Biocatalysis and agricultural biotechnology最新文献

{"title":"Bioprocess optimization of L-asparaginase produced by Fusarium oxysporum CCM F-358: In vitro and in vivo characterization","authors":"Khaled A. Hassan,&nbsp;Yasmin M. Attia,&nbsp;Fatma I. Abou-Elazm,&nbsp;Asmaa Abdella,&nbsp;Mona T. Kashef","doi":"10.1016/j.bcab.2026.104269","DOIUrl":"10.1016/j.bcab.2026.104269","url":null,"abstract":"<div><div>L-asparaginase is approved for treating acute lymphoblastic leukemia, with associated toxicity and immunogenicity. Fungi are poorly characterized sources. We evaluated different fungal L-asparaginases and characterized the most active enzyme. Standard fungal species (n = 5) were screened for L-asparaginase and L-glutaminase activities (<em>Rhizopus oryzae</em> NRRL 3562<em>, Aspergillus niger</em> NRRL 3122, <em>Fusarium oxysporum</em> CCM F-358, <em>Trichoderma viride</em> DSM 63065<em>,</em> and <em>Trichophyton mentagrophytes</em> RCMB 21F001); <em>F</em>. <em>oxysporum</em> CCM F-358 produced the most active L-asparaginase (1.14U/mg) with undetected glutaminase activity. The maximum activity was recorded on the 6th day of fermentation at pH 6.2 and 30 °C, with agitation, using lactose and ammonium chloride as carbon and nitrogen sources. Multifactorial statistical optimization identified ammonium chloride and pH as the most significant factors affecting the activity. The enzyme purification increased activity by 9-fold, yielding 3.5% enzyme recovery and a specific activity of 198.86U/mg. The purified enzyme had a molecular weight of 40 kDa and K_m, V_max, and k_cat of 0.4 mM, 15.9 μmol mL⁻¹.min⁻¹ and 90.27 S^-1 with a K_cat/K_m of 2.25 x 10⁵ M⁻¹.S⁻¹. The enzyme had optimal activity at 37 °C and pH 8.6 and highest stability at 30 °C and pH 8. The enzyme had selective cytotoxicity, with IC₅₀ of 0.08, 0.17, 0.368, 0.14, 0.28, and 1.6 U/mL against MCF-7, T47D, Caco-2, HCT-116, THP-1, and HFB4. The in vivo anticancer activity was assessed in Ehrlich ascites carcinoma mouse model, resulting in tumor growth inhibition of 97.7%. In conclusion, this is the first characterization of the L-asparaginase from <em>F. oxysporum</em> CCM F-358 as a promising, selective anticancer enzyme.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104269"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149154588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic competence of gut-derived bacteria from the white grub Brahmina coriacea in insecticide degradation","authors":"Shubham Sharma,&nbsp;Abhishek Walia,&nbsp;Kuldeep Singh Verma,&nbsp;Suman Sanjta,&nbsp;Anila Badiyal,&nbsp;Prajjval Sharma","doi":"10.1016/j.bcab.2026.104284","DOIUrl":"10.1016/j.bcab.2026.104284","url":null,"abstract":"<div><div>White grubs of <em>Brahmina coriacea</em> are major subterranean pests in the north-western Himalayan region, where repeated insecticide applications often provide unsatisfactory control. The present study investigated the culturable gut bacteria of <em>B. coriacea</em> grubs to recover bacterial isolates capable of growing in the presence of insecticides and evaluate their insecticide-degrading capacity against bifenthrin, carbofuran, chlorpyrifos, and fipronil. Selective enrichment on insecticide-amended Minimal Salt media resulted in the isolation of seven bacterial strains with distinct colony morphologies. Phenotypic characterization followed by 16S rRNA gene sequencing revealed taxonomic affiliation with the genera <em>Pseudomonas</em>, <em>Klebsiella</em>, <em>Serratia</em>, and <em>Raoultella</em>. Growth kinetic assays demonstrated insecticide specific responses, with selected isolates achieving rapid proliferation (≈10⁹ CFU mL⁻¹) in the presence of their respective insecticides, even at elevated concentrations up to 200 ppm, suggesting physiological adaptation and tolerance. Reduction in insecticide residues was assessed by GC–MS, which revealed significantly greater degradation of all four insecticides after seven days in bacterial treatments than in uninoculated controls. These findings suggest that gut-associated bacteria may contribute to insecticide transformation and potentially influence insecticide persistence within the insect gut, although their contribution to reduced effectiveness in vivo remains to be established. Further studies are therefore warranted to elucidate the underlying degradation mechanisms and assess the potential relevance of these bacteria to sustainable pest management.