Biocatalysis and agricultural biotechnology最新文献

{"title":"Effect of green synthesized silver nanoparticles using the aqueous extract of Lavandula angustifolia Mill. Against ethanol-induced gastric ulcers in rats","authors":"Maryam Moradi ,&nbsp;Parichehr Hanachi ,&nbsp;Seifollah Bahramikia ,&nbsp;Mansoureh Tavan","doi":"10.1016/j.bcab.2025.103584","DOIUrl":"10.1016/j.bcab.2025.103584","url":null,"abstract":"<div><div><em>Lavandula angustifolia</em> Mill. (Lamiaceae) possess valuable bioactive compounds such as polyphenols and thus this plant is gaining interest to green synthesis of nanoparticles as source of reducing, stabilizing, and capping agents. ‏Therefore, in the present study, aqueous extract of.</div><div><em>L. angustifolia</em> was used for green synthesis of silver nanoparticles (La-AgNPs). The characterizations of La-AgNPs were determined by UV–Vis spectroscopy, FTIR, XRD, SEM, and EDX. Additionally, the effect of La-AgNPs on gastric damage caused by ethanol in desert rats was investigated. Firstly, Wistar rats were pre-treated with La-AgNPs (5 and 20 mg/kg) and famotidine at (50 mg/kg). Then, an oral dose of ethanol was administered to rats to induce gastric ulcers. The results showed that La-AgNPs had optimal size (69.35 nm) and spherical and cubical shapes. Moreover, the consumption of La-AgNPs orally caused a significant decrease in gastric ulcer index and gastric juice volume, as well as an increase in pH. In addition, tissue nitric oxide (NO), superoxide dismutase, catalase, and glutathione levels increased and serum NO, reactive oxygen species, and malondialdehyde levels decreased in rats pre-treated with La-AgNPs and famotidine drug compared to the ethanol group. The histopathological results confirmed the above cases. This study showed that La-AgNPs can be a promising treatment for gastric ulcer disease caused by ethanol consumption.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103584"},"PeriodicalIF":3.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable management strategy for converting poultry feather waste into valuable organic fertilizer: Improving the growth of Dalbergia sissoo using feather-based compost","authors":"Amira F.Y. El-Kady ,&nbsp;Nabila I. El-Sayed","doi":"10.1016/j.bcab.2025.103577","DOIUrl":"10.1016/j.bcab.2025.103577","url":null,"abstract":"<div><div>Sustainable agriculture faces challenges in managing agro-industrial biomass waste, particularly poultry feathers. Proper management of this naturally occurring, biodegradable raw organic waste can help improve soil fertility, promote plant growth, and reduce environmental stresses. This study was conducted at the Faculty of Agriculture, Cairo University, Egypt, to assess the efficacy of using various types of animal feces in the preparation of poultry feather-based composts, the quality of the prepared composts, and their potential effectiveness in improving the growth performance and chemical components of a medicinal leguminous tree (<em>Dalbergia sissoo</em> Roxb.) without compromising the environment. The results demonstrate that all feather-based composts enriched with animal feces had appropriate pH and C:N ratios, high concentrations of ash and NPK, and low concentrations of organic matter and total organic carbon when compared to feather compost without animal feces. Seedlings supplied with feces-enriched composts showed comparable growth and leaf content of photosynthetic pigments, total carbohydrates, amino acids, phenols, and flavonoids, as well as antioxidant activity, to those supplied with synthetic NPK fertilizers (control). The superior performance was obtained by compost prepared using fish culture sludge, followed by broiler chicken excreta, then the feces mixture, sheep dung, cow dung, and compost prepared without feces. Our study presents a sustainable nutrient recycling strategy based on the efficient and cost-effective conversion of poultry feather waste into an eco-friendly organic fertilizer (compost) for the fertilization of an economical medicinal tree species with broad ecological and landscape benefits in poor soils.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103577"},"PeriodicalIF":3.