Biocatalysis and agricultural biotechnology最新文献_第3页

Generation of a baby clam (Corbiculidae sp.) meat protein hydrolysate with calcium-binding activity, evaluation of its characteristics, and identification of a novel calcium-binding hexapeptide (LPLTII) applicable as a calcium chelator 具有钙结合活性的蛤仔肉蛋白水解产物的生成、特性评价及一种可作为钙螯合剂的新型钙结合六肽（LPLTII）的鉴定

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2025-09-06 DOI: 10.1016/j.bcab.2025.103770

Tam Dinh Le Vo , Hung Hoa Lam , Hanh Thi Hong Tran , Du Huy Nguyen , Bao Chi Vo

{"title":"Generation of a baby clam (Corbiculidae sp.) meat protein hydrolysate with calcium-binding activity, evaluation of its characteristics, and identification of a novel calcium-binding hexapeptide (LPLTII) applicable as a calcium chelator","authors":"Tam Dinh Le Vo , Hung Hoa Lam , Hanh Thi Hong Tran , Du Huy Nguyen , Bao Chi Vo","doi":"10.1016/j.bcab.2025.103770","DOIUrl":"10.1016/j.bcab.2025.103770","url":null,"abstract":"<div><div>Our research focused on generating a protein hydrolysate from baby clam (<em>Corbiculidae</em> sp.) meat. The resulting product had a hydrolysis degree (DH) of 85.54 ± 2.25 % and demonstrated a calcium-binding capacity (CaBC) of 1085.82 ± 10.41 μg Ca<sup>2+</sup>/g protein. A comprehensive analysis of the hydrolysate was conducted, examining its amino acid profile, functional properties (including solubility, heat resistance, foaming and emulsifying capabilities, and liquid retention characteristics), and bioactivity stability across various pH levels and temperatures. The hydrolysate was then fractionated using centrifugal devices with membranes of different molecular weight cut-offs (30, 10, 3, and 1 kDa). Among the five resulting peptide fractions, the <1 kDa fraction showed the highest CaBC at 4594.87 ± 61.31 μg Ca<sup>2+</sup>/g protein. This fraction underwent tandem mass spectrometry for peptide sequencing. The analysis identified a calcium-binding peptide (668.45 Da) and its amino acid sequence of Leucine-Proline-Leucine-Threonine-Isoleucine-Isoleucine (LPLTII). <em>In silico</em> methods suggested this peptide is neither toxic nor allergenic. Molecular docking simulations indicated that Thr<sub>4</sub> and Leu<sub>3</sub> are the primary calcium-binding sites on the peptide. The calcium binding hydrolysate and its fractions could be used as calcium binders, added in functional food or nutraceutical. Besides, they could be fortified into food products to enrich required amino acids. They could be used for food quality enhancement as well.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103770"},"PeriodicalIF":3.8,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145020384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endophytic Aspergillus sp. from Phragmites australis: A source of DNA gyrase inhibitors with antibiofilm, antioxidant, and cytotoxic potentials: in vitro supported by in silico 芦苇内生曲霉：具有抗生物膜、抗氧化和细胞毒性的DNA旋切酶抑制剂的来源：在体外由硅支持

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2025-09-06 DOI: 10.1016/j.bcab.2025.103745

Sarah I. Bukhari , Mosad A. Ghareeb , Maha Eid Omran , Omnia Karem M. Riad , Heba Mohammed Refat M. Selim , Ghadeer Bukhari , Nervana S. Diab , Maged M. Mahmoud , Nariman R. Soliman , Asmaa Saleh , Ahmed A. Hamed

