Biocatalysis and agricultural biotechnology最新文献

{"title":"Marine natural products for biofouling elimination in marine environments","authors":"","doi":"10.1016/j.bcab.2024.103385","DOIUrl":"10.1016/j.bcab.2024.103385","url":null,"abstract":"<div><div>Biofouling is a broad word encompassing marine organisms such as animals, microorganisms, and plants that grow when immersed in both normal and abnormal amounts of saltwater. There are several biofouling species in the water that easily adhere to sea equipment and cause its rapid destruction. The use of antifouling coating or paint can effectively stop marine biofouling. The use of chemical antifouling coatings containing metals such as copper, tin, etc. has been restricted and banned by the International Marine Environment Organization. So, an increasing number of researchers are focusing on eco-friendly antifouling coatings. Within this scope, the use of marine natural compounds with antifouling action has emerged as a significant research area. Marine bioactive compounds are plentiful, inexpensive, efficient, environmentally friendly, long-lasting, and biodegradable, making them ideal for antifouling applications. In this review, we reviewed and discussed the characteristics of biofouling growth and development mechanisms, the sources of marine bioactive compounds with antifouling activity, their chemical structure, and the organisms that they inhibit. This paper fosters marine organisms and their bioactive compounds as potential prospects for future commercial applications, in particular the antifouling activity of marine ecosystems on marine equipment.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spirulina biomass loaded with iron nanoparticles: A novel biofertilizer for the growth and enrichment of iron content in rice plants","authors":"","doi":"10.1016/j.bcab.2024.103387","DOIUrl":"10.1016/j.bcab.2024.103387","url":null,"abstract":"<div><div>The current investigation was focused to develop a nano-iron-based phycofertilizer to enhance crop quality in an environment-friendly manner. Biosynthesis of nano-iron occurred when <em>Spirulina</em> biomass was exposed to a 0.05M FeCl₃ solution for 48h at pH 5.5 in the dark, resulting in the synthesis of spindle-shaped nano-iron both inside and outside the cells. The particles' sizes were 24.77 ± 5.264 nm as observed in transmission electron microscopy. Further characterizations were carried out using energy dispersive X-ray spectroscopy (EDX), and dynamic light scattering (DLS). The performance of various fertilizers, including those derived from phycosynthesized nano iron (INP), dried <em>Spirulina</em> biomass (SB), and nano-iron loaded <em>Spirulina</em> biomass i.e., nano-phycho-fertilizer (NPF) was compared against conventional NPK fertilizer. The results from field applications indicated significant improvements in rice, with NPF outshining the rest. Notably, NPF demonstrated a remarkable 37.6% increase in shoot length, a 47% boost in crop productivity, and a 15% rise in grain weight of rice compared to NPK. Both unpolished and polished grains of NPF-treated plants contained higher iron contents (44% and 28%) than the NPK-treated plants. Furthermore, the cost-effectiveness analysis underscored the superiority of NPF over conventional fertilizer, representing it as a highly effective and eco-friendly nano-iron-based phycofertilizer.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable extraction of bioactive compounds from aromatic plants and agro-food wastes for food preservation: A review","authors":"","doi":"10.1016/j.bcab.2024.103399","DOIUrl":"10.1016/j.bcab.2024.103399","url":null,"abstract":"<div><div>The demand of natural bioactive compounds in diverse sectors like food, pharmaceuticals, and cosmetics are increasing day-after-day, and thus significant development in novel extraction of such functional and active compounds from natural resources have been achieved. This review paper discusses about various techniques of sustainable and innovative extraction of bioactive compounds from agro-food wastes, and their use as food preservatives. Several novel techniques, such as microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), supercritical fluid extraction (SCFE), pulsed electric field (PEF) extraction, etc., as well as many combined/integrated (two or more) techniques including ultrasound-microwave-assisted extraction (UMAE), and ultrasound-assisted pressurized liquid extraction (UAPLE) are elaborated. Moreover, applications of these natural bioactive compounds in food preservation, maintaining quality, and shelf-life extension are also summarized. This comprehensive study provides valuable insights into the practical implications of novel extraction techniques and their role in meeting the increasing demand for bioactive compounds for various food applications. Bioactive phenolic compounds recovered from agro-food wastes have been successfully incorporated into foods to achieve higher shelf-life. Additionally, environment-friendly extraction methods, like ultrasound and microwave assisted integrated techniques are advantageous in extraction of antioxidant and preservatives phytocompounds.