Biocatalysis and agricultural biotechnology最新文献

{"title":"Enhancing stability and transglycosylation efficiency of alternansucrase from Leuconostoc citreum ABK-1 by immobilization","authors":"Kusuma Thongplaew ,&nbsp;Karan Wangpaiboon ,&nbsp;Pawinee Panpetch ,&nbsp;Rath Pichyangkura ,&nbsp;Thanapon Charoenwongpaiboon ,&nbsp;Manchumas Prousoontorn","doi":"10.1016/j.bcab.2025.103671","DOIUrl":"10.1016/j.bcab.2025.103671","url":null,"abstract":"<div><div>Alternansucrase (ALT) catalyzes the transglycosylation of sucrose, synthesizing α-1,6 and α-1,3-linked glucan, and also utilizes maltose as an acceptor to produce glucooligosaccharides. In this study, alternansucrase from <em>Leuconostoc citreum</em> ABK-1 was immobilized on glutaraldehyde-activated chitosan beads. Immobilization parameters, namely glutaraldehyde concentration and enzyme loading, were optimized to achieve 59.7 % activity yield with an immobilized activity of 10.3 U/g bead. The immobilized ALT exhibited a broader working pH than that of free enzyme, with an optimal pH shift from 4 to 5–6. The thermostability of immobilized ALT was significantly improved, exhibiting up to a 5.4-fold increase in half-life at 37 °C. DLS analysis revealed that the immobilized ALT produced larger alternan particles, with an average diameter of 117.3 nm compared to 79.75 nm for the free enzyme, suggesting that transglycosylation efficiency was enhanced. Also, the immobilized ALT could synthesize a higher amount of isomaltose and isomaltotriose. Moreover, we found that the immobilized enzyme retained over 50 % of its initial activity after 14 reuse cycles, suggesting its potential for industrial applications of immobilized ALT due to enhanced stability and reusability.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103671"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144570948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential gene expression analysis reveals transcription factors that control flower initiation and development in Cannabis sativa","authors":"Ayelign M. Adal ,&nbsp;Reza Sajaditabar,&nbsp;Tien Nguyen,&nbsp;Soheil S. Mahmoud","doi":"10.1016/j.bcab.2025.103691","DOIUrl":"10.1016/j.bcab.2025.103691","url":null,"abstract":"<div><div>RNA-Seq and differential gene expression analysis were employed to identify transcription factors (TF) that are specifically expressed in developing buds, and potentially control flower timing and organ development in <em>C. sativa</em>. We found 199 TFs that were upregulated and 140 TFs that were downregulated in floral buds compared to young leaves. Of these, 16 exhibited strong homology to MADS Box type TFs that control flower timing and pattern development in other plants. For proof of concept, we assessed the <em>in planta</em> roles of two SEPALLATA-type TFs through the stable overexpression of these genes in <em>Arabidopsis thaliana</em> plants. Transgenic plants overexpressing <em>csSEP3</em> grew at a slower rate compared to wild-type plants during the early growth stages, but they caught up to wild-type plants after 20 days of growth. On the other hand, transgenic plants overexpressing <em>csSEP1</em> exhibited stunted growth, and produced floral buds about 10 days earlier than the wild-type controls. At the time of bolting, these plants had 8.5 ± 0.2 rosette leaves, while transgenic plants overexpressing <em>csSEP3</em> and the wild-type plants had 31.8 ± 3.9 and 38.4 ± 2.9 leaves, respectively. Flower pattern development was not affected in either of the transgenic plants. Not surprisingly, production of the floral scent constituents (monoterpenes) was not affected in either transgenic plant. The differentially expressed genes (in particular TFs) highlighted in this study represent a valuable resource for further investigating the molecular basis of flower development in <em>C. sativa</em>.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103691"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144623331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking Nature's Vault: Endophytes as plant-sourced biological treasures","authors":"Arnab Chakraborty,&nbsp;Rajshree Amrit,&nbsp;Prerona Dutta,&nbsp;W. Jabez Osborne","doi":"10.1016/j.bcab.2025.103685","DOIUrl":"10.1016/j.bcab.2025.103685","url":null,"abstract":"<div><div>Endophytes, a consortium of symbiotic microorganisms residing in plant tissues, have emerged as a potential aspect in shaping plant health, ecological dynamics, and various biotechnological sectors, including agriculture and pharmaceuticals. They exhibit a remarkable taxonomic diversity, extending to a broad spectrum of bacterial and fungal communities, with their distribution linked to several abiotic and biotic factors. Understanding mechanisms underlying endophyte colonization, establishment, and signalling pathways involved in endophyte-host interactions has become crucial in bioprospecting