Biocatalysis and agricultural biotechnology最新文献

{"title":"Development of method and analysis protocol for fatty acids derivatives of castor oil by using gas chromatography-mass spectrometry","authors":"Amzad Hossain,&nbsp;Akansha Pal,&nbsp;Shadeed Salem","doi":"10.1016/j.bcab.2024.103408","DOIUrl":"10.1016/j.bcab.2024.103408","url":null,"abstract":"<div><div>Castor oil is a vegetable oil extracted from castor beans, known for its diverse applications across the chemical industry, food industry, skincare products, biodiesel components, and pharmaceuticals. Analyzing the fatty acid composition of castor oil using Gas Chromatography-Mass Spectrometry (GC-MS) can be complex due to various factors inherent to fatty acids and the oil's composition. Therefore, the goal of this study is to effectively separate and analyze the major fatty acids present in castor oil using GC-MS. Castor oil was methylated using alcoholic potassium hydroxide, then neutralized with salicylic acid and centrifuged. The supernatant was diluted and injected into the GC-MS with a developed temperature program. The results indicated that castor oil contains five fatty acids: ricinoleic acid methyl ester, oleic acid methyl ester, linoleic acid methyl ester, stearic acid methyl ester, and palmitic acid methyl ester, with respective concentrations of 87.63%, 1.81%, 6.57%, 2.93%, and 1.07%. Among these, methyl ricinoleate was the most abundant at approximately 87.36%, while palmitic acid methyl ester had the lowest concentration at 1.07%. The order of fatty acids detected was methyl ricinoleate &gt; linoleic acid &gt; stearic acid &gt; oleic acid methyl ester &gt; palmitic acid. To our knowledge, this is the first report of an easy and efficient method for the analysis of castor oil. In conclusion, the developed methylation of castor oil and gas chromatography temperature program are suitable for the routine analysis of castor oil.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142532524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of a collagenase-producing bacterium from the soil contaminated with slaughterhouse waste","authors":"Safoora Majidi Ghahfarokhi,&nbsp;Fakhrisadat Hosseini,&nbsp;Parinaz Ghadam,&nbsp;Farzaneh Barati","doi":"10.1016/j.bcab.2024.103407","DOIUrl":"10.1016/j.bcab.2024.103407","url":null,"abstract":"<div><div>Microbial enzymes have been considered extensively due to their low cost as well as suitable production and stability. Collagenases are proteolytic biocatalysts that have various applications in medicine, industry, and research. In this study, gelatinolytic bacteria were isolated from the soil contaminated with slaughterhouse waste. The isolate with the highest gelatin degradation activity was chosen and characterized with 16 S ribosomal DNA (rDNA) sequence via polymerase chain reaction (PCR) method. The selected isolate was tested for biochemical characteristics. Thereafter, the collagenase production in an industrial medium containing bone powder was investigated for 30 h with the selected bacterium. The enzyme molecular weight was determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method, with the enzyme effect on the bone powder morphologically studied via scanning electron microscopy (SEM) analysis. The quantitative ninhydrin method and the qualitative zymography method were applied to measure the enzyme activity. The results indicated that enzyme production by the selected isolate reached its highest level after 25 h of cultivation. The enzyme with about 60 kDa molecular weight could efficiently degrade the collagen of bone powder.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An innovative formulation to improve spent coffee characteristics as soil fertilizer: Nutrient, chemical characterization and effects on plant germination","authors":"Simone Angeloni ,&nbsp;Giovanni Caprioli ,&nbsp;Marco Cespi ,&nbsp;Laura Acquaticci ,&nbsp;Ahmed M. Mustafa ,&nbsp;Agnese Santanatoglia ,&nbsp;Aldo D'Alessandro ,&nbsp;Martina Coletta ,&nbsp;Natasha Waris ,&nbsp;Antonietta La Terza","doi":"10.1016/j.bcab.2024.103394","DOIUrl":"10.1016/j.bcab.2024.103394","url":null,"abstract":"<div><div>Spent coffee grounds (SCG) possess high organic content and various essential nutrients. However, their direct use as a fertilizer is limited due to high acidity, phenolic content, and C/N ratio. Therefore, in this study, SCG-based fertilizer (SCG_f) was developed by mixing the raw SCG (SCG_r) and zein through a granulation process and was compared with the SCG_r. According to the obtained results, in SCG_f, a reduction in the C/N ratio from 22.9 to 10.9, improved flowability from fair to good, an increase in the concentration of elements (N, C, S), nutrients (ash, fats, proteins), macrominerals (Ca, Mg, P, K, Na), microminerals (Cu, Fe, Mn, Zn), total polyphenolic content (TPC), total flavonoid content (TFC), and antioxidant activities (AOA) was noted. Additionally, a decrease in total organic acids, total chlorogenic acids, and was also observed in SCG_f. Regarding phytotoxicity test results obtained by using <em>Sinapis alba</em>, <em>Sorghum saccharatum</em>, and <em>Lepidium sativum</em> and treating them with 2.5%, 5%, and 10% of SCG_f and SCG_r, better germination was observed for SCG_f in <em>S. alba</em> (5%, 10%), <em>L. sativum</em> (2.5%, 5%). However, in <em>L. sativum</em>, 5% of SCG_r and 10% of both SCG_r and SCG_f completely inhibited seed germination. For <em>S. saccharatum</em>, higher germination rates were observed for SCG_f (2.5%, 10%), and at 5%, a comparable effect of both SCG_f and SCG_r was noted. SCG_f gave the longest root length at all concentrations in all plants except for 2.5% in <em>S. alba</em>. In conclusion, this study addresses the effective use of SCG for sustainable agriculture while mitigating environmental impacts.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary 7,10-dihydroxy-8(E)-octadecenoic acid reduces fat accumulation and prevents hyperglycemia and hyperlipidemia in diabetic/obese KK-Ay mice","authors":"Fumiaki Beppu ,&nbsp;Shota Fuji ,&nbsp;Hak-Ryul Kim ,&nbsp;Ching T. Hou ,&nbsp;Kazuo Miyashita ,&nbsp;Masashi Hosokawa","doi":"10.1016/j.bcab.2024.103390","DOIUrl":"10.1016/j.bcab.2024.103390","url":null,"abstract":"<div><div>7,10-Dihydroxy-8(<em>E</em>)-octadecenoic acid (DOD) is a dihydroxy fatty acid produced by <em>Pseudomonas aeruginosa</em> PR3 from oleic acid. Present study investigated preventive effects of dietary DOD on obesity, hyperlipidemia and hyperglycemia in diabetic/obese KK-<em>A</em>^<em>y</em> mice. After one week of acclimation, mice (5-week-old) were divided three groups fed with AIN-93G-based high fat diet (20% fat, w/w) as follows; Control group: 3% soybean oil + 17% lard, DOD 1% group: 3% soybean oil + 16% lard +1% DOD, DOD 2% group: 3% soybean oil + 15% lard + 2% DOD. Four weeks feeding with 1% and 2% DOD diets suppressed body weight gain and visceral fat accumulation, and improved blood glucose and serum lipids levels as well as hepatic lipid accumulation compared to control group. RT-PCR analysis revealed no changes were observed in mRNA of adiponectin (Adipoq) and proinflammatory TNF-α (Tnfa) and MCP-1 (Ccl2), but a decreasing trend in leptin mRNA expression in mesenteric white adipose tissue (WAT), suggesting that suppressed fat accumulation by DOD contribute to prevention of over production of leptin. We also observed a reduction of mRNA expression of hepatic insulin-target gluconeogenic enzymes such as PEPCK and G6Pase and the transcription factor sterol regulatory element-binding protein-1. These results showed that dietary DOD is effective to improve glucose and lipid metabolism in KK-<em>A</em>^<em>y</em> mice. The present study provides new insights showing potential use of dihydroxy fatty acids as functional lipids.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical composition of γ-irradiated German chamomile (Matricaria recutita L.) flower essential oils, acetylcholinesterase inhibitory activity and effects on growth inhibition of Staphylococcus aureus and Candida albicans","authors":"Yatish Pant ,&nbsp;Shubham Srivastava ,&nbsp;R.K. Lal ,&nbsp;Anand Mishra ,&nbsp;Laldingngheti Bawitlung ,&nbsp;Divya Bhatt ,&nbsp;Pankhuri Gupta ,&nbsp;Sakshi Yadav ,&nbsp;D.U. Bawankule ,&nbsp;Vidushi Tyagi ,&nbsp;Anirban Pal ,&nbsp;C.S. Chanotiya","doi":"10.1016/j.bcab.2024.103391","DOIUrl":"10.1016/j.bcab.2024.103391","url":null,"abstract":"<div><div>Radiation-induced mutations have been the most commonly used technique for the development of mutant varieties. The primary aim has been to upgrade the existing plant varieties by varying a few major traits responsible for limiting metabolite productivity. The increasing regulations on protected plant varieties and germplasms have restricted their use. The induced mutants have played a vital role in strengthening the plant breeding