Biocatalysis and agricultural biotechnology最新文献

{"title":"Advancement on dark fermentation of lignocellulosic biomass: unlocking potential through innovation and integration","authors":"Chahak Jain ,&nbsp;Monica Sachdeva Taggar ,&nbsp;Amanpreet Kaur ,&nbsp;Sahibleen Kaur ,&nbsp;Anu Kalia ,&nbsp;Sandip Gangil","doi":"10.1016/j.bcab.2026.103949","DOIUrl":"10.1016/j.bcab.2026.103949","url":null,"abstract":"<div><div>Hydrogen is considered an efficient fuel of the future, as it produces no carbon emissions and can be generated sustainably from renewable sources via biological processes. Biological hydrogen production is carried out by microorganisms through light-dependent mechanisms such as biophotolysis and photo-fermentation, and light-independent mechanisms such as dark fermentation. Each approach has its own merits and demerits in terms of energy efficiency and practicality; however, dark fermentation remains the primary focus of research owing to its cost-effectiveness and ability to degrade a wide spectrum of substrates. Nevertheless, low hydrogen yield and conversion efficiency remain major constraints of biological hydrogen production, hindering large-scale implementation. This review provides insights into lignocellulosic biomass as a potential feedstock for dark fermentation, along with its pre-treatment and the management of inhibitors generated during this process. Furthermore, it discusses advanced strategies to enhance biohydrogen productivity, including microbial immobilization techniques, metabolic engineering to improve strain efficiency, the application of nano-additives, integration of dark and photo-fermentation, as well as approaches for utilizing dark fermentation effluents for the production of value-added products.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103949"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of silver nanoparticles using Cinnamomum zeylanicum Blume post-distillation waste for antibacterial, antioxidant, anti-inflammatory and cytotoxicity activities","authors":"Chamini Madushika Panadura Lokuge ,&nbsp;Hasitha Dhananjaya Weeratunge ,&nbsp;Hondamuni Ireshika Chathurani De Silva ,&nbsp;Galabada Arachchige Sirimal Premakumara ,&nbsp;Pathmasiri Ranasinghe","doi":"10.1016/j.bcab.2026.103936","DOIUrl":"10.1016/j.bcab.2026.103936","url":null,"abstract":"<div><div>The synthesis of silver nanoparticles (AgNPs) using non-edible agricultural wastes offers a sustainable approach with promising applications. This study aimed to investigate a green approach for synthesizing AgNPs using cinnamon distillation waste (CDW). The formation of CDW-AgNPs was characterized and their antibacterial, antioxidant, cytotoxicity and anti-inflammatory effects were also investigated. The UV–Vis spectroscopy proved the characteristic absorption peak at <em>λ</em>_max 402 nm. XRD analysis revealed the presence of crystallographic planes characteristic of a face-centered cubic structure and the calculated crystalline size was 11.32 nm. TEM illustrated spherical shaped particles with diameters ranging from 13 nm to 23 nm. The EDS analysis showed the elemental configuration with a solid peak at 3 keV. DLS analysis revealed that the average size was 57.19 ± 0.39 nm, with moderate stability (−27.93 ± 1.37 mV) and dispersity (0.444 ± 0.003). CDW-AgNPs showed minimum growth inhibition against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> at 30 μg/mL and 70 μg/mL, respectively. CDW-AgNPs showed antioxidant activity with an inhibitory concentration (IC₅₀) of 41.53 ± 0.2 μg/mL. <em>In vitro</em> cell culture studies indicated that CDW-AgNPs were non-toxic to Vero cells at concentrations of 50–100 μg/mL. The results from the Arachidonate 5-Lipoxygenase (A5-LOX) inhibitory assay indicate that CDW-AgNPs, at a concentration of 146.63 ± 1.10 μg/mL, can inhibit A5-LOX activity by 50%. The IC₅₀ of CDW-AgNPs for <em>in vitro</em> nitric oxide (NO) inhibitory activity was 116.15 ± 0.01 μg/mL in lipopolysaccharide activated raw 264.7 macrophage cells. The obtained results indicate that CDW-AgNPs may be an effective cosmeceutical agent.