Biocatalysis and agricultural biotechnology最新文献

{"title":"Antimicrobial efficacy of CS-SeNPs against pathogens in aquaculture: A potential trade-off with fish gut health?","authors":"Sowmiya Prasad ,&nbsp;Tharmathass Stalin Dhas ,&nbsp;Sherlin John ,&nbsp;Prathas Selvaraj ,&nbsp;J. Francis Borgio ,&nbsp;Sayed AbdulAzeez ,&nbsp;Inbakandan Dhinakarasamy ,&nbsp;Anandakumar Natarajan ,&nbsp;Ramesh Kumar Varadharajan ,&nbsp;Karthick Velu ,&nbsp;C.M. Vineeth Kumar","doi":"10.1016/j.bcab.2025.103760","DOIUrl":"10.1016/j.bcab.2025.103760","url":null,"abstract":"<div><div>Aquatic pathogens pose a major challenge to the aquaculture industry, leading to significant economic losses. Selenium-based nanoparticles (SeNPs) are emerging as effective antimicrobials, but their impact on beneficial fish microbiota remains underexplored. This study evaluates the antimicrobial efficacy and cytotoxicity of chitosan–selenium nanoparticles (CS-SeNPs) against aquatic pathogens and fish gut bacteria. CS-SeNPs were synthesized by heat-assisted synthesis and characterized using various spectroscopic techniques (UV–Vis, FTIR, DLS, XRD, and Raman Spectroscopy), Antimicrobial and cytotoxic effects were tested against <em>Vibrio cholerae</em> and <em>Shewanella algae</em> (aquaculture wastewater isolates) and <em>Brachybacterium</em> sp. and <em>Mammalicoccus sciuri</em> (fish gut isolates). Cytotoxicity, lipid peroxidation and ROS generation assays were performed across a concentration gradient (up to 1000 μg/mL) to evaluate dose-dependent responses. CS-SeNPs showed Amax at 250 nm, indicating the formation of CS-SeNPs. XRD spectrum revealed amorphous nature of CS-SeNPs and a Raman analysis peak at 251 cm⁻¹, indicated presence of localized monoclinic Se and Se₈. The average particle size was 164 nm. CS-SeNPs showed maximum cytotoxicity of 73.65 % (<em>V. cholerae</em>), 99.06 % (<em>S. algae</em>), 75.88 % (<em>Brachybacterium</em> sp.), and 89.0 % (<em>M. sciuri</em>). Corresponding lipid peroxidation values were 73.7 %, 99.06 %, 69.68 %, and 74.90 %, respectively, suggesting ROS-mediated cell damage. ROS production was estimated by DCFH-DA assay and confirmed elevated ROS levels. CS-SeNPs exhibited potent antimicrobial activity via oxidative stress mechanisms but also significantly affected beneficial gut bacteria. These findings highlight the need for cautious application of CS-SeNPs in aquaculture to prevent unintended microbiome disruption and ensure sustainable disease management.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103760"},"PeriodicalIF":3.8,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145020390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of laccase from Salipiger sp. CCB-MM3 with decolorization activity against azo, anthraquinone and triarylmethane dyes","authors":"Kharisma Panji Ramadhan ,&nbsp;Dede Heri Yuli Yanto ,&nbsp;Aik-Hong Teh","doi":"10.1016/j.bcab.2025.103757","DOIUrl":"10.1016/j.bcab.2025.103757","url":null,"abstract":"<div><div>Laccases are multicopper oxidases with potential applications in the decolorization of synthetic dyes such as azo, anthraquinone and triarylmethane dyes. The laccase from the marine bacterium <em>Salipiger</em> sp. CCB-MM3, Lac_MM3, is a monomer with a predicted Greek-key β barrel typical of laccases. Characterization of Lac_MM3 revealed that Cu²⁺ acted as an activator with an apparent binding constant of 174 μM. The presence of a peak at 600 nm indicated that Cu²⁺-loaded Lac_MM3 was a typical blue laccase. It functioned optimally at 40 °C within a narrow pH range of 3.0–4.0, with <em>k</em>_cat/<em>K</em>_m of 113.3 s⁻¹mM⁻¹ against the substrate ABTS. Decolorization assays towards 14 synthetic dyes showed that Lac_MM3 efficiently decolorized the azo dyes Direct Blue 71, Congo Red, and Acid Blue 113, as well as the anthraquinone dye Acid Blue 129. The addition of ABTS as a mediator had mixed effects on the decolorization of these dyes. In addition, immobilization of Lac_MM3 in an alginate matrix resulted in three-fold enhancement of enzyme reusability.