program. The medicinal and aromatic plants have been poorly explored for mutation breeding. CSIR-CIMAP has initiated a mutation breeding program on some of the high-value plants, like <em>Matricaria</em>. As a result, we could develop gamma-rays-induced stable accessions. Genus <em>Matricaria</em> is well known for the biosynthesis of diverse bioactive plant secondary metabolites as well as for its traditional and healthcare benefits documented in Ph. Eur. In the present work, gamma-irradiated stable accessions of German chamomile, <em>Matricaria recutita</em> L., have been investigated for their essential oil composition and biological assessment. After a thorough investigation for a two-year cropping period, we have observed that some accessions contain the highest α-bisabolol oxide B proportions (24.3–55.3%), while quite a few showed chamazulene (2.9–5.8%), α-bisabolone oxide A (6.5–38%), and α-bisabolol oxide A (30.1–55.3%). A few accessions were marked by the presence of lavandulol, an irregular monoterpenoid of high fragrance value. The antibacterial, anti-inflammatory, and acetylcholinesterase (AChE) inhibitory properties of these gamma-irradiated accessions were also evaluated. The accessions G6-R5-5 and G2-R1-6 exhibited maximum inhibition against <em>S. aureus</em> whereas G2-R1-1 arrested the growth of <em>C. albicans</em>. Furthermore, G2R1-3 composition is responsible for reducing AChE and inflammation in cell lines. These results may open up ways to develop plant-derived consumer products from <em>Matricaria recutita</em> L.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From millets to medicine: ADMET insights into diabetes management with P. sumatrense compounds","authors":"Komal G. Lakhani ,&nbsp;Poojaben Prajapati ,&nbsp;Rasmieh Hamid ,&nbsp;Saumya K. Patel ,&nbsp;Aditi Dwivedi ,&nbsp;Kirankumar P. Suthar","doi":"10.1016/j.bcab.2024.103396","DOIUrl":"10.1016/j.bcab.2024.103396","url":null,"abstract":"<div><div>Diabetes mellitus, a prevalent metabolic disorder characterised by impaired blood glucose regulation, has escalated into a global health crisis, exacerbated by high-glycaemic diets and unhealthy eating habits. While therapeutic agents such as acarbose mitigate diabetes by inhibiting alpha-amylase and alpha-glucosidase, their use is marred by adverse side effects, underscoring the need for safer, plant-based alternatives. This study explores the antidiabetic potential of Little Millet, an underutilised cereal grain with a low glycaemic index, rich in bioactive compounds. Unlike common staples such as wheat and rice, Little Millet contains unique compounds, including octodrine, 18,19-secoyohimban-19-oic acid, and phenylephrine, which were investigated for their novel application in diabetes management. Gas chromatography-mass spectrometry (GCMS) analysis identified 120 bioactive compounds, selected for their abundance and potential bioactivity. These compounds span fatty acids, phenols, flavonoids, organic acids, sugar alcohols, and amino acids, all of which were rigorously docked to alpha-amylase and human maltase-glucoamylase receptors. Notably, key ligands such as chlorogenic acid and 18,19-secoyohimban-19-oic acid exhibited superior binding affinities compared to acarbose, with promising drug-likeness and ADMET profiles, suggesting higher absorption and fewer side effects. A 100-ns dynamics simulation further validated the stability of these compounds, positioning them as viable candidates for diabetes treatment. These findings advance drug design and highlight the potential of incorporating Little Millet into diets for diabetes prevention. Further validation through <em>In-vitro</em>, <em>In-vivo</em>, and clinical studies is essential to fully realise their therapeutic potential. This research also opens avenues for exploring Little Millet in managing related conditions and enhancing its health benefits through breeding programmes.