Biocatalysis and agricultural biotechnology最新文献

{"title":"Enhancing melatonin photostability and controlled release using pH-responsive mesoporous silica nanoparticles for agricultural applications","authors":"Parul Sangwan,&nbsp;Poonam Barala,&nbsp;Vinita Hooda","doi":"10.1016/j.bcab.2025.103497","DOIUrl":"10.1016/j.bcab.2025.103497","url":null,"abstract":"<div><div>pH-responsive and thiol-functionalized mesoporous silica nanoparticles (SH-MSNs) encapsulating the phytohormone melatonin were designed to release the hormone in a controlled manner while enhancing its photostability. Melatonin-loaded MSNs were prepared in weight ratios of 1:4, 1:2, 1:1, and 2:1, with the highest loading efficiency of 17.12 μg/mL observed at a 1:1 melatonin/SH-MSNs ratio. Detailed characterization of these MSNs confirmed their functionality: zeta potential and size analyses indicated enhanced stability post-functionalization and loading; FESEM confirmed spherical morphology; EDX and FTIR validated melatonin incorporation and disulfide linkage formation; and DSC and XRD analyses demonstrated the amorphous transformation of melatonin, enhancing dissolution rates. Reduced specific surface area and pore volume, as revealed by N₂ adsorption-desorption studies, confirmed melatonin entrapment. A distinct DSC peak at 118.82 °C reflected the crystalline nature of pure melatonin, contrasting with its amorphous state post-encapsulation. <em>In vitro</em> release studies demonstrated significant pH responsiveness, with 81 % melatonin release at pH 5.5 and 55% release at pH of 6.5 after 48 h due to disulfide bond reduction. Additionally, encapsulation improved melatonin photostability by 64.9% compared to free melatonin. This research suggests that MSNs grafted with redox-responsive gatekeepers could be used in crops to deliver melatonin in a controlled manner. Future research may explore the broader agricultural benefits of these MSNs for controlled release of other phytohormones under various environmental conditions.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103497"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143148753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spectral light treatment and exogenous feeding of precursors enhanced the picrosides content in dedifferentiated cell culture of Picrorhiza kurroa","authors":"Mahinder Partap ,&nbsp;Amit Kumar ,&nbsp;Pawan Kumar ,&nbsp;Dinesh Kumar ,&nbsp;Ashish R. Warghat","doi":"10.1016/j.bcab.2025.103501","DOIUrl":"10.1016/j.bcab.2025.103501","url":null,"abstract":"<div><div>The present investigation evaluates the impact of the light spectrum (red and blue) and precursors treatment on picrosides (P-I, P-II, P-III) and pathway precursors metabolites enrichment in the cell culture of <em>Picrorhiza kurroa</em>. Exogenously, vanillic acid (VA), phenylalanine (PAL), and trans-cinnamic acid (TCNA) precursors were used in 50–150 mg/L concentrations to leaf and rhizome cell suspension of <em>P. kurroa</em>. The maximum content of P-I (0.872 and 0.894 mg/g DW), P-II (0.892 and 0.837 mg/g DW), P-III (0.286 and 0.288 mg/g DW) were quantified in leaf and rhizome cell suspension cultures under red light treatment. The maximum content (mg/g DW) of picrosides (P-I; 9.881, P-II; 3.939, and P-III; 0.884), VA (0.236), and caffeic acid (CFA; 0.20) were quantified in 150 mg/L VA in leaf suspension culture. The maximum content of catalpol (CTP; 5.845 mg/g DW) was quantified in PAL 100 mg/L, while cinnamic acid (CAN; 0.176, 0.179, 0.162 mg/g DW) was quantified in 150 mg/L of VA, PAL, and TCNA. However, in rhizome suspension culture, the maximum content (mg/g DW) of P-I (7.881), P-III (0.964), and VA (0.324) were found in VA 150 mg/L. The results concluded that VA (100–150 mg/L) is most suitable for picrosides enrichment in the cell culture of <em>P. kurroa</em>. The study confirms that red light and vanillic acid (VA) treatment significantly enhanced the picrosides and precursor metabolite in cell cultures. By utilizing the current approach coupled with elicitor treatments and scale-up studies at bioreactor level could facilitate the large-scale production of picrosides.