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103757"},"PeriodicalIF":3.8,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145057394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring different environmental impacts: How abiotic factors shape flavonoid synthesis in plants","authors":"Hurmat ,&nbsp;Richa Shri ,&nbsp;Parul Sood ,&nbsp;Gagandeep Kaur","doi":"10.1016/j.bcab.2025.103726","DOIUrl":"10.1016/j.bcab.2025.103726","url":null,"abstract":"<div><div>Flavonoids are important class of secondary plant metabolites naturally distributed in flowers, fruits, seeds, and various plant parts. For plants, few colorful flavonoids (mainly anthocyanins) are of immense importance as a colorful pigment that acts as pollinator attractants while others are colorless and serve different biological functions. Moreover, it also plays important role in plant growth and development by acts as UV light protectants; as growth regulators; for cell signaling; to protect against environmental stresses and as defense molecules. Thus, flavonoids act as stress relievers and coping compounds for plants. The key stimulus for the biosynthesis of flavonoids is often abiotic stress. A plethora of evidence shows that flavonoid biosynthesis is influenced by abiotic stresses such as flooding, drought, soil salinity, low/high light intensity, metal stress, high temperature, nutrient scarcity etc. and makes a significant contribution to the response mechanisms of different medicinal herbal plants. Thus, appropriate abiotic factors can play a critical role in enhancing biosynthesis of medicinally and commercially important flavonoids. These factors can be incorporated during cultivation of flavonoid containing medicinal herbal plants for enhancing flavonoid biosynthesis as its accumulation behaviour varies depending on the developmental stage, species and even cultivars of the same species. This review not only highlights that molecular structure, classification and biosynthesis of flavonoid but also summarizes the impact on quality, quantity, and variety of flavonoids in plants as an adaptive response to cope up with unfavorable ecological conditions is also included.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103726"},"PeriodicalIF":3.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144920363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous application of hydrogen peroxide, hydrogen sulfide, and potassium phosphite enhances rosmarinic acid accumulation and modulates key biosynthetic gene expression in Melissa officinalis L","authors":"Nahid Abdi ,&nbsp;Nima Ahmadi ,&nbsp;Ali Pakdin-Parizi ,&nbsp;Marie-Laure Fauconnier","doi":"10.1016/j.bcab.2025.103754","DOIUrl":"10.1016/j.bcab.2025.103754","url":null,"abstract":"<div><div><em>Melissa officinalis</em> L. (lemon balm) is a well-known medicinal plant traditionally valued for its pharmacological properties. Its aerial parts are rich in bioactive compounds such as rosmarinic acid (RA), which play key roles in the plant defense system and contribute to its antispasmodic, sedative, and memory-enhancing activities. However, the natural concentrations of these secondary metabolites are typically low in medicinal plants. This study investigated the influence of exogenous elicitors, hydrogen peroxide (HP, 5 and 10 mM), hydrogen sulfide (HS, 0.25 and 0.5 mM), and potassium phosphite (PP, 0.5 and 1 g/L) on the accumulation of photosynthetic pigments, antioxidant enzyme activity, phenolic content, RA production, and the expression of RA biosynthesis-related genes. All elicitors significantly increased chlorophyll <em>a, b</em>, and carotenoid levels. Treatment with HP and HS notably elevated total phenolic and flavonoid contents. The activity of CAT and APX enzymes varied depending on the elicitor type and concentration applied. Elicitation enhanced RA accumulation by enhancing PAL activity and modulating the expression of key biosynthetic genes (<em>PAL</em>, <em>4CL</em>, <em>TAT</em>, and <em>HPPR</em>). HS treatment upregulated genes in both phenylalanine and tyrosine pathways, whereas HP was more effective in stimulating the tyrosine pathway. PP elicitation led to differential gene expression depending on its concentration. These results demonstrate that HP, HS, and PP can modulate key metabolic and signaling pathways, thereby boosting RA production and potentially enhancing the medicinal value of <em>M. officinalis</em> in a safe and environmentally friendly manner.