Production of biopolymers by bacterial isolates from a plant biomass-degrading consortium

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology Pub Date : 2025-09-03 DOI:10.1016/j.bcab.2025.103762

Tatiane Fernanda Leonel , Tereza Cristina Luque Castellane , Guilherme Carvalho Caires , Eliana Gertrudes de Macedo Lemos , Luiz Alberto Colnago , Lucia Maria Carareto-Alves

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引用次数: 0

Abstract

The present study investigates the potential of bacterial isolates from a plant biomass-degrading consortium for biopolymer production with the use of agroindustrial residues as carbon sources. The investigation focused on the synthesis of exopolysaccharides (EPS), bacterial cellulose (BC), and polyhydroxybutyrate (PHB). Notably, the isolates identified as Agrobacterium sp. and Cohnella sp. successfully produced BC and EPS from lignocellulosic residues. Although PHB production was not detected under the tested conditions, this outcome is likely related to the specific cultivation parameters and metabolic preferences of the isolates. HPLC and FTIR analysis revealed that the produced heteropolysaccharides predominantly consisted of glucose, galactose, and mannose, with low acetate content. The EPS exhibited exceptional emulsification properties, with indices exceeding 70 % when tested with hydrocarbons and used frying oil, both of which are classified as environmental pollutants. These findings highlight the dual benefits of utilizing agro-industrial residues for biopolymer production: addressing waste disposal issues while generating value-added, eco-friendly materials. This study underscores the potential of microbial biopolymer synthesis as a sustainable strategy for waste valorization and industrial innovation.

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从植物生物量降解联合体分离的细菌生产生物聚合物

本研究探讨了从植物生物量降解联合体分离的细菌利用农业工业残留物作为碳源生产生物聚合物的潜力。主要研究了胞外多糖（EPS）、细菌纤维素（BC）和聚羟基丁酸酯（PHB）的合成。值得注意的是，鉴定为Agrobacterium sp.和Cohnella sp.的分离株成功地从木质纤维素残留物中产生了BC和EPS。虽然在测试条件下没有检测到PHB的产生，但这一结果可能与菌株的特定培养参数和代谢偏好有关。HPLC和FTIR分析表明，所得杂多糖主要由葡萄糖、半乳糖和甘露糖组成，乙酸含量较低。EPS表现出优异的乳化性能，与碳氢化合物和用过的煎炸油（均为环境污染物）的乳化指数均超过70%。这些发现强调了利用农业工业残留物生产生物聚合物的双重好处：解决废物处理问题，同时产生增值的、环保的材料。这项研究强调了微生物生物聚合物合成作为废物增值和工业创新的可持续战略的潜力。

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来源期刊

Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

7.70

自引率

2.50%

发文量

308

审稿时长

48 days

期刊介绍： Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.