Biocatalysis and agricultural biotechnology最新文献

{"title":"Exploring freeze-thawed cellulose-based hydrogel from corn Cob: Physicochemical properties, antibacterial activity, and cytotoxicity assay","authors":"Marathur Rodhiyah ,&nbsp;Nur'aini Nafisah ,&nbsp;William Xaveriano Waresindo ,&nbsp;Halida Rahmi Luthfianti ,&nbsp;Nabila Asy Syifa ,&nbsp;Dian Ahmad Hapidin ,&nbsp;Dhewa Edikresnha ,&nbsp;Khairurrijal Khairurrijal","doi":"10.1016/j.bcab.2025.103629","DOIUrl":"10.1016/j.bcab.2025.103629","url":null,"abstract":"<div><div>Corn cobs, an abundant yet underutilized agricultural residue in Indonesia, offer a promising source of cellulose for the development of sustainable biomaterials. This study focuses on the extraction of cellulose from corn cobs and its subsequent application in hydrogel fabrication for potential biomedical use. The cellulose was isolated through sequential delignification, bleaching, and acid hydrolysis, yielding a cellulose content of 61.80 %. Hydrogels were synthesized via a freeze–thaw method using varying concentrations of NaOH (2–7 %) to investigate the influence of alkalinity on structural and functional properties. Among all variations, the hydrogel prepared with 3 % NaOH demonstrated the most favorable characteristics, including the highest swelling ratio, enhanced compressive strength, and improved thermal stability. Scanning electron microscopy revealed a uniform, interconnected porous structure, while FTIR spectroscopy confirmed the retention of functional groups associated with cellulose. X-ray diffraction analysis showed a shift from the semi-crystalline nature of the cellulose powder (crystallinity index: 61.48 %) to a predominantly amorphous structure in the hydrogel. Differential scanning calorimetry supported this finding, revealing a significant reduction in heat of fusion. The hydrogel also exhibited antibacterial activity against <em>Staphylococcus aureus</em> and <em>Pseudomonas aeruginosa</em>, while cytotoxicity assays indicated IC₅₀ values above 900 μg/mL, confirming its biocompatibility. These results highlight the potential of corn cob-derived cellulose hydrogels as safe, bio-based materials suitable for biomedical applications, aligning with green bioprocessing and agricultural biotechnology initiatives.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103629"},"PeriodicalIF":3.4,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Germination response of South African cannabis landraces to accelerated aging: Implications for seed storage","authors":"Sabeliwe Langa ,&nbsp;Lembe Samukelo Magwaza ,&nbsp;Asanda Mditshwa ,&nbsp;Samson Zeray Tesfay","doi":"10.1016/j.bcab.2025.103631","DOIUrl":"10.1016/j.bcab.2025.103631","url":null,"abstract":"<div><div>This study investigated the impact of accelerated aging on seed germination in five South African cannabis landraces: Ladysmith Ugwayi wesiZulu (‘L1’) and Iswazi (‘L2’), Bergville Natal (‘B2’) and Ugwayi wesiZulu (‘B1’), and Msinga Ugwayi wesiZulu (M1). Seeds were subjected to aging at 42 °C for 0 (control), 24, 48, 72, 96, and 120 h. Seed viability assessed using a tetrazolium chloride (TTC) test, electrolyte conductivity, pH, and water activity were measured over five days. Germination tests were conducted at 30/25 °C in Petri dishes in the laboratory and validated in a greenhouse, with daily recording of germination percentage (GP), germination rate index (GRI), and coefficient of velocity of germination (CVG). Accelerated aging significantly (p &lt; 0.05) reduced seed viability, GP, GRI, and CVG while increasing electrolyte leakage and water activity. Landrace responses varied with ‘B1’ having maintained the highest viability (100 % unaged, 94.41 % after 120 h), whereas ‘M1’ showed the lowest (70.63 % after aging). Electrolyte conductivity was highest in ‘M1’ (393.2 μS cm⁻¹ g⁻¹ after 120 h), while ‘L2’ exhibited the highest water activity (0.724 after 120 h). Germination declined significantly under prolonged aging, with ‘L2’ and ‘B2’ exhibiting over 50 % reductions in GP. The findings demonstrate that accelerated aging impairs seed quality through electrolyte leakage and moisture uptake demonstrated by water activity, with landrace-dependent sensitivity. These results emphasize the need for controlled storage to preserve cannabis seed viability and germination performance, particularly under conditions of elevated temperature and humidity common to many smallholder farming systems.