Biocatalysis and agricultural biotechnology最新文献_第2页

In-vivo toxicological study of cysteamine modified carbon dots derived from Ruellia simplex on fruit fly for potential bioimaging 半胱胺修饰的碳点在果蝇体内的毒理学研究--用于潜在的生物成像

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-09-12 DOI: 10.1016/j.bcab.2024.103359

{"title":"In-vivo toxicological study of cysteamine modified carbon dots derived from Ruellia simplex on fruit fly for potential bioimaging","authors":"","doi":"10.1016/j.bcab.2024.103359","DOIUrl":"10.1016/j.bcab.2024.103359","url":null,"abstract":"<div>Modern medicine must shift its paradigm from conventional treatment strategies to theranostics to meet individuals' needs. Carbon dots, as a class of fluorescent materials, provide biocompatible and multifunctional solutions for a wide range of applications including clinical sensing, imaging, and drug delivery. Various studies have focused on their synthesis, photophysical properties, and innoxious nature but their potential biomedical applications for the in-vivo toxicity are yet limited. In this work, previously synthesized C-Dots (CDs) were utilized to determine their effect on the development of Drosophila melanogaster. Simultaneously, the genotoxic potential of CDs was evaluated on specific larval cell types that play important roles in immunological defence as well as growth and development. The gut organ toxicity of both CDs was studied using DAPI and DCFH-DA dyes wherein RS-CDs didn't show significant toxicity to the concentration 500 μg/mL whereas RS-Cys-CDs showed nuclear fragmentation and modest ROS (reactive oxygen species) production. Subsequently, trypan-blue assay, larvae crawling assay, touch sensitivity, adult phenotype, and survivability assay were performed. The trypan-blue assay shows the non-toxic nature of both CDs even at the concentration of 500 μg/mL. The high concentrations of RS-Cys-CD (500 μg/mL) were further associated with the alteration in touch behaviour and decrease in pupa hatching. In-vivo and ex-vivo fluorescence assessment of both the CDs exhibit bright fluorescence in green and red channels upon excitation at 485 and 577 nm respectively. The prominent imaging results from RS-Cys-CDs highlight the positive impact of surface modification.</div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring soil microbiota and their role in plant growth, stress tolerance, disease control and nutrient immobilizer 探索土壤微生物群及其在植物生长、抗逆性、疾病控制和养分固定方面的作用

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-09-11 DOI: 10.1016/j.bcab.2024.103358

{"title":"Exploring soil microbiota and their role in plant growth, stress tolerance, disease control and nutrient immobilizer","authors":"","doi":"10.1016/j.bcab.2024.103358","DOIUrl":"10.1016/j.bcab.2024.103358","url":null,"abstract":"<div>Agriculture and the human population have historically thrived together; however, the exponential growth of the human population has now surpassed the capacity of natural resources to meet global food demands. Current agricultural practices are increasingly compromising environmental and ecosystem health. Traditionally, agricultural practices aimed to maximize output with minimal input. However, the relentless drive for higher production, coupled with resource overuse and an insatiable demand for increased yields, has initiated a detrimental cycle, ultimately resulting in the need for increased inputs. This cycle includes diminishing returns, increased input demands, environmental degradation, and a perpetual escalation of input requirements. Specifically, higher input use leads to the repeated application of chemical fertilizers to enhance yields and the expansion of farmland at the cost of natural habitats. This expansion also involves extensive pesticide use to combat diseases, resulting in significant biodiversity loss. To address the rising demands while mitigating health concerns and striving for sustainable agriculture, researchers propose an integrated solution: the use of plant growth-promoting rhizobacteria (PGPR) alongside traditional agrochemicals. Utilizing PGPR in a balanced manner as both bio-fertilizers and bio-pesticides offers a holistic approach to sustainable agriculture. This strategy enhances stress tolerance, promotes growth, increases yields, serves as a biocontrol agent, suppresses diseases, and immobilizes nutrients, all while being eco-friendly. However, bridging the gap between research, formulation, and commercialization of PGPR remains essential for its successful application in agriculture. A comprehensive understanding of microbial ecology, metatranscriptomics, biotechnology, and rhizo-engineering is proposed to optimize the efficacy of these bio-products to their fullest potential.</div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142270918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of trophic mode and medium composition on the biochemical profile and antioxidant capacity of Tetraselmis chuii (CCAP 66/21B) 营养模式和培养基成分对翠玉藻（CCAP 66/21B）生化特征和抗氧化能力的影响