{"title":"Endophytic Aspergillus sp. from Phragmites australis: A source of DNA gyrase inhibitors with antibiofilm, antioxidant, and cytotoxic potentials: in vitro supported by in silico","authors":"Sarah I. Bukhari , Mosad A. Ghareeb , Maha Eid Omran , Omnia Karem M. Riad , Heba Mohammed Refat M. Selim , Ghadeer Bukhari , Nervana S. Diab , Maged M. Mahmoud , Nariman R. Soliman , Asmaa Saleh , Ahmed A. Hamed","doi":"10.1016/j.bcab.2025.103745","DOIUrl":"10.1016/j.bcab.2025.103745","url":null,"abstract":"<div><div>A fungal isolate, AHMSH2, was obtained from <em>Phragmites australis</em> collected in El-Beheira, Egypt, and identified as <em>Aspergillus</em> sp. via 18S rRNA gene sequencing (GenBank: PQ432869.1). Large-scale fermentation on potato dextrose broth followed by GC-MS profiling revealed 49 metabolites, with canthaxanthin as a dominant compound. Crude extracts exhibited selective antimicrobial activity, with moderate inhibition against <em>Escherichia coli</em> and limited effects on other pathogens. Notably, antibiofilm assays exhibited a marked suppressive effect on <em>Escherichia coli, Staphylococcus aureus</em>, and <em>Bacillus</em> subtilis. Antioxidant screening via DPPH radical scavenging yielded a maximum inhibition of 43.01 %. DNA Gyrase-B inhibition assays confirmed a potent IC<sub>50</sub> of 5.23 μg/mL, while molecular docking of canthaxanthin indicated a stable binding affinity of −8.1 kcal/mol with essential interactions at the ATP-binding pocket. Additional docking against OMPX (1QJ8) highlighted hydrophobic and hydrogen bond contributions. In vitro, cytotoxicity showed selective effects on UO-31 renal cancer cells (IC<sub>50</sub> = 26.58 μg/mL). ADME and toxicity profiling revealed favorable intestinal absorption and metabolic stability, though lipophilicity and CYP3A4 interaction warrant formulation adjustment. The extract's multi-target potential highlights <em>Aspergillus</em> sp. AHMSH2 is a promising source of pharmacologically active metabolites.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103745"},"PeriodicalIF":3.8,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of geometry parameters of packed-bed reactor on continuous orange juice clarification using pectinase immobilized on genipin-activated chitosan beads 填充床反应器几何参数对用皂素活化壳聚糖球固定化果胶酶连续澄清橙汁的影响

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2025-09-04 DOI: 10.1016/j.bcab.2025.103761

Vinícius Zimmermann , Eli Emanuel Esparza-Flores , Carolina Pedroso Partichelli , Vitor Manfroi , Rafael C. Rodrigues

{"title":"Impact of geometry parameters of packed-bed reactor on continuous orange juice clarification using pectinase immobilized on genipin-activated chitosan beads","authors":"Vinícius Zimmermann , Eli Emanuel Esparza-Flores , Carolina Pedroso Partichelli , Vitor Manfroi , Rafael C. Rodrigues","doi":"10.1016/j.bcab.2025.103761","DOIUrl":"10.1016/j.bcab.2025.103761","url":null,"abstract":"<div><div>This study evaluated five distinct packed-bed reactors for orange juice clarification using pectinase immobilized in genipin-activated chitosan beads. The tested reactors were divided into two groups for observation: those with the same volume (40 mL – varying radius and height) and those with the same radius (0.80 cm – varying height and consequently the volume). Flow tests revealed performance disparities in equal-volume reactors, attributed to uneven substrate distribution due to geometric parameters, highlighting that substrate accumulation occurs unevenly in reactors with limited surface area per volume unit. Reactors with smaller radii exhibited 45 % lower clarification efficiency, suggesting that reduced surface area and flow path limitations create zones of higher substrate concentration. No differences were observed in reactors with the same radius. Notably, stability trials demonstrated sustained activity, maintaining over 50 % of the initial levels even after 100 h. However, fruit juice clarification stability displayed particle accumulation, formation of dead zones within the reactor, and clogging, hindering juice flow completely. A promising solution was altering pump orientation, redirecting juice flow (“up-down”), reducing deposition, and extending testing to 80 h until clarification ceased. Reactor design emerged as pivotal in continuous juice clarification within packed-bed systems. Particle accumulation within the reactor substantially hampered performance.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103761"},"PeriodicalIF":3.8,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergism and organogenesis using cytokinins, auxins and LEDs on apical meristems of Ficus carica L. cv. Red Libyan 细胞分裂素、生长素和发光素对无花果根尖分生组织的协同作用及器官发生。红色的利比亚

