Biocatalysis and agricultural biotechnology最新文献_第6页

Nicotinamide-functionalized nanobiochar enhances iron/zinc bioavailability and wheat growth through controlled micronutrient delivery 烟酰胺功能化纳米炭通过控制微量营养素的输送，提高铁/锌的生物利用度和小麦生长

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2026-02-01 Epub Date: 2026-01-13 DOI: 10.1016/j.bcab.2026.103933

Amin Janinejad , Naser Karimi , Zahra Souri , Leila Norouzi , Shahryar Sasani , Muhammad Farooq

{"title":"Nicotinamide-functionalized nanobiochar enhances iron/zinc bioavailability and wheat growth through controlled micronutrient delivery","authors":"Amin Janinejad , Naser Karimi , Zahra Souri , Leila Norouzi , Shahryar Sasani , Muhammad Farooq","doi":"10.1016/j.bcab.2026.103933","DOIUrl":"10.1016/j.bcab.2026.103933","url":null,"abstract":"<div><div>This study reports the synthesis and evaluation of a nicotinamide-functionalized nanobiochar (NBNZF) as a nano-engineered fertilizer to improve iron (Fe) and zinc (Zn) nutrition in wheat. Nanobiochar derived from wheat straw was functionalized with nicotinamide and loaded with ZnFe<sub>2</sub>O<sub>4</sub> nanoparticles to enable controlled micronutrient delivery. Material characterization using UV–Vis spectroscopy, FTIR, XRD, and SEM confirmed successful functionalization, nanoparticle incorporation, and a porous nanoscale structure. The bioefficacy of NBNZF was assessed in two wheat cultivars (Amin and Baran) using a completely randomized design across a range of application concentrations. Compared with conventional Fe–Zn–NA salts, NBNZF exhibited a markedly slower and more sustained release of Fe and Zn, resulting in significantly enhanced root uptake. Germination and early growth responses showed strong concentration dependence: low-dose NBNZF significantly enhanced germination, root elongation, chlorophyll accumulation, whereas higher doses suppressed germination but mitigated reductions in shoot growth. At optimal concentrations, NBNZF increased root length by up to 46 % and chlorophyll <em>a</em> content by up to 72 %, while consistently promoting Fe and Zn accumulation in both roots and shoots relative to nanobiochar-free controls. These responses were accompanied by improved physiological performance, indicating more efficient micronutrient utilization. Overall, integrating nicotinamide as both a chelating and metabolic modulator within a nanobiochar carrier enables controlled Fe/Zn delivery and improved plant uptake, offering a promising nano-enabled strategy for enhancing micronutrient homeostasis and early growth in wheat.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103933"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic engineering and scale-up of Klebsiella michiganensis for high-level 2,3-butanediol production and agricultural application 代谢工程与高产2,3-丁二醇生产及农业应用的密歇根克雷伯菌规模化

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2026-02-01 Epub Date: 2026-01-24 DOI: 10.1016/j.bcab.2026.103948

Kyeong Ho Kim , Ji Won Cha , Eun Seo Song , Jaewoo Son , Min-Ho Jo , Baek-Rock Oh , Sun Chang Kim , Ki Jun Jeong

