Biocatalysis and agricultural biotechnology最新文献_第5页

Optimizing bioethanol production from hassawi rice straw with Aspergillus sp. NAS51 cellulosic enzyme and in silico homology modeling 利用黑曲霉 NAS51 纤维素酶和硅同源建模优化哈萨维稻草的生物乙醇生产

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-07-19 DOI: 10.1016/j.bcab.2024.103328

{"title":"Optimizing bioethanol production from hassawi rice straw with Aspergillus sp. NAS51 cellulosic enzyme and in silico homology modeling","authors":"","doi":"10.1016/j.bcab.2024.103328","DOIUrl":"10.1016/j.bcab.2024.103328","url":null,"abstract":"<div><p>Researchers' attention has been greatly focused on the use of lignocellulosic wastes to produce bioethanol and biogas due to the depletion of non-renewable energy resources. In our effort to find a potent cellulase-producing fungal strain, the fungus NAS51 was isolated among eight isolates from a sponge collected from the Red Sea, Jeddah, and selected as it displayed the highest cellulase activity including (FP-ase, CMC-ase, and β-glucosidase enzymes) at levels of 3.13 U/ml, 2.52 U/ml, and 0.69 U/ml, respectively. The fungus was identified morphologically and genetically by sequencing its 18SrRNA gene as <em>Aspergillus</em> sp. NAS51. The cellulase activity of <em>Aspergillus</em> sp. NAS51 was optimized and maximum enzyme production was obtained at initial pH7, temp 30<sup>o</sup>C, incubation period 11 days, moisture content 70%, urea as a nitrogen source, and K2HPO4 (2 g/L). The crude cellulases from strain NAS51 were characterized, and results showed that the enzyme is stable over a wide pH range (6–10), with peak activity at pH 7.0 and 40 °C. The cellulase gene has been sequenced and the protein 3D structure was generated via in silico homology modeling. Determination of binding sites and biological annotations of the constructed protein was carried out via COACH and COFACTOR based on the I-TASSER structure prediction. To reach the maximum enzyme hydrolysis, the rice straw collected from Al-Ahsa, Kingdom of Saudi Arabia was pretreated with NaOH 1.5% to remove lignin and to enhance the saccharification process by Cellulase. The saccharified product was measured using HPLC, fermented by S. cerevisiae and the bioethanol yield produced from the fermentation was 0.454 mL ethanol/g fermentable sugars. This study indicates the potential application of fungal enzymes such as cellulase enzymes in biofuel generation and waste management.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141842065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis, characterization, and wound healing activity of alginate-based polymeric nanoparticles loaded with stingless bee honey 负载无刺蜂蜂蜜的藻酸盐基聚合物纳米粒子的合成、表征和伤口愈合活性

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-07-18 DOI: 10.1016/j.bcab.2024.103329

{"title":"Synthesis, characterization, and wound healing activity of alginate-based polymeric nanoparticles loaded with stingless bee honey","authors":"","doi":"10.1016/j.bcab.2024.103329","DOIUrl":"10.1016/j.bcab.2024.103329","url":null,"abstract":"<div><p>Nanobiotechnology could aid in developing honey-based natural wound healing agents. In the present work, stingless bee honey (SBH) loaded alginate nanoparticles (H-ALG NPs) were formulated with an ionic crosslinking method and thoroughly characterized. The average size of the H-ALG NPs was observed to be 312 ± 4.32 nm, with 0.112 ± 0.04 PDI and - 21.2 ± 0.29 mV Zeta potential. TEM and SEM imaging confirmed the presence of spherical-shaped NPs. Encapsulation efficiency and loading capacity were observed to be 84.74% and 23.12%, respectively. Further, H-ALG NPs showed dose-dependent radical scavenging activity against DPPH with IC<sub>50</sub> 23.20 compared to 35.99 mg/mL for SBH, suggesting antioxidant potential. The H-ALG NPs were also tested for cytotoxicity on a human dermal fibroblast cell line and exhibited low toxicity with a higher IC<sub>50</sub> than SBH (970.07 vs. 755.67 μg/mL; <em>P</em> < 0.001). H-ALG NPs also significantly promoted the migration of fibroblasts as indicated by scratch assay. The qPCR analysis for gene expression demonstrated that H-ALG NPs potentially promote the production of proinflammatory mediators (i.e., TNF-α, IL-6, IL-1β and IL-8) in fibroblasts, which play a crucial role in wound healing, albeit at lower levels compared to SBH. The mouse wound model confirmed that H-ALG NPs accelerated wound healing (<em>P</em> < 0.05) with improved histological characteristics. These findings highlight H-ALG NPs as a potential natural and effective therapeutic agent for wound management and advocate for further exploration of the antioxidant-based medicinal properties of SBH.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141960815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bio fabrication of microalgae mediated nanoparticles: An insight into its mechanism, characterization and applications 微藻介导纳米粒子的生物制造：深入了解其机理、特性和应用