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-09-11 DOI: 10.1016/j.bcab.2024.103362

{"title":"The effects of trophic mode and medium composition on the biochemical profile and antioxidant capacity of Tetraselmis chuii (CCAP 66/21B)","authors":"","doi":"10.1016/j.bcab.2024.103362","DOIUrl":"10.1016/j.bcab.2024.103362","url":null,"abstract":"<div>Microalgal cultivation influences the bioaccumulation of high-value compounds such as omega-3 (ω-3) fatty acids and carotenoid pigments. Therefore, cultivation optimisation is essential to upregulate high-value compound yields. The present study investigated the effects of trophic mode (autotrophy, heterotrophy and mixotrophy) and media composition on the biochemical make-up, pigment signature, fatty acid profile, and antioxidant capacity of the marine chlorophyte, Tetraselmis chuii (CCAP 66/21 B). The 13 conditions significantly affected the biochemical profile of T. chuii (CCAP 66/21 B) with high variation in carbohydrates (78.5–151.7 mg glucose eq g−1dw), lipids (208.3–475.1 mg g−1dw) and soluble proteins (47.3–373.9 mg BSA eq g−1dw). Trophic mode influenced the yields of high-value nutraceutical carotenoids (lutein and β-carotene) with the highest returns observed in photoautotrophic conditions (e.g., 2.51 ± 0.11 mg β-car g−1dw and 1.96 ± 0.14 mg lut g−1dw for K medium). Organic supplementation also induced significant shifts in the proportions of polyunsaturated fatty acids (ω-3 and ω-6 PUFAs), monounsaturated fatty acids (MUFAs) and saturated fatty acids (SFAs). Here, heterotrophy and mixotrophy significantly upregulated MUFA content (33.2 ± 4.0%) and SFA content (31.29 ± 0.9%), respectively. Moreover, mixotrophy significantly enhanced biomass yield (6.3-fold), soluble protein content (3.9-fold) and the antioxidant capacity (5.2-fold) of T. chuii (CCAP 66/21 B) compared to obligate photoautotrophy and heterotrophy. As such, trophic mode is a principal growth parameter that can modulate the content of T. chuii (CCAP 66/21 B) for potential downstream applications such as biofuels, pharmaceuticals, nutracurticals or aquaculture probiotics.</div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycolipopeptide biosurfactant produced by Klebsiella pneumoniae ssp. ozaenae BK34: Virtue in germination and growth of chickpea (Cicer arietinum L.) HC-1 crop 肺炎克雷伯氏菌（Klebsiella pneumoniae ssp. ozaenae BK34）产生的糖脂肽生物表面活性剂：在鹰嘴豆（Cicer arietinum L. ）HC-1作物发芽和生长中的作用

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-09-10 DOI: 10.1016/j.bcab.2024.103353

{"title":"Glycolipopeptide biosurfactant produced by Klebsiella pneumoniae ssp. ozaenae BK34: Virtue in germination and growth of chickpea (Cicer arietinum L.) HC-1 crop","authors":"","doi":"10.1016/j.bcab.2024.103353","DOIUrl":"10.1016/j.bcab.2024.103353","url":null,"abstract":"<div>Modern agriculture consumes many persistent and non-biodegradable agrochemicals enhancing production and economy for farmers. Besides, chemical surfactants are used as adjuvant to augment the action of agrochemicals. A toxicity concern of these agrochemicals and adjuvants on life forms and environment cannot be ignored. Microbiologically produced, eco-friendly, biodegradable surfactants could be suggested as an alternative, however, compatibility with plants and beneficial microflora needs to be ascertained before applying them into agriculture. The current study included evaluation of biosurfactant BS34 produced by Klebsiella pneumoniae BK34 towards germination and growth of chickpea [Cicer arietinum (L.)] seeds. The biosurfactant BS34 was characterized as surfactin like glycolipopeptide based on Fourier transform infrared spectroscopy and Liquid chromatography-Mass spectrometry characterization. The biosurfactant was found non-phytotoxic during in-vitro germination of chickpea seeds as evident from very high germination index of 187.74 as opposed to the almost negligible germination index of 0.04 shown by highly phytotoxic chemical surfactant. In comparison to control, the biosurfactant seed treatment [1%(w/v)] in an in-vivo study on chickpea increased the plant height by 18%, nodule number by 11.13%, nodule fresh weight by 12.66% shoot weight by 11.20% and root weight by 34.61%. Contrarily, seed treatment by 1% (w/v) sodium dodecyl sulfate (SDS) inhibited the germination and growth of chickpea seeds again documenting superiority of biosurfactants over their chemical counterparts. Improved seed germination, growth and yield attributes of chickpea crop in presence of non-phytotoxic glycolipopeptide biosurfactant produced by Klebsiella pneumoniae ssp. ozaenae BK34 suggests its potential as an alternative to chemical surfactants used in agriculture.</div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tailor-made green composts with suppressive properties against tracheofusariosis of wild rocket (Diplotaxis tenuifolia): Useful option for sustainable circular horticulture 量身定制的绿色堆肥对野生火箭菜（Diplotaxis tenuifolia）的气管炎有抑制作用：可持续循环园艺的有益选择