{"title":"Metabolic engineering and scale-up of Klebsiella michiganensis for high-level 2,3-butanediol production and agricultural application","authors":"Kyeong Ho Kim , Ji Won Cha , Eun Seo Song , Jaewoo Son , Min-Ho Jo , Baek-Rock Oh , Sun Chang Kim , Ki Jun Jeong","doi":"10.1016/j.bcab.2026.103948","DOIUrl":"10.1016/j.bcab.2026.103948","url":null,"abstract":"<div><div>2,3-Butanediol (2,3-BDO) is a versatile platform chemical with applications ranging from industrial solvents and fuel additives to agricultural biostimulants and biopesticides. However, its industrial deployment requires robust microbial production at large scale with high titer, yield, and productivity, as well as clear demonstration of functional value. In this study, we engineered <em>Klebsiella michiganensis</em> (strain KH001) by eliminating major by-product pathways (<em>ldhA</em>, <em>ackA-pta</em>, <em>poxB</em>) and enhancing the <em>budABC</em> operon via promoter replacement, thereby efficiently redirecting carbon flux toward 2,3-BDO synthesis. The engineered strain achieved 115.1 ± 2.5 g/L of 2,3-BDO with a productivity of 3.84 ± 0.18 g/L/h in a 5 L bioreactor, and maintained strong performance during scale-up, reaching 104.2 g/L and 2.31 g/L/h productivity in a 500 L pilot bioreactor. Beyond production, the cell-free fermentation broth containing predominantly <em>meso</em>-2,3-BDO exhibited remarkable agricultural efficacy. Soil application of the broth suppressed root-knot nematode (<em>Meloidogyne incognita</em>) infection in tomato plants, achieving control comparable to or exceeding a commercial nematicide. In addition, treated plants showed improved growth under nematode stress and enhanced drought tolerance, maintaining shoot biomass and leaf turgor under water deficit conditions. These findings establish the dual role of fermentation-derived 2,3-BDO as both a biopesticide and a biostimulant, addressing both biotic and abiotic stress.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103948"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aspergillus flavus and Colpomina sinousa mixture act as biocatalyst to improve growth, fruit yield and salinity resistance of Brassica napus for sustainable agriculture 黄曲霉与红曲霉混合作为生物催化剂可促进甘蓝型油菜的生长、产量和耐盐性，实现可持续农业

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2026-02-01 Epub Date: 2026-01-17 DOI: 10.1016/j.bcab.2026.103944

Hira Wahid , Humaira Gul , Mamoona Rauf , Asma Tabassum , Aliya Rehman , Muhammad Arif , Muhammad Hamayun , Hatem E. Semary , Zainul Abideen

{"title":"Aspergillus flavus and Colpomina sinousa mixture act as biocatalyst to improve growth, fruit yield and salinity resistance of Brassica napus for sustainable agriculture","authors":"Hira Wahid , Humaira Gul , Mamoona Rauf , Asma Tabassum , Aliya Rehman , Muhammad Arif , Muhammad Hamayun , Hatem E. Semary , Zainul Abideen","doi":"10.1016/j.bcab.2026.103944","DOIUrl":"10.1016/j.bcab.2026.103944","url":null,"abstract":"<div><div>Plant survival under salinity conditions can be nourished by applying biocatalyst (algal-fungal consortia) as biotechnological tool to improve plant salinity resistance and use of saline land and water to address food security. <em>Colpomina Sinousa</em> and endophytic fungi <em>Aspergillus flavus</em> were used as a long-term solution to enhance the growth and physiological output of <em>Brassica napus</em> plants grown at 125 mM NaCl. <em>A. flavus</em> was used at the rate of (60 ml per seedling) with 4 % <em>C. Sinousa</em> aqueous extract (CAE) application on 35, 70 and 100 days after sowing. Salinity reduced chlorophyll <em>a and b</em>, RWC, LWL, IAA, GA<sub>3</sub>, water potential, osmotic potential, potassium, calcium, magnesium, K<sup>+</sup>/Na<sup>+</sup>, Ca<sup>2+</sup>/Na<sup>+</sup> and Mg<sup>2+</sup>/Na<sup>+</sup> ratios, while increased salicylic acid, H<sub>2</sub>O<sub>2</sub>, lipid peroxidation, proline, phenols, flavonoids, terpenoids, carbohydrates, lipids, protein, lycopene, beta carotene, tannins, and ascorbate peroxidase, ABA, salicylic acid, ascorbic acid and sodium compared to non-saline media. Interestingly, application of <em>A. flavus</em> and <em>C. Sinousa</em> in combination enhanced growth, improved biochemical attributes and essential nutrients uptake with decline in sodium accumulation that increased yield under normal and saline stress. It can be concluded that the application of <em>A. flavus</em> and <em>C. Sinousa</em> as new formulation of bio-stimulants causes pronounced improvement in vegetative and reproductive yield and improved salt tolerance of <em>B. napus.</em> Using theses biostimulant is a sustainable approach as plant biofertilizers and bio enhancer to increase crop productivity specially in saline environment.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103944"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomic analysis of carotenoids biosynthesis by Sphingomonas morindae sp. NBD5 森叶鞘单胞菌NBD5合成类胡萝卜素的代谢组学分析