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-07-18 DOI: 10.1016/j.bcab.2024.103330

引用次数: 0

Differential expression of superoxide dismutase gene in response to magnesium salt in oryza sativa (L) plants grown in arsenic infested soil of gangetic West Bengal 在西孟加拉邦恒河流域砷污染土壤中生长的旱獭（Oryza sativa (L)）植物超氧化物歧化酶基因对镁盐的差异表达

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-07-18 DOI: 10.1016/j.bcab.2024.103327

{"title":"Differential expression of superoxide dismutase gene in response to magnesium salt in oryza sativa (L) plants grown in arsenic infested soil of gangetic West Bengal","authors":"","doi":"10.1016/j.bcab.2024.103327","DOIUrl":"10.1016/j.bcab.2024.103327","url":null,"abstract":"<div><p>Arsenic is a matter of concern across the globe especially for the countries like India where a good number of people depend on rice as the staple source of carbohydrate. Arsenic has lot of deleterious effects on the plant and through food chain gets accumulated to human being and is highly toxic and carcinogenic for human health. The present research is a biomarker based study to understand the difference in arsenic induced toxicity with application of Mg-salt. The primary objective was to see the difference in the expression of Superoxide dismutase gene. In the electrophoresis, sample with Mg-salt treatment gave less dense band compared to the control one. This was also corroborated with the reduction in antioxidant and stress biomarkers like, phenol, malonaldehyde, flavonoid, superoxide dismutase content. On the contrary chlorophyll content showed an increase of more than twice and protein content 1.7 times in Mg-salt treated plants compared to the control. Interestingly root arsenic content showed almost two times increased value and shoot arsenic content showed decrease of 1.5 times in Mg-salt treated plants. It can be deciphered that Mg might have acted as a reason for less translocation of arsenic in case of paddy plant.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141845751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structure-based in-silico identification of natural compounds as potential inhibitors of ran GTPase for breast cancer treatment 基于结构的硅内鉴定天然化合物，将其作为治疗乳腺癌的 Ran GTPase 潜在抑制剂

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-07-17 DOI: 10.1016/j.bcab.2024.103325