novel bioactive metabolites and biocontrol of pests and pathogens. This review aims to underscore the pivotal roles of medicinal plant endophytes in modulating plant growth dynamics and the biosynthesis of various classes of bioactive compounds with notable applications in the fields of pharmaceuticals, bioremediation and agriculture. The novelty of this review lies in its attempt to collate ethnobotanical knowledge of medicinal plants with the technicalities of modern OMICs technologies, such as metabolomics and metagenomics, which in recent years have been pivotal for screening and characterization of bioactive compounds and in understanding the mechanistic perspectives of intermolecular networking. These OMICs-based technologies also provide insights into the genes and metabolites synthesized by endophytes, offering an in-depth understanding of the various metabolic profiles and genomic characteristics of endophytic population dynamics. Coupling endophyte bioactive compound bioprospecting from various underexplored ecosystems with modern synthetic biology techniques could also potentially open up novel avenues leading to the development of “Magic bullets” to address the rising threat of antimicrobial resistance (AMR). The challenges, limitations, and future perspectives in endophytic research, therefore, require detailed evaluation to encompass the positive aspects of the lead molecules synthesized by the endophytes, which could benefit the pharmaceutical and agricultural sectors.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103685"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144579411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of niobium-doped ZnO nanoparticles using Eucalyptus globulus leaf extract for enhanced photocatalytic dye degradation and antibacterial applications","authors":"Tasmia Amanuallah ,&nbsp;Iqra Muneer ,&nbsp;Dilawar Ali ,&nbsp;Asad Abbas ,&nbsp;Imama Arif ,&nbsp;Farhat Yasmeen","doi":"10.1016/j.bcab.2025.103687","DOIUrl":"10.1016/j.bcab.2025.103687","url":null,"abstract":"<div><div>This study presents a novel green hydrothermal synthesis of niobium (Nb)-doped zinc oxide (ZnO) nanoparticles using <em>Eucalyptus globulus</em> leaf extract as a natural reducing and stabilizing agent. Nb doping was performed at 2 %, 5 %, and 8 % concentrations to optimize photocatalytic and antibacterial performance. Structural and morphological analyses via FTIR, XRD, DLS, EDX, SEM, and UV–Vis spectroscopy confirmed successful synthesis. XRD revealed a hexagonal wurtzite structure with crystallite sizes between 24 and 27 nm, and DLS showed hydrodynamic diameters ranging from 93 to 141 nm. UV–Vis analysis demonstrated a significant band gap reduction from 3.21 eV (pure ZnO) to 2.15 eV for 8 % Nb-doped ZnO, enhancing visible light absorption. The 8 % Nb/ZnO sample achieved 98 % degradation of methylene blue within 120 min under sunlight—outperforming lower doping levels and many reported green-synthesized ZnO systems. Additionally, it exhibited strong antibacterial activity against both Gram-positive and Gram-negative strains. These results highlight the effectiveness of optimized Nb doping and plant-mediated synthesis in producing multifunctional nanomaterials for sustainable water treatment and microbial control.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103687"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144579646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Germination and growth responses of milk thistle (Silybum marianum) to seed priming with selenium and nano-selenium under salinity conditions","authors":"Elham Havangi ,&nbsp;Moslem Rostampour ,&nbsp;Hossein Hammami","doi":"10.1016/j.bcab.2025.103679","DOIUrl":"10.1016/j.bcab.2025.103679","url":null,"abstract":"<div><div>Salt stress is a significant factor that impacts on seed germination, establishment, and growth of plants. This study investigated the impact of selenium and nano-selenium seed priming on the germination and growth characteristics of milk thistle under salinity conditions. Consequently, two experiments were arranged separately as a completely randomized factorial design with three replications. In the 1st and 2nd experiment, seeds of milk thistle were subjected to seven priming levels (control (no priming), 5, 10, and 15 mg/L selenium, and 5, 10, and 15 mg/L nano-selenium) and four salinity stress levels (0, 20, 40, and 60 mM NaCl) in laboratory and greenhouse setting, respectively. Salinity and priming treatments showed significantly affected all germination characteristics. Significant effects were detected in GR, MGT, RL, SFW, SDW, and SVI concerning the interaction between salinity and priming. The highest GR, SL, SFW, SDW, and SVI, along with the lowest MGT were recorded at 15 mg/L of nano-selenium across all salinity levels in comparison to non-priming treatments. Salinity and priming treatments significantly affected all plant attributes in pot studies, with the exception of Chl b. The interaction between salinity and priming significantly affected PH, RL, RDW, SDW, RWC, and antioxidant activity characteristics. In both non-salinity and salinity conditions, the 15 mg/L nano-selenium exhibited the most pronounced growth characteristics across all features. Overall, 15 mg/L nano-selenium treatments markedly improved germination and growth characteristics of milk thistle under salinity stress conditions.