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103631"},"PeriodicalIF":3.4,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144230201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A slow-release urea prepared via sandwich with degradable chitosan: Formulation and release mechanisms","authors":"Retno Ariadi Lusiana ,&nbsp;Sari Edi Cahyaningrum ,&nbsp;Taufik Abdillah Natsir ,&nbsp;Lisna Efiyanti ,&nbsp;Hasan Muhtar","doi":"10.1016/j.bcab.2025.103626","DOIUrl":"10.1016/j.bcab.2025.103626","url":null,"abstract":"<div><div>To reduce environmental pollution due to excessive use of urea fertilizer, a new fertilizer formulation has been created, made from urea combined with sulfosuccinate crosslinked chitosan (CS), polyvinyl pyrrolidone (PVP), and Ca²⁺, for application as slow-release urea. The formulation is made by coating urea using Chitosan-sulfosuccinate-PVP (CSP) and Ca²⁺ to form a mesh material. Based on functional group test data, it is indicated that the interaction between urea and CSP occurs through the formation of hydrogen bonds, where urea is trapped in the middle of the net structure covered by CSP and Ca²⁺ acts as an outer coating. The layer will act to protect water contact with the urea so that dissolution is controlled, and the urea does not decompose quickly into the environment. This system facilitates the regulated solubilization of urea over a duration of 30 days, employing a slow-release mechanism that around 30 ppm/day under conditions of pH 7 at ambient temperature. Based on the SEM morphological traces, urea is released through a small tunnel created by the slow penetration of water from the outside to the inside of the membrane.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103626"},"PeriodicalIF":3.4,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144205030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upcycling corn cropping waste by nanocellulose production","authors":"David Fernando dos Santos ,&nbsp;Rafaela R. Ferreira ,&nbsp;Alana Gabrieli Souza ,&nbsp;Gabriela Caroline Lenhani ,&nbsp;Vinícius Gonçalves Deon ,&nbsp;Derval S. Rosa ,&nbsp;Vânia Zanella Pinto","doi":"10.1016/j.bcab.2025.103624","DOIUrl":"10.1016/j.bcab.2025.103624","url":null,"abstract":"<div><div>Waste revaluation is a topic of interest in science and has been widely explored to produce nanocellulose, showing the unlimited application potential and production growth. This work evaluates the nanocellulose production from high cellulose content corn crop waste (stalk, cob, and husk). To produce nanocellulose, chemical isolation, and bleaching were used for corn wastes, followed by a mechanical ball milling fibrillation at three different periods (6h, 9h, and 12h). Nanocellulose was evaluated using dynamic light scattering, zeta potential, infrared (FTIR) and Raman spectrometry, X-ray diffraction (XRD), and transmission electron microscopy (TEM). Ball milling fibrillation time increases the crystallinity index (CI) in all corn waste cellulose samples. Also, nanometric-scale sizes (107–335 nm of hydrodynamic radius) and a certain dissolution stability were achieved due to their chemical constitution. The morphology shows nanofibrils for each sample, with diameters ranging from 2.03 to 16.73 nm after 12h of ball milling. This study shows an efficient green method to obtain nanocellulose from corn crop wastes with potential for applications in several fields.