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104284"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149200087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-engineered black soldier fly larval proteins immobilized on bio-silica nanoparticles for efficient lead remediation and alleviation of Pb stress in Phaseolus vulgaris","authors":"Layla Yousif Abdullah Al Hijab,&nbsp;Zarah I. Alzahrani,&nbsp;Fawzya AlOmari,&nbsp;Somia M. Elagib,&nbsp;Asma Algasem,&nbsp;Deyala M. Naguib,&nbsp;Dia Soltan","doi":"10.1016/j.bcab.2026.104244","DOIUrl":"10.1016/j.bcab.2026.104244","url":null,"abstract":"<div><div>This study reports the development of a novel nano-bioremediation system utilizing nano-engineered black soldier fly larval proteins (Nano-BSFP) for the removal of lead (Pb) and mitigation of Pb-induced stress in soil–plant systems. Larval proteins were extracted via an alkaline–acid method and immobilized onto bio-silica nanoparticles derived from rice straw. In batch experiments, Nano-BSFP achieved a maximum Pb removal efficiency of 97% at 2.5 g L⁻¹ and maintained approximately 80% of its adsorption capacity after 50 reuse cycles, indicating strong stability and reusability. Under greenhouse conditions, Nano-BSFP application enhanced soil water retention (48.39% after 8 days) and increased soil enzymatic activities, suggesting improved microbial functionality. In <em>Phaseolus vulgaris</em> L. subjected to Pb stress, treatment with Nano-BSFP reduced oxidative damage, stimulated antioxidant enzyme activities, and significantly improved plant biomass compared to untreated controls. These findings demonstrate the dual role of Nano-BSFP as an effective Pb adsorbent and a plant growth-promoting agent. To our knowledge, this is the first report on the use of insect-derived proteins immobilized on bio-silica nanoparticles, providing a sustainable strategy that integrates insect biotechnology, waste valorization, and nanotechnology for remediation of Pb-contaminated environments.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104244"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148938520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyaluronic acid production by polyurethane-immobilized Streptococcus zooepidemicus: process optimization, repeated-batch performance, and characterization of the recovered polymer fraction","authors":"Diane Rigo,&nbsp;Lucas Henrique do Nascimento,&nbsp;Carolina Elisa Demaman Oro,&nbsp;Emily Maximiano de Souza,&nbsp;Alexander Junges,&nbsp;Catia Santos Branco,&nbsp;Marina Rigotti,&nbsp;Marcos Rogério Dallago,&nbsp;Jamile Zeni","doi":"10.1016/j.bcab.2026.104245","DOIUrl":"10.1016/j.bcab.2026.104245","url":null,"abstract":"<div><div>This study investigated hyaluronic acid (HA) production by <em>Streptococcus zooepidemicus</em> immobilized in flexible polyurethane (PU) foam and characterized the recovered HA-enriched polymer fraction. Biomass loads of 4–10 g and the effects of temperature, agitation, and aeration were evaluated using the CTAB turbidimetric response. Under the central-point conditions (8 g biomass, 35 °C, 500 rpm, and 2 vvm), the mean apparent HA concentration was 0.471 g/L after 28 h, with individual values ranging from 0.445 to 0.508 g/L. Total organic carbon and total nitrogen decreased by 28% and 43%, respectively. Apparent kinetic parameters, calculated from CTAB-derived concentrations, included Y_P/S = 0.01484, mass productivity = 0.01486 g/L·h, and specific product formation rate = 0.00847 g/g·h. During repeated-batch operation, a measurable CTAB-reactive response persisted for up to 15 cycles; however, approximately 84% of the immobilized material was lost, and dense clump formation limited further analysis. Ethanol/NaCl precipitation yielded 56.65% apparent recovery of CTAB-reactive material. The recovered fraction showed no detectable cytotoxic effect on Vero cells up to 1000 μg/mL and measurable antimicrobial and ABTS responses, although these findings cannot be attributed exclusively to HA. FTIR showed functional groups consistent with an HA-containing fraction, and thermogravimetric analysis indicated a main degradation event near 280 °C. Because CTAB quantification was not fully validated in the fermentation matrix and molecular weight, rheological properties, and absolute purity were not determined, the results should be interpreted as preliminary. Overall, PU immobilization supported repeated-batch HA-associated production, but further analytical and process validation is required.