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103501"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143148754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A DNase from halophilic bacterium Bacillus pacificus targets two notorious biofilms of Pseudomonas aeruginosa PAO1 and Staphylococcus aureus","authors":"Sadaf Salim ,&nbsp;Vusqa Jadoon ,&nbsp;Mugheesa Batool ,&nbsp;Irfan Ali ,&nbsp;Usman Irshad ,&nbsp;Raza Ahmed ,&nbsp;Sadaf Qayyum ,&nbsp;Awal Noor ,&nbsp;Tatheer Alam Naqvi","doi":"10.1016/j.bcab.2025.103515","DOIUrl":"10.1016/j.bcab.2025.103515","url":null,"abstract":"<div><div>Biofilm is a sessile microbial community in which microbes are encased in a self-produced matrix called extracellular matrix (EPS), composed of eDNA, proteins and carbohydrates. Microbes in biofilms are several times more resistant to antimicrobial agents and host defense systems, thus are responsible for 60–80 % of human bacterial infections. Therefore, the enzymes that target EPS components can play a vital role against these biofilms. In the present study, an enzyme was isolated from halophilic bacterium <em>Bacillus pacificus</em> strain ROC1 and its antibiofilm potential was checked against the biofilms of <em>Pseudomonas aeruginosa</em> and <em>Staphylococcus aureus</em>. DNase assay confirmed that the enzyme is DNase, which was further confirmed by whole genome sequence analysis that identified the potential <em>DNase</em> gene in ROC1. For the production of DNase Luria Bertani (LB) media was employed and harvested after 24 h of growth. The disruption and inhibition assays confirmed the antibiofilm potential of DNase by inhibiting 80% biofilm biomass of <em>P. aeruginosa</em> and 71% biofilm biomass of <em>S. aureus</em>. It also disrupts the preformed biofilms of both strains, while more disruption was observed in <em>P. aeruginosa</em> (71%) as compared to <em>S. aureus</em> (55 %). Characterization of DNase indicates its robust nature with profound ability to catalyze in wide range of temperature and salt, and the addition of cations effects the enzyme activity.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103515"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143204539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing starch-stabilized biogenic silver nanoparticles for sustainable bacterial blight management in soybean","authors":"Federico N. Spagnoletti ,&nbsp;Irma N. Torres ,&nbsp;Alejandra I. Hernandez ,&nbsp;Romina Giacometti","doi":"10.1016/j.bcab.2025.103509","DOIUrl":"10.1016/j.bcab.2025.103509","url":null,"abstract":"<div><div>Green nanotechnology is a new strategy that contributes to sustainable agriculture by enhancing crop production, restoring soil quality, and improving pest management control. Green nanotechnology has emerged as a promising strategy to overcome the limitations of conventional chemical synthesis of nanomaterials. In this study, biogenic silver nanoparticles (st-AgNPs) were synthesized in the presence of extracellular metabolites of the fungus <em>Macrophomina phaseolina</em> with further modification of the particle's corona using starch to enhance stability and performance. After confirming the biocidal activity of st-AgNPs, their potential use against bacterial blight caused by <em>Pseudomonas savastanoi</em> in soybean was analyzed. Under controlled conditions, the effects of different NP concentrations were tested on healthy and infected plants. No evidence of phytotoxicity was observed in plants treated with 50–400 μg ml⁻¹ of NPs. A 100 μg ml⁻¹ st-AgNPs dose was the most effective in controlling the disease progression. Subsequent greenhouse experiments showed that infected plants sprayed with st-AgNPs (100 μg ml⁻¹) improved their general status and increased the aerial biomass (36.6%) compared to non-treated plants. Furthermore, spray treatment with NPs partially reversed the negative effect of the infection, showing a 38.9% recovery in the greenness index. Notably, the severity of infection decreased by 78%, with no detectable silver traces in plant leaves. Biochemical analyses revealed that st-AgNPs treatment resulted in a reduction of phenylalanine ammonia-lyase (PAL) and lipoxygenase (LOX) activities, as well as lipid peroxidation, compared to non-treated infected plants. These findings suggest that the developed st-AgNPs not only effectively control <em>P. savastanoi</em> infection in soybean but also exhibit the potential to serve as a low-impact, environmentally friendly tool for inclusion in plant disease management protocols across various crops.