Biocatalysis and agricultural biotechnology最新文献_第10页

Lytic polysaccharide monooxygenases producing microbes: A key indicator for biomass-degrading enzymes 产生溶解性多糖单氧酶的微生物：生物质降解酶的关键指标

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-08-05 DOI: 10.1016/j.bcab.2024.103337

引用次数: 0

Impact of co-application of iron and silicon on the root plaque formation in rice plants (Oryza sativa L.) grown in arsenic infested soil of gangetic Bengal, India 在印度孟加拉湾砷污染土壤中种植的水稻（Oryza sativa L.）共同施用铁和硅对根斑形成的影响

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-08-05 DOI: 10.1016/j.bcab.2024.103335

{"title":"Impact of co-application of iron and silicon on the root plaque formation in rice plants (Oryza sativa L.) grown in arsenic infested soil of gangetic Bengal, India","authors":"","doi":"10.1016/j.bcab.2024.103335","DOIUrl":"10.1016/j.bcab.2024.103335","url":null,"abstract":"<div><p>The present work is a biomarker-based analysis to understand reduction in arsenic toxicity in rice plants due to co-application of iron and silicon. Both iron and silicon have been co-applied in the soil of the potted rice plants with a specific dose of arsenic. The results showed, increase in root arsenic content with only Fe application (E1) as 115% and decrease in shoot arsenic content as 35.4% against control, with Fe + Si application (E2) the root arsenic increased 44% compared to E1 and shoot arsenic content decreased almost 50%, 73% and 25% increase in root Fe content in E1 and E2 is due to formation of Fe-plaque on the root surface which is further justified by the SEM image. The increased arsenic content in the plant root is due to deposition of arsenic in the root Fe-plaque. Plant health parameters revealed that 18% increase in Carbohydrate in E1 compared to control and 9% increase in E2 compared to E1, chlorophyll and protein content followed a similar trend. Malonaldehyde (MDA) content revealed a decrease of 20% in E1 and E2 plants. Super Oxide-dismutase (SOD), an antioxidant enzyme, the unique biomarker to understand the plant stress, showed a decreasing trend of 11% in E1 compared to control and more than 50% in E2 compared to E1, the expression study of the SOD-gene also showed a positive correlation with the biochemical data.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141954059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insilico sequence-structure based analysis of bacterial chromate reductase to unravel enzymatic specificity towards chromium pollution 基于序列结构的细菌铬酸盐还原酶内部分析，揭示酶对铬污染的特异性

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-08-05 DOI: 10.1016/j.bcab.2024.103339

{"title":"Insilico sequence-structure based analysis of bacterial chromate reductase to unravel enzymatic specificity towards chromium pollution","authors":"","doi":"10.1016/j.bcab.2024.103339","DOIUrl":"10.1016/j.bcab.2024.103339","url":null,"abstract":"<div><p>Extensive use of chromium in industrial sectors leads to hazardous consequences of chromium pollution. The hexavalent chromium is much more damaging to biological systems than the trivalent and other forms. The best strategy for bioconversion of such toxic hexavalent chromium to less toxic trivalent chromium is the enzymatic breakdown using chromate reductase produced by several bacteria. The employment of in silico analysis aids in the quick detection of a protein's function and stability. Therefore, an insilico sequence and structure analysis of chromate reductase of the different bacterial groups has been performed. Amino acid variation reveals more charged polar residues in the extremophilic group than the mesophiles and is more hydrophilic. According to secondary structure data, beta-bulge formation was only observed in <em>Thermus scotoductus</em>. A higher abundance of network salt bridges and isolated aromatic-sulphur interactions were observed in extremophiles, indicating higher stability. Rhombus network salt bridge formation is a novel finding in thermophilic bacteria. Better isolated cation-pi interactions were observed in both thermophiles and halophiles. Molecular dynamics simulation studies through RMSD observed similar trajectories between thermophilic and psychrophilic proteins indicating a shared structural pattern. RMSF studies indicate the lowest fluctuations by thermophiles compared to other groups. Rg values indicate that chromate reductase of <em>Halomonas subglaciescola</em> had the lowest plot indicating its most compactness. The chromate reductase from psychrophilic <em>Psychrobacillus psychrotolerans</em> displayed the lowest SASA, indicating the most stability. Hydrogen bond number was also higher in extremophiles. This is the first report on such comparative analysis of chromate reductase from extremophiles indicating that they are more preferred candidates in providing a more stable enzyme for better chromium remediation.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141963127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable cultivation of Porphyridium cruentum via agro-industrial by-products: A study on biomass and lipid enhancement 利用农用工业副产品可持续栽培蕨类植物：生物量和脂质增加研究