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104245"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148938568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geographical origins influence on the chemical and antioxidant profiles of Moroccan sweet and bitter almonds","authors":"Sara El Bernoussi,&nbsp;Ihssan Boujemaa,&nbsp;Ahmed Hajib,&nbsp;Hasnae Choukri,&nbsp;Mohammed Amanullah,&nbsp;Abdelhakim Bouyahya,&nbsp;Agnese Santanatoglia,&nbsp;Giovanni Caprioli,&nbsp;Mohamed Tabyaoui,&nbsp;Hicham Harhar","doi":"10.1016/j.bcab.2026.104250","DOIUrl":"10.1016/j.bcab.2026.104250","url":null,"abstract":"<div><div>This study explores the impact of geographical origin on the physicochemical, phytochemical and antioxidant characteristics of sweet (<em>Prunus amygdalus</em>) and bitter (<em>P. amygdalus</em>) almonds from six Moroccan regions. Sweet almonds from Agadir stood out for their low moisture (2.94%), high oil yield (68.44%) and favorable lipid profile, including high levels of oleic acid (73.29%), β-sitosterol (82.91%) and α-tocopherol (93.14%). These samples also exhibited the highest phenolic (33.59 mg GAE/g) and flavonoid (11.17 mg QE/g) contents, alongside strong antioxidant capacity (DPPH IC₅₀ = 97.22 μg/mL; ABTS = 19.79 mg TE/g). All oil quality indicators met Codex standards. ANOVA and PCA analyses revealed significant effects of variety, region, and their interaction on oil composition and bioactivity. These findings highlight the importance of environmental factors in shaping almond quality and suggest that sweet almonds from Agadir are a promising source for high-value oil and antioxidant-rich formulations.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104250"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148997152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lower-molecular-weight yeast mannan polysaccharides beneficially modulate gut microbiota and fermentation profiles in broilers: An in vitro and in vivo assessment","authors":"Junwei Bao,&nbsp;Luqian Gong,&nbsp;Yuhua Zhao,&nbsp;Ran Wang,&nbsp;Jing Bai,&nbsp;Jinlong Liu","doi":"10.1016/j.bcab.2026.104241","DOIUrl":"10.1016/j.bcab.2026.104241","url":null,"abstract":"<div><div>Yeast-derived mannan polysaccharides (MPS) are recognized as promising prebiotics, yet the influence of their molecular weight (MW) on bioactivity remains poorly understood. This study evaluated the MW-associated prebiotic potential of three MPS fractions (high, medium and low-MW) through integrated <em>in vitro</em> and <em>in vivo</em> models. <em>In vitro</em>, while all MPSs exhibited high resistance to simulated gastrointestinal digestion, the lower-MW MPS (MPS-II and MPS-III) demonstrated more pronounced <em>in vitro</em> fermentation profiles, yielding higher lactic acid and short-chain fatty acids. Furthermore, 16S rRNA sequencing showed that the medium-MW MPS-II selectively enriched butyrate-producing <em>Clostridia</em>, while the low-MW MPS-III fostered <em>Lactobacillales</em>. Consistently, preliminary exploratory <em>in vivo</em> trials suggested that dietary supplementation with lower-MW MPSs (MPS-II and MPS-III) was associated with favorable changes in selected serum biochemical and immune parameters. These changes were accompanied by a beneficial reshaping of the cecal microbiota, characterized by a significant increase in beneficial bacteria like <em>Lactobacillus</em> and <em>Limosilactobacillus</em>, and a suppression of opportunistic pathogens <em>Proteobacteria</em>. These findings highlight the potential of tailored, lower-MW MPSs as high-efficacy prebiotics to enhance the gut health of broilers.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104241"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148948160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydroponics-driven controlled agriculture of small millets: A pathway toward circular economy and clean energy systems","authors":"Urvashi Dixit,&nbsp;Ankisha Vijay,&nbsp;Pooja Choudhary","doi":"10.1016/j.bcab.2026.104275","DOIUrl":"10.1016/j.bcab.2026.104275","url":null,"abstract":"<div><div>Global challenges related to food and energy security are intensifying due to declining arable land, soil degradation, freshwater scarcity, and increasing climatic variability. Small millets are widely regarded as climate-resilient, resource-efficient crops with significant potential to enhance food security in marginal environments; however, their utilization remains largely limited to conventional, soil-based, grain-oriented production systems. At the same time, the growing interlinkage between