Biocatalysis and agricultural biotechnology最新文献

{"title":"Deep small-RNA sequencing uncovers a diverse spectrum of microRNAs putatively regulating biosynthesis and variation of specialized metabolites in Thymus daenensis","authors":"Hosein Ahmadi ,&nbsp;Reza Fatahi ,&nbsp;Peter Poczai ,&nbsp;Zabihollah Zamani ,&nbsp;Majid Shokrpour","doi":"10.1016/j.bcab.2025.103550","DOIUrl":"10.1016/j.bcab.2025.103550","url":null,"abstract":"<div><div><em>Thymus daenensis</em> Celak., is an ideal model for delving into the intricate regulatory mechanisms involved in polyphenol biosynthesis and thymol-rich essential oil (EO) production. While, there has been an effort to explore metabolic pathways in thyme species at mRNA level, but mechanisms responsible for post-transcriptional control, in particular microRNAs (miRNAs), are understood only poorly. Here, the sequencing of approximately 75 million small-RNA reads resulted in identification of 618 conserved miRNAs, 50 multi-member families, and 28 novel miRNAs. A comparative analysis of the small-RNAome profile was conducted between two distinct genotypes: Zagheh-11, characterized by low EO content but rich in carvacrol and triterpenic acids, and Malayer-21, which exhibited higher levels of thymol, EO, and rosmarinate. The divergence in their terpenome appears to stem from a genotype-specific control over MEP and MVA pathways, primarily influenced by 10 differentially expressed miRNAs. The expression patterns of key miRNAs and their putative targets ([miR396c, DXS], [miR477a, ispD], [miR135a, ispE], [miR9b-5p, MVD and MVK], [miR181a-3p, ACAT]) were validated using qRT-PCR. Furthermore, we uncovered an extensive network consisting of 139 miRNAs that target 47 enzyme genes in the biosynthetic pathways of secondary metabolites. The majority of the 13 928 identified target unigenes were enriched in pathways of secondary metabolites, carbon and amino acid metabolism. We found miR166 and miR159 as the most abundantly expressed miRNAs in <em>T. daenensis</em>. We examined the conservation and phylogenetic status of the miR159, miR164, and miR396 families, highlighting their profound impacts on production of specialized metabolites. The putative regulatory behavior of <em>tda-</em>miRNAs herein presents a promising foundation for strategically altering metabolic pathways, enabling the controlled production of targeted drug compounds.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103550"},"PeriodicalIF":3.4,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143619146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serratia rubidaea SR19: A cadmium -tolerant bacteria enhancing phosphate solubilization, IAA production, and promoting cucumber seed germination","authors":"Alaa M. El-Minisy ,&nbsp;Shawky A. Bekheet ,&nbsp;Salah El-Din El-Assal ,&nbsp;Mohammed Soliman ,&nbsp;Ahmed M. Amer ,&nbsp;Mohammed Hassan ,&nbsp;Hattem M. El-Shabrawi ,&nbsp;Adel El-Tarras","doi":"10.1016/j.bcab.2025.103546","DOIUrl":"10.1016/j.bcab.2025.103546","url":null,"abstract":"<div><div>Cadmium (Cd) contamination in soil poses significant environmental and health risks due to its toxicity. A sustainable way to detoxify heavy metals from soil is to use rhizobacteria that promote the growth of plants (PGPR). In this study, we measured the production of IAA and the solubility of inorganic phosphate to evaluate the plant growth-promoting traits of four isolates that show cadmium tolerance. None of the four isolates grew at 300 ppm cadmium chloride, indicating that this concentration represents the Minimum Inhibitory Concentration (MIC) for all isolates. Based on 16S rRNA molecular identification, four isolates are classified as <em>Serratia rubidaea.</em> The B3 isolate was selected for whole genome sequencing and deposited in the Japanese database as SR19 to clarify the genetic basis of features that promote plant growth and cadmium tolerance. Annotation revealed the presence of genes linked to heavy metal resistance, stress defense response, siderophore biosynthesis, nitrogen metabolism, sulfur metabolism, and phosphate solubilization. Additionally, we found that under both normal and cadmium stress conditions, <em>S. rubidaea</em> SR19 promoted the germination of cucumber seeds and the growth of seedlings <em>in vitro</em>. Our study suggests that using the isolated strain <em>S. rubidaea</em> SR19 may help mitigate the harmful effects of cadmium and other heavy metals in contaminated agricultural soil.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103546"},"PeriodicalIF":3.