Biocatalysis and agricultural biotechnology最新文献

{"title":"Protease inhibitor from Streptomyces pulveraceus strain VITSSAB as a potential therapeutic agent against BACE1 in Alzheimer's disease: A molecular docking and dynamics simulations study","authors":"Shatakshi Mishra ,&nbsp;B. Stany ,&nbsp;Aparana Kumari ,&nbsp;K.V. Bhaskara Rao","doi":"10.1016/j.bcab.2025.103559","DOIUrl":"10.1016/j.bcab.2025.103559","url":null,"abstract":"<div><div>Alzheimer's disease (AD) is a leading neurodegenerative disorder causing memory decline and loss of independence. BACE1 catalyzes amyloid precursor protein (APP) cleavage, forming amyloid-beta plaques. Inhibiting BACE1 is a key strategy to prevent plaque accumulation and develop AD therapies.</div><div>This study investigates high-altitude terrestrial-pigmented actinomycetes as a potential source of therapeutic compounds targeting BACE1 protease. <em>Streptomyces pulveraceus</em> VITSSAB was identified as a strong inhibitor of aspartic proteases, specifically pepsin, exhibiting a protease inhibitory activity of 66.3 ± 0.938 %. Analytical techniques, including UPLC, GC-MS, and FTIR, identified N-(Trifluoroacetyl)-N,O,O′,O″-tetrakis(trimethylsilyl)norepinephrine as the key compound responsible for the observed protease inhibition. Drug-likeness properties of N-(Trifluoroacetyl)-N,O,O′,O″-tetrakis(trimethylsilyl)norepinephrine showed favorable pharmacokinetic characteristics, reinforcing its potential as a therapeutic agent. Molecular docking studies demonstrated a strong binding affinity of −7.24 kcal/mol between this compound and BACE1 protease, indicating its efficacy in targeting the enzyme. Molecular dynamics simulations further confirmed the stability of the compound-BACE1 complex. Considering its promising characteristics, N-(Trifluoroacetyl)-N,O,O′,O″-tetrakis(trimethylsilyl)norepinephrine presents itself as a strong candidate for further development as a potential therapeutic agent targeting AD, specifically as a BACE1 inhibitor.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103559"},"PeriodicalIF":3.4,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zooplankton biomass as a promising new agent for biomedical applications","authors":"Hamdy A. Abo-Taleb ,&nbsp;Abdelrahman M. Khattab ,&nbsp;Mansour A.E. Bashar ,&nbsp;Ahmed B.M. Mehany ,&nbsp;Amer M. Abdelaziz ,&nbsp;Mohammed Abu-Elghait ,&nbsp;Mohammed M.M. El-feky","doi":"10.1016/j.bcab.2025.103560","DOIUrl":"10.1016/j.bcab.2025.103560","url":null,"abstract":"<div><div>Zooplankton biomasses are essential nutritional sources for early-stage aquatic organisms due to their high-value nutrient profiles. Recent studies highlight the potential of zooplankton mixtures and <em>Artemia franciscana</em> (Leach, 1819) biomasses as protein-rich alternatives for fish diets. The current study analyzed these biomasses for chemical composition, vitamin content, and antioxidant properties. For the first time, a zooplankton mixture was evaluated for its antimicrobial, anti-biofilm, and anticancer activities against various pathogenic microorganisms and cancer cell lines. Antimicrobial assays demonstrated that both the zooplankton mixture and <em>A. franciscana</em> biomass exhibited inhibitory effects against <em>Staphylococcus aureus</em> ATCC 6538, <em>Pseudomonas aeruginosa</em> ATCC 27853, and <em>Alternaria solani</em> RCMB 009 003, as observed through agar well diffusion. Anti-biofilm testing showed that the biomass of the zooplankton mixture effectively inhibited biofilm formation by <em>P. aeruginosa</em> ATCC 27853 and <em>S. aureus</em> ATCC 6538. Additionally, anticancer activity was assessed against three cancer cell lines: breast (MCF-7), liver (HepG-2), and colon (HCT-116). The zooplankton mixture and <em>A. franciscana</em> biomass displayed cytotoxicity across all cell lines, with IC50 values for HepG-2 at 23.47 μg mL⁻¹ and 14.48 μg mL⁻¹, respectively; for HCT-116 at 19.46 μg mL⁻¹ and 13.05 μg mL⁻¹, respectively; and for MCF-7 at 26.51 μg mL⁻¹ and 16.81 μg mL⁻¹, respectively. These findings suggest that both biomasses are promising candidates in medical applications, with potential antimicrobial and anticancer effects.