Biocatalysis and agricultural biotechnology最新文献

{"title":"Plant growth-promoting Bacillus cereus MCC3402 facilitates rice seedling growth under arsenic-spiked soil","authors":"Pallab Kumar Ghosh ,&nbsp;Krishnendu Pramanik ,&nbsp;Kalyan Mahapatra ,&nbsp;Sayanta Mondal ,&nbsp;Sudip Kumar Ghosh ,&nbsp;Antara Ghosh ,&nbsp;Tushar Kanti Maiti","doi":"10.1016/j.bcab.2024.103405","DOIUrl":"10.1016/j.bcab.2024.103405","url":null,"abstract":"<div><div>Microorganisms can solve the global problem of arsenic (As) contamination in soil and water, by eliminating the hazardous metalloid from contaminated habitats. A native As-resistant PMM6 strain was isolated from the tainted agricultural field in Durgapur, India. Following phenotypic investigations, fatty acid methyl ester analysis, and 16S rDNA sequence-based homology, it was identified as <em>Bacillus cereus</em>. Various analytical techniques such as atomic absorption spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, X-ray fluorescence, scanning electron microscopy, transmission electron microscopy and energy dispersive X-ray spectroscopy studies revealed a positive correlation between the strain's resistance properties against arsenate (75 mM) and arsenite (25 mM) and the biosorption and bioaccumulation of arsenic. The strain exhibited several important plant growth-promoting traits, including indole-3-acetic acid production (110.0±0.33μg/mL), 1-aminocyclopropane-1-carboxylate deaminase activity (2334.2±1.90 nm α-ketobutyrate/mg protein/h), phosphate solubilization, and siderophore production (68.0±2%). These traits facilitated the growth augmentation of rice seedlings under As stress, both in terms of physiological and biochemical parameters. Compared to uninoculated soil, the use of strain PMM6 helped to reduce As mobilization and oxidative stress in rice seedlings growing in As-spiked soil. Therefore, strain PMM6 holds the potential as bioremediator to restore As-contaminated agricultural lands while also promoting the growth of rice seedlings. It could thus be utilized in the bioremediation of As-contaminated agricultural lands in the near future.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Valorization of agricultural by-product for cleaner and sustainable production of microbial pigments and cellulolytic enzymes in a zero-waste approach","authors":"Yasmi Louhasakul ,&nbsp;Sutima Preeprem ,&nbsp;Warunee Hajimasalaeh ,&nbsp;Nisaporn Muhamad ,&nbsp;Benjamas Cheirsilp","doi":"10.1016/j.bcab.2024.103397","DOIUrl":"10.1016/j.bcab.2024.103397","url":null,"abstract":"<div><div>This work presents a zero-waste concept for the valorization of banana peel into <em>Monascus</em> value-added metabolites, such as pigments and cellulolytic enzymes, and for evaluation of the feasibility of treated peel as a promising source for animal feedstuff. <em>M. purpureus</em> YRU01 exhibited satisfactory pigment and cellulolytic enzyme production in solid-state fermentation. Particularly, repeated solid-state fermentation of non-sterile peel with 90% substrate replacement was effective in offering high recovery of pigments, xylanase, and cellulase (7.19-, 31.19-, and 92.62-fold higher than those of solid-state fermentation, respectively). The LC/MS profile of fermented peel provided important evidence of fungal metabolites (&gt;100 metabolites) for understanding and evaluating the fermentation. Moreover, citrinin-related mycotoxin fragments were not found. The treated peel had high carbohydrate, neutral detergent fiber, and acid detergent fiber contents, but low protein content, indicating its potential to be used as a roughage in animal feed.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micro-nano bubbles in action: AC/TiO2 hybrid photocatalysts for efficient organic pollutant degradation and antibacterial activity","authors":"Chakkawan Boonwan ,&nbsp;Thammasak Rojviroon ,&nbsp;Orawan Rojviroon ,&nbsp;Ranjith Rajendran ,&nbsp;Shanmugam Paramasivam ,&nbsp;Ragavendran Chinnasamy ,&nbsp;Sabah Ansar ,&nbsp;Supakorn Boonyuen ,&nbsp;Roongrojana Songprakorp","doi":"10.1016/j.bcab.2024.103400","DOIUrl":"10.1016/j.bcab.2024.103400","url":null,"abstract":"<div><div>Developing highly active and sustainable photocatalysts is crucial for environmental remediation. This work focuses on the