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimisation of diesel degradation and growth kinetic modelling by Antarctic Janthinobacterium lividum","authors":"How Swen Yap ,&nbsp;Farah Eryssa Khalid ,&nbsp;Rasidnie Razin Wong ,&nbsp;Peter Convey ,&nbsp;Suriana Sabri ,&nbsp;Khalilah Abdul Khalil ,&nbsp;Azham Zulkharnain ,&nbsp;Faradina Merican ,&nbsp;Hasrizal Shaari ,&nbsp;Siti Aqlima Ahmad","doi":"10.1016/j.bcab.2025.103573","DOIUrl":"10.1016/j.bcab.2025.103573","url":null,"abstract":"<div><div>Fuel spills pose significant pollution threats to Antarctic terrestrial environments. Biological remediation offers a sustainable solution for restoring these polluted sites. Indigenous microorganisms capable of degrading diesel hydrocarbons at low temperatures could have the potential to restore polluted sites in Antarctic regions. This study represents the first comprehensive investigation into the effects of physiochemical conditions on diesel biodegradation and the growth kinetics of <em>Janthinobacterium lividum</em>, a native Antarctic bacterium previously identified for its diesel-degrading and biosurfactant-producing capabilities at low temperatures. Using a one-factor-at-a-time (OFAT) approach followed by response surface methodology (RSM), we optimised key culture conditions (10.00 °C, 0.00 % NaCl, 1.49 g/L NH4Cl, pH 7.05, 5.67 % v/v initial diesel concentration, and 14.10 % v/v inoculum size), achieving a five-fold enhancement in total petroleum hydrocarbon degradation, reaching 20.57 mg/mL. The Aiba-Edwards growth kinetics model mathematically best described the growth kinetics of the species in the presence of a toxic substrate. This study demonstrates statistically guided optimisation strategies for enhancing diesel biodegradation in <em>Janthinobacterium</em> species and demonstrates the bacteria's remarkable ability to tolerate high diesel concentrations even at low temperatures, reinforcing their potential for Antarctic bioremediation applications.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103573"},"PeriodicalIF":3.4,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight into the potential of a novel activated carbon from rumen waste and its green application in tartrazine dye removal from effluents","authors":"Lucas Silva de Sousa ,&nbsp;Priscilla Amaral Nascimento ,&nbsp;Mateus Pereira Flores Santos ,&nbsp;Mylena Junqueira Pinto Brito ,&nbsp;Annie Nolasco Alves ,&nbsp;Felipe Rocha ,&nbsp;Cristiane Martins Veloso ,&nbsp;Renata Cristina Ferreira Bonomo","doi":"10.1016/j.bcab.2025.103571","DOIUrl":"10.1016/j.bcab.2025.103571","url":null,"abstract":"<div><div>This study aimed to use bovine rumen waste as a precursor for the synthesis of activated carbon, as well as to apply the produced carbon in effluent treatment for the removal of tartrazine dye. The bovine rumen waste exhibited suitable characteristics as a carbon precursor, including a high lignocellulosic content, and can be used to synthesize activated carbon. Phosphoric acid was used for chemical activation (105 °C for 24 h at a 1:1 ratio), and functional groups were effectively incorporated onto the adsorbent surface, resulting in acidic carbon. At a pH of 3, the adsorbent showed high efficiency in removing tartrazine dye, with the adsorption capacity influenced by temperature (111.90 mg.L⁻¹ at 318.15 K). The best-fitting isotherm model was Freundlich, and the adsorption was found to be endothermic, thermodynamically favorable at all studied temperatures. Therefore, activated carbon from bovine rumen waste has the potential to be a sustainable and low-cost adsorbent for removing dyes from industrial effluents.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103571"},"PeriodicalIF":3.4,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing arabica and canephora coffee cascara as substrates for kombucha: Comparative study of chemical, antioxidant, antibacterial and antifungal properties","authors":"Mariana Moysés Delorme ,&nbsp;Beatriz Spena Machado ,&nbsp;Fernanda dos Santos Ferreira ,&nbsp;Rayssa Neves Alves ,&nbsp;Daniel Coelho Ferreira ,&nbsp;Jessica Pronestino de Lima Moreira ,&nbsp;Vanessa Naciuk Castelo-Branco ,&nbsp;Thais Matsue Uekane ,&nbsp;Fabrício Freitas Fernandes ,&nbsp;Adriene Ribeiro Lima","doi":"10.1016/j.bcab.2025.103570","DOIUrl":"10.1016/j.bcab.2025.103570","url":null,"abstract":"<div><div>This study aimed to investigate kombuchas produced from <em>Coffea arabica</em> L. (KA) and <em>Coffea canephora</em> Pierre (KC) cascara, combined with black tea (<em>Camellia sinensis</em>), fermented over a 12-day period. The evaluation focused on their chemical properties, antioxidant potential, and antimicrobial activity. A