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103936"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical profiling and chitosan nanoencapsulation of Betelvine (Piper betle L) leaf essential oil incorporated into hydrogel to combat multidrug-resistant β-lactamase and mecA-harbouring bacteria: In vitro and In vivo approach","authors":"Adrija Saha ,&nbsp;N. Harshitha ,&nbsp;Jyotibrata Mitra ,&nbsp;Varun Shamanna ,&nbsp;K.L. Ravikumar ,&nbsp;Sunil S. More ,&nbsp;Susweta Das Mitra","doi":"10.1016/j.bcab.2026.103941","DOIUrl":"10.1016/j.bcab.2026.103941","url":null,"abstract":"<div><div>Biopolymer-based nanoencapsulation offers a sustainable approach to enhance the efficacy of essential oils as phytopharmaceuticals against high-risk β-lactamase and <em>mecA</em>-positive multidrug-resistant (MDR) bacteria in clinical and veterinary settings. Essential oils from the leaves of two varieties of <em>Piper betel</em> L (Bangla and Sanchi) were extracted and phytochemically profiled to identify the more potent chemotype for biopolymer-based nanoencapsulation and topical formulation. GC-MS analysis showed that the Bangla variety (PBEO-B) contained higher eugenol (39.73 %) and exhibited stronger antibacterial activity (MIC: 0.3125–10 mg/mL) than the Sanchi variety (PBEO-S, eugenol-10.73 % and MIC up to 20 mg/mL). Mechanistic studies confirmed PBEO-B as a potent bacterial membrane disruptor, as confirmed by field emission scanning electron microscopy (FESEM) and leakage of intracellular macromolecules. PBEO-B also exhibited strong antioxidant activity (IC₅₀ of PBEO-B: 42.78 μg/mL). The nanoformulation F3 (PB-CNP) (chitosan:EO = 1:2) achieved the highest encapsulation efficiency (61.5 %) and loading capacity (40.7 %). FTIR and FESEM confirmed encapsulation and spherical morphology. Drug release followed biphasic kinetics (38 % in 2 h; 93 % over 96 h), fitting the Korsmeyer–Peppas mathematical model (R² &gt; 0.9), indicating pseudo-Fickian diffusion. Nanoencapsulation lowered effective doses by 1.25–10-fold against MDR strains. In <em>Galleria mellonella</em>, PB-CNPs improved survival (&gt;60 %) compared to antibiotic (∼40 %) and untreated groups. The PB-CNP hydrogel showed favourable properties (pH 5.4, spreadability ∼205 %, swelling ∼32 %) and prominent zone of inhibition (&gt;12 mm). In a murine MRSA-infected wound model, the hydrogel accelerated healing (∼99.6 % closure by day 21) and reduced bacterial load three-fold versus untreated. These results support chitosan-encapsulated PBEO-B as a promising phytopharmaceutical against MDR infections across sectors challenged by bacterial resistance.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103941"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of Trichoderma asperellum in a packed-bed reactor with antagonist activity against Fusarium","authors":"Karina Romero-Hernandez,&nbsp;Gabriela Sepúlveda-Jiménez,&nbsp;Mario Rodríguez-Monroy","doi":"10.1016/j.bcab.2025.103916","DOIUrl":"10.1016/j.bcab.2025.103916","url":null,"abstract":"<div><div>The fungus <em>Trichoderma asperellum</em> is a biological control agent against phytopathogenic fungi; however, the impact of substrate moisture on its metabolic and antagonistic responses during solid-state fermentation has been little studied, and it was the aim of this research. In addition, the antifungal performance of conidia produced in packed-bed reactors was compared with that of conidia produced in polypropylene bag cultures. Wheat and rice were used as substrates in both systems, and conidial yields were similar in magnitude (1.62 × 10⁸–2.06 × 10⁸ conidia g⁻¹). Still, with wheat, the mycelial growth and conidiation were more uniform, and the protein content and activity of amylases and cellulases were higher than in rice. In the packed-bed reactor with wheat, the highest conidial yield (4.56 × 10⁸ conidia g⁻¹) was obtained at 53 % substrate moisture, while protein content and enzyme activity were negatively affected by increased moisture. Conidia viability (97 %) and germination (91 %) were not affected by moisture content. The <em>T. asperellum</em> conidia produced in the packed-bed reactor inhibited <em>Fusarium</em> spp. mycelial growth by 72–85 %, while those conidia from bag systems inhibited the mycelial growth by 51–78 %. To the best of our knowledge, this is the first study to compare the antagonistic activity of <em>Trichoderma</em> conidia produced in two different configurations. Besides, the results demonstrate a relationship between moisture availability and protein metabolism and enzymatic activity in <em>T. asperellum</em>, under solid-state fermentation. The packed-bed reactor using wheat at 53 % moisture content is a potential alternative for producing <em>T. asperellum</em> conidia.