Biocatalysis and agricultural biotechnology最新文献

{"title":"Bioactive compounds, antimicrobial properties of Açaí (Euterpe oleracea mart.) and its processing residues – Insights for smart packaging and green chemistry","authors":"Lucas Gomes Carvalho ,&nbsp;Anna Paula Azevedo de Carvalho ,&nbsp;Carlos Adam Conte-Junior","doi":"10.1016/j.bcab.2025.103540","DOIUrl":"10.1016/j.bcab.2025.103540","url":null,"abstract":"<div><div><em>Euterpe oleracea</em> Mart. is a palm tree native to the Brazilian Amazon, an important food source producing açaí fruit, rich in flavonoids of antioxidant and antimicrobial activities. Açaí oil pulp is a rich source of lipophilic antioxidants, rich in mono- and polyunsaturated fatty acids (60% oleic acid and 12% linoleic acid), vitamins A, E, D, and phytosterols. Açaí fruit comprises 10% of pulp and 90% of residues. Its processing also generates the seed as a by-product, and recent studies showed açaí seed oil (∼6% yields) rich in flavonoids, anthocyanins, oleic, linoleic, myristic, and palmitic acid. Herein, we provided a comprehensive overview of antimicrobial activity and bioactive compounds in açaí. We identified technical approaches to improve antimicrobial activity as the effect of solvent extract; combining bioactive compounds; encapsulation of açaí oil in inclusion complexes with potential as excipients and carriers; açaí oil as oily portion of nanoemulsions; incorporation in edible films and coatings to biodegradable packaging materials; açaí extract as natural pH indicator for colorimetric sensing in smart packaging; and synergism with non-thermal food processing methods. Phenolic acids and cyanidins were mainly found as bioactive compounds. Açaí showed antibacterial, antifungal, antiviral, and antiplasmodial activities and synergism with other antimicrobial drugs. Polyphenols of açaí can survive <em>in vitro</em> intestinal digestion and exert anti-genotoxic effects on gut health. We also identified açaí seed waste extracts in new insights for green synthesis in producing novel nanomaterials and intelligent packaging of antioxidant and antimicrobial potential.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103540"},"PeriodicalIF":3.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological response of tomato plants grown in saline and non-saline soils to foliar application of SiO2/TiO2 nanocomposites","authors":"Gisela Adelina Rolón-Cárdenas,&nbsp;Vicente Rodríguez-González","doi":"10.1016/j.bcab.2025.103536","DOIUrl":"10.1016/j.bcab.2025.103536","url":null,"abstract":"<div><div>Silicon dioxide (SiO₂) and titanium dioxide (TiO₂) nanoparticles have been shown to enhance growth and mitigate the adverse effects of salinity stress in various crops. This study investigated the effects of SiO₂/TiO₂ nanocomposites (NCs) on tomato plants (<em>Solanum lycopersicum</em>) under both normal and salt-stress conditions. For this purpose, tomato plants were irrigated with 0, 60, or 120 mM NaCl solutions for 30 days. Fifteen days after initiating the salt treatment, SiO₂/TiO₂ NC suspensions at concentrations of 0, 50, 100, and 500 mg/L were applied via foliar spray. The results showed that, under non-saline conditions, foliar application of SiO₂/TiO₂ NCs significantly increased the number of leaves, shoot dry weight, total chlorophyll, carotenoid content, soluble sugars, anthocyanins, and total phenolic content in tomato plants. Under saline conditions, the positive effects of SiO₂/TiO₂ NCs varied depending on the NaCl concentration in the soil. In plants exposed to 60 mM NaCl, SiO₂/TiO₂ NCs improved shoot fresh weight, total chlorophyll content, proline and soluble sugar levels, catalase activity and reduced H₂O₂ content, as well as increased total phenolic and anthocyanin content. These findings suggest that the SiO₂/TiO₂ NC application increases biomass production and improves the salt tolerance of tomato plants. Therefore, these nanomaterials could be a promising option for promoting tomato growth in slightly and moderately saline soils.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103536"},"PeriodicalIF":3.