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-09-10 DOI: 10.1016/j.bcab.2024.103357

{"title":"Tailor-made green composts with suppressive properties against tracheofusariosis of wild rocket (Diplotaxis tenuifolia): Useful option for sustainable circular horticulture","authors":"","doi":"10.1016/j.bcab.2024.103357","DOIUrl":"10.1016/j.bcab.2024.103357","url":null,"abstract":"<div>Intensive cultivation systems of wild rocket (Diplotaxis tenuifolia) are threatened by the outbreak of tracheofusariosis caused by the soil-borne fungal pathogen Fusarium oxysporum f. sp. raphani. The disease is difficult to eradicate by curative methods due to the endophytic progression of the pathogen. Suppressive composts can help to prevent this adversity by providing organic matter carrying chemical and biological components that can directly and/or indirectly interfere with the plant-pathogen system. In this study, a collection of seven promising composts from different selected green feedstocks were investigated for their suppressive properties towards wild rocket Fusarium wilting. In planta assays identified two composts, obtained from olive tree pruning + plant and tomato residues, and from olive waste + straw + wool residues, with the highest levels of suppressiveness, influenced by both application dose and plant development stage. These active composts showed overlapping 13C NMR profiles as in vivo suppressiveness, being positively correlated with the organic aliphatic alkyl, O-Aryl and carboxyl C fractions and subsequently with the hydrophobic index and alkyl ratio, and negatively with the lignin ratio. On the contrary, although biotic compost components showed antifungal effects, biological properties did not correlate with in planta wilting suppression. Consistently, reflectance spectroscopy in the Vis-NIR-SWIR highlighted the clustering of samples depending on their feedstock and chemical composition. The results allow the hypothesis of a plant-mediated suppression mechanism. The composting of crop residues improves the circularity of horticulture and makes it possible to produce, from selected matrices, tailored compost towards the sustainable cultivation of wild rocket.</div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142230246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of PGRs, polyamines and potassium to improve pomegranate flowering behaviour, fruit set and fruit quality PGR、多胺和钾对改善石榴开花行为、坐果和果实质量的影响

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-09-10 DOI: 10.1016/j.bcab.2024.103355

{"title":"Impact of PGRs, polyamines and potassium to improve pomegranate flowering behaviour, fruit set and fruit quality","authors":"","doi":"10.1016/j.bcab.2024.103355","DOIUrl":"10.1016/j.bcab.2024.103355","url":null,"abstract":"<div>Ever changing climate is affecting the flowering pattern in pomegranate worldwide. It is the need of the hour to find out some chemicals that can not only enhance hermaphrodite flowers and fruit set but also enhance fruit quality. The present investigation aimed to evaluate the effect of plant growth regulators (three), polyamines (two) and other chemicals (five) on photosynthesis, flowering ratio, fruit set and quality of pomegranate fruits. Pomegranate trees were treated at full leaf expansion stage. Potassium dihydrogen phosphate (5000 ppm), spermidine (1.5 ppm) and naphthalene acetic acid (NAA) (20 ppm) were the best treatments to significantly enhance hermaphrodite flowers, as the hermaphrodite to male flower ratio was 0.39, 0.37, and 0.35, respectively, compared to 0.22 in the control treatment. Except spermidine, NAA and paclobutrazol, all treatments increased the fruit setting significantly, with potassium dihydrogen phosphate (5000 ppm) being the most effective treatment with 66% fruit set compared to 43% in control trees. In mature fruits, compared to control, NAA enhanced total phenols, ascorbic acid and total antioxidants (3%, 15%, 7%, respectively) followed by ascorbic acid treatment (250 ppm); potassium dihydrogen phosphate (5000 ppm) and putrescine (44 ppm) enhanced the anthocyanin content and hence aril color significantly (11% and 10% respectively). Though the use of NAA in pomegranate production is commercial practice, two sprays of ascorbic acid can also be included to enhance the nutritional quality of fruits. Foliar application of potassium dihydrogen phosphate twice at flowering stage could be highly useful for quality pomegranate production due to the enhanced number of hermaphrodite flowers, fruit setting and fruit color. Overall, use of these chemicals can be beneficial to pomegranate farmers to get more remuneration from harvest.</div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142230244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fungi species identified from polluted environment for chromium sequestration and solid state fermentation on tannery shaving waste 从污染环境中鉴定出的真菌物种，用于制革刨花废料的铬固存和固态发酵

