The assessment of antimicrobial and antibiofilm activities, molecular docking studies, and the photodegradation of methylene blue by green-fabricated ZnO nanoparticles using the plant extract of Helichrysum noeanum boiss

IF 3.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology Pub Date : 2025-08-30 DOI:10.1016/j.bcab.2025.103755

Suzan Sahin Dogan , Duygu Aydin , Bugrahan Emsen , Burak Surmen

{"title":"The assessment of antimicrobial and antibiofilm activities, molecular docking studies, and the photodegradation of methylene blue by green-fabricated ZnO nanoparticles using the plant extract of Helichrysum noeanum boiss","authors":"Suzan Sahin Dogan , Duygu Aydin , Bugrahan Emsen , Burak Surmen","doi":"10.1016/j.bcab.2025.103755","DOIUrl":null,"url":null,"abstract":"<div><div>In recent years, natural reducing agents have been studied worldwide to reduce harmful effects of toxic chemicals utilized in nanoparticle production. Zinc oxide nanoparticles (ZnO NPs) have been prepared for their photocatalytic potential in treating industrial dyes, for example methylene blue (MB). Therefore, the present study reports synthesizing and assessing antimicrobial effectiveness of ZnO NPs biosynthesized with an endemic plant <em>Helichrysum noeanum</em> Boiss (<em>H. noeanum</em>). Optical, morphological, and surface area features of synthesized ZnO NPs were evaluated utilizing UV–visible spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and Energy dispersive X-ray analysis (EDX) and X-ray diffraction (XRD), and determined the chemical composition and purity ZnO NPs as well as DLS and zeta potential analysis. The interactions between phytoconstituents in aqueous extract of <em>H. noeanum</em> and ZnO NPs were identified from FT-IR spectra. Dye degradation efficiency of ZnO NPs (5 mg/mL) was found as 81 % against MB under sunlight. Additionally, minimum inhibitory concentration (MIC) values for, <em>S. aureus</em> ATCC 29213, <em>E. coli</em> ATCC 43895, <em>B. cereus</em> NRRL B-3711<em>, P. aeruginosa</em> ATCC 27853, and <em>S. typhi</em> were 75, 50, 50, 100, 50 μg/mL, respectively. Furthermore, ZnO NPs at a concentration of 100 μg/mL reduced 94 % of the biofilm formed by P. aeruginosa. Also, molecular docking was performed to evaluate the binding interactions between ZnO NPs and protein targets involved in microbial pathogenesis and biofilm activity, including PaAP, elastase, and pqsA. Therefore, this study offers a straightforward and effective method for synthesizing ZnO NPs mediated with <em>H. noeanum</em> with potent antimicrobial activity and for wastewater treatment by degrading various toxic dyes.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"69 ","pages":"Article 103755"},"PeriodicalIF":3.8000,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biocatalysis and agricultural biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1878818125002683","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

In recent years, natural reducing agents have been studied worldwide to reduce harmful effects of toxic chemicals utilized in nanoparticle production. Zinc oxide nanoparticles (ZnO NPs) have been prepared for their photocatalytic potential in treating industrial dyes, for example methylene blue (MB). Therefore, the present study reports synthesizing and assessing antimicrobial effectiveness of ZnO NPs biosynthesized with an endemic plant Helichrysum noeanum Boiss (H. noeanum). Optical, morphological, and surface area features of synthesized ZnO NPs were evaluated utilizing UV–visible spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and Energy dispersive X-ray analysis (EDX) and X-ray diffraction (XRD), and determined the chemical composition and purity ZnO NPs as well as DLS and zeta potential analysis. The interactions between phytoconstituents in aqueous extract of H. noeanum and ZnO NPs were identified from FT-IR spectra. Dye degradation efficiency of ZnO NPs (5 mg/mL) was found as 81 % against MB under sunlight. Additionally, minimum inhibitory concentration (MIC) values for, S. aureus ATCC 29213, E. coli ATCC 43895, B. cereus NRRL B-3711, P. aeruginosa ATCC 27853, and S. typhi were 75, 50, 50, 100, 50 μg/mL, respectively. Furthermore, ZnO NPs at a concentration of 100 μg/mL reduced 94 % of the biofilm formed by P. aeruginosa. Also, molecular docking was performed to evaluate the binding interactions between ZnO NPs and protein targets involved in microbial pathogenesis and biofilm activity, including PaAP, elastase, and pqsA. Therefore, this study offers a straightforward and effective method for synthesizing ZnO NPs mediated with H. noeanum with potent antimicrobial activity and for wastewater treatment by degrading various toxic dyes.

查看原文本刊更多论文

以蜡菊植物提取物为原料制备的氧化锌纳米颗粒对亚甲基蓝的光降解作用、抗菌活性和抗生物膜活性的评价、分子对接研究

近年来，世界各国对天然还原剂进行了研究，以减少纳米颗粒生产中使用的有毒化学品的有害影响。氧化锌纳米颗粒（ZnO NPs）在处理工业染料（如亚甲基蓝（MB））方面具有光催化潜力。因此，本研究报道了用特有植物蜡菊（Helichrysum noeanum Boiss）生物合成的氧化锌NPs的合成和抗菌效果评估。利用紫外-可见光谱、透射电子显微镜（TEM）、扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、能量色散x射线分析（EDX）和x射线衍射（XRD）对合成ZnO NPs的光学、形貌和表面积特征进行了评价，确定了ZnO NPs的化学成分和纯度，并进行了DLS和zeta电位分析。利用傅里叶红外光谱（FT-IR）鉴定了紫荆水提液中植物成分与氧化锌NPs之间的相互作用。氧化锌纳米粒子（5 mg/mL）在日光下对MB的降解率为81%。对金黄色葡萄球菌ATCC 29213、大肠杆菌ATCC 43895、蜡样芽孢杆菌NRRL B-3711、铜绿假单胞菌ATCC 27853、伤寒沙门氏菌的最小抑制浓度（MIC）分别为75、50、50、100、50 μg/mL。此外，100 μg/mL浓度的ZnO NPs使铜绿假单胞菌形成的生物膜减少了94%。此外，还进行了分子对接，以评估ZnO NPs与参与微生物发病和生物膜活性的蛋白靶点（包括PaAP、elastase和pqsA）之间的结合相互作用。因此，本研究提供了一种简单有效的方法来合成具有强抑菌活性的紫花草介导的ZnO NPs，并通过降解各种有毒染料来处理废水。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

7.70

自引率

2.50%

发文量

308

审稿时长

48 days

期刊介绍： Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.