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103624"},"PeriodicalIF":3.4,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential use of rhizobial exopolysaccharides to protect wheat (Triticum aestivum) germination under salt stress","authors":"Saliha Gharbi ,&nbsp;Anass Elyemlahi ,&nbsp;Hanae H’daidane ,&nbsp;Hanane Bakrim ,&nbsp;Mounir Hassani Zerrouk ,&nbsp;Amin Laglaoui ,&nbsp;Mostafa Lamhamdi ,&nbsp;Abdelhay Arakrak ,&nbsp;Ouiam El Galiou","doi":"10.1016/j.bcab.2025.103627","DOIUrl":"10.1016/j.bcab.2025.103627","url":null,"abstract":"<div><div>Salinity is a major limiting factor in agricultural productivity. This study evaluates the effect of two exopolysaccharides (EPS), produced by <em>Bradyrhizobium cytisi</em> (EPS_B21) and <em>Rhizobium sullae</em> (EPS_BG15), on the tolerance of wheat (<em>Triticum aestivum</em>) to salt stress. Treatments included a control, NaCl (8 g.L⁻¹), EPS alone (0.5 and 5 mg/mL) and EPS + NaCl combinations. EPS were extracted by ethanol precipitation. <em>R. sullae</em> and <em>B. cytisi</em> yielded 45 g.L⁻¹ and 37 g.L⁻¹ respectively. The extracts showed antioxidant activity, with EPS_BG15 achieving 69.15 % DPPH inhibition at 9 mg/mL, 26.32 % higher than EPS_B21. On the other hand, the FRAP assay revealed a better iron reduction capacity for EPS_B21 (IC₅₀ = 20.74 mg/mL vs. 59.36 mg/mL for EPS_BG15). Under salt stress, the addition of EPS at 0.5 mg/mL improved germination (89.33 %) compared with NaCl alone (70.66 %). However, fresh and dry weights remained lower than those of the control (0.25 g and 0.02 g), particularly for the fresh weight with EPS_B21+NaCl (0.09 g), despite the improved germination rate. EPS_BG15 treatment maintained high levels of chlorophyll <em>a</em> (37.94 μg/g), chlorophyll <em>b</em> (21.52 μg/g) and carotenoids (9.34 μg/g), while reducing NaCl-induced proline accumulation (182.28 %). Antioxidant enzyme activity varied according to the treatments: APX was activated by EPS_B21(5 mg/mL), with activity reaching around 1.035 U.mg⁻¹ of protein, compared with NaCl treatment, while CAT and POD showed contrasting responses depending on the doses and combinations applied. These observations reinforce the interest of EPS, particularly those from <em>R. sullae</em>, as natural biofertilizers capable of mitigating salt stress effects.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103627"},"PeriodicalIF":3.4,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical profiling and evaluation of antimicrobial, cytotoxic, wound-healing, and photocatalytic properties of green-synthesized silver nanoparticles using Verbascum splendidum","authors":"Ömer Hazman ,&nbsp;Ibrahim Erol ,&nbsp;Gofur Khamidov","doi":"10.1016/j.bcab.2025.103625","DOIUrl":"10.1016/j.bcab.2025.103625","url":null,"abstract":"<div><div>Recent trends in green chemistry highlight the increasing importance of environmentally friendly nanoparticle synthesis. Recent studies highlight the environmental advantages and industrial potential of biogenic synthesis pathways. Silver nanoparticle (AgNPs) production was carried out using <em>V. splendidum</em> aqueous extract (VSE) by green synthesis, which is an environmentally friendly, cheap, and easily applicable method. In the content of VSE determined by the LC-MS/MS system, it was determined that the species responsible for the stabilization of AgNPs were phenolic acid and flavonoids. The formation of AgNPs was understood from the surface plasma resonance peak at 402 nm and the peaks showing the face-centered cubic silver crystalline structure in X-ray powder diffraction analysis. The peaks observed belonging to phytochemicals in the FTIR spectrum showed that they were responsible for the stability of AgNPs. Phytochemicals surrounding VS-AgNPs caused a decrease in cytotoxicity. It was determined that the cytotoxic effects of VS-AgNPs on L929 cells started at a concentration of 50 μg/mL, while VSE started at a concentration of 1000 μg/mL indicating that the antimicrobial effect of VS-AgNPs was stronger than the plant extract VSE. The inhibition zone of VS-AgNPs against <em>S. aureus</em> strain was found to be 17.73 ± 1.19 mm, while the inhibition zone of VSE was found to be 9.66 ± 1.13 mm. The wound closure rate in VSE-applied cells was approximately 94 %, which was higher than the control group data. VS-AgNPs had no significant wound-healing effect. Finally, the photocatalytic effect of VS-AgNPs on the degradation of methylene blue dye was determined to be approximately 72 % after 3 h. These data indicate that VS-AgNPs may be a good alternative for environmental applications due to their antimicrobial effects and less cytotoxicity towards healthy cells.