Biocatalysis and agricultural biotechnology最新文献

{"title":"Analysis of nucleotide diversity among alleles of intronless bacterial blight resistant genes Xa27 and Xa23 in rice","authors":"Prajna Priyadarshini Das ,&nbsp;Kailash Pati Bhandari ,&nbsp;Komal Bhati ,&nbsp;C. Gireesh ,&nbsp;Gouri Sankar Laha ,&nbsp;Raman Meenakshi Sundaram ,&nbsp;Irfan Ahmad Ghazi","doi":"10.1016/j.bcab.2024.103477","DOIUrl":"10.1016/j.bcab.2024.103477","url":null,"abstract":"<div><div>Identifying novel effective alleles from different gene pools can provide a stress-free response in any crop plant by allele mining approach. Nucleotide diversity analysis through allele mining helps to study diverse function of genes and their role in disease resistance enhancement. The executor R genes; <em>Xa27</em> and <em>Xa23</em> provide resistance against several strains of the rice bacterial blight pathogen, <em>Xanthomonas oryzae</em> pv. <em>Oryzae</em> (<em>Xoo</em>). A total 114 wild relatives of <em>Oryza</em> species were examined to assess nucleotide diversities among the natural alleles of the two genes. Among these, 21 accessions were identified as homologous to <em>Xa27</em>, and 5 accessions were homologous to <em>Xa23</em>. Pathogenicity test was conducted to assess the resistance efficiency of the gathered wild accessions using disease scoring assays for phenotyping. Various parameters for the nucleotide diversity study, including polymorphic sites, InDels, number of haplotypes, single nucleotide polymorphisms, as well as Tajima's D and Fu and Li's D tests, were analyzed for the identified alleles in both the promoter, Effector Binding Element (EBE), and coding regions of these two genes. <em>Xa27</em> alleles from the rice accessions such as CG86, CG105, CG180, and CG02 exhibit higher numbers of substitutions compared to other identified alleles. CG12 accession showed numerous nucleotide substitutions, indicating a possible novel allele unrelated to <em>Xa23</em> alleles. Molecular mining of <em>Xa27</em> and <em>Xa23</em> in wild rice relatives will help to identify the major novel allele for the breeding program to enhance crop improvement.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103477"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Valorization of palm petiole waste as natural biocoagulants: Optimizing coagulation-flocculation for sustainable wastewater treatment and advancing circular economy in agriculture","authors":"Ali Zourif,&nbsp;Salma Kouniba,&nbsp;Mohamed El Guendouzi","doi":"10.1016/j.bcab.2024.103473","DOIUrl":"10.1016/j.bcab.2024.103473","url":null,"abstract":"<div><div>This study investigates the potential of <em>palm petiole waste</em> (PPW) and its extracted lignin as natural biocoagulants for removing methylene blue, crystal violet dyes, and turbidity from wastewater. Comprehensive characterization using X-ray diffraction, fourier-transform infrared spectroscopy, scanning electron microscopy coupling with energy-dispersive X-ray spectroscopy, X-ray fluorescence, and elemental analysis revealed the distinct physicochemical properties of PPW and lignin. A Box-Behnken experimental design optimized coagulation conditions, achieving 98.26 % pollutant removal with PPW and 90.17 % with lignin. Crucially, PPW's superior performance stems from a synergy of adsorption, charge neutralization by metal salts, and bridging effects, while lignin relies primarily on adsorption and bridging. This challenges the notion that lignin alone dictates coagulation efficacy in lignocellulosic wastes. The study also explored the economic and environmental implications of using PPW as a bio coagulants-flocculant, demonstrating significant reductions in carbon footprint, energy consumption, and water use compared to conventional chemical coagulants.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103473"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Capsicum chinense cell cultures: A biotechnological platform for the sustainable production of bioactive metabolites for the cosmetics market","authors":"Amir Akhgari ,&nbsp;Maria Sulli ,&nbsp;Anna Ramata-Stunda ,&nbsp;Suvi T. Häkkinen ,&nbsp;Liisa Nohynek ,&nbsp;Aleksander Salwinski ,&nbsp;Olivia Costantina Demurtas ,&nbsp;Mārtiņš Borodušķis ,&nbsp;Maria Pajumo ,&nbsp;Richard M. Twyman ,&nbsp;Kirsi-Marja Oksman-Caldentey ,&nbsp;Gianfranco Diretto ,&nbsp;Heiko Rischer","doi":"10.1016/j.bcab.2024.103478","DOIUrl":"10.1016/j.bcab.2024.103478","url":null,"abstract":"<div><div>Capsicum species produce a broad range of bioactive metabolites with properties relevant to the cosmetics industry. However, the direct sourcing from Capsicum plants is hindered by the low and variable yields, which depend on genotype and environmental conditions, and by the pungent components of chili plants that act as