Biocatalysis and agricultural biotechnology最新文献

{"title":"In vitro evaluation of the bioherbicide effects of soluble metabolites derived from microbial phytopathogens on two weeds","authors":"Caliope Mendarte-Alquisira,&nbsp;Alejandro Alarcón","doi":"10.1016/j.bcab.2025.103748","DOIUrl":"10.1016/j.bcab.2025.103748","url":null,"abstract":"<div><div>The restriction on glyphosate-based herbicides has intensified the need for innovative and eco-friendly alternatives for weed management. In this context, phytopathogenic microorganisms represent a promising and sustainable source of bioactive compounds for herbicidal applications. This study explored the potential of soluble metabolites derived from selected phytopathogens to inhibit the growth of broad- and narrow-leaf weed species. Fungal strains including <em>Fusarium oxysporum</em> f. sp. <em>cubense</em> Race 1 (FocR1, CNRF-MIC17192), <em>Fusarium</em> spp. (LsF9, LsF11), <em>Sclerotinia sclerotiorum</em> (Ss4), <em>Sclerotium cepivorum</em> (Sc1), <em>Colletotrichum</em> sp., <em>Rhizoctonia solani</em>, and the bacterial strain <em>Pectobacterium carotovorum</em> were cultured in liquid media for 7 days. The resulting metabolites were filtered and applied to the foliage of two model plants: <em>Amaranthus hybridus</em> (broad-leaf) and <em>Festuca arundinacea</em> (narrow-leaf). Their phytotoxic effects were compared to a commercial glyphosate-based herbicide (Faena Fuerte® 360) over a 15-day period. Among the tested strains, CNRF-MIC17192 and Ss4 showed the highest efficacy on the broad-leaf plant, reducing chlorophyll content by approximately 65 % and biomass by 74 %. Conversely, metabolites from <em>P. carotovorum</em> exhibited the strongest effects on the narrow-leaf species, decreasing chlorophyll by 80 % and biomass by 70 %. Although the phytotoxicity of microbial metabolites was generally lower than that of chemical herbicide, the results underscore their potential as environmentally friendly bioherbicides for sustainable weed control. Preliminary results indicate that secondary metabolites contain enzymes with activity, such as cellulase and xylanase. In this context, <em>P. carotovorum</em> and Ss4 produced between ∼3.3 and ∼1.9 more activity than CNRF-MIC17192 strain.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103748"},"PeriodicalIF":3.8,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144917930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cymbopogon winterianus essential oil as an alternative source for producing terpenic esters with potential plant-protective antifungal activity using lipase immobilized on a lignin composite","authors":"Carlos Alberto Chaves Girão Neto ,&nbsp;Carolin Richter ,&nbsp;Isabel Cristina Medeiros Prasilde ,&nbsp;Kirley Marques Canuto ,&nbsp;Magda Elisa Turini da Cunha ,&nbsp;Bruno M. Moerschbacher ,&nbsp;Tigressa Helena Soares Rodrigues ,&nbsp;Maria Valderez Ponte Rocha","doi":"10.1016/j.bcab.2025.103746","DOIUrl":"10.1016/j.bcab.2025.103746","url":null,"abstract":"<div><div>Enzymatic esterification was evaluated as an alternative route to synthesis a new semi-synthetics compounds from <em>Cymbopogon winterianus</em> oil that can be applied as greener pesticides. The oil's major constituents were citronellal (40.32 % v/v), geraniol (26.77 % v/v) and citronellol (8.64 % v/v). Esterification catalyzed by lipase B from <em>Candida antarctica</em> immobilized on magnetic cashew apple bagasse lignin resulted in yields of 96.1 % for citronellyl butyrate and 96.9 % for geranyl butyrate after 24 h, significantly higher than the yields obtained by free enzyme, being 41.9 % and 52.2 %, respectively. The biocatalyst remained stable over five reaction cycles, yielding 88.1 % for citronellyl butyrate and 80.0 % for geranyl butyrate in the last cycle. The antifungal activity of citronellyl butyrate and geranyl butyrate esters against <em>Fusarium graminearum</em> were improved compared to its respective alcohols, showing a reduction of 66.8 % and 30.1 % in its half-maximal inhibitory concentration, respectively. The esterified citronella oil in the highest concentration evaluated (43,372 μg/mL) decreased by 87.5 % the disease incidence and thus the disease severity of <em>Botrytis cinerea</em> infection in <em>Phaseolus vulgaris</em> plants. Therefore, a pathway was proposed to obtain new value-added products which hold the potential to be applied as biopesticides for sustainable agriculture.