Biocatalysis and agricultural biotechnology最新文献

{"title":"Study on the mechanism of mixed biochar enhancing methane production in anaerobic digestion of corn straw","authors":"Huilin Zhang ,&nbsp;Siyu Zhang ,&nbsp;Pujie Wei ,&nbsp;Xinyue Zhao ,&nbsp;Hong Chang ,&nbsp;Zeyan Mu ,&nbsp;Xu Zhang ,&nbsp;Wei Liu ,&nbsp;Xudan Liu ,&nbsp;Xiaohong Su ,&nbsp;Xin Wang","doi":"10.1016/j.bcab.2026.104027","DOIUrl":"10.1016/j.bcab.2026.104027","url":null,"abstract":"<div><div>China has a large annual output of straw but a low utilization rate. Anaerobic digestion is an effective way to achieve its energy utilization, and biochar can enhance the efficiency of this process. This study focuses on the mechanism by which mixed biochar regulates microbial communities and promotes methane production and straw degradation during anaerobic digestion. The results show that when the ratio of corn straw biochar to hemp stalk biochar is 4/6 and the total addition amount is 10%, the cumulative methane production reaches 273.15 mL/gVS, which is 58.69% higher than that of the blank group. Through SEM and XRD characterization, it can be seen that biochar can reduce the crystallinity of cellulose in corn straw and destroy its structure. This structural change provides microorganisms with more easily utilized substrates; at the same time, biochar can provide attachment sites for microorganisms, promote electron transfer, and maintain the diversity and abundance of bacterial, fungal, and archaeal communities. According to KEGG metabolic pathway analysis, biochar can further enhance the overall metabolic activity of archaea and improve their metabolic efficiency. The optimized structure and activity of the dominant microbial communities further guarantee high anaerobic digestion efficiency, providing key technical support for the engineering application of straw anaerobic digestion.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"73 ","pages":"Article 104027"},"PeriodicalIF":3.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147612258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cow blood hydrolysis using pineapple waste: Optimization for amino acid release and potential antioxidant bioactive production","authors":"Mojtaba Zarea ,&nbsp;Noori M. Cata Saady ,&nbsp;Sohrab Zendehboudi","doi":"10.1016/j.bcab.2026.103958","DOIUrl":"10.1016/j.bcab.2026.103958","url":null,"abstract":"<div><div>Enzymatic hydrolysis of slaughterhouse blood is constrained by the high cost and limited availability of purified enzymes, hindering large-scale waste valorization. This study aims to develop a low-cost and sustainable hydrolysis strategy by directly utilizing raw pineapple waste (PW) as a natural protease source for cow blood (CB), without enzyme purification or supplementation. A central composite design (CCD) was employed to optimize hydrolysis time, PW concentration, and CB concentration, while protein reduction (P_red), free amino acid (FAA) release, and volatile solids reduction (VS_red) were evaluated as key responses. The developed models were statistically significant (p &lt; 0.0001) with R² values exceeding 0.90, demonstrating strong predictive reliability. Under optimized conditions (desirability = 0.98), P_red and VS_red reached 96% and 30%, respectively, at PW:CB concentrations of 10:4 g VS L⁻¹, while FAA concentration increased to 1321 μg mL⁻¹ at PW:CB concentration of 10:8 g VS L⁻¹ after 72 h. Extending the hydrolysis period to 168 h further increased FAA concentration to 2731 μg mL⁻¹ and enhanced antioxidant activity by 4.2-fold, achieving 51% 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. Fourier-transform infrared spectroscopy (FTIR) analysis confirmed peptide bond cleavage and disruption of protein secondary structures, indicating the formation of low-molecular-weight peptides. Compared to purified enzyme approaches, this study demonstrates, for the first time, the direct waste-to-waste use of untreated PW as the sole protease source for CB hydrolysis, eliminating enzyme processing costs. The process provides an eco-friendly and cost-effective pathway for CB valorization, supporting bioenergy applications, specifically anaerobic digestion and antioxidant-rich hydrolysate production for food, nutraceutical, and pharmaceutical industries.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"73 ","pages":"Article 103958"},"PeriodicalIF":3.