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2025-09-04 DOI: 10.1016/j.bcab.2025.103756

Y.H. Kho , W.Q. Pang , C.S. Lai , A.R. Parab , B.L. Chew , B.R. Pandian , S. Subramaniam

{"title":"Synergism and organogenesis using cytokinins, auxins and LEDs on apical meristems of Ficus carica L. cv. Red Libyan","authors":"Y.H. Kho , W.Q. Pang , C.S. Lai , A.R. Parab , B.L. Chew , B.R. Pandian , S. Subramaniam","doi":"10.1016/j.bcab.2025.103756","DOIUrl":"10.1016/j.bcab.2025.103756","url":null,"abstract":"<div><div>Owing to their rich nutrient reserves, figs and their value-added products are commercially significant, with the Red Libyan cultivar of common fig (<em>Ficus carica</em> L.) distinguished by its bright red fruit. In micropropagation studies, organogenesis occurs because of the synergistic interaction of abiotic factors with both exogenous and endogenous plant hormones. Therefore, in the present study, we aim to investigate the synergistic interactions among three abiotic probes for growth and development namely, cytokinins, auxins and light emitting diodes (LEDs). Results showed that 20 μM TDZ was the most effective treatment for increasing shoot height. Root induction experiments revealed that media containing 5 μM IAA along with endogenous hormones synergistically produced optimal roots with a 62 % success rate. Synergistic effects of cytokinin (20 μM TDZ), auxin (1 μM NAA) and red LED produced healthier plantlets. Combination of Red + blue LED was optimal to produce plants with higher chlorophyll and carotenoid pigment concentration. This clearly suggests positive synergism due to the addition of a single abiotic probe in the combination treatment of hormones and light. Histological analysis revealed the nature and characteristics of plantlets growing under the synergistic effects of cytokinin, auxin and LEDs. This research contributes to the understanding of plant interactions with abiotic probes such as hormones and impact of LED for organogenesis in micropropagation experiments.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103756"},"PeriodicalIF":3.8,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145020481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the regulatory role of seasonal changes in soil and microbial communities on Codonopsis pilosula physiology 探讨土壤和微生物群落的季节变化对党参生理的调节作用

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2025-09-04 DOI: 10.1016/j.bcab.2025.103765

Ning Zhu , Yumeng Zhou , Mingjun Yang , Feifan Leng , Xiaoxu Lv , Jixiang Chen , Wen Luo , Yonggang Wang

{"title":"Investigating the regulatory role of seasonal changes in soil and microbial communities on Codonopsis pilosula physiology","authors":"Ning Zhu , Yumeng Zhou , Mingjun Yang , Feifan Leng , Xiaoxu Lv , Jixiang Chen , Wen Luo , Yonggang Wang","doi":"10.1016/j.bcab.2025.103765","DOIUrl":"10.1016/j.bcab.2025.103765","url":null,"abstract":"<div><div>Seasonal variations significantly shape soil-microbe-plant interactions, thereby influencing plant growth and metabolic adaptations. Understanding these dynamics is crucial, particularly for medicinal plants like <em>Codonopsis pilosula</em>. This study explored the seasonal links between soil physicochemical properties, <em>C. pilosula</em> physiological traits, and its root-associated microbiome (bacteria via 16S rRNA, fungi via ITS) across four seasons. Distinct seasonal patterns emerged: the growth rate of roots (length and diameter) was significantly higher in autumn and winter, whereas sugar content was highest in spring and summer. Microbial diversity also shifted seasonally; endophytic bacterial diversity was greater in autumn/winter, while rhizosphere fungal diversity peaked in spring/summer. Correlation analyses revealed that key soil properties (e.g., pH, EC, OM), likely driven by seasonal climate shifts, significantly structured the root microbiome and influenced plant physiology. Specifically, certain endophytic bacteria (<em>Exiguobacterium</em>, <em>Bacillus</em>, <em>Acinetobacter</em>) positively correlated with root growth, while the rhizosphere fungus <em>Mortierella</em> correlated positively with sugar content. These findings suggest a seasonal resource allocation strategy in <em>C. pilosula</em>: spring/summer conditions favor sugar accumulation, potentially linked to <em>Mortierella</em> activity, whereas autumn/winter conditions promote accelerated root development, possibly mediated by beneficial endophytic bacteria. Overall, this research elucidates the adaptive physiological strategies of <em>C. pilosula</em> driven by seasonal variations in the soil-microbiome environment.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103765"},"PeriodicalIF":3.8,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of biogenic zinc oxide nanoparticles from Sonneratia ovata leaf extract for multivariate factorial photocatalytic and biological activities 海桑叶提取物生物氧化锌纳米颗粒光催化活性及生物活性的多因子评价