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Capparis spinosa: A rich source of phenolic compounds - A comprehensive review of its phytochemistry, health benefits, and biotechnological applications","authors":"Marouane Mohaddab ,&nbsp;Manon Genva ,&nbsp;Malika Fakiri ,&nbsp;Younes El-Goumi ,&nbsp;Abdelmonim Zeroual ,&nbsp;Marie-Laure Fauconnier","doi":"10.1016/j.bcab.2024.103409","DOIUrl":"10.1016/j.bcab.2024.103409","url":null,"abstract":"<div><div>Caper (<em>Capparis spinosa</em>) is a plant of significant socio-economic importance in the Mediterranean region which is traditionally used to fight various metabolic diseases. It has indeed long been recognized for its medicinal properties, notably attributed to its rich nutritional content and potent antioxidant activity due to phenolic compounds and other secondary metabolites. However, the commercial production of relevant secondary metabolites requires innovative approaches improving the biosynthesis of these metabolites to fulfill the industrial demand and mitigate the overexploitation of natural resources. For this end, different techniques such as cell suspension culture, hairy roots culture, biotic and abiotic elicitors supply as well as genetic engineering could be used to enhance the production of caper-derived secondary metabolites. In addition, omics tools including genomics, transcriptomics, metabolomics and proteomics can help to elucidate the biosynthetic pathway and altering the production of targeted metabolites. While this review first highlights the phytochemistry, ethno-pharmacological uses, and biological activities of caper, it also discusses the significance of <em>in vitro</em> culture systems, omics tools and metabolic engineering approaches to improve the production of caper-derived bioactive compounds.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142532523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing Bacillus safensis as biofertilizer for sustainable drought alleviation in Brassica juncea L","authors":"Nizakat Bibi ,&nbsp;Mohsin Khan ,&nbsp;Fazal ur Rehman ,&nbsp;Mahrukh ,&nbsp;Shah Room ,&nbsp;Muhammad Ansar Ahmad ,&nbsp;Muhammad Iftikhar ,&nbsp;Muhammad Farooq Hussain Munis ,&nbsp;Hassan Javed Chaudhary","doi":"10.1016/j.bcab.2024.103388","DOIUrl":"10.1016/j.bcab.2024.103388","url":null,"abstract":"<div><div>Carrier-based biofertilizers, which involve the introduction of plant growth-promoting bacteria into agricultural industry waste materials, are gaining increasing attention due to their profoundly positive impacts on soil health. In current study, various carriers, including dry leaves, sugarcane husk (SCH), rice husk (RH), and a combination/Mixture (MIX) of all three, were employed to support the bacterial strain, <em>Bacillus safensis</em> (SCAL1). This strain was utilized as a bioinoculant in the production of carrier-based biofertilizers. This particular strain was sourced from the Environmental and Microbial Botany Lab at Quaid-i-Azam University in Islamabad. <em>Bacillus safensis</em> strain exhibited notable improvements, evident in a high rate of longevity and larger colony-forming units within the carriers, as confirmed by measurements taken after 21 days of incubation. The drought stress was induced using polyethylene glycol solutions with concentrations of 15% and 25% administrated 6 and 15 days after germination, respectively. Four distinct biofertilizer types were formulated in the study: DL + SCAL1, RH + SCAL1, SCH + SCAL1, and MIX + SCAL1. When applied to <em>Brassica juncea</em> L. plants, all of these biofertilizers demonstrated the ability to mitigate drought stress and enhance the quality of <em>Brassica juncea</em> L. The immobilization onto SCAL1 by using SCH showed the best result compared to the liquid inoculum as carrier materials improve stability and shelf life. Furthermore, our results affirmed that carrier-based biofertilizers improved biochemical and physiological attributes of plants during drought conditions, highlighting their importance as a valuable resource for mitigating drought stress in <em>Brassica juncea</em> L. The findings revealed that carrier-based biofertilizers are an excellent source of diverse phytohormones, which are pivotal in alleviating drought stress.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micropropagation, micromorphological evaluation and genetic homogeneity validation of Cucumis collosus (Rottl.) Cogn.: A wild progenitor of melon","authors":"Sumitra Kumari Choudhary ,&nbsp;Ashok Kumar Patel ,&nbsp;Rekha Chouhan ,&nbsp;Kheta Ram ,&nbsp;Supriya Ambawat ,&nbsp;Smita Shekhawat ,&nbsp;Narpat S. Shekhawat","doi":"10.1016/j.bcab.2024.103378","DOIUrl":"10.1016/j.bcab.2024.103378","url":null,"abstract":"<div><div><em>Cucumis collosus</em> (Rottl.) Cogn., a wild ancestor of melon cultivars, is an underutilized cucurbit of arid horticulture having nutraceutical and therapeutic significance. <em>C. collosus</em> has great