Biocatalysis and agricultural biotechnology最新文献_第3页

Characterization of novel marine bacteria isolated from deep Atlantic Ocean in Morocco as a source of natural bioactive compounds to control infections in aquaculture 从摩洛哥大西洋深海分离的新型海洋细菌的特征，作为控制水产养殖感染的天然生物活性化合物来源

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-09-07 DOI: 10.1016/j.bcab.2024.103350

{"title":"Characterization of novel marine bacteria isolated from deep Atlantic Ocean in Morocco as a source of natural bioactive compounds to control infections in aquaculture","authors":"","doi":"10.1016/j.bcab.2024.103350","DOIUrl":"10.1016/j.bcab.2024.103350","url":null,"abstract":"<div>With the rapid expansion of aquaculture production, there has been a concomitant increase in fish diseases, generally combatted by the utilization of chemicals and antibiotics. However, their overuse leads to the emergence of drug-resistant microorganisms and the appearance of oxidative stress. Consequently, antibiotics contribute to a reduction in fish immune defense. In this work, eight marine bacteria were characterized using physiological, biochemical and microbiological tests, and were evaluated for their safety by studying their haemolytic and gelatinase activities. Then, we evaluated their ability to produce active biomolecules and tested their in vitro antibacterial activities against five bacterial pathogens that are highly prevalent in aquaculture. Moreover, we evaluated their antioxidant potential using DPPH, ABTS and ferric reducing power tests. The obtained results revealed that cell-free supernatants were effective, they exhibited good antioxidant and antibacterial activities. Their biomolecules showed good stability under different temperatures ranging from 60 to 121 °C and were found to be chemically composed of peptides, exopolysaccharides and polyphenols. Therefore, the secreted bioactive substances are of great biotechnological interest and constitute a promising eco-friendly alternative to antibiotics and chemical agents in the context of sustainable aquaculture.</div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142167925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alleviation of salt stress on Zea mays L. plant by PGPR isolates as an effective sustainable strategy 将 PGPR 分离物作为一种有效的可持续战略减轻玉米植物的盐胁迫

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-09-07 DOI: 10.1016/j.bcab.2024.103346

{"title":"Alleviation of salt stress on Zea mays L. plant by PGPR isolates as an effective sustainable strategy","authors":"","doi":"10.1016/j.bcab.2024.103346","DOIUrl":"10.1016/j.bcab.2024.103346","url":null,"abstract":"<div>Among the major constraints for the production of economic crops, such as maize, is salinity. Plenty of research has suggested the use of various strains of plant growth-promoting rhizobacteria (PGPR) as a sustainable approach to improve plant tolerance to salinity. Nonetheless, the exploration of the rhizosphere in Egyptian agricultural lands with salt affected soil remains incomplete. In this research, two newly isolated local bacterial species were finally selected and identified as Alcaligenes sp. strains. E1 (BS-45) and E2 (BS-57). IAA production by both isolates was confirmed by TLC. Several bioactive metabolites were identified in Alcaligenes sp. E1 and E2 by gas chromatography-mass spectroscopy (GC-MS). These include 9,12,15-octadecatrienoic acid, 2,3-bis [(trimethylsilyl)oxy] propyl ester, (z,z,z), 2 (1H)-Naphthalenone, octahydro-1-methyl-1-(2-propenyl)-, (1à,4aá,8aà), dodecane and isopulegol which have a defensive role against biotic and abiotic stress of the plant. Interestingly, the selected bacteria Alcaligenes sp. E1 and E2 significantly improve the overall maize growth attributes, antioxidant scavenging activities and photosynthetic pigments in maize plants grown under salt stress (150 mM NaCl) compared to the control plants as analyzed by greenhouse experiment. Therefore, this study recommends the Alcaligenes sp. strains (E1 and E2) to alleviate the negative impact of salinity on the growth and development of maize seedlings.</div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142239865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Entrapment of glucose oxidase from Aspergillus niger ISL-09 in poly(acrylamide-co-acrylic acid) hydrogels for improved stability and catalytic efficiency towards industrial applications 将黑曲霉的葡萄糖氧化酶 ISL-09 包封在聚（丙烯酰胺-丙烯酸共聚物）水凝胶中以提高稳定性和催化效率，从而实现工业应用