{"title":"Structure-based in-silico identification of natural compounds as potential inhibitors of ran GTPase for breast cancer treatment","authors":"","doi":"10.1016/j.bcab.2024.103325","DOIUrl":"10.1016/j.bcab.2024.103325","url":null,"abstract":"<div><p>Genomic instability segregation significantly contributes to the cellular mechanisms that affect the transition from normal to neoplastic cell proliferation, which enhances the ability of cancer cells to spread to distant sites and cause secondary growth, leading to cancer development. Mutated chromosome segregation can result from various factors, such as compromised centromere duplication, and disrupted assembly of the mitotic spindle. Due to metastasis, cancer is known to be associated with an increased mortality rate among patients. Hence, to suggest the development of more effective treatment strategies, it is essential to identify biomolecular and genetic markers that can serve as prognostic and predictive indicators in the progression of breast cancer. For instance, Ran GTPase (1K5G) has been recognised as a potential contributor to breast cancer. Ran, a small GTPase, plays a role in various cellular processes. The primary objective of the study was to assess the potential therapeutic advantages of natural compounds against breast cancer, with a specific focus on the Ran GTPase protein. The approach involved a virtual screening method to identify the most efficient compounds from the <em>N</em>P-lib database at the MTiOpenScreen website against 1K5G. Following the screening process, the top three compounds were selected for molecular docking along with a co-crystallized GUANOSINE-5′-DIPHOSPHATE (GDP) inhibitor serving as a reference compound. In the active site of 1K5G against the reference inhibitor GDP, each compound showed significant docking energy between −9.1 and −8.9 kcal/mol. Further, the study also used molecular dynamic simulation (100 ns) to analyze the stability and physical movements of atoms and molecules. The compounds within this group can disrupt interactions involving the Ran GTPase protein within cells.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141841806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thalictrum foliolosum DC against aphid, Aphis craccivora Koch and mealybug, Planococcus lilacinus Cockerell: A potential bioinsecticide Thalictrum foliolosum DC 可防治蚜虫 Aphis craccivora Koch 和蚧壳虫 Planococcus lilacinus Cockerell：一种潜在的生物杀虫剂

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-07-17 DOI: 10.1016/j.bcab.2024.103326

{"title":"Thalictrum foliolosum DC against aphid, Aphis craccivora Koch and mealybug, Planococcus lilacinus Cockerell: A potential bioinsecticide","authors":"","doi":"10.1016/j.bcab.2024.103326","DOIUrl":"10.1016/j.bcab.2024.103326","url":null,"abstract":"<div><p><em>Aphis craccivora</em> and <em>Planococcus lilacinus</em> are significant plant virus carriers and severe pests of legumes, fruits, and commercial crops. Inappropriate use of inorganic pesticides for the management of pests leads to resistance, detrimental to natural enemies of pests and the environment. In this study, methanolic root extract, different fractions, and isolated compounds from <em>Thalictrum foliolosum</em> DC were investigated for their pesticidal and enzyme inhibition potential against target pests. The findings revealed that thalidasine and berberine were most effective against <em>A</em>. <em>craccivora</em> (LD<sub>50</sub> = 0.43–0.47 μl/insect), followed by <em>n</em>-butanol fraction (LD<sub>50</sub> = 1.37 μl/insect). Similarly, the aqueous fraction was more promising against <em>P</em>. <em>lilacinus</em> (LD<sub>50</sub> = 0.24 μl/insect) as compared to jatrorrhizine and thalidasine (LD<sub>50</sub> = 0.60–0.64 μl/insect). Further UHPLC-PDA-based analysis revealed that chloroform fraction showed higher thalidasine (37.86 ± 0.923 mg/g) and berberine (37.76 ± 0.398 mg/g) content, whereas berberine (36.06 ± 0.045 mg/g) also quantified in dominating amount in <em>n</em>-butanol fraction, together with jatrorrhizine (23.74 ± 0.177 mg/g). In reproduction inhibition assay, the chloroform and methanol extract showed a higher inhibition rate at 10 000 mg/L (98.69–99.46%). Additionally, methanolic extract significantly inhibited the detoxifying enzymes (glutathione <em>S</em>-transferase and acetylcholinesterase) in target pests. Therefore, based on field bio efficacy data of extract/fractions and compounds, the lead (s) can be employed further for the preparation of botanical insecticide. The present finding provides the first report on simultaneous quantification of three isoquinoline alkaloids by UHPLC-PDA and the bio-insecticidal potential of <em>T. foliolosum</em>.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141848426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical profile, nutritional and medicinal value of Nigella sativa 黑麦草的植物化学成分、营养和药用价值

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-07-14 DOI: 10.1016/j.bcab.2024.103324