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-08-05 DOI: 10.1016/j.bcab.2024.103341

{"title":"Sustainable cultivation of Porphyridium cruentum via agro-industrial by-products: A study on biomass and lipid enhancement","authors":"","doi":"10.1016/j.bcab.2024.103341","DOIUrl":"10.1016/j.bcab.2024.103341","url":null,"abstract":"<div><p>This study explored the cultivation of <em>Porphyridium cruentum</em> using beet molasses and corn steep liquor (CSL) as alternative agri-waste substrates. The objective was to enhance the biomass and lipid production of <em>P. cruentum</em>, known for its potential as an industrial EPA producer. Using a Box-Behnken Design (BBD) as part of the response surface methodology (RSM), we investigated the effects of beet molasses, CSL, and F/2 nutrients on the microalga's cultivation. The results demonstrated a significant influence of these factors on the final cell count and lipid content over an 8-day cultivation period. Specifically, optimal growth conditions were identified at approximately 1.78 g/L of molasses and 1.89 g/L of CSL, yielding a cell count of 12.1 × 10<sup>6</sup> cells/mL and a lipid content of 24.48%. Validation experiments reaffirmed these findings, with observed results closely aligning with predicted values. Optimized media achieved also a significant higher content of carotenoids and phycobiliproteins compared to the standard media. This research highlights the potential of using agro-industrial by-products for large-scale cultivation of <em>P. cruentum</em>, offering a sustainable approach for enhancing lipid and pigment production.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1878818124003256/pdfft?md5=f008442489d72caa9e18f7caad936c78&pid=1-s2.0-S1878818124003256-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141993409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resilient mechanism of strigolactone (GR24) in regulating morphological and biochemical status of maize under salt stress 绞股蓝内酯（GR24）调节盐胁迫下玉米形态和生化状态的复原机制

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-08-05 DOI: 10.1016/j.bcab.2024.103340

{"title":"Resilient mechanism of strigolactone (GR24) in regulating morphological and biochemical status of maize under salt stress","authors":"","doi":"10.1016/j.bcab.2024.103340","DOIUrl":"10.1016/j.bcab.2024.103340","url":null,"abstract":"<div><p>Strigolactones (SLs), relatively recent plant growth regulators, bolster plant resilience in challenging environmental conditions and augment signaling networks. Salinity, a well-established abiotic stressor, threatens global crop cultivation and productivity. We hypothesized that the synthetic SL analogue GR24 could alleviate salinity stress in maize by reducing oxidative damage and enhancing various growth and physiological attributes. To explore this hypothesis, we subjected two maize hybrids, PB-ProA-2018 and PB-ProA-2019, to seed priming with three GR24 concentrations (non-soaked, water-soaked, 0.001, 0.01, 0.1 mg/L) for 16 h. Seeds were then germinated in sand culture under two salt stress levels [ 0 (Control) and 100 mM NaCl] and nourished with Hoagland's nutrient medium. Salt stress significantly stunted maize growth attributes. The pre-soaking seed treatment with 0.01 mg L⁻<sup>1</sup> GR24 demonstrated a significant enhancement in plant growth metrics, yielding a 31.3% increase in root length and a 62% augmentation in total leaf area per plant, in comparison to non-soaked yet stressed plants. Salt stress led to increased activities of catalase and peroxidase, along with higher levels of leaf ascorbic acid, malondialdehyde, H₂O₂, glycine betaine, and free proline, compared to control seedlings. Pre-soaking with 0.01 mg L<sup>−1</sup> GR24 further upregulated superoxide dismutase (SOD) by 29.6%, peroxidase (POD) by 68.3%, catalase (CAT) by 25%, ascorbic acid by 23.1%, free proline by 42.3%, and total phenolics content by 13%, compared to stressed counterparts. Notably, the 0.01 mg L<sup>−1</sup> GR24 concentration exhibited superior efficacy in mitigating salt stress effects, with PB-ProA-2019 outperforming PB-ProA-2018 among the maize hybrids. These findings advance our understanding of SLs' potential in alleviating salinity stress in maize, offering valuable insights for crop resilience enhancement.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141963128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth characteristics and molecular identification of indigenous Limnospira strains from Ethiopian soda lakes as a protein source 作为蛋白质来源的埃塞俄比亚苏打湖中本地褐藻菌株的生长特性和分子鉴定

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-08-05 DOI: 10.1016/j.bcab.2024.103336

