Biocatalysis and agricultural biotechnology最新文献

{"title":"Synthesis of silk sericin/silk fibroin/Moringa oleifera-based hydrogel with antibacterial activity","authors":"Muhammad Iqbal Hidayat ,&nbsp;Andri Hardiansyah ,&nbsp;Riesca Ayu Kusuma Wardhani ,&nbsp;Khoiriah Khoiriah ,&nbsp;Evi Yulianti ,&nbsp;Muhammad Rayhan Izzati Yusuf ,&nbsp;Fahrialdi Fahrialdi","doi":"10.1016/j.bcab.2024.103482","DOIUrl":"10.1016/j.bcab.2024.103482","url":null,"abstract":"<div><div>Hydrogels with excellent antibacterial and mechanical properties represent ideal biomaterials for addressing skin defects, especially in burn wound dressings. In this study, PVA was combined with sericin (PVA/SS), fibroin (PVA/SF), sericin-<em>Moringa oleifera</em> (PVA/SS/MO), and fibroin-<em>Moringa oleifera</em> (PVA/SF/MO) to enhance the stability of the resulting hydrogel and have the antibacterial properties. Hydrogels were synthesized through freezing at −15 °C for 20 h and thawing at 5 °C for 4 h (freeze-thawing method), this process diminished residual waste substances and aligning with green chemistry principles. The FTIR analysis demonstrated that all hydrogel components were successfully blended. The resulting hydrogel had a rough surface with many uniform pores by SEM measurement. Hydrogels showed very hydrophilic properties with lower values of contact angle measurement with a range of 12 and 33°. The swelling properties of the hydrogel showed excellent performance and could absorb water for 5 days. The mechanical properties of the hydrogel were described as tensile strength of 0.29–0.77 MPa, tensile strain of 188–410%, elastic modulus of 0.18–0.72 MPa, and break elongation of 198–413%. Furthermore, PVA/SS/MO and PVA/SF/MO hydrogels display excellent antibacterial properties against Gram-positive (<em>Staphylococcus aureus</em>) and Gram-negative (<em>Escherichia coli</em>) bacteria. Thus, the developing hydrogel is expected to be a future candidate for addressing skin defect problems due to wound infection.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103482"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chamomile waste: A comprehensive insight on phytochemical and safety profile, extraction techniques and potential application","authors":"Nela Drača ,&nbsp;Krunoslav Aladić ,&nbsp;Marija Banožić ,&nbsp;Drago Šubarić ,&nbsp;Stela Jokić ,&nbsp;Ivana Nemet","doi":"10.1016/j.bcab.2024.103468","DOIUrl":"10.1016/j.bcab.2024.103468","url":null,"abstract":"<div><div>Chamomile is widely recognized as both an aromatic and medicinal plant, valued for its exceptional therapeutic properties. Studies has demonstrated that chamomile possesses a range of beneficial effects, including anti-inflammatory, antioxidant, antiseptic, analgesic, antimicrobial, antispasmodic, antiviral, and sedative properties. With the growing global interest in natural ingredients and healthier alternatives to synthetic compounds, there is an increasing demand for accessible and cost-effective bioactive sources. In this context, chamomile waste—such as flower powder or distillation byproducts—emerges as a promising source of bioactive compounds with diverse potential applications. This paper provides a comprehensive overview of the phytochemical composition and safety profile of chamomile waste, highlighting its rich content of polyphenols, polysaccharides, pyrrolizidine alkaloids, and other bioactive compounds. Safety considerations, with particular attention to the presence of pyrrolizidine alkaloids, are also addressed. In addition to discussing its phytochemical composition, the paper reviews various extraction techniques for isolating bioactive compounds from chamomile waste. Traditional solvent extraction methods, including Soxhlet extraction, are compared to green extraction techniques such as supercritical fluid extraction, ultrasound-assisted extraction, and microwave-assisted extraction. Furthermore, the potential applications of chamomile waste in the pharmaceutical, cosmetic, and food industries are explored. Due to its bioactive properties, chamomile waste holds significant promise as an ingredient for skincare products, dietary supplements, and functional foods. The versatility of chamomile waste as a natural source of bioactive compounds presents opportunities for innovative product development and sustainable waste management practices.