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103625"},"PeriodicalIF":3.4,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144190338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative stability in Atlantic salmon by-products: The role of reactive organs, processing methods, and antioxidants","authors":"Isabel Cuenca Flores ,&nbsp;Abul Hossain ,&nbsp;Yi Liu ,&nbsp;Deepika Dave","doi":"10.1016/j.bcab.2025.103623","DOIUrl":"10.1016/j.bcab.2025.103623","url":null,"abstract":"<div><div>The effect of the presence of reactive organs, antioxidants (rosemary extract), and processing methods (grinding and tumbling) on lipid oxidation in Atlantic salmon (<em>Salmo salar</em>) by-products was investigated. This study is the first to investigate the impact of individually separating all reactive organs from Atlantic salmon by-products and to assess their influence on lipid stability. Whole salmon heads, frames, and viscera were subjected to either tumbling or grinding to compare the effect of a high and low tissue disruption on their storage stability at 10 °C for 7 days. Additionally, salmon heads and viscera were stored either whole or after sorting highly pro-oxidant organs (reactive organs) to assess their role in lipid oxidation. During seven-day storage, peroxide and <em>p</em>-anisidine values and free fatty acid content were analyzed. Grinding reduced the oxidative stability of by-products, while sorting reactive organs increased the oxidative stability of heads and viscera lipids. Viscera lipids were the most susceptible to deterioration. Moreover, lipid oxidation during 90-day storage at −18 °C was evaluated, revealing that by-products without reactive organs remained stable irrespective of antioxidants addition or processing methods. Fatty acid analysis using gas chromatography-mass spectrometry (GC-MS) identified five primary polyunsaturated fatty acids, linoleic, alpha-linolenic, docosahexaenoic, eicosapentaenoic, and docosapentaenoic acids, which remained stable during storage. These findings highlight the importance of eliminating or inhibiting specific pro-oxidants and selecting appropriate processing methods to prevent lipid oxidation in Atlantic salmon by-products.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103623"},"PeriodicalIF":3.4,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of edible seaweed-based coating for postharvest preservation of mangoes","authors":"Manoely Virginia D.de B.S. Souza ,&nbsp;Athaline G. Diniz ,&nbsp;Valquíria Bruna Guimarães Silva ,&nbsp;Maria Daniela S. Buonafina-Paz ,&nbsp;José Ewerton F. dos Santos ,&nbsp;Wêndeo K. Costa ,&nbsp;Viviane L.X. de Souza Leão ,&nbsp;Fálba B.R. dos Anjos ,&nbsp;Wilson Barros Júnior ,&nbsp;Rejane P. Neves ,&nbsp;Patricia V. Tiago ,&nbsp;Severino Alves Júnior","doi":"10.1016/j.bcab.2025.103620","DOIUrl":"10.1016/j.bcab.2025.103620","url":null,"abstract":"<div><div>The post-harvest loss of approximately 28 % of mangoes in Brazil significantly contributes to global food waste, while millions face chronic hunger. It is essential to develop sustainable and low-cost solutions to reduce these losses, ensuring quality and food safety for end consumers. This study evaluated the efficacy of a sodium alginate biopolymer and aqueous extract of <em>Padina pavonica</em> seaweed-based active edible coating to preserve <em>Tommy Atkins</em> mangoes stored at 10 °C for 20 days. The <em>P. pavonica</em> extract showed high phenolic (736.17 mg GAE/g) and flavonoid (27.40 mg QE/g) content, with strong antioxidant activity (98.20 % DPPH reduction at 1000 μg mL⁻¹), and effectively inhibited bacterial and fungal growth, including <em>Staphylococcus aureus</em>, <em>Escherichia coli</em>, <em>Alternaria</em>, and <em>Colletotrichum</em>. Mangoes treated with the coating demonstrated improved quality and delayed ripening, evidenced by (i) clearer pulp, (ii) greater firmness suitable for commercialization, (iii) 3.27 % mass loss indicating delayed ripening, and (iv) absence of fungal diseases. These results highlight the coating's potential to extend shelf life and maintain mango quality during post-harvest storage. In addition, a technical-economic analysis revealed the coating's economic viability, offering cost savings exceeding 30 %, potentially reaching 50 %, compared to carnauba wax.