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2026-02-01 Epub Date: 2025-12-30 DOI: 10.1016/j.bcab.2025.103917

Kun Lin , Chao Liu , Yang Liu , Qianqian Xu , Xiaoyu Cao , Yijun Yin , Hai Yan

引用次数: 0

Novel commensal rhizobacteria isolated from field-grown canola plants 从油菜中分离的新型共生根瘤菌

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2026-02-01 Epub Date: 2025-12-23 DOI: 10.1016/j.bcab.2025.103899

Christopher Blake , Storme de Scally , John Pascoe , Atharva Bhide , Aysha L. Sezmis , Perran L.M. Cook , Vanessa N.L. Wong , Harsh Raman , Michael J. McDonald

{"title":"Novel commensal rhizobacteria isolated from field-grown canola plants","authors":"Christopher Blake , Storme de Scally , John Pascoe , Atharva Bhide , Aysha L. Sezmis , Perran L.M. Cook , Vanessa N.L. Wong , Harsh Raman , Michael J. McDonald","doi":"10.1016/j.bcab.2025.103899","DOIUrl":"10.1016/j.bcab.2025.103899","url":null,"abstract":"<div><div>Plant growth-promoting rhizobacteria have the potential to reduce reliance on synthetic fertilizers. However, the rhizosphere-associated microbiota of canola (<em>Brassica napus</em>) remains understudied despite the crop's global significance. In this study, we isolated and characterized 12 bacterial strains from canola roots, to better understand the diversity and potential agricultural benefits of the canola microbiome. Using a combination of long- and short-read whole-genome sequencing, we generated high-quality genome assemblies of all 12 bacterial species. Genomic analysis revealed genes linked to nitrogen fixation and other potential functional capabilities relevant to plant growth. Growth in nitrogen-deficient media, ammonium production asssays, and acetylene reduction assays, support potential nitrogen fixation traits. Although bacterial inoculation did not significantly enhance early seedling development under nitrogen-limited conditions, continuous coculture with bacteria did not negatively impact plant performance, suggesting a commensal relationship between these isolates and their canola host. This work expands current knowledge of the canola microbiome by identifying commensal microbes closely associated with canola roots for synthetic-community design aimed at improving canola productivity.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103899"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145941027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Volatile profile of Calicotome spinosa (L.) Link. revealed by SPME-GC-MS: Phytotoxic and insecticidal activities supported by in silico analysis 花椰菜（calictomome spinosa）的挥发性特征链接。SPME-GC-MS显示：植物毒性和杀虫活性得到硅分析的支持

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2026-02-01 Epub Date: 2026-01-12 DOI: 10.1016/j.bcab.2026.103931

Roukia Zatout , Ouided Benslama , Chaima Zatout , Saliha Kaouache , Stefania Garzoli