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103468"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing Lactobacilli strains from Algerian traditional dairy products for probiotic potential benefits","authors":"Malika Bouchibane ,&nbsp;Nabil Touzout ,&nbsp;Djamel Ait Saada ,&nbsp;Abdelkader Elamine Dahou ,&nbsp;Farida Boumediene ,&nbsp;Djahira Hamed ,&nbsp;Selma Toumi ,&nbsp;Abderrahim Cheriguene","doi":"10.1016/j.bcab.2024.103472","DOIUrl":"10.1016/j.bcab.2024.103472","url":null,"abstract":"<div><div>This study explores the probiotic potential of five lactic acid bacteria species (LAB) isolated from traditional Algerian dairy products, with the goal of advancing the development of customized probiotics for innovative applications within the food industry. Tailoring probiotics for specific uses represents a significant advancement in this field. The focus is on five species: <em>Limosilactobacillus fermentum, Lactiplantibacillus pentosus, Lactiplantibacillus plantarum, Lacticaseibacillus paracasei</em> ssp <em>paracasei,</em> and <em>Lactiplantibacillus paraplantarum</em>, all identified through MALDI-TOF mass spectrometry, with confirmation via PCR and 16S rRNA sequencing. The probiotic potential of these strains was evaluated through <em>in vitro</em> experiments assessing tolerance to bile salts and acidity, hydrophobicity, hemolytic activity, sugar fermentation profiles, and resistance to antibiotics and phenols. Results indicated that all the strains displayed significant survival rates at pH 3, ranging from 77.18% ± 1.74–93% ± 1.95. Bile salt tolerance varied among strains, with survival rates between 60.77% ± 2.10 and 91.46% ± 1.68 at a 0.3% concentration, and between 53.91% ± 2.39 and 87.02% ± 2.57 at a 0.5% concentration. Hydrophobicity tests demonstrated moderate adhesion capabilities. Notably, only three strains exhibited resistance to 0.4% phenols, with growth percentages between 29.60% ± 2.03 and 45.97% ± 5.49. Antibiotic susceptibility tests revealed a high sensitivity to various antibiotics, while resistance was noted for cefoxidim, tetracycline, and vancomycin, with the exception of <em>L. fermentum</em>, which remained sensitive to vancomycin. All tested strains were non-hemolytic and capable of fermenting a majority of sugars. Overall, the isolated strains showcase promising probiotic characteristics, providing opportunities for innovation in the food sector by developing new fermented dairy products that meet consumer demands for health and naturalness.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103472"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the therapeutic potential of avocado oil: Insights into obesity metabolism and immune regulation through in vitro models","authors":"Manuela Machado,&nbsp;Eduardo M. Costa,&nbsp;Sara Silva,&nbsp;Ana Maria Gomes,&nbsp;Manuela Pintado","doi":"10.1016/j.bcab.2024.103464","DOIUrl":"10.1016/j.bcab.2024.103464","url":null,"abstract":"<div><div>Avocado oil boasts a compelling nutritional profile characterized by a richness of unsaturated fatty acids and key lipophilic compounds. This study aimed to explore the potential of avocado oil in obesity-related metabolic processes and immune responses modulation in <em>in vitro</em> cellular models. Results obtained unveiled avocado oil capacity to effectively regulate triglyceride accumulation in differentiated 3T3-L1 adipocytes, showcasing a remarkable five-fold increase in adipolysis rate at 15 mg/mL, and an increase in glucose uptake in insulin-stimulated cells. Furthermore, avocado oil exhibited significant immunomodulatory effects in IL-1β-stimulated Caco-2 cells, as it reduced IL-6 (by 11%) and IL-8 (by 12%) production at 10 mg/mL, while only a marginal uptick in TNF-α secretion was observed at the same concentration. These findings underscore the potential of avocado oil to be a beneficial component in functional foods and nutraceuticals targeted at obesity management.