Biocatalysis and agricultural biotechnology最新文献

{"title":"Glycolipopeptide biosurfactant produced by Klebsiella pneumoniae ssp. ozaenae BK34: Virtue in germination and growth of chickpea (Cicer arietinum L.) HC-1 crop","authors":"Mukesh Kumar ,&nbsp;Seema Sangwan ,&nbsp;Harpreet Kaur ,&nbsp;Pankaj Sharma ,&nbsp;Sushila Singh","doi":"10.1016/j.bcab.2024.103353","DOIUrl":"10.1016/j.bcab.2024.103353","url":null,"abstract":"<div><p>Modern agriculture consumes many persistent and non-biodegradable agrochemicals enhancing production and economy for farmers. Besides, chemical surfactants are used as adjuvant to augment the action of agrochemicals. A toxicity concern of these agrochemicals and adjuvants on life forms and environment cannot be ignored. Microbiologically produced, eco-friendly, biodegradable surfactants could be suggested as an alternative, however, compatibility with plants and beneficial microflora needs to be ascertained before applying them into agriculture. The current study included evaluation of biosurfactant BS34 produced by <em>Klebsiella pneumoniae</em> BK34 towards germination and growth of chickpea [<em>Cicer arietinum</em> (L.)] seeds. The biosurfactant BS34 was characterized as surfactin like glycolipopeptide based on Fourier transform infrared spectroscopy and Liquid chromatography-Mass spectrometry characterization. The biosurfactant was found non-phytotoxic during <em>in-vitro</em> germination of chickpea seeds as evident from very high germination index of 187.74 as opposed to the almost negligible germination index of 0.04 shown by highly phytotoxic chemical surfactant. In comparison to control, the biosurfactant seed treatment [1%(w/v)] in an <em>in-vivo</em> study on chickpea increased the plant height by 18%, nodule number by 11.13%, nodule fresh weight by 12.66% shoot weight by 11.20% and root weight by 34.61%. Contrarily, seed treatment by 1% (w/v) sodium dodecyl sulfate (SDS) inhibited the germination and growth of chickpea seeds again documenting superiority of biosurfactants over their chemical counterparts. Improved seed germination, growth and yield attributes of chickpea crop in presence of non-phytotoxic glycolipopeptide biosurfactant produced by <em>Klebsiella pneumoniae</em> ssp. <em>ozaenae</em> BK34 suggests its potential as an alternative to chemical surfactants used in agriculture.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tailor-made green composts with suppressive properties against tracheofusariosis of wild rocket (Diplotaxis tenuifolia): Useful option for sustainable circular horticulture","authors":"Catello Pane ,&nbsp;Andrea Pasqualini ,&nbsp;Riccardo Spaccini ,&nbsp;Angelica Galieni ,&nbsp;Alfonso Pentangelo ,&nbsp;Alessandro Esposito ,&nbsp;Roberto Altieri","doi":"10.1016/j.bcab.2024.103357","DOIUrl":"10.1016/j.bcab.2024.103357","url":null,"abstract":"<div><p>Intensive cultivation systems of wild rocket (<em>Diplotaxis tenuifolia</em>) are threatened by the outbreak of tracheofusariosis caused by the soil-borne fungal pathogen <em>Fusarium oxysporum f. sp. raphani</em>. The disease is difficult to eradicate by curative methods due to the endophytic progression of the pathogen. Suppressive composts can help to prevent this adversity by providing organic matter carrying chemical and biological components that can directly and/or indirectly interfere with the plant-pathogen system. In this study, a collection of seven promising composts from different selected green feedstocks were investigated for their suppressive properties towards wild rocket Fusarium wilting. <em>In planta</em> assays identified two composts, obtained from olive tree pruning + plant and tomato residues, and from olive waste + straw + wool residues, with the highest levels of suppressiveness, influenced by both application dose and plant development stage. These active composts showed overlapping ¹³C NMR profiles as <em>in vivo</em> suppressiveness, being positively correlated with the organic aliphatic alkyl, O-Aryl and carboxyl C fractions and subsequently with the hydrophobic index and alkyl ratio, and negatively with the lignin ratio. On the contrary, although biotic compost components showed antifungal effects, biological properties did not correlate with <em>in planta</em> wilting suppression. Consistently, reflectance spectroscopy in the Vis-NIR-SWIR highlighted the clustering of samples depending on their feedstock and chemical composition. The results allow the hypothesis of a plant-mediated suppression mechanism. The composting of crop residues improves the circularity of horticulture and makes it possible to produce, from selected matrices, tailored compost towards the sustainable cultivation of wild rocket.