irritants. We therefore set out to derive callus and cell suspension cultures from the non-pungent variety <em>C. chinense</em> cv. Trinidad Pimento as a sustainable and consistent source. Cultures were grown at the 2-L scale in darkness (D) or with a photoperiodic light source (P) and we prepared hydroethanolic and ethyl acetate extracts for the analysis of metabolic profiles and activities. Comprehensive untargeted metabolomic analysis revealed different clusters of metabolites under D and P conditions, although phenolamides and fatty acids were the most abundant compounds in both cases. Targeted analysis indicated the presence of capsiate, dihydrocapsiate and nordihydrocapsiate as key bioactive products in the hydroethanolic extracts and 18 different carotenoids in the ethyl acetate extracts. Bioactivity assays applied to the hydroethanolic extracts indicated significant antioxidant activity and the ability to inhibit the growth of methicillin resistant Staphylococcus aureus. The extracts were neither cytotoxic nor phototoxic toward BALB/c 3T3 and HaCaT cell lines at concentrations low enough to exert antimicrobial effects. Our work shows that <em>C. chinense</em> cv. Trinidad Pimento cell cultures can produce specific metabolites in response to different cultivation conditions, which will allow their exploitation as a sustainable source of safe and efficacious ingredients for the cosmetics industry.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103478"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking plant chemical diversity: Exploring epigenetic influences on secondary metabolite production","authors":"Shifali Gupta ,&nbsp;Sanjeev Kumar Sahu ,&nbsp;Paranjeet Kaur ,&nbsp;Thakur Gurjeet Singh ,&nbsp;Sovia RJ. Singh","doi":"10.1016/j.bcab.2024.103445","DOIUrl":"10.1016/j.bcab.2024.103445","url":null,"abstract":"<div><div>The term epigenetics originates from the complicated interactions between the genome and surroundings that are included in advancement and differentiation in the life forms. In today's era genetic changes do not restrict to the modifications within the DNA sequence instead how it can be read or sensed by the body itself. As a substitute, epigenetic adjustments or markers, such as DNA methylation and histone modification, modify DNA accessibility and chromatin shape, subsequently controlling styles of quality expression. These methods are important for the day-to-day betterment and differentiation of genetic cells within the grown-up living beings. They may be changed by means of outside impacts and, as such, can contribute to or be an effect of natural alterations inside the phenotype or disease phenotype. Critically, epigenetic programming has an essential role in the direction of reprogramming the pluripotent genes in response to surroundings, which end up inactivated during differentiation. Currently, we review about epigenetic modification in plant secondary metabolites biosynthesis, mechanisms involved, examples of epigenetic modifications in classes of plant secondary metabolites such as terpenoids, phenolic compounds, alkaloids and sulphur containing compounds, epigenetic modification editing and engineering tools and its future perspectives.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"62 ","pages":"Article 103445"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review of nanomaterials synthesis: Physical, chemical, and biological approaches and emerging challenges","authors":"Pooja V. Nagime ,&nbsp;Vaishali S. Chandak","doi":"10.1016/j.bcab.2024.103458","DOIUrl":"10.1016/j.bcab.2024.103458","url":null,"abstract":"<div><div>Physical, chemical, and biological approaches are used in the manufacturing of nanomaterial; each has advantages and disadvantages of its own. Physical techniques like ball milling and laser ablation offer a regulated size distribution and great purity, but they cost a lot of money and energy. Particle dimensions and shape can be precisely controlled using chemical techniques, such as sol-gel as well as chemical vapor deposition. However, these processes frequently involve toxic chemicals and result in dangerous byproducts. Viral, plant, and microbe synthesis is achieved through biological processes. Enzymatic reduction &amp; capping are harnessed by microbial synthesis, although it can be sluggish and necessitates careful management of growing conditions. Plant-based synthesis, which offers a more environmentally friendly option but with variable particle size, employs phytochemicals for stabilization and reduction. This review aims to direct future study and implementation in nanotechnology by thoroughly comparing various approaches and highlighting their operational processes, environmental consequences, and possibilities for large-scale manufacturing.