{"title":"Volatile profile of Calicotome spinosa (L.) Link. revealed by SPME-GC-MS: Phytotoxic and insecticidal activities supported by in silico analysis","authors":"Roukia Zatout , Ouided Benslama , Chaima Zatout , Saliha Kaouache , Stefania Garzoli","doi":"10.1016/j.bcab.2026.103931","DOIUrl":"10.1016/j.bcab.2026.103931","url":null,"abstract":"<div><div>In the search for eco-friendly alternatives to synthetic agrochemicals, the volatile organic compounds (VOCs) of <em>Calicotome spinosa</em> (L.) Link, a Mediterranean shrub valued for its medicinal and ecological significance, were investigated. Molecular identification using ITS sequencing showed high similarity to reference material (accession PX285687). The study aimed to characterize the plant's volatiles through solid-phase microextraction coupled with gas chromatography–mass spectrometry (SPME-GC–MS) and to evaluate their phytotoxic and insecticidal properties, supported by in silico molecular docking. SPME-GC–MS analysis identified 20 VOCs, with hexahydrofarnesyl acetone (33.6 %), dihydroactinidiolide (13.4 %), trans-β-ionone (12.7 %), farnesane (6.0 %), and 3,5-di-tert-butylphenol (5.6 %) as major constituents. Bioassays showed that hexane and dichloromethane extracts exhibited strong, dose-dependent phytotoxicity against <em>Lens culinaris</em>, significantly inhibiting germination and seedling growth. Insecticidal assays demonstrated potent fumigant activity: dichloromethane extract caused up to 100 % mortality of <em>Stegobium paniceum</em> and 90 % of <em>Ephestia kuehniella</em> within 72 h, while n-hexane showed slightly lower efficacy. Residual toxicity confirmed consistent mortality across treatments. Molecular docking indicated strong binding of hexahydrofarnesyl acetone and 3,5-di-tert-butylphenol to acetylcholinesterase (AChE) and the octopamine receptor (Oamb) in <em>Drosophila melanogaster</em>, suggesting a dual neurotoxic mode of action. Overall, <em>C. spinosa</em> VOCs represent promising natural candidates for sustainable bioherbicide and bioinsecticide development.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103931"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing humification and valorization of sawdust and manure via bioaugmentation and moisture control in composting 通过生物强化和水分控制优化木屑和粪肥在堆肥中的腐殖质化和增值

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2026-02-01 Epub Date: 2026-01-13 DOI: 10.1016/j.bcab.2026.103928

Nedio Ricardo Rogoski , Sabrina Pinto Salamoni , Gustavo Graciano Fonseca

{"title":"Optimizing humification and valorization of sawdust and manure via bioaugmentation and moisture control in composting","authors":"Nedio Ricardo Rogoski , Sabrina Pinto Salamoni , Gustavo Graciano Fonseca","doi":"10.1016/j.bcab.2026.103928","DOIUrl":"10.1016/j.bcab.2026.103928","url":null,"abstract":"<div><div>The objective of this study was to produce and characterize compounds obtained from sawdust and swine and bovine waste, as well as their combinations. Three composting strategies using solid bovine manure (SBM), solid swine manure (SSM), raw wood sawdust (RWS), and decomposed wood sawdust (DWS) were evaluated over a period of 28–60 days to optimize organic waste valorization. In Experiment 1, mixtures containing 10 % RWS achieved rapid moisture equilibration and reached mesophilic temperatures (∼35 °C) within 15 days, whereas 100 % SBM or SSM remained below ambient (25 °C) throughout. In Experiment 2, adjusting initial moisture to 60 % triggered a 24-h thermophilic surge up to 43 °C, and C/N ratios declined from 21.7 to 26.8 to ∼10 by day 28. Microbial counts increased linearly, indicating activation of indigenous microbiota without inoculum. In Experiment 3, bioaugmentation with <em>Bacillus</em> sp. and <em>Penicillium</em> sp. increased total nitrogen from ∼1.2 % to 1.6 % and accelerated C/N ratio reduction to <17:1, while urea addition suppressed microbial growth and limited humic substance formation to 44.5 %. Across all experiments, ash content increased by 15–25 %, and humification (measured as total humic extract) increased by up to 49.5 % in inoculated treatments. It was concluded that (i) RWS improves moisture balance and aeration, (ii) 60 % moisture and bioaugmentation optimize thermophilic activation and organic matter degradation, and (iii) excessive inorganic nitrogen (urea) can inhibit microbial activity and humification.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103928"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification and characterization of chitinase from Cellulosimicrobium cellulans RS7: Insights into its antimicrobial and anticancer potential 从纤维素微生物RS7中分离纯化几丁质酶及其抑菌和抗癌潜力