decrease in pH and an increase in acidity were observed along the fermentation period. There was an increase in total phenolic content by 27.4 % in KA and 22.7 % in KC, however antioxidant activity, as determined by DPPH and FRAP assays, remained unchanged. The 5-caffeoylquinic acid (5-CQA) was the predominant phenolic acid identified, exhibiting higher concentrations in the fermented beverages compared to their respective non-fermented infusions, highlighting the impact of fermentation on its concentration. Additional phenolic acids identified included gallic, caffeic, p-coumaric, 3,4-dihydroxybenzoic, sinapic, and ferulic acids. KC exhibited higher levels of total phenolic content, 5-CQA, catechin, and caffeine compared to KA, as well as stronger antioxidant activity. Both beverages demonstrated antibacterial activity against tested strains from the 6th day of fermentation. KA exhibited bactericidal activity against <em>Salmonella</em> Enteritidis and fungicidal activity against <em>Candida albicans</em> and <em>Candida parapsilosis</em> on the 12th day, whereas KC showed no bactericidal or antifungal effects. As far as we know, this is the first study to report the antimicrobial properties of kombucha produced from coffee cascara. These findings suggest that arabica and canephora coffee cascara infusions are viable alternative substrates for kombucha production. The use in the development of fermented beverages with functional properties is a promising way of using by-products from coffee processing.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103570"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic selenium nanoparticles as nanopriming agents: Promoting germination and strengthening antioxidant defense in rice (Oryza sativa L.)","authors":"Monalisha Mishra,&nbsp;Nidhi Shukla,&nbsp;Mariyam Fatima,&nbsp;Nand K. Singh","doi":"10.1016/j.bcab.2025.103568","DOIUrl":"10.1016/j.bcab.2025.103568","url":null,"abstract":"<div><div>Biogenic synthesis of nanoparticles is an emerging and trending technology. Greener approaches are more sustainable, less invasive and economical. The contemporary study focuses on the synthesis of selenium nanoparticles (Se-NPs) using <em>Hibiscus rosa-sinensis</em> leaf extract and evaluating their nanopriming effect on rice seeds. The biophysical characteristics of Se-NPs were evaluated using techniques like ultraviolet–visible spectroscopy, fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction, dynamic light scattering, scanning electron microscopy (SEM), atomic force microscopy (AFM) and electron probe microanalysis (EPMA). SEM and AFM analyses revealed spherical Se-NPs with an average diameter of 47.6 nm. FT-IR confirmed functional groups, aiding in the reduction and stabilization of the Se-NPs, while EPMA validated elemental selenium presence. Nanopriming with Se-NPs demonstrated a significant, dose-dependent increase in germination activity. This was evident in mean germination time (65 %), radicle and plumule length, fresh weight, and biochemical assays for total soluble sugar and protein content. Additionally, seed vigour and relative water uptake measurements strongly indicated that Se-NPs effectively boost germination and seedling growth. The reduction of free radicals was observed by 3,3′-diaminobenzidine and nitro blue tetrazolium staining, indicating strong antioxidant activity by application of Se-NPs. The increased activities of enzymes superoxide dismutase (SOD ≥28 %), catalase (CAT ≥46 %), peroxidase (POD ≥49 %), along with marked reduction in lipid peroxidation (MDA ≥23 %) evidently demonstrated that Se-NPs enhanced antioxidant defense. These findings suggest that selenium nanoparticles have bioactive potential in agriculture, particularly in improving plant growth. This warrants further exploration for efficient agricultural applications and creating a more sustainable environment.