Biocatalysis and agricultural biotechnology最新文献_第3页

Valorisation of biowaste for the production of high-quality irrigation water: A sustainable approach in hydroponic wheat cultivation 生物废弃物的增值生产高质量灌溉用水：水培小麦种植的可持续途径

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2025-07-01 DOI: 10.1016/j.bcab.2025.103675

Younesse El-byari, Mohammed Bendriss Amraoui

{"title":"Valorisation of biowaste for the production of high-quality irrigation water: A sustainable approach in hydroponic wheat cultivation","authors":"Younesse El-byari, Mohammed Bendriss Amraoui","doi":"10.1016/j.bcab.2025.103675","DOIUrl":"10.1016/j.bcab.2025.103675","url":null,"abstract":"<div><div>Faced with the increasing scarcity of water and the challenges of organic waste management, this study proposes an innovative method for valorizing biowaste to produce high-quality irrigation water by combining thermal drying and biofiltration. Three types of biowaste, including potato peels, orange peels, and coffee grounds, were treated using devices with or without biofilters, incorporating ashes derived from <em>Hermetia illucens</em> L. or <em>Cupressus atlantica</em> G. Each thermal cycle operated at 55 °C for 24 h and treated 100 g of fresh biowaste, yielding on average 60 ml of condensed water per cycle. Physicochemical, microbiological, and agronomic analyses revealed that the device without a biofilter produced water rich in biodegradable nutrients (BOD<sub>5</sub>: 1103 mg/l), promoting an average germination rate of 93.22 % and optimal vegetative growth of wheat in hydroponic systems. In contrast, devices equipped with biofilters effectively reduced organic load (COD: 461.66 mg/l for <em>Cupressus atlantica</em> and 70.44 mg/l for <em>Hermetia illucens</em> L.) while eliminating microbial contaminants, with no detectable traces of heavy metals. The <em>Cupressus atlantica G..</em>-based biofilter achieved a high germination rate (94.55 %) due to the controlled release of mineral salts, whereas the <em>Hermetia illucens</em> L<em>.-</em>based biofilter resulted in a germination rate of 85.10 %, highlighting the critical balance between nutrient availability and contaminant reduction. These findings demonstrate the potential of this technology to produce environmentally friendly water suitable for sustainable irrigation and circular resource management. This approach offers a promising solution to address the dual challenges of water scarcity and biowaste valorization.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103675"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144579415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of glutamic acid production capacity of Stenotrophomonas sp. strain CG2 isolated from soil 土壤中窄养单胞菌CG2产谷氨酸能力的研究

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2025-07-01 DOI: 10.1016/j.bcab.2025.103665

Cihat Guner , Ertan Ermis , Kubra Ozkan Guner

{"title":"Investigation of glutamic acid production capacity of Stenotrophomonas sp. strain CG2 isolated from soil","authors":"Cihat Guner , Ertan Ermis , Kubra Ozkan Guner","doi":"10.1016/j.bcab.2025.103665","DOIUrl":"10.1016/j.bcab.2025.103665","url":null,"abstract":"<div><div>Glutamic acid is a widely used amino acid in the food and pharmaceutical industries due to its role as a flavor enhancer and a metabolic precursor. This study aimed to identify glutamic acid-producing bacterial strains from soil samples collected across different regions. Among 262 isolates screened, <em>Stenotrophomonas</em> sp. strain CG2 exhibited the highest production capacity and was identified through 16S rRNA sequencing. Fermentation parameters including pH, temperature, incubation time, and agitation speed were optimized using the Plackett–Burman design, leading to a maximum yield of 3.76 ± 0.65 g/L under optimized conditions (pH 7.0, 30 °C, 200 rpm, 84 h), compared to 2.72 g/L in unoptimized TSB medium. The produced glutamic acid was purified using ion-exchange resin, yielding a recovery efficiency of 53.48 ± 3.28 %, and its identity was confirmed by FT-IR, RAMAN, and LC-MS/MS analysis.</div><div>This study is among the first to systematically explore Stenotrophomonas spp. for glutamic acid biosynthesis under optimized fermentation conditions. The results provide insight into the strain's specific responses to nutrient composition, revealing its potential for future biotechnological applications. By expanding the microbial landscape of amino acid producers, this work offers a foundation for using CG2 in sustainable bioproduction processes, particularly those leveraging food or agro-industrial waste streams.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103665"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Culture optimization for the production and characterisation of exopolysaccharide of Leuconostoc lactis Miki-A3 isolated from traditional fermented rice beverages 传统发酵米酒中乳酸菌Miki-A3胞外多糖的培养优化及特性研究