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103916"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145941029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal properties of plant polyphenols-copper complex used as a wood preservative in rubber wood impregnation","authors":"Hong Yun ,&nbsp;Yuyou Liu ,&nbsp;Xiaoyan Wang ,&nbsp;Limin Wang","doi":"10.1016/j.bcab.2026.103918","DOIUrl":"10.1016/j.bcab.2026.103918","url":null,"abstract":"<div><div>To enhance the leaching resistance of copper-based preservatives, we developed an innovative wood preservative by complexing plant polyphenols with copper, achieving both superior antifungal efficacy and enhanced leaching resistance. The results showed that the grape-seed polyphenols complex copper preservative prepared at pH 2 had the best dispersion and antifungal properties, and the inhibition zones for white-rot fungus and brown-rot fungus were 50.77 mm and 43.40 mm, respectively. The retention of copper in the preservative-treated wood impregnated by one-step and two-step impregnation method was 10.02 % and 8.50 %, respectively. The Fourier transform infrared and scanning electron microscopy analyses showed the phenolic hydroxyl group of the preservative and the hydroxyl group of the wood cells cross-linked to form hydrogen bonds. After 14 days of anti-loss treatment, the fixation rate of copper in the preservative wood obtained by the two impregnation methods reached 96.49 % and 99.72 %, respectively, neither of the two types of preservative-treated wood was infected with mold. The antifungal properties and in-situ immobilization of the plant polyphenol-copper composite preservative prepared in this study have been verified, and this plant-derived composite preservative has great development potential.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103918"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145941028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic synthesis and fabrication of starch/PVA/CuO electrospun nanoscaffolds from Sargassum ilicifolium (brown marine macroalgae) extract for anticancer activity on HT-29 and MCF-7 cancer cells","authors":"Rajendraprasad Kunamalla ,&nbsp;Mani Panagal ,&nbsp;Chella Perumal Palanisamy","doi":"10.1016/j.bcab.2026.103923","DOIUrl":"10.1016/j.bcab.2026.103923","url":null,"abstract":"<div><div>This study presents the fabrication of starch/PVA electrospun nanoscaffolds incorporated with ultrasonically synthesized copper oxide nanoparticles (CuO NPs) derived from <em>Sargassum ilicifolium</em> extract, followed by an in-depth assessment of their anticancer potential. The biogenic CuO NPs were characterized using UV–Vis spectroscopy, which confirmed nanoparticle formation through distinct surface plasmon resonance (SPR) bands. Fourier-transform infrared spectroscopy (FTIR) analysis revealed functional group interactions between CuO NPs and the polymeric matrix, while X-ray diffraction (XRD) confirmed the monoclinic crystalline phase of CuO NPs and the semi-crystalline nature of the nanoscaffolds. Scanning electron microscopy (SEM) imaging demonstrated a well-organized, porous fibrous structure in the scaffolds, and Thermogravimetric analysis (TGA) indicated thermal stability up to 374 °C. Dynamic light scattering (DLS) revealed moderate colloidal stability, with an average particle size of 108 nm and a zeta potential of −18.3 mV. The anticancer efficacy of the algal extract, CuO NPs, and nanoscaffolds was evaluated against HT-29 colon cancer and MCF-7 breast cancer cells via the MTT assay. The nanoscaffolds exhibited superior cytotoxicity, with IC₅₀ values below 12.5 μg/mL for both cell lines, outperforming standalone CuO NPs (IC₅₀: 12.5–25 μg/mL) and the algal extract (IC₅₀: 25–50 μg/mL). These findings suggest that the starch/PVA/CuO-NPs nanoscaffolds enhance anticancer activity, likely due to improved cellular uptake or sustained release mechanisms. This study highlights the potential of these nanoscaffolds as a promising platform for cancer therapy, warranting further investigation into their mechanistic pathways and <em>in vivo</em> applications.