agriculture and energy demand underscores the need for integrated, sustainable approaches that address both food and energy security. This review provides a comprehensive synthesis of the current literature on small millets within the framework of controlled-environment agriculture, with a particular focus on hydroponic cultivation as a complementary biomass-oriented production platform for renewable bioenergy generation and circular bioeconomy applications. It further examines emerging concepts for integrating small-scale hydroponic millet systems with biomass valorization and bioelectrochemical technologies. Current evidence indicates that small millets, as C₄ plants, possess favourable physiological traits for efficient biomass production under controlled conditions, yet remain largely unexplored in hydroponic and energy-focused frameworks. Despite this potential, significant knowledge gaps persist in system optimization, biomass valorization, and the integration of millet-based hydroponic systems with bioelectrochemical technologies. Addressing these gaps through integrated technological optimization, life-cycle assessment, and techno-economic evaluation could enable multifunctional circular bioeconomy models that support sustainable biomass production, renewable energy generation, nutrient recycling, and wastewater reuse. Overall, hydroponic small millet-based bioenergy systems represent an emerging research pathway with potential to contribute to integrated food, energy, and water management, although their practical feasibility requires further experimental validation.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104275"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149154507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-scale structural and rheological characterization of Oxalis tuberosa flours: Impact of cultivar on starch architecture and viscoelastic gel stability","authors":"Adriana Rocío Chantre- López,&nbsp;José Fernando Solanilla-Duque,&nbsp;Diego Fernando Roa-Acosta,&nbsp;Adriana Carolina Flores-Gallegos,&nbsp;Margarita del Rosario Salazar-Sánchez,&nbsp;Raúl Rodriguez-Herrera","doi":"10.1016/j.bcab.2026.104268","DOIUrl":"10.1016/j.bcab.2026.104268","url":null,"abstract":"<div><div><em>Oxalis tuberosa</em> (Oca) is a tuber traditionally consumed by Andean communities; however, its nutritional and physicochemical attributes remain insufficiently characterized. This study investigated the compositional, structural, thermal, and rheological properties of flours from two cultivars (Mexico, OM; Colombia, OC) to establish structure–function relationships. Compared to potato (<em>Solanum tuberosum</em>), oca flour showed a protein content (5.43 % OC and 8.63 % OM), lipid (1.22 % OM and 2.96 % OC), and crude fiber (5.59 % OM and 5.82 % OC) contents, together with an enriched phytochemical profile including hydrolysable tannins (1.7 and 1.9 mg gallic acid equivalent/g, OM and OC, respectively), condensed tannins (1.9 and 4.2 mg catechin equivalent/g, OM and OC, respectively), hydroxycinnamic acids (e.g., caffeic acid), and catechin. Amylose content differed between cultivars (30 % in OM and 35 % in OC), whereas relative crystallinity was 16.39 % and 15.48 %, respectively. These differences influenced thermal behavior, as OC showed a higher gelatinization temperature (87.83 °C) than OM (77.52 °C), suggesting greater molecular organization within starch granules. Low starch digestibility (∼50 %) is linked to granule size, amylose organization, and residual components, suggesting potential as slowly digestible functional ingredient. Rheological analyses revealed cultivar-dependent differences in starch functionality. OC exhibited higher peak viscosity, whereas OM showed greater resistance to shear thickening and improved gel stability. Flour dispersions at 6 and 9 % (w/v) exhibited the most suitable rheological behavior for systems requiring stable viscosity. These results demonstrate that cultivar influences starch architecture and determines the technological functionality of <em>O. tuberosa</em> flour.