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103746"},"PeriodicalIF":3.8,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing brewing industry byproducts for sustainable β-glucosidase production by recombinant Saccharomyces cerevisiae","authors":"Tamara van Coller,&nbsp;Chanté van der Walt,&nbsp;Shaunita H. Rose,&nbsp;Trudy Jansen","doi":"10.1016/j.bcab.2025.103743","DOIUrl":"10.1016/j.bcab.2025.103743","url":null,"abstract":"<div><div>The sustainable recirculation of industrial microbial waste biomass into value-added products offers an eco-friendly and cost-efficient approach to biocommodity production. In this study, we evaluated four expression plasmids: yEN1-Pccbgl1, yEN1d-Pccbgl1, yIN2-Pccbgl1 and yEN2-Pccbgl1 for β-glucosidase activity in <em>Saccharomyces cerevisiae</em>. The recombinant <em>S. cerevisiae</em> Y294[EN1d-Pccbgl1] strain containing the small high copy number plasmid displayed the highest level of enzyme activity at 190 U/g DCW. In contrast, the strain harbouring the integrative plasmid, <em>S. cerevisiae</em> Y294[IN2-Pccbgl1], displayed the lowest enzyme activity at 9.5 U/g DCW. The best performing <em>S. cerevisiae</em> Y294[EN1d-Pccbgl1] strain was subsequently assessed for enzyme production using brewery waste streams as feedstock. Cultivating the recombinant strain in defined media resulted in a maximum β-glucosidase activity of ∼1.0 U/mL comparable to the activity obtained when using 10 g/L <em>Komagataella pastoris</em> or commercial <em>S. cerevisiae</em> biomass as nitrogen sources. Notably, activity increased to ∼2.0 U/mL when brewers' spent yeast was used as the nitrogen source. Furthermore, when brewers' spent grains and brewers' spent yeast were used as carbon and nitrogen sources respectively, 1.46 U/mL of β-glucosidase activity was obtained. This is comparable to levels obtained using brewers’ spent grains in combination with commercial cellulases. These findings highlight a promising biotechnological approach: repurposing low-cost, carbohydrate-rich industrial waste streams in conjunction with microbial biomass waste for the economical production of recombinant enzymes. This strategy not only reduces production costs but also contributes to waste valorisation and circular bioeconomy initiatives.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103743"},"PeriodicalIF":3.8,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biofunctional and ecotoxicological assessment of Rosa indica derived nitrogen-doped Carbon Dots: A new frontier for sustainable nanomaterials","authors":"Hubza Ruatt Khan ,&nbsp;Humaira Yasmeen ,&nbsp;Muhammad Asad Tahir ,&nbsp;Asna Fatima Kiani ,&nbsp;Iqra Munir ,&nbsp;Unzish Qamar ,&nbsp;Sara Janiad ,&nbsp;Saher Mahmood","doi":"10.1016/j.bcab.2025.103744","DOIUrl":"10.1016/j.bcab.2025.103744","url":null,"abstract":"<div><div>Carbon Dots (CDs) have emerged as a promising material for a variety of therapeutic applications. Nitrogen doped <em>Rosa indica</em> (<em>R. indica</em>) fluorescent Carbon dots (N-RiCDs) were prepared by the utilization of pyrolysis method. Ultraviolet–visible, fourier transform infrared spectroscopy, X-ray diffraction, scanning microscope, energy-dispersive X-ray spectroscopy and raman spectroscopy revealed the optical and structural characteristics of the synthesized N-RiCDs. <em>In vitro</em> anti-oxidant assessment including free radical scavenging activity (DPPH assay) and phosphomolybdate assay showed EC₅₀ value of 45.28 μg/mL and 111.7 μg/mL respectively. <em>In vitro</em> anti-inflammatory assessment was performed by the utilization of egg albumin denaturation (EA) assay and anti-hemolytic assay. An EC₅₀ value of 36.05 μg/mL and 27.64 μg/mL was reported for protein denaturation and hemolysis protection capability respectively. Both anti-oxidant and anti-inflammatory activities were significantly increased in a dose-dependent manner. The photocatalytic dye degradation efficiency was observed higher (43.33 %–53 %) for phenol red under visible light irradiation while methylene blue degradation efficiency was observed higher (62.33 %–74.33 %) under UV irradiation. Toxicological profiling showed promising results including phytotoxicity and <em>in vitro</em> sperm motility assessment with significant minimal adverse effects. Low cytotoxicity was observed toward HepG2 cancer cells. <em>In vivo</em> studies revealed the mild acute toxicity in broiler chickens up to 10 mg/kg of N-RiCDs. This study proved that under controlled conditions, the N-RiCDs can be further scrutinized for potential clinical and environmental implementations.