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"62 ","pages":"Article 103458"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143178979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel phycoerythrin-derived peptide from Colaconema formosanum: Synthesis, in vitro, and in silico study on angiotensin-converting enzyme (ACE) inhibitory activity","authors":"Seto Windarto ,&nbsp;Meng-Chou Lee ,&nbsp;Happy Nursyam ,&nbsp;Jue-Liang Hsu","doi":"10.1016/j.bcab.2024.103452","DOIUrl":"10.1016/j.bcab.2024.103452","url":null,"abstract":"<div><div><em>Colaconema formosanum</em>, the red algae used in this study, has never been documented for its bioactivities as an angiotensin-converting enzyme inhibitor (ACEI). This study aims to discover ACEI derived from <em>C. formosanum</em> with a mixture of enzymes and orthogonal bioassay-guided fractionation through <em>in vitro</em> and <em>in silico</em> approaches. LRDGEIILR (LR-9) was isolated from the protein hydrolysate of <em>C. formosanum</em> digested by a combination of proteases (pepsin and thermolysin). It is derived from phycoerythrin, a protein abundant in most red algae. The LR-9 has an IC₅₀ value of 94.28 ± 1.84 μM. The kinetics of inhibition revealed LR-9 to be a competitive inhibitor. The results of kinetic studies were rationalized further using molecular docking, which showed that LR-9 interacts preferentially with critical residues in the ACE active site. Based on the toxicity prediction, LR-9 was non-toxic. This study suggests that ACEI peptides extracted from <em>C. formosanum</em> could be an alternative source for developing hypertension-treating functional foods.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"62 ","pages":"Article 103452"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical characterization of a partially purified thermostable alkaline protease of Bacillus cereus isolated from a slaughterhouse effluent","authors":"Kehinde O. Awojobi ,&nbsp;Juliet Oluwayemisi Richard-Omole","doi":"10.1016/j.bcab.2024.103439","DOIUrl":"10.1016/j.bcab.2024.103439","url":null,"abstract":"<div><div>Proteases are applicable in the leather industry but with high production costs. There is a need to produce low-cost proteases with sufficient purity for animal skin dehairing. Hence, this study extracted, partially purified, biochemically characterized and applied alkaline protease synthesized by <em>Bacillus cereus</em> to remove animal hair. A crude alkaline protease optimally synthesized by <em>Bacillus cereus</em> in skimmed milk-submerged fermentation medium was purified by glycerol precipitation and CM-Sephadex C-50. The purified enzyme was classified and the kinetic parameters were determined. The purified protease had 723.1 U/mg specific activity at 1.5 purification fold. Purified protease was stable over widely-ranging pH (3–11) and temperature (20–80 °C), with the highest activity at pH 7.0 and 50 °C. The enzyme was specific for casein, with utmost stability at 60 °C, and maintained most of its activity for 60 min. Na⁺, K⁺, Fe³⁺, and NH₄⁺ inhibited the activity, while Ca²⁺ and Al³⁺ increased the activity. The K_m and V_max were correspondingly 4.82 mg/ml and 46.62 U/ml/min. The purified enzyme has the prospect of removing animal hair in the leather industry.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"62 ","pages":"Article 103439"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Metarhizium anisopliae on the histochemistry and enzymatic activities of Helicoverpa armigera (Hübner, 1809)","authors":"Yamini R ,&nbsp;Shanmugam P.S ,&nbsp;Murugan M ,&nbsp;Geetha N ,&nbsp;Meenakshi P ,&nbsp;Srinivasan T ,&nbsp;Premalatha K ,&nbsp;Kandan A ,&nbsp;Sathiyaraj G ,&nbsp;Vivekanandhan P","doi":"10.1016/j.bcab.2024.103443","DOIUrl":"10.1016/j.bcab.2024.103443","url":null,"abstract":"<div><div><em>Metarhizium anisopliae</em>, is widely utilized as a biocontrol agent in agriculture due to its diverse mechanisms of action and adaptability to various field conditions. However, the immune responses of the target insects significantly influence <em>M. anisopliae</em> virulence against insect pests. Among these pests, the tomato fruit borer, <em>Helicoverpa armigera</em>, stands out as a polyphagous and economically significant species for its resistance to numerous insecticides. Understanding the interaction between <em>M. anisopliae</em> and <em>H. armigera</em> immune defense is therefore critical for developing effective biocontrol strategies. In this study, five isolates of <em>M. anisopliae</em> were evaluated against the second and fourth instars of <em>H. armigera</em> using leaf dip-bioassay under controlled laboratory conditions. The isolate ICAR SBI Ma 08 was the most effective against both instars, with lowest LC₅₀ (2.56 × 10⁶ &amp; 3.56 × 10⁸) and LT₅₀ values (4.36 and 6.36 