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103754"},"PeriodicalIF":3.8,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144922792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The assessment of antimicrobial and antibiofilm activities, molecular docking studies, and the photodegradation of methylene blue by green-fabricated ZnO nanoparticles using the plant extract of Helichrysum noeanum boiss","authors":"Suzan Sahin Dogan ,&nbsp;Duygu Aydin ,&nbsp;Bugrahan Emsen ,&nbsp;Burak Surmen","doi":"10.1016/j.bcab.2025.103755","DOIUrl":"10.1016/j.bcab.2025.103755","url":null,"abstract":"<div><div>In recent years, natural reducing agents have been studied worldwide to reduce harmful effects of toxic chemicals utilized in nanoparticle production. Zinc oxide nanoparticles (ZnO NPs) have been prepared for their photocatalytic potential in treating industrial dyes, for example methylene blue (MB). Therefore, the present study reports synthesizing and assessing antimicrobial effectiveness of ZnO NPs biosynthesized with an endemic plant <em>Helichrysum noeanum</em> Boiss (<em>H. noeanum</em>). Optical, morphological, and surface area features of synthesized ZnO NPs were evaluated utilizing UV–visible spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and Energy dispersive X-ray analysis (EDX) and X-ray diffraction (XRD), and determined the chemical composition and purity ZnO NPs as well as DLS and zeta potential analysis. The interactions between phytoconstituents in aqueous extract of <em>H. noeanum</em> and ZnO NPs were identified from FT-IR spectra. Dye degradation efficiency of ZnO NPs (5 mg/mL) was found as 81 % against MB under sunlight. Additionally, minimum inhibitory concentration (MIC) values for, <em>S. aureus</em> ATCC 29213, <em>E. coli</em> ATCC 43895, <em>B. cereus</em> NRRL B-3711<em>, P. aeruginosa</em> ATCC 27853, and <em>S. typhi</em> were 75, 50, 50, 100, 50 μg/mL, respectively. Furthermore, ZnO NPs at a concentration of 100 μg/mL reduced 94 % of the biofilm formed by P. aeruginosa. Also, molecular docking was performed to evaluate the binding interactions between ZnO NPs and protein targets involved in microbial pathogenesis and biofilm activity, including PaAP, elastase, and pqsA. Therefore, this study offers a straightforward and effective method for synthesizing ZnO NPs mediated with <em>H. noeanum</em> with potent antimicrobial activity and for wastewater treatment by degrading various toxic dyes.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103755"},"PeriodicalIF":3.8,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144922795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ag/TiO2 photocatalysts: A promising tool in combating bacterial resistance","authors":"Caroline Zarzzeka ,&nbsp;Jonas Goldoni ,&nbsp;Filomena Marafon ,&nbsp;Gilnei Bruno da Silva ,&nbsp;Daiane Manica ,&nbsp;Paulo Rodrigo Stival Bittencourt ,&nbsp;Margarete Dulce Bagatini ,&nbsp;Leda Maria Saragiotto Colpini","doi":"10.1016/j.bcab.2025.103751","DOIUrl":"10.1016/j.bcab.2025.103751","url":null,"abstract":"<div><div>The photoactivation of materials is emerging as a promising strategy, as it allows pathogens to be inactivated without the selection of resistant strains. The identification and development of new drugs or photoactive agents represents an active field of research, with the aim of offering more effective therapeutic options for the control of bacterial infections. This study synthesized silver-based catalysts (Ag) (2 % and 10 % w/w%) supported on titanium dioxide (TiO₂) by the sol-gel method (2AgT/SG and 10AgT/SG). Infrared spectrometric characterization of AgT/SG catalysts revealed characteristic titanium bands and the asymmetric vibration of Ti-Ag-O. X-ray diffraction identified that these catalysts have well-defined anatase peaks representing organized crystalline phases. The differential scanning calorimetry data shows smaller endothermic peaks for the AgT/SG catalysts, indicating