potential for enhancing the genetic base of cultivated melon and other cucurbits. This investigation is the first report on a robust and reliable micropropagation system for <em>C. collosus</em> (var. AHK-119) using nodal explants, followed by micromorphological and genetic stability assessment. More than 95% explants showed axillary shoot-bud induction on MS (Murashige and Skoog, 1962) medium containing 0.5 mg L⁻¹ BAP and developed a maximum of 3.2 ± 0.6 shoots measuring 3.2 ± 0.1 cm length. After evaluating the various parameters, the best shoot multiplication (Shoot Number 34.9 ± 1.7, Shoot Length 6.7 ± 0.9 cm) was achieved on MS medium having half-strength of nitrates (825 mg L⁻¹ NH₄NO₃ and 950 mg L⁻¹ KNO₃) + 0.25 mg L⁻¹ 6-benzylaminopurine (BAP) + 0.1 mg L⁻¹ Kinetin (Kin) + 0.1 mg L⁻¹ Indole-3-acetic acid (IAA) + additives (50 mg L⁻¹ ascorbic acid and 25 mg L⁻¹ each of adenine sulfate, L-arginine and citric acid), after 4 weeks. Micro-shoots were rooted using three approaches: <em>In Vitro</em> Continuous Treatment (IVCT), <em>In Vitro</em> Pulse-Treatment (IVPT) and <em>Ex Vitro</em> Pulse Treatment (EVPT). Of these, EVPT approach proved to be most successful in terms of rooting response (80%), root number (6.4 ± 0.9) and root length (5.7 ± 0.7 cm). The foliar micro-morphology analysis of <em>in vitro</em> leaves (IVL), <em>ex vitro</em> leaves (EVL) and mother plant leaves (MPL) demonstrated notable adaptive alterations during the transitional stages and produced structurally stable plants similar to the mother plant. Furthermore, the validation of genetic homogeneity using Start Codon Targeted (SCoT) and Inter Simple Sequence Repeats (ISSR) markers revealed monomorphic banding patterns among the micropropagated and the mother plant. The discussed method can be applied for large-scale commercial production of <em>C. collosus</em> for arid horticulture, urban/vertical farming, breeding purposes and improvement of other cucurbits.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical profiling of Ananas comosus fruit via UPLC-MS and its anti-inflammatory and anti-arthritic activities: In Silico, In Vitro and In Vivo Approach","authors":"Sally E. Khaled ,&nbsp;Faten Mohamed Ibrahim ,&nbsp;Eman Abdelsalam ,&nbsp;Kawkab A. Ahmed ,&nbsp;Asmaa K. Al-Mokaddem ,&nbsp;Wedian El Sayed Ashour ,&nbsp;Amany A. Sleem ,&nbsp;Reda Sayed Mohammed","doi":"10.1016/j.bcab.2024.103393","DOIUrl":"10.1016/j.bcab.2024.103393","url":null,"abstract":"<div><div><em>Ananas comosus</em> [L.] is one of the most appreciated sources of metabolites for nutraceuticals and therapeutics. Traditional use of <em>A. comosus</em> fruit as anti-inflammatory and anti-arthritic agents warrants scientific validation. <em>A. comosus</em> fruit is well-known for its polyphenolic content and antioxidant activity that pose it good candidate in alleviating arthritis. This study aims to investigate the potential of <em>A. comosus</em> fruit as anti-arthritic and anti-inflammatory agent using different approaches in an attempt to reveal the underlying mechanism of action in accordance to its phytoconstituents. Results revealed that total ethanol extract of <em>A. comosus</em> (TEA) has potent <em>in vitro</em> antioxidant, anti-inflammatory and anti-arthritic activities. Also, TEA (500 and 1000 mg/kg) manifested promising <em>in vivo</em> anti-arthritic activity by alleviating paw edema to only 20.2 and 16.4% increase, respectively. High dose of TEA showed significant reduction in inflammatory biomarkers reverting IL- 6 to near normal value (46.93 pg/mL). TEA reversed the induced histopathological damage caused by the adjuvant and inhibit inducible nitric oxide synthase expression. UPLC/QTOF-MS-MS was carried out for metabolite profiling with a total of 53 metabolites identified including hydroxycinnamic acids (HCA), their depsides, glycerides and glycosides derivatives together with hydroxybenzoic acid glycosides, and amino acids. <em>In silico</em> studies displayed inhibitory potential against TNF-α for most of studied HCA derivatives especially dicinnamoyl glycerides and methoxylated HCA. This study is considered the first pharmacological validation of pineapple fruit traditional use. These observed results are attributed to TEA metabolite profile that exhibited favorable pharmacokinetics and drug likeness properties, suggesting their potential as lead drugs.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}