Biocatalysis and agricultural biotechnology最新文献

{"title":"Evaluation of cytotoxicity of silver nanoparticles on human skin melanoma SK- MEL-3 cell line: In vitro model","authors":"","doi":"10.1016/j.bcab.2024.103333","DOIUrl":"10.1016/j.bcab.2024.103333","url":null,"abstract":"<div><p>Globally the rate of melanoma skin cancer has increased in the recent years. Conventional treatments like chemotherapy, radiotherapy and surgery will damage the cancer cells and some healthy cells also in the body. These treatments are expensive and severe side effects on humans. Nanotechnology have recently been used in biological systems to enhance both reformative and translational therapies. In this study, we used binary solvent for preparation of plant extracts and phytosynthesized AgNPs. It is well known that AgNPs synthesized using green methods are preferred as eco-friendly, low cost, rapid and efficient process. For the assessment of the therapeutic activity of synthesized AgNPs, we proposed an in-vitro model of human melanoma skin cancerous cells (SK-MEL-3). The specific characteristics of AgNPs such as size, shape, distribution, crystalline structure etc. are examined before the medicinal application. In addition, we have done cytotoxicity analysis and apoptotic study to understand the morphological alteration in malignant cells.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141997192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seed extracts as an effective strategy in the control of plant pathogens: Scalable industry bioactive compounds for sustainable agriculture","authors":"","doi":"10.1016/j.bcab.2024.103332","DOIUrl":"10.1016/j.bcab.2024.103332","url":null,"abstract":"<div><p>With a growing global population, maintaining sufficient agricultural production is crucial. However, agriculture faces numerous challenges today, particularly due to the undeniable impacts of climate change, which are expected to intensify pest and disease pressures. The traditional approach to combat these phytopathological issues has relied on synthetic chemical pesticides. While their use has indeed increased productivity, it is also evident their detrimental and cumulative effects on the environment, and the current negative perception of the population toward these chemicals. In response, governments are prompting the search for alternatives to synthetic pesticides, through different policies, such as the strategy From Farm to Fork in the European Union, which aims to reduce the use of chemical pesticides by 50% by 2030, among other measures. At this point, seed extracts with biocidal activity are emerging as a viable option for the control and management of various pathogenic agents, such as harmful bacteria, fungal and oomycete pathogens, and plant-parasitic nematodes. Nevertheless, it is worth mentioning that most of the studies have been only conducted under highly controlled conditions. Thus, this line of research should be still more deeply developed, including proofs under field conditions, in order to become the extensive and widespread use of these bio-products a reality. In this review, we compile the main studies focused on the use of these compounds for phytosanitary purposes, describing and analysing the key metabolites, their composition, extraction processes and the mechanisms involved in their antagonistic effects. Additionally, we analyse the primary factors contributing to the limited adoption of these extracts in the field, such as the scarcity of studies under real conditions or the possible impact on non-target organisms, and discuss future prospects for their development.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1878818124003165/pdfft?md5=83da21975b98647c970eb61ffd38be7e&pid=1-s2.0-S1878818124003165-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141964095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mixed culture fermentation using Lactobacillus delbrueckii and novel Lactiplantibacillus plantarum strains isolated from traditional chhurpi from Sikkim Himalayan region for potential probiotic and improved functional properties","authors":"","doi":"10.1016/j.bcab.2024.103338","DOIUrl":"10.1016/j.bcab.2024.103338","url":null,"abstract":"<div><p>The aim of the study was to isolate potential probiotic lactic acid bacteria (LAB) strains from the traditional fermented milk products, <em>Chhurpi</em> of the Sikkim Himalayan region and to prepare curd using mixed culture fermentation. A preliminary probiotic assessment of 110 LAB isolates including antimicrobial activity, bile and acid tolerance, surface hydrophobicity, auto-aggregation and co-aggregation, and adhesion to Caco-2 cells, was performed. <em>Lactiplantibacillus plantarum</em> strains N4, CHDE2, CHDE4, and SS22 demonstrated better viability in bile (0.3%) and acid (pH 2.5), adherence with hydrophobicity (13.93–92.85%), auto-aggregation (72.44–89.74%), adhesion to