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103464"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological evaluation of 2-(3,4-dihydroxyphenyl)-4-((4-methoxyphenyl)imino)-4H-chromene-3,5,7-triol: Design, synthesis, and molecular docking studies","authors":"Udaya Rajesh R,&nbsp;Dhanaraj Sangeetha","doi":"10.1016/j.bcab.2024.103471","DOIUrl":"10.1016/j.bcab.2024.103471","url":null,"abstract":"<div><div>Plant materials are known to contain a category of naturally occurring compounds referred to as flavonoids. Quercetin is classified as a flavonol, which is present in various parts of plants and serves multiple functions within biological systems. This article outlines the synthesis of quercetin Schiff base (Qpa) through a condensation reaction involving quercetin and <em>p</em>-anisidine, followed by characterization using spectroscopic methods such as FTIR, UPLC, ¹H NMR, ¹³C NMR, elemental analysis, and HRMS. The biological applications of Qpa were investigated through antioxidant assays, anti-inflammatory tests, antibacterial evaluations, cytotoxicity assessments, and molecular docking studies. The results indicated that the ¹H NMR and ¹³C NMR spectra exhibited a distinct peak for OCH₃ at 3.66 and 55.73 ppm, respectively. In terms of biological activity, Qpa demonstrated significant antioxidant and anti-inflammatory effects at concentrations of 142.7 μg/mL ± 0.0015 and 247 μg/mL ± 0.0015, respectively. Additionally, Qpa exhibited antibacterial properties comparable to the positive control against both gram-positive and gram-negative bacteria at a concentration of 248 μg. Cytotoxicity studies revealed that Qpa had pronounced effects on MCF-7 and HepG2 cell lines. Furthermore, molecular docking analyses indicated that Qpa displayed high binding energy with AKT, tRNA synthetase, and TNF-α. Future <em>in vivo</em> studies are recommended to elucidate the specific mechanisms of action and to achieve significant results in the pursuit of a novel therapeutic agent.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103471"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UV/EMS mutagenized thermo-alkalophilic polygalacturonase from Glutamicibacter sp.: Physicochemical insights and biotechnological prospects","authors":"Mary Arpana,&nbsp;Aneesa Fasim,&nbsp;Sunil S. More","doi":"10.1016/j.bcab.2024.103465","DOIUrl":"10.1016/j.bcab.2024.103465","url":null,"abstract":"<div><div>The current study discusses the physicochemical properties of an alkalophilic polygalacturonase (PGmut) that was extracted from the UV/EMS mutagenized <em>Glutamicibacter</em> sp. MAIDO R22b-13 strain in comparison to its wildtype (PGwt). Further, its applications in various biotechnology sectors were also assessed. The PGwt and PGmut enzymes were purified by gel permeation chromatography and biochemical analysis determined them to be alkalophilic with pH optima of 10, however mutagenesis enhanced the temperature optima from 37 °C (PGwt) to 50 °C. The molecular weight of both enzymes was confirmed to be 48.67 kDa by SDS-PAGE and LC-MS. PGmut showed higher substrate affinity and velocity, with Km and Vmax of 0.051 mg/ml and 300.12 μmol/min, respectively, compared to 0.072 mg/ml and 205.30 μmol/min for PGwt. Co²⁺ and Mn²⁺ ions enhanced PGmut activity, while Ag²⁺, Cu²⁺, Hg²⁺, and EDTA lowered it. Nevertheless, dialysis-based EDTA removal moderately restored enzyme activity, confirming it to be a metalloenzyme. In addition, PGmut enhanced olive oil production, enabled lycopene extraction from tomato peels, and effectively softened sugarcane bagasse pulp for papermaking. Therefore, this study highlights PGmut's enhanced properties that offers cost-efficient, sustainable, time-saving bioprocess solution, benefiting industry and the environment.