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2025-07-01 DOI: 10.1016/j.bcab.2025.103689

Riko Kamata, Hikaru Ogura, Ayaka Nakamura, Hajime Takahashi, Tomoyuki Koyama, Takashi Kuda

{"title":"Culture optimization for the production and characterisation of exopolysaccharide of Leuconostoc lactis Miki-A3 isolated from traditional fermented rice beverages","authors":"Riko Kamata, Hikaru Ogura, Ayaka Nakamura, Hajime Takahashi, Tomoyuki Koyama, Takashi Kuda","doi":"10.1016/j.bcab.2025.103689","DOIUrl":"10.1016/j.bcab.2025.103689","url":null,"abstract":"<div><div>Highly viscous exopolysaccharides (EPS) derived from lactic acid bacteria are useful materials for improving the characteristics of food products and are thought to function as soluble dietary fibre. However, their productivity, structure, antioxidant activity, and other functional properties may vary among species and strains. In this study, to clarify the optimal culture conditions for producing highly viscous EPS by <em>Leuconostoc lactis</em> Miki-A3 isolated from rice fermented beverages, the viscosity of the culture medium was measured under various culture conditions. The EPS structures were examined through constituent sugar analysis, attenuated total reflection-Fourier transform infrared spectroscopy, <sup>1</sup>H- and <sup>13</sup>C nuclear magnetic resonance spectroscopy, and gel filtration chromatography. Furthermore, the superoxide anion (O<sub>2</sub><sup>−</sup>) radical scavenging and Fe-reducing capacities of EPS were measured to determine their antioxidant properties. The optimal culture conditions for EPS production in Miki-A3 were 10 % (w/v) sucrose adjusted to an initial pH of 7.5, with incubation at 23 °C, which is lower than the optimal temperature for growth (30–35 °C), for 24 h. Crude EPS showed a high molecular weight (3800 kDa) and consisted exclusively of <em>α</em>(1→6) glucan. The produced EPS was recovered through precipitation in approximately 60 % (v/v) ethanol, which removed small-molecule contamination. High-molecular-weight EPS showed a strong O<sub>2</sub><sup>−</sup> radical scavenging capacity. These results may be useful for the large-scale production of Miki-A3-derived EPS and studies of its potential applications. The expression of temperature-sensitive genes and consideration of fermentation materials require further analysis.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103689"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144571959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of bacterial biomass production using soybean molasses-based medium as a potential component for bean crop inoculant development 以大豆糖蜜为基础的培养基优化细菌生物量生产，作为大豆作物接种剂开发的潜在成分

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2025-07-01 DOI: 10.1016/j.bcab.2025.103668

Ana Cristina Silva da Natividade, Rui dos Santos Ferreira Filho, Janaína Fernandes de Medeiros Burkert, Carlos André Veiga Burkert