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146186892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The light side of Fusarium as an added-value enzymes producer","authors":"Esmeralda Martínez-Torres ,&nbsp;Javier Plasencia ,&nbsp;Carmina Montiel","doi":"10.1016/j.bcab.2026.103956","DOIUrl":"10.1016/j.bcab.2026.103956","url":null,"abstract":"<div><div>The fungal genus <em>Fusarium</em> comprises many pathogenic species responsible for various plant diseases, including agave and tomato wilt, corn rot, and wheat head blight. These maladies have a significant impact on crop productivity, food security, and public health, resulting in substantial economic losses due to reduced yields and commodity quality.</div><div><em>Fusarium</em> spp. produce a wide range of enzymes, mainly hydrolases, such as cellulases, pectinases, xylanases, amylases, and inulinases, which degrade the plant cell walls and stored carbohydrates. These enzymes hold significant potential across various industries for the production of biofuels, food, beverages, and pharmaceuticals due to their ability to break down complex plant materials. Therefore, <em>Fusarium</em> spp. may have a lighter side as a source of enzymes with novel properties and valuable industrial applications, which are worth studying.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"73 ","pages":"Article 103956"},"PeriodicalIF":3.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146187147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA-seq reveals altitude driven metabolic reprogramming for the enhanced stress tolerance in mungbean (Vigna radiata L. Wilczek) microgreens","authors":"Gyan P. Mishra ,&nbsp;Muralidhar Aski ,&nbsp;K.M. Shivaprasad ,&nbsp;Tsering Stobdan ,&nbsp;Shiksha Chaurasia ,&nbsp;Manju Kohli ,&nbsp;Ajeet Singh Dhaka ,&nbsp;Ranjeet R. Kumar ,&nbsp;Rekha Kumari Meena ,&nbsp;Soma Gupta ,&nbsp;Suneha Goswami ,&nbsp;T. Vinutha ,&nbsp;Ramakrishnan M. Nair ,&nbsp;Harsh K. Dikshit","doi":"10.1016/j.bcab.2026.104030","DOIUrl":"10.1016/j.bcab.2026.104030","url":null,"abstract":"<div><div>Microgreens are globally recognized as nutrient-dense, functional foods; however, the molecular basis of their environmental nutritional plasticity remains largely unexplored. Thus, RNA sequencing (RNA-Seq) was employed to elucidate genes and pathways underlying differential expression in mungbean (cv. MH810) microgreens grown under different altitudes. The analysis generated 367.93 million high-quality reads (HQRs) with up to 95.41% alignment efficiency and identified 15,135 differentially expressed genes among 29,282 annotated genes. Gene set enrichment analysis revealed a reallocation of resources in Leh-grown microgreens from cell division and structural polysaccharide production toward enhanced vitamin metabolism, antioxidant capacity, and stress pathways compared to normal growing conditions. Weighted gene co-expression network analysis revealed functional overlap between secondary metabolism and phenylpropanoid biosynthesis. MicroRNA profiling identified three key miRNAs (MIR159, MIR169_2, and MIR171_1) associated with stress-responsive and developmental pathways, having their role in high-altitude (Leh) based physiological adaptation. Major transcription factor families include bHLH, AP2/ERF, WRKY, NAC, and TIFY, while PPI modules formed coherent functional complexes, supporting the reprogramming of Leh-grown microgreens from growth-centric processes toward stress acclimation. Additionally, 506 novel EST-SSR (Expressed Sequence Tag-Simple Sequence Repeat) markers spanning all 11 chromosomes were developed, providing valuable genomic resources for trait-linked marker development and breeding. In silico validation highlighted key stress- and growth-related genes, including JAZ6, pectin acetylesterases, GDSL-like lipase, peroxidases, and bHLH and TIFY transcription factors. This maiden RNA-Seq study demonstrates that the Leh conditions reprogram microgreens from active growth and cell-wall deposition toward genome maintenance and enhanced stress signaling.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"73 ","pages":"Article 104030"},"PeriodicalIF":3.