Biocatalysis and agricultural biotechnology最新文献

{"title":"Transesterification of castor oil and ethanol using green catalyst for biodiesel production through Box-Behnken design","authors":"Amit Kumar Rajak ,&nbsp;Madiga Harikrishna ,&nbsp;Devi Lal Mahato ,&nbsp;Urupasala Anandamma ,&nbsp;Ramyakrishna Pothu ,&nbsp;Rajender Boddula ,&nbsp;Rajesh Kumar Srivastava ,&nbsp;Mallampalli S.L. Karuna ,&nbsp;Prakash Kumar Sarangi","doi":"10.1016/j.bcab.2024.103480","DOIUrl":"10.1016/j.bcab.2024.103480","url":null,"abstract":"<div><div>Globally, there's a noticeable decline in environmental pollution, largely attributed to the widespread adoption of biodiesel a sustainable alternative fuel derived from vegetable oils and animal fats. This study explores an innovative approach where castor oil and ethanol undergo trans-esterification using waste potato peels infused with calcium oxide (CaO) as a catalyst. The process begins with calcining the potato peels at 600 °C for 4 h, followed by combining the residual peels with CaO and subjecting them to further calcination at 900 °C for 3 h. SEM-EDX analysis confirms the successful impregnation of CaO onto the potato peels, resulting in CaO-treated potato peels (CaO-CPP) with a smoother surface area compared to untreated CaO. Gas chromatography mass spectrometry is employed to analyze the fatty acid esters within biodiesel. Establishing biodiesel as a viable alternative fuel involves evaluating various parameters, including specific gravity, moisture content, kinematic viscosity, saponification value, iodine value, flash point, and heat content. The characteristics of biodiesel suggest that, with appropriate adjustments, biodiesel derived from castor seed oil holds promise as an alternative fuel source. Response Surface Methodology is utilized to optimize the operational parameters of the production process. Through a Box-Behnken Design, the impact of catalyst type, concentration, and the ratio of oil to ethanol on biodiesel yield is examined. Statistical analysis reveals the model's high significance, with an R^{<strong>2</strong>} value of 0.9930. Optimal conditions are achieved within 60 min, resulting in a 94.17% biodiesel yield at a 1:9 oil to ethanol molar ratio, with a 3.5% catalyst concentration by weight, and a temperature of 65 °C. Impregnated CaO utilizing CPP (CaO-CPP) emerges as a promising catalyst for heterogeneous bases, thanks to its robust catalytic activity, which significantly facilitates the transesterification process.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103480"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioconversion of oil palm empty fruit bunches waste into prebiotic xylooligosaccharides using a genetic modified cross-linked endoxylanase aggregates immobilization","authors":"Nashriq Jailani,&nbsp;Nardiah Rizwana Jaafar,&nbsp;Nur Izyan Wan Azelee,&nbsp;Roshanida A. Rahman,&nbsp;Rosli Md Illias,&nbsp;Mohd Khairul Hakimi Abdul Wahab","doi":"10.1016/j.bcab.2025.103489","DOIUrl":"10.1016/j.bcab.2025.103489","url":null,"abstract":"<div><div>Recent progress has been achieved towards more environmentally friendly and productive approaches using xylanolytic enzyme in producing prebiotic xylooligosaccharides (XOS) from inexpensive lignocellulosic biowaste. The cost of hydrolyzing XOS from appropriate materials high in xylan and the lack of xylanase enzyme activity after several treatment cycles are among the most pressing problems faced by the industries. In this study, XOS was produced utilizing a cross-linked mutated endoxylanase aggregates (CLmXynA) immobilization from an oil palm empty fruit bunches (OPEFB) waste. OPEFB was pretreated using sodium hydroxide-peracetic acid two-stage process which successfully removed 61.1% of lignin and 78.4% of extractives. From 28.4% of hemicellulose recovered, the highest hydrolysis activity (19.3%) was optimized using one-factor at one time approach where a total of 11.01 mg/g of XOS was produced. X3 (70.5%) was shown to be a major product meanwhile, this study presented longer XOS oligomers (X4 = 5.1% and X5 = 6.1%) hydrolyzed compared to a common major of X2 and X3 from a previous report. Moreover, CLmXynA maintained 29% of its initial XOS hydrolysis after five cycles, each of 48 h of reaction and depicted 1.6-fold higher activation energy (33.1 kJ mol⁻¹ K⁻¹) than its apparent kinetic model when the heterogenous kinetic hydrolysis were assessed using a modified Prout-Tompkin equation. As a conclusion, immobilized CLmXynA shown to be an efficient and stable biocatalyst while OPEFB which is a markedly large agriculture biowaste produced especially in Southeast Asia demonstrated a promise alternative of low-cost natural substrate for XOS production as a valuable prebiotic.