{"title":"Optimization of bacterial biomass production using soybean molasses-based medium as a potential component for bean crop inoculant development","authors":"Ana Cristina Silva da Natividade, Rui dos Santos Ferreira Filho, Janaína Fernandes de Medeiros Burkert, Carlos André Veiga Burkert","doi":"10.1016/j.bcab.2025.103668","DOIUrl":"10.1016/j.bcab.2025.103668","url":null,"abstract":"<div><div>This study aimed to optimize bacterial biomass production using a soybean molasses-based medium in shaken flask cultures as a potential component for bean crop inoculant development. <em>Bradyrhizobium yuanmingense</em> SEMIA 6462, <em>Bradyrhizobium pachyrhizi</em> SEMIA 6464, <em>Rhizobium tropici</em> SEMIA 4077, and <em>Rhizobium freirei</em> SEMIA 4080 were evaluated. <em>B. yuanmingense</em> SEMIA 6462 presented the highest biomass production, which was 5.48 g L<sup>−1</sup> after 72 h of cultivation. For this microorganism, a Plackett-Burman (PB) design with ten variables followed by a 2<sup>4</sup> Central Composite Rotatable Design (CCRD) were proposed to optimize biomass production. The variables with the greatest influence were soybean molasses, sodium chloride, calcium chloride dihydrate, and yeast extract concentrations, with the pH adjusted to 7, while dibasic and monobasic potassium phosphates, magnesium sulfate heptahydrate, manganese chloride heptahydrate, and Tween 80 were removed from the medium. The best conditions established by an empirical model and validated by experimental assays were 100 g L<sup>−1</sup> soybean molasses, 0.8 g L<sup>−1</sup> yeast extract, 0.45 g L<sup>−1</sup> sodium chloride, and 0.3 g L<sup>−1</sup> dihydrate calcium chloride. Under these conditions, biomass production reached 38.6 g L<sup>−1</sup>, representing a 604.93 % increase from the initial value (5.48 g L<sup>−1</sup>) while also reducing the medium cost per kilogram of biomass by 70 %. The results demonstrate the efficacy of cultivation conditions in increasing rhizobia biomass production, highlighting the potential of soybean molasses as an alternative and sustainable substrate to produce microbial inoculants.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103668"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144522504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequential optimization for biomass and fucoxanthin enriched carotenoid production of Isochrysis galbana 褐藻等chrysis galbana生物量和富岩藻黄素类胡萝卜素生产的顺序优化

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2025-07-01 DOI: 10.1016/j.bcab.2025.103686

Vijayakumar Maduraimuthu , Shenbhagaraman Ramalingam , Thirunavukkarasu Chinnasamy , Raja Rathinam , Babu Saranam , Jayappriyan Kothilmozhian Ranishree

{"title":"Sequential optimization for biomass and fucoxanthin enriched carotenoid production of Isochrysis galbana","authors":"Vijayakumar Maduraimuthu , Shenbhagaraman Ramalingam , Thirunavukkarasu Chinnasamy , Raja Rathinam , Babu Saranam , Jayappriyan Kothilmozhian Ranishree","doi":"10.1016/j.bcab.2025.103686","DOIUrl":"10.1016/j.bcab.2025.103686","url":null,"abstract":"<div><div>Microalgae are promising sources of high-value bioactive compounds, offering renewable, low-waste, and environmentally friendly alternatives to synthetic chemicals. This study focuses on optimizing the cultivation conditions of <em>Isochrysis galbana</em> to enhance biomass and carotenoid production, with particular emphasis on enhancing fucoxanthin, a potent antioxidant with diverse biological activities. A sequential optimization strategy combining one-factor-at-a-time (OFAT) screening and response surface methodology (RSM) was employed to identify and refine key physicochemical variables. Optimal conditions for biomass production were 2.75 mM nitrate, 0.32 mM phosphate and 23 μE m<sup>−2</sup> s<sup>−1</sup> light intensity, whereas total carotenoids and fucoxanthin yields were maximized at 2.43 mM nitrate, 0.28 mM phosphate, and 28 μE m<sup>−2</sup> s<sup>−1</sup>. Under optimized conditions, biomass reached 1.40 ± 0.05 g L<sup>−1</sup> (volumetric productivity: 0.05 g L<sup>−1</sup> d<sup>−1</sup>), total carotenoids increased to 98.67 ± 1.43 mg g<sup>−1</sup> (4.34 mg L<sup>−1</sup> d<sup>−1</sup>), and fucoxanthin content was enhanced to 31.36 ± 1.55 mg g<sup>−1</sup> (1.38 mg L<sup>−1</sup> d<sup>−1</sup>). These values represent 4.83, 4.47, and 4.24-fold increases, respectively, compared to the unoptimized conditions. The results demonstrate that <em>I. galbana</em> cultivation under optimized conditions is a viable and sustainable approach for the large-scale production of fucoxanthin and other carotenoids. This work supports the potential integration of <em>I. galbana</em>-based systems into biotechnological pipelines targeting functional foods, nutraceuticals, and pharmaceutical applications.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103686"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biogenically synthesized Moringa oleifera leaf extract-derived silver nanoparticles exhibit potent antimicrobial and anticancer effects via oxidative stress and apoptotic pathways in AGS gastric cancer cells 生物合成辣木叶提取物衍生的银纳米颗粒通过氧化应激和凋亡途径在AGS胃癌细胞中表现出强大的抗菌和抗癌作用