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2025-09-04 DOI: 10.1016/j.bcab.2025.103759

Kalidasan K , Chavanich S , Jagan K.S.G. , Surendhiran S , Naren Vidaarth T.M. , Karthik S , Kathiresan K , Jean Wan Hong Yong , Viyakarn V

{"title":"Evaluation of biogenic zinc oxide nanoparticles from Sonneratia ovata leaf extract for multivariate factorial photocatalytic and biological activities","authors":"Kalidasan K , Chavanich S , Jagan K.S.G. , Surendhiran S , Naren Vidaarth T.M. , Karthik S , Kathiresan K , Jean Wan Hong Yong , Viyakarn V","doi":"10.1016/j.bcab.2025.103759","DOIUrl":"10.1016/j.bcab.2025.103759","url":null,"abstract":"<div><div>The presence of dyes from industrial effluents (such as textile, paper, and leather industries) in water bodies is a significant source of pollution, posing ecological risks that necessitate their removal. The present study demonstrated that the mangrove <em>Sonneratia ovata</em> leaf extract is a promising resource for the biosynthesis of zinc oxide nanoparticles (ZnO NPs), which have potential applications in biomedical fields and wastewater remediation. X-ray diffraction analysis revealed that these nanoparticles have a hexagonal wurtzite structure. Optical measurements indicated the presence of a band gap at 3.19 eV. Morphological studies indicated a mixture of rod-shaped and small spherical nanoparticles. The observed physicochemical properties of the green-synthesized ZnO NPs significantly surpassed the chemically prepared ZnO NPs following a similar synthesis approach. The photocatalytic efficiency of the biosynthesized nanoparticles was tested under UV light irradiation to degrade ethidium bromide dye. This degradation efficiency was shown to be dependent on dye concentration, dye pH, and catalyst concentration. The highest degradation rates of 88.5 %, 98.6 %, and 89.4 % for ethidium bromide dye, respectively, are for dye concentration, dye pH, and nanocatalyst concentration following a first-order kinetic model. Furthermore, the stabilized nanoparticles derived from the green synthesis using <em>S. ovata</em> leaves are potent antioxidants, exhibiting antibacterial activity against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>. These findings highlighted the potential of using natural aqueous extracts from <em>S. ovata</em> to produce phytochemical-enhanced nanoparticles with significant photocatalytic degradation of toxic dyes in water bodies and biomedical applications.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103759"},"PeriodicalIF":3.8,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145105838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of extraction efficiency employing response surface methodology, phytochemical screening, in-vitro antibacterial and antioxidant activity, in-silico studies of tobacco (Nicotiana tabacum) seed oil 利用响应面法优化烟草籽油的提取效率、植物化学筛选、体外抗菌和抗氧化活性、计算机研究

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2025-09-04 DOI: 10.1016/j.bcab.2025.103763

Rutvik D. Joshi , Naved I. Malek , Vimalkumar Prajapati , Bharatkumar Z. Dholakiya