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143591485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nitrogen ameliorates NaCl toxicity in eggplant seedlings: Role of hydrogen sulphide and sulphur metabolic signaling","authors":"Madhulika Singh ,&nbsp;Pratibha Singh ,&nbsp;S. Shweta ,&nbsp;Utkarsha Singh ,&nbsp;Sheo Mohan Prasad","doi":"10.1016/j.bcab.2025.103543","DOIUrl":"10.1016/j.bcab.2025.103543","url":null,"abstract":"<div><div>This study investigates physiological and biochemical adaptation of eggplant (<em>Solanum melongena</em> L.) seedlings to sodium chloride (NaCl₀: 0.0 g NaCl kg⁻¹ sand, NaCl₁: 0.3 g NaCl kg⁻¹ sand and NaCl₂: 0.5 g NaCl kg⁻¹ sand) by varied levels of nitrogen (N): deprived N (N₀; 0 mg kg⁻¹ sand), low nitrogen (LN; 105 mg N kg⁻¹ sand), medium nitrogen (MN; 210 mg N kg⁻¹ sand) and high nitrogen (HN; 270 mg N kg⁻¹ sand) supplementation. Sodium chloride at both doses (MN + NaCl₁ and MN + NaCl₂) caused considerable decrease in growth (10 and 14 % in FW and 16 and 21 % in DW, respectively), pigments, photosynthetic oxygen evolution, PSII photochemistry and nutrient contents as compared to control (MN + NaCl₀), while HN supplementation significantly alleviated NaCl toxicity. Under similar treatment, stimulatory effect occurred on sulphur cycle enzymes adenosine triphosphate sulphurylase and o-acetylserine(thiol)lyase and metabolites glutathione and cysteine. NaCl stress induced impact was more severe in LN and N₀ (deprived N) treated seedlings, while HN supplementation protected against damage by up-regulating antioxidant system and components of S cycle. Growth showed negative correlation with Na⁺ accumulation. Nitrogen supplementation could be a potential method to minimize NaCl toxicity in eggplants by up-regulating endogenous H₂S and S metabolites. Further, non-metric-multidimensional scaling provided strong support to represent a spatial model in different variables. This technique is cost effective and covers holistic approach to propose number of agricultural and biotechnological means for sustainable agriculture and human welfare in turn.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103543"},"PeriodicalIF":3.4,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143552259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-assisted biodiesel production from Peltophorum pterocarpum oil: A comparative analysis of prediction accuracy between RSM and ANFIS","authors":"Umaiyambika Neduvel Annal ,&nbsp;Mary Sahaya Anisha John Bosco ,&nbsp;Raman Gurusamy ,&nbsp;Paskalis Sahaya Murphin Kumar ,&nbsp;Mohd Afzal ,&nbsp;Pankaj Khurana ,&nbsp;Mathivanan Durai","doi":"10.1016/j.bcab.2025.103545","DOIUrl":"10.1016/j.bcab.2025.103545","url":null,"abstract":"<div><h3>Background</h3><div>Biodiesel is recognized as a sustainable alternative to conventional fossil diesel. The use of ultrasound energy in biodiesel production enhances reaction efficiency and reduces costs. This study identifies a new feedstock, <em>Peltophorum pterocarpum</em> (commonly known as copper pod seeds), for biodiesel production. In recent times, machine learning (ML) techniques have been employed to predict the biodiesel yield.</div></div><div><h3>Methods</h3><div>Ultrasound assisted transesterification process was utilized for the production of biodiesel from the extracted <em>Peltophorum pterocarpum</em> (Pp) oil. Probe sonicator was used for FAME production. Calcium oxide catalyst derived from waste <em>Pyrgostylus striatulus</em> shells was used as the catalyst. The functional groups present in the extracted oil was characterized using FT-IR analysis. The fatty acid profiling of extracted Pp oil was performed using Gas Chromatography Mass Spectrometry analysis. The research employed ML algorithm systems, specifically Response Surface Methodology (RSM) and Adaptive Neuro Fuzzy Inference System (ANFIS), to analyze biodiesel production. Central Composite Design (CCD) was utilized to optimize operating parameters, including the methanol to oil ratio (9–15 mol/mol), catalyst loading (3–5 wt%), and ultrasonication time (30–60 min). The biodiesel produced was characterized using FT-IR and ¹H NMR instrumentation techniques.</div></div><div><h3>Significant findings</h3><div>The fatty acid composition rom GC-MS analysis of the Copper pod oil revealed that it contains 42.6% linoleic acid, 21.2% oleic acid, and 19.4% palmitic acid. FT-IR analysis confirmed the presence of functional groups, specifically carboxylic acids. This extracted oil was hence suitable for the transesterification process. The best yield of biodiesel from the extracted oil was observed to be 98.6 wt % at 12 mol/mol methanol to Pp oil molar ratio, 4 wt % of CaO and 45 min of ultrasonication time by ANFIS model. Characterization of biodiesel produced was validated through ¹H NMR and FT-IR analysis. The important physical and chemical properties of the biodiesel were analyzed and were found to be within standard limits, indicating its commercial viability. The interpretation of both RSM and ANFIS models were analyzed statistically based on their predicted data by Coefficient of determination, Root mean square error, Standard error of prediction and mean relative percent deviation. The Goodness of fit R² value calculated for RSM and ANFIS models was 0.954 and 0.999 respectively. Both the models have performed well but comparatively ANFIS model had been more accurate proving ANFIS as a potent tool for modelling and optimization of biodiesel production.