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103509"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trichoderma asperellum (T42)-mediated expression of CabHLH genes enhances nitrogen use efficiency and nutritional values of chickpea under salt and Fusarium wilt stresses","authors":"Nidhi Rai ,&nbsp;Shashi Pandey Rai ,&nbsp;Birinchi Kumar Sarma","doi":"10.1016/j.bcab.2025.103507","DOIUrl":"10.1016/j.bcab.2025.103507","url":null,"abstract":"<div><div><em>Trichoderma asperellum</em> T42 combats diverse phytopathogens and other stresses. However, its impact under combined stresses in modulating nutritional value and antioxidant properties in edible plant parts has not been thoroughly studied. Three chickpea cultivars, viz., wilt-resistant (JG-315), wilt-tolerant (JG-36), and wilt-susceptible (JG-62), were used to assess nutritional value and antioxidant contents under salt and pathogen (<em>Foc</em>)-challenged conditions. A sharp decrease in nodule numbers and biomass was observed in plants challenged with the combined stresses of <em>Foc</em> and salt in all three cultivars. However, seed treatment with T42 restored the nutritional value, enhanced antioxidant activities (1–2 folds) and increased total phenolic content (1.3–1.5 folds), protein (19–28%), proline, and micronutrients (7–28%) in chickpea seeds, particularly in the T42-treated plants subjected to the combined stress compared to the plants subjected to the combined stress without T42. The expression of two chickpea bHLH transcription factor genes, <em>CabHLH114</em> and <em>CabHLH115</em>, associated with nodule development and nitrogen fixation, varied under different stresses. The genes were upregulated in T42-treated plants and correlated with the development of root nodules. The results thus suggest that <em>Trichoderma</em>-mediated expression of both nodulation-responsive genes led to the formation of healthy and functional nodules, which helped improve nitrogen use efficiency in the chickpea plants and contributed to the nutritional value of the chickpea seeds. The results highlighted that reduction in nutritional value due to environmental stresses could be restored in crop plants by applying potential bioagents such as T42 that restore nutritional quality and make the crops climate resilient.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103507"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the wound healing potential of biocompatible nano-hydroxyapatite derived from parrotfish scale (Scarrus ghobban) waste for bone tissue engineering","authors":"Surya Parthasarathy ,&nbsp;Palanisamy Arulselvan ,&nbsp;Radha Gosala ,&nbsp;Balakumar Subramanian","doi":"10.1016/j.bcab.2025.103493","DOIUrl":"10.1016/j.bcab.2025.103493","url":null,"abstract":"<div><div>Fish waste management and the development of sustainable applications for fish byproducts have gained significant importance in recent times. This study focuses on repurposing discarded fish scales from <em>Scarus ghobban</em> to synthesize nano-hydroxyapatite (n-HAp) using an alkaline hydrolysis method. The n-HAp was comprehensively characterized through various analytical techniques, including FESEM, FT-IR, XRD, and TEM. The results revealed the successful production of n-HAp particles with an average size of 20–50 nm and the presence of functional groups such as esters, ethers, halogen compounds, and nitriles along with secondary amines, phenols, and alcohols which contribute to enhanced material characteristics. Additionally, through elemental mapping minerals such as Calcium, Phosphorous, Magnesium, and Sodium were confirmed. Cytotoxicity tests using L929 fibroblast cells demonstrated biocompatibility and wound healing assays indicated its potential for tissue regeneration. This approach not only harnesses valuable resources from fish waste but also holds promise for various biomedical applications, contributing to both economic growth and environmental sustainability. Ultimately, this nano-hydroxyapatite derived from fish scales showcases remarkable biocompatibility, positioning it as a promising candidate for the development of wound dressings.