{"title":"Optimization of extraction efficiency employing response surface methodology, phytochemical screening, in-vitro antibacterial and antioxidant activity, in-silico studies of tobacco (Nicotiana tabacum) seed oil","authors":"Rutvik D. Joshi , Naved I. Malek , Vimalkumar Prajapati , Bharatkumar Z. Dholakiya","doi":"10.1016/j.bcab.2025.103763","DOIUrl":"10.1016/j.bcab.2025.103763","url":null,"abstract":"<div><div><em>Nicotiana tabacum</em> seed oil has extensive applications in fuel, cosmetic, and pharmaceutical sectors. This study investigates oil extraction from Bidi tobacco seeds (a variety found in Gujarat, India) using rarely utilized solvents—methylene dichloride (MDC), di-isopropyl ether, and n-butanol—alongside commonly used solvents like n-hexane and petroleum ether. Temperature, extraction time, and solid-solvent ratio were among the parameters that were optimized using a Box-Behnken Design. The solid-solvent ratio of 5.26 g/mL, 5.8 h, and 40 °C produced the maximum oil yield of 10.57 ± 0.04 %. Phytochemicals, including methasterone, linoleic acid, and sclareolide, were detected by GC-MS analysis. Oil extracted from MDC exhibited enhanced physicochemical characteristics. <em>Bacillus cereus</em> was found to be the most sensitive in the antibacterial test, with MICs for <em>S. aureus</em> and <em>E. coli</em> being 450 μg/mL and >500 μg/mL, respectively. At 250 μg/mL, the ABTS assay revealed 73.43 % antioxidant activity. Molecular docking confirmed the strong binding of methasterone to proteins in <em>S. aureus</em> and <em>B. cereus</em>.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103763"},"PeriodicalIF":3.8,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of folate-producing nukadoko by artificial inoculation and investigation of their microbial community 人工接种产叶酸菌菇的制备及其微生物群落调查

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2025-09-03 DOI: 10.1016/j.bcab.2025.103758

Xiaochen Yu, Wenjuan Zhang, Liying Xin, Su Xu, Xiangchen Meng

{"title":"Preparation of folate-producing nukadoko by artificial inoculation and investigation of their microbial community","authors":"Xiaochen Yu, Wenjuan Zhang, Liying Xin, Su Xu, Xiangchen Meng","doi":"10.1016/j.bcab.2025.103758","DOIUrl":"10.1016/j.bcab.2025.103758","url":null,"abstract":"<div><div>In order to increase folate in the nukadoko, the present study was conducted to optimise the fermentation process by artificial inoculation of folate-producing strains of <em>Lactobacillus plantarum</em> Z3 and <em>Pichia myanmarensis</em> J2, and to systematically study the dynamic evolution of microbial communities and their correlation with the physicochemical indexes in the fermentation process. The optimised conditions were as follows: 1:2 (v/v) ratio of <em>Pichia myanmarensis</em> J2 to <em>Lactobacillus plantarum</em> Z3, 6 % inoculum size, 9 % brine concentration, bed thickness of 5–7 cm, fermentation time of 60 days, and addition of 0.1 % ascorbic acid (Vc). Under these optimised conditions, the addition of Vc effectively solved the problem of low folate accumulation rate caused by acidic conditions in the traditional fermentation process. High-throughput sequencing results showed that the diversity of the bacterial community in the nukadoko was higher than that of the fungal community. At the genus level of the bacterial community, <em>Pantoea</em> and <em>Methylobacterium</em> were the dominant genera in the early stage of fermentation (Day0-Day15), and <em>Lactobacillus</em> became the dominant group from Day30 to Day60 with the extension of fermentation time. In contrast, <em>Pichia</em> was always the dominant genus in the fungal community. This study provides a new idea for the development of folate-containing nukadoko, and also lays a preliminary foundation for the preparation of folate-rich nukazuke.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103758"},"PeriodicalIF":3.8,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145020391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of biopolymers by bacterial isolates from a plant biomass-degrading consortium 从植物生物量降解联合体分离的细菌生产生物聚合物

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2025-09-03 DOI: 10.1016/j.bcab.2025.103762

Tatiane Fernanda Leonel , Tereza Cristina Luque Castellane , Guilherme Carvalho Caires , Eliana Gertrudes de Macedo Lemos , Luiz Alberto Colnago , Lucia Maria Carareto-Alves