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-09-10 DOI: 10.1016/j.bcab.2024.103352

{"title":"Fungi species identified from polluted environment for chromium sequestration and solid state fermentation on tannery shaving waste","authors":"","doi":"10.1016/j.bcab.2024.103352","DOIUrl":"10.1016/j.bcab.2024.103352","url":null,"abstract":"<div>Accumulation of hexavalent chromium in the environment affects our ecosystem. Physico-chemical treatment did not solve the problem, due to high requirement of operational costs & energy, insolubility of Cr (VI) at any pH & sludge accumulation. The study aim is screening and evaluating fungi for chromium biosorption for mycoremediation. Soil and effluent sample were collected. PDA media was used for fungi purification. Fungal chromium tolerance evaluation and optimization was conducted at pH (4, 7, 10), T0 (25, 27.5, 35 °C), Cr concentration (5000–25000 ppm) using Full Factorial experimental design. Spore count, biomass production, Cr tolerance index, mycelia growth were evaluated. Flame Absorptive Spectrophotometer was used for quantification of fungal chromium removal. Fungi species were identified by MALDI-TOF. Fungi growth & degradation of tannery shaving waste was evaluated. The result revealed 6 potential fungi, T. longibrachiatum, T, koningii, A. niger, G. candidum, P. rubens, T. orientale were identified. Optimium mean biomass & spore count were 0.514 ± 0.051& 1.39 ± 0.038 at pH4 and 27.5 °C respectively. MTL test indicate that Trichoderma species, A. niger & P. rubens survive up to 10,000 ppm, 15, 000 ppm & 20,000 ppm respectively. FAAS analysis of fungi chromium removal from aqueous media result indicate that A. niger (99%). T. longibrachiatum (85%) and P. ruben (84.6%). These 6 fungi are efficient in tannery shaving waste degradation but A. niger was superior in spore count 15.1 Log/mL and 7 g substrate weight loss. In conclusion three fungi have high chromium tolerance and removal capacity and shaving waste degradation promising for mycoremediation.</div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic insights on the antibiofilm potential of snake venom peptides: An in silico based molecular docking approach 蛇毒多肽抗生物膜潜力的机理研究：基于硅学的分子对接方法

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-09-07 DOI: 10.1016/j.bcab.2024.103351

{"title":"Mechanistic insights on the antibiofilm potential of snake venom peptides: An in silico based molecular docking approach","authors":"","doi":"10.1016/j.bcab.2024.103351","DOIUrl":"10.1016/j.bcab.2024.103351","url":null,"abstract":"<div>The threat of antibiotic resistance to the public's health is exacerbated by the emergence of multidrug resistant bacterial (MDR) strains and their biofilm. Antibiotics are unable to penetrate the thick biofilm matrix causing the indwelling bacterial cells to survive resulting into the origin of antimicrobial resistance (AMR). Thus, biofilms are responsible for causing many chronic infections such as dental plaque formation, cystic fibrosis, urinary tract infections (UTI), otitis media etc. Hence, there is an urgent need for alternate therapeutic strategies for preventing bacterial biofilm formation and fighting AMR. In this regard antimicrobial peptides (AMPs) that have broad-spectrum antibacterial action with significant specificity and low toxicity, are a great choice to combat AMR. Peptides derived from snake venom such as cathelicidins reflects a spectrum of biological actions including its antimicrobial potential. The present study focuses on screening and identifying AMPs having anti-biofilm properties. This includes natural cathelicidins found in snake venom as a potential antibacterial agent to combat AMR. Two biofilm forming proteins were identified viz. icaC protein of Staphylococcus aureus & bdlA protein of Pseudomonus aureginosa. AMPs were screened from DRAMP Database and snake venom cathelicidins were selected for further studies. The binding interaction of cathelicidins with the icaC and bdlA protein were investigated using molecular docking studies. Based on the interacting amino acid residues, antigenic determinants, peptide binding site and docking scores a performance scale was built to classify relevant snake venom cathelicidins according to their importance for further in vivo and in vitro antibiofilm investigations.</div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the potential of aqueous enzymatic extraction of safflower (Carthamus tinctorius L.) seed oil: Process optimization and oil characterization 红花（Carthamus tinctorius L.）籽油水酶提取潜力研究：工艺优化和油脂特征