enhanced photocatalytic degradation efficiency of synthetic dyes using TiO₂-coated AC hybrid photocatalysts under MNB aeration. The TiO₂-coated AC hybrid photocatalyst (HP) was synthesized via a sol-gel method and characterized using XRD, FTIR, SEM, EDS, HR-TEM, and DRS. The study investigated the photocatalytic degradation of three dyes indigo carmine (IC), reactive black 5 (RB5), and methylene blue (MB) in wastewater, with initial dye concentrations ranging from 10 to 100 μM. Under UVA irradiation, the hybrid photocatalyst with MNB aeration (HP + UVA + MNB) achieved the highest degradation efficiencies of 69.09%, 60.06%, and 55.19% for IC, MB, and RB5, respectively. Further analysis showed that the chromophores and complex structures of these dyes were broken into intermediate products. The Langmuir-Hinshelwood kinetic model was used to describe the reaction mechanisms. The HP + UVA + MNB system demonstrated superior photocatalytic degradation activity, making it suitable for operational and environmental applications in dye wastewater treatment. Additionally, the antibacterial properties of AC and HP were tested at 100 μg/mL, showing effectiveness against both Gram-positive <em>Staphylococcus aureus</em> and Gram-negative <em>Escherichia coli.</em></div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production and optimization of biofuels from locally isolated algal biomass: Strategies for circular economy integration","authors":"Muhammad Uzair Javed ,&nbsp;Iqra Nazeer ,&nbsp;Hamid Mukhtar ,&nbsp;Umer Rashid ,&nbsp;Maria J. Sabater ,&nbsp;Fahad A. Alharthi","doi":"10.1016/j.bcab.2024.103383","DOIUrl":"10.1016/j.bcab.2024.103383","url":null,"abstract":"<div><div>In the present study, we investigated the potential of locally isolated algal strains as alternative energy sources for sustainable biofuel production. The focus of this study was to identify algal strains that are capable of accumulating oils rich in essential fatty acids. Algal samples were collected from different areas and 14 isolates were obtained. Among the various pretreatment methods tested, hydrothermal pretreatment using sulfuric acid at 95 °C yielded the best results, with sample IIB-14 containing more than 2% reducing sugars. These sugars were then used for fermentation with the <em>S. cerevisiae</em> strain, resulting in an ethanol concentration of 3.52% ± 0.2%. This holistic approach contributes to the development of low-cost and environmentally friendly alternatives to traditional energy sources. While algal biofuels offer a promising substitute for fossil fuels, further advancements are needed before they can be widely adopted in the fuel market. Among these, isolates IIB-8 and IIB-9 showed the highest oil yields of 22.84% and 24.69% (w/w), respectively. The specific environmental settings for optimal growth of these strains were determined, and the physicochemical parameters of the oils, including iodine value, viscosity, density, acid value, saponification value, unsaponifiable mass, and peroxide value, were analyzed. The transesterification of oils into fatty acid methyl esters (FAMEs) revealed the presence of significant amounts of fatty acids, including EPA, DHA, and linoleic acid. Moreover, the study also explored the potential of algal biomass for bioethanol production, addressing the sustainability concerns of renewable energy supplies. Hydrothermal pretreatment using sulfuric acid at 95 °C yielded the highest concentration of reducing sugars (&gt;2%) in IIB-14. Sugar extracted from algal biomass was used for fermentation. The <em>Saccharomyces cerevisiae</em> strain used for the fermentation process yielded an ethanol concentration of 3.52% ± 0.2%. This holistic approach contributes to the development of low-cost and environment-friendly alternatives to renewable energy sources. Algal biofuels may offer a practical substitute for fossil fuels, but there is still a long way to go before they can enter the fuel market and are widely used.