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103923"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymatic hydrolysis of poly(lactic acid) by lipase and protease from Bacillus safensis","authors":"Chunwang Li ,&nbsp;Yujun Wang ,&nbsp;Hanzhong Liang ,&nbsp;Rui Cheng ,&nbsp;Yuanyuan Wang ,&nbsp;Zhanyong Wang","doi":"10.1016/j.bcab.2026.103930","DOIUrl":"10.1016/j.bcab.2026.103930","url":null,"abstract":"<div><div>The degradation of polylactic acid (PLA) by triacylglycerol lipase (TGL) and S8 family serine peptidase (S8SP), which were identified in <em>Bacillus safensis</em> and heterologously expressed in <em>Escherichia coli</em>, was investigated in this study. The degradation of PLA films by these two enzymes was investigated using scanning electron microscopy (SEM), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and gel permeation chromatography (GPC). TGL, S8SP, and their mixed enzymes resulted in PLA emulsion clarification and PLA film weight loss, and the mixed enzymes had better results. This implies that there is a synergistic effect between the two enzymes in the PLA degradation process. The degradation of the PLA films by both enzymes was due to surface erosion. PLA films treated with TGL exhibited irregular erosion on their surfaces, whereas the films treated with S8SP exhibited streak-like erosion. Enzymatic degradation leads to an increase in the number of hydrophilic groups on the surface, which in turn leads to an increase in the hydrophilicity of PLA films. In addition, the enzyme-treated PLA films showed a decrease in crystallinity and relative molecular weight, which was related to the degradation of the amorphous regions of PLA and the conversion of the crystalline regions into amorphous regions. The synergistic effect of TGL and S8SP in PLA degradation is an important reason for the ability of the source microorganism <em>Bacillus safensis</em> to effectively degrade PLA.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103930"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How germination conditions modulate the enzymatic activities, antioxidant potential and phenolic profile of chickpeas","authors":"Marcos Huann Bezerra Holanda,&nbsp;Ruann Janser Soares de Castro","doi":"10.1016/j.bcab.2026.103946","DOIUrl":"10.1016/j.bcab.2026.103946","url":null,"abstract":"<div><div>This study investigated the impact of germination conditions, including different photoperiods (dark, light, and alternating dark/light) and incubation times (12–96 h), on the germination rate, enzyme activities, phenolic compounds, and antioxidant properties of chickpeas. Germination consistently enhanced the activity of antioxidant enzymes (catalase, peroxidase, ascorbate peroxidase, superoxide dismutase) and hydrolytic enzymes (α-amylase, protease, lipase) as incubation progressed. Germination rates varied significantly (p ≤ 0.05) with conditions, ranging from 40 to 63 % under dark, 13–50 % under light, and 23–63 % under alternating regimes. Total phenolic content (TPC) increased across all treatments, from 2.31 mg GAE/g in non-germinated grains to 4.24 mg GAE/g (72 h, dark), 4.16 mg GAE/g (96 h, light), and 4.21 mg GAE/g (96 h, alternating). Antioxidant properties increased during germination compared to the control (non-germinated grains). For ABTS, DPPH, and FRAP, the increases were 48 %, 203 %, and 152 % in the dark; 59 %, 161 %, and 170 % in the light; and 56 %, 116 %, and 163 % under alternating dark/light conditions. HPLC analysis revealed gallic acid and catechin as the compounds with the highest increases during germination. These findings highlight germination as an effective strategy to enhance the antioxidant potential of chickpeas, with direct implications for the development of functional foods that align nutrition, health, and consumer demands. Overall, optimizing germination parameters and understanding the underlying biochemical mechanisms are crucial for tailoring the bioactive compound profile in legumes.