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142230246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of PGRs, polyamines and potassium to improve pomegranate flowering behaviour, fruit set and fruit quality","authors":"Pinky Raigond ,&nbsp;Nripendra V. Singh ,&nbsp;Amarja K. More ,&nbsp;Shilpa Parashuram ,&nbsp;Namrata A. Giri ,&nbsp;Chandrakant Awachare ,&nbsp;Roopa Sowjanya ,&nbsp;Prakash G. Patil ,&nbsp;K. Dhinesh Babu ,&nbsp;Rajiv A. Marathe","doi":"10.1016/j.bcab.2024.103355","DOIUrl":"10.1016/j.bcab.2024.103355","url":null,"abstract":"<div><p>Ever changing climate is affecting the flowering pattern in pomegranate worldwide. It is the need of the hour to find out some chemicals that can not only enhance hermaphrodite flowers and fruit set but also enhance fruit quality. The present investigation aimed to evaluate the effect of plant growth regulators (three), polyamines (two) and other chemicals (five) on photosynthesis, flowering ratio, fruit set and quality of pomegranate fruits. Pomegranate trees were treated at full leaf expansion stage. Potassium dihydrogen phosphate (5000 ppm), spermidine (1.5 ppm) and naphthalene acetic acid (NAA) (20 ppm) were the best treatments to significantly enhance hermaphrodite flowers, as the hermaphrodite to male flower ratio was 0.39, 0.37, and 0.35, respectively, compared to 0.22 in the control treatment. Except spermidine, NAA and paclobutrazol, all treatments increased the fruit setting significantly, with potassium dihydrogen phosphate (5000 ppm) being the most effective treatment with 66% fruit set compared to 43% in control trees. In mature fruits, compared to control, NAA enhanced total phenols, ascorbic acid and total antioxidants (3%, 15%, 7%, respectively) followed by ascorbic acid treatment (250 ppm); potassium dihydrogen phosphate (5000 ppm) and putrescine (44 ppm) enhanced the anthocyanin content and hence aril color significantly (11% and 10% respectively). Though the use of NAA in pomegranate production is commercial practice, two sprays of ascorbic acid can also be included to enhance the nutritional quality of fruits. Foliar application of potassium dihydrogen phosphate twice at flowering stage could be highly useful for quality pomegranate production due to the enhanced number of hermaphrodite flowers, fruit setting and fruit color. Overall, use of these chemicals can be beneficial to pomegranate farmers to get more remuneration from harvest.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142230244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungi species identified from polluted environment for chromium sequestration and solid state fermentation on tannery shaving waste","authors":"Birhanu Gizaw ,&nbsp;Tesfaye Alemu ,&nbsp;Girma Ebsa ,&nbsp;Zerihun Tsegaye","doi":"10.1016/j.bcab.2024.103352","DOIUrl":"10.1016/j.bcab.2024.103352","url":null,"abstract":"<div><p>Accumulation of hexavalent chromium in the environment affects our ecosystem. Physico-chemical treatment did not solve the problem, due to high requirement of operational costs &amp; energy, insolubility of Cr (VI) at any pH &amp; sludge accumulation. The study aim is screening and evaluating fungi for chromium biosorption for mycoremediation. Soil and effluent sample were collected. PDA media was used for fungi purification. Fungal chromium tolerance evaluation and optimization was conducted at pH (4, 7, 10), T⁰ (25, 27.5, 35 °C), Cr concentration (5000–25000 ppm) using Full Factorial experimental design. Spore count, biomass production, Cr tolerance index, mycelia growth were evaluated. Flame Absorptive Spectrophotometer was used for quantification of fungal chromium removal. Fungi species were identified by MALDI-TOF. Fungi growth &amp; degradation of tannery shaving waste was evaluated. The result revealed 6 potential