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103679"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Larvicidal activity, oxidative stress, and enzymatic alterations induced by essential oil from Piper cyrtopodon (Miq.) C.DC (Piperaceae) on Aedes aegypti (Culicidae)","authors":"Maria L.L. da Costa ,&nbsp;André C. de Oliveira ,&nbsp;Vinícius B. Ribeiro ,&nbsp;Suelen C. Lima ,&nbsp;Felipe M.A. da Silva ,&nbsp;Sergio M. Nunomura ,&nbsp;Rita de C. S. Nunomura ,&nbsp;Rosemary A. Roque ,&nbsp;Tatiane P. de Souza","doi":"10.1016/j.bcab.2025.103661","DOIUrl":"10.1016/j.bcab.2025.103661","url":null,"abstract":"<div><div>Dengue, Zika, and chikungunya are mosquito-borne diseases transmitted by <em>Aedes aegypti</em>, with over 13 million dengue cases, 431.223 chikungunya cases, and 44.242 Zika cases reported in the Americas in 2024. The resistance of <em>Ae. aegypti</em> to synthetic insecticides has driven the search for botanical alternatives, such as essential oils (EOs) from <em>Piper</em> species. This study evaluated the EO from <em>Piper cyrtopodon</em> and its main compounds as larvicides, investigating their mechanism of action. The EO, extracted by hydrodistillation (yield: 1.4 ± 0.2 %), contained germacrene D (57.46 %), cadina-1(10),4-diene (10.57 %), and <em>δ</em>-elemene (8.0 %). It showed significant larvicidal activity against <em>Ae. aegypti</em>, with LC₅₀ values ranging from 20.78 to 146.61 μg/mL over 24 and 48 h. Cadina-1(10),4-diene was the most active compound (<em>p</em> &lt; 0.0001). Biochemical analyses revealed oxidative stress in larvae, with increased reactive oxygen species production (11 ± 2 to 26 ± 2 μmol H₂O₂/g), lipid peroxidation (0.54 ± 0.06 to 2.8 ± 0.22 ηmol MDA/g), and protein oxidation (1.62 ± 0.04 to 4.54 ± 0.51 nmol/mg protein) compared to controls (<em>p</em> &lt; 0.0001). Antioxidant enzymes superoxide dismutase (10.33 ± 3 to 28.33 ± 6 mU/mg protein), catalase (1.04 ± 0.09 to 7.08 ± 2.42 mmol H₂O₂/min/mg protein), and glutathione peroxidase (5.40 ± 1.37 to 14.67 ± 1.52 mmol NADPH/min/mL) were altered, suggesting redox imbalance as a toxicity mechanism. These findings highlight the potential of the EO from <em>P. cyrtopodon</em> as a natural alternative for <em>Ae. aegypti</em> control.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103661"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144534281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Zeatin–auxin synergy and growing media optimization enhanced micropropagation, acclimatization, and antioxidant properties of ‘Bentong’ ginger (Zingiber officinale Roscoe)” [Biocatal. Agric. Biotechnol. 67 (2025) 103653]","authors":"Nisar Ahmad Zahid ,&nbsp;Hawa Z.E. Jaafar ,&nbsp;Mansor Hakiman","doi":"10.1016/j.bcab.2025.103680","DOIUrl":"10.1016/j.bcab.2025.103680","url":null,"abstract":"","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103680"},"PeriodicalIF":3.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144572009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zeatin–auxin synergy and growing media optimization enhanced micropropagation, acclimatization, and antioxidant properties of ‘Bentong’ ginger (Zingiber officinale Roscoe)","authors":"Nisar Ahmad Zahid ,&nbsp;Hawa Z.E. Jaafar ,&nbsp;Mansor Hakiman","doi":"10.1016/j.bcab.2025.103653","DOIUrl":"10.1016/j.bcab.2025.103653","url":null,"abstract":"<div><div>‘Bentong’ ginger (<em>Zingiber officinale</em> Roscoe) is a medicinally and economically important ginger cultivar in Malaysia. However, its conventional propagation via rhizomes is limited by low multiplication rates and a high risk of disease transmission. This study aimed to develop an efficient micropropagation protocol by optimizing the synergistic application of zeatin and auxins. Murashige and Skoog (MS) medium supplemented with 10 μM zeatin and 2.5 μM 1-naphthaleneacetic acid (NAA) significantly enhanced shoot multiplication (6.72 shoots per explant) and overall <em>in vitro</em> shoot development. Furthermore, the effects of different growing media on <em>ex vitro</em> acclimatization were evaluated. Plantlets grown in a 1:1:1 mixture of soil, coco peat, and vermiculite exhibited the highest survival rate (94.79 %) and superior vegetative and physiological performance, followed closely by a mixture of soil, peat moss, and vermiculite (93.75 % survival). Phytochemical analyses revealed no significant differences in total polyphenols content or antioxidant activities, assessed via 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging and iron-chelating assays, between rhizomes of micropropagated and conventionally propagated plants. These findings highlight the potential of zeatin–auxin synergy and optimized acclimatization media to efficiently produce disease-free, true-to-type ‘Bentong’ ginger planting materials.