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103744"},"PeriodicalIF":3.8,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144919931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering precision defense: Fusaric acid-based wilt screening and chitosan nanoparticles for inducing resistance in sugarcane","authors":"Bhoomika M. Karamchandani ,&nbsp;Sunil G. Dalvi ,&nbsp;Samruddhi Kamble ,&nbsp;Shriram Mirajkar ,&nbsp;Mrunal Thorat ,&nbsp;Surekha K. Satpute","doi":"10.1016/j.bcab.2025.103730","DOIUrl":"10.1016/j.bcab.2025.103730","url":null,"abstract":"<div><div><em>Saccharum officinarum</em> (Sugarcane) is a leading cash crop due to its bioeconomic value. The crop is vulnerable to fungal pathogens, which necessitate the development of targeted and environmentally friendly fungicides. Current strategies embrace biotic origin materials in which the nanoparticles have presented several horizons for sustainable agriculture applications. This work investigates the efficacy of foliar application of fungal chitosan-origin chitosan nanoparticles (FCHNPs) on sugarcane plants challenged with mycotoxin- Fusaric acid (FA). To circumvent the menace associated with the direct spread of pathogen - <em>Fusarium</em> spp. to the environment, the mycotoxin was sprayed on the sugarcane leaves. FCHNPs were synthesized using fungal chitosan extracted from <em>Cunninghamella echinulata</em> NCIM 691. The <em>in-vivo</em> assays demonstrated the potency of FCHNPs at 100 μg/ml concentration on sugarcane var. Co86032. The FCHNPs-treated leaves showed high chlorophylls at 100 μg/ml, indicating substantial improvement in the photosynthetic activity. The relative electrolyte leakage was reduced in FCHNPs-treated plants, indicating a reduction in membrane damage. Additionally, the lower malondialdehyde (MDA) content indicated lower oxidative stress with the treatments. The activity of the enzymes that contribute in the defence system of plants, viz polyphenol oxidase, chitinase and β-1,3-glucanase was substantially enhanced in treated settlings. Overall, <em>in-vivo</em> studies supported the efficacy of FCHNPs in sugarcane challenged with FA. Consequently, this research demonstrates a proactive and sustainable approach using the foliar application of FCHNPs to enhance sugarcane resilience against phytopathogens in a sustainable way. This strategy may help in determining the resistance to <em>Fusarium</em> at an early stage of varietal developments in sugarcane.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103730"},"PeriodicalIF":3.8,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iron sulfide nano-meerschaum composite (M-FeS) prolonged the release of bioavailable iron: Optimising accessibility, release kinetics and uptake in direct seeded rice under alkaline stress","authors":"Khushboo Rathour ,&nbsp;Anjali Sidhu","doi":"10.1016/j.bcab.2025.103740","DOIUrl":"10.1016/j.bcab.2025.103740","url":null,"abstract":"<div><div>Iron bioavailability is a persistent challenge for sensitive crops such as rice which is usually exacerbated by electrochemical reactivity and rapid oxidation of iron, further aggravated under alkaline-stress. Sustainable alternatives are mandated over traditional practices to augment iron uptake, under which, the study leveraged the nanotechnological and chemical modification of iron as iron sulfide nano-meerschaum composite (<em>M-</em>FeS). <em>M-</em>FeS was prepared, characterized and statistically optimized by Response surface methodology (RSM) in alkaline loamy sand soil (pH 8.7) for maximum extractable Fe, subjected to varying organic matter (1.82–5 %), iron doses (5–500 μg/g) and incubation times (0–30 days). The soil incubation at an optimized dose of <em>M-</em>FeS (473.34 μg/g) presented extractable Fe at 54.72 %, with 39.31 % in bioavailable ferrous form, sustained over 29.47 days. In contrast, FeSO₄, optimized at 495.04 μg/g, achieved 64.1 % extractable Fe over 8.9 days with only 13.5 % in ferrous form. The Higuchi model was the best fit of release kinetic model indicating a Fickian diffusion-controlled mechanism facilitating sustained ferrous ion availability in alkaline soils. Direct-seeded rice (DSR) grown under pot studies in <em>M-</em>FeS treated soil showed significantly higher corrected iron uptake of 15.38 % in shoots and 28.56 % in roots relative to 5.45 % and 6.85 %, respectively, under FeSO₄ treatment on day 29. <em>M-</em>FeS, thus, represented a prolonged-release iron fertilizer, promoting sustainable agriculture through improved iron delivery in DSR under alkaline stress.