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103493"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological valorization of yeast strains for lipids and carotenoids production from renewable resources","authors":"Andreísa Teixeira de Castro ,&nbsp;Angélica Cristina de Souza ,&nbsp;Cristina Ferreira Silva ,&nbsp;Silvia Juliana Martinez ,&nbsp;Rosane Freitas Schwan ,&nbsp;Disney Ribeiro Dias","doi":"10.1016/j.bcab.2025.103499","DOIUrl":"10.1016/j.bcab.2025.103499","url":null,"abstract":"<div><div>This study aimed to select yeast strains from the Agricultural Microbiology Culture Collection (CCMA) to produce lipids and/or carotenoids using crude glycerol and sugarcane molasses as alternative carbon sources. Among the selected strains, nine exhibited oleaginous characteristics, with <em>Torulaspora maleeae</em> (CCMA 0039) reaching the highest accumulation of lipid content (77.7%) in the glucose medium. Notably, the <em>Exophiala spinifera</em> (CCMA, 2073) strain exhibited 35% lipid accumulation in glycerol pure. Furthermore, <em>Rhodotorula dairenensis</em> (CCMA 945), <em>Rhodotorula mucilaginosa</em> (CCMA 0156), <em>Rhodosporidium toruloides</em> (CCMA, 2032) and <em>Cystofilobasidium ferigula</em> (CCMA 1623) were identified as carotenogenic. Crude glycerol has proven to be the most effective medium for lipogenesis and carotenogenesis, also expressing diversity in fatty acid profiles, with <em>C. ferigula</em> demonstrating promise in the coproduction of lipids and carotenoids, achieving 41.88% lipid accumulation and 2.76 μg/mL of total carotenoids. These findings highlight the potential for utilizing industrial by-products for the sustainable production of important metabolites, paving the way for their integration into industrial bioprocesses.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103499"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging compost extract for heavy metal bioremediation and preserving the antioxidant activity of celery cabbage: A sustainable approach","authors":"Mohammed Haroun ,&nbsp;Ali Barham ,&nbsp;Juanjuan Wang ,&nbsp;Xiaoqing Qian","doi":"10.1016/j.bcab.2024.103483","DOIUrl":"10.1016/j.bcab.2024.103483","url":null,"abstract":"<div><div>The study examined the efficiency of a combined bioadditive containing molasses with <em>Bacillus firmus</em> and <em>Pseudomonas aeruginosa</em> in the production of compost extract and their impact on the physiochemical, heavy metals bioremediation, and antioxidant activity in the plant. The methods involved aeration and a compost-to-water ratio of 1:10, following the CRD. The extract physiochemical parameters were measured. The extract heavy metals were determined according to ICP-MS (inductively coupled plasma-mass spectrometry). The activities of catalase, peroxidase, ascorbate peroxidase, and superoxide dismutase were determined using the methods of liquid portion. The results showed significant improvements in the pH from 5.5 to 8, Ec from 7.1 to 8.2, and O.M from 27.5% to 50%. The TN increased from 0.14 to 0.203 mg/kg, TP from 0.46 to 1.24 mg/kg, TK from 0.25 to 0.58 mg/kg, Ca from 20.3 to 25.11 mg/kg, and Mg from 10.75 to 7.75 mg/kg. Besides, the heavy metal bioremediation; Fe was lightly reduced from 8.3 to 7.4 mg/kg, Zn from 1.0 to 0.1 mg/kg, Cu from 0.1 to 0.01 mg/kg, and Cd from 0.1 to 0.02 mg/kg. CE significantly improved plant shoot and dry weight. The antioxidant activity of SOD and POD was significantly higher than APX and CAT. A positive correlation between physiochemical properties and heavy metals, while a negative correlation with antioxidant activity, specifically APX and POD. Beneficially, the application of CE enhances antioxidant enzyme activities, reduces heavy metal uptake in plants, and adopts healthier growth, thereby supporting sustainable agricultural practices.