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143464711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical composition, bioactive compounds, and antioxidant activities of different parts of Gomphostemma ovatum","authors":"Gouri Rani Baglary,&nbsp;Rangina Brahma,&nbsp;Anuck Islary,&nbsp;Santosh Kumar","doi":"10.1016/j.bcab.2025.103538","DOIUrl":"10.1016/j.bcab.2025.103538","url":null,"abstract":"<div><div>This study presents a comprehensive investigation of phytochemical composition, bioactive and nutritional compounds, as well as antioxidant activities of different parts of <em>Gomphostemma ovatum</em> plants. The leaf, stem, and roots of <em>Gomphostemma ovatum</em> were analysed and results showed that the root of the plant has higher carbohydrate (56%), protein (10.62%), and fibre (16.13%) contents. Mineral analysis displayed leaf had higher aluminum (3376 μg/kg), potassium (839 μg/kg), magnesium (3731 μg/kg), and manganese (4112 μg/kg), whereas stem had the highest calcium (2572 μg/kg), chromium (23 μg/kg), copper (23 μg/kg), iron (443 μg/kg), and zinc content (144 μg/kg), while root had the highest sodium (1151 μg/kg) contents. Phytochemical screenings showed the presence of flavonoids, phenols, alkaloids, terpenoids, glycosides, and anthraquinones. GC-MS and LC-MS/MS profiling revealed that there is a presence of several bioactive compounds exhibiting antimicrobial, antioxidant and anti-inflammatory properties few of which are 1-methylene-2b-hydroxymethyl-3,3-dimethyl-4b-(3-methylbut-2-enyl)-cyclohexane, tricosanal, chrysoeriol, catechin, peptide etc. The leaf sample had higher total flavonoid content (TFC), total phenolic content (TPC), and antioxidant activities compared to stem, and root of the plant. This comprehensive study not only corroborates the nutritional significance of <em>Gomphostemma ovatum</em> but also highlights the potential for future drug discovery and development, emphasizing the importance of integrating traditional knowledge with modern scientific research in the pursuit of effective healthcare solutions.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103538"},"PeriodicalIF":3.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced upcycling of spent hen lipids using engineered-enzymatic cascade","authors":"Elias Rietzschel,&nbsp;Kethmi Jayawardhane,&nbsp;Muhammad Zubair,&nbsp;Guanqun Chen,&nbsp;Aman Ullah","doi":"10.1016/j.bcab.2025.103534","DOIUrl":"10.1016/j.bcab.2025.103534","url":null,"abstract":"<div><div>A novel enzymatic epoxidation process has been carried out for the first time, utilizing lipid and fatty acid methyl esters (FAMEs) obtained from spent hens and olives. In this process, an engineered enzymatic cascade was designed to facilitate epoxidation by leveraging the catalytic activity of galactose oxidase and lipase B. The primary objective of the study was to develop a reusable enzyme cascade for the epoxidation of lipids extracted from spent hens employing microwave technology. First of all, the Strep-tag II-galactose oxidase-macaque histatin (StrepII-GalOx-MH) complex was successfully bound to commercial Immbodead 150 particles with pre-immobilized lipase B. Furthermore, our study demonstrated that the StrepII-GalOx-MH was stable in a two-phase system consisting of sodium-phosphate buffer (100 mM, pH 7) and toluene in a 1:2 mixture. Thus, this complex showed 10 % less activity after 72 h of incubation compared to the control incubated in sodium phosphate buffer. The structural analysis of the extracted lipids and FAMEs obtained from spent hens and olives after incubation was performed using ATR-FTIR and GC-MS. The results revealed a successful epoxidation evident from the decreased wavenumbers at ∼3010 cm⁻¹(double bond CH stretch) and ∼1654 cm⁻¹ (double bond C-C stretch), while an increase in the two oxirane regions at ∼1497-1432 cm⁻¹ (oxirane C-C stretching), and 862-762 cm⁻¹ (oxirane C-O-C stretching). After 48 h of incubation, the average conversion of all spent hen FAMEs in the Erlenmeyer flask was 77.0 ± 3.5 % and 59.1 ± 2.2 % when the glass vessel was used. Finally, we demonstrated the reusability and functionality of the enzyme cascade for another 24 h. The successful development of an alternative, biobased approach to produce epoxides from underutilized waste highlights the promising prospect of biotechnology to achieve a circular economy.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103534"},"PeriodicalIF":3.