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing phosphorus availability and growth of green gram (Vigna radiata) in acidic red and laterite soil through liquid formulations of native phosphate solubilizing bacteria","authors":"","doi":"10.1016/j.bcab.2024.103413","DOIUrl":"10.1016/j.bcab.2024.103413","url":null,"abstract":"<div><div>Phosphorus (P) availability in acid soils is often limited due to its fixation with various soil components. This leads to low use efficiency of P fertilizers and raises environmental concerns when they are used in excess. Phosphate-solubilizing bacteria (PSB) have the potential to enhance P availability by solubilizing insoluble P forms. In this study, twenty-five PSB strains were initially isolated from acidic red and laterite soils in eastern India. Among them, ten bacteria formed a clear halo zone in Pikovskaya agar plates. The P releasing ability of these bacteria from different insoluble P sources was evaluated. The PSB strain AJ14, identified as <em>Enterobacter</em> sp., exhibited high P releasing capacity from tri-calcium phosphate, rock phosphate, ferric phosphate, and aluminum phosphate. Additionally, the strain demonstrated the ability to release zinc and potassium from insoluble sources. To improve the shelf life and efficacy of the PSB strain, liquid biofertilizer formulations were developed using polymeric additives such as polyvinylpyrrolidone, polyethylene glycol, glycerol, carboxymethyl cellulose, and tween 20. The liquid formulations showed enhanced survival and viability of the PSB strain compared to solid carrier-based biofertilizers. In a pot culture experiment with green gram plants, the liquid formulations significantly increased soil available P, dry matter yield, and P uptake by plants compared to the control and solid carrier-based biofertilizer treatment. Mitscherlich equation parameters indicated the greater effectiveness of the liquid formulations. The results suggest that liquid formulations of the PSB strain AJ14 can improve P utilization efficiency and plant growth in acidic red and laterite soils.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142532525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of structure of mycobacterial efflux pump protein Rv0194 and molecular dynamics simulation of the predicted structures","authors":"","doi":"10.1016/j.bcab.2024.103381","DOIUrl":"10.1016/j.bcab.2024.103381","url":null,"abstract":"<div><div>Tuberculosis is a major cause of morbidity and mortality primarily in developing nations. The rising number of cases of multidrug resistance and extensive drug resistance are a critical concern for the management of the condition. Even though new antibiotics are being developed, eventually there are also strains that are resistant to them. The evolution of resistance in <em>Mycobacterium tuberculosis</em> is significantly influenced by drug efflux caused by efflux pumps. Rv0194 is an important efflux pump associated with resistance to multiple drugs like beta lactam antibiotics like ampicillin and also erythromycin and novobiocin. The introduction of efflux inhibitors for Rv0194 could shorten the course of current therapy and improve the efficacy of second-line medications. Building a trustworthy molecular model of this efflux pump is the goal of this study. We created 3 models using different modeling tools. These models were then subjected to a 20 ns molecular dynamics simulation in a lipid bilayer. We find that the model built by Swiss Model shows the best results in molecular modeling and validation and the structure is also stable throughout the 20 ns MD simulations. Consequently, this model is reliable and can be used for further studies.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic profiling under poly (L-lactide) polymer degradation of the thermophilic filamentous bacterium Laceyella sacchari LP175 and its potential for polymer film hydrolysis","authors":"","doi":"10.1016/j.bcab.2024.103411","DOIUrl":"10.1016/j.bcab.2024.103411","url":null,"abstract":"<div><div>Polylactide or polylactic acid (PLA) is a hydrolysable polymer used in many applications including medical devices, agricultural films, and packaging materials. Biological methods that completely decompose biodegradable polymers are environmentally beneficial. <em>Laceyella sacchari</em> LP175, a thermophilic filamentous bacterium, had a high potential producing poly (L-lactide) (PLLA)-degrading enzyme which is characterized as a serine protease. This study demonstrated