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103740"},"PeriodicalIF":3.8,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144903006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical profiling of Satureja horvatii Šilić, Lamiaceae extracts: Experimental and molecular docking aspects","authors":"Emilia I. Gligorić,&nbsp;Nikolina Kirćanski,&nbsp;Milica Vidić,&nbsp;Branislava D. Teofilović,&nbsp;Nevena N. Grujić-Letić","doi":"10.1016/j.bcab.2025.103727","DOIUrl":"10.1016/j.bcab.2025.103727","url":null,"abstract":"<div><div><em>Satureja horvatii</em> Šilić is an endemic species of the Balkan Peninsula traditionally used in many tea blends, yet insufficiently explored. This study aimed to evaluate the ethanol extracts and herbal infusion of this species in terms of phenolic composition, antioxidant activity, and anti-HIV potential of its constituents. The total phenolic and flavonoid content of herbal infusion and 70 % (v/v) ethanol extracts was determined spectrophotometrically, while detailed chemical characterization by high pressure liquid chromatography coupled with diode array detection (HPLC-DAD). Antioxidant activity was evaluated by the ability of extracts to neutralize 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. The antiviral potential of compounds was tested against cysteine-cysteine chemokine receptor type 5 (CCR5) by molecular docking. The major bioactive components of the extracts were rutin (30.90–69.40 mg/g dry extract (de)), rosmarinic (13.10–45.30 mg/g de), and p-coumaric acid (14–35.50 mg/g de). The extracts exhibited significant DPPH radical scavenging potential (IC₅₀ values ranging from 4.63 to 9.94 μg/mL). In the molecular docking study, flavonoid derivates – naringenin, epicatechin, quercetin; and chlorogenic acid among the phenolic acids had a high binding affinity toward CCR5 and interacted with key residues at the active site of the target receptor. <em>S. horvatii</em> extracts show promising antiviral potential and are worthy of further research in discovering novel anti-HIV therapy.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103727"},"PeriodicalIF":3.8,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144889396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical profiling, biological activities, and in silico molecular docking studies of water hyacinth (Pontederia crassipes mart.) aerial part extracts","authors":"Widad Ben Bakrim ,&nbsp;Amine Ezzariai ,&nbsp;Fadoua Karouach ,&nbsp;Nawal Merghoub ,&nbsp;Badreddine Drissi ,&nbsp;Mulugeta Kibret ,&nbsp;Mohamed Hafidi ,&nbsp;Lamfeddal Kouisni","doi":"10.1016/j.bcab.2025.103733","DOIUrl":"10.1016/j.bcab.2025.103733","url":null,"abstract":"<div><div>Water hyacinth (<em>Pontederia crassipes</em> Mart.) has received a growing attention for its valorization to convert it from a threat to an added value plant with a plethora of biological properties. This study investigates the bioactive phytochemicals and <em>in vitro</em> biological activities of several extracts of <em>P. crassipes</em> collected from Lake Tana, Ethiopia. The research also includes an <em>in silico</em> molecular docking analysis to support the findings.</div><div>The bioactive compounds were evaluated through preliminary phytochemical tests, LC MS/MS, and GC-MS analysis for chemical profiling. The antioxidant activity was performed by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), chelation iron, and FRAP assays. <em>In vitro</em> anti-inflammatory activities were evaluated by lipoxygenase inhibition, protease inhibition, and albumin denaturation assays. As results, the ethanol and water extracts revealed their richness in polyphenols and flavonoids evidenced by the phytochemical contents. GC-MS analysis of n-hexane extract revealed the identification of several phytosterols, where stigmasterol and β-sitosterol dominated the extract. LC-MS/MS analysis identified 34 compounds in the aerial parts of the ethanolic extract, predominantly comprising polyphenols as well as organic and phenolic acids. Ethanol and water extracts showed good antioxidant potential. Moreover, the hexane extract of the plant exhibited modest anti-inflammatory activity by the inhibition of lipoxygenase and protease. Furthermore, no cytotoxic effect was observed on WS1 and HaCaT cells at higher concentrations for the different extracts. The major identified compounds were further analyzed using <em>in silico</em> molecular docking studies to assess their binding affinity with enzymes such as lipoxygenase, ovalbumin, and Bovine Serum Albumin. The activity prediction suggest that these compounds possess anti-inflammatory properties. Additional validation through molecular dynamics (MD) simulations suggests that emodin-sulfate and stigmasterol could form stable complexes with lipoxygenase and Bovine Serum Albumin, respectively. Furthermore, the pharmacokinetic properties of these compounds were evaluated using SwissADME, highlighting their potential as promising drug candidates.