fungi, <em>T. longibrachiatum, T, koningii, A. niger, G</em>. <em>candidum, P. rubens, T. orientale</em> were identified. Optimium mean biomass &amp; spore count were 0.514 ± 0.051&amp; 1.39 ± 0.038 at pH4 and 27.5 °C respectively. MTL test indicate that <em>Trichoderma</em> species, <em>A. niger &amp; P. rubens</em> survive up to 10,000 ppm, 15, 000 ppm &amp; 20,000 ppm respectively. FAAS analysis of fungi chromium removal from aqueous media result indicate that <em>A. niger</em> (99%). <em>T. longibrachiatum</em> (85%) and <em>P. ruben</em> (84.6%). These 6 fungi are efficient in tannery shaving waste degradation but <em>A. niger</em> was superior in spore count 15.1 Log/mL and 7 g substrate weight loss. In conclusion three fungi have high chromium tolerance and removal capacity and shaving waste degradation promising for mycoremediation.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic insights on the antibiofilm potential of snake venom peptides: An in silico based molecular docking approach","authors":"Moupriya Nag","doi":"10.1016/j.bcab.2024.103351","DOIUrl":"10.1016/j.bcab.2024.103351","url":null,"abstract":"<div><p>The threat of antibiotic resistance to the public's health is exacerbated by the emergence of multidrug resistant bacterial (MDR) strains and their biofilm. Antibiotics are unable to penetrate the thick biofilm matrix causing the indwelling bacterial cells to survive resulting into the origin of antimicrobial resistance (AMR). Thus, biofilms are responsible for causing many chronic infections such as dental plaque formation, cystic fibrosis, urinary tract infections (UTI), otitis media etc. Hence, there is an urgent need for alternate therapeutic strategies for preventing bacterial biofilm formation and fighting AMR. In this regard antimicrobial peptides (AMPs) that have broad-spectrum antibacterial action with significant specificity and low toxicity, are a great choice to combat AMR. Peptides derived from snake venom such as cathelicidins reflects a spectrum of biological actions including its antimicrobial potential. The present study focuses on screening and identifying AMPs having anti-biofilm properties. This includes natural cathelicidins found in snake venom as a potential antibacterial agent to combat AMR. Two biofilm forming proteins were identified viz. icaC protein of <em>Staphylococcus aureus</em> &amp; bdlA protein of <em>Pseudomonus aureginosa</em>. AMPs were screened from DRAMP Database and snake venom cathelicidins were selected for further studies. The binding interaction of cathelicidins with the icaC and bdlA protein were investigated using molecular docking studies. Based on the interacting amino acid residues, antigenic determinants, peptide binding site and docking scores a performance scale was built to classify relevant snake venom cathelicidins according to their importance for further <em>in vivo</em> and <em>in vitro</em> antibiofilm investigations.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the potential of aqueous enzymatic extraction of safflower (Carthamus tinctorius L.) seed oil: Process optimization and oil characterization","authors":"Chaymae Benkirane ,&nbsp;Abdessamad Ben Moumen ,&nbsp;Aymane Allay ,&nbsp;Youssef Rbah ,&nbsp;Mariya Barkaoui ,&nbsp;Hana Serghini Caid ,&nbsp;Ahmed Elamrani ,&nbsp;Farid Mansouri","doi":"10.1016/j.bcab.2024.103354","DOIUrl":"10.1016/j.bcab.2024.103354","url":null,"abstract":"<div><p>Safflower seed oil was extracted by aqueous enzymatic extraction (AEE) with a combination of three enzymes: hemicellulase, pectinase, and protease. A Response Surface Methodology (RSM) was performed to optimize the operating conditions: Temperature, pH, and incubation time. Analysis of variance allowed studying the variables' linear, quadratic, and interaction effects. The optimized extraction conditions resulted in 85% oil recovery referring to the conventional Soxhlet extraction (SE). Subsequently, a comparative analysis was conducted to evaluate the quality and composition of oils obtained by SE and AEE. The results revealed that the two oils had similar values of peroxide index, conjugated dienes and trienes, and fatty acid composition. AEE-oil had a lighter yellow color (L* = 31.8 Vs. 24.7) and was richer in polyphenols (147 Vs. 101 mg GAE/Kg of oil), but it was more acidic (2.75% Vs. 1.56% linoleic acid) and less stable (2.86h Vs. 5.66h induction time) with less tocopherol (302 Vs. 409 mg/kg oil) and carotenoid content (0.42 Vs. 0.57 mg/kg oil) than SE-oil. Our findings demonstrated that RSM-based optimization is an efficient and time-saving approach, which significantly improved oil extraction. Finally, we suggested several solutions to further improve the quality and quantity of the obtained AEE-safflower seed oil, paving the path for other future research possibilities.