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2026-02-01 Epub Date: 2026-01-10 DOI: 10.1016/j.bcab.2026.103925

Gaytri Mahajan, Vivek Chauhan, Rakesh Kumar, Reena Gupta

{"title":"Purification and characterization of chitinase from Cellulosimicrobium cellulans RS7: Insights into its antimicrobial and anticancer potential","authors":"Gaytri Mahajan, Vivek Chauhan, Rakesh Kumar, Reena Gupta","doi":"10.1016/j.bcab.2026.103925","DOIUrl":"10.1016/j.bcab.2026.103925","url":null,"abstract":"<div><div>Chitinase produced by <em>Cellulosimicrobium cellulans</em> RS7 was purified up to 17.28-fold with a yield of 7.02 % and specific activity of 426.9 U/mg. In both Native and SDS-PAGE, the purified chitinase had a single band (47 kDa). It showed maximum activity at 35 °C with sodium phosphate buffer (0.5 M, pH 7.5) in the presence of substrate colloidal chitin (2.0 %) and Mg<sup>2+</sup> ions. The kinetic studies showed that chitinase had <em>K</em>m and <em>V</em>max value of 1.14 mg/mL and 476.19 μmol mg<sup>−1</sup> min<sup>−1</sup>, respectively. Furthermore, 3-D structure of the purified chitinase determined by MALDI- TOF MS technique revealed three active site residues: glutamic acid 292, 296 and aspartic acid 297. The purified chitinase showed cytotoxicity towards RD and L20B cell lines by effectively inhibiting cell growth. Moreover, chitinase produced by <em>C. cellulans</em> RS7 showed low hemolytic activity towards red blood cells. Chitinase also showed antimicrobial activity against <em>S. aureus</em>, <em>S. flexneri</em> and <em>Candida albicans</em> with zone of inhibition 30, 30 and 29 mm respectively.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103925"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Consolidated bio-saccharification (CBS) enhanced hydrogen production via bioaugmentation to eliminate cellobiose inhibition: Investigation on hydrogen production performance and microbial community 强化生物糖化（CBS）通过生物强化来消除纤维二糖抑制来提高制氢：产氢性能和微生物群落的研究

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2026-02-01 Epub Date: 2026-01-13 DOI: 10.1016/j.bcab.2026.103938

Sheng Tao , Song Chengwei , Liu Qianchi , Song Zhiwei , Yang Chunxue , Sun Caiyu , Li Lixin , Huang Linlin , Wang Miao