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103560"},"PeriodicalIF":3.4,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive profiling and therapeutic potential of Nypa fruticans leaves: Unveiling analgesic and anti-inflammatory properties via in vivo and in silico approaches","authors":"Farhana Islam ,&nbsp;Fariya Islam Rodru ,&nbsp;Jannatul Ferdous ,&nbsp;Shopnil Akash ,&nbsp;Md Rashidur Rahman ,&nbsp;Md Nurul Islam ,&nbsp;Md Shahedur Rahman ,&nbsp;Bidduth Kumar Sarkar","doi":"10.1016/j.bcab.2025.103558","DOIUrl":"10.1016/j.bcab.2025.103558","url":null,"abstract":"<div><div>Traditionally, <em>Nypa fruticans</em> has been widely used as an herbal remedy, inspiring research into new drug development. This research aimed to explore the phytochemicals of the ethanol extract of <em>Nypa. fruticans</em> leaves (ENFL) to identify potential compounds for treating pain and inflammation. Several methods were employed to assess the i<em>n-vitro</em> antioxidant activity. The anti-inflammatory effects were calculated utilizing a formalin-induced inflammation model. Additionally, <em>in-vivo</em> analgesic activity was evaluated following an acetic acid-induced writhing test, hot plate test, and tail immersion test. Metabolites of ENFL identified via GC-MS were then subjected to computational analysis against the cyclooxygenase-2 enzyme to explore potential lead compounds. Twelve compounds were identified, with Phenol, 2,6-dimethoxy- and Epicholestanol being the most prominent. ENFL exhibited significant antioxidant activity in both the DPPH scavenging assay (IC₅₀: 19.6679 μg/mL) and the reducing power assay (RC₅₀: 0.5925 mg/mL). The <em>in-vivo</em> anti-inflammatory assay showed the highest (42.51 % and 54.33 %) suppression of edema at the fourth hour at 200 and 400 mg/kg body weight of ENFL, respectively, while indomethacin exhibited 59.48 % inhibition. Moreover, all <em>in-vivo</em> analgesics tests demonstrated a significant dose dependent effect of ENFL at the experimental doses. <em>In-silico</em> studies screened Phenol, 2,6-dimethoxy- (CID-7041), as the most active compound, exhibiting stronger binding affinities to the cyclooxygenase-2 than other compounds. Additionally, ADME/T analysis indicated that the compound exhibited favorable pharmacokinetic properties and toxicity profiles. The current <em>in-vitro</em>, in<em>-vivo,</em> and <em>in-silico</em> investigations revealed analgesics and anti-inflammatory properties of <em>Nypa fruticans</em> leaves, supporting its potential as a natural drug for treating pain and inflammation.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103558"},"PeriodicalIF":3.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Process optimization and bioflocculative insights of glycoprotein bioflocculant produced by marine bacterium Bacillus oceanisediminis LBB1","authors":"Tijo Cherian,&nbsp;Shibin Eranhottu,&nbsp;R. Mohanraju","doi":"10.1016/j.bcab.2025.103555","DOIUrl":"10.1016/j.bcab.2025.103555","url":null,"abstract":"<div><div>A type of environmentally benign and biodegradable biopolymers made by different microorganisms, bioflocculants have drawn more attention as viable substitutes for traditional chemical flocculants in the treatment of wastewater and the removal of pollutants. However, a number of obstacles prevent bioflocculants from being scaled up industrially, such as expensive substrate prices, limited manufacturing yields, and complex purifying procedures. A careful consideration must be given to the potential of microbiological contamination and the resulting health consequences linked to the use of bioflocculants. In present study, the bio-flocculative activity of <em>Bacillus oceanisediminis</em> LBB1 isolated from the gut of a finfish <em>Lutjanus biguttanus</em>, has been documented for the first time-the glycoproteinaceous bioflocculant BOB1 found to be an excellent bioflocculant with 80 % flocculating activity. The process standardization and optimization elucidated the most feasible and favourable reaction conditions ascertaining the synthesis and reaction kinetics of the bioflocculant BOB1 against kaolin clay suspension. The pattern of thermal stability of BOB1 was found to be fairly steady and stable with the maximal value reaching 94.6 % ± 1.0 % at 30 °C. The presence of various functional groups was analysed by FT-IR whereas the SEM micrographs exhibited the compact nature and fine and scattered particles of BOB-1. Hence, the creation of multipurpose flocculants based on MBF and its cooperative use with other treatment technologies are recognized as new developments for improved resource recovery and wastewater treatment.