Caco-2 cells (45–73.61%) and antimicrobial activity against food borne pathogens. These strains were used for mixed culture fermentation with a previously screened curd forming strain <em>Lactobacillus delbrueckii</em> WS4. Curd produced using mixed culture fermentation of <em>Lb. delbrueckii</em> WS4 and the selected <em>Lp. plantarum</em> strains had a relatively higher acid production (pH 3.97–4.01), protein hydrolysis, and antioxidant activity. Further, mixed culture fermentation also resulted in the increase of HOCl scavenging activity (12.22–32.96%), myeloperoxidase (MPO) inhibition activity (22.38–42.94%) and angiotensin converting enzyme (ACE) inhibition (15.61–33.03%) as compared to that of WS4 fermentation (P &lt; 0.05). This is the first report on identification of potential probiotic strains from the traditional fermented milk products of Sikkim and application in mixed culture fermentation with enhanced multifunctional potential.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141984998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lytic polysaccharide monooxygenases producing microbes: A key indicator for biomass-degrading enzymes","authors":"","doi":"10.1016/j.bcab.2024.103337","DOIUrl":"10.1016/j.bcab.2024.103337","url":null,"abstract":"<div><p>The transition of agricultural wastes into value added, sustainable end-products are governed by microbial decomposition through production of different lignolytic enzymes for renew energy and low carbon (C) economy for the future. The discovery of lytic polysaccharide monooxygenases <strong>(</strong>LPMOs), uplift the activity of glycoside hydrolases with other glycolytic enzymes for rapid degradation of agricultural waste. A better understanding of LPMO's active sites, co-substrates, electron donors, inhibiting factors and other structural aspects will assure their position in industry to convert non-edible biomass into fuel, energy or other useful compounds. This review insight about the classification, biological aspect, structural information, catalytic mechanism and applications of LPMOs. Nonetheless, production of LPMOs is discussed along with the amplification of genes, as its production will reduce the enzyme load required for the conversion of ligno-cellulolytic agricultural biomass.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141991371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of co-application of iron and silicon on the root plaque formation in rice plants (Oryza sativa L.) grown in arsenic infested soil of gangetic Bengal, India","authors":"","doi":"10.1016/j.bcab.2024.103335","DOIUrl":"10.1016/j.bcab.2024.103335","url":null,"abstract":"<div><p>The present work is a biomarker-based analysis to understand reduction in arsenic toxicity in rice plants due to co-application of iron and silicon. Both iron and silicon have been co-applied in the soil of the potted rice plants with a specific dose of arsenic. The results showed, increase in root arsenic content with only Fe application (E1) as 115% and decrease in shoot arsenic content as 35.4% against control, with Fe + Si application (E2) the root arsenic increased 44% compared to E1 and shoot arsenic content decreased almost 50%, 73% and 25% increase in root Fe content in E1 and E2 is due to formation of Fe-plaque on the root surface which is further justified by the SEM image. The increased arsenic content in the plant root is due to deposition of arsenic in the root Fe-plaque. Plant health parameters revealed that 18% increase in Carbohydrate in E1 compared to control and 9% increase in E2 compared to E1, chlorophyll and protein content followed a similar trend. Malonaldehyde (MDA) content revealed a decrease of 20% in E1 and E2 plants. Super Oxide-dismutase (SOD), an antioxidant enzyme, the unique biomarker to understand the plant stress, showed a decreasing trend of 11% in E1 compared to control and more than 50% in E2 compared to E1, the expression study of the SOD-gene also showed a positive correlation with the biochemical data.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141954059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insilico sequence-structure based analysis of bacterial chromate reductase to unravel enzymatic specificity towards chromium pollution","authors":"","doi":"10.1016/j.bcab.2024.103339","DOIUrl":"10.1016/j.bcab.2024.103339","url":null,"abstract":"<div><p>Extensive use of chromium in industrial sectors leads to hazardous consequences of chromium pollution. The hexavalent chromium is much more damaging to biological systems than the trivalent and other forms. The best strategy for bioconversion of such toxic hexavalent chromium to less toxic trivalent chromium is the enzymatic breakdown using chromate reductase produced by several bacteria. The employment of in silico analysis aids in the quick detection of a protein's function and stability. Therefore, an insilico