responsible Consumption and Production by optimizing resource use, and Goal 15: Life on Land by promoting sustainable farming practices that minimize environmental impact. Hence, nanoparticle-assisted delivery to plants will lead to precision farming and articulate several challenges in traditional agriculture.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"62 ","pages":"Article 103457"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143178980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial allantoin mediated optimally biosynthesized silver nanoparticles exhibit antioxidant, antibacterial, and seed germination promoting activities","authors":"S.O. Pustake ,&nbsp;S.C. Patil ,&nbsp;P.N. Kirdat ,&nbsp;A.S. Kulkarni ,&nbsp;P.S. Patil ,&nbsp;P.B. Dandge ,&nbsp;K.D. Pawar ,&nbsp;K.D. Sonawane","doi":"10.1016/j.bcab.2024.103448","DOIUrl":"10.1016/j.bcab.2024.103448","url":null,"abstract":"<div><div>The biosynthesis of silver nanoparticles (AgNPs) presents a sustainable, cost-effective, and scalable alternative to conventional synthesis techniques. This study investigates microbial allantoin as a biogenic reducing agent for the synthesis of AgNPs, emphasizing their antioxidant, antibacterial, and seed germination enhancement properties. Optimally synthesized allantoin-mediated AgNPs exhibited a crystalline size of 3.66 nm with a band gap energy of 3.39 eV. Scanning Electron Microscopy (SEM) analysis showed spherical nanoparticles with an average particle size of 84 nm, while particle size analysis revealed a zeta potential of −26.7 mV and a hydrodynamic diameter of 137.1 nm. The AgNPs displayed significant free radical scavenging activity, achieving 58% and 70% inhibition in H₂O₂ and DPPH assays, respectively. Furthermore, these AgNPs demonstrated substantial antibacterial activity against both Gram-positive <em>Staphylococcus aureus</em> and Gram-negative <em>Pseudomonas aeruginosa</em>. Additionally, allantoin-mediated AgNPs promoted germination in <em>Cicer arietinum</em> seeds within 48 h, indicating their potential as plant growth stimulants. This pioneering study on microbial allantoin-derived AgNPs suggests promising applications across pharmaceuticals, environmental science, and cosmetic fields.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"62 ","pages":"Article 103448"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study on the production and immobilization of stem bromelain using polyacrylamide gel matrix and arginine coupled magnetic nanoparticles for enhanced operational stability","authors":"Fariha Javaid ,&nbsp;Madeeha Shahzad Lodhi ,&nbsp;Muhammad Tahir Khan ,&nbsp;Muhammad Sarwar ,&nbsp;Zahoor Qadir Samra","doi":"10.1016/j.bcab.2024.103451","DOIUrl":"10.1016/j.bcab.2024.103451","url":null,"abstract":"<div><div>Protein engineering approaches such as immobilization can be employed to enhance thermal and long-term stability of enzyme. Immobilized bromelain (Brm) has emerged as a critical biocatalyst for optimization in food, textile, cosmetic and pharmaceutical industries. In our study, polyacrylamide, agarose gel matrix and arginine coupled magnetic nanoparticles were synthesized to use as supports for immobilization of stem bromelain. The objective of our study was to compare operational stability of bromelain using various support systems. Our findings showed that the Brm/arg/MNPs had a strong affinity for the substrate, better thermal and pH stability when compared with BrmAG (agarose entrapped Brm) and BrmPG (polyacrylamide entrapped Brm). The rate of reaction was higher for Brm/arg/MNPs (Vmax 23.8 U/ml) than soluble enzyme (7.4 U/ml), BrmAG (Vmax 16.4 U/ml) and BrmPG (Vmax 12.9 U/ml). During storage at 4 °C, BrmAG showed 72% activity while BrmPG retained only 32% activity after 30 days when compared to Brm/arg/MNPs's activity which remained nearly unaffected up to 40 days and it retained its 80% activity for 90 days. For operational stability, Brm/arg/MNPs exhibited 80% of its activity after 15 cycles and only 2% activity loss was observed after 21 cycles. BrmAG and BrmPG retained only 15% and &gt;2% activity after 15 cycles respectively. Our findings showed that arginine coupled magnetic nanoparticles have effectively enhanced the operational and storage stability of bromelain compared to agarose and polyacrylamide gel matrices.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"62 ","pages":"Article 103451"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic analysis and overexpressing the acetyl-CoA carboxylase of oleaginous Saccharomyces cerevisiae CU-TPD4 for enhanced lipid production from agricultural residues","authors":"Thanaphong Tangwanaprai ,&nbsp;Surisa Suwannarangsee ,&nbsp;Kan Tulsook ,&nbsp;Sa-ngapong Plupjeen ,&nbsp;Chompunuch Glinwong ,&nbsp;Warawut Chulalaksananukul","doi":"10.1016/j.bcab.2024.103450","DOIUrl":"10.1016/j.bcab.2024.103450","url":null,"abstract":"<div><div>Single-cell oil produced using oleaginous yeasts is emerging as a promising source for biofuels and oleochemicals. <em>Saccharomyces cerevisiae</em> is the model yeast and is widely utilized in several industrial applications. This study presents a genome analysis of the oleaginous <em>S. cerevisiae</em> strain CU-TPD4, which has been reported to accumulate a high amount of lipids. The analysis revealed a genome size of 17.5 million bases and 7619 predicted genes were identified. To understand the differences between oleaginous and non-oleaginous <em>S. cerevisiae</em>, a comparative analysis was conducted. The results identified 5169 shared gene clusters, 36 unique clusters in <em>S. cerevisiae</em> S288C and 22 unique clusters in <em>S. cerevisiae</em> CU-TPD4. Notably, mutations in proteins associated in lipid biosynthesis that affect phosphorylation, such as ACC1, ALE1, and FAA4, were observed, influencing lipid accumulation. Additionally, overexpression of the <em>ACC1</em> gene under different promoters revealed an approximately 16% improved lipid production under the <em>ENO2</em> promoter compared to the wild-type strain. Subsequently, lipid production process using rice straw hydrolysate was evaluated. The strain produced a highest total lipid of 1.60 g/L, corresponding to a lipid content of 34.12%. Interestingly, the FA composition of the lipids obtained from the rice straw hydrolysate was comprised of more than 75% monounsaturated FAs. This study provides the first draft genome of oleaginous <em>S. cerevisiae</em> CU-TPD4 and insights into genetic improvements to facilitate lipid production.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"62 ","pages":"Article 103450"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}