Phytochemical profiling and evaluation of antimicrobial, cytotoxic, wound-healing, and photocatalytic properties of green-synthesized silver nanoparticles using Verbascum splendidum

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology Pub Date : 2025-05-29 DOI:10.1016/j.bcab.2025.103625

Ömer Hazman , Ibrahim Erol , Gofur Khamidov

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Abstract

Recent trends in green chemistry highlight the increasing importance of environmentally friendly nanoparticle synthesis. Recent studies highlight the environmental advantages and industrial potential of biogenic synthesis pathways. Silver nanoparticle (AgNPs) production was carried out using V. splendidum aqueous extract (VSE) by green synthesis, which is an environmentally friendly, cheap, and easily applicable method. In the content of VSE determined by the LC-MS/MS system, it was determined that the species responsible for the stabilization of AgNPs were phenolic acid and flavonoids. The formation of AgNPs was understood from the surface plasma resonance peak at 402 nm and the peaks showing the face-centered cubic silver crystalline structure in X-ray powder diffraction analysis. The peaks observed belonging to phytochemicals in the FTIR spectrum showed that they were responsible for the stability of AgNPs. Phytochemicals surrounding VS-AgNPs caused a decrease in cytotoxicity. It was determined that the cytotoxic effects of VS-AgNPs on L929 cells started at a concentration of 50 μg/mL, while VSE started at a concentration of 1000 μg/mL indicating that the antimicrobial effect of VS-AgNPs was stronger than the plant extract VSE. The inhibition zone of VS-AgNPs against S. aureus strain was found to be 17.73 ± 1.19 mm, while the inhibition zone of VSE was found to be 9.66 ± 1.13 mm. The wound closure rate in VSE-applied cells was approximately 94 %, which was higher than the control group data. VS-AgNPs had no significant wound-healing effect. Finally, the photocatalytic effect of VS-AgNPs on the degradation of methylene blue dye was determined to be approximately 72 % after 3 h. These data indicate that VS-AgNPs may be a good alternative for environmental applications due to their antimicrobial effects and less cytotoxicity towards healthy cells.

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绿色合成银纳米粒子的抗菌、细胞毒性、伤口愈合和光催化性能的植物化学分析和评价

绿色化学的最新趋势突出了环境友好型纳米颗粒合成的重要性。最近的研究强调了生物合成途径的环境优势和工业潜力。采用绿色合成的方法，利用V. spldidum水提物（VSE）生产纳米银粒子（AgNPs），这是一种环境友好、成本低廉、易于应用的方法。在LC-MS/MS系统测定的VSE含量中，确定对AgNPs起稳定作用的物质为酚酸和黄酮类化合物。AgNPs的形成可以从402 nm处的表面等离子体共振峰和x射线粉末衍射分析中显示的面心立方银晶体结构中理解。在FTIR光谱中观察到属于植物化学物质的峰，表明它们对AgNPs的稳定性负责。围绕VS-AgNPs的植物化学物质导致细胞毒性降低。实验结果表明，VS-AgNPs对L929细胞的细胞毒作用始于50 μg/mL，而VSE则始于1000 μg/mL，表明VS-AgNPs的抑菌作用强于植物提取物VSE。VS-AgNPs对金黄色葡萄球菌的抑制带为17.73±1.19 mm，对VSE的抑制带为9.66±1.13 mm。应用vse的细胞创面愈合率约为94%，高于对照组数据。VS-AgNPs无明显创面愈合作用。最后，在3小时后，测定了VS-AgNPs对亚甲基蓝染料降解的光催化作用约为72%。这些数据表明，由于其抗菌作用和对健康细胞的细胞毒性较小，VS-AgNPs可能是环境应用的良好替代品。

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来源期刊

Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

7.70

自引率

2.50%

发文量

308

审稿时长

48 days

期刊介绍： Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.