sequence and structure analysis of chromate reductase of the different bacterial groups has been performed. Amino acid variation reveals more charged polar residues in the extremophilic group than the mesophiles and is more hydrophilic. According to secondary structure data, beta-bulge formation was only observed in <em>Thermus scotoductus</em>. A higher abundance of network salt bridges and isolated aromatic-sulphur interactions were observed in extremophiles, indicating higher stability. Rhombus network salt bridge formation is a novel finding in thermophilic bacteria. Better isolated cation-pi interactions were observed in both thermophiles and halophiles. Molecular dynamics simulation studies through RMSD observed similar trajectories between thermophilic and psychrophilic proteins indicating a shared structural pattern. RMSF studies indicate the lowest fluctuations by thermophiles compared to other groups. Rg values indicate that chromate reductase of <em>Halomonas subglaciescola</em> had the lowest plot indicating its most compactness. The chromate reductase from psychrophilic <em>Psychrobacillus psychrotolerans</em> displayed the lowest SASA, indicating the most stability. Hydrogen bond number was also higher in extremophiles. This is the first report on such comparative analysis of chromate reductase from extremophiles indicating that they are more preferred candidates in providing a more stable enzyme for better chromium remediation.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141963127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable cultivation of Porphyridium cruentum via agro-industrial by-products: A study on biomass and lipid enhancement","authors":"","doi":"10.1016/j.bcab.2024.103341","DOIUrl":"10.1016/j.bcab.2024.103341","url":null,"abstract":"<div><p>This study explored the cultivation of <em>Porphyridium cruentum</em> using beet molasses and corn steep liquor (CSL) as alternative agri-waste substrates. The objective was to enhance the biomass and lipid production of <em>P. cruentum</em>, known for its potential as an industrial EPA producer. Using a Box-Behnken Design (BBD) as part of the response surface methodology (RSM), we investigated the effects of beet molasses, CSL, and F/2 nutrients on the microalga's cultivation. The results demonstrated a significant influence of these factors on the final cell count and lipid content over an 8-day cultivation period. Specifically, optimal growth conditions were identified at approximately 1.78 g/L of molasses and 1.89 g/L of CSL, yielding a cell count of 12.1 × 10⁶ cells/mL and a lipid content of 24.48%. Validation experiments reaffirmed these findings, with observed results closely aligning with predicted values. Optimized media achieved also a significant higher content of carotenoids and phycobiliproteins compared to the standard media. This research highlights the potential of using agro-industrial by-products for large-scale cultivation of <em>P. cruentum</em>, offering a sustainable approach for enhancing lipid and pigment production.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1878818124003256/pdfft?md5=f008442489d72caa9e18f7caad936c78&pid=1-s2.0-S1878818124003256-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141993409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resilient mechanism of strigolactone (GR24) in regulating morphological and biochemical status of maize under salt stress","authors":"","doi":"10.1016/j.bcab.2024.103340","DOIUrl":"10.1016/j.bcab.2024.103340","url":null,"abstract":"<div><p>Strigolactones (SLs), relatively recent plant growth regulators, bolster plant resilience in challenging environmental conditions and augment signaling networks. Salinity, a well-established abiotic stressor, threatens global crop cultivation and productivity. We hypothesized that the synthetic SL analogue GR24 could alleviate salinity stress in maize by reducing oxidative damage and enhancing various growth and physiological attributes. To explore this hypothesis, we subjected two maize hybrids, PB-ProA-2018 and PB-ProA-2019, to seed priming with three GR24 concentrations (non-soaked, water-soaked, 0.001, 0.01, 0.1 mg/L) for 16 h. Seeds were then germinated in sand culture under two salt stress levels [ 0 (Control) and 100 mM NaCl] and nourished with Hoagland's nutrient medium. Salt stress significantly stunted maize growth attributes. The pre-soaking seed treatment with 0.01 mg L⁻¹ GR24 demonstrated a significant enhancement in plant growth metrics, yielding a 31.3% increase in root length and a 62% augmentation in total leaf area per plant, in comparison to non-soaked yet stressed plants. Salt stress led to increased activities of catalase and peroxidase, along with higher levels of leaf ascorbic acid, malondialdehyde, H₂O₂, glycine betaine, and free proline, compared to control seedlings. Pre-soaking with 0.01 mg L⁻¹ GR24 further upregulated superoxide dismutase (SOD) by 29.6%, peroxidase (POD) by 68.3%, catalase (CAT) by 25%, ascorbic acid by 23.1%, free proline by 42.3%, and total phenolics content by 13%, compared to stressed counterparts. Notably, the 0.01 mg L⁻¹ GR24 concentration exhibited superior efficacy in mitigating salt stress effects, with PB-ProA-2019 outperforming PB-ProA-2018 among the maize hybrids. These findings advance our understanding of SLs' potential in alleviating salinity stress in maize, offering valuable insights for crop resilience enhancement.