Biocatalysis and agricultural biotechnology最新文献

{"title":"Silver nanoparticles for control of foliar pathogens in passion fruit (Passiflora edulis) crops","authors":"Natália Deniz Brito ,&nbsp;Rafael José Vilela de Oliveira ,&nbsp;Felipe Leon Nascimento de Sousa ,&nbsp;Ana Júlia Silva Rocha ,&nbsp;Maria Eduarda Almeida Matos ,&nbsp;Laura Lima Turani ,&nbsp;Raphael Ferreira Queiroz ,&nbsp;Bianca Galúcio Pereira Araújo ,&nbsp;Giovanna Machado ,&nbsp;Quelmo Silva de Novaes","doi":"10.1016/j.bcab.2025.103717","DOIUrl":"10.1016/j.bcab.2025.103717","url":null,"abstract":"<div><div>Sustainable management of phytopathogens has driven the use of metallic nanoparticles due to their high antimicrobial efficacy, stability and environmental compatibility. In this work, we investigate the antimicrobial action of silver nanoparticles mycosynthesized against pathogens of yellow passion fruits (<em>Passiflora edulis</em>). Pathogen selection involved field sampling, isolation, and reinfection assays, confirming the presence of <em>Cladosporium cladosporioides</em> and <em>Colletotrichum theobromicola</em>. as well as investigated the bacterium <em>Xanthomonas axonopodis</em> pv. <em>passiflorae</em>. The AgNPs were mycosynthesized using the endophytic extract of <em>Aspergillus brasiliensis</em> and co-stabilized by carboxymethyl cellulose, and characterized by structural, morphological and interfaces techniques, exhibiting important physicochemical characteristics as diameter of 18.78 nm, and zeta potential of −23.41 mV. The <em>in vitro</em> assays demonstrated the inhibitory effect of AgNPs for mycelial growth of <em>C. cladosporioides</em> and <em>C. theobromicola,</em> with dose-dependent response, reaching inhibition exceeding 80 % for 60–100 μg mL⁻¹. Concentrations above 20 μg mL⁻¹ effectively inhibited the conidial germination. Electron microscopies revealed structural damage to hyphae and cell wall fungi. For <em>Xanthomonas axonopodis</em> pv. <em>passiflorae</em>, the minimum inhibitory concentration (MIC) was 20 μg mL⁻¹, and the minimum bactericidal concentration (MBC) was 60 μg mL⁻¹. These results underscore the potential of mycosynthesized nanoparticles as antimicrobial agents, presenting a promising alternative for the management of passion fruit diseases. The importance of <em>in vitro</em> studies is also highlighted as a methodology for understanding the action of nanoparticles under controlled conditions, allowing the pre-selection of candidates and the definition of boundary conditions, improving the design of <em>in vivo</em> experiments.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103717"},"PeriodicalIF":3.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144878891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization, simulation and techno-economic studies of microwave-assisted oil extraction from Calophyllum inophyllum L. kernels","authors":"Phonsan Saetiao ,&nbsp;Napaphat Kongrit ,&nbsp;Jakkrapong Jitjamnong ,&nbsp;Nonlapan Khantikulanon","doi":"10.1016/j.bcab.2025.103709","DOIUrl":"10.1016/j.bcab.2025.103709","url":null,"abstract":"<div><div>This study focused on optimizing and evaluating the efficiency of oil extraction from <em>Calophyllum inophyllum</em> L. kernels using hexane as the solvent through microwave-assisted extraction. The process was systematically studied using a central composite design under response surface methodology to determine the influence of three key parameters solvent: seed kernel (v/w) ratio, extraction temperature, and extraction time. Optimal conditions were identified as a solvent: kernel (v/w) ratio of 15.92 mL/g, a temperature of 39.95 °C, and an extraction time of 26.64 min, achieving a maximum oil yield of 68.76 %. Analysis of variance confirmed that the solvent: kernel (v/w) ratio and extraction time were significant factors affecting the oil yield. Chemical profiling of the extracted oil via gas chromatography-mass spectrometry revealed oleic acid (C18:1) as the predominant fatty acid, emphasizing the oil potential for various industrial applications. Kinetic studies established that the extraction process followed a first-order reaction with an activation energy of 44.62 kJ/mol. To evaluate scalability, a plant simulation was developed using the Aspen Plus software, with economic modeling performed using Aspen Process Economic Analyzer, and revealed that the oil selling price was the most critical parameter influencing profitability. Financial assessment indicated the process viability, with a net present value of $4,246,890, an internal rate of return of 15.65 %, and a payback period of 8.01 years. These findings highlight the feasibility and economic potential of MAE for <em>Calophyllum inophyllum</em> L. oil (CLO) extraction on an industrial scale.