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103620"},"PeriodicalIF":3.4,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144195664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical characterization of phytase and xylanase produced by Aspergillus japonicus using alternative carbon sources and in vitro hydrolysis of animal feed by the enzyme cocktail","authors":"Ana Lorena de Oliveira Simas ,&nbsp;Nathalia Nunes Glienke ,&nbsp;Quézia de Melo Santana ,&nbsp;Isabela Pavão Vargas ,&nbsp;Rodrigo Mattos Silva Galeano ,&nbsp;Charles Kiefer ,&nbsp;Karina Márcia Ribeiro de Souza Nascimento ,&nbsp;Douglas Chodi Masui ,&nbsp;Fabiana Fonseca Zanoelo ,&nbsp;Giovana Cristina Giannesi","doi":"10.1016/j.bcab.2025.103621","DOIUrl":"10.1016/j.bcab.2025.103621","url":null,"abstract":"<div><div>Grains are common sources of nutrients in diets for non-ruminant animals but contain antinutritional factors that limit digestibility. A widely used solution is the addition of bioactive enzymes to diets. Filamentous fungi synthesize numerous enzymes used in the animal feed industry. This study aimed to produce and characterize phytases (PhyAj) and xylanases (XynAj) from <em>Aspergillus japonicus</em> and assess their potential to improve the digestibility of animal feed as compared with commercial enzymes. Enzymes were produced by solid-state cultivation using alternative carbon sources. The highest production of both enzymes was achieved in 72 h, with the wheat bran and soybean bran substrate (1:1 w/w) yielding 10.23 ± 0.63 U/g for PhyAj and 31.83 ± 2.87 U/g for XynAj. PhyAj showed optimum activity at 55 °C and pH 4.5, whereas XynAj performed best at 55 °C and pH 5.5. PhyAj increased feed degradation by 90.15 % (A4), surpassing the effects of commercial enzymes. Similarly, XynAj enhanced feed degradation by 81.64 % (A6) and diet digestibility by 88.10 % (D7), surpassing the performance of commercial enzymes. <em>A. japonicus</em> enzyme cocktail significantly improved nutrient release, achieving a phosphorus release of 9.44 ± 0.44 mg/g from wheat bran (A7) and a reducing sugars release of 172.40 ± 2.71 mg/g from rice grits (A1) after 24h. These findings demonstrate that <em>A. japonicus</em> is a promising producer of commercially relevant enzymes, holding great potential to enhance nutrient bioavailability in animal diets.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103621"},"PeriodicalIF":3.4,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of anti-diabetic effects of ethanolic extract of Bougainvillea glabra","authors":"Chananda Saadprai ,&nbsp;Chartchai Chaichana ,&nbsp;Napachanok Swainson ,&nbsp;Watip Tangjittipokin ,&nbsp;Suttida Chukiatsiri","doi":"10.1016/j.bcab.2025.103617","DOIUrl":"10.1016/j.bcab.2025.103617","url":null,"abstract":"<div><div><em>Bougainvillea glabra</em> is a widely decorative plant. Previous research has shown that this plant contains bioactive compounds, including phenolics and flavonoids. In this study, bracts and flowers of <em>B. glabra</em> were extracted using ethanol. Folin-Ciocalteu's and aluminum chloride colorimetric assays were used to assess the total phenolic and flavonoid contents, respectively. Anti-diabetic activities were evaluated by α-amylase and α-glucosidase inhibition assays. Glucose uptake of HepG2 hepatocytes and L6 myoblasts was studied. The level of lipid accumulation in 3T3-L1 preadipocytes were assessed. The results revealed that the ethanolic extract of <em>B. glabra</em> exhibited higher inhibition of α-amylase and α-glucosidase activity than voglibose, a positive control. It also showed the higher of glucose uptake in HepG2 and L6. Moreover, ethanolic extract of <em>B. glabra</em> showed a better reduction of lipid accumulation in 3T3-L1 when compared to positive control. The results of the GC-MS analysis showed that the ethanolic extract of <em>B. glabra</em> had a high content of secondary metabolite with anti-diabetic potential. This study showed that the ethanolic extract of <em>B. glabra</em> had anti-diabetic effects, and it could develop for anti-diabetic treatment.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103617"},"PeriodicalIF":3.4,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144147551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}