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141963128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth characteristics and molecular identification of indigenous Limnospira strains from Ethiopian soda lakes as a protein source","authors":"","doi":"10.1016/j.bcab.2024.103336","DOIUrl":"10.1016/j.bcab.2024.103336","url":null,"abstract":"<div><p>Malnutrition poses a significant global challenge in regions such as Ethiopia, particularly in children with limited dietary diversity. <em>Arthrospira</em>, a protein-rich cyanobacterium known for its rapid growth even under extreme conditions, is a promising solution for biomass production. This study addresses knowledge gaps in the morphological characterization and taxonomic classification of <em>Arthrospira</em> by focusing on precise species identification and strategic strain selection for optimal biomass production. Cyanobacterial strains were cultivated in a modified Spirulina Ogawa Terui medium at 35 °C under the photosynthetically active radiation of 160 μmol photons m⁻² s⁻¹ with a 12/12 h light/dark period in laboratory conditions. One hundred <em>Arthrospira-</em>like strains were isolated and characterized based on their morphological attributes according to accepted criteria, from Ethiopian soda lakes. Systematic evaluation of the growth rate comparison test identified four exceptionally fast-growing strains, with LC-30 exhibiting the highest dry weight (3.27 ± 0.51 g L^˗1) and specific growth rate (1.63 ± 0.05 d^˗1) among them. DNA analysis confirmed that all four examined strains belong to the <em>Limnospira</em> genus, specifically identified as <em>Limnospira fusiformis</em>. LC-30 and LA-08 demonstrated protein and phycocyanin levels (582.80 ± 0.07 and 557.90 ± 0.09 mg g^˗1, respectively) comparable to those reported for <em>Arthrospira platensis</em>, and surpassing conventional protein sources, thus suggesting their potential for nutritional supplementation. The study highlights the importance of bioprospecting indigenous strains from soda lakes, revealing promising candidates for large-scale biomass production.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141963126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced production of high-value compounds from Chlorella sorokiniana by two-stage cultivation under red light and salinity stress","authors":"","doi":"10.1016/j.bcab.2024.103315","DOIUrl":"10.1016/j.bcab.2024.103315","url":null,"abstract":"<div><p>Microalgae produce valuable compounds in harsh environments, making them a promising resource for several industries. The traditional cultivation methods fail to achieve commercial viability, leading to decreased biomass production and high-value compound synthesis even under stressful conditions. To address these challenges, we conducted a two-stage cultivation to examine the impact of various LED lights and high salinity stress on the biomass and high-value compounds of the microalgae <em>Chlorella sorokiniana</em> UUIND6. When compared to the control, red light and 2-fold salinity performed best. The experimental results of the two-stage culture strategy demonstrated a substantial increase (p &lt; 0.05) in photosynthetic pigments under red light conditions but a loss under 2-fold salinity during the second stage. Under red-light illumination, pigment concentration increased by 3.17% (chlorophyll ‘a'), 10.58% (chlorophyll ‘b'), and 62.21% for carotenoids, but was reduced by 2-fold salinity. Under 2-fold salt stress, the maximum lipid content was increased by 22.54%, followed by red LED light (15.35%). Microalgae cell size was also measured and found to be larger under red light stress (5.3 μm) and smaller under salt stress (3.3 μm). The presence of polyunsaturated fatty acids (PUFAs), monounsaturated fatty acids (MUFAs), and other molecules was verified by GC-MS and FTIR tests. Additionally, UHPLC demonstrated the considerable presence of both amino acids. The primary objective of the research was to enhance the yield of valuable compounds by improving knowledge of cultivation techniques and appropriate abiotic stressors. One such technique is the two-stage cultivation method, which enables the production of valuable compounds under diverse growth circumstances or stressors.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141736649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}