三角梅乙醇提取物抗糖尿病作用的评价

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology Pub Date : 2025-05-24 DOI:10.1016/j.bcab.2025.103617

Chananda Saadprai , Chartchai Chaichana , Napachanok Swainson , Watip Tangjittipokin , Suttida Chukiatsiri

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引用次数: 0

摘要

九重葛是一种广泛的装饰植物。先前的研究表明，这种植物含有生物活性化合物，包括酚类物质和类黄酮。本研究采用乙醇萃取法提取了光草的苞片和花。采用Folin-Ciocalteu比色法和氯化铝比色法分别测定总酚和类黄酮的含量。通过α-淀粉酶和α-葡萄糖苷酶抑制试验评价其抗糖尿病活性。研究HepG2肝细胞和L6成肌细胞的葡萄糖摄取。评估3T3-L1前脂肪细胞的脂质积累水平。结果表明，与阳性对照voglibose相比，光草乙醇提取物对α-淀粉酶和α-葡萄糖苷酶活性的抑制作用更高。HepG2和L6对葡萄糖的摄取也较高。此外，与阳性对照相比，光草醇提物能更好地减少3T3-L1的脂质积累。气相色谱-质谱分析结果表明，光草醇提物具有较高的次生代谢物含量，具有抗糖尿病作用。本研究表明，光刺草醇提物具有抗糖尿病作用，具有开发抗糖尿病药物的潜力。

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Evaluation of anti-diabetic effects of ethanolic extract of Bougainvillea glabra

Bougainvillea glabra is a widely decorative plant. Previous research has shown that this plant contains bioactive compounds, including phenolics and flavonoids. In this study, bracts and flowers of B. glabra were extracted using ethanol. Folin-Ciocalteu's and aluminum chloride colorimetric assays were used to assess the total phenolic and flavonoid contents, respectively. Anti-diabetic activities were evaluated by α-amylase and α-glucosidase inhibition assays. Glucose uptake of HepG2 hepatocytes and L6 myoblasts was studied. The level of lipid accumulation in 3T3-L1 preadipocytes were assessed. The results revealed that the ethanolic extract of B. glabra exhibited higher inhibition of α-amylase and α-glucosidase activity than voglibose, a positive control. It also showed the higher of glucose uptake in HepG2 and L6. Moreover, ethanolic extract of B. glabra showed a better reduction of lipid accumulation in 3T3-L1 when compared to positive control. The results of the GC-MS analysis showed that the ethanolic extract of B. glabra had a high content of secondary metabolite with anti-diabetic potential. This study showed that the ethanolic extract of B. glabra had anti-diabetic effects, and it could develop for anti-diabetic treatment.

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来源期刊

Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

7.70

自引率

2.50%

发文量

308

审稿时长

48 days

期刊介绍： Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.