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-09-06 DOI: 10.1016/j.bcab.2024.103349

{"title":"Entrapment of glucose oxidase from Aspergillus niger ISL-09 in poly(acrylamide-co-acrylic acid) hydrogels for improved stability and catalytic efficiency towards industrial applications","authors":"","doi":"10.1016/j.bcab.2024.103349","DOIUrl":"10.1016/j.bcab.2024.103349","url":null,"abstract":"<div>The present study highlights the true potential of Aspergillus niger ISL-09 to produce glucose oxidase (GOx) and focuses on improving the catalytic properties of GOx by entrapping it in poly acrylamide-co-acrylic acid (AAm-co-AAc) hydrogels, fabricated by free-radical polymerization method. For this purpose, the optimum conditions for GOx activity were found to be: 15 g soybean meal (SBM) substrate, 20 mL moisture content, 96 h incubation period, and 1.5 mL of fungal spore suspension. The purified extracellular GOx was observed to have 32.4% yield, with 0.02 U/mg specific activity and approximately 80 kDa subunit molecular weight. The GOx was most active at pH 5.5 and 40 °C. The immobilization of GOx in poly (AAm-co-AAc) hydrogels led towards improved stability and catalytic efficiency, resulting in a 21.7% increase in activity compared to free enzyme. The study also examined the potential of GOx in the pharmaceutical and textile industries as Ca-gluconate producer and bleaching agent, respectively. The study concludes that A. niger ISL-09 is a promising source for GOx production under optimal conditions. Furthermore, the immobilization of GOx in poly (AAm-co-AAc) hydrogels can significantly improve its catalytic properties, making it suitable for different industrial applications. However, further scaling up is required for the better implementation in industry.</div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142158290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the antibacterial efficacy of Satureja hortensis and Bacillus subtilis extracts in combating Xanthomonas citri 探索山苍子和枯草芽孢杆菌提取物在防治柠檬黄单胞菌中的抗菌功效

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-09-05 DOI: 10.1016/j.bcab.2024.103348

{"title":"Exploring the antibacterial efficacy of Satureja hortensis and Bacillus subtilis extracts in combating Xanthomonas citri","authors":"","doi":"10.1016/j.bcab.2024.103348","DOIUrl":"10.1016/j.bcab.2024.103348","url":null,"abstract":"<div>Biofilm plays a critical role in protecting the associated bacterial colonies. Citrus canker caused by Xanthomonas citri, resulted in remarkable yield reduction in citrus orchards. This study aims to evaluate the anti-biofilm properties of S. hortensis and B. subtilis extracts against X. citri. The antibacterial effects of both extracts were evaluated using the disk diffusion method and 96-well microdilution plates. Crystal violet and XTT procedures were employed to assess the inhibition of adhesion and antibiofilm effects of both extracts. The checkboard titration method was applied to determine the synergistic effects of the plant-bacterial extracts. The antibiofilm effects were confirmed by the light microscopy method. The results showed that the antibacterial and antibiofilm properties of both extracts varied. The MIC values of S. hortensis and B. subtilis extracts were 6.25 and 50 mg/ml, respectively. In these concentrations the inhibition of adhesion effects of S. hortensis and B. subtilis were 70% and 80%, whereas their antibiofilm effects were 60% and 72%. S. hortensis and B. subtilis extracts showed 63% and 76% antibiofilm activity, respectively, using the XTT test. The extracts showed synergistic effects, resulting in higher inhibition rates in combinatorial applications. Molecular docking results also confirmed that the secondary metabolites of S. hortensis could interact with different X. citri proteins differentially. Further large-scale studies on combinatorial antibiofilm effects of plant and bacterial extracts against citrus pathogens will provide new opportunities to develop safe and applicable reagents to control these destructive diseases.</div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142240588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New tools for effective production and long-term stabilization of microcystinase (MlrA) - A biotechnological perspective towards hepatotoxic microcystins remediation 有效生产和长期稳定微囊藻毒素酶（MlrA）的新工具--从生物技术角度解决肝毒性微囊藻毒素问题

