Functional and metabolomic profiling of Artemisia dracunculus and Allium ampeloprasum: A combined UPLC-QTOF-MS/MS and GC-MS approach

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology Pub Date : 2025-08-19 DOI:10.1016/j.bcab.2025.103731

Hanieh Rahmani , Javad Asili , Zahra Tayarani-Najaran , Mourad Kharbach , Karen Segers , Yvan Vander Heyden , Azin Fattahi , Seyedeh Faezeh Taghizadeh , Abolfazl Shakeri

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引用次数: 0

Abstract

Tarragon (Artemisia dracunculus L.) and wild leek (Allium ampeloprasum L.) are commonly used in traditional Iranian cuisine. The current study aimed to evaluate the total phenolic contents, the antioxidant and cytotoxic activities of A. dracunculus and A. ampeloprasum extracts and their chemical profiling-based on GC-MS as well as UPLC–PDA–ESI–QTOF–MS/MS analysis. The butanolic extract of A. dracunculus displayed the most potent antioxidant activity in DPPH and β-carotene bleaching methods with IC₅₀ values of 37.6 and 33.18 μg/mL, respectively. None of the extracts demonstrated cytotoxicity against MCF7, SW48, and NIH 3T3 cell lines. Results from GC-MS analysis of the essential oil (EO) revealed that phenyl propanoids (76.6 %) and oxygenated sesquiterpenes (30.4 %) were the most abundant components of the EO of A. dracunculus and A. ampeloprasum, respectively. Additionally, the UPLC–PDA-ESI–QTOF–MS/MS analysis revealed high amounts of rutin (54.28 mg/g) and quercitrin (24.60 mg/g) in the butanolic extract of A. dracunculus, and ethyl acetate extract of A. ampeloprasum, respectively, which may contribute to the high antioxidant activity observed in both plants.

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黄花蒿和苦参的功能和代谢组学分析：UPLC-QTOF-MS/MS和GC-MS联合方法

龙蒿（Artemisia dracunculus L.）和野韭菜（Allium ampeloprasum L.）通常用于传统的伊朗菜肴中。本研究采用GC-MS和UPLC-PDA-ESI-QTOF-MS /MS分析方法，对龙骨草和蛇梨提取物的总酚含量、抗氧化活性和细胞毒活性进行了评价，并对其化学性质进行了分析。在DPPH和β-胡萝卜素漂白方法中，丁醇提取物的抗氧化活性最强，IC50值分别为37.6和33.18 μg/mL。提取物对MCF7、SW48和NIH 3T3细胞系均无细胞毒性。气相色谱-质谱分析结果显示，苯丙素含量最高（76.6%），氧合倍半萜含量最高（30.4%）。此外，UPLC-PDA-ESI-QTOF-MS /MS分析显示，龙骨草丁醇提取物和蛇梨乙酸乙酯提取物中分别含有高含量的芦丁（54.28 mg/g）和槲皮苷（24.60 mg/g），这可能是两种植物抗氧化活性较高的原因。

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来源期刊

Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

7.70

自引率

2.50%

发文量

308

审稿时长

48 days

期刊介绍： Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.