{"title":"Growth characteristics and molecular identification of indigenous Limnospira strains from Ethiopian soda lakes as a protein source","authors":"","doi":"10.1016/j.bcab.2024.103336","DOIUrl":"10.1016/j.bcab.2024.103336","url":null,"abstract":"<div><p>Malnutrition poses a significant global challenge in regions such as Ethiopia, particularly in children with limited dietary diversity. <em>Arthrospira</em>, a protein-rich cyanobacterium known for its rapid growth even under extreme conditions, is a promising solution for biomass production. This study addresses knowledge gaps in the morphological characterization and taxonomic classification of <em>Arthrospira</em> by focusing on precise species identification and strategic strain selection for optimal biomass production. Cyanobacterial strains were cultivated in a modified Spirulina Ogawa Terui medium at 35 °C under the photosynthetically active radiation of 160 μmol photons m<sup>−2</sup> s<sup>−1</sup> with a 12/12 h light/dark period in laboratory conditions. One hundred <em>Arthrospira-</em>like strains were isolated and characterized based on their morphological attributes according to accepted criteria, from Ethiopian soda lakes. Systematic evaluation of the growth rate comparison test identified four exceptionally fast-growing strains, with LC-30 exhibiting the highest dry weight (3.27 ± 0.51 g L<sup>˗1</sup>) and specific growth rate (1.63 ± 0.05 d<sup>˗1</sup>) among them. DNA analysis confirmed that all four examined strains belong to the <em>Limnospira</em> genus, specifically identified as <em>Limnospira fusiformis</em>. LC-30 and LA-08 demonstrated protein and phycocyanin levels (582.80 ± 0.07 and 557.90 ± 0.09 mg g<sup>˗1</sup>, respectively) comparable to those reported for <em>Arthrospira platensis</em>, and surpassing conventional protein sources, thus suggesting their potential for nutritional supplementation. The study highlights the importance of bioprospecting indigenous strains from soda lakes, revealing promising candidates for large-scale biomass production.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141963126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced production of high-value compounds from Chlorella sorokiniana by two-stage cultivation under red light and salinity stress 在红光和盐度胁迫下通过两阶段培养提高小球藻高价值化合物的产量

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-07-22 DOI: 10.1016/j.bcab.2024.103315

{"title":"Enhanced production of high-value compounds from Chlorella sorokiniana by two-stage cultivation under red light and salinity stress","authors":"","doi":"10.1016/j.bcab.2024.103315","DOIUrl":"10.1016/j.bcab.2024.103315","url":null,"abstract":"<div><p>Microalgae produce valuable compounds in harsh environments, making them a promising resource for several industries. The traditional cultivation methods fail to achieve commercial viability, leading to decreased biomass production and high-value compound synthesis even under stressful conditions. To address these challenges, we conducted a two-stage cultivation to examine the impact of various LED lights and high salinity stress on the biomass and high-value compounds of the microalgae <em>Chlorella sorokiniana</em> UUIND6. When compared to the control, red light and 2-fold salinity performed best. The experimental results of the two-stage culture strategy demonstrated a substantial increase (p < 0.05) in photosynthetic pigments under red light conditions but a loss under 2-fold salinity during the second stage. Under red-light illumination, pigment concentration increased by 3.17% (chlorophyll ‘a'), 10.58% (chlorophyll ‘b'), and 62.21% for carotenoids, but was reduced by 2-fold salinity. Under 2-fold salt stress, the maximum lipid content was increased by 22.54%, followed by red LED light (15.35%). Microalgae cell size was also measured and found to be larger under red light stress (5.3 μm) and smaller under salt stress (3.3 μm). The presence of polyunsaturated fatty acids (PUFAs), monounsaturated fatty acids (MUFAs), and other molecules was verified by GC-MS and FTIR tests. Additionally, UHPLC demonstrated the considerable presence of both amino acids. The primary objective of the research was to enhance the yield of valuable compounds by improving knowledge of cultivation techniques and appropriate abiotic stressors. One such technique is the two-stage cultivation method, which enables the production of valuable compounds under diverse growth circumstances or stressors.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141736649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing bioethanol production from hassawi rice straw with Aspergillus sp. NAS51 cellulosic enzyme and in silico homology modeling 利用黑曲霉 NAS51 纤维素酶和硅同源建模优化哈萨维稻草的生物乙醇生产

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-07-19 DOI: 10.1016/j.bcab.2024.103328