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103483"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143148748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological, ultrastructural, biochemical and yield attributes variations in Nigella sativa L. by cadmium (Cd) stress: Implications for human health risk assessment","authors":"Adnan Khan ,&nbsp;Athar Ali Khan ,&nbsp;Sayma Samreen ,&nbsp;Moh Sajid Ansari ,&nbsp;Syed Aiman Hasan ,&nbsp;Mohd Irfan","doi":"10.1016/j.bcab.2025.103514","DOIUrl":"10.1016/j.bcab.2025.103514","url":null,"abstract":"<div><div>This study was conducted in the pots where each pot was filled with 4 kg soil that was contaminated with varying concentrations of cadmium (Cd) which are 1.5 mM, 2.0 mM, 2.5 mM, 3.0 mM, 3.5 mM, and 4.0 mM equal to 168.61, 224.82, 281, 337.23, 393.44 and 449.64 mg/L respectively), except the control group, each treatment was replicated thrice (n = 3) including control. We evaluated and examined growth, yield, metal stress tolerance, and metal buildup characteristics at the harvesting stage of black cumin (Nigella sativa L.) plants. The findings indicated that all levels of Cd had a substantial (p ≤ 0.05) negative impact on black cumin plants' growth and yield-related characteristics. Low (1.5 mM) to high doses (4.0 mM) of Cd significantly (<em>p</em> ≤ 0.05) reduced chlorophyll <em>a</em> (30%–72%), chlorophyll <em>b</em> (25%–69%) and total chlorophyll (56% to 82%) levels in the fresh leaf tissues, as well as significantly (<em>p</em> ≤ 0.05) decreased the fruit yield (12%–45%) and seed yield (39%–75%) of black cumin plants. Energy dispersive x-ray (EDX) analysis and scanning electron microscopy (SEM) of control and Cd-treated plant leaf samples showed significant Cd accumulation, and the ultrastructure of both revealed that Cd stress reduced the stomatal density and size of the stomatal aperture compared to the control. Bioaccumulation levels of Cd in the seeds of all treated plants are above the threshold limit (0.3 mg/kg) set by the World Health Organization (WHO) for medicinal plants and are unsafe for human consumption. Therefore, farmers must avoid cultivating black cumin crops in Cd polluted soil from economic and health perspectives.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103514"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143369765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms underlying the decolorization of indigo carmine and coomassie blue R-250 by Streptomyces salinarius CS29 laccase","authors":"Kamonpan Sanachai ,&nbsp;Bodee Nutho ,&nbsp;Rakrudee Sarnthima ,&nbsp;Wiyada Mongkolthanaruk ,&nbsp;Jirada Pluemjai ,&nbsp;Methus Kittika ,&nbsp;Saranyu Khammuang","doi":"10.1016/j.bcab.2025.103513","DOIUrl":"10.1016/j.bcab.2025.103513","url":null,"abstract":"<div><div>Laccase, a multicopper oxidase enzyme, possesses broad substrate specificity, enabling the oxidation of a diverse array of compounds. Among various microbial sources, <em>Streptomyces</em> species are prominent for producing stable and highly efficient laccases. This study investigated the decolorization potential of crude laccase extracted from <em>Streptomyces salinarius</em> CS29, specifically targeting indigo carmine and Coomassie Blue R-250 (CBBR). Optimal decolorization of both dyes was achieved within a pH range of 3–3.5, with pH 3.5 selected for subsequent experiments. Indigo carmine, at a concentration of 100 μM, demonstrated superior decolorization efficiency, reaching approximately 90% within 100 min. In contrast, decolorization of CBBR was less efficient. At concentrations of 50, 100, and 250 μM, approximately 50% decolorization was observed after 180 min. These findings suggest that laccase from <em>S. salinarius</em> CS29 exhibits greater efficacy in decolorizing indigo carmine compared to CBBR. Additionally, molecular docking and molecular dynamics (MD) simulations were employed to investigate the structural dynamics of the enzyme-dye complexes. MD simulations revealed that both indigo carmine and CBBR bind within the active site of the enzyme, predominantly through van der Waals interactions. Furthermore, key binding residues crucial for these interactions were identified. The findings of this study offer a foundational understanding that could significantly contribute to the development of environmentally sustainable strategies for the detoxification of dye-contaminated wastewater.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103513"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143204541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}