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143438216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of genetic diversity using morphometric, molecular and digital imaging parameters in castor (Ricinus communis L.)","authors":"C. Deepika ,&nbsp;S.R. Venkatachalam ,&nbsp;A. Yuvaraja ,&nbsp;P. Arutchenthil ,&nbsp;N. Indra ,&nbsp;V. Ravichandran ,&nbsp;P. Veeramani ,&nbsp;P. Kathirvelan","doi":"10.1016/j.bcab.2025.103525","DOIUrl":"10.1016/j.bcab.2025.103525","url":null,"abstract":"<div><div>Castor (<em>Ricinus communis</em> L.) is highly remunerative industrially important non-edible oilseed crop with immense and diversified usage. A study was conducted with 26 diverse castor genotypes (20 pistillate and six monoecious) to evaluate diversity using 17 morphometric characters and 22 SSR (simple sequence repeat) markers. Cluster analysis using both hierarchical (Euclidean distance matrix using the Ward method) and non-hierarchical (K means) method grouped the castor lines into five clusters. The genotypes YTP 1, JP 65, DPC 19 and TMV 5 were genetically wide and could be explored further during cross breeding to generate superior recombinants. Principal component analysis based on eigen vectors revealed that the first six components cumulatively explained 86.17% of the total variation. Association analysis exhibited positive relation among seed yield attributing traits. Using 22 microsatellite markers 16 (72.72 %) were polymorphic. The molecular weight (bp) of the amplified products differed from 120 (RCM 10) to 300 bp (RCM 7). A total number of alleles/loci were 63 alleles with the average of 3.94. With a mean value of 0.57, the highest gene diversity (0.75) was for RCM 12 while it was lowest (0.28) for RCM 43. Based on PIC (polymorphic information content) values, 0.26 (RCM 43) to 0.71 (RCM 12), 10 markers were highly informative. The pistillate lines Geeta, M 574 and DPC 24 were diverse and placed in distinct clusters. The findings revealed that both phenotypic and molecular markers should be deployed to capture the actual genetic diversity and to identify promising genotypes to reap maximum heterosis through hybridization.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103525"},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological application of African mustard (Brassica tournefortii): LC-ESI-MS/MS in synergism with featured-based molecular networking, antioxidant and anti-inflammatory propensities","authors":"Nermin A. Ragab ,&nbsp;Faten M. Ibrahim ,&nbsp;Mona O. El Shabrawy ,&nbsp;Mohamad Fawzi Mahomoodally ,&nbsp;Mona M. Marzouk","doi":"10.1016/j.bcab.2025.103533","DOIUrl":"10.1016/j.bcab.2025.103533","url":null,"abstract":"<div><div>The African mustard, <em>Brassica tournefortii,</em> is a traditional medicinal food plant growing broadly on the Mediterranean coastline from El-Saloum to the Sinai Peninsula in the Egyptian Desert. In addition to its culinary uses, it is regarded as a very effective therapeutic herb due to its antioxidant propensities based on phenolic constituents, glucosinolates, and carotenoids. This study endeavored to spotlight the biotechnological application of <em>B. tournefortii via</em> LC-ESI-MS/MS in synergism with molecular networking (-MN). Aqueous ethanol extract of <em>B. tournefortii</em> (BAEE) was subjected to <em>in vitro</em> biological evaluations (antioxidant and anti-inflammation evaluations). Eighty compounds were annotated comprising 74 species-first detections, with the prevalence of flavonol-<em>O</em>-glycosides, followed by hydroxycinnamic and benzoic acids then phospholipids. Additionally, the BAEE showed DPPH and NO radical scavenging inhibition with an IC₅₀ (74 ± 1.01 and 99.87 ± 0.68 μg/ml), compared to ascorbic acid (201 ± 0.98 and 75.25 ± 0.87 μg/ml) and BHT (83 ± 1.84 and 63.35 ± 0.91 μg/ml), respectively. Moreover, <em>in vitro</em> anti-inflammatory evaluation showed that BAEE gave potent inhibition of COX-1 and COX-2 with IC₅₀ (2.9 ± 0.06 and 0.93 ± 0.058 μg/ml) compared with Celecoxib (48 ± 0.52 and 1.09 ± 0.01 μg/ml), Indomethacin (27 ± 0.1 and 0.762 ± 0.003 μg/ml), and Ibuprofen (0.97 ± 0.052 and 15 ± 0.612 μg/ml), respectively. The identified metabolites could be responsible for the observed antioxidant and anti-inflammatory potentials that tend to advocate for a dire need for further <em>in vivo</em> studies.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103533"},"PeriodicalIF":3.