that this strain is an effective degrader of PLLA at a high temperature of 50 °C, which resulted in an increase in enzyme activity within the initial 24 h of incubation. The alterations in the physical and structural characteristics of the PLLA polymer film were verified to occur when incubated with a culture supernatant of this strain. Transcriptomic profiling revealed the differentially expressed genes of <em>Laceyella sacchari</em> LP175 under PLLA degradation, showing 289 and 296 up-regulated and down-regulated genes, respectively. Findings indicated that serine protease and transport-related genes played a key role in the biodegradation of PLLA in <em>Laceyella sacchari</em> LP175. The functions and metabolic pathway annotation of the enriched expression genes showed that propanoate metabolism, protein secretion, and membrane transportation were the main pathways induced when cells were grown in the presence of PLLA powder. Therefore, transcriptome data will be helpful in the development and manipulation of microbial systems to improve the degradation of biodegradable polymers.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142445312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced production, artificial intelligence optimized three-phase partitioning extraction, and in silico characterization of extracellular neutral Bacillus cereus proteinase","authors":"","doi":"10.1016/j.bcab.2024.103389","DOIUrl":"10.1016/j.bcab.2024.103389","url":null,"abstract":"<div><div>The present study applied atmospheric and room temperature plasma (ARTP) mutagenesis to improve <em>Bacillus cereus</em> strain for neutral proteinase production. The selected improved stable strain, Phe⁻Tyr^-ARTP-60-E demonstrated a 2.88-fold enhanced proteinase yield and 2.81-fold enhanced proteinase activity with shortened fermentation time. An artificial neural network-embedded genetic algorithm (ANN-GA) was employed to optimize the enzyme's three-phase partitioning (TPP) recovery using Python software. The optimized solution of the hybrid model recommended 38.05% wv⁻¹ (NH₄)₂SO₄, 1.0:2.0 crude extract/<em>tert</em>-butanol ratio, pH 5.84 at 23.4 °C, for the exclusive partitioning of proteinase in the intermediate phase, with 202% recovery, 13-fold purity with specific activity of 922 Umg⁻¹. Sensitivity analysis of the model revealed that enzyme recovery was most sensitive to crude extract/<em>tert</em>-butanol ratio with total sensitivity (ST) of 0.253 while purification factor was most sensitive to pH with an ST of 0.444. <em>In silico</em> analysis using Expasy ProtParam tool revealed that mutant proteinase had 3 amino acid substitutions; Val-to-Cys; Val-to-His and Ile-to-Lys, at positions 152, 288, and 292, respectively, and 39.2 kDa molecular weight, 6.42 isoelectric point, and 33.35 instability index. The 3-Dimensional structure of the enzyme predicted by ModWeb v r273 Server revealed 39% sequence identity with <em>Pseudoalteromonas lipolytica</em> thermolysin. The macromolecule increased transmittance in lysozyme-coated contact lenses by 99.6% in 45 min. We conclude that ARTP mutagenesis and ANN-GA optimized TPP are effective and efficient recalcitrant strain improvement and product recovery methods, respectively. Further genetic tools and analysis of the neutral proteinase-producing gene may be required for increased improvement toward sustainable application.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142323284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative growth, biochemical and antioxidative response of Glycine max and Sesamum indicum to MnS nanoparticles","authors":"","doi":"10.1016/j.bcab.2024.103386","DOIUrl":"10.1016/j.bcab.2024.103386","url":null,"abstract":"<div><div>Manganese is an important micronutrient that affects plant growth and development. This study proved that Manganese Sulfide nanoparticles (MnS NPs) are a better alternative to manganese salt (MnSO₄) for the growth of S<em>esamum indicum</em> and <em>Glycine</em> max under in vivo conditions. SEM, FTIR, EDX and, XRD revealed spherical, pure and, 8 nm size of MnS NPs. The addition of MnS NPs to the soil increased root and shoot length, number of branches, biomass, and protein contents in a dose-dependent manner up to 40 mg/kg in both <em>G.</em> max and S. <em>indicum</em>. Atomic Absorption Spectroscopy (AAS) showed bioaccumulation of Mn at 103.8 and 50.4 mg/kg DW in <em>G.