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-09-07 DOI: 10.1016/j.bcab.2024.103354

{"title":"Investigating the potential of aqueous enzymatic extraction of safflower (Carthamus tinctorius L.) seed oil: Process optimization and oil characterization","authors":"","doi":"10.1016/j.bcab.2024.103354","DOIUrl":"10.1016/j.bcab.2024.103354","url":null,"abstract":"<div>Safflower seed oil was extracted by aqueous enzymatic extraction (AEE) with a combination of three enzymes: hemicellulase, pectinase, and protease. A Response Surface Methodology (RSM) was performed to optimize the operating conditions: Temperature, pH, and incubation time. Analysis of variance allowed studying the variables' linear, quadratic, and interaction effects. The optimized extraction conditions resulted in 85% oil recovery referring to the conventional Soxhlet extraction (SE). Subsequently, a comparative analysis was conducted to evaluate the quality and composition of oils obtained by SE and AEE. The results revealed that the two oils had similar values of peroxide index, conjugated dienes and trienes, and fatty acid composition. AEE-oil had a lighter yellow color (L* = 31.8 Vs. 24.7) and was richer in polyphenols (147 Vs. 101 mg GAE/Kg of oil), but it was more acidic (2.75% Vs. 1.56% linoleic acid) and less stable (2.86h Vs. 5.66h induction time) with less tocopherol (302 Vs. 409 mg/kg oil) and carotenoid content (0.42 Vs. 0.57 mg/kg oil) than SE-oil. Our findings demonstrated that RSM-based optimization is an efficient and time-saving approach, which significantly improved oil extraction. Finally, we suggested several solutions to further improve the quality and quantity of the obtained AEE-safflower seed oil, paving the path for other future research possibilities.</div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142171824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing fruit quality and yield in tomato through cyanobacterium mediated nutri-fertigation 通过蓝藻介导的营养灌溉提高番茄果实的质量和产量

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-09-07 DOI: 10.1016/j.bcab.2024.103344

{"title":"Enhancing fruit quality and yield in tomato through cyanobacterium mediated nutri-fertigation","authors":"","doi":"10.1016/j.bcab.2024.103344","DOIUrl":"10.1016/j.bcab.2024.103344","url":null,"abstract":"<div>Protected cultivation of high-value crops such as tomato, with several health benefits, is gaining global significance. This study explored the influence of aqueous formulations of cyanobacteria-Anabaena laxa (C11), Nostoc carneum (BF2), and Anabaena doliolum (BF4), applied as soil drench at pre-flowering and fruiting stages, on the dynamics of soil and plant attributes important for growth, productivity, and quality of fruits in an indeterminate tomato variety (NS4266) under protected cultivation. Drenching with the BF2 formulation resulted in a significant fold-increase in soil dehydrogenase activity and an increase of 40–45% in soil nitrogen availability; positively impacted glutamine synthetase activity, fruit weight, lycopene content, skin elasticity, in comparison to control. C11 drenching led to a 17% elevation in soil microbial biomass carbon, besides bringing about a 24 and 54% enhancement in available phosphorus and iron content. Additionally, it also enhanced chlorophyll a, b and total pigments by 54–65% over control. Principal Component Analysis (PCA) and Multivariate analysis showcased the distinct effects of cyanobacterial formulations on crop growth and soil fertility, as well as the significant and positive correlations among quality, plant, soil, and yield attributes contributed through cyanobacterial drenching. This investigation highlighted cyanobacterial drench as a promising organic option to augment soil nutrient availability, bolster overall productivity, and enhance fruit quality in tomato grown under protected cultivation.</div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0