{"title":"Phytochemical profile, nutritional and medicinal value of Nigella sativa","authors":"","doi":"10.1016/j.bcab.2024.103324","DOIUrl":"10.1016/j.bcab.2024.103324","url":null,"abstract":"<div><p>This review article provides phytochemical profiling, nutritional and medicinal importance of <em>Nigella sativa</em> (NS). Its seeds are rich in thymol, thymoquinone, thymohydroquinone, dithymoquinone, organic acids, resins, reducing sugars, metarbin, poisonous glucoside, proteins, minerals, carbohydrates, lipids and vitamins. Its sees serve as an important nutritional source and healthy dietary supplement for human beings and animals to improve their growth performance in cattle and chickens. The use of NS helps in fighting against various infections including inflammation, cancer, asthma, fungus, lowering blood sugar and heart-related diseases. Intake of NS seeds (NSS) results in significant lowering of systolic blood pressure, body weight, hip ratio, waist fasting blood sugar, uric acid, SGOT, SGPT, diastolic blood pressure, triglyceride and serum testosterone. Its seeds have carminative, appetite, digestive and stimulant properties and are useful for treating flatulence, anorexia, indigestion and gas production in the digestive tract. They improve liver and stomach functions and are effective against nausea, stomach cramps, diarrhea, leprosy, freckling, alopecia, eczema of the skin, and seasonal viral illnesses. Chewing of its seeds is useful in controlling the antimicrobial activities in the oral cavity. NS improves digestive juice secretion and prostaglandin production, stimulates menstrual flow, reduces breast milk abnormalities and increases milk production. NSS oil is effective for vitiligo patients and skin re-pigmentation, especially in the pubic area, face, and hands. Its seeds stimulate urination and are used to remove kidney stones.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141693837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient production and enhanced accumulation of Valerenic acid in Valeriana officinalis: Early identification of high-performing hairy root clones and Pioneering use of hydrogen peroxide as an elicitor 缬草中缬草烯酸的高效生产和强化积累：高效毛根克隆的早期鉴定和过氧化氢作为激发剂的开创性使用

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-07-14 DOI: 10.1016/j.bcab.2024.103319

{"title":"Efficient production and enhanced accumulation of Valerenic acid in Valeriana officinalis: Early identification of high-performing hairy root clones and Pioneering use of hydrogen peroxide as an elicitor","authors":"","doi":"10.1016/j.bcab.2024.103319","DOIUrl":"10.1016/j.bcab.2024.103319","url":null,"abstract":"<div><p><em>Valeriana officinalis</em> (valerian) roots and rhizomes possess a long history of medicinal use due to their sedative, antiepileptic, and anticonvulsant properties. Valerenic acid, a bioactive sesquiterpene with therapeutic potential, is present in limited quantities within these tissues. This study explores the application of hairy root cultures for enhanced valerenic acid production. Hairy root induction was attempted on valerian leaves and petioles using three <em>Rhizobium rhizogenes</em> strains (ATCC15834, A4, and MSU440) across three culture media (Murashige and Skoog (MS), Gamborg's B5 (B5), and Schenk and Hildebrandt (SH)). A non-destructive imaging system and periodic analyses were employed to identify superior hairy root clones exhibiting increased branching frequency. Leaf explants co-cultured with <em>R. rhizogenes</em> strain ATCC15834 yielded the most promising clones, characterized by the highest dry weight (1.03 mg) and valerenic acid content (0.384 mg/g dry weight) when grown in a half-strength SH liquid medium. Following strain and media optimization, the impact of 50 mM hydrogen peroxide as an elicitor on valerenic acid production was investigated. This treatment resulted in a significant 1.76-fold increase in valerenic acid accumulation compared to the control group at the first day post-treatment. This approach presents a valuable strategy for the early identification of high-yielding hairy root lines. Moreover, the utilization of hydrogen peroxide, a safe and cost-effective elicitor, offers a rapid method for enhancing valerenic acid production in the selected superior clone. This study establishes a promising platform for the sustainable production of valuable plant compounds within both research and industrial settings.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141630261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review of the advances in detoxification strategies of lignocellulosic hydrolysate for bio-based succinic acid production 木质纤维素水解物用于生物基琥珀酸生产的解毒策略进展综述