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103653"},"PeriodicalIF":3.4,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144501420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural characterization, functional analysis and computational annotation of a metagenome-derived Glucoamylase enzyme: Effect of temperature, pH, metal Ions, and surfactants on enzyme activity","authors":"Atif Khurshid Wani ,&nbsp;Alaa S. Alhegaili ,&nbsp;Faisal Imam ,&nbsp;Chirag Chopra ,&nbsp;Juliana Heloisa Pinê Américo-Pinheiro ,&nbsp;Farida Rahayu ,&nbsp;Aniswatul Khamidah ,&nbsp;Gatut Wahyu Anggoro Susanto ,&nbsp;Runik Dyah Purwaningrahayu ,&nbsp;Mudasir A. Dar ,&nbsp;Reena Singh","doi":"10.1016/j.bcab.2025.103662","DOIUrl":"10.1016/j.bcab.2025.103662","url":null,"abstract":"<div><div>Glucoamylase (GluAmy) holds significant industrial relevance, particularly in starch processing industries, owing to its ability to efficiently hydrolyze complex carbohydrates into glucose under diverse environmental conditions. In this study, a thermostable and alkalophilic GluAmy gene, 510 bp in length, was amplified from a hot spring metagenome. The gene was initially cloned into the pJET 1.2 vector and transformed into <em>Escherichia coli</em> DH5α, followed by heterologous expression using the pET28a vector in <em>E. coli</em> BL21 (DE3) cells. Purification via Ni-His affinity chromatography yielded GluAmy (19.2 kDa), which was biochemically characterized for activity and stability across a wide pH range (3.0–12.0) and temperatures (10°C–110 °C). The enzyme's activity was influenced by metal ions (Mn²⁺, Mg²⁺, Ca²⁺, Zn²⁺, Fe²⁺, Na⁺, Co²⁺, Cu²⁺, Ni²⁺) at 5–10 mM concentrations, as well as surfactants (Tween-20, Tween-80, Triton X-100, SDS) at 5–10 %. GluAmy demonstrated optimal activity at 80 °C and pH 9.0. Co²⁺ and Ca²⁺ enhanced activity by 115.2 % and 105.6 %, respectively, whereas Tween-20 reduced activity to 56.3 %. The purified enzyme exhibited the highest specific activity against starch (12.94 U/mg), followed by dextrin (11.67 U/mg) at 1 % substrate concentration. Computational analysis revealed a protein structure predominantly composed of random coils (53.25 %), contributing to its thermal stability. These findings underscore the potential of GluAmy as a robust biocatalyst for industrial applications, particularly in processes requiring high temperature and alkaline conditions.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103662"},"PeriodicalIF":3.4,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis of silver nanoparticles using Bougainvillea flower extract: Antibacterial, antibiofilm and cytotoxicity studies","authors":"Mohammad Azam Ansari ,&nbsp;Mohammad N. Alomary","doi":"10.1016/j.bcab.2025.103663","DOIUrl":"10.1016/j.bcab.2025.103663","url":null,"abstract":"<div><div>This study investigated a sustainable approach for synthesizing silver nanoparticles (AgNPs) utilizing an extract from the flower of <em>Bougainvillea glabra</em> plant. The successful synthesis of AgNPs was confirmed by various biophysical techniques: UV–Vis spectroscopy revealed a prominent peak (432 nm), whilst FT-IR and XRD investigations confirmed their chemical composition and crystalline structure. TEM investigations indicated that the AgNPs measured around an average of 25.05 ± 0.87 nm and exhibited a spherical morphology. MIC values against gram-positive and gram-negative ranged from 32 to 128 μg/mL. The XTT assay shows that AgNPs at 500 μg/mL proficiently inhibits bacterial biofilm by 96 %. Light and electron microscopy investigations have indicated that AgNPs dramatically restrict their colonization and adhesion. SEM and TEM show that the cell surface exhibited irregularities, roughness, distortion, and an impaired state, characterized by a defective cellular membrane and its wall, signifying an impairment of membrane integrity that subsequently leads to cell death. Furthermore, the AgNPs exhibited a regulated toxic effect on breast cancer cells, inducing apoptosis while maintaining biocompatibility and sparing healthy cells. The promising results suggest that this eco-friendly way of making AgNPs could lead to new methods for delivering drugs to fight cancer and treat infections associated to biofilms.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"67 ","pages":"Article 103663"},"PeriodicalIF":3.4,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144492143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}