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142171824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing fruit quality and yield in tomato through cyanobacterium mediated nutri-fertigation","authors":"Ravi Mourthy Nivedha ,&nbsp;Akanksha Bhardwaj ,&nbsp;Radha Prasanna ,&nbsp;Nallapareddy Bavana ,&nbsp;Venkatesh Kokila ,&nbsp;Sekar Nishanth ,&nbsp;Shalini Gaur Rudra ,&nbsp;Awani Kumar Singh ,&nbsp;Kadapa Sreenivasa Reddy ,&nbsp;Yashbir Singh Shivay","doi":"10.1016/j.bcab.2024.103344","DOIUrl":"10.1016/j.bcab.2024.103344","url":null,"abstract":"<div><p>Protected cultivation of high-value crops such as tomato, with several health benefits, is gaining global significance. This study explored the influence of aqueous formulations of cyanobacteria-<em>Anabaena laxa</em> (C11), <em>Nostoc carneum</em> (BF2), and <em>Anabaena doliolum</em> (BF4), applied as soil drench at pre-flowering and fruiting stages, on the dynamics of soil and plant attributes important for growth, productivity, and quality of fruits in an indeterminate tomato variety (<em>NS4266</em>) under protected cultivation. Drenching with the BF2 formulation resulted in a significant fold-increase in soil dehydrogenase activity and an increase of 40–45% in soil nitrogen availability; positively impacted glutamine synthetase activity, fruit weight, lycopene content, skin elasticity, in comparison to control. C11 drenching led to a 17% elevation in soil microbial biomass carbon, besides bringing about a 24 and 54% enhancement in available phosphorus and iron content. Additionally, it also enhanced chlorophyll <em>a, b</em> and total pigments by 54–65% over control. Principal Component Analysis (PCA) and Multivariate analysis showcased the distinct effects of cyanobacterial formulations on crop growth and soil fertility, as well as the significant and positive correlations among quality, plant, soil, and yield attributes contributed through cyanobacterial drenching. This investigation highlighted cyanobacterial drench as a promising organic option to augment soil nutrient availability, bolster overall productivity, and enhance fruit quality in tomato grown under protected cultivation.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alleviation of salt stress on Zea mays L. plant by PGPR isolates as an effective sustainable strategy","authors":"Fawkia A. El-Amriti ,&nbsp;Salama A. Ouf ,&nbsp;Mohammed Abu-Elghait ,&nbsp;Said E. Desouky ,&nbsp;Mahmoud S.M. Mohamed","doi":"10.1016/j.bcab.2024.103346","DOIUrl":"10.1016/j.bcab.2024.103346","url":null,"abstract":"<div><p>Among the major constraints for the production of economic crops, such as maize, is salinity. Plenty of research has suggested the use of various strains of plant growth-promoting rhizobacteria (PGPR) as a sustainable approach to improve plant tolerance to salinity. Nonetheless, the exploration of the rhizosphere in Egyptian agricultural lands with salt affected soil remains incomplete. In this research, two newly isolated local bacterial species were finally selected and identified as <em>Alcaligenes</em> sp. strains. E1 (BS-45) and E2 (BS-57). IAA production by both isolates was confirmed by TLC. Several bioactive metabolites were identified in <em>Alcaligenes</em> sp. E1 and E2 by gas chromatography-mass spectroscopy (GC-MS). These include 9,12,15-octadecatrienoic acid, 2,3-bis [(trimethylsilyl)oxy] propyl ester, (z,z,z), 2 (1H)-Naphthalenone, octahydro-1-methyl-1-(2-propenyl)-, (1à,4aá,8aà), dodecane and isopulegol which have a defensive role against biotic and abiotic stress of the plant. Interestingly, the selected bacteria <em>Alcaligenes</em> sp. E1 and E2 significantly improve the overall maize growth attributes, antioxidant scavenging activities and photosynthetic pigments in maize plants grown under salt stress (150 mM NaCl) compared to the control plants as analyzed by greenhouse experiment. Therefore, this study recommends the <em>Alcaligenes</em> sp. strains (E1 and E2) to alleviate the negative impact of salinity on the growth and development of maize seedlings.