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103568"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of geraniol by a solvent-tolerant Serratia marcescens without the formation of geraniol derivatives","authors":"Yongai Ma ,&nbsp;Di Liu ,&nbsp;Long Wang ,&nbsp;Yao Lu ,&nbsp;Taiping Fan ,&nbsp;Huaxiang Deng ,&nbsp;Yujie Cai","doi":"10.1016/j.bcab.2025.103567","DOIUrl":"10.1016/j.bcab.2025.103567","url":null,"abstract":"<div><div>Geraniol is an acyclic monoterpene compound with a distinct rose-like fragrance, extensively used in fragrances, cosmetics, pesticides, and pharmaceuticals. The traditional extraction of geraniol from plants is limited by the supply of raw materials, which is insufficient to meet market demands. The solvent-tolerant <em>Serratia marcescens</em> demonstrates a high potential for geraniol production, so it was selected as a host for the production of geraniol. We constructed a geraniol biosynthetic pathway in <em>S. marcescens</em> HBQA7 by integrating seven enzymes with high expression activity, successfully achieving the production of geraniol. To enhance the biosynthesis of isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP), we engineered the downstream pathway of the mevalonate (MVA) pathway by rearranging the order of phosphomevalonate kinase (PMK), phosphomevalonate decarboxylase (PMD), and isopentenyl diphosphate isomerase (IDI). Furthermore, an optimal geraniol synthase (ObGES) from <em>Ocimum basilicum</em> was determined for geraniol biosynthesis by evaluating five different geraniol synthases (GES). To further increase the production of geranyl pyrophosphate (GPP), we compared different geranyl diphosphate synthase (GPPS) and found that the enzyme derived from <em>Abies grandis</em> (AgGPPS) was the most effective. Based on these findings, we optimized the linker sequences for fusion expression, thereby improving geraniol production efficiency. Ultimately, the geraniol titer reached 947.6 mg/L in shake flask fermentation. Upon scaling up the process, the geraniol production further increased to 2976.2 mg/L in 5 L bioreactor. This represents the highest yield of geraniol to date in <em>S. marcescens</em>. This research provides a theoretical foundation for the production of other terpenoid compounds in <em>S. marcescens</em>.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103567"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of subcritical water extraction and precipitation methods on the functional properties, antioxidant activity, and ACE1 inhibition of palm kernel cake protein","authors":"Alfred Elikem Kwami Afedzi ,&nbsp;Thitirat Poolsawat ,&nbsp;Bandhita Wanikorn ,&nbsp;Pramuk Parakulsuksatid","doi":"10.1016/j.bcab.2025.103569","DOIUrl":"10.1016/j.bcab.2025.103569","url":null,"abstract":"<div><div>Protein is an essential macronutrient for human and animal nutrition. Plant protein sources can be classified into protein flours, protein concentrates, and protein isolates, each offering beneficial nutritional and functional properties. This study investigated the use of subcritical water (SCW) for extracting protein from palm kernel cake (PKC). SCW extraction was carried out at three temperatures (120, 150, and 180 °C) and two durations (15 and 30 min). These conditions were compared with a conventional alkali (2 M NaOH) extraction method. Two protein precipitation methods; isoelectric (pH 3.5) and cold ethanol (80 %), were investigated. Temperature and time significantly influenced protein yield. At 150 °C and 30 min, SCW extraction followed by cold ethanol precipitation resulted in the highest protein yield of 66.24 ± 4.22 %. Isoelectric precipitation under the same SCW condition gave only 1.33 ± 0.06 %. Conversely, the alkali method showed a higher protein yield (94.01 ± 3.99 %) via isoelectric precipitation. Cold ethanol precipitation with alkali extraction was less than 0.01 %. The protein content from SCW extraction was 42.01 ± 1.72 % (flour), while that from alkali extraction was 65.53 ± 1.15 % (concentrate). SCW protein flour showed higher antioxidant activity by DPPH (78.74 ± 2.33 μmol TE/g) and total phenolic content (190.43 ± 1.75 mg GAE/g). However, alkali protein concentrate had higher ABTS (87.88 ± 0.90 μmol TE/g) and reducing power (0.39 ± 0.05 μmol TE/g). SCW protein flour demonstrated the strongest ACE1 inhibition and favorable emulsifying, foaming, and absorption properties. These findings emphasize SCW extraction as an eco-friendly strategy for obtaining bioactive protein from PKC with potential applications in functional foods.