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143520589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transforming leather waste into NPK fertilizers: A sustainable approach to boosting malabar spinach (Basella alba) growth","authors":"Farhana Sharmin ,&nbsp;Md Abu Sayid Mia ,&nbsp;Sumaiya Mim ,&nbsp;Mustafizur Rahman Naim ,&nbsp;Umme Habiba Bodrun Naher","doi":"10.1016/j.bcab.2025.103529","DOIUrl":"10.1016/j.bcab.2025.103529","url":null,"abstract":"<div><div>The leather industry generates significant solid waste, posing serious environmental challenges. This study presents a sustainable approach to mitigating this issue by developing NPK-enriched organic fertilizers from chrome-tanned leather shaving wastes (CTLSWs), chicken and fish bone meal, and biochar derived from banana and orange peels. CTLSWs were treated with 10% NaOH for 24 h, successfully removing 99.98% of chromium. Two fertilizer formulations were developed: <strong>F-1</strong>, composed of dechromed collagen, chicken bone meal, and banana peel biochar in equal proportions, and <strong>F-2</strong>, containing dechromed collagen, fish bone meal, and orange peel biochar in a different ratio. Both formulations were characterized using SEM-EDS and FTIR analyses and evaluated for their impact on Malabar spinach (Basella alba) growth. Among the formulations, <strong>F-1 exhibited superior performance</strong>, increasing plant height by 23.43%, the number of leaves by 73.33%, leaf area by 67.54%, and branch numbers by 96.7% compared to the control. It also resulted in the highest dry matter accumulation in shoots (75.56%) and roots (9.82%), along with the best vigor index and germination rate. Although <strong>F-2 also enhanced plant growth</strong>, it was less effective than F-1. Both formulations improved nutrient uptake while keeping chromium levels in leaves within WHO safety limits. This study demonstrates an innovative and eco-friendly method for transforming hazardous leather waste into highly effective organic fertilizers. <strong>F-1 emerged as a promising alternative to chemical fertilizers</strong>, offering a sustainable solution for enhancing agricultural productivity while addressing environmental concerns.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103529"},"PeriodicalIF":3.4,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143579353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the accumulation of poly-β-hydroxybutyrate in the marine cyanobacterium Nostoc sp. BDU 00591: A comprehensive study on extraction, stimulation and characterisation","authors":"Anjali P S,&nbsp;Sudatta Maity,&nbsp;Nirupama Mallick","doi":"10.1016/j.bcab.2025.103488","DOIUrl":"10.1016/j.bcab.2025.103488","url":null,"abstract":"<div><div>Poly-<em>β</em>-hydroxybutyrate (PHB) is a biopolymer with significant potential in biotechnology and biomedicine due to its biodegradability and biocompatibility nature. Although marine cyanobacteria outperform other resources in terms of water footprint, there are few reports on PHB accumulation in marine cyanobacteria. Thus, the present study aimed to evaluate the PHB production in a marine cyanobacterium, <em>Nostoc</em> sp. BDU 00591, from which 54.8 mg L^-1 PHB yield with a content of 6.8% of dry cell weight (dcw), was obtained on the 21st day of incubation under control laboratory conditions. Subsequently, the impact of varying initial culture density, phosphate (P) starvation, and supplementation with organic carbon sources like citrate and acetate at varied concentrations (0.2–0.6% w/v) were evaluated for PHB accumulation, where 4.3-fold higher content under P-starved conditions and ∼2-fold rise in yield under 0.4% acetate and 0.6% acetate supplementations than the control condition was recorded. Therefore, to simultaneously augment PHB yield and content in <em>Nostoc</em> sp., combined studies were performed incorporating P-starved conditions along with 0.4% acetate and 0.6% citrate separately, where the combination of 0.4% acetate supplementation + P-starved conditions increased PHB yield by 5.6-fold and content by 5.9-fold compared to the control. In addition, the incubation period was reduced to 14 days, resulting in an 8.5-fold higher PHB productivity in the test cyanobacterium. Furthermore, characterisation of the obtained PHB by gas chromatography confirmed the presence of homopolymer <em>β</em>-hydroxybutyrate, while thermal analysis through differential scanning calorimetry revealed the melting and crystallisation temperatures as 173.2 °C and 85.8 °C, respectively, thus exhibiting its flexibility and rigidity in different applications.