</em> max root and shoot respectively at 80 mg/kg NPs soil and, 127.6 and 84.6 mg/kg DW in S<em>. indicum</em> root and shoot respectively at 80 mg/kg NPs soil. NPs application leads to the activation of antioxidant enzymes (SOD, POD) and the synthesis of secondary metabolites (total phenolics and flavonoids) in a concentration-dependent manner. The extract obtained from NPs treated plants showed higher total antioxidant capacity (TAC), total reducing power (TRP), and DPPH free radical scavenging activity. The hierarchical cluster analysis indicates 40 and 60 mg//kg soil NPs to be the stimulant of plant growth and alkaloid production. The results show that MnS NPs application influenced the plant biochemical profile, and enzymatic and non-enzymatic antioxidants in a dose-dependent manner. Therefore, MnS NPs can be novel elicitors to improve plant growth and biological contents.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142323282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green integrative large scale treatment of tannery effluent, CO2 sequestration, and biofuel production using oleaginous green microalga Nannochloropsis oculata TSD05: An ecotechnological approach","authors":"","doi":"10.1016/j.bcab.2024.103370","DOIUrl":"10.1016/j.bcab.2024.103370","url":null,"abstract":"<div><div>In this present investigation, the freshwater microalga <em>Nannochloropsis oculata</em> TSD05 was used to demonstrate multiforius environmental applications viz., bioremediation of tannery effluent, carbon sequestration, and green renewable biodiesel production from treated microalgal biomass. The microalga <em>Nannochloropsis oculata</em> TSD05 was exhibited unique heavy metal reduction capabilities and reduced significant amount of heavy metals viz., 96.62% of copper (Cu²⁺), 99.9% of chromium (Cr³⁺), 98.36% of zinc (Zn²⁺), 98.71% of cadmium (Cd²⁺), 97.96% of lead (Pb²⁺), 98.88% of Cobalt (Co²⁺), and 98.76% of nickel (Ni²⁺). The biosorption capacity rate (q_max) of heavy metals reduction and highest R² of 97.84% exhibited at 12 days of effluent treatment by Langmuir and Freundlich isotherm models. The phytotoxicity analysis was tested againt treated tannery effluent using <em>Vigna radiate, Sapindus muukorossi</em> seeds and 98.45%, 99.05% seeds were germinated. The <em>Nannochloropsis oculata</em> TSD05 microalga was utilized 98.45% of CO₂ by biofixation kinetics, 372 mg g⁻¹ of lipid, 3.65 mg mL⁻¹ of biomass and 4.64 mLg⁻¹ of biodiesel were produced. The produced biodiesel was confirmed and identification of 18 fatty acid methyl ester (biodiesel) compounds using GCMS. The recognition of 17 functional groups was identified using FTIR analysis and the microalga <em>Nannochloropsis oculata</em> TSD05 potential for sustainable and renewable green energy production. The potential future application of <em>Nannochloropsis oculata</em> TSD05 is to increase lipid production and biodiesel yield. Scaling up the bioremediation process can also validate these microalga's feasibility for use in wastewater treament. Enhancing heavy metal biosorption and carbon sequestration efficiency through genetic engineering could also maximize environmental benefits.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142327714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in redox status in raspberry (Rubus idaeus L.) fruit during ripening","authors":"","doi":"10.1016/j.bcab.2024.103380","DOIUrl":"10.1016/j.bcab.2024.103380","url":null,"abstract":"<div><div>The knowledge of the mechanisms affecting the process of berry fruit ripening is important, not only to correctly determine the appropriate date of harvest, at which the fruit is most palatable and characterized by adequate shelf-life stability, but also, to develop new strategies of regulating the ripening process before harvesting. It is recognized that berry fruit quality and its post-harvest shelf-life depend on the cellular redox homeostasis. We, therefore, decided to conduct a comprehensive analysis of the level of oxidative stress status in the raspberry fruit proper at different stages of fruit ripening, from green to overripe fruit. We assessed both the level of typical oxidative stress markers, i.e. ROS production, expression of antioxidant enzymes, degree of cell damage, as well as the expression of selected proteins involved in the energy, glutathione, and polyphenols metabolism. We found two stage-related peaks of ROS production. The first - in green fruit, which corresponded to the maximum expression of antioxidant enzymes (MnSOD, CAT), PARP-1, as well as proteins related to glutathione biosynthesis (GS, γ-GC), autophagy (ATG-8) and ubiquitination (UBQ-11). The second one, in the overripe fruit, was responsible for the intensification of oxidative modifications of cell components, i.e. lipids, proteins, and DNA, as well as the loss of low molecular-weight antioxidants. The fruit ripening process, in turn, was manifested by a strong increase in the expression of proteins involved in the biosynthesis of polyphenols (PAL, CHS), cellular respiration (ACO-1, Cyt-C-ox, ATPase), ethylene biosynthesis and signaling (SAMs, EIN-2) and increasing amounts of ABA.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142319607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}