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-09-05 DOI: 10.1016/j.bcab.2024.103347

{"title":"New tools for effective production and long-term stabilization of microcystinase (MlrA) - A biotechnological perspective towards hepatotoxic microcystins remediation","authors":"","doi":"10.1016/j.bcab.2024.103347","DOIUrl":"10.1016/j.bcab.2024.103347","url":null,"abstract":"<div>Microcystinase (MlrA) is the most specific catalyst and the most efficient enzyme of known microcystins (MCs) detoxification pathways. Very recently, direct MlrA application has effectively degraded MCs within industrial processes, demonstrating MlrA application for sustainable in situ MCs remediation. Heterologous MlrA expression in cyanobacteria offers a unique opportunity, linking harmful MCs remediation with emerging cyanobacterial biotechnologies. Here, we first generate a novel Synechocystis sp. PCC 6803 (6803) using non-native trc promoter for MlrA expression. Whole-cell MlrA activity was comparable to previously described expression via native 6803 PcpcB560 promoter, while cellular extracts of the new strain showed significantly higher MlrA yields (2–15 times, depending on the age of the cultures). Furthermore, efficiency of MlrA production under multiple photoautotrophic cultivation conditions varied, but was not improved by Na2CO3 supplementation nor under increased light, indicating the need to explore new photoautotrophic chassis for higher MlrA productivity.Methods for MlrA stabilization are critical for industrial development, thus lyophilization of MlrA-enriched cellular extracts was explored. Recovered MlrA activity was not statistically different from initial MlrA activity following storage of lyophilized extracts at −20 °C for 20 weeks. In contrast, storage at 20 °C and storage of aqueous lysates at 4 °C resulted in progressive MlrA activity loss. Such stabilized lyophilizate may offer a checkpoint for further optimization of upstream processes (production), while expanding potential for downstream investigations (application), contributing simultaneously to novel MlrA-based MCs remediation approaches and to cyanobacterial biotechnology.</div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1878818124003311/pdfft?md5=66702ef9e23e308eb8b9e51bb391bd10&pid=1-s2.0-S1878818124003311-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142161625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current and prognostic overview of digestate management and processing practices, regulations and standards 沼渣管理和处理方法、法规和标准的现状和前景概述

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-09-05 DOI: 10.1016/j.bcab.2024.103345

{"title":"Current and prognostic overview of digestate management and processing practices, regulations and standards","authors":"","doi":"10.1016/j.bcab.2024.103345","DOIUrl":"10.1016/j.bcab.2024.103345","url":null,"abstract":"<div>Digestate is a byproduct of anaerobic digestion (AD) where organic waste is converted into biogas, a clean source of energy. The growing interest in renewable energy and waste management has increased the number of AD plants worldwide in recent years. Therefore, the appropriate digestate management has shifted towards the concept of waste to products and the realization of a circular economy to maximize the benefits of AD plants as well as digestate valorization. The various properties of digestate such as organic matter, nutrient composition, moisture content, and plausible pollutants, determine its suitability for further application. Proper management and handling are required to minimize environmental risks and enhance the value of the digestate valorization. Instead, its use in conventional ways, novel strategies for digestate utilization are emerging to properly benefit and practically realize the biorefinery of AD plants at a large scale. Moreover, the proper knowledge of regulations and standards concerning the proper digestate utilization is required. This article summarizes the digestate properties and their factor affecting, digestate treatment technologies. The current digestate valorization practices have been discussed along with the focus on the novel and emerging practices. Most importantly, the regulations and standards followed around the globe have been majorly focused upon so that the environmental concerns arising due to its utilization should be taken care of well. Further, the challenges and future perspectives of digestate management and valorization are discussed to be understood for attaining a full circular economic concept in the AD plants at an industrial scale.</div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142171823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous expression of GH11 xylanase from Myceliophthora heterothallica F.2.1.4 in Pichia pastoris 在 Pichia pastoris 中异源表达来自 Myceliophthora heterothallica F.2.1.4 的 GH11 木聚糖酶

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-09-04 DOI: 10.1016/j.bcab.2024.103343