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103545"},"PeriodicalIF":3.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative physicochemical, microbiological, pharmacological, and toxicological evaluations of different varieties of honey targeting wound repairment","authors":"Kankan Roy ,&nbsp;Ankit Majie ,&nbsp;Bhawana Jha ,&nbsp;Amrita Chatterjee ,&nbsp;Wei Meng Lim ,&nbsp;Bapi Gorain","doi":"10.1016/j.bcab.2025.103544","DOIUrl":"10.1016/j.bcab.2025.103544","url":null,"abstract":"<div><div>According to traditional medicine, honey has been used as a natural wound-healing compound. However, honey exists in different forms based on its geographical existence and environmental factors, leading to its variation in properties. Thus, the present study aims to compare four different varieties of honey of Indian origin (acacia honey (AH), eucalyptus honey (EH), neem honey (NH), tulsi honey (TH)) with medical-grade manuka honey (MH) for their characteristics and wound healing potential. Overall, their secondary metabolite content was greater than 404.36 ± 16.02 mg GAE/g of total phenolic content and &gt;43.04 ± 0.82 mg QCE/g of total flavonoid content. Thereafter, the different honey samples were loaded into thermo-responsive hydrogels containing HPMC and Poloxamer 407 before these honey products were evaluated for their <em>in vitro</em> antioxidant, anti-inflammatory, antimicrobial, and <em>in ovo</em> angiogenic properties. <em>In vitro</em> cytotoxicity assay and wound closure evaluation depicted safety and efficacy in immortalized human keratinocyte (HaCaT) cell lines. Additionally, all the honey-loaded formulations exhibited no dermal irritation and corrosion in the <em>in vivo</em> model. Further, the honey-containing <em>in-situ</em> hydrogels were evaluated for their <em>in vivo</em> wound repair ability, biochemical investigations for <em>ex vivo</em> antioxidant effects, and ability to modulate extracellular matrix formation. Overall, it could be concluded that the Indian honey-loaded formulated hydrogels are safe for topical applications and efficacious in healing wounds.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103544"},"PeriodicalIF":3.4,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA interference-based gene silencing of dicer-like 2 in Fusarium oxysporum f. sp. cubense tropical race 4 mitigates fusarium wilt disease in banana","authors":"Egoda Ambhagahage Epitawaththe Samitha Sawindri Jayasekara ,&nbsp;Ganesan Vadamalai ,&nbsp;Norsazilawati Binti Saad ,&nbsp;Jin Hailing ,&nbsp;Wong Mui-Yun","doi":"10.1016/j.bcab.2025.103541","DOIUrl":"10.1016/j.bcab.2025.103541","url":null,"abstract":"<div><div>Banana Fusarium wilt, caused by <em>Fusarium oxysporum</em> f. sp. <em>cubense</em> tropical race 4 (<em>Foc</em> TR4) is a severe threat to global banana production which drastically reduces yields. Currently, no effective control measures exist for this destructive pathogen. RNA interference (RNAi)-based exogenous application of double-stranded RNAs (dsRNAs) has emerged as an environmental-friendly plant protection strategy in recent years. This innovative approach combats plant pathogens by silencing their essential genes. The dicer-like 2 (DCL2) gene which is an important component in the RNAi pathway, was silenced using <em>in vitro</em> synthesized long dsRNAs. The efficacy of <em>Foc</em> TR4-DCL2 dsRNAs was evaluated <em>in vitro</em> to determine optimal concentration and duration against <em>Foc</em> TR4, and their potential to suppress disease progression <em>in planta</em>. Relative transcript abundance analysis revealed that DCL2 gene was partially silenced at a threshold concentration of 1000 ng/mL after 72 h of dsRNA treatment, compared to the control (water) <em>in vitro</em>. <em>In planta</em> studies demonstrated that disease progression in roots and rhizomes was significantly inhibited in plants treated with <em>Foc</em> TR4-DCL dsRNAs, indicating that RNAi-mediated gene silencing mechanism could be a promising method for controlling banana Fusarium wilt in the future.