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One-pot synthesis of stable vitamin C analogue using trans-glycosylating enzyme from Aspergillus carbonarius BTCF 5","authors":"Reshma M. Mathew ,&nbsp;Meena Sankar ,&nbsp;Athiraraj Sreeja-Raju ,&nbsp;Prajeesh Kooloth-Valappil ,&nbsp;Anoop Puthiyamadam ,&nbsp;Dileep Raveendran-Nair ,&nbsp;Rajeev K. Sukumaran","doi":"10.1016/j.bcab.2024.103401","DOIUrl":"10.1016/j.bcab.2024.103401","url":null,"abstract":"<div><div>Biocatalysis using transglycosidases for one-pot glycosylation of small molecules is a greener alternative to chemical glycosylation. The transglycosylation potential of the crude culture filtrate of <em>Aspergillus carbonarius</em> BTCF 5 was evaluated based on the biotransformation of the target molecule, ascorbic acid (Vitamin-C), to its more stable glycosylated analogue -2-O-α-D-glucopyranosyl ascorbic acid (AA2G), using maltose as the glycosyl donor. Media engineering increased the transglycosylation yield by 64%, whereas the use of concentrated culture filtrate increased the transglycosylation yield by 70%. Protein purification studies identified the candidate transglycosylating enzyme to be a GH 31 family CAZyme (α-transglucosidase). A minimally purified enzyme could enhance the transglycosylation yield by 77% with a total AA2G yield of 91.5 mM (10.32 g/L) and improved the overall conversion rate of ascorbic acid and maltose in the reaction by 16% and 6.3% respectively from the base level. A two-step column chromatography helped in product purification with 46% recovery. The structural characterization of purified AA2G provided a detailed insight into the regioselectivity of the enzyme to glycosylate C2 of ascorbic acid and its ability to form AA2G as the predominant isomer, which is also the most preferred one for end applications.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasible biosynthesis of biologically active metabolites in in vitro culture of Macrotyloma uniflorum","authors":"Sannia Batool ,&nbsp;Mehnaz Khanum ,&nbsp;Hasnat Tariq ,&nbsp;Gouhar Zaman ,&nbsp;Muhammad Uzair Javed ,&nbsp;Bilal Haider Abbasi ,&nbsp;Christophe Hano ,&nbsp;Nathalie Giglioli-Guivarc'h","doi":"10.1016/j.bcab.2024.103374","DOIUrl":"10.1016/j.bcab.2024.103374","url":null,"abstract":"<div><div><em>Macrotyloma uniflorum,</em> commonly called “Horse gram” is an underutilized pulse crop recognized for its great nutritional significance and a broad range of biological properties. There have been no <em>in vitro</em> studies for the biosynthesis of enhanced bioactive metabolites in the callus culture of <em>M. uniflorum</em>. In the study reported here, we have designed a feasible protocol for high-frequency callus induction and maintenance utilizing a leaf as an explant grown on MS media enriched with various concentrations of different plant growth regulators (PGRs) including α-naphthalene acetic acid (NAA), 6-benzylaminopurine (BAP) and thidiazuron (TDZ) either alone or in combination. Among all the tested PGRs, NAA alone resulted in high-frequency callus induction (97%), and maximum biomass accumulation (Fresh weight (FW): 200 g/L: Dry weight (DW): 20.4 g/L). Moreover, hormonally optimized callus cultures exhibit maximum production of phenolic compounds (166.6 mg/L) and flavonoid compounds (351.6 mg/L). The antioxidant potential of calli extracts was also determined by utilizing various antioxidant activities. Maximum antioxidant activities ((2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)) ABTS = 497.9; TEAC μM; (Ferric reducing antioxidant power) FRAP = 823 TEAC μM; (Cellular Antioxidant Activity) CAA: 58.2%) were recorded in leaf-derived calli supplemented with different PGRs treatments. High-performance liquid chromatography (HPLC) analysis further revealed maximum biosynthesis of caffeic acid (1.63 mg/g DW), gallic acid (8.92 mg/g DW), kaempferol (0.71 mg/g DW), myricetin (0.39 mg/g DW), apigenin (0.64 mg/g DW) at 10 mg/L BAP and isorhamnetin (0.68 mg/g DW) at 1 mg/L TDZ + 10 mg/L NAA. The objective of this study was to explore <em>in vitro</em> biosynthesis of enhanced bioactive metabolites in the callus culture of <em>M. uniflorum</em>, leveraging a feasible protocol for high-frequency callus induction and maintenance. The results showcase the remarkable efficacy of NAA in callus induction and biomass accumulation, highlighting the hormonally optimized callus cultures as a potent source for enhanced biosynthesis of bioactive metabolites, paving the way for further applications in pharmaceutical and commercial industries.