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-07-14 DOI: 10.1016/j.bcab.2024.103323

{"title":"A review of the advances in detoxification strategies of lignocellulosic hydrolysate for bio-based succinic acid production","authors":"","doi":"10.1016/j.bcab.2024.103323","DOIUrl":"10.1016/j.bcab.2024.103323","url":null,"abstract":"<div><p>Succinic acid is a valuable platform chemical that can be produced from renewable resources, such as lignocellulosic biomass (LCB). The utilization of LCB to produce succinic acid involves a pretreatment step that results in a hemicellulosic hydrolysate. This hydrolysate is enriched with sugars, but it also contains inhibitory compounds at levels that could impede the growth of microorganisms due to their toxicity and can hamper fermentation process. Hydrolysate detoxification is a critical step in the production of succinic acid from lignocellulosic biomass, aiming to remove inhibitory compounds that hinder fermentation efficiency. This review evaluates hydrolysate production from lignocellulosic biomass towards succinic acid production. A comprehensive discussion was done on detoxification methods, including adsorption techniques, evaporation, overliming, neutralization, membrane filtration, solvent extraction, enzymatic, microbial, and combined detoxification approaches. Each method's principles, effectiveness, advantages, and limitations are discussed. Furthermore, future perspectives in hydrolysate detoxification for succinic acid production are outlined, focusing on tailored detoxification protocols, novel detoxification agents and technologies, biotechnological approaches, process optimization and scale-up, technoeconomic and environmental analysis, and mathematical and computational modeling. Overall, this review highlights the importance of continued research and development in hydrolysate detoxification to advance sustainable succinic acid production from lignocellulosic biomass.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141700542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recycling biotoxic green walnut husks through mushroom cultivation and impact of these wastes on yield and nutritional composition of Pleurotus spp. 通过蘑菇栽培回收利用生物毒性青核桃壳以及这些废物对假蘑菇产量和营养成分的影响

IF 3.4

Biocatalysis and agricultural biotechnology Pub Date : 2024-07-08 DOI: 10.1016/j.bcab.2024.103322

{"title":"Recycling biotoxic green walnut husks through mushroom cultivation and impact of these wastes on yield and nutritional composition of Pleurotus spp.","authors":"","doi":"10.1016/j.bcab.2024.103322","DOIUrl":"10.1016/j.bcab.2024.103322","url":null,"abstract":"<div><p>The aim of the study was the bioconversion of green walnut husk (GWH) to nutrimental foods through mushroom cultivation technology. Two isolates of <em>Pleurotus ostreatus</em> and three isolates of <em>Pleurotus eryngii</em> cultivated in four different growing media supplemented different ratios of GWH were investigated for cultivation cycle, yield, biological efficiency (BE), fruiting body properties and nutritional composition of <em>Pleurotus</em> spp. In the study, poplar sawdust (PS) was used as a base substrate, and GWH were added at the ratios of 9:1, 8:2 and 7:3 to prepare the growing media. The control medium was prepared using PS only. Although increasing the GWH content led to prolonged pinning and flushing times, the addition of GWH at 10–20% ratios had a positive impact on mushroom performance. The yield and BE (%) of <em>P. ostreatus</em> and <em>P. eryngii</em> isolates ranged between 120.2 and 230.0 g/kg and 40.1–74.2% BE and 121.8–222.5 g/kg and 40.6–76.2% BE, respectively, depending on the growing medium used. Moreover, the fruitbodies of both strains of <em>P. ostreatus</em> demonstrated significant increases in protein and fat content when produced on substrates containing GWH, especially at a 20% ratio in the growing medium. These results indicate that GWH, an agricultural waste that poses a risk to the environmental health, could be successfully used in the cultivation of both oyster mushroom species.</p></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141638110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0