days). The order of efficacy of other strains was ICAR SBI Ma 01 &gt; ICAR SBI Ma 69 &gt; ICAR SBI Ma 04 &gt; ICAR SBI Ma 172. This isolate effectively evaded the immune response of <em>H. armigera</em> by inhibiting the activities of detoxifying enzymes such as esterase, mixed-function oxidase, acetylcholinesterase, and polyphenol oxidase. Conversely, following infection with this potent strain, larvae exhibited increased activities of glutathione S-transferase, catalase, aryl-acylamidase, and peroxidase. An increase in spore concentration had a positive impact on <em>M. anisopliae</em> pathogenicity. Histopathological studies showed that ICAR SBI Ma 08 successfully breached the first epidermal layer, with mycelia reaching the promeristem within 24 h post-infection. <em>M. anisopliae</em> ICAR SBI Ma 08 exhibited the highest efficacy against the second instar with a reduced LT₅₀, and its modulating enzymatic activity suggested that this isolate could be effectively deployed against the first and second instars of <em>H. armigera</em>. Developing an appropriate formulation and ascertaining its efficacy at the field level will confirm ICAR SBI Ma08's potential as a mycoinsecticide.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"62 ","pages":"Article 103443"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gac fruit (Momordica cochinchinensis Spreng): From nutritional value to food processing technology","authors":"Siti Alyani Mat ,&nbsp;Hesham Ali El Enshasy ,&nbsp;Nadirah Abd Rahim ,&nbsp;Xin Nih Chong ,&nbsp;Sajetra Devi Vadiveloo ,&nbsp;Harisun Ya'akob ,&nbsp;Daniel Joe Dailin ,&nbsp;Dennis Sing Teong Chew ,&nbsp;Nor Hasmaliana Abdul Manas","doi":"10.1016/j.bcab.2024.103444","DOIUrl":"10.1016/j.bcab.2024.103444","url":null,"abstract":"<div><div>Gac (<em>Momordica cochinchinensis</em> Spreng.) fruit, which originates from Southeast Asia, is widely recognized for its rich nutritional profile and its traditional medicinal applications. The fruit's commercialization in functional food products is driven by its potent antioxidant, antibacterial, anti-inflammatory, anticancer, neurotropic, and immunomodulatory properties. Additionally, Gac fruit is not widely consumed commercially as food, making it less competitive in the food market. However, processing can compromise these benefits due to the degradation of active compounds under harsh conditions. This review explores the nutrient composition and functional properties of Gac fruit, evaluates common processing techniques, and highlights strategies for nutrient preservation during processing. Emphasizing the importance of continued research, innovative processing, and sustainable practices, the article underscores the potential of Gac fruit to significantly impact global health and wellness. Effective preservation strategies are crucial not only for enhancing consumer health but also for bolstering the economic viability of Gac fruit in the global market.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"62 ","pages":"Article 103444"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizations of ash derived from the crops’ waste biomass for soil improvement and assisted phytoremediation","authors":"Abdulmannan Rouhani ,&nbsp;Valentina Pidlisnyuk ,&nbsp;Karim Suhail Al Souki","doi":"10.1016/j.bcab.2024.103456","DOIUrl":"10.1016/j.bcab.2024.103456","url":null,"abstract":"<div><div>The biomass ash can play a crucial role in agriculture by acting as a valuable fertilizer that fosters nutrient cycles and contributes to the conservation of nutrient's resources. The paper begins by discussion of the chemical properties of ashes derived from the herbaceous and agricultural crops. It then evaluates the effect of biomass ash on the soil properties and plant growth in the field and greenhouse conditions. Finally, the paper evaluates the impact of ash to phytoremediation practices, when nutrients presented in biomass ash were returned to the natural cycles to secure sustainable biomass utilization. Beside nutrients, some potentially toxic elements were presented in biomass ash which can threaten the environment. Rice husk ash followed by bagasse and wheat straw ashes were the most studied materials which showed positive effects on agricultural soil and crops. Furthermore, a positive impact of rice husk ash on the phytoremediation efficiency of <em>Ricinus communis</em> and <em>Vetiver Grass</em> were reported. The results of the current studies show a great potential of biomass ash when applying for the agricultural and environmental remediation actions thorough the careful assessment which ensures the circularity in the revitalization.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"62 ","pages":"Article 103456"},"PeriodicalIF":3.4,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}