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marine natural products for biofouling elimination in marine environments","authors":"Saba Ghattavi ,&nbsp;Ahmad Homaei ,&nbsp;Pedro Fernandes","doi":"10.1016/j.bcab.2024.103385","DOIUrl":"10.1016/j.bcab.2024.103385","url":null,"abstract":"<div><div>Biofouling is a broad word encompassing marine organisms such as animals, microorganisms, and plants that grow when immersed in both normal and abnormal amounts of saltwater. There are several biofouling species in the water that easily adhere to sea equipment and cause its rapid destruction. The use of antifouling coating or paint can effectively stop marine biofouling. The use of chemical antifouling coatings containing metals such as copper, tin, etc. has been restricted and banned by the International Marine Environment Organization. So, an increasing number of researchers are focusing on eco-friendly antifouling coatings. Within this scope, the use of marine natural compounds with antifouling action has emerged as a significant research area. Marine bioactive compounds are plentiful, inexpensive, efficient, environmentally friendly, long-lasting, and biodegradable, making them ideal for antifouling applications. In this review, we reviewed and discussed the characteristics of biofouling growth and development mechanisms, the sources of marine bioactive compounds with antifouling activity, their chemical structure, and the organisms that they inhibit. This paper fosters marine organisms and their bioactive compounds as potential prospects for future commercial applications, in particular the antifouling activity of marine ecosystems on marine equipment.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marine protease-producing bacteria as potential probionts for advancing mariculture","authors":"Rasiya Sultana ,&nbsp;Imelda Joseph","doi":"10.1016/j.bcab.2024.103392","DOIUrl":"10.1016/j.bcab.2024.103392","url":null,"abstract":"<div><div>The research delves into the isolation and characterization of protease-producing bacteria from seawater, sediment, and the gastrointestinal tracts (GITs) of Snubnose pompano (<em>Trachinotus blochii</em>) for potential use as probiotics in marine finfish culture. Five bacterial strains screened from the marine environmental samples-<em>Chryseobacterium indologenes Chryseobacterium culicis, Bacillus aerius</em>, <em>Bacillus altitudinis</em>, and <em>Bacillus pumilus</em> were isolated and characterized both phenotypically and genotypically. Protease activity was assessed across acidic to alkaline pH levels (5–10) and temperatures (20 °C–120 °C) to identify the optimum conditions. <em>B. pumilus</em>, isolated from the GIT of <em>T. blochii</em>, exhibited the highest protease activity (0.088 Unit/ml) at pH 6 and 40 °C, making it a particularly potent strain. <em>B. altitudinis</em> and <em>B. aerius</em> also demonstrated significant protease activity at pH 7, with values of 0.086 Unit/ml and 0.081 Unit/ml, respectively. The <em>in vitro</em> study suggests that all three <em>Bacillus</em> strains, have the potential to serve as probiotics in marine finfish mariculture, emphasizing <em>B. pumilus</em> due to its superior protease activity under defined conditions. This research contributes to the development of tailored probiotics for improving protein digestibility in mariculture systems, advancing sustainable practices in marine finfish production.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing bioencapsulation of yeast cells by Aspergillus tubingensis TSIP9 and applications in bioethanol production through repeated-batch fermentation","authors":"Zeeba Saeed ,&nbsp;Benjamas Cheirsilp ,&nbsp;Wageeporn Maneechote ,&nbsp;Prawit Kongjan ,&nbsp;Rattana Jariyaboon","doi":"10.1016/j.bcab.2024.103377","DOIUrl":"10.1016/j.bcab.2024.103377","url":null,"abstract":"<div><div>This study aimed to immobilize yeast cells using filamentous fungi owing to a number of advantages including less chemical usage, spontaneous encapsulation, strong adhesion and biocompatibility. Filamentous fungus, <em>Aspergillus tubingensis</em> TSIP9 could form sphere-shape and packed pellets using either fungal fresh spores or those stored in liquid medium for 6 weeks at 4 °C. The fungus was used to immobilize yeast <em>Saccharomyces