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103488"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-driven synthesis of silver nanoparticles using Erythroxylum coca extract for catalytic reduction of 4-nitrophenol","authors":"Guadalupe García Romero ,&nbsp;Víctor Fabian Ruíz Ruíz ,&nbsp;Alien Blanco Flores ,&nbsp;Helen Paola Toledo Jaldin ,&nbsp;Alfredo Rafael Vilchis-Nestor ,&nbsp;Delia M. Ávila-Márquez ,&nbsp;Delfino Reyes Contreras","doi":"10.1016/j.bcab.2025.103492","DOIUrl":"10.1016/j.bcab.2025.103492","url":null,"abstract":"<div><div>The light-driven synthesis of silver nanoparticles (AgNPs) and their effect on the catalytic reduction of 4-nitrophenol were successfully investigated in this study. AgNPs were synthesized using a biosynthetic approach with an extract prepared from the <em>Erythroxyl</em><em>um</em> <em>coca</em> plant. The resulting nanoparticles exhibited polydispersity in size distribution, with predominantly quasispherical morphologies. Notably, the AgNPs synthesized with exposure to light using <em>Erythroxylum coca</em> extract demonstrated the most efficient catalytic reduction of 4-nitrophenol, achieving a concentration decrease of up to 60%, indicating a higher rate constant than the non-exposed group. This enhanced performance is attributed to the smaller size of the nanoparticles. Additionally, the study revealed that the synthesis process with <em>Erythroxylum coca</em> extract varied under different light conditions, suggesting that the wavelength of electromagnetic radiation is a crucial variable for future research. These findings underscore the potential of light-driven biosynthesis using plant extracts in developing efficient catalytic materials for green chemistry applications.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103492"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143147905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Smilax fluminensis extract and fractions on seed germination and seedling development of mono and eudicotyledonous species","authors":"Lucas Santos Azevedo ,&nbsp;Ana Hortência Fonsêca Castro ,&nbsp;Katyuce de Souza Farias ,&nbsp;Vanessa Samúdio Santos Zanuncio ,&nbsp;Denise Brentan Silva ,&nbsp;Luciana Alves Rodrigues dos Santos Lima","doi":"10.1016/j.bcab.2025.103523","DOIUrl":"10.1016/j.bcab.2025.103523","url":null,"abstract":"<div><div>The species <em>Smilax fluminensis</em> Steud. (Smilacaceae), popularly known as “cipó-quina” and “ijuapeca guasu”, originates from Brazil and has several uses in folk medicine and indigenous peoples for the treatment of persistent wounds, arthritis, bone pain, urinary problems, and rheumatism, as well as being used as a blood purifier, tonic, antipyretic and antisyphilitic. The aim of this study was to assess the influence of <em>Smilax fluminensis</em> extract and fractions on seed germination and seedling development of mono and eudicotyledonous species. Ethyl esters and phytol were characterized as the major compounds found in hexane fraction. In the polar samples, the main compounds annotated were kaempferol, luteolin and quercetin derivatives, and saponins. The ethanol extract (EE) and hexane (HEXF), dichloromethane (DCMF) and hydroethanol (HIDF) fractions increased the <em>in vitro</em> germination of <em>Allium cepa</em> seeds compared to controls, mainly at 125 μg/mL and 1000 μg/mL (100% germination) for EE and at 1000 μg/mL for HIDF. In addition, in the presence of EE at 250 μg/mL seedlings presented a greater number of roots, length and dry mass compared to 2,4-D and BAP. Ethyl acetate fraction (EACF) inhibited seed germination and seedling development. In <em>Nicotiana tabacum</em>, 100% germination was observed in the presence of EE at 125 μg/mL, 250 μg/mL and 500 μg/mL, when compared to the controls. However, the seedlings reduced the number of nodes, shoots and leaves, the length and dry mass of the stem and roots. In general, the fractions inhibited the germination of <em>N. tabacum</em> seeds, except 250 μg/mL of HEXF and 750 μg/mL of HIDF, where 100% germination was observed. The fractions also reduced the seedlings development, mainly at 750 μg/mL and 1000 μg/mL, compared to the controls. Thus, this study suggests that the extract and fractions of <em>S. fluminensis</em> exhibited an effect more similar to plant hormones on <em>A. cepa</em> (monocotyledonous) and bioherbicide on <em>N. tabacum</em> (eudicotyledonous), and may therefore play an have an important role in sustainable agriculture.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"64 ","pages":"Article 103523"},"PeriodicalIF":3.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143394709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}