</div><div>Thus, the findings of this study emphasized the potential use of <em>P. crassipes</em> biomass and its high value-added products in the pharmaceutical industry and medical research.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103733"},"PeriodicalIF":3.8,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoagrochemicals: Ensuring sustainable productivity through nutrient and pest management in strawberry (Fragaria × ananassa Duch.)","authors":"Lakhwinder Singh ,&nbsp;Ramesh Kumar Sadawarti ,&nbsp;Diksha Thakur ,&nbsp;Shailesh Kumar Singh ,&nbsp;Anis Ahmad Mirza","doi":"10.1016/j.bcab.2025.103737","DOIUrl":"10.1016/j.bcab.2025.103737","url":null,"abstract":"<div><div>Strawberry cultivation is commercially viable worldwide due to its high market demand, as it bears fruits with high nutritional value and appealing taste. Though a wide range of high-yielding varieties are available, their dependence on growing conditions restricts their commercial sustainability. To ensure the sustainable production of strawberries, it is essential to work on the management of the nutritional and biotic stressors through effective utilization of nanoagrochemicals. The new generation of nanofertilizers can be a potential source of plant nutrition, which could have high efficacy in delivering the essential elements to plants for balanced growth and yield. Encapsulating the biopesticides in nanoformulations can enhance the effectiveness of pesticides, leading to the minimum use of synthetic pesticides for ecosystem safety. The current review examines the transformative impact of nanoagrochemicals on strawberry crop cultivation, specifically emphasizing the innovative utilization of nanofertilizers and nanopesticides. It investigates the potential advantages of enhanced nutrient management, improved soil quality, and augmented crop yields by using nanoagrochemicals.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103737"},"PeriodicalIF":3.8,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144903005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional and metabolomic profiling of Artemisia dracunculus and Allium ampeloprasum: A combined UPLC-QTOF-MS/MS and GC-MS approach","authors":"Hanieh Rahmani ,&nbsp;Javad Asili ,&nbsp;Zahra Tayarani-Najaran ,&nbsp;Mourad Kharbach ,&nbsp;Karen Segers ,&nbsp;Yvan Vander Heyden ,&nbsp;Azin Fattahi ,&nbsp;Seyedeh Faezeh Taghizadeh ,&nbsp;Abolfazl Shakeri","doi":"10.1016/j.bcab.2025.103731","DOIUrl":"10.1016/j.bcab.2025.103731","url":null,"abstract":"<div><div>Tarragon <em>(Artemisia dracunculus</em> L.) and wild leek <em>(Allium ampeloprasum</em> L.) are commonly used in traditional Iranian cuisine. The current study aimed to evaluate the total phenolic contents, the antioxidant and cytotoxic activities of <em>A. dracunculus</em> and <em>A. ampeloprasum</em> extracts and their chemical profiling-based on GC-MS as well as UPLC–PDA–ESI–QTOF–MS/MS analysis. The butanolic extract of <em>A. dracunculus</em> displayed the most potent antioxidant activity in DPPH and <em>β</em>-carotene bleaching methods with IC₅₀ values of 37.6 and 33.18 μg/mL, respectively. None of the extracts demonstrated cytotoxicity against MCF7, SW48, and NIH 3T3 cell lines. Results from GC-MS analysis of the essential oil (EO) revealed that phenyl propanoids (76.6 %) and oxygenated sesquiterpenes (30.4 %) were the most abundant components of the EO of <em>A. dracunculus</em> and <em>A. ampeloprasum,</em> respectively. Additionally, the UPLC–PDA-ESI–QTOF–MS/MS analysis revealed high amounts of rutin (54.28 mg/g) and quercitrin (24.60 mg/g) in the butanolic extract of <em>A. dracunculus,</em> and ethyl acetate extract of <em>A. ampeloprasum</em>, respectively, which may contribute to the high antioxidant activity observed in both plants.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"68 ","pages":"Article 103731"},"PeriodicalIF":3.8,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144865884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}