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103541"},"PeriodicalIF":3.4,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diesel oil degradation and biosurfactant potential of novel indigenous bacterial strain Onchobactrum intermedium","authors":"Diksha Sah ,&nbsp;Pallavi Saxena ,&nbsp;Mika Sillanpaa ,&nbsp;Ashish Kapoor ,&nbsp;Ankita Ghosh ,&nbsp;Moumita Chakraborty ,&nbsp;Jai Prakash Narayan Rai","doi":"10.1016/j.bcab.2025.103535","DOIUrl":"10.1016/j.bcab.2025.103535","url":null,"abstract":"<div><div>The extensive worldwide usage of Petroleum Hydrocarbons (PHs) for energy leads to the petroleum spills posing significant hazards to environmental health. The remediation procedure for these pollutants is often extensive, laborious, and relies heavily on chemical treatments, which contradict the principles of sustainable development. Hence, the development of green technology-based solutions for remediation drives is imperative.</div><div>This study highlights the significance of utilizing <em>Onchobactrum intermedium</em> as an eco-friendly and cost-effective resource for environmental remediation, offering a sustainable and efficient approach to mitigating petroleum spill-related hazards. Interestingly, this bacterium was isolated from diesel oil-contaminated soil in the Haldwani &amp; Lalkuan region of Uttarakhand, India, and identified on the basis of 16S rRNA sequence analysis, embarking this study as a first report of the bacterium claiming as a diesel oil degrading agent.</div><div>Through gravimetric analysis, the study found that <em>Onchobactrum intermedium</em> breaks down 55.17% of diesel oil in 35 days at 30 °C. Remarkably, the treatment reduced the half-life of diesel from 76.5 days to 27.5 days compared to the control. GC-MS examination of the residual diesel showed degradation of organic components between C9 and C27, with C21 exhibiting substantial degradation competence.</div><div>Moreover, <em>Onchobactrum intermedium</em> was recognized as a potential fabricator of biosurfactants, with lipopeptides biosurfactant production documented at 2.85 g/L. These biosurfactants can improve the emulsification of diesel oil, making <em>Onchobactrum intermedium</em> more operative for diesel oil degradation. This study not only advances our understanding of microbial bioremediation but also provides a practical solution that aligns with sustainable development goals.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103535"},"PeriodicalIF":3.4,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143610683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of metabolic profile of various organs (root, stem, and leaf part) in Sida acuta Burm. f. and exploration of antioxidant and antifungal properties associated characteristic metabolic compounds","authors":"Lei Zeng,&nbsp;Yingle Chen,&nbsp;Liting Liang,&nbsp;Liu Yang,&nbsp;Song Wang,&nbsp;Qiaolin Xu,&nbsp;Zhihong Wang","doi":"10.1016/j.bcab.2025.103539","DOIUrl":"10.1016/j.bcab.2025.103539","url":null,"abstract":"<div><div><em>Sida acuta</em> Burm. f. (SA) is a traditionally important herb used to treat asthma, headaches, diabetes, and leucorrhoea. However, limited phytochemical and biological studies are available for the various organs of SA. Metabolomics, a fast-evolving technology, enables in-depth exploration of plant metabolic diversity and function. Therefore, the present study aimed to investigate the total polyphenol and flavonoid content, as well as specialized metabolite profiling of different organs of SA, and to discuss their relationships with biological activities (antioxidant and antifungal). The stem of SA exhibited the highest contents of total phenols (70.46 mg GAE/g DW) and total flavonoids (135.87 mg RE/g DW) among the analyzed plant organs. All extracts of SA demonstrated noteworthy antioxidant and antifungal potential. A total of 2117 metabolites were identified by UPLC-MS/MS. The stem was a source of phenolic acids, alkaloids, terpenoids, and tannins, with superior antioxidant capacities. Multivariate analyses unveiled a specific accumulation of metabolites in various organs of SA, with 446, 597, and 281 differentially accumulated metabolites, of which 42 metabolites were shared among the pairwise comparisons. KEGG enrichment analysis showed that flavone and flavonol biosynthesis, phenylpropanoid biosynthesis, flavonoid biosynthesis, galactose metabolism, and tryptophan metabolism were the most differentially regulated pathways. Furthermore, the Pearson correlation between antioxidant/antifungal activity and metabolites was found to be both positive and negative. A total of 107 potential metabolic biomarkers were positively associated with these bioactivities. This comprehensive investigation enhances our understanding of the metabolome and biological activity, supporting the health benefits of SA.