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103489"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recuperating agricultural wastes into feed additives","authors":"Sukhendu Dey ,&nbsp;Megha Santra ,&nbsp;Apurba Ratan Ghosh ,&nbsp;Palas Samanta","doi":"10.1016/j.bcab.2025.103508","DOIUrl":"10.1016/j.bcab.2025.103508","url":null,"abstract":"<div><div>Every year, millions of tonnes of agricultural waste (AWs) are produced globally. Agricultural wastes are of poorer quality than the primary products, yet they have similar characteristics. To reduce environmental stress, managing these agricultural wastes is expensive and calls for stringent regulations. As a result, turning these agricultural wastes into useful goods minimizes negative effects on the environment, supports the agricultural sector, and enriches industry participants. Recent decades have witnessed an enormous amount of study on the synthesis of cutting-edge biologically active compounds and an array of bioproducts from agricultural waste owing to the prospective importance of crop residue in the agri-food-pharma domains through diverse valorisation approaches. Accordingly, the present study demonstrated agricultural waste generated diverse value-added feed bioproducts as a feed supplement to enhance animal fitness and boosting output, as well as current state of various agri-waste valorisation techniques. The study also covers the application areas of these feed additives.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103508"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143148747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity of cultivated Nigella sativa L. germplasm based on EST-SSR markers and agro-morphological traits","authors":"Vahid Fozi ,&nbsp;Hassan Esmaeili ,&nbsp;Samad Nejad Ebrahimi ,&nbsp;Mohammad Hossein Mirjalili ,&nbsp;Frank Marthe ,&nbsp;Maryam Pezhmanmehr","doi":"10.1016/j.bcab.2025.103498","DOIUrl":"10.1016/j.bcab.2025.103498","url":null,"abstract":"<div><div><em>Nigella sativa</em> L. (Black cumin) has been extensively utilized in traditional medicine since ancient times. Researching genetic resources to identify the best germplasm for enhancing pharmaceutical properties is an important step in maximizing the potential of this beneficial plant. In this study, EST-SSR markers were designed and investigated alongside thymoquinone content and some important agro-morphological traits e.g., seed yield. After <em>de novo</em> transcriptome sequencing using the Illumina platform, data mining was performed to identify unigenes containing microsatellites using MISA software. Among 50 designed EST-SSR primers, 19 pairs reproduced polymorphic bands which were employed to estimate the genetic diversity of 32 accessions of black cumin provided from across the world. A total of 117 bands (75%) were polymorphic among 156 amplified bands. The PIC values varied from 0.47 (NS-587 and NS-183) to 0.69 (NS-242). The assessment of phytochemical variability and yield traits revealed a thymoquinone content of 0.1–1.38 %, a thousand-seed weight of 1.7–3.78 g, and a seed yield per plant of 0.28–1.74 g. Classification of accessions based on morphological traits resulted in three distinct clusters, while genetic analysis yielded four clusters. Notably, the high concordance between the morphological and genetic dendrograms indicated that the genetic loci targeted by EST-SSRs in this study can effectively represent key morphological traits. This study yielded valuable insights into the genetic diversity status of <em>Nigella sativa</em>, while also introduced some accessions for further breeding endeavors.