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147612186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marine algae-derived polyamines and phlorotannins as cholinesterase inhibitors: In vitro and in silico analysis","authors":"Adem Necip ,&nbsp;Mesut Işık ,&nbsp;Cüneyt Türkeş ,&nbsp;Dilek Ünal ,&nbsp;Kevser Kübra Kırboğa ,&nbsp;Şükrü Beydemir ,&nbsp;Mithun Rudrapal","doi":"10.1016/j.bcab.2026.104035","DOIUrl":"10.1016/j.bcab.2026.104035","url":null,"abstract":"<div><div>The limitations of conventional cholinesterase inhibitors in Alzheimer's disease (AD) therapy namely, their short half-lives and adverse side effects—necessitate the exploration of natural alternatives. This investigation examined the inhibitory potential of selected polyamines (putrescine and spermidine) and a phlorotannin derivative (phloroglucinol), naturally present in the macroalgae species <em>Cystoseira crinita</em> and <em>Stypopodium schimperi</em>, against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) using <em>in vitro</em> and in silico approaches. Additionally, radical scavenging potentials of each sample were determined. Enzyme activity assays employing the Ellman method revealed that putrescine and spermidine reached similar inhibition levels (approximately 32 %), while <em>Cystoseira crinita</em> acetone extract demonstrated superior potency with an IC50 of 0.085 mg/mL, indicating robust enzyme modulation. Complementary molecular docking studies conducted with Schrödinger Maestro 2020 elucidated the formation of stable enzyme-ligand complexes through hydrogen bonding and π-π interactions with critical residues including HIS447, TYR337, and TRP86 within the AChE active site. Moreover, crude macroalgal extracts, particularly those derived from <em>C. crinita</em> and <em>S. schimperi</em>, exhibited marked AChE inhibition, further substantiating the therapeutic potential of these marine-derived compounds. Collectively, these findings demonstrate that the polyamines and phlorotannin derivative studied not only effectively regulate cholinesterase activity but also offer naturally derived structural frameworks for the development of novel AD treatments. Phloroglucinol showed a high scavenging of DPPH (85%) and ABTS (98%) radicals, close to the activity of the reference compound trolox (96% for DPPH and 94% for ABTS). Further pharmacokinetic and toxicological evaluations are warranted to ascertain their clinical viability in AD management.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"73 ","pages":"Article 104035"},"PeriodicalIF":3.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147656341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastic exposure and its impact on the growth and physiological functions of Cicer arietinum","authors":"Garima Kaushik,&nbsp;P. Manuraj,&nbsp;Mohd Ashraf Dar,&nbsp;Preksha Palsania,&nbsp;Shalni Satya,&nbsp;Ommer Ahad Bhat,&nbsp;Shailesh Kumar Patidar","doi":"10.1016/j.bcab.2026.103920","DOIUrl":"10.1016/j.bcab.2026.103920","url":null,"abstract":"<div><div>The ubiquity of plastic waste pollution, a hallmark of the Anthropocene, leads to microplastic (MP) contamination in soil, posing risks to plant health by significantly impairing overall growth and reduced vegetative development. This study explores the effects of varying polyethylene terephthalate (PET) MP concentrations (1 %, 2 %, and 4 % w/w) on the growth and cellular function of <em>Cicer arietinum</em>. Results indicate a dose-dependent decline in seed germination, seedling vigor index, and cumulative germination energy, suggesting compromised early plant development. A significant reduction in biomass was observed at higher MP concentrations, with plant dry weight decreasing from 23.91 mg in the control to 11.24 mg at 4 % MP exposure. In contrast, leaf area and width remained less affected, implying selective growth responses under stress. MP exposure was associated with signs of impaired photosynthesis, as evidenced by a sharp reduction in total chlorophyll content from 64.5 mg/L in the control to 17.6 mg/L in the 1 % MP treatment. Cellular health markers also declined; dehydrogenase activity was diminished in all MP treatments, and exposure was linked to severe membrane injury, with relative cell injury increasing from 65.2 % in the control to 95.1 % in the 4 % MP treatment. Notably, ascorbic acid levels increased from 4.66 μg/g to 6.59 μg/g with higher MP concentrations, suggesting an active defensive stress response. This study documents a suite of negative physiological effects associated with PET MP exposure, providing a quantitative basis for further research into MP toxicity and crop resilience.