a low melting point. Thermogravimetric analysis showed minimal loss of mass for the catalysts. The 10AgT/SG catalyst exhibited antibacterial activity against <em>Escherichia coli</em> under clear light (9 W) and in the dark, due to the Ag concentration. Under black light, all catalysts activated, eliminating both <em>E. coli</em> (ATCC 25922) and <em>Staphylococcus aureus</em> (ATCC 25923) in just 10 min. This rapid bactericidal action was attributed to the massive production of reactive oxygen species (ROS), which was 1500 % higher than at the beginning of the experiment for the tested strains. These results demonstrate the potential of AgT/SG catalysts, presenting a material that overcomes the limitations of pure TiO₂ and, acts quickly, paving the way for practical applications in disinfection and infection-fighting devices.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103751"},"PeriodicalIF":3.8,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144922794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional composition, phytochemical profile, HPLC analysis, and antioxidant activity of leaf and flower extracts of Rumex nervosus from Yemen","authors":"Muneer Mohammed Saleh Alsayadi ,&nbsp;Mufeda Ahmed Hazea Gazaem ,&nbsp;Sharifah Aminah Syed Mohamad ,&nbsp;Nor Hadiani Ismail ,&nbsp;Mohammed A.A. Alshoaiby ,&nbsp;Hasan M. Agha ,&nbsp;Abdulmutalib Alabeed Alkamil ,&nbsp;Mustapha Salihu ,&nbsp;Fatimah Salim","doi":"10.1016/j.bcab.2025.103749","DOIUrl":"10.1016/j.bcab.2025.103749","url":null,"abstract":"<div><div><em>Rumex nervosus</em> is a widely used medicinal plant in Yemen, traditionally valued for its therapeutic properties. This study aims to address this gap by evaluating and comparing the phytochemical composition and antioxidant activities of leaf and flower extracts of <em>R. nervosus</em>. Ethanol and water extracts of the leaf and flower were screened for key phytochemicals, including tannins, saponins, flavonoids, terpenes/steroids, and alkaloids. The total phenols content (TPC), Total flavonoids (TFC), and total tannins (TTC) were quantified, and the antioxidant activity was assessed using 2,2-diphenyl-1-picrylhydrazyl, hydroxyl radical scavenging, 2,2′-azinobis(3-ethylbenzthiazolin-6-sulfonic acid radical scavenging, ferric reducing power, potassium ferricyanide reducing power, and total antioxidant capacity. The results revealed that Leaves showed higher TPC/TFC, while flowers had superior TTC and nutritional content P ≤ 0.05. The total phenolic, flavonoid, and tannin content in <em>R. nervosus</em> ranged from 121.16 ± 27.80 to 299.42 ± 6.45 mg GAE/g, 169.72 ± 13.03 to 241.59 ± 1.87 mg QE/g DM, and 254.33 ± 13.59 to 261.72 ± 2.51 mg CE/g, respectively. It showed significantly higher levels of TPC, TFC in the ethanolic extracts than in the water extracts (P ≤ 0.05). Both leaf and flower extracts exhibited strong antioxidant activity across all methods, comparable to ascorbic acid. The antioxidant activities were found to correlate with the phytochemical content, suggesting that both leaf and flower extracts of <em>R. nervosus</em> could serve as promising sources of nutraceuticals, natural antioxidants, and therapeutic alternatives.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103749"},"PeriodicalIF":3.8,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative toxicity and physiological effects of pyriproxyfen and sweet wormwood essential oil on Papilio demoleus L. (Lepidoptera: Papilionidae)","authors":"Amir Hossein Yasaman,&nbsp;Zahra Afrazeh,&nbsp;Azim Nemati,&nbsp;Roya Azizi,&nbsp;Jalal Jalali Sendi","doi":"10.1016/j.bcab.2025.103753","DOIUrl":"10.1016/j.bcab.2025.103753","url":null,"abstract":"<div><div>Citrus crops face infestation by over 250 insect pests across all growth stages, with the lemon butterfly (<em>Papilio demoleus</em> L.) being a major foliar pest. This study investigated the toxicity and physiological effects of <em>Artemisia annua</em> essential oil (EO) and Pyriproxyfen (Py) a synthetic insect growth regulator (IGRs) as positive control on 5th instar larvae of <em>P. demoleus</em>. Oral toxicity bioassays revealed that Py exhibited significantly