{"title":"Heterologous expression of GH11 xylanase from Myceliophthora heterothallica F.2.1.4 in Pichia pastoris","authors":"","doi":"10.1016/j.bcab.2024.103343","DOIUrl":"10.1016/j.bcab.2024.103343","url":null,"abstract":"<div>This research is centered on improving a xylanase enzyme derived from Myceliophthora heterothallica by expressing it in Pichia pastoris to enhance biomass hydrolysis. Lignocellulosic biomass holds significant potential for renewable applications in biofuels, chemicals, and pharmaceuticals. The study explores the impact of the choice of expression host on enzyme properties, specifically addressing challenges in thermostability by utilizing P. pastoris and leveraging its glycosylation capabilities. Previously expressed in Escherichia coli, the xylanase exhibited in this work cooperative kinetics, pH stability, and resistance to phenolic compounds. Gene integration and expression in P. pastoris were verified through PCR and activity assays. After 120 h of induction, an enzymatic activity of 48.8 U mL−1 was obtained. Subsequent characterization revealed improved specific activity, substrate affinity, and optimal temperature compared to the enzyme expressed in E. coli. The enzyme exhibited excellent pH and temperature stability for industrial applications, maintaining over 90% of its activity within a pH range of 5.0–10.0 and remaining stable even after 90 min of incubation at 55 °C. It also demonstrated resistance to metal ions and responsiveness to phenolic compounds. These findings underscore the versatility of the recombinant xylanase from M. heterothallica expressed in P. pastoris, highlighting its potential as a valuable resource for biomass conversion. The study emphasizes the pivotal role of host choice in optimizing enzyme characteristics for industrial applications, underscoring the importance of employing heterologous expression systems, as demonstrated in this investigation.</div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142148081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “ Preliminary study for evaluation of some fungicides against Aspergillus flavus isolated from historical illuminated paper manuscript dated back to the Mamluk period” [Biocatal. Agric. Biotechnol., 57 (2024) 103113] 对 "针对从马木留克时期历史性纸质手稿中分离出的黄曲霉菌的一些杀菌剂的初步评估研究 "的更正[Biocatal. Agric. Biotechnol., 57 (2024) 103113]

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-08-10 DOI: 10.1016/j.bcab.2024.103342

引用次数: 0

Optimization of process parameter and quality evaluation of tray dried bori: A traditional Indian nugget 托盘烘干 Bori 的工艺参数优化和质量评估：一种传统的印度金块

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-08-06 DOI: 10.1016/j.bcab.2024.103334

引用次数: 0

Xylanase from Neobacillus sedimentimangrovi UE25: Characterization, purification and applications 来自沉积新杆菌 UE25 的木聚糖酶：表征、纯化和应用

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-08-06 DOI: 10.1016/j.bcab.2024.103331

{"title":"Xylanase from Neobacillus sedimentimangrovi UE25: Characterization, purification and applications","authors":"","doi":"10.1016/j.bcab.2024.103331","DOIUrl":"10.1016/j.bcab.2024.103331","url":null,"abstract":"<div>Considering various industrial applications of thermostable xylanases and scarcity of data available on xylan degradation potential of the Neobacillus species, this study was designed to characterize xylanase produced by the thermophilic strain of N. sedimentimangrovi UE25 using sugarcane bagasse as a carbon source. Xylanase was characterized by adopting a Central Composite design (CCD), which has not been reported earlier for this purpose. The enzyme exhibited thermal stability over a range of temperatures from 50 to 80 °C and its optimal activity was obtained at 65 °C and pH 4.0. The enzyme did not lose its activity for approximately 13 days when kept at 4 °C and retained 84% of its catalytic activity even after 2.5 h of incubation at 65 °C. Various metals and chemicals resulted in enhanced xylanase activity when mixed with this enzyme. Moreover, xylanase was purified by ion exchange chromatography, and its molecular weight was estimated to be ∼60 kDa. The purified xylanase was applied in a saccharification process to improve the total phenolic content (90 mg GAE/g) of the biomass of a halophytic plant, Ipomoea pes-caprae.</div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141963129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0