{"title":"Production of biopolymers by bacterial isolates from a plant biomass-degrading consortium","authors":"Tatiane Fernanda Leonel , Tereza Cristina Luque Castellane , Guilherme Carvalho Caires , Eliana Gertrudes de Macedo Lemos , Luiz Alberto Colnago , Lucia Maria Carareto-Alves","doi":"10.1016/j.bcab.2025.103762","DOIUrl":"10.1016/j.bcab.2025.103762","url":null,"abstract":"<div><div>The present study investigates the potential of bacterial isolates from a plant biomass-degrading consortium for biopolymer production with the use of agroindustrial residues as carbon sources. The investigation focused on the synthesis of exopolysaccharides (EPS), bacterial cellulose (BC), and polyhydroxybutyrate (PHB). Notably, the isolates identified as <em>Agrobacterium</em> sp. and <em>Cohnella</em> sp. successfully produced BC and EPS from lignocellulosic residues. Although PHB production was not detected under the tested conditions, this outcome is likely related to the specific cultivation parameters and metabolic preferences of the isolates. HPLC and FTIR analysis revealed that the produced heteropolysaccharides predominantly consisted of glucose, galactose, and mannose, with low acetate content. The EPS exhibited exceptional emulsification properties, with indices exceeding 70 % when tested with hydrocarbons and used frying oil, both of which are classified as environmental pollutants. These findings highlight the dual benefits of utilizing agro-industrial residues for biopolymer production: addressing waste disposal issues while generating value-added, eco-friendly materials. This study underscores the potential of microbial biopolymer synthesis as a sustainable strategy for waste valorization and industrial innovation.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103762"},"PeriodicalIF":3.8,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome mining of carbohydrate-active enzymes (CAZyme) and poly-γ-glutamic acid (γ-PGA) synthesis by Bacillus velezensis (WA11) directly from lignocellulosic biomass-based substrate 淀粉样芽孢杆菌（Bacillus velezensis, WA11）直接从木质纤维素生物质底物中合成糖活性酶（CAZyme）和聚γ-谷氨酸（γ-PGA）的基因组挖掘

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2025-09-03 DOI: 10.1016/j.bcab.2025.103747

Rakeshkumar Yadav , Pranay Awathare , Pushkar Shitut , Vaishnavi Mahajan , Pranav Nair , Mahesh Dharne

{"title":"Genome mining of carbohydrate-active enzymes (CAZyme) and poly-γ-glutamic acid (γ-PGA) synthesis by Bacillus velezensis (WA11) directly from lignocellulosic biomass-based substrate","authors":"Rakeshkumar Yadav , Pranay Awathare , Pushkar Shitut , Vaishnavi Mahajan , Pranav Nair , Mahesh Dharne","doi":"10.1016/j.bcab.2025.103747","DOIUrl":"10.1016/j.bcab.2025.103747","url":null,"abstract":"<div><div>Poly-γ-glutamic acid (γ-PGA/PGA), a non-toxic and biodegradable polymer with additional diverse properties such as higher heat resistance and water retention, is widely implicated in myriad applications, including agricultural and food processing, medical treatments, and cosmetics. The industrial bio-based production of γ-PGA is primarily hindered by the potential of microbial strains and substrate costs, which are attributed primarily to the carbon sources. The present study is a proof-of-concept study, wherein a bacterial isolate, <em>Bacillus velezensis</em> WA11 strain, was employed for the production of γ-PGA from sugarcane bagasse (lignocellulosic biomass) without any pretreatment or pre-processing. The CAZyme annotation identified several enzyme families involved in metabolizing complex polysaccharides, including cellulose, xylan, and lignin. We obtained 104.3 g/L γ-PGA production with a productivity of 1.09 g/L/h in the optimized synthetic medium containing maltose as a carbon source, 2.9-fold higher than the earlier study using maltose as a carbon source. Further, substituting maltose with untreated sugarcane bagasse resulted in 12 g/L of γ-PGA, higher than most of the reported studies utilizing pretreated lignocellulosic biomass (LCB) lysates for producing γ-PGA. The present study demonstrates the production of γ-PGA using lignocellulosic biomass without pretreatment, providing a chemical-free, sustainable avenue for directly utilizing untreated lignocellulosic biomass (LCB) to produce expensive polymers by employing potential bacterial strains.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103747"},"PeriodicalIF":3.8,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145020383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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