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2025-07-01 DOI: 10.1016/j.bcab.2025.103674

Kaavyaunni , S. Antony , Kanagaraj Muthu-Pandian Chanthini

{"title":"Biogenically synthesized Moringa oleifera leaf extract-derived silver nanoparticles exhibit potent antimicrobial and anticancer effects via oxidative stress and apoptotic pathways in AGS gastric cancer cells","authors":"Kaavyaunni , S. Antony , Kanagaraj Muthu-Pandian Chanthini","doi":"10.1016/j.bcab.2025.103674","DOIUrl":"10.1016/j.bcab.2025.103674","url":null,"abstract":"<div><div>Silver nanoparticles (AgNPs) possess anticancer and targeted drug delivery potential. When synthesized biogenically, their biocompatibility and therapeutic efficacy are improved through capping with bioactive compounds. This study explores the anticancer potential of silver nanoparticles (AgNPs) synthesized using bioactive compounds from <em>Moringa oleifera</em> (MOAgNPs) against AGS gastric cancer cells. UV–Vis spectrometry confirmed MOAgNP synthesis with peaks at 255.72 and 301.04 nm, while FT-IR revealed O-H and carbonyl groups crucial for nanoparticle stability; DLS analysis showed a narrow particle size distribution with an average diameter of 30–35 nm, consistent with TEM observations. Microscopy confirmed spherical nanoparticles with a diameter of 30–35 nm, while a zeta potential of −24.9 mV indicated colloidal stability. MOAgNPs demonstrated cytotoxicity with an IC50 of 55.213 μg/mL, inducing ROS generation, apoptosis, and cellular morphology changes. Downregulation of key proteins involved in energy metabolism and apoptosis highlighted MOAgNPs' ability to impair vital cellular functions and enhance the sensitivity of drug-resistant cancer cells, suggesting their potential as effective anticancer agents for gastric cancer treatment.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103674"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144571956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinctive hepatoprotective and anti-inflammation activity of snakefruit vinegar (Salacca zalacca (Gaerth.) Voss) with mixed culture fermentation induced by CCl4 in Wistar rats 蛇果醋独特的保肝抗炎活性用CCl4对Wistar大鼠进行混合培养发酵

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2025-07-01 DOI: 10.1016/j.bcab.2025.103678