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104268"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149154508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated use of Trichoderma yunnanense (NBRICRF_97) and biochar to reduce chromium accumulation in Spinacia oleracea L.","authors":"Priya Dubey,&nbsp;Aditi Roy,&nbsp;Sandhya Mishra,&nbsp;Mariya Naseem,&nbsp;Alvina Farooqui,&nbsp;Anju Patel,&nbsp;Pankaj Kumar Srivastava","doi":"10.1016/j.bcab.2026.104286","DOIUrl":"10.1016/j.bcab.2026.104286","url":null,"abstract":"<div><div>Chromium (Cr) contamination in agricultural soils poses a significant threat to crop productivity and food safety. This study investigated the efficacy of <em>Trichoderma yunnanense</em> (NBRICRF_97) and 0.5% Sugarcane Bagasse Biochar (SBC) for remediating Cr-contaminated soil and enhancing <em>Spinacia oleracea</em> growth, using four treatments: untreated Cr-contaminated soil (CR), <em>T. yunnanense</em> alone (CRF), SBC alone (CRB), and their combined application (CRFB). <em>T. yunnanense</em> exhibited plant growth-promoting attributes, including phosphate solubilization (239.69 μg mL⁻¹), auxin synthesis (189.66 μg mL⁻¹), and siderophore production. Experimental results demonstrated a substantial 7.2-fold increase in fungal population in the rhizosphere under CRFB. Exposure to Cr without amendments (CR) significantly inhibited plant growth, increased oxidative stress markers (proline, MDA), and upregulated antioxidant enzymes. In contrast, CRFB substantially enhanced plant growth, leading to a 59.08% increase in root length, 57.26% in shoot length, and a 134.74% rise in leaf area compared to CR. This was coupled with significant improvements in photosynthetic pigments and a reduction in stress indicators. Crucially, CRFB achieved an 80.86% reduction in soil Cr(VI) and a &gt;93% decrease in total Cr uptake in plant tissues compared to CR, reflected by low translocation (0.091) and bioconcentration factors. Consequently, the relative production index increased by 140.6% in CRFB, with minimal Cr accumulation (0.0034%) in crop biomass. Multivariate analyses revealed strong inverse correlations between Cr and plant growth, and positive associations with oxidative stress markers. This integrated biotic-abiotic strategy offers a sustainable approach for mitigating Cr toxicity and promoting crop productivity, with substantially reduced Cr accumulation in edible tissue.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104286"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149200088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of ionic liquid-compatible extremophilic laccases for biological lignin valorization","authors":"Can Liu,&nbsp;Paul Wolski,&nbsp;Jenny R. Onley,&nbsp;Hemant Choudhary,&nbsp;Kai Deng,&nbsp;Kshitiz Gupta,&nbsp;Trent R. Northen,&nbsp;Blake A. Simmons,&nbsp;Jian Shi","doi":"10.1016/j.bcab.2026.104270","DOIUrl":"10.1016/j.bcab.2026.104270","url":null,"abstract":"<div><div>Enzymatic lignin depolymerization, as a biomolecular engineering strategy, offers milder reaction conditions and improved selectivity compared to thermochemical methods; however, the low solubility of lignin in water remains a significant challenge. Certain biocompatible ionic liquids (ILs) with high lignin dissolution show promise in overcoming the solubility limitation and improving enzyme effectiveness. In this study, we evaluated the enzymatic activity and stability of five extremophilic laccases sourced from <em>Streptomyces lavendulae</em> REN-7 (Sl), <em>Thermus thermophilus</em> (Tt), <em>Haloferax volcanii</em> (Hv), <em>Trametes versicolor</em> (Tv) and <em>Myceliophthora thermophila</em> (Mt) in the presence of two selected ILs—ethanolamine acetate ([ETA][OAc]) and triethylammonium hydrogen sulfate ([Et₃NH][HSO₄]). The evaluation was conducted using the 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay and nanostructure-initiator mass spectrometry (NIMS). Results showed that the tested enzymes possess greater compatibility with [Et₃NH][HSO₄] than with [ETA][OAc], as demonstrated by stability tests in 5% (w/w) aqueous solutions. Notably, laccases Mt and Tt exhibit a slight activity increase of 10% and 8%, respectively, in the presence of 10% [Et₃NH][HSO₄] compared to the citrate buffer without IL, indicating strong compatibility with such IL under the tested conditions. Laccase Tt showed good compatibility with the ILs and efficiently transformed β-O-4′ and β-β′ model lignin dimers in NIMS assays, highlighting its potential to act on lignin substrates. This study provides a foundation for integrating enzyme engineering with solvent system design to advance lignin valorization and related applications in biological engineering.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"77 ","pages":"Article 104270"},"PeriodicalIF":4.6,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149200145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}