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103539"},"PeriodicalIF":3.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of enzymatic and metabolic of a biocomposite based on Trichoderma koningiopsis and chlorella biomass","authors":"Simone Kubeneck ,&nbsp;Aline Frumi Camargo ,&nbsp;Vitória Dassoler Longo ,&nbsp;Larissa Capeletti Romani ,&nbsp;Júlia Pieper Nerling ,&nbsp;Suzana Fátima Bazoti ,&nbsp;Carlos Henrique Pagno ,&nbsp;Eliseu Rodrigues ,&nbsp;Helen Treichel","doi":"10.1016/j.bcab.2025.103542","DOIUrl":"10.1016/j.bcab.2025.103542","url":null,"abstract":"<div><div>The gradual concern with promoting sustainability has encouraged the search for new methodologies for obtaining bioproducts. This study, therefore, analyzed the generation of a fungal biocomposite using <em>Trichoderma koningiopsis</em> and biomass of the microalgae <em>Chlorella</em> sp. from swine wastewater, aiming at a product with potential for environmental and agronomic application. The process involved submerged fermentation in an Airlift bioreactor, using microalgal biomass as the substrate and <em>Trichoderma koningiopsis,</em> such as fermentative microorganisms. Afterward, the enzymatic activity and stability were evaluated, and the stability was monitored for 90 days at different storage temperatures (20 °C, 4 °C, and −80 °C). The results demonstrated considerable enzymatic activity of the biocomposite, mainly for catalase (1274,79 μmol/min.mL), peroxidase (52,08 U/mL), and protease (97,50 U/mL), with good stability over time. By analyzing the organic compounds before and after fermentation using UHPLC-ESI-qTOF-MS/MS, the production of metabolites such as tyrosine and leucine which have herbicidal potential, koningins a, b, and d, trichodermin, which are considered antifungal agents and of relevance in the health area, in addition to primary metabolites such as fructose, organic acids, and ethanol, was observed. Therefore, through this study, it was possible to highlight the potential of the biocomposite obtained as a matrix of products aimed at the environment and agricultural level through enzymes and metabolites of interest for producing bioinputs and removing contaminants in effluents.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103542"},"PeriodicalIF":3.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of potential anti-inflammatory activities in crude, clean, and fractionated extracts of rosmarinic acid and eupatorin from Orthosiphon aristatus (Blume) Miq","authors":"Salfarina Ezrina Mohmad Saberi ,&nbsp;Lee Suan Chua","doi":"10.1016/j.bcab.2025.103537","DOIUrl":"10.1016/j.bcab.2025.103537","url":null,"abstract":"<div><div><em>Orthosiphon aristatus</em> has been demonstrated to possess natural anti-inflammatory properties. This study aims to concentrate rosmarinic acid (RosA) and eupatorin (EUP) content in the plant extracts using different ethanol percentages and to evaluate anti-inflammatory potential of RosA and EUP extracts. Reflux extraction of <em>O. aristatus</em> was conducted using different ethanol concentrations ranging from 60 % to 100 %. The results found that 60 % ethanol produced the highest yield of crude extract, while the highest yields of clean and fraction extracts were from 90 % ethanol. The optimal yield of RosA was in 80 % ethanol crude extract, and 90 % ethanol clean and fraction extracts. However, the highest yield of EUP was obtained from 60 % ethanolic fraction extract, whereas 100 % ethanol was optimal for crude and clean extracts. A substantial amount of EUP in 60_Fr exhibited the most effective inhibition of lipoxygenase (LOX), with an inhibition concentration of 27.91 μg/mL. Conversely, sample 100_Cr characterized by a high concentration of RosA, demonstrated the highest inhibitory effect of α-amylase, with an inhibition value of 100.41 μg/mL. Ethanol concentration played an important role to concentrate both bioactive compounds in the plant extracts. Different ethanol concentrations would recover varying levels of compounds in the crude, clean and fraction extracts to relate to their changes in anti-inflammatory properties. Concentrating EUP enhanced the LOX inhibition, whereas concentrating RosA promoted α-amylase inhibition. The findings of this study are critical for identifying therapeutic compounds in different forms of <em>O. aristatus</em> extracts to treat inflammatory-induced illnesses.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103537"},"PeriodicalIF":3.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}