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103498"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143148752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling multifaceted bioactivity assessment of Psidium guajava and Azadirachta indica leaves extract as a potential natural tyrosinase inhibitors","authors":"Faiza Nazir ,&nbsp;Zaveeba Jabeen ,&nbsp;Farheen Aslam ,&nbsp;Osama A. Mohammed ,&nbsp;Naveed Ahmad ,&nbsp;Shahid Iqbal ,&nbsp;Arif Nazir ,&nbsp;Munawar Iqbal","doi":"10.1016/j.bcab.2024.103486","DOIUrl":"10.1016/j.bcab.2024.103486","url":null,"abstract":"<div><div>Tyrosinase is a critical enzyme involved in melanin production, playing a protective role against harmful ultraviolet radiation from the sun. However, excessive melanin synthesis can lead to dermatological issues, including skin irritation, redness, and hyperpigmentation. These conditions can be mitigated by employing tyrosinase inhibitors. Studies have demonstrated that aqueous extracts of <em>Psidium guajava</em> (guava) leaves and <em>Azadirachta indica</em> (neem) leaves exhibit significant tyrosinase-inhibitory activity. Specifically, <em>Psidium guajava</em> extracts have inhibition rates ranging from 68.58% to 79.51%, while <em>Azadirachta indica</em> extracts display 52.34%–56.48% inhibition rates. The antioxidant capacity of these extracts is closely linked to their total phenolic content. Fourier Transform Infrared Spectroscopy (FT-IR) analysis indicates that <em>Psidium guajava</em> extracts are abundant in polyphenols, including tannins and flavonoids, which contribute to their superior tyrosinase inhibition compared to <em>Azadirachta indica</em>. Moreover, GC-MS and HPLC analyses have confirmed the presence of biologically active compounds such as quercetin, gallic acid, vanillic acid, hydroquinone, caffeic acid, chlorogenic acid, rutin, catechin, sinapic acid, and ferulic acid, supporting the extracts’ anti-tyrosinase properties. Interestingly, the concentration of quercetin—a compound known for its antibacterial properties—is higher in <em>Azadirachta indica</em> leaves (45.42 ppm) than in <em>Psidium guajava</em> leaves (41.18 ppm). This higher quercetin content may explain why <em>Azadirachta indica</em> extracts demonstrate greater antibacterial activity despite their lower tyrosinase inhibition potential compared to <em>Psidium guajava</em> extracts.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103486"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteases from marine endophyte, Bacillus subtilis ULB16: Unlocking the industrial potential of a marine-derived enzyme source","authors":"Swathy Sadanandan Anand,&nbsp;Bipin Gopalakrishnan Nair,&nbsp;Sudarslal SadasivanNair,&nbsp;Jayashree GopalakrishnaPai","doi":"10.1016/j.bcab.2025.103503","DOIUrl":"10.1016/j.bcab.2025.103503","url":null,"abstract":"<div><div>A potential protease producer, exhibiting 98% genetic similarity to <em>Bacillus subtilis</em> LXB3, was isolated from a marine macroalga, <em>Ulva lactuca</em>, and is extensively studied for its potential to produce proteases. This research delved into the exoenzyme profile of the isolate, with a particular emphasis on its significantly higher protease activity when compared to a reference strain, <em>Bacillus subtilis</em> 168. Ammonium sulfate precipitation of the spent culture media revealed that the 60–80% fraction (active protease fraction) exhibited the highest protease content and activity. Mass spectrometric characterization identified multiple alkaline proteases within this sample. The protease fraction displayed optimal activity at 50 °C and pH 10, with notable enhancements in the presence of divalent cations such as Mg²⁺ and Ca²⁺. Significant kinetic parameters of the active fraction with casein substrate suggest its potential to be explored for its different industrial applications. The specific inhibition of protease activity by phenylmethylsulfonyl fluoride indicated a predominance of serine proteases in the active protease fraction. Beyond protease activity, the consortium of proteins also revealed significant biosurfactant properties. This dual functionality underscores the isolate's potential for biofilm inhibition and effective removal of persistent stains, such as blood, in textile applications and also its eligibility to be used in oil spill bioremediation. The current study highlights the newly identified marine endophytic bacterium, <em>Bacillus subtilis</em> ULB16, as a potent and versatile source of industrial enzymes, demonstrating notable protease activity.