{"title":"Optimizing bioethanol production from hassawi rice straw with Aspergillus sp. NAS51 cellulosic enzyme and in silico homology modeling","authors":"","doi":"10.1016/j.bcab.2024.103328","DOIUrl":"10.1016/j.bcab.2024.103328","url":null,"abstract":"<div><p>Researchers' attention has been greatly focused on the use of lignocellulosic wastes to produce bioethanol and biogas due to the depletion of non-renewable energy resources. In our effort to find a potent cellulase-producing fungal strain, the fungus NAS51 was isolated among eight isolates from a sponge collected from the Red Sea, Jeddah, and selected as it displayed the highest cellulase activity including (FP-ase, CMC-ase, and β-glucosidase enzymes) at levels of 3.13 U/ml, 2.52 U/ml, and 0.69 U/ml, respectively. The fungus was identified morphologically and genetically by sequencing its 18SrRNA gene as <em>Aspergillus</em> sp. NAS51. The cellulase activity of <em>Aspergillus</em> sp. NAS51 was optimized and maximum enzyme production was obtained at initial pH7, temp 30<sup>o</sup>C, incubation period 11 days, moisture content 70%, urea as a nitrogen source, and K2HPO4 (2 g/L). The crude cellulases from strain NAS51 were characterized, and results showed that the enzyme is stable over a wide pH range (6–10), with peak activity at pH 7.0 and 40 °C. The cellulase gene has been sequenced and the protein 3D structure was generated via in silico homology modeling. Determination of binding sites and biological annotations of the constructed protein was carried out via COACH and COFACTOR based on the I-TASSER structure prediction. To reach the maximum enzyme hydrolysis, the rice straw collected from Al-Ahsa, Kingdom of Saudi Arabia was pretreated with NaOH 1.5% to remove lignin and to enhance the saccharification process by Cellulase. The saccharified product was measured using HPLC, fermented by S. cerevisiae and the bioethanol yield produced from the fermentation was 0.454 mL ethanol/g fermentable sugars. This study indicates the potential application of fungal enzymes such as cellulase enzymes in biofuel generation and waste management.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141842065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis, characterization, and wound healing activity of alginate-based polymeric nanoparticles loaded with stingless bee honey 负载无刺蜂蜂蜜的藻酸盐基聚合物纳米粒子的合成、表征和伤口愈合活性

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-07-18 DOI: 10.1016/j.bcab.2024.103329

{"title":"Synthesis, characterization, and wound healing activity of alginate-based polymeric nanoparticles loaded with stingless bee honey","authors":"","doi":"10.1016/j.bcab.2024.103329","DOIUrl":"10.1016/j.bcab.2024.103329","url":null,"abstract":"<div><p>Nanobiotechnology could aid in developing honey-based natural wound healing agents. In the present work, stingless bee honey (SBH) loaded alginate nanoparticles (H-ALG NPs) were formulated with an ionic crosslinking method and thoroughly characterized. The average size of the H-ALG NPs was observed to be 312 ± 4.32 nm, with 0.112 ± 0.04 PDI and - 21.2 ± 0.29 mV Zeta potential. TEM and SEM imaging confirmed the presence of spherical-shaped NPs. Encapsulation efficiency and loading capacity were observed to be 84.74% and 23.12%, respectively. Further, H-ALG NPs showed dose-dependent radical scavenging activity against DPPH with IC<sub>50</sub> 23.20 compared to 35.99 mg/mL for SBH, suggesting antioxidant potential. The H-ALG NPs were also tested for cytotoxicity on a human dermal fibroblast cell line and exhibited low toxicity with a higher IC<sub>50</sub> than SBH (970.07 vs. 755.67 μg/mL; <em>P</em> < 0.001). H-ALG NPs also significantly promoted the migration of fibroblasts as indicated by scratch assay. The qPCR analysis for gene expression demonstrated that H-ALG NPs potentially promote the production of proinflammatory mediators (i.e., TNF-α, IL-6, IL-1β and IL-8) in fibroblasts, which play a crucial role in wound healing, albeit at lower levels compared to SBH. The mouse wound model confirmed that H-ALG NPs accelerated wound healing (<em>P</em> < 0.05) with improved histological characteristics. These findings highlight H-ALG NPs as a potential natural and effective therapeutic agent for wound management and advocate for further exploration of the antioxidant-based medicinal properties of SBH.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141960815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bio fabrication of microalgae mediated nanoparticles: An insight into its mechanism, characterization and applications 微藻介导纳米粒子的生物制造：深入了解其机理、特性和应用

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-07-18 DOI: 10.1016/j.bcab.2024.103330

引用次数: 0