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103465"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterologous laccase from the marine environment: Purification, characterization, and degradation of synthetic dyes","authors":"Igor Vinicius Ramos Otero ,&nbsp;Magdalena Haslbeck ,&nbsp;Lara Cavalari Santello ,&nbsp;Henrique Ferreira ,&nbsp;Volker Sieber ,&nbsp;Lara Durães Sette","doi":"10.1016/j.bcab.2024.103485","DOIUrl":"10.1016/j.bcab.2024.103485","url":null,"abstract":"<div><div>The marine-derived fungus <em>Peniophora</em> sp. CBMAI 1063 is a hyper-producer of laccase. Laccases are multicopper oxidases able to oxidize different aromatic compounds while reducing molecular oxygen to water. Several laccases from terrestrial environments have been purified and characterized. However, little is known about marine-derived laccases. In this study, Pnh_Lac1 (Lac1) from the fungus <em>Peniophora</em> sp. CBMAI 1063 was heterologously expressed in <em>Pichia pastoris</em>, purified, characterized, and used for the degradation/detoxification of synthetic dyes. Lac1 (∼72 kDa) exhibited optimal activity at 60 °C and pH 3, with good thermostability (T₅₀^1h = 56 °C) and high tolerance to metal ions and organic solvents. Lac 1 decolorized/degraded different classes of dyes, under low enzyme concentrations (0.2–0.02 U mL^-1), with an excellent performance regarding the decolorization of Indigo Carmine (93% after 2 h) in the presence of syringaldehyde. Additionally, 65% of the azo dye Reactive Black 5 was degraded by the Lac1-mediator system into lower molecular weight metabolites, with a significant reduction in phytotoxicity. These results demonstrate that the marine-derived Lac1 is a fungal laccase highly active under low concentration, with the potential to mitigate environmental pollutants on biodegradation strategies based on biocatalysis.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103485"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in conversion technologies for biofuels from wheat and corn straws","authors":"Elisiane Rocha Lufan Prado,&nbsp;Rafael Cardoso Rial","doi":"10.1016/j.bcab.2024.103481","DOIUrl":"10.1016/j.bcab.2024.103481","url":null,"abstract":"<div><div>This work reviews recent technologies and processes for the production of biofuels from wheat and corn straw, highlighting their potential as renewable energy sources. These often underutilized agricultural residues offer a significant opportunity for the production of bioethanol, biogas, and bio-oil, contributing to the diversification of the energy matrix and the mitigation of greenhouse gas emissions. The analysis details the impact of the chemical constituents of these straws on the efficiency of conversion processes, such as lignin and phenolic acids, which can inhibit the production of bioethanol and biogas. Among the technological advances, methods such as microwave-assisted pyrolysis and the use of ionic liquids have shown significant improvements in conversion efficiency, removing inhibitors and increasing biofuel productivity. However, these processes still face challenges related to scalability and costs. Additionally, the use of wheat and corn straw can add economic value for farmers and promote rural development, creating new sources of income and encouraging a circular economy. Overall, this study concludes that second-generation biofuels derived from wheat and corn straw hold immense potential to address environmental and economic challenges, but their large-scale adoption requires continued technological innovation and supportive public policies.