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142239865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of novel marine bacteria isolated from deep Atlantic Ocean in Morocco as a source of natural bioactive compounds to control infections in aquaculture","authors":"Asmaa Chbel ,&nbsp;Manuel Rey-Méndez ,&nbsp;Juan L. Barja ,&nbsp;Aurelio Serrano-Delgado ,&nbsp;Abdelaziz Soukri ,&nbsp;Bouchra El Khalfi","doi":"10.1016/j.bcab.2024.103350","DOIUrl":"10.1016/j.bcab.2024.103350","url":null,"abstract":"<div><p>With the rapid expansion of aquaculture production, there has been a concomitant increase in fish diseases, generally combatted by the utilization of chemicals and antibiotics. However, their overuse leads to the emergence of drug-resistant microorganisms and the appearance of oxidative stress. Consequently, antibiotics contribute to a reduction in fish immune defense. In this work, eight marine bacteria were characterized using physiological, biochemical and microbiological tests, and were evaluated for their safety by studying their haemolytic and gelatinase activities. Then, we evaluated their ability to produce active biomolecules and tested their <em>in vitro</em> antibacterial activities against five bacterial pathogens that are highly prevalent in aquaculture. Moreover, we evaluated their antioxidant potential using DPPH, ABTS and ferric reducing power tests. The obtained results revealed that cell-free supernatants were effective, they exhibited good antioxidant and antibacterial activities. Their biomolecules showed good stability under different temperatures ranging from 60 to 121 °C and were found to be chemically composed of peptides, exopolysaccharides and polyphenols. Therefore, the secreted bioactive substances are of great biotechnological interest and constitute a promising eco-friendly alternative to antibiotics and chemical agents in the context of sustainable aquaculture.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Entrapment of glucose oxidase from Aspergillus niger ISL-09 in poly(acrylamide-co-acrylic acid) hydrogels for improved stability and catalytic efficiency towards industrial applications","authors":"Rukhma ,&nbsp;Sikander Ali ,&nbsp;Muhammad Jahangeer ,&nbsp;Muti Ur Rehman ,&nbsp;Iqra Liyaqat ,&nbsp;Sarmad Ahmad Qamar","doi":"10.1016/j.bcab.2024.103349","DOIUrl":"10.1016/j.bcab.2024.103349","url":null,"abstract":"<div><p>The present study highlights the true potential of <em>Aspergillus niger</em> ISL-09 to produce glucose oxidase (GOx) and focuses on improving the catalytic properties of GOx by entrapping it in poly acrylamide-co-acrylic acid (AAm-co-AAc) hydrogels, fabricated by free-radical polymerization method. For this purpose, the optimum conditions for GOx activity were found to be: 15 g soybean meal (SBM) substrate, 20 mL moisture content, 96 h incubation period, and 1.5 mL of fungal spore suspension. The purified extracellular GOx was observed to have 32.4% yield, with 0.02 U/mg specific activity and approximately 80 kDa subunit molecular weight. The GOx was most active at pH 5.5 and 40 °C. The immobilization of GOx in poly (AAm-co-AAc) hydrogels led towards improved stability and catalytic efficiency, resulting in a 21.7% increase in activity compared to free enzyme. The study also examined the potential of GOx in the pharmaceutical and textile industries as Ca-gluconate producer and bleaching agent, respectively. The study concludes that <em>A. niger</em> ISL-09 is a promising source for GOx production under optimal conditions. Furthermore, the immobilization of GOx in poly (AAm-co-AAc) hydrogels can significantly improve its catalytic properties, making it suitable for different industrial applications. However, further scaling up is required for the better implementation in industry.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142158290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}