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103555"},"PeriodicalIF":3.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143628643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of natural compounds as inhibitors of Clumping Factor A in Staphylococcus aureus to combat bovine mastitis: An in-Silico approach","authors":"Y.S. Mamatha ,&nbsp;Sneha Murmu ,&nbsp;Dwijesh Chandra Mishra ,&nbsp;Mahender Kumar Singh ,&nbsp;Sunil Kumar ,&nbsp;Anu Sharma ,&nbsp;Sudhir Srivastava ,&nbsp;Krishna Kumar Chaturvedi ,&nbsp;Monika Singh ,&nbsp;Ulavappa Basavanneppa Angadi ,&nbsp;Girish Kumar Jha ,&nbsp;Shesh N. Rai","doi":"10.1016/j.bcab.2025.103556","DOIUrl":"10.1016/j.bcab.2025.103556","url":null,"abstract":"<div><div>Bovine mastitis is a significant and economically impactful disease affecting dairy cattle globally, leading to decreased milk production, higher treatment costs, compromised genetic potential, animal mortality, and substantial economic losses. Mastitis is a major bovine disease caused by various organisms, with <em>Staphylococcus aureus</em> being one of the most virulent and prevalent pathogens responsible for bovine mastitis. The overuse of antibiotics has led to antimicrobial resistance and potential adverse effects on human health, necessitating the exploration of novel antibacterial therapeutics. This study employed computational methodologies, including structure-based virtual screening, molecular docking, ADMET prediction, and molecular dynamics (MD) simulations, to identify potential ClfA inhibitors of <em>Staphylococcus aureus</em>. A library of fifty-two natural compounds was screened, all exhibiting promising binding affinities (≤−8.0 kcal/mol). The top ten compounds underwent ADMET predictions, with seven satisfying Lipinski's rule of five and displaying pharmacokinetic properties. Further analysis using MD simulations was conducted on three compounds - Diaporthalasin, Oridonin, and Salvianolic acid A. Notably, Oridonin and Salvianolic acid exhibited strong stability in MD simulations, with RMSD values below 2.5 Å and consistent hydrogen bonding interactions with ClfA. Additionally, binding free energy calculations using MM-PBSA/MM-GBSA confirmed favorable interactions, with Oridonin exhibiting binding free energy of −30.4 kcal/mol and Salvianolic acid A at −28.7 kcal/mol. These findings suggest that Oridonin and Salvianolic Acid A can be considered for preclinical trials as potential candidates for veterinary drugs targeting mastitis caused by <em>S. aureus</em>.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103556"},"PeriodicalIF":3.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143636287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of ultrasonic-assisted extraction (UAE) for phenolics and antioxidant activity from cocoa (Theobroma cacao) leaves and phytochemical profiling using GC-MS and LC-HRMS","authors":"Yusuf Andriana ,&nbsp;Muhammad Fatih Abdurrahman ,&nbsp;Pepita Haryanti ,&nbsp;Ratih Pangestuti ,&nbsp;Dedy Kurnianto ,&nbsp;Ardiba Rakhmi Sefrienda ,&nbsp;Erni Apriyati ,&nbsp;Jerry Wungkana ,&nbsp;Ashri Indriati ,&nbsp;Christina Litaay","doi":"10.1016/j.bcab.2025.103557","DOIUrl":"10.1016/j.bcab.2025.103557","url":null,"abstract":"<div><div>Cocoa (<em>Theobroma cacao</em>) leaves, often discarded as agricultural by-products, are a valuable source of phenolic compounds with strong antioxidant properties. This study aims to optimize the ultrasound-assisted extraction (UAE) process to maximize the total phenolic content (TPC) and antioxidant activity (AA) of cocoa leaves using response surface methodology (RSM) with a central composite design (CCD). Three variables—ethanol concentration (70–90%), ultrasonic irradiation time (30–60 min), and solvent-to-solid ratio (5–15 mL/g)—were evaluated for their effects on TPC and AA. The RSM quadratic model determined the optimum extraction conditions as 89.34% ethanol, 45.87 min ultrasonic irradiation, and 10.03 mL/g solvent-to-solid ratio, with predicting TPC and AA values of 129.67 mg GAE/g extract and 44.52 %, respectively. Experimental validation yielded TPC of 118.84 ± 0.83 mg GAE/g and antioxidant activity of 42.01 ± 1.14 %, aligning with predictions within a 5% error margin. Furthermore, GC-MS and LC-HRMS analyses identified key phenolics, including (+)-procyanidin B2, (−)-epicatechin, and D-(+)-catechin, alongside other compounds such as squalene. The findings demonstrate that UAE was a highly efficient method for extracting bioactive compounds from cocoa leaves, facilitating the repurposing of agricultural by-products and potentially enhancing the functional value of cocoa leaf-derived products.