Elok Zubaidah , Viera Nu'riza Pratiwi , Tri Dewanti Widyaningsih , Joni Kusnadi

{"title":"Distinctive hepatoprotective and anti-inflammation activity of snakefruit vinegar (Salacca zalacca (Gaerth.) Voss) with mixed culture fermentation induced by CCl4 in Wistar rats","authors":"Elok Zubaidah , Viera Nu'riza Pratiwi , Tri Dewanti Widyaningsih , Joni Kusnadi","doi":"10.1016/j.bcab.2025.103678","DOIUrl":"10.1016/j.bcab.2025.103678","url":null,"abstract":"<div><div>The hepatoprotective efficacy of snakefruit vinegar (Salacca zalacca (Gaerth.) Voss) has not been previously investigated in models of chemically induced liver injury, despite growing interest in the health benefits of fruit vinegars. This study aimed to evaluate the hepatoprotective and anti-inflammatory effects of snakefruit vinegar produced via single- and mixed-culture fermentation in carbon tetrachloride (CCl<sub>4</sub>)-induced hepatotoxicity rats. Twenty male rats were randomly assigned to four groups: healthy control (K−), CCl<sub>4</sub>-induced control (K+), single-culture vinegar treatment (P1), and mixed-culture vinegar treatment (P2). Vinegar was administered orally at 0.75 mL/kg body weight for 28 days, while CCl<sub>4</sub> was injected intraperitoneally during the final 8 days of the intervention. Mixed-culture treatment (P2) significantly reduced serum ALT (43.8 ± 5.94 IU/L), AST (133.6 ± 2.50 IU/L), and MDA (2.18 ± 0.26 mg/L) compared to the CCl<sub>4</sub>-only group (76.4 ± 5.13, 249.4 ± 8.26 IU/L, and 6.49 ± 0.54 mg/L, respectively; p < 0.05). Histopathological analysis revealed improved hepatic architecture in P2, with a damage score of 1, versus a score of 3 in the untreated CCl<sub>4</sub> group. Additionally, treatment with snakefruit vinegar downregulated pro-inflammatory cytokines TNF-α and IL-6 (1.56 ± 0.22 and 1.74 ± 0.15 pg/mL, respectively) and upregulated anti-inflammatory IL-10 (1.22 ± 0.28 pg/mL), in contrast to the K+ group (2.80 ± 0.32, 3.36 ± 0.36, and 0.21 ± 0.22 pg/mL; p < 0.05). These findings confirm that snakefruit vinegar, particularly from mixed-culture fermentation, exerts significant hepatoprotective and anti-inflammatory effects, and may serve as a promising functional food for liver health.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103678"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144571957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green-synthesized nanoparticles: recent trends and innovations towards sustainable agriculture 绿色合成纳米颗粒：可持续农业的最新趋势和创新

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2025-07-01 DOI: 10.1016/j.bcab.2025.103667

Ameni Ben Zineb , Muhammad Fasih Khalid , Mahmoud Gargouri , Juha M. Alatalo , Talaat Ahmed

{"title":"Green-synthesized nanoparticles: recent trends and innovations towards sustainable agriculture","authors":"Ameni Ben Zineb , Muhammad Fasih Khalid , Mahmoud Gargouri , Juha M. Alatalo , Talaat Ahmed","doi":"10.1016/j.bcab.2025.103667","DOIUrl":"10.1016/j.bcab.2025.103667","url":null,"abstract":"<div><div>Environmental challenges posed by conventional chemical methods in agriculture have sparked a revolution in green nanotechnology to provide eco-friendly solutions. In this regard, green-based nanoparticles (GB-NPs) offer significant economic and ecological benefits while reducing the environmental impact. This review dives deep into the synthesis and characterization of GB-NPs, highlighting their key synthesis factors, limitations, and their role in plant stress tolerance. Notably, this review goes beyond previous reports by providing an in-depth analysis of recent research employing GB-NPs for mitigating plant pests and diseases. A Sankey diagram was used to illustrate the studies and quality of evidence on GB-NPs outcomes for the mitigation of phytopathogens during the last ten years, providing valuable insights into their application in mitigating biotic stress. Additionally, to bridge the gap between research and real-world application, a conceptual framework is proposed to facilitate the large-scale adoption of GB-NPs in sustainable agriculture, laying the groundwork for future research and practical implementation.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103667"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144522503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Augmented elimination of cadmium and mercury by Cytobacillus firmus and Paenibacillus massiliensis isolated from heavy metal contaminated soil samples 从重金属污染土壤中分离的坚固胞杆菌和马斯利拟芽孢杆菌增强对镉和汞的去除作用