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103920"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145941031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of chitosanases of Lecanicillium lecanii in pilot-scale submerged cultures for production of low molecular weight chitosan and chitooligosaccharides","authors":"Jennyfer Hernández-Alcántara ,&nbsp;Jesús Rojas-Osnaya ,&nbsp;Makoto Anraku ,&nbsp;Hideaki Nakamura ,&nbsp;Keiko Shirai","doi":"10.1016/j.bcab.2026.103927","DOIUrl":"10.1016/j.bcab.2026.103927","url":null,"abstract":"<div><div>Chitosan with a degree of acetylation of 18.15 % at concentrations of 5, 10, and 15 g L⁻¹ was used as an inducer and sole carbon source in Czapek medium for chitosanase production by <em>Lecanicillium lecanii</em> in 3 L bioreactor submerged cultures. Subsequently, chitosanase production was scaled up to 50 L in submerged fermentation using the selected chitosan concentration (10 g L⁻¹) added to the culture medium, while maintaining key operational parameters, such as pH, temperature, and volumetric oxygen mass transfer coefficient. The enzymes were purified by salting-out, molecular size exclusion, and anionic exchange chromatography. The chitosanases presented molecular weights of 31 and 44 kDa, with optimal reaction conditions of 50 °C and pH 6, yielding a specific chitosanase activity of 25.97 ± 0.43 U mg⁻¹. This activity enabled the enzymes to cleave substrates such as glycol chitosan, chitosan (degree of acetylation 18.15 %), and 4-methylumbelliferyl-<em>N</em>-acetyl-β-<em>D</em>-glucosamine. The chitosanases produced chitooligomers with 2 and 6 repeating units, as well as low molecular weight chitosan with molecular masses ranging from 8 to 102 kDa, and an increased degree of acetylation in COS. The degree of hydrolysis was controlled by the chitosanase concentration (purity grade) and the reaction time, allowing the production of chitosan derivatives on a pilot scale. The employment of chitosanases facilitates the production of partially acetylated oligosaccharides and low-molecular-weight chitosan, which has broad potential in industrial applications, including pharmaceuticals, agriculture, and food technology.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103927"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The promising effects of bacterial and microalgal inoculants in enhancing tomato growth and improving soil quality","authors":"Tongtong Xu ,&nbsp;Qixiang Xian ,&nbsp;Yunqian Cui ,&nbsp;Huali Pei ,&nbsp;Wei Liu ,&nbsp;Mingjun Chen ,&nbsp;Jingling Liu ,&nbsp;Hongbo Yu ,&nbsp;Nortoji A. Khujamshukurov ,&nbsp;Meimei Wu","doi":"10.1016/j.bcab.2026.103937","DOIUrl":"10.1016/j.bcab.2026.103937","url":null,"abstract":"<div><div>The overuse of chemical fertilizers threatens soil health and sustainable agriculture. While bacterial biofertilizers are widely used, microalgae offer a promising alternative due to their low cost and accessibility. This study investigated the effects of bacterial and microalgal inoculants on tomato (<em>Solanum lycopersicum</em> L.) growth, fruit quality, and soil properties through a 100-day pot experiment. The results showed that the application of composite bacteria inoculant and microalgae inoculant significantly promoted tomato growth, increased soil nutrient levels, and improved tomato yield and fruit quality. At 100 days post-inoculation, the T4 group (60 mL combined bacterial inoculant) exhibited the most pronounced growth-promoting effects on tomato plants. Compared with the T1 group (soil not inoculated with bacteria/microalgae), the T4 group significantly enhanced plant height (13.8 %), leaf number (48.3 %), leaf dimensions (19.7 % and 31.2 % in length and width, respectively), and stem diameter (11.3 %). Regarding fruit quality, the T4 group yielded tomatoes with the highest vitamin C content (23.52 ± 0.30 mg·100 g⁻¹). Notably, among all microalgae treatments, the T8 group (<em>Tribonema</em> sp.) demonstrated optimal performance, showing significantly higher contents of both fructose (3.62 ± 0.13 mg g⁻¹) and titratable acid (0.29 ± 0.03 %) in fruits compared to other groups. Actinobacteriota and Proteobacteria were the dominant phyla in all soil groups. This study contributes to the systematic evaluation of the promoting effects and application prospects of composite bacteria inoculant and microalgae inoculant for enhancing crop growth, yield, and soil amelioration.