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103946"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147397357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxic effects of Verbascum andrusii on lung cancer: Anti-elastase, anti-xanthine oxidase, antimicrobial, antioxidant properties, bioactive profile, and integrated in vitro and in silico assessments","authors":"Leyla Ercan ,&nbsp;Cemile Günbegi Çalişkan","doi":"10.1016/j.bcab.2026.103950","DOIUrl":"10.1016/j.bcab.2026.103950","url":null,"abstract":"<div><div><em>Verbascum andrusii</em> is an endemic species that grows in the Southeastern Anatolia Region. This study sought to figure out the total amount of phenolic molecules, the total amount of carotenoids, phenolic compounds, volatile compounds, antioxidant and antimicrobial activity, the inhibition effect on xanthine oxidase and elastase enzymes of <em>V. andrusii,</em> and to examine its cytotoxic effect on A549 (lung carcinoma) cells. For this, <em>V. andrusii</em>'s volatile and phenolic components were analyzed employing the HS-SPME technique and UHPLC-Orbitrap®-HRMS, respectively. Additionally, the antibacterial behavior by the disc diffusion method and the <em>in vitro</em> antioxidant capacity by four distinct techniques (DPPH, ABTS, CUPRAC, and DMPD) were assessed. Furthermore, the inhibitory effect of its on the enzymes elastase and xanthine oxidase was assessed both <em>in vitro</em> and <em>in silico</em>. Pharmacokinetic features and activities of bioactive components of <em>V. andrusii</em> were assessed <em>in silico</em> by ADME/T, molecular docking, and density functional theory (DFT). As a result, it was revealed that <em>V. andrusii</em> showed a cytotoxic effect on A549 cells (IC₅₀: 130.40 μg/mL) and had antioxidant, antimicrobial (on the microorganisms <em>E. coli, K. aerogenes, P. aeruginosa, C. albicans, S. aureus,</em> and <em>K.pneumoniae</em>), antiaging (antielastase IC₅₀: 450.10 μg/mL), and antihyperuricaemia (antixanthinoxidase IC₅₀: 239.02 μg/mL) properties. In addition, the effect of the components of this plant on the biological properties of the plant was evaluated by <em>in silico</em> studies. It was concluded that <em>in silico</em> investigations of diosmetin, luteolin, luteolin 7-rutinoside, apigenin, quinic acid, benzoic acid, anethole, and D-limonene, which are the main components of <em>V. andrusii</em>, confirmed the experimental studies.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103950"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol potential of estuarine bacterium Pseudoalteromonas sp. S-2: algicidal activity and mechanism against Microcystis aeruginosa","authors":"Ning Ding ,&nbsp;Qian-En Zhu ,&nbsp;Fan-Qiang Yan ,&nbsp;Zheng-Qi Wang ,&nbsp;Yu-Hao Song ,&nbsp;Chao Wang ,&nbsp;Ying Wang ,&nbsp;Yu-jiao Ma ,&nbsp;Pei-Ke Gao ,&nbsp;Ren-Jun Wang","doi":"10.1016/j.bcab.2026.103942","DOIUrl":"10.1016/j.bcab.2026.103942","url":null,"abstract":"<div><div>The proliferation of harmful cyanobacterial blooms, particularly <em>Microcystis aeruginosa</em>, poses significant threats to aquatic ecosystems and water security globally. Microbial control strategies have gained significant research interest as an environmentally sustainable and target-specific solution for <em>Microcystis</em> bloom management. This study investigated the algicidal activity and mechanism of <em>Pseudoalteromonas</em> sp. S-2, an estuarine bacterium, against <em>M. aeruginosa</em>. The results showed that at a concentration of 2 % (v/v), S-2 achieved 91.7 % mortality in <em>M. aeruginosa</em> within 96 h via an indirect mechanism. Physiological and molecular analyses revealed that S-2's cell-free supernatant induced oxidative stress, disrupted photosynthesis, carbohydrate metabolism, and protein synthesis in <em>M. aeruginosa</em>. Specifically, S-2 modulated antioxidant enzymes, caused membrane damage evidenced by increasing malondialdehyde accumulation and downregulating essential photosynthetic genes (<em>psaB</em>, <em>psbD</em>, and <em>rbcL</em>). Scanning electron microscopy (SEM) observations documented progressive morphological changes leading to cellular lysis in <em>M. aeruginosa</em>. Furthermore, the algicidal compounds exhibited remarkable stability across a wide range of temperatures (−80–120 °C) and pH values (4–12). Optimal environmental conditions for maximum algicidal efficacy were also identified. These findings establish <em>Pseudoalteromonas</em> sp. S-2 as a promising environmentally robust biocontrol agent for mitigating cyanobacterial blooms.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103942"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147397355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}