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103481"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential use of Ecdysone in protecting wheat (Triticum aestivum) germination under cadmium stress","authors":"Mostafa Lamhamdi ,&nbsp;Saliha Gharbi ,&nbsp;Mohamed El Arras ,&nbsp;Ouiam El Galiou ,&nbsp;Hamza El Moudden ,&nbsp;Learn-Han Lee ,&nbsp;Abdelhakim Bouyahya ,&nbsp;Ahmed Bakrim","doi":"10.1016/j.bcab.2024.103479","DOIUrl":"10.1016/j.bcab.2024.103479","url":null,"abstract":"<div><div>The purpose of this work is to explore the potential of Ecdysone (Ecd) in protecting wheat seedlings from the toxic effects of cadmium (Cd). Various growth parameters such as germination, tolerance index, plant biomass, and vigor index were assessed. Additionally, germination metabolic parameters including soluble sugar content, soluble proteins, free amino acids, amylase, and protease activities were measured. During this study, we investigated the stress markers content, proline, chlorophyll, and antioxidant enzymes. The amount of Cd uptake and its distribution at the cellular level were evaluated. In the absence of pretreatment. The results showed that high concentrations of Cd (0.1 and 0.5 mM) caused a decrease in germination (13%–35%) and growth (24%–53%). In addition, indicators of oxidative stress were observed, suggesting an alteration of physiological processes. Key germination enzymes α-amylase and proteases activities decreased with increasing Cd concentration, leading to a reduction in solubles sugar (48%–64%) and amino acid (55%–64%) levels. Ecdysone pretreatment (10 μM) significantly mitigated Cd toxicity in young plants by decreasing its absorption (71%–40% in leaf and root respectively at 0.5 mM Cd), alleviating oxidative stress (36%–43% reducing MDA), and enhancing detoxification processes. In addition, Ecd pretreatment maintained normal germination, tolerance, biomass, and metabolism in the presence of Cd. According to our results, Ecdysone ensures the preservation of oxidative balance and promotes wheat germination metabolism.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103479"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigation of H2O2 induced Reactive Oxygen species and Proinflammatory cytokines by Buckwheat (Fagopyrum tataricum) rutin in cultured RAW264.7 cells","authors":"Dennis Soosai ,&nbsp;R. Ravindhran ,&nbsp;Elumalai Perumal ,&nbsp;C. Sheela Sasikumar ,&nbsp;Charles P ,&nbsp;Leo Arockia Raj S","doi":"10.1016/j.bcab.2024.103462","DOIUrl":"10.1016/j.bcab.2024.103462","url":null,"abstract":"<div><h3>Background</h3><div>Buckwheat rutin, sourced from the Himalayan region of Nepal, exhibits significant free radical-scavenging potential. The rutin fraction derived from a tartary buckwheat seeds cultivar NGRC03731 exerts protective effects by preventing cellular damage and regulating proinflammatory cytokines in the mouse macrophage cell line RAW264.7.</div></div><div><h3>Methods</h3><div>The rutin fraction antioxidant properties were investigated using DPPH and ABTS methods in a cell-free system. RAW 264.7 cell viability following exposure to rutin fraction and standard rutin in the presence or absence of H₂O₂ was determined using the MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay. Free radical generation was evaluated through DCFDA staining. Pro-inflammatory cytokine gene expression levels were assessed using real-time PCR, while the corresponding protein levels were measured using Enzyme linked immunosorbent assay (ELISA).</div></div><div><h3>Results</h3><div>The rutin fraction markedly reduced NO and PGE2 levels. The viability of RAW 264.7 cells exposed to H₂O₂ and co-treated with the rutin fraction retained cell viability. Moreover, the rutin fraction alone (200 μg/mL) did not alter the morphology of RAW 264.7 cells, and reduced cellular damage in the presence of H₂O₂. TNF-α, COX-2a, IL-1, IL-6, IL-8, and IL-10 gene expression was significantly downregulated as well the cytokine protein levels of TNF-α, IL-1β, IL-8, IL-6, and IL-10 were markedly down regulated in RAW 264.7 cells treated with the rutin fraction.</div></div><div><h3>Conclusion</h3><div>The results of our study demonstrate the remarkable anti-inflammatory properties of the buckwheat rutin fraction in suppressing inflammatory responses.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"63 ","pages":"Article 103462"},"PeriodicalIF":3.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}