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103709"},"PeriodicalIF":3.8,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing pyrene biodegradation via RSM-based optimization and characterization of catechol 1, 2-dioxygenase and 2, 3-dioxygenase in Acinetobacter baumannii BJ5 strain","authors":"Bineypreet Kaur ,&nbsp;Pushti Verma ,&nbsp;Shailendra Kumar Arya ,&nbsp;Jaspreet Kaur ,&nbsp;Satwant Kaur Shahi","doi":"10.1016/j.bcab.2025.103708","DOIUrl":"10.1016/j.bcab.2025.103708","url":null,"abstract":"<div><div>Catechol 1, 2-dioxygenase (C12O) and catechol 2, 3-dioxygenase (C23O) are key enzymes involved in the biodegradation pathways, catalyzing the breakdown of aromatic pollutants including polycyclic aromatic hydrocarbons (PAHs) in environmental systems. The current study employed Response Surface Methodology (RSM) with a Box-Behnken Design (BBD) to optimize key biochemical parameters, namely pH, temperature, and substrate concentration, affecting the activity of these dioxygenases from <em>Acinetobacter baumannii</em> BJ5, in the biodegradation of the high molecular weight polycyclic aromatic hydrocarbon (HMW PAH), pyrene. Optimization was performed within pH range of 6.4–8.4 for C12O and 6.0 to 8.0 for C23O, temperature range of 30 °C–40 °C for C12O and 25 °C–35 °C for C23O, and substrate concentration from 2.0 to 6.0 mM for C12O and 1.5–5.5 mM for C23O. The optimal conditions determined were 7.5 and 7.0 (pH), 35 °C and 30 °C (temperature), and 6.0 mM and 4.0 mM (Substrate concentration) for C12O and C23O, respectively. Partial purification of C12O increased purity 1.10-fold with 57.83 % yield and raised specific activity from 75.50 to 87.33 μmol/min/mg. The findings underline the significant potential of dioxygenases in degradation of recalcitrant PAHs, thereby supporting practical deployment in contaminated soil and water systems. <strong>This investigation constitutes one of the first efforts to employ RSM for optimizing PAH degrading dioxygenases in a gram-negative strain, <em>A. baumannii</em> BJ5 with respect to degradation of HMW PAH, pyrene and</strong> provide valuable insights toward developing enzyme based strategies for the sustainable management of persistent industrial pollutants.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103708"},"PeriodicalIF":3.8,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellulose-producing acetic acid bacteria from corozo fruit (Bactris guineensis): isolation and characterization","authors":"Armando Ricardo-Herazo ,&nbsp;Robin Zuluaga ,&nbsp;Jorge Figueroa-Flórez ,&nbsp;José Severiche-Castro ,&nbsp;Yaremis Meriño-Cabrera","doi":"10.1016/j.bcab.2025.103707","DOIUrl":"10.1016/j.bcab.2025.103707","url":null,"abstract":"<div><div>Acetic acid bacteria (AAB), members of the <em>Acetobacteraceae</em> family, are known for their ability to synthesize bacterial cellulose (BC), a biopolymer of growing interest in the food, biomedical, and materials industries. Compared to plant-derived cellulose, BC offers higher purity and superior physicochemical properties; however, its large-scale production remains limited by the high cost of cultivation media and nutritional demands of AAB. In this study, corozo fruit (<em>Bactris guineensis</em>) fermentations were explored as a natural source of BC-producing bacteria. Forty-five bacterial isolates were obtained and biochemically characterized, of which five demonstrated cellulose production in Hestrin–Schramm (HS) medium under static conditions. Strain A03 showed the highest yield (9 g/L), comparable to the reference strain <em>Komagataeibacter xylinus</em>, followed by strains A05, A12, and A40 with intermediate yields, and strain A25 with the lowest. Molecular identification through 16S rRNA gene sequencing revealed high similarity to <em>Komagataeibacter hansenii</em>. The biopolymers were characterized using FTIR spectroscopy, scanning electron microscopy (SEM), and thermogravimetric analysis (TGA), confirming the structural and thermal features typical of BC, with strain-dependent differences in crystallinity, fiber morphology, and hydrogen bonding. These native <em>K. hansenii</em> strains represent promising candidates for the development of cost-effective, sustainable BC production strategies and offer potential for integration into circular bioeconomy models based on regional biodiversity.