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103557"},"PeriodicalIF":3.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implication of indole acetic acid and biofilm production by Pseudomonas fluorescens during tomato (Solanum lycopersicum) plant interactions","authors":"Perumal Suresh ,&nbsp;Shanmugaiah Vellasamy ,&nbsp;Vellaisamy Ramamoorthy","doi":"10.1016/j.bcab.2025.103554","DOIUrl":"10.1016/j.bcab.2025.103554","url":null,"abstract":"<div><div><em>Pseudomonas fluorescens</em> plays an important role in plant growth promotion and crop protection. This research focused on indole-3-acetic acid production and biofilm formation by <em>P. fluorescens</em> strain VSMKU3054 and assessed their implications on tomato plant interactions. IAA production was optimized with different physiochemical parameters. The IAA production was at the maximum level in King's B broth (KBB) containing L-tryptophan at 0.6 g/L, glucose and beef extract as carbon and nitrogen sources, respectively, at pH 7, 37 °C and 72 h of incubation. Production of IAA by <em>P. fluorescens</em> was confirmed by TLC, FT-IR and GC-MS studies. Tomato seed treatment with <em>P. fluorescens</em> remarkably enhanced the tomato plant growth, such as root and shoot length. The research on biofilm formation at different physiochemical parameters showed that the highest level of biofilm formation was noticed in tryptic soya broth containing glucose and potassium nitrate as carbon and nitrogen sources, respectively, at pH 7, 37 °C and on the 2nd day of the incubation period. Biofilm-related traits, such as motility tests including swimming (79 ± 1.52), swarming (36 ± 1.52) and twitching (22 ± 2) can help develop an organized surface community, leading to biofilm formation. Using Green fluorescent protein-tagged <em>P. fluorescens,</em> tomato root colonization was confirmed. Thus, the results revealed that IAA-producing and biofilm-forming abilities of <em>P. fluorescens</em> strain VSMKU3054 can improve plant growth and root colonization, respectively, and these traits of <em>P. fluorescens</em> can play major roles in plant-microbe interactions.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103554"},"PeriodicalIF":3.4,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing genome editing in bovine cells: A comparative study of Cas9 variants and CRISPR delivery methods","authors":"Xiaofeng Du ,&nbsp;Alexander Quinn ,&nbsp;Timothy Mahony ,&nbsp;Di Xia ,&nbsp;Laercio R. Porto-Neto","doi":"10.1016/j.bcab.2025.103553","DOIUrl":"10.1016/j.bcab.2025.103553","url":null,"abstract":"<div><div>To assist in the establishment of an efficient CRISPR/Cas9 gene editing workflow in bovine cells, we compared the efficiency of four <em>Streptococcus pyogenes</em> Cas9 (SpCas9) nuclease variants (produced in-house or commercially) and two different Cas9/sgRNA ribonucleoprotein (RNP) delivery methods applied to Madin-Darby bovine kidney (MDBK) cells (<em>Bos taurus</em>). We targeted three genes for simple sequence deletion or modification via a single-stranded oligodeoxynucleotide donor template: the testis-determining gene <em>Sry</em> (sex-determining region on Y chromosome), germ cell-specific gene <em>Nanos2</em> (nanos C2HC-type zinc finger 2) and <em>PRLR</em> (prolactin receptor). RNPs and donor templates were delivered into cells via lipofectamine CRISPRMAX transfection or Neon electroporation. The efficiency of gene editing was determined by target-specific PCR genotyping, real-time PCR assays and next generation sequencing analyses. When targeting <em>Sry</em>, the commercial Alt-R High-Fidelity (HiFi) SpCas9 nuclease exhibited the highest deletion efficiency, followed by the in-house generated Sniper2L, HiFi SpCas9 and wild-type SpCas9. Notably, for <em>PRLR</em> and <em>Nanos2</em>, Sniper2L induced comparable editing outcomes to Alt-R HiFi SpCas9. The two delivery methods, lipofectamine CRISPRMAX transfection and Neon electroporation, demonstrated similar efficiency (60%–83%) in producing indels in all three target genes. However, Neon electroporation (5.5%–11%) was superior to CRISPRMAX lipofection (1.5%–4.8%) at inducing target-specific sequence knock-ins. Strategies to inhibit NHEJ repair and/or enhance HDR may be necessary to improve HDR efficiency in these bovine cells. These findings provide valuable insights for improving gene editing outcomes in bovines and may assist in accelerating the widespread application of genome editing technology in large animals.