higher acute toxicity (LC₅₀ = 0.027 W/V%) compared to <em>A. annua</em> EO (LC₅₀ = 4.09 W/V%). Sublethal concentrations (LC₃₀) of both compounds adversely affected larval nutritional physiology, reducing key indices such as approximate digestibility (AD) and efficiency of conversion of ingested food (ECI). Py caused more pronounced suppression of these parameters, suggesting stronger metabolic disruption. Biochemical analyses demonstrated that both treatments modulated detoxification enzymes (e.g., esterases, glutathione S-transferases (GST), and cytochrome P450) and antioxidant defenses (Catalase), with Py inducing greater oxidative stress. Total Hemocyte Count (THC) and Differential Hemocyte Count (DHC) were significantly reduced, indicating compromised immunity. While Py showed superior lethality, <em>A. annua</em> EO exhibited multifaceted bioactivity, including antifeedant and growth-regulatory effects. These findings highlight the potential of <em>A. annua</em> EO as a sustainable alternative for integrated pest management (IPM) of <em>P. demoleus</em>, balancing efficacy with environmental safety.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103753"},"PeriodicalIF":3.8,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144917931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fed-batch strategies for growing β-carotene enriched yeast Rhodotorula paludigena using untreated molasses","authors":"Chatchol Kongsinkaew ,&nbsp;Yusuf Chisti ,&nbsp;Mariena Ketudat-Cairns ,&nbsp;Supenya Chittapun ,&nbsp;Soisuda Pornpukdeewattana ,&nbsp;Theppanya Charoenrat","doi":"10.1016/j.bcab.2025.103750","DOIUrl":"10.1016/j.bcab.2025.103750","url":null,"abstract":"<div><div>The β-carotene-rich yeast <em>Rhodotorula paludigena</em> CM33 was grown in a 22-L bioreactor using sugarcane molasses in combination with several distinct fed-batch strategies: DO (dissolved oxygen)-stat; stepwise feeding; and hybrid feeding (an initial exponential feeding phase followed by a DO-stat operation). The impacts of feeding strategies on biomass production, carotenoid production and nutrient conversion efficiencies were evaluated. The stepwise feeding proved most effective, achieving: a final dry biomass concentration of 73.1 ± 2.7 g L⁻¹; a β-carotene titer of 181.9 ± 1.0 mg L⁻¹; a biomass volumetric productivity of 1.33 ± 0.05 g L⁻¹ h⁻¹; and β-carotene productivity of 3.31 ± 0.02 mg L⁻¹ h⁻¹. However, with this feeding strategy the sugar and nitrogen conversion efficiencies were the lowest: the β-carotene yield on total sugar was 0.92 ± 0.00 mg g⁻¹ and its yield on nitrogen was 5.47 ± 0.06 mg g⁻¹. In contrast, the DO-stat operation was the most resource efficient, providing a biomass yield on sugar of 0.47 ± 0.02 g g⁻¹, a biomass yield on nitrogen of 2.64 ± 0.05 g g⁻¹, a β-carotene yield on sugar of 1.08 ± 0.04 mg g⁻¹, and β-carotene yield on nitrogen of 6.08 ± 0.08 mg g⁻¹. The hybrid strategy resulted in intermediate values of the various performance parameters. Raw sugarcane molasses proved to be excellent for high-cell-density culture of <em>R. paludigena</em>. A compromise between productivity and resource use efficiency may be required for commercial production of β-carotene especially if the substrate is expensive compared to the product.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103750"},"PeriodicalIF":3.8,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144917935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Silver nanoparticles for control of foliar pathogens in passion fruit (Passiflora edulis) crops” [Biocatal. Agric. Biotechnol. 68 (2025) 103717]","authors":"Natália Deniz Brito ,&nbsp;Rafael José Vilela de Oliveira ,&nbsp;Felipe Leon Nascimento de Sousa ,&nbsp;Ana Júlia Silva Rocha ,&nbsp;Maria Eduarda Almeida Matos ,&nbsp;Laura Lima Turani ,&nbsp;Raphael Ferreira Queiroz ,&nbsp;Bianca Galúcio Pereira Araújo ,&nbsp;Giovanna Machado ,&nbsp;Quelmo Silva de Novaes","doi":"10.1016/j.bcab.2025.103752","DOIUrl":"10.1016/j.bcab.2025.103752","url":null,"abstract":"","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103752"},"PeriodicalIF":3.8,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}