cerevisiae</em> FAI via adsorption and co-cultivation methods. Scanning electron microscopy images revealed that the bioencapsulated yeast cells via co-cultivation seemed to be more tightly adhered on fungal mycelia and surrounded by extracellular polymeric substances. The yeast biocapsules also exhibited higher stability and maintained their structural integrity during repeated-batch fermentation while the immobilized yeast cells by adsorption gradually degraded during their repeated uses. The bioethanol production from glucose by yeast biocapsules were in the range of 95–98 g/L with the bioethanol yield of 0.49–0.54 g-ethanol/g-consumed glucose. The yeast biocapsules could produce bioethanol well when using enzymatic hydrolysate of lignocellulosic palm waste, as alternative cheap carbon source, with the comparable bioethanol yield of 0.49 ± 0.17 g-ethanol/g-consumed glucose. The spontaneous and inter-species bioencapsulation show the perspectives as active biocatalysts with high cell retention for repeated uses.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of antifungal properties in medicinal plant extracts for sustainable agriculture","authors":"Nusrat Ahmad,&nbsp;Mansoor Ahmad Malik,&nbsp;Mohd Yaqub Bhat,&nbsp;Abdul Hamid Wani","doi":"10.1016/j.bcab.2024.103398","DOIUrl":"10.1016/j.bcab.2024.103398","url":null,"abstract":"<div><div>The escalating environmental repercussions of pesticide use in pest and disease management underscore the urgency for alternative strategies. One promising avenue involves harnessing natural antifungal properties present in plant extracts. This study aimed to assess the antifungal efficacy of aqueous and ethanolic extracts from five medicinal plants, viz., <em>C. sativa</em> L., <em>S. moorcraftiana</em> L., <em>S. nigrum</em> L., <em>F. vesca</em> L., and <em>R. pseudoacacia,</em> which were screened for their antifungal activity against various soil-borne fungi, viz., <em>A. niger</em>, <em>A. flavus</em>, <em>A</em>. <em>terreus</em>, <em>R. stolonifer, M. mucedo, F. oxysporum, A. alternata, P. notatum, C. cladosporioides,</em> and <em>C. lunata</em>. Phytochemical analysis exhibited the presence of phenols, alkaloids, tannins, flavonoids, quinines, and terpenoids. Results revealed potent antifungal activity across all tested fungi, significantly inhibiting mycelial growth compared to control. <em>S. nigrum</em> and <em>C. sativa</em> extracts exhibited the highest efficacy against multiple fungal pathogens. The inhibition in mycelial growth in ethanolic and aqueous plant extracts of <em>C. sativa</em> against <em>A. niger</em> varies between 3.00 mm to 6.00 mm and 4.00 mm–6.33 mm, against <em>A. flavus</em> 3.33 mm–11.66 mm and 4 mm–12.66 mm, <em>A. terreus</em> (3.00 mm–6.00 mm and 3.66 mm–7.00 mm), <em>R. stolonifer</em> (3.00 mm–5.33 and 3.66 mm–6.33 mm), <em>M. mucedo</em> (8.33 mm–17.66 mm and 9.66 mm–18.66 mm), <em>F. oxysporum</em> (6.66 mm–12.33 mm and 7.33 mm–13.33 mm), <em>A. alternata</em> (4.66 mm–11.33 mm and 5.33 mm–14.00 mm), <em>P. notatum</em> (9.33 mm–23.00 mm and 9.66 mm–24.33 mm), <em>C. cladosporioides</em> (4.33 mm–8.66 mm and 5.33 mm–9.66 mm), and <em>C. lunata</em> (14.00 mm–20.33 mm and 17.00 mm–21.33 mm) in different concentrations of plant extracts respectively. Likewise, the inhibition in mycelial growth in ethanolic and aqueous plant extracts of <em>S. nigrum</em> against <em>A. niger</em> at different concentrations varies between 8.00 mm to 17.66 mm and 9.00 mm–18.66 mm, <em>A. flavus</em> (8.33 mm–12.66 mm and 9 mm–14 mm), <em>A. terreus</em> (2.33 mm–5.66mm and 3 mm–7 mm), <em>R. stolonifer</em> (13.33 mm–20.33 mm and 17.66 mm–24.66 mm), <em>M. mucedo</em> (10.66 mm–12.00 mm and 11.66 mm–13.00 mm), <em>F. oxysporum</em> (15.66 mm–22.33 and 18.00 mm–24.00 mm), <em>A. alternata</em> (10.66 mm–13.00 mm and 11.66 mm–14.00 mm), <em>P. notatum</em> (5.33 mm–13.33 mm and 6.33 mm–18.66 mm, <em>C. cladosporioides</em> (3.33 mm–7.66 mm and 4.00 mm–8.00 mm), and <em>C. lunata</em> (3.66 mm–6.33 mm and 4.66 mm–6.66 mm), respectively. These findings suggest that these plant extracts have the potential to be natural fungicides, providing promising alternatives for disease management in agriculture.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}