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103937"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salt stress mitigation in pomegranate using olive mill solid waste derived phenolic extract","authors":"Sahar Ben Abdelwaheb ,&nbsp;Samia Abboud ,&nbsp;Azhar Ouni ,&nbsp;Darine Tlili ,&nbsp;Mourad Jellali ,&nbsp;Amani Bchir ,&nbsp;Noomene Sleimi ,&nbsp;Soumaya Dbara","doi":"10.1016/j.bcab.2026.103943","DOIUrl":"10.1016/j.bcab.2026.103943","url":null,"abstract":"<div><div>Salinity is a major abiotic stress limiting pomegranate productivity, and sustainable mitigation strategies are needed. This study evaluated the potential of a phenolic extract (PE) derived from olive mill solid waste (OMSW) to alleviate salt stress in two Tunisian pomegranate cultivars, Kalai and Testouri. The extract is rich in bioactive phenolic compounds, mainly quinic acid. Two-year-old plants were grown under semi-controlled greenhouse conditions from August to October 2023 and irrigated with 0,150 (≈18 dS m⁻¹), and 200 mM NaCl (≈22.5 dS m⁻¹). Plants were foliar-sprayed weekly with 300 ppm PE, while untreated plants served as controls. Salt stress reduced vegetative growth, decreased chlorophyll content and effective quantum yield of photosystem II (ΦPSII), and increased oxidative stress. In <strong>Kalai</strong>, PE enhanced growth and pigment content, notably increasing chlorophyll <em>a</em> and carotenoids under 200 mM NaCl and chlorophyll <em>b</em> under 150 mM NaCl. These effects were accompanied by an improvement in ΦPSII, a reduction in oxidative damage (lower malondialdehyde (MDA) and reduced hydrogen peroxide (H₂O₂) at 150 mM NaCl), although electrolyte leakage increased. In Testouri, PE promoted growth and pigment accumulation mainly under 150 mM NaCl. While ΦPSII was not significantly affected, PE reduced oxidative stress by lowering H₂O₂ at 150 mM NaCl and MDA at 200 mM NaCl, and improved membrane stability by decreasing electrolyte leakage under both 150 and 200 mM NaCl. In both cultivars, PE reduced Na ⁺ accumulation and improved K⁺ content under 200 mM NaCl, indicating enhanced ion homeostasis. However, under non-saline conditions, PE induced mild stress-related responses in both cultivars. Overall, these findings indicate that OMSW-derived PE exerts its beneficial effects mainly under salt stress.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103943"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cytotoxic potential of water-methanol-extracted secondary metabolites from lichens as an important source for treating human cancer cell lines","authors":"Łukasz Furmanek ,&nbsp;Natalia Żurek ,&nbsp;Ireneusz Kapusta ,&nbsp;Mark R.D. Seaward ,&nbsp;Paweł Czarnota","doi":"10.1016/j.bcab.2026.103934","DOIUrl":"10.1016/j.bcab.2026.103934","url":null,"abstract":"<div><div>The results of <em>in vitro</em> experiments on the cytotoxic potential of water-methanol extracts obtained from 11 lichen species, <em>Cetraria islandica</em>, <em>Cladonia arbuscula</em>, <em>Cladonia digitata</em>, <em>Cladonia gracilis</em>, <em>Cladonia phyllophora</em>, <em>Cladonia rangiferina</em>, <em>Cladonia uncialis</em>, <em>Hypogymnia physodes</em>, <em>Platismatia glauca</em>, <em>Pseudevernia furfuracea</em> and <em>Usnea dasopoga</em>, against five human cancer cell lines - AGS, Dld-1, U251mg, LS180 and Ht-29 are presented. The antioxidant potential of the extracts was determined by means of the DPPH˙, FRAP and chelating ability of metals ion (ChP) tests. The highest activity for DPPH˙ (225.33 mmol TE/100 g d.m.) and FRAP (229.02 mmol TE/100 g d.m.) tests were shown for <em>C. digitata</em> extract, while for the ChP test, the extracts obtained from <em>C. digitata</em> and <em>H. physodes</em> species (IC₅₀: 2.10 mg/mL). For the cytotoxicity test, the strongest potential was shown for the <em>C. digitata</em> extract against all the tested cancer cell lines - AGS (IC₅₀: 123.14 μg/mL), Dld-1 (113.20 μg/mL), U251mg (137.20 μg/mL), LS180 (125.67 μg/mL) and Ht-29 (111.64 μg/mL). Slightly weaker cytotoxic efficacy was shown by extracts obtained from <em>H. physodes</em> and <em>P. furfuracea</em>. The qualitative-quantitative analysis of the profile of extracted lichen secondary metabolites performed by UPLC-Q-TOF-MS/MS identified 30 secondary compounds, the most abundant of which were fumarprotocetraric acid, physodic acid isomer, 3-hydroxyphysodic acid and usnic acid. The cytotoxicity potential of the tested extracts against AGS, DLD-1 and LS180 cell lines has been performed as a first approach worldwide. The results of all tests and analyses showed research opportunities in phytotherapy in the future.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103934"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}