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103503"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"β-Glucosidase from Lacticaseibacillus casei TISTR 1463: Biochemical characterization and application on biotransformation of pigmented leaf extract from Oryza sativa L. (Luem Pua glutinous rice)","authors":"Sirinthip Jaijoi ,&nbsp;Tanyawat Kaewsalud ,&nbsp;Kamon Yakul ,&nbsp;Sugunya Mahatheeranont ,&nbsp;Woraprapa Sriyotai ,&nbsp;Sarana Rose Sommano ,&nbsp;Pornchai Rachtanapun ,&nbsp;Noppol Leksawasdi ,&nbsp;Masanori Watanabe ,&nbsp;Thanongsak Chaiyaso","doi":"10.1016/j.bcab.2025.103491","DOIUrl":"10.1016/j.bcab.2025.103491","url":null,"abstract":"<div><div>This study aimed to biochemically characterize a purified β-glucosidase from a probiotic <em>Lacticaseibacillus casei</em> TISTR 1463 and to apply the enzyme for improving antioxidant activities of Luem Pua (<em>Oryza sativa</em> L.) pigmented rice leaf extract (PRLE-LP). The effect of PRLE-LP supplementation in de Man, Rogosa, and Sharpe (MRS) medium, inoculum size, initial pH, and temperature were investigated using one-factor-at-a-time (OFAT) approach. Under optimal conditions, the maximal β-glucosidase activity of 38.43 ± 0.01 U/g _DCW was achieved by cultivation strain TISTR 1463 in MRS-PRLE-LP medium (20:80% (v/v)), 10% (v/v) inoculum size, initial pH 3.5 at 30 °C for 36 h. Afterward, β-glucosidase was purified to 7.5-fold with 37% recovery yield and a molecular weight (MW) of 75 kDa. This purified enzyme had an optimal pH and temperature of pH 4.5 and 35 °C. It was stable under pH of 3.0–5.0 and temperature of 30–35 °C and showed the highest specific activity toward 4-nitrophenyl β-D-glucopyranoside (<em>p</em>-NPG) with the <em>K</em>_<em>m</em> and <em>V</em>_max of 1.31 mg/mL and of 0.06 μmol/min/mg. The enhancement of antioxidant activities of PRLE-LP by purified β-glucosidase from strain TISTR 1463 and commercial enzyme was also studied. An in-house β-glucosidase displayed superior antioxidant activities over the commercial enzyme from <em>Aspergillus niger</em>. In addition, LC-QTOF-MS analysis confirmed that β-glucosidase efficiently converted glycone into aglycone, resulting in enhanced antioxidant activities. The potential for producing antioxidant-rich substances from anthocyanin-containing alternative crops with applications in food and pharmaceutical industries that are both health and eco-friendly is thus addressed.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103491"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofertilizers as an eco-friendly approach to combat drought stress in plants","authors":"Zhenqi Liao ,&nbsp;Hatem Boubakri ,&nbsp;Beibei Chen ,&nbsp;Muhammad Farooq ,&nbsp;Zhenlin Lai ,&nbsp;Hongtai Kou ,&nbsp;Junliang Fan","doi":"10.1016/j.bcab.2025.103510","DOIUrl":"10.1016/j.bcab.2025.103510","url":null,"abstract":"<div><div>Drought is a severe abiotic stress adversely affecting agricultural activity worldwide. Biofertilizers have emerged as an environmentally-safe tool to combat drought adverse effects in plants. Biofertilizers like endophytic fungi (EF), arbuscular mycorrhizal fungi (AMF), plant growth-promoting bacteria (PGPB) and natural compounds have enormous potential to mitigate drought negative effects in plants. Microbials like PGPB and AMF improve physical, chemical and biological features of soils by promoting nutrient uptake and water flow. However, natural compounds provide drought stress tolerance by inducing morphological and structure changes, water status adjustment, stomatal regulation, osmotic regulation, ion balance and pH regulation, reactive oxygen metabolism, antioxidant defense system, photosynthetic system, plant hormones, signal transduction, and gene expression regulation. This review provides valuable background knowledge on various biofertilizers that have proven effective against drought and serves as theoretical basis for conducting future research under field conditions to validate their practical use.