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"66 ","pages":"Article 103569"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the industrial potential of Withania somnifera (L.) Dunal: A varietal comparison of morphology, microstructure, and metabolomic profiles","authors":"Sneha Singh,&nbsp;Pajeb Saha,&nbsp;Sabitri Kumari,&nbsp;Nidhi Rai,&nbsp;Shashi Pandey-Rai","doi":"10.1016/j.bcab.2025.103572","DOIUrl":"10.1016/j.bcab.2025.103572","url":null,"abstract":"<div><div>To address the lack of publications on the metabolic profiling of <em>W. somnifera</em> varieties for industrial and pharmaceutical uses, we conducted a comprehensive analysis of the phytochemistry in three ashwagandha varieties- Red (R), Wild (W), and Pratap (P)- with a focus on their distinctive traits and potential commercial applications. Our research involved studies on germination, morphological assessments, and tissue culture experiments to understand growth potential and physical traits. Additionally, microscopic examinations of leaf structures and advanced techniques such as Raman spectroscopy and high-resolution mass spectrometry were used to analyse their biochemical and metabolite profiles. R exhibited a 101.5 % increase in total biomass in the field, while W showed the most vigorous <em>in-vitro</em> growth. The P variety had a high density of glandular trichomes, crucial for secondary metabolite production. Raman analysis revealed Withaferin A was most abundant in R, and phenylalanine was highest in P. HRMS results indicated comparable levels of terpenes in W and P, with P having higher fatty acids and steroids, known for skincare benefits. Peptides and amines were similarly high in R and W, supporting their therapeutic uses. These comprehensive plans revealed notable differences in the chemical profiles of the three varieties, emphasizing their potential for various industrial applications. This study not only adds to the understanding of the phytochemical diversity within <em>W. somnifera</em> but also further enables the improvement of the efficiency of cultivation and processing techniques for better medicinal and commercial outcomes.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103572"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization, in vitro and in vivo biological activities of P. equisetiforme extract powder, and preformulation studies for hard gelatin capsules","authors":"Sourour Idoudi ,&nbsp;Walid Elfalleh ,&nbsp;Jalloul Bouajila ,&nbsp;Khaled Bellassoued ,&nbsp;Fatma Ben Mansour ,&nbsp;Mehrez Romdhane ,&nbsp;Audrey Tourrette","doi":"10.1016/j.bcab.2025.103565","DOIUrl":"10.1016/j.bcab.2025.103565","url":null,"abstract":"<div><div>The aim of this study was to evaluate the influence of two extraction solvents, ethanol (EtOH) and water (AQ), on the chemical composition (HPLC-DAD and GC-MS) and <em>in vitro</em> biological properties of <em>P. equisetiforme</em> aerial parts. Biological activities included antixanthine oxidase (anti-XOD), antiacetylcholinesterase (anti-AChE), anti-5-lipoxygenase (anti-5-LOX), anti-α-glucosidase, anti-α-amylase, as well as cytotoxic activity was evaluated. Subsequently, the <em>in vivo</em> hepatoprotective effect of the bioactive extract was also assessed. Finally, we made powder capsules with two different excipients, microcrystalline cellulose (MCC) and starch, and proceeded to characterize each formulation to optimize hard gelatin capsules. The results revealed that the ethanolic extract is more active than the aqueous extract, notably in DPPH (IC₅₀ = 6.52 μg/mL), ABTS (IC₅₀ = 3.13 μg/mL) tests, anti-AChE (IC₅₀ = 15.63 μg/mL), and antidiabetic activities via the α-glucosidase (IC₅₀ = 5.96 μg/mL) and α-amylase (IC₅₀ = 21.19 μg/mL) enzymes. This extract is also the most concentrated in polyphenols (427.72 GAE/g DW) and flavonoids (36.43 RE/g DW). For the <em>in vivo</em> part, results showed that pretreatment with <em>P. equisetiforme</em> EtOH extract significantly attenuated nickel (Ni)-induced elevated liver marker and lipid levels in rats. In addition, antioxidant enzyme activities and TBARS levels were significantly corrected by pretreatment with EtOH extract. The starch-based (F1) and MCC-based (F2) capsules maintained a considerable amount of bioactivity, despite the pure extract exhibiting greater anti-α-glucosidase and antioxidant properties. The polyphenol content and bioactive characteristics of the extract were retained by both formulations, according to stability tests conducted over a 30-day period. The results obtained support the use of starch and MCC as suitable excipients for the stable and efficient encapsulation of the ethanolic extract of <em>P. equisetiforme</em>.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103565"},"PeriodicalIF":3.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}