{"title":"Consolidated bio-saccharification (CBS) enhanced hydrogen production via bioaugmentation to eliminate cellobiose inhibition: Investigation on hydrogen production performance and microbial community","authors":"Sheng Tao , Song Chengwei , Liu Qianchi , Song Zhiwei , Yang Chunxue , Sun Caiyu , Li Lixin , Huang Linlin , Wang Miao","doi":"10.1016/j.bcab.2026.103938","DOIUrl":"10.1016/j.bcab.2026.103938","url":null,"abstract":"<div><div><em>Acetivibrio thermocellus</em> is a key thermophilic, cellulolytic bacterium used in Consolidated bio-saccharification (CBS) for hydrogen production from lignocellulosic biomass. However, the main saccharification product of cellulosic feedstocks, cellobiose, has strong inhibition on <em>A. thermocellus</em> which inhibited cellulase activity and limit overall saccharification and biofuel production efficiency. In this study, a wild-type <em>Acetivibrio thermocellus</em> (strain M3), that could bypass cellobiose inhibition was co-cultured with different hydrogen-producing inocula to test the feasibility of hydrogen production bioaugmentation to eliminate cellobiose inhibition. The results revealed that the addition of <em>A. thermocellus</em> M3 promoted hydrogen production ranging from 42.9 % to 105 %, with the peak hydrogen production obtained from domesticated compost combined with strain M3 (233.02 mL/g). <em>A. thermocellus</em> M3 positively correlated with the hydrogen-producing bacteria. The addition of <em>Acetivibrio thermocellus</em> M3 increased the relative abundance of genes associated with cellulosic feedstocks hydrolysis. Furthermore, the relative abundance of genes associated with stress tolerance and sugar uptake also increased—these genes might help the consolidated bio-saccharification (CBS) hydrogen production system adapt to adverse conditions and thereby alleviate cellobiose inhibition.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"72 ","pages":"Article 103938"},"PeriodicalIF":3.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145974890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unencapsulated and encapsulated Enterococcus faecium E297 added to oat flakes: protective effect against Salmonella Enteritidis in a simulated gastrointestinal system 燕麦片中添加未封装和封装的粪肠球菌E297：在模拟胃肠道系统中对肠炎沙门氏菌的保护作用

IF 3.8

Biocatalysis and agricultural biotechnology Pub Date : 2026-01-01 Epub Date: 2025-12-22 DOI: 10.1016/j.bcab.2025.103896

Rafaela Ansiliero , Taís Nunzio Müller , Stéfani Mallmann , Josiane Marcon , Márcia Bar Schuster , Wladimir Padilha da Silva , Aniela Pinto Kempka , Liziane Schittler Moroni

{"title":"Unencapsulated and encapsulated Enterococcus faecium E297 added to oat flakes: protective effect against Salmonella Enteritidis in a simulated gastrointestinal system","authors":"Rafaela Ansiliero , Taís Nunzio Müller , Stéfani Mallmann , Josiane Marcon , Márcia Bar Schuster , Wladimir Padilha da Silva , Aniela Pinto Kempka , Liziane Schittler Moroni","doi":"10.1016/j.bcab.2025.103896","DOIUrl":"10.1016/j.bcab.2025.103896","url":null,"abstract":"<div><div>This study evaluated the protective effect of <em>Enterococcus faecium</em> E297 against <em>Salmonella</em> Enteritidis in a simulated gastrointestinal system, using both unencapsulated and encapsulated forms via the double emulsion technique (W<sub>1</sub>/O/W<sub>2</sub>) in oat flakes. <em>Enterococcus faecium</em> E297 was encapsulated using extrusion and double emulsion, with encapsulation efficiency being higher for double emulsion (83.99 ± 0.65) compared to extrusion (61.47 ± 4.12 %) after 30 days of storage at 25 °C. Given that oat flakes are stored at room temperature, double emulsion encapsulation was selected for further experiments. <em>Enterococcus faecium</em> E297 encapsulated by double emulsion exhibited greater resistance under simulated gastrointestinal digestion conditions (8.69 ± 0.10 log CFU/mL) compared to the unencapsulated form (5.06 ± 0.06 log CFU/mL). When added to oat flakes and stored at 25 °C for 30 days, both forms of the microorganism showed a reduction in counts, but without significant differences between them, maintaining a cell concentration adequate to be considered probiotic. In oat flakes contaminated with <em>S</em>. Enteritidis and supplemented with <em>E. faecium</em> E297, exposure to simulated gastrointestinal conditions reduced the pathogen concentration by more than 3.5 log CFU/g, regardless of the method used to add the potentially probiotic microorganism. Thus, the food matrix exerted a protective effect on <em>E. faecium</em> E297. It is concluded that the double emulsion technique is effective for encapsulating <em>E. faecium</em> E297 and that its addition to oat flakes provides protection against <em>S</em>. Enteritidis during gastrointestinal digestion.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"71 ","pages":"Article 103896"},"PeriodicalIF":3.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145837940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0