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103510"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143148750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of medium formulations for biomass vaccine production of gdhA derivative Pasteurella multocida B:2 using statistical experimental design","authors":"Siti Nur Hazwani Oslan ,&nbsp;Jiun Shen Loo ,&nbsp;Rosfarizan Mohamad ,&nbsp;Siti Khairani Bejo ,&nbsp;Mohd Zamri Saad","doi":"10.1016/j.bcab.2025.103504","DOIUrl":"10.1016/j.bcab.2025.103504","url":null,"abstract":"<div><div>The glutamate dehydrogenase (gdhA) gene of a pathogenic <em>Pasteurella multocida</em> B:2 was successfully inactivated to create an attenuated strain as a vaccine candidate against hemorrhagic septicemia (HS) in ruminants. This study presents a novel approach to optimizing the medium formulation for high-throughput mass production of a gdhA-inactivated <em>P. multocida</em> B:2, which is critical for scaling up vaccine production. Using response surface methodology (RSM) with a central composite design (CCD), we systematically investigated the effects of various medium components on biomass yield. Yeast extract, glucose, sodium chloride, and sodium phosphate were identified as critical factors, with yeast extract demonstrating a significant enhancement in biomass production, yielding 2.03 ± 0.15 mg/mL, compared to traditional peptone (1.30 ± 0.26 mg/mL) and inorganic nitrogen sources like ammonium chloride and ammonium sulfate (&lt;1.0 mg/mL). Among carbon sources, glucose paired with yeast extract produced the highest biomass, while sucrose, white sugar, and soluble starch had minimal effects. Optimization through CCD identified the ideal concentrations of yeast extract, glucose, NaCl, and NaH₂PO₄ as 15.64 g/L, 1.91 g/L, 3.06 g/L, and 2.48 g/L, respectively, resulting in a 35% increase in biomass yield to 3.10 mg/mL. Yeast extract was the key driver of growth, with optimal concentrations between 5 and 20 g/L, while excess glucose, NaCl, and NaH₂PO₄ inhibited growth. Statistical analysis revealed that the quadratic polynomial model fit the data well (R² = 0.8440, model F-value = 5.80, p &lt; 0.05). The novelty of this study lies in the optimization of a medium that significantly improves biomass production compared to conventional formulations, providing a cost-effective and efficient solution for scaling up the production of attenuated <em>P. multocida</em> B:2. This optimized medium holds promise for advancing vaccine development against HS.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103504"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143264431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of Polyphenolic Compound Production from Rubus adenotrichos Schltdl. in a stirred tank bioreactor using two different impellers","authors":"Alexander Schmidt-Durán ,&nbsp;Laura A. Calvo-Castro ,&nbsp;Oscar Acosta-Montoya ,&nbsp;Mario Rodríguez-Monroy","doi":"10.1016/j.bcab.2025.103516","DOIUrl":"10.1016/j.bcab.2025.103516","url":null,"abstract":"<div><div><em>Rubus adenotrichos</em> Schltdl. has a high content of polyphenolic compounds with antioxidant properties; however, obtaining these from the fruits presents limitations because it depends on the plant physiology, developmental stage, and environmental conditions. Cell cultures in a stirred tank bioreactor (STR) offer an alternative for obtaining plant secondary metabolites. The objective of this study was to evaluate the effect of two impellers with different mixing patterns, a marine impeller, and a Rushton turbine impeller, on the culture of <em>R. adenotrichos</em> cells and the production of gallic acid, vanillin and ellagic acid, using an STR. It was observed that the marine impeller enhanced cell growth, reaching 2.320 g L⁻¹ of dry biomass, but did not promote the accumulation of polyphenolic compounds, producing 0.528 mg L⁻¹. Conversely, with the Rushton turbine, cell growth was limited (1.680 g L⁻¹ dry biomass), but the production of the target metabolites was favored, producing a total of 1.043 mg L⁻¹. The decrease in biomass and the increase in specialized metabolite content may be associated with smaller eddy sizes produced by the Rushton turbine, leading to the activation of lethal and sublethal mechanisms in plant cells due to hydrodynamic stress. Additionally, thermal concentration treatments differentially affected each analyzed metabolite, with gallic acid being the least affected compound when the concentration temperature was increased. The results establish the basis for the bioprocess advancement of <em>R. adenotrichos</em> Schltdl., focused on developing a sustainable strategy for natural resource management and as a source of phenolic compounds on a large scale.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103516"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143348682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}