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2025-07-01 DOI: 10.1016/j.bcab.2025.103669

Ankita Ghosh, Moumita Chakraborty, Diksha Sah, J.P.N. Rai

{"title":"Augmented elimination of cadmium and mercury by Cytobacillus firmus and Paenibacillus massiliensis isolated from heavy metal contaminated soil samples","authors":"Ankita Ghosh, Moumita Chakraborty, Diksha Sah, J.P.N. Rai","doi":"10.1016/j.bcab.2025.103669","DOIUrl":"10.1016/j.bcab.2025.103669","url":null,"abstract":"<div><div>Cadmium and mercury, often classified as heavy metals or toxic elements, are prevalent in the environment due to industrial activities and pollution, posing risks to ecosystems and human health. The current research utilized the metal tolerance abilities of extremely resilient microorganisms, both individually and as part of a consortium, to address the complex issue of heavy metal pollution remediation. These bacterial strains were isolated from contaminated soil and subjected to screening under different treatment conditions. The examination and screening investigations have unveiled an isolated strain as Cd resistant bacteria viz. BS4 (<em>Cytobacillus firmus</em>) which have shown Cd removal up to 82 % and Hg tolerant bacterial isolate namely, BS10 (<em>Paenibacillus massiliensis</em>) with Hg removal efficiency of more than 63 %. Furthermore, the engineered microbial consortium (BS4 + BS10) has proven its capability to enhance the removal of Cd and Hg from soil samples. Extensive validation considered various factors such as pH, temperature, initial concentrations of Cd and Hg, and size of the inoculum, establishing the consortium's biosorption capacity. Structural alterations induced by microbial treatment were evidenced through FESEM and FTIR analysis. The metal-binding proficiency exhibited by this bacterial consortium underscores its potential as an efficient biosorbent for addressing heavy metal contamination.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103669"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of malachite green degradation with response surface methodology in bio-electro-Fenton system 响应面法优化生物电fenton系统对孔雀石绿的降解

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2025-07-01 DOI: 10.1016/j.bcab.2025.103677

Ziyi Yang , Yuying Peng , Kaiwei Mao , Wen Wang

{"title":"Optimization of malachite green degradation with response surface methodology in bio-electro-Fenton system","authors":"Ziyi Yang , Yuying Peng , Kaiwei Mao , Wen Wang","doi":"10.1016/j.bcab.2025.103677","DOIUrl":"10.1016/j.bcab.2025.103677","url":null,"abstract":"<div><div>The bio-electro-Fenton system has been recognized as an energy-efficient and sustainable method for treating malachite green (MG) wastewater. In this study, the operational conditions, including pH, voltage, air flowrate, MG initial concentration and Fe<sup>2+</sup> concentration, were optimized and evaluated by response surface methodology. The model from response surface methodology exhibited a strong correlation between simulated and experimental values, indicating its accuracy and applicability for predicting system performance. Optimal conditions of pH 3, air flowrate 17 mL min<sup>−1</sup>, voltage 0.6 V, MG initial concentration 43 mg L<sup>−1</sup> and Fe<sup>2+</sup> concentration 8 mM were obtained based on the simulated results, along with MG degradation efficiency of 98.9 %. The formation of mature biofilm on bio-anode in bio-electro-Fenton system provided evidence of the stability for long-term operation. Through analysis of intermediates, the degradation pathways of MG were presumed. MG was degraded by two pathways, (i) ∙OH demethylation, the replacement of methyl with H; (ii) ∙OH oxidation, benzene ring was removed and C-C bonds were broken.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103677"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144571958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0