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103553"},"PeriodicalIF":3.4,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-production of bioethanol and xylitol from steam-exploded bamboo using the newly isolated yeast strain Sugiyamaella marilandica HYSM006","authors":"Luis Hoppert,&nbsp;Thomas Brune,&nbsp;Daniel Einfalt","doi":"10.1016/j.bcab.2025.103551","DOIUrl":"10.1016/j.bcab.2025.103551","url":null,"abstract":"<div><div>This study investigated a novel yeast strain, <em>Sugiyamaella marilandica</em> HYSM006, for its potential in bioethanol and xylitol production from lignocellulosic biomass. HYSM006 demonstrated efficient glucose conversion to ethanol under both anaerobic and aerobic conditions in synthetic media, achieving up to 49.7 g L⁻¹ ethanol from 100 g L⁻¹ glucose. Aerobic cultivation in 100 g L⁻¹ xylose yielded 40.7 g L⁻¹ xylitol and 16.5 g L⁻¹ ethanol. The strain demonstrated very low ethanol consumption after glucose exhaustion and exhibited good osmotic stress tolerance up to a concentration of 230 g L⁻¹ glucose and 61.6 g L⁻¹ ethanol. After adaptation to lignocellulosic hydrolysates the strain was able to utilize bamboo hydrolysate at a solid loading of 20 % w w⁻¹, achieving 19.2 g L⁻¹ ethanol and 3.4 g L⁻¹ xylitol. The results underscore <em>Sugiyamaella marilandica</em> HYSM006's potential for biotechnical application, showcasing its ability to effectively utilize glucose and xylose. Despite its potential, further research is needed to enhance the strain's yield and ethanol tolerance for commercial-scale applications.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103551"},"PeriodicalIF":3.4,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143591486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of black Aspergillus-based soil amendments on 137Cs uptake by rice grown in Fukushima-contaminated soils","authors":"Salem Djedidi ,&nbsp;Youhei Yamagata ,&nbsp;Takuya Ban ,&nbsp;Taiichiro Ookawa ,&nbsp;Naoko Ohkama-Ohtsu ,&nbsp;Tadashi Yokoyama","doi":"10.1016/j.bcab.2025.103552","DOIUrl":"10.1016/j.bcab.2025.103552","url":null,"abstract":"<div><div>Black <em>Aspergillus</em> species produce several metabolites, including organic acids, that can facilitate the release of soil-attached elements. In Fukushima soils, radiocesium has been observed to adhere to soil particles. We hypothesized that <em>Aspergillus</em>-derived organic acids may function as weathering agents, potentially affecting the phyto-availability of radiocesium in soils. Therefore, new soil amendments, comprising fermented wheat bran by different <em>Aspergillus</em> strains, were formulated and evaluated on Fukushima soils to assess their effects on rice growth and radiocesium uptake. Two pot experiments were conducted. The initial experiment focused on evaluating the fermentation product of <em>A. saitoi</em>, while the subsequent experiment assessed the fermentation products of seven strains belonging to <em>A. luchuensis</em> (one strain), <em>A. niger</em> (five strains), and <em>A. saitoi</em> (one strain) under laboratory-controlled and open-air cultivation conditions in Fukushima. Both experiments examinated the effects of amendments on growth and radiocesium uptake by rice grown in different Fukushima soils.</div><div>In the first experiment, <em>A. saitoi</em> amendment considerably enhanced shoot biomass and radiocesium quantity in TAT-26 rice line, by nearly 100% and 400%, respectively. In the second experiment, amendments from different <em>Aspergillus</em> strains revealed positive and negative effects on growth and radiocesium uptake by TAT-26. These effects varied depending on soil types and climatic conditions in which plants were cultivated. In the brown forest soil, results showed that amendment-induced soil acidification may increase radiocesium phyto-availability. TAT-26 showed a considerable ability to accumulate radiocesium in its above-ground biomass, making it a candidate accumulator plant. However, this feature must be verified under field conditions.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103552"},"PeriodicalIF":3.4,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}