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103707"},"PeriodicalIF":3.8,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144723191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the antibacterial and antifungal activities of the extracts of Aerides multiflora Roxb. a native medicinal orchid","authors":"Mst. Naznin Ayesha ,&nbsp;Sreeramanan Subramaniam ,&nbsp;S.M. Shahinul Islam","doi":"10.1016/j.bcab.2025.103706","DOIUrl":"10.1016/j.bcab.2025.103706","url":null,"abstract":"<div><div>The present study was conducted to evaluate the antimicrobial activity of <em>Aerides multiflora</em>, an important epiphytic medicinal orchid in Bangladesh. Extracts from whole plants and seed pods were collected using methanol, chloroform, petroleum ether, and aqueous extract to testify their antibacterial activities against five bacterial strains, namely <em>Bacillus cereus, E. coli, Staphylococcus aureus, Pseudomonas</em> sp., and <em>Shigella boydii</em>. Four pathogenic fungi, namely <em>Rhizopus oryzae</em>, <em>Aspergillus flavus, Aspergillus niger</em> and <em>Fusarium oxysporum</em> were also tested. The antibacterial activities were evaluated using the disc diffusion method, and the antifungal activities were tested using the poison feeding technique. The whole plant methanol extract was superior to the other solvent extracts against the bacteria and fungi tested. The highest zone of inhibition by the whole plant methanol extract was recorded against <em>Staphylococcus aureus</em> (14.33 ± 0.19) and the highest percentage inhibition of mycelial growth was observed against <em>A. flavus</em> (62.19 ± 0.57). The value of minimum inhibitory concentration of the extracts was also analyzed. The whole plant methanol extract gave the lowest MIC value against both the tested bacteria and fungi. The results could be used for the isolation and characterization of the novel phytochemical effects on various infectious diseases, especially in view of the emergence of drug-resistant microorganisms such as antimicrobial and antifungal agents.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103706"},"PeriodicalIF":3.8,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144723204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insecticide degradation potential of aphid associated bacteria","authors":"Mahesh S. Yandigeri ,&nbsp;Sampada N. ,&nbsp;Manoj Kumar Solanki ,&nbsp;Sanjay Yalashetti ,&nbsp;M. Mohan ,&nbsp;G. Sivakumar ,&nbsp;B.S. Manjunatha ,&nbsp;S.N. Sushil","doi":"10.1016/j.bcab.2025.103703","DOIUrl":"10.1016/j.bcab.2025.103703","url":null,"abstract":"<div><div>Microorganisms that are naturally present in the insect gut play a crucial role in regulating physiological and metabolic functions, ultimately enhancing the host's fitness and adaptability to challenging environments. This includes detoxification processes that break down harmful substances and xenobiotics, such as insecticides. This study investigated the capacity of bacteria associated with aphids to degrade imidacloprid residues in agricultural soils, a crucial factor in mitigating environmental pollution. Two bacterial strains, <em>Exiguobacterium indicum</em> MPB-2 and <em>Pseudomonas hibiscicola</em> CCF 2-2, were isolated from the midgut of aphids and assessed for their ability to degrade imidacloprid in both sterile and non-sterile organic soils. <em>E. indicum</em> MPB-2 exhibited superior imidacloprid degradation efficiency, realizing 45.65 % imidacloprid breakdown in sterile soil and 33.45 % in non-sterile soil. A field experiment within a cabbage cropping system further validated the efficacy of <em>E. indicum</em> MPB-2 and <em>P. hibiscicola</em> CCF 2-2, which reduced imidacloprid residues by 31.4 % and 31.0 %, respectively. In multi-location trials, consistent degradation performance was observed across all test locations, with <em>E. indicum</em> MPB-2 outperforming <em>P. hibiscicola</em> CCF 2-2. These findings suggest that these bacterial strains facilitated imidacloprid degradation and possess the metabolic flexibility to utilize insecticides as their primary carbon and nitrogen sources. Accordingly, their presence in the aphid gut could enhance host resilience while contributing to bioremediation efforts in agricultural ecosystems. This study highlighted the potential application of aphid-associated bacteria in reducing the toxicity of imidacloprid pesticide and promoting sustainable agriculture.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103703"},"PeriodicalIF":3.8,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144723190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heavy metals removal and reduction of heavy metal phytotoxicity by Arthrobacter, Bacillus, and Pseudomonas strains harboring genes encoding metal resistance","authors":"Muhammad Tahir ,&nbsp;Muhammad Nasir ,&nbsp;Tahir Naqqash ,&nbsp;Muhammad Shahid ,&nbsp;Abu Bakr Umer Farooq ,&nbsp;Muhammad Akram ,&nbsp;Muhammad Arshad ,&nbsp;Muhammad Kamran ,&nbsp;Urooj Khan","doi":"10.1016/j.bcab.2025.103705","DOIUrl":"10.1016/j.bcab.2025.103705","url":null,"abstract":"<div><div>An experiment was conducted to assess the potential of bacteria isolated from wastewater-irrigated soils to remediate heavy metals and mitigate heavy metal-induced phytotoxicity in wheat. Four bacterial isolates, i.e., M50-3/1, wwCd-5/1, wwCd-5/2, and wwCd-5/3 were isolated from wastewater-irrigated soils and tested for metal tolerance by MIC assay. Metal removal efficiency was evaluated in a mini-pilot assay involving both wastewater and contaminated soil. The bacterial isolates showed tolerance to all the levels (50–2000 mg L⁻¹) of the heavy metals (Cd, Pb, and Zn). Whereas, for As the MIC values of the isolates M50-3/1, wwCd-5/2, and wwCd-5/3 were 100, 2000, and 500 mg L⁻¹, respectively. All the bacterial isolates significantly solubilized higher amounts of phosphate, produced IAA and antioxidants (APX, CAT, and POX) in the growth medium supplemented with 500 mg L⁻¹ each of As, Cd, Pb, Zn, individually and in combined application as compared to control. In the assay, the lowest amounts of As (45.0 μg L⁻¹), Cd (0.21 μg L⁻¹), Pb (0.77 μg L⁻¹), and Zn (0.3 μg L⁻¹) were detected in the supernatant of treatment T4. The isolates wwCd-5/1, wwCd-5/2, wwCd-5/3, and M50-3/1 were identified as <em>Arthrobacter</em> sp., <em>Bacillus thuringiensis</em>, <em>Bacillus aequororis</em>, and <em>Pseudomonas putida</em>, respectively, through <em>16S rRNA</em> sequencing. The amplified sequences of heavy metal resistance-encoding genes (CzcA, CzcD, and pbrA) from four isolates revealed significant sequence homology with those found in <em>Bacillus</em> and <em>Pseudomonas</em> strains. The application of metal-tolerant bacteria improved plant growth and reduced shoot metal contents and phytotoxicity under metal stress of 500 mg kg⁻¹.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103705"},"PeriodicalIF":3.8,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144723160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoemulsions as advanced delivery systems of bioactive compounds for sustainable food preservation applications","authors":"Nikita ,&nbsp;Shekhar Agnihotri","doi":"10.1016/j.bcab.2025.103702","DOIUrl":"10.1016/j.bcab.2025.103702","url":null,"abstract":"<div><div>Nanoemulsions have emerged as advanced delivery systems for bioactive compounds, offering significant potential in sustainable food preservation. These colloidal systems, composed of nano-sized droplets (20–200 nm), exhibit exceptional physical stability, enabling effective encapsulation, protection and controlled release of lipophilic bioactive compounds such as essential oils, natural antimicrobials and antioxidants. Their distinctive physicochemical properties, including high surface area, optical transparency and improved solubility, effectively address challenges related to the stability and functionality of bioactives under diverse environmental conditions. Additionally, nanoemulsions act as natural barriers against oxidation and microbial spoilage, thus preserving food quality and extending the shelf life of perishable products. This review examines the formulation techniques of nanoemulsions, including high-energy and low-energy approaches, along with their composition, characterization and the factors influencing their properties and performance. The review also highlights the role of nanoemulsions in enhancing the antimicrobial and antioxidant efficacy of natural compounds, demonstrating their significant contribution to reducing food spoilage. Overall, nanoemulsions offer a sustainable, multifunctional platform for reducing food spoilage and extending product longevity, aligning with the global shift toward eco-friendly food preservation technologies.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103702"},"PeriodicalIF":3.8,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144750765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response surface methodology-based optimised ultrasound-assisted extraction of phenolics from Solanum lasiocarpum Dunal (terung asam) fruit: Anti-diabetic evaluation and ADMET study","authors":"Aniza Saini ,&nbsp;Mohammad Amil Zulhilmi Benjamin ,&nbsp;Muhammad Daniel Eazzat Mohd Rosdan ,&nbsp;Mohamad Norisham Mohamad Rosdi ,&nbsp;Mohd Azrie Awang","doi":"10.1016/j.bcab.2025.103704","DOIUrl":"10.1016/j.bcab.2025.103704","url":null,"abstract":"<div><div><em>Solanum lasiocarpum</em> Dunal, known as ‘terung asam,’ is an indigenous fruit-vegetable widely cultivated on Borneo Island, particularly in Sarawak (Malaysia) and Kalimantan (Indonesia). This study optimised the ultrasound-assisted extraction of <em>S. lasiocarpum</em> fruit extract (SLFE) using response surface methodology (RSM) and evaluated its anti-diabetic activity. Initial screening with a one-factor-at-a-time (OFAT) approach assessed solvent extraction, extraction time, ultrasonic amplitude, and solid-to-solvent ratio. These parameters were further refined using RSM with a central composite design to optimise crude extract yield (CEY), total phenolic content (TPC), and chlorogenic acid (CGA). Anti-diabetic activity was assessed via <em>in silico</em> molecular docking and <em>in vitro</em> α-glucosidase inhibition. Optimal conditions were an extraction time of 8.93 min, ultrasonic amplitude of 57.00 %, and a solid-to-solvent ratio of 1:29.68 g/mL, resulting in CEY (45.32 ± 0.67 %), TPC (28.22 ± 0.86 mg GAE/g), and CGA (1.78 ± 0.12 mg/g). <em>In silico</em>, CGA demonstrated a higher molecular docking vina score (−7.1 kcal/mol) than acarbose (−6.5 kcal/mol), while <em>in vitro</em>, SLFE exhibited an IC₅₀ of 54.82 ± 0.08 μg/mL, compared to acarbose (9.47 ± 0.13 μg/mL) and CGA (8.15 ± 0.22 μg/mL). These findings establish SLFE as a valuable source of phenolic compounds with significant anti-diabetic activity, suggesting its potential as a food ingredient and dietary supplement.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103704"},"PeriodicalIF":3.8,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144723203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive potential of date palm by-products (Phoenix dactylifera L.): Optimized extraction and antimicrobial activity against veterinary pathogens","authors":"Nahla Mejri ,&nbsp;Mariem Ben Jemaa ,&nbsp;Hanen Falleh ,&nbsp;Majdi Hammami ,&nbsp;Sylvain Guyot ,&nbsp;Renato B. Pereira ,&nbsp;Hélène Sotin ,&nbsp;Fabrizio Ceciliani ,&nbsp;Latifa Abdennebi-Najar ,&nbsp;Wided Megdiche-Ksouri","doi":"10.1016/j.bcab.2025.103701","DOIUrl":"10.1016/j.bcab.2025.103701","url":null,"abstract":"<div><div>The sustainable utilization of agricultural by-products is essential for environmental and economic benefits. This study uses response surface methodology to extract bioactive compounds from date palm by-products (seeds, perianths, spikelets). Extracts' analyses included total phenolic content, antioxidant activity, antimicrobial activity, and phenolic profiles <em>via</em> UHPLC-UV-MS. Optimal extraction conditions were identified as follows: for seeds, 5 min at 20 °C with 60 % ethanol; for perianths, 4 min at 17 °C with 61 % ethanol; and for spikelets, 4.5 min at 50 °C with 60 % ethanol. Mass spectrometry analysis revealed the presence of 28 secondary metabolites, including phenolic acids, flavonoids, glycosides, and sulfated flavonoids. The extracts showed significant antioxidant activity, with the perianth extract exhibiting the highest DPPH inhibition percentage. The extracts’ antimicrobial activity was tested against nine pathogenic bacteria. The spikelet extract showed the strongest inhibition of <em>E. coli</em>, while the perianth extract effectively inhibited <em>S. marcescens</em>, <em>S. typhimurium</em>, and <em>E. faecalis</em>. Minimum inhibitory concentrations for <em>S. aureus</em> were 0.1 mg/mL for the perianth extract, 0.5 mg/mL for the spikelet extract, and 2.5 mg/mL for the seed extract. Besides optimizing the extraction procedure for phenolic compounds from date palm by-products, the present study demonstrated their